CN215767835U

CN215767835U - Sterile sampling device

Info

Publication number: CN215767835U
Application number: CN202122299369.5U
Authority: CN
Inventors: 刘璟; 王超; 周慧兵; 俞枋
Original assignee: Jitong Life Technology Suzhou Co ltd
Current assignee: Jitong Life Technology Suzhou Co ltd
Priority date: 2021-09-23
Filing date: 2021-09-23
Publication date: 2022-02-08
Anticipated expiration: 2031-09-23

Abstract

The utility model discloses an aseptic sampling device, comprising: cultivate the jar, gaseous ejecting device, sample draw-out device and three way connection, the opening part of cultivating the jar is sealed through cultivating the cover, it is used for loading liquid to cultivate the jar, gaseous ejecting device is used for ejecting gas, sample draw-out device is used for extracting and cultivates jar interior liquid, three way connection's first interface is through first pipeline and cultivation jar intercommunication, its second interface passes through the gas outlet intercommunication of second pipeline with gaseous ejecting device, its third interface passes through the sample connection intercommunication of third pipeline with sample draw-out device, and at first pipeline, the second pipeline, all be equipped with the on-off control device who is used for switching corresponding pipeline switch on the third pipeline. The utility model can perform sterile sampling operation in a complex environment without polluting the interior of the culture tank. After sampling, no residual liquid exists in each pipeline, and repeated sampling can be performed for multiple times.

Description

Sterile sampling device

Technical Field

The utility model relates to the field of biomedical instruments, experimental instruments or life science instruments, in particular to an aseptic sampling device.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. Cell culture technology is an essential link for cloning technology, and cell culture itself is the cloning of cells. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

Non-toxicity and sterility are the primary conditions for culturing cells in vitro. During the in vitro culture of cells, the defenses against microorganisms and the detoxification of harmful substances are lost due to the lack of protection of the body's immune system. To ensure that cells can be grown in an in vitro environment, sterile working areas, good personal hygiene, sterile reagents and media, and sterile handling must be ensured.

The utility model discloses a utility model patent application number 202022496193.8 discloses an aseptic sampling device, the device include sampling bottle and top cap, have set firmly the connector on the sampling bottle, and top cap and connector cooperation are equipped with three siphunculus on the top cap, and three siphunculus are inlet pipe, discharging pipe and breather pipe respectively, are equipped with the valve on the discharging pipe, are equipped with filter equipment on the breather pipe, and the inlet pipe links to each other with external equipment. The sampling device still has residues in the reagent after sampling, and the residues are remained in the sampling bottle or the discharge tube.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides an aseptic sampling device.

In order to achieve the purpose, the technical scheme of the utility model is as follows:

the utility model discloses an aseptic sampling device, which comprises:

the opening of the culture tank is sealed by a culture tank cover, and the culture tank is used for loading liquid;

the gas pushing device is used for pushing gas out;

the sampling and extracting device is used for extracting liquid in the culture tank;

the three-way joint, three-way joint's first interface passes through first pipeline and cultivates jar intercommunication, and its second interface passes through the gas outlet intercommunication of second pipeline and gas ejecting device, and its third interface passes through the sample connection intercommunication of third pipeline and sample draw-out device, and all is equipped with the on-off control device who is used for switching corresponding pipeline switch on first pipeline, second pipeline, third pipeline.

The sterile sampling device has a simple structure, can perform sterile sampling operation in a complex environment, and does not pollute the interior of the culture tank. After sampling, no residual liquid exists in each pipeline, and repeated sampling can be performed for multiple times.

On the basis of the technical scheme, the following improvements can be made:

preferably, the gas pushing device is communicated with the second pipeline through a gas filtering device.

Adopt above-mentioned preferred scheme, guarantee to get into the gas cleanness of culture tank.

Preferably, the sampling port of the sampling and extracting device is communicated with the third pipeline through a luer connector.

Adopt above-mentioned preferred scheme, guarantee that the leakproofness of connecting is good.

