CN102274386A - Method for extracting total isoflavones from Allium mongolicum Regel - Google Patents
Method for extracting total isoflavones from Allium mongolicum Regel Download PDFInfo
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- CN102274386A CN102274386A CN2011102263879A CN201110226387A CN102274386A CN 102274386 A CN102274386 A CN 102274386A CN 2011102263879 A CN2011102263879 A CN 2011102263879A CN 201110226387 A CN201110226387 A CN 201110226387A CN 102274386 A CN102274386 A CN 102274386A
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- goldball onion
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- total isoflavone
- extracting total
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Abstract
The invention discloses a method for extracting total isoflavones from Allium mongolicum Regel, which comprises the following steps: pulverizing Allium mongolicum Regel which serves as the raw material, putting the pulverized Allium mongolicum Regel into a liquid dynamic continuous reflux extraction device to carry out alcohol extraction, merging the extracting solutions, recycling the alcohol, purifying by membrane separation, and passing the filtrate through a macroporous resin; and after the adsorption finishes, eluting with water and alcoholic solution, collecting the target fraction, concentrating, and drying to obtain the Allium mongolicum Regel total isoflavones. The invention has the advantages of simple technological process, high extraction efficiency and high product purity, and is very convenient for industrial production; and since edible alcohol is used as the solvent in the preparation process, the invention also has the advantages of less pollution and low cost.
Description
Technical field
The invention belongs to the Chinese medicine extraction technical field, relate to a kind of method of from goldball onion, extracting total isoflavone.
Background technology
Goldball onion belongs to Angiospermae Liliaceae allium, and formal name used at school Mongolia fragrant-flowered garlic is called Mongolian Herba Alii fistulosi, covered Herba Alii fistulosi, Herba Allii prattii, Herba Alii fistulosi, is a kind of plant of vitality tanacity.Goldball onion mainly is distributed in the desert steppe and the sand ground on ground such as Xinjiang, Qinghai, Gansu, Ningxia, the Inner Mongol, is characterized in that cold-resistant, drought resisting, adaptive capacity are strong.Goldball onion has high nutritive value and medical value, and its aerial parts can be used as medicine, and special efficacy such as have blood pressure lowering, blood fat reducing, appetite-stimulating indigestion-relieving, body-building tonifying YANG, treat constipation is called as " Ganoderma in the dish ".In addition, goldball onion has high feeding value, and document announcement is arranged, and goldball onion will be higher than international one-level Herba Medicaginis as its nutritive value of herbage.
The goldball onion main active is flavone compound, polysaccharide etc.At present, do not see the relevant patent report of goldball onion total isoflavone extraction separation method as yet.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of method of extracting total isoflavone from goldball onion is provided, this method is simple to operate, extraction ratio is high.
To achieve these goals, technical scheme of the present invention is as follows:
A kind of method of extracting total isoflavone from goldball onion is characterized in that concrete steps are as follows:
(1) extract: with the goldball onion is raw material, pulverizes, and places the dynamic continuous counter-flow extraction equipment of liquid, and the edible ethanol solution with 60-80% under 35-70 ℃ extraction temperature, extracts merge extractive liquid, 2-3 time;
(2) membrance separation: said extracted liquid reclaims ethanol, and the centrifuging and taking supernatant carries out microfiltration, ultrafiltration successively in the rolled film separation equipment, obtain permeate;
(3) column chromatography: macroporous resin on the permeate, after absorption is finished, wash with water earlier to fluid injection is approaching colourless down, discard water lotion, reuse 60-70% edible ethanol eluting is collected ethanol elution, and concentrate drying obtains the goldball onion total isoflavone.
The dynamic continuous flow upstream device of liquid is that a plurality of extraction element serial or parallel connections or connection in series-parallel are share in the described step (1), and extract the alcoholic solution consumption is 3-5 times (v/w) of goldball onion quality at every turn.
Membrane material is poly (ether sulfone) film or polysulfone membrane in the described step (2), and the micro-filtration membrane aperture is 0.2-0.45 μ m, and the ultrafilter membrane molecular cut off is 30000-1000, and operating pressure is 0.1-0.15Mpa.
Macroporous adsorbent resin in the described step (3) is AB-8, HPD100 or ADS-7 type.
The present invention has the following advantages:
(1) utilizes the dynamic Continuous Countercurrent Extraction technology of liquid, constantly extracts active ingredients is come out, shortened the consumption of extracting cycle and solvent greatly by the circulation extracting solution.
(2) adopt technology such as microfiltration, ultrafiltration, extracting solution is carried out membrance separation, improve its purity, and be that the preceding sample solution of macroporous resin column chromatography is purified, alleviate infringement, prolong the life cycle of resin resin.
(3) technology of the present invention is simple, can be fit to industrialized great production, and in the preparation process, only adopting water and food grade ethanol is solvent, pollutes and lacks, and cost is low.
Further specify the present invention below in conjunction with the specific embodiment, but the scope of protection of present invention is not limited to the following example.
The specific embodiment
Embodiment 1:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 25L80%, under 35 ℃ extraction temperature, extract 2 times, each 4h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.15Mpa, obtains permeate, last AB-8 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 60% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 90.1%.
