CN101153265B - Safe and portable liquid bacterial vaccinating device - Google Patents
Safe and portable liquid bacterial vaccinating device Download PDFInfo
- Publication number
- CN101153265B CN101153265B CN2007100710119A CN200710071011A CN101153265B CN 101153265 B CN101153265 B CN 101153265B CN 2007100710119 A CN2007100710119 A CN 2007100710119A CN 200710071011 A CN200710071011 A CN 200710071011A CN 101153265 B CN101153265 B CN 101153265B
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- Prior art keywords
- bottle
- safe
- portable liquid
- air filter
- liquid bacterial
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- 239000007788 liquid Substances 0.000 title claims description 33
- 230000001580 bacterial effect Effects 0.000 title claims description 26
- 239000002184 metal Substances 0.000 claims description 20
- 229910052751 metal Inorganic materials 0.000 claims description 20
- 238000005245 sintering Methods 0.000 claims description 19
- 230000001681 protective effect Effects 0.000 claims description 8
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 abstract description 15
- 238000009630 liquid culture Methods 0.000 abstract 3
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 238000011169 microbiological contamination Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a safe portable liquid culture inoculation device which comprises a bottle mouth (41) and a bottle body (4). A bottle cover (42) is hermetically connected with the bottle mouth (41); the bottom (61) of a run-through guide conduit (6) is seamlessly passed through the bottle cover (42) and is then arranged in the inner cavity of the bottle body (4), and the run-through guide conduit (6) arranged outside the bottle body (4) is provided with a ball valve (7). The safe portable liquid culture inoculation device of the invention can prevent the culture from secondary pollution and is suitable for the long-distance carry of the liquid culture.
Description
Technical field
The present invention relates to a kind of liquid bacterial vaccinating device, particularly a kind of portable liquid bacterial vaccinating device.
Background technology
Liquid fermentation technology has can carry out the industrialization continuous production, scale is big, output is high, fermentation period is short, the production efficiency advantages of higher, by the industry of original microbiotic extend applied to medicinal and the food fungi fermentation in, thereby liquid fermentation technology is an inevitable development trend.During the fermentation, will bring serious harm to generation, the demanding liquid submerged fermentation of especially aseptic degree if infected assorted bacterium; Therefore during the fermentation, antipollution work importance is more outstanding.If device structure and duct arrangement are unreasonable or during the fermentation careless manipulation will make the fermented liquid pollution microbes, cause output to descend even can not get product.Particularly earlier fermentation microbiological contamination, assorted bacterium can consume nutritive substance very soon and cause produce bacterium can't normal growth and cause tank switching.According to the literature, both at home and abroad antibiotic fermentation is owing to the microbiological contamination of inoculation reason accounts for about 11% of whole microbiological contamination rate, and consequence can't save often, and gets only tank switching.The main method of liquid fermenting inoculation is flame inoculation and differential pressure inoculation; The advantage of flame inoculation is not need the equipment input, but quite high to operator's requirement, has carelessness just to be easy to microbiological contamination slightly, and the application in the demanding liquid fermenting of aseptic degree is produced is fewer and feweri; The differential pressure inoculation is more and more adopted modes, but inoculating facility is simple and crude, and inoculation personnel's personnel safety can't ensure, and cause the bacterial classification secondary pollution easily.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of safe and portable liquid bacterial vaccinating device that can prevent that bacterial classification is secondary polluted and the long distance of suitable liquid spawn is carried.
In order to solve the problems of the technologies described above, the invention provides a kind of safe and portable liquid bacterial vaccinating device, comprise the bottle that has bottleneck, bottle cap links to each other with the bottleneck sealing, the inner chamber that is positioned at bottle behind the bottle cap is seamlessly passed in the bottom that connects conduction pipe, ball valve is set being positioned on the perforation conduction pipe of bottle outside.
Improvement as safe and portable liquid bacterial vaccinating device of the present invention: the intracavity inter-connection with bottle is seamlessly passed behind the bottle cap in the bottom of metal sintering pipe air filter; Be provided with vacuum breaker being positioned on the metal sintering pipe air filter of bottle outside.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: on bottle, be provided with and be with graduated glass visor.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: connect the bottom of the bottom of conduction pipe near bottle intracavity.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: the top of metal sintering pipe air filter is provided with protective sleeve.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: the top that connects conduction pipe is provided with tin hat.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: be used to protect the outer jointing jacket that connects conduction pipe and metal sintering pipe air filter to link to each other with bottle cap.
Further improvement as safe and portable liquid bacterial