Preferably, the sampling and extracting device is detachably connected with the luer connector.

By adopting the preferable scheme, the replacement of parts is facilitated. When the sampling device is idle, the sampling device can be detached and the plug can be replaced.

Preferably, one end of the first pipeline extends into the bottom of the culture tank.

By adopting the preferable scheme, smooth sampling and stable liquid falling are ensured.

Preferably, a fourth pipeline is arranged on the culture tank cover, one end of the fourth pipeline is communicated with the culture tank, and the other end of the fourth pipeline is communicated with the air filtering device.

By adopting the preferable scheme, the balance of the air pressure inside and outside the culture tank is ensured.

Preferably, the switching regulator is a tube holder.

By adopting the preferable scheme, the operation is convenient and fast, and the cost is low.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a schematic structural diagram of an aseptic sampling device provided by an embodiment of the present invention.

Fig. 2 is a partial structural schematic view of an aseptic sampling device (after sampling) provided by an embodiment of the utility model.

Wherein: 1-culture tank, 11-culture tank cover, 2-gas pushing device, 3-sampling and drawing device, 4-three-way joint, 51-first pipeline, 52-second pipeline, 53-third pipeline, 54-fourth pipeline, 61-switch regulating device on the first pipeline, 62-switch regulating device on the second pipeline, 63-switch regulating device on the third pipeline, 7-luer joint, 8-gas filtering device, 9-plug and 10-air filtering device.

Detailed Description

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The use of the ordinal terms "first," "second," "third," etc., to describe a common object merely indicate that different instances of like objects are being referred to, and are not intended to imply that the objects so described must be in a given sequence, either temporally, spatially, in ranking, or in any other manner.

Also, the expression "comprising" an element is an expression of "open" which merely means that there is a corresponding component, and should not be interpreted as excluding additional components.

For the purposes of the present invention, in some of its embodiments, as shown in fig. 1, the sterile sampling device comprises: a culture tank 1, a gas pushing device 2, a sampling and extracting device 3 and a three-way joint 4.

The opening of the culture tank 1 is sealed by a culture tank 1 cover, and the culture tank 1 is filled with liquid which can be cell mixed liquid, microorganism mixed liquid and the like needing to be in a sterile environment. The gas pushing-out means 2 is for pushing out gas, and the sampling and drawing means 3 is for drawing liquid in the culture tank 1.

The first interface of the three-way joint 4 is communicated with the culture tank 1 through a first pipeline 51, the second interface thereof is communicated with the gas outlet of the gas pushing-out device 2 through a second pipeline 52, the third interface thereof is communicated with the sampling port of the sampling and extracting device 3 through a third pipeline 53, and the first pipeline 51, the second pipeline 52 and the third pipeline 53 are all provided with switch adjusting devices for switching corresponding pipeline switches.

In some embodiments, the sampling draw-off device 3 may be, but is not limited to, a sampling syringe, which may be a screw-on syringe or other syringe configuration.

In other embodiments, the sampling and extracting device 3 may also include: containers such as an extraction mechanism and test tubes or reagent bottles that can temporarily or permanently store liquids.

The sterile sampling device has simple structure, can perform sterile sampling operation in a complex environment, and does not pollute the interior of the culture tank 1. After sampling, no residual liquid exists in each pipeline, and repeated sampling can be performed for multiple times.

In order to further optimize the working effect of the utility model, in other embodiments, the remaining features are the same, except that the gas pushing device 2 is in communication with the second pipeline 52 through a gas filtering device 8.

With the above embodiment, it has the following beneficial effects: ensuring that the gas entering the culture tank 1 is clean. After the gas pushed out by the gas pushing-out device 2 passes through the gas filtering device 8, clean gas is ensured to enter the second pipeline 52.

The gas may be air or other gas.

In some embodiments, the gas-pushing device is a screw injector.

In other embodiments, the gas pushing device may be a pump, a compressed gas device, or the like that can provide positive pressure gas.

Further, in the above embodiment, the gas pushing-out device 2 is detachably connected to the gas filtering device 8.