Embodiment 2:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 25L60%, under 40 ℃ extraction temperature, extract 3 times, each 3h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.1Mpa, obtains permeate, last ADS-7 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 60% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 92.1%.
Embodiment 3:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 15L80%, under 70 ℃ extraction temperature, extract 2 times, each 3h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.15Mpa, obtains permeate, last AB-8 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 70% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 93.0%.
Embodiment 4:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 20L60%, under 65 ℃ extraction temperature, extract 2 times, each 4h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.15Mpa, obtains permeate, last HPD100 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 60% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 90.2%.
Embodiment 5:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 25L650%, under 50 ℃ extraction temperature, extract 2 times, each 3h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.15Mpa, obtains permeate, last AB-8 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 70% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 90.0%.
Embodiment 6:
With the goldball onion is raw material, pulverizes, and gets 5kg, on average place 5 placed in-line extraction elements, add the edible ethanol solution of 15L80%, under 35 ℃ extraction temperature, extract 3 times, each 2h, merge extractive liquid, reclaims ethanol, the centrifuging and taking supernatant, in the rolled film separation equipment, carry out microfiltration successively, ultrafiltration, operating pressure is 0.1Mpa, obtains permeate, last ADS-7 type macroporous resin, after absorption is finished, wash with water earlier, discard water lotion to fluid injection is approaching colourless down, reuse 70% edible ethanol eluting, collect ethanol elution, concentrate drying obtains the goldball onion total isoflavone, and extraction ratio is 90.5%.
Claims (4)
1. method of extracting total isoflavone from goldball onion is characterized in that concrete steps are as follows:
(1) extract: with the goldball onion is raw material, pulverizes, and places the dynamic continuous counter-flow extraction equipment of liquid, and the edible ethanol solution with 60-80% under 35-70 ℃ extraction temperature, extracts merge extractive liquid, 2-3 time;
(2) membrance separation: said extracted liquid reclaims ethanol, and the centrifuging and taking supernatant carries out microfiltration, ultrafiltration successively in the rolled film separation equipment, obtain permeate;
(3) column chromatography: macroporous resin on the permeate, after absorption is finished, wash with water earlier to fluid injection is approaching colourless down, discard water lotion, reuse 60-70% edible ethanol eluting is collected ethanol elution, and concentrate drying obtains the goldball onion total isoflavone.
2. a kind of method of from goldball onion, extracting total isoflavone according to claim 1, it is characterized in that: the dynamic continuous flow upstream device of liquid is that a plurality of extraction element serial or parallel connections or connection in series-parallel are share in the described step (1), and extract the alcoholic solution consumption is 3-5 times (v/w) of goldball onion quality at every turn.
3. a kind of method of from goldball onion, extracting total isoflavone according to claim 1, it is characterized in that: membrane material is poly (ether sulfone) film or polysulfone membrane in the described step (2), the micro-filtration membrane aperture is 0.2-0.45 μ m, the ultrafilter membrane molecular cut off is 30000-1000, and operating pressure is 0.1-0.15Mpa.
4. a kind of method of extracting total isoflavone from goldball onion according to claim 1, it is characterized in that: the macroporous adsorbent resin in the described step (3) is AB-8, HPD100 or ADS-7 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011102263879A CN102274386A (en) | 2011-08-09 | 2011-08-09 | Method for extracting total isoflavones from Allium mongolicum Regel |
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| CN2011102263879A CN102274386A (en) | 2011-08-09 | 2011-08-09 | Method for extracting total isoflavones from Allium mongolicum Regel |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520449A (en) * | 2013-10-01 | 2014-01-22 | 李官浩 | Preparation method and application of onionskin flavones |
| CN106866599A (en) * | 2017-01-05 | 2017-06-20 | 李官浩 | A kind of ferment onion skin functional product and preparation method thereof |
| CN110507749A (en) * | 2019-08-21 | 2019-11-29 | 南京晓庄学院 | A kind of Allium mongolicum Regel antineoplastic extract and its preparation method and application |
| CN114209727A (en) * | 2021-11-05 | 2022-03-22 | 浙江工业大学 | Method for extracting total flavonoids of chrysanthemum morifolium ramat |
-
2011
- 2011-08-09 CN CN2011102263879A patent/CN102274386A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520449A (en) * | 2013-10-01 | 2014-01-22 | 李官浩 | Preparation method and application of onionskin flavones |
| CN106866599A (en) * | 2017-01-05 | 2017-06-20 | 李官浩 | A kind of ferment onion skin functional product and preparation method thereof |
| CN106866599B (en) * | 2017-01-05 | 2017-09-29 | 李官浩 | A kind of ferment onion skin functional product and preparation method thereof |
| CN110507749A (en) * | 2019-08-21 | 2019-11-29 | 南京晓庄学院 | A kind of Allium mongolicum Regel antineoplastic extract and its preparation method and application |
| CN110507749B (en) * | 2019-08-21 | 2021-10-01 | 南京晓庄学院 | Mongolian leek anti-tumor extract and preparation method and application thereof |
| CN114209727A (en) * | 2021-11-05 | 2022-03-22 | 浙江工业大学 | Method for extracting total flavonoids of chrysanthemum morifolium ramat |
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Application publication date: 20111214 |