CN220246113U - Unidirectional bioreactor sampling device - Google Patents
Unidirectional bioreactor sampling device Download PDFInfo
- Publication number
- CN220246113U CN220246113U CN202321736215.0U CN202321736215U CN220246113U CN 220246113 U CN220246113 U CN 220246113U CN 202321736215 U CN202321736215 U CN 202321736215U CN 220246113 U CN220246113 U CN 220246113U
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- bioreactor
- check valve
- sampling
- sampler
- needleless
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- 238000005070 sampling Methods 0.000 title claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 46
- 238000003756 stirring Methods 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a one-way bioreactor sampling device, which comprises a bioreactor, a sampling pipeline, a check valve, a needleless sampler and a sample collection container; the sampling port of the bioreactor is connected with the liquid inlet end of the check valve through a sampling pipeline, so that biological liquid in the bioreactor can only flow out through the check valve and cannot flow back into the bioreactor through the check valve; the liquid outlet end of the check valve is connected with the liquid inlet end of the needleless sampler; the liquid outlet end of the needleless sampler is connected with a sample collection container; according to the utility model, the check valve is arranged at the sampling port of the bioreactor, so that a sample can only flow into the sample collecting container along a single direction through the needleless sampler, liquid backflow is avoided, bacterial pollution caused by sampling is avoided by 100%, sampling operation is simple, convenient and quick, no special requirement is imposed on the technology of operators, and sampling can be efficiently completed only by 30-60 seconds.
Description
Technical Field
The utility model relates to the technical field of biological and medical related experimental equipment, in particular to a sampling device of a unidirectional bioreactor.
Background
Bioreactor culture is a mainstream mode of biological drug research and development and production, and frequent sampling and detection of parameters such as cells, metabolism, biochemistry, expression and the like are required in the culture process.
The existing sampling has modes of a disposable multi-connection sampling bag, a sampling bottle, a needleless sampler and the like, but all have respective problems; the multi-connected sampling bag has high cost and complex operation, has high requirement on the sample amount in sampling, and is only suitable for large-scale bioreactors; the sampling bottle has complicated operation, and repeatedly sampling negative pressure and pressurizing the sample during sampling to make the biochemical characteristics (such as pH and pCO) 2 、PO 2 ) The influence is larger; the needleless sampler operation has high requirements on personnel operation, the sample is easy to reflux due to careless operation, the risk is extremely high, bacterial pollution is easily caused in sampling, and great time and money cost are wasted.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provide a unidirectional bioreactor sampling device which can effectively prevent a needleless sampler from generating a reflux phenomenon, 100% avoids bacterial pollution caused by sampling and has the advantages of simple structure, convenient and quick operation and the like.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a unidirectional bioreactor sampling device, which comprises a bioreactor, a sampling pipeline, a check valve, a needleless sampler and a sample collection container;
the sampling port of the bioreactor is connected with the liquid inlet end of the check valve through a sampling pipeline, so that biological liquid in the bioreactor can only flow out through the check valve and cannot flow back into the bioreactor through the check valve;
the liquid outlet end of the check valve is connected with the liquid inlet end of the needleless sampler;
the liquid outlet end of the needleless sampler is connected with a sample collection container.
Preferably, the liquid outlet end of the check valve is fixedly connected with the liquid inlet end of the needleless sampler to form a part.
Preferably, the liquid outlet end of the check valve is detachably connected with the liquid inlet end of the needleless sampler by a reverse hook connector or a luer connector.
Preferably, the liquid outlet end of the check valve is connected with the liquid inlet end of the needleless sampler by adopting a connecting pipeline.
Preferably, two ends of the connecting pipeline are detachably connected with the liquid outlet end of the check valve and the liquid inlet end of the needleless sampler by a reverse hook connector or a luer connector respectively.
Preferably, the connecting pipeline is a silica gel pipe or a connecting joint.
Preferably, the bioreactor is one of a stirring type, a torrent type or a swing type.
Due to the application of the technical scheme, compared with the prior art, the utility model has the following advantages:
1. according to the utility model, the check valve is arranged at the sampling port of the bioreactor, so that a sample can only flow into the sample collecting container along a single direction through the needleless sampler, liquid backflow is avoided, and bacterial pollution caused by sampling is avoided by 100%;
2. the utility model has simple, convenient and quick sampling operation, has no special requirement on the technology of operators, and can efficiently finish the sampling in 30-60 seconds.
Drawings
The technical scheme of the utility model is further described below with reference to the accompanying drawings:
FIG. 1 is a schematic diagram of the overall structure of a sampling device for a unidirectional bioreactor according to the present utility model;
FIG. 2 is a schematic diagram of the overall structure of the unidirectional bioreactor sampling apparatus according to the present utility model.
Wherein: 1. a bioreactor; 2. a sampling pipeline; 3. a check valve; 4. a connecting pipeline; 5. a needleless sampler; 6. a sample collection container; 7. the direction of fluid flow.
Detailed Description
The utility model will be described in further detail with reference to the accompanying drawings and specific examples.