With the above further embodiment, it has the following beneficial effects: the replacement of parts is convenient. When the device is idle, the gas pushing device 2 can be detached and the plug 9 can be replaced. When the gas injector 2 is a screw injector, the stopper 9 may be a luer stopper having the same thread structure.

In order to further optimize the working effect of the present invention, in other embodiments, the remaining features are the same, except that the sampling port of the sampling and extracting device 3 is communicated with the third pipeline 53 through the luer 7.

With the above embodiment, it has the following beneficial effects: the good sealing performance of the connection is ensured.

In order to further optimize the working effect of the utility model, in other embodiments, the remaining features are the same, except that the sampling and extraction device 3 is detachably connected to the luer 7.

With the above embodiment, it has the following beneficial effects: the replacement of parts is convenient. When the sampling device 3 is idle, the sampling device can be detached and replaced by the plug 9. The plug 9 may be a luer plug 9.

In order to further optimize the working effect of the utility model, in other embodiments, the remaining features are the same, except that one end of the first pipe 51 extends into the bottom of the culture tank 1.

With the above embodiment, it has the following beneficial effects: ensuring smooth sampling and stable liquid falling.

In order to further optimize the effect of the present invention, in other embodiments, the remaining features are the same, except that a fourth line 54 is installed on the culture tank 1 cover, one end of the fourth line 54 is communicated with the culture tank 1, and the other end is communicated with the air filtration device 10.

With the above embodiment, it has the following beneficial effects: the balance of the air pressure inside and outside the culture tank 1 is ensured.

In order to further optimize the implementation of the utility model, in other embodiments, the remaining features are the same, except that the switching device is a tube holder.

With the above embodiment, it has the following beneficial effects: the operation is convenient and the cost is low.

The embodiment of the utility model also discloses an aseptic sampling method, which utilizes the aseptic sampling device shown in figure 1 to carry out sampling and specifically comprises the following steps:

s1: starting sampling, closing the switch adjusting device 62 on the second pipeline 52 to enable the second pipeline 52 to be in a closed state;

opening the on-off adjusting device 61 on the first pipeline 51 and the on-off adjusting device 63 on the third pipeline 53, so that the first pipeline 51 and the third pipeline 53 are in an open state;

when the sampling and extracting device 3 works, the liquid in the culture tank 1 sequentially flows through the first pipeline 51, the three-way joint 4 and the third pipeline 53 to enter the sampling and extracting device 3;

s2: closing the switching regulator 61 on the first pipeline 51 to make the first pipeline 51 in a closed state;

opening the on-off regulating device 62 on the second pipeline 52 to make the second pipeline 52 in an open state;

after the gas pushed out by the gas pushing-out device 2 is filtered by the gas filtering device 8, all the liquid remained in the three-way joint 4 and the third pipeline 53 is pressed into the sampling and extracting device 3;

s3: closing the on-off regulating device 63 on the third pipeline 53 to make the third pipeline 53 in a closed state;

opening the switching regulator 61 on the first pipeline 51 to open the first pipeline 51;

the gas pushing-out device 2 pushes out the gas, and after the gas is filtered by the gas filtering device 8, all the liquid remaining in the three-way joint 4 and the first pipeline 51 flows back into the culture tank 1.

By adopting the sterile sampling method disclosed by the utility model, sterile sampling operation can be smoothly carried out in a complex environment, residual liquid flows back into the culture tank 1 after sampling, and no residual liquid exists in other pipelines or parts.

Further, in some embodiments, the sterile sampling method further comprises the steps of:

s4: closing the switch adjusting device 61 on the first pipeline 51 and the switch adjusting device 62 on the second pipeline 52 to enable the first pipeline 51 and the second pipeline 52 to be in a closed state;

the sampling and extracting device 3 is disassembled, and the corresponding outlet is blocked by a plug 9;

the gas ejector 2 is removed and the corresponding outlet is blocked with a plug 9, as shown in fig. 2.

