CN217155954U - Sampling device of bioreactor - Google Patents
Sampling device of bioreactor Download PDFInfo
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- CN217155954U CN217155954U CN202122437087.7U CN202122437087U CN217155954U CN 217155954 U CN217155954 U CN 217155954U CN 202122437087 U CN202122437087 U CN 202122437087U CN 217155954 U CN217155954 U CN 217155954U
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- branch pipe
- bioreactor
- responsible
- sampling device
- silicone tube
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Abstract
The utility model discloses a sampling device of bioreactor, this sampling device of bioreactor includes sample pipeline, air cleaner, the thumb clamp, luer connects and disposable syringe, sample pipeline is including being responsible for and the branch pipe, be responsible for the below and connect first silicone tube, be responsible for the top and connect the second silicone tube, be responsible for the second silicone tube connecting branch pipe that the top is connected, air cleaner is connected to branch pipe one end, branch pipe end-to-end connection luer connects, the thumb clamp presss from both sides on the branch pipe, disposable syringe and luer connect are the separation, disposable syringe is used for extracting the cell culture liquid. This sampling device has avoided culture solution and external environment direct contact, has reduced by external environment pollution's risk, has realized aseptic sample, has made things convenient for personnel to bioreactor's sample operation.
Description
Technical Field
The utility model relates to a cell culture process field of a bioreactor, in particular to a sampling device of a bioreactor.
Background
When a bioreactor is used in a laboratory for cell batch culture, the cell growth density, the survival rate and the metabolic condition must be detected every day. This just needs to carry out the sampling operation to the reactor every day, and sampling device and process are crucial, in case operate improperly, for example liquid leaks, liquid refluence etc. makes the pipeline suffer pollution to whole reactor culture solution will scrap, causes great loss.
Therefore, a need exists for a sampling device for a bioreactor.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a sampling device of a bioreactor.
The utility model provides a sampling device of bioreactor includes sample pipeline, air cleaner, thumb clamp, luer joint and disposable syringe, sample pipeline is including being responsible for and the branch pipe, be responsible for the below and connect first silicone tube, be responsible for the top and connect the second silicone tube, be responsible for the second silicone tube connecting branch pipe that the top is connected, air cleaner is connected to branch pipe one end, branch pipe end-to-end connection luer joint, the thumb clamp presss from both sides on the branch pipe, disposable syringe and luer joint are the separation.
In the above scheme, the silica gel tube outside the reaction tank is tightened by the ribbon, so that the air tightness of the pipeline is further ensured.
In the above scheme, the branch pipe is a silica gel branch pipe, and the silica gel branch pipe is a hose made of silica gel materials.
In the above scheme, the main pipe is a stainless steel pipe.
The utility model has the advantages and the beneficial effects that: the utility model provides a sampling device of a bioreactor. This sampling device passes through the syringe extraction sample, in time closes the interface, has avoided culture solution and external environment direct contact, has reduced the risk of being polluted by external environment, has realized aseptic sample, has made things convenient for personnel to bioreactor's sample operation.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
Fig. 1 is a schematic structural diagram of the present invention.
In the figure: 1. reaction pot 2, main pipe 3, first silicone tube 4, second silicone tube 5, third silicone tube 6, fourth silicone tube 7, fifth silicone tube 8, air filter 9, luer 10, disposable syringe 11, thumb clamp 12, thumb clamp 13, thumb clamp 14, thumb clamp
Detailed Description
The following description will further describe embodiments of the present invention with reference to the accompanying drawings and examples. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
As shown in figure 1, the utility model relates to a sampling device of a bioreactor, which comprises a sampling pipeline, an air filter 8 and thumb clamps 11-14, luer 9 and disposable syringe 10, the sampling pipeline includes being responsible for 2 and branch pipe, be responsible for 2 below and connect first silicone tube 3, be responsible for 2 top and connect second silicone tube 4, the other end submergence of first silicone tube 3 is under the liquid level of retort 1, the second silicone tube 4 that is connected above being responsible for 2 again through the adapter with third silicone tube 5, fourth silicone tube 6 link to each other, third silicone tube 5 links to each other with fifth silicone tube 7, fourth silicone tube 6 links to each other with air cleaner 8, luer 9 is connected to the end of fifth silicone tube 7, thumb clamp 11-14 presss from both sides and is used as on-off control on the silicone tube, disposable syringe 10 and luer 9 are separated, use disposable syringe 10 to draw when taking a sample. The branch pipe is connected with the branch pipe through a two-way adapter or a three-way adapter, the silicone tubes outside the reaction tank are all fastened through a binding belt, the branch pipe is a silicone branch pipe, the silicone branch pipe is a hose made of a silicone material, and the main pipe 2 is a stainless steel pipeline.
The silicone tube was 25 gauge, the air filter 8 was 0.2 μm gauge, the disposable syringe 10 was 20mL gauge, the luer 9 was 3/16 gauge, and the stainless steel tube 2 had an inner diameter of 1 cm.
When sampling, the thumb clamps 11-14 are closed, the luer connector 9 of the sampling port is opened, the disposable injector 10 is connected with the luer connector 9, negative pressure is formed in the pipeline through air suction, the thumb clamps 14, 12 and 11 are loosened slowly in sequence at the moment, cell culture fluid flows out of the reactor, after the required amount of fluid flows out, the thumb clamp 14 is clamped, the injector is pulled out, a 75% alcohol pot is used for spraying and disinfecting the sampling port, the luer connector 9 is screwed tightly, the luer connector 9 is wrapped by tin foil, then the pipeline is lifted up, the thumb clamp 13 is opened, the culture fluid in the pipeline is put back into the reactor, all the thumb clamps are closed, and sampling is completed.
This sampling device passes through the syringe extraction sample, in time closes the interface, has avoided culture solution and external environment direct contact, has reduced the risk of being polluted by external environment, has realized aseptic sample, has made things convenient for personnel to bioreactor's sample operation.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. The utility model provides a sampling device of bioreactor, its characterized in that, this sampling device of bioreactor includes sampling pipe, air cleaner, thumb clamp, luer joint and disposable syringe, sampling pipe is including being responsible for and branch pipe, be responsible for the below and connect first silicone tube, be responsible for the top and connect the second silicone tube, be responsible for the second silicone tube connection branch pipe that the top is connected, air cleaner is connected to branch pipe one end, branch pipe end-to-end connection luer joint, the thumb clamp is on the branch pipe, disposable syringe and luer joint are the separation.
2. The bioreactor sampling device as claimed in claim 1, wherein the silicone tubes outside the reaction tank are fastened by a ribbon.
3. The bioreactor sampling device as claimed in claim 2, wherein the branch pipe is a silica gel branch pipe, and the silica gel branch pipe is a hose made of silica gel material.
4. The bioreactor sampling device of claim 2, wherein the main tube is a stainless steel tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122437087.7U CN217155954U (en) | 2021-10-09 | 2021-10-09 | Sampling device of bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122437087.7U CN217155954U (en) | 2021-10-09 | 2021-10-09 | Sampling device of bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217155954U true CN217155954U (en) | 2022-08-09 |
Family
ID=82659861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122437087.7U Active CN217155954U (en) | 2021-10-09 | 2021-10-09 | Sampling device of bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217155954U (en) |
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2021
- 2021-10-09 CN CN202122437087.7U patent/CN217155954U/en active Active
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