Embodiment one:
FIG. 1 is a schematic illustration of a one-way bioreactor sampling device according to the present utility model, comprising a bioreactor 1, a sampling line 2, a check valve 3, a needle-free sampler 5 and a sample collection vessel 6; the sampling port of the bioreactor 1 is connected with the liquid inlet end of the check valve 3 through a sampling pipeline 2, so that biological liquid in the bioreactor 1 can only flow out through the check valve 3 and cannot flow back into the bioreactor 1 through the check valve 3; the liquid outlet end of the check valve 3 is connected with the liquid inlet end of the needleless sampler 5; the liquid outlet end of the needleless sampler 5 is connected with a sample collection container 6; the bioreactor 1 comprises a plurality of types of bioreactors 1 such as stirring type bioreactors 1, torrent type bioreactors 1 and swing type bioreactors; the sample collection container 6 may be a syringe or other withdrawal device; the liquid outlet end of the check valve 3 is fixedly connected with the liquid inlet end of the needleless sampler 5 to form a part, so that the structure is more compact, and the assembly is convenient.
When in use, the utility model is characterized in that: the sample collection container 6 or other extraction device is inserted into the needleless sampler 5 to extract outwards, and the fluid flows in the direction 7 from the end of the bioreactor 1 to the sampling end, so that the fluid is non-return and cannot flow back to the bioreactor 1 from the sampling end, and therefore, the sample in the bioreactor 1 enters the check valve 3 from the sampling pipeline 2, enters the needleless sampler 5 through the pipeline 4 and then enters the sample collection container 6, and the sampling is completed.
Embodiment two:
unlike the first embodiment, the following is: the liquid outlet end of the check valve 3 is detachably connected with the liquid inlet end of the needleless sampler 5 by a reverse hook connector or a luer connector, so that the check valve is convenient to detach according to the use situation.
Embodiment III:
as shown in fig. 2, a first difference from the embodiment is that: the liquid outlet end of the check valve 3 is connected with the liquid inlet end of the needleless sampler 5 through a connecting pipeline 4, the length between the check valve 3 and the needleless sampler 5 can be increased through the connecting pipeline 4, and the device is suitable for long-distance sampling.
Embodiment four:
on the basis of the third embodiment, further, two ends of the connecting pipeline 4 are detachably connected with the liquid outlet end of the check valve 3 and the liquid inlet end of the needleless sampler 5 respectively by adopting a reverse hook connector or a luer connector, so that the disassembly is convenient according to the use occasion.
Fifth embodiment:
on the basis of the third embodiment, further, the connecting pipeline 4 is a silicone tube or a connecting joint, and can be selected according to the use situation.
The foregoing is merely a specific application example of the present utility model, and the protection scope of the present utility model is not limited in any way. All technical schemes formed by equivalent transformation or equivalent substitution fall within the protection scope of the utility model.
Claims (7)
1. A unidirectional bioreactor sampling device, characterized in that: comprises a bioreactor, a sampling pipeline, a check valve, a needleless sampler and a sample collection container;
the sampling port of the bioreactor is connected with the liquid inlet end of the check valve through a sampling pipeline, so that biological liquid in the bioreactor can only flow out through the check valve and cannot flow back into the bioreactor through the check valve;
the liquid outlet end of the check valve is connected with the liquid inlet end of the needleless sampler;
the liquid outlet end of the needleless sampler is connected with a sample collection container.
2. The unidirectional bioreactor sampling apparatus of claim 1, wherein: the liquid outlet end of the check valve is fixedly connected with the liquid inlet end of the needleless sampler to form a component.
3. The unidirectional bioreactor sampling apparatus of claim 1, wherein: the liquid outlet end of the check valve is detachably connected with the liquid inlet end of the needleless sampler by a reverse hook connector or a luer connector.
4. The unidirectional bioreactor sampling apparatus of claim 1, wherein: the liquid outlet end of the check valve is connected with the liquid inlet end of the needleless sampler by adopting a connecting pipeline.
5. The unidirectional bioreactor sampling apparatus of claim 4 wherein: and two ends of the connecting pipeline are detachably connected with a liquid outlet end of the check valve and a liquid inlet end of the needleless sampler by adopting a reverse hook connector or a luer connector respectively.
6. The unidirectional bioreactor sampling apparatus of claim 4 wherein: the connecting pipeline is a silica gel pipe or a connecting joint.
7. The unidirectional bioreactor sampling apparatus of any one of claims 1 to 6, wherein: the bioreactor is one of a stirring type, a torrent type or a swing type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321736215.0U CN220246113U (en) | 2023-07-04 | 2023-07-04 | Unidirectional bioreactor sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321736215.0U CN220246113U (en) | 2023-07-04 | 2023-07-04 | Unidirectional bioreactor sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220246113U true CN220246113U (en) | 2023-12-26 |
Family
ID=89266050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321736215.0U Active CN220246113U (en) | 2023-07-04 | 2023-07-04 | Unidirectional bioreactor sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220246113U (en) |
-
2023
- 2023-07-04 CN CN202321736215.0U patent/CN220246113U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240703 Address after: Room 601, No. 1, Lane 66, Huayuan Road, Hongkou District, Shanghai, 200000 Patentee after: Yishike (Shanghai) Enterprise Development Co.,Ltd. Country or region after: China Address before: Room 101, Building 1, No. 399 Suhong East Road, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, Suzhou City, Jiangsu Province, 215000 Patentee before: Yishike (Jiangsu) Life Science and Technology Co.,Ltd. Country or region before: China |