With the above embodiment, it has the following beneficial effects: when the device is idle, the whole tightness of the device is ensured, and the liquid in the culture tank 1 is always in an aseptic state.

The plug 9 can be a luer plug.

Further, after the sampling and extracting device 3 is detached, a proper amount of 75% sterile alcohol is sprayed in the air to be provided with the luer plug, the luer connector 7 and the air nearby, and then the luer plug 9 is in threaded connection with the luer connector 7.

In summary, the sterile sampling device of the utility model has the following beneficial effects:

first, the present invention uses a pipeline to connect the culture tank 1, the gas pushing-out device 2, the sampling and drawing device 3, and the three-way joint 4, and after sampling, there is no liquid left in the pipeline.

Secondly, the aseptic sampling device disclosed by the utility model can realize multiple times of sampling because no liquid residue exists in the pipeline after the sampling is finished.

When the culture tank 1 is loaded with the cell mixture, the sterile sampling device of the present invention can be used to sample the cells several times until the desired cell density is reached (e.g., if the culture tank is not full).

The patent documents mentioned in the background art, however, are based on the fact that a small amount of liquid remains after sampling, and a large number of dead cells remain in the residue. If a second sampling is performed, the cell mixture of the second sampling is contaminated with residues. For cell sampling, there is a large deviation if the survival rate is calculated, if there is a residue. The utility model has no residue, the calculated cell viability is the actual numerical value, and the accuracy is greatly improved.

Thirdly, the liquid transmitted by the sampling device cannot be impacted in the whole sampling process disclosed by the utility model. Especially, when the liquid is cell mixture, the impact on the cells is not generated, and the sampling is successful.

In the patent documents mentioned in the background art, the feed inlet is at the tube opening, and the cell mixture drops into the bottom of the tube from the tube opening, so that the impact on some cells is too large, and the cells may die.

Fourthly, the present invention provides a liquid remaining in the first pipe 51 after the material is taken, and the liquid is returned to the culture tank 1, thereby ensuring that no liquid remains in the pipe.

Especially when the liquid is cell mixture, the cells are always in an active state.

However, the patent documents mentioned in the background art do not treat the cell mixture in the feed pipe, and use a closing device to close the feed pipe, and then open the discharge pipe, and vent the air to discharge the reagent from the discharge pipe. The closing device is in the closed condition, and the cell mixed liquid in the discharging pipe is always in the pipeline, and the cell loses the previous growing environment, will die soon. This also means that they cannot take a second or more sample because after one sample, all cells in the feed tube are dead and even outside the device connected to the feed tube.

The above embodiments are merely illustrative of the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the content of the present invention and implement the present invention, and not to limit the scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the scope of the present invention.

Claims

1. An aseptic sampling device, comprising:

the gas pushing device is used for pushing gas out;

a sampling and extracting device for extracting liquid in the culture tank;

the three-way joint, three-way joint's first interface pass through first pipeline with cultivate jar intercommunication, its second interface pass through the second pipeline with gaseous ejecting device's gas outlet intercommunication, its third interface pass through the third pipeline with sample draw-out device's sample connection intercommunication, and all be equipped with the on-off control device who is used for switching corresponding pipeline switch on first pipeline, second pipeline, the third pipeline.

2. An aseptic sampling device according to claim 1, wherein the gas push-out device communicates with the second conduit via a gas filtration device.

3. An aseptic sampling device as defined in claim 1, wherein the sampling port of the sampling and extraction device communicates with the third conduit via a luer fitting.

4. An aseptic sampling device as defined in claim 3, wherein the sampling and extraction device is removably connected to the luer fitting.

5. An aseptic sampling device according to claim 1, wherein one end of the first conduit extends into the bottom of the culture tank.

6. An aseptic sampling device as defined in claim 1, wherein a fourth pipeline is mounted on said culture tank cap, one end of said fourth pipeline is communicated with said culture tank, and the other end thereof is communicated with an air filtering device.

7. An aseptic sampling device according to any of claims 1 to 6, wherein the switch adjustment means is a tube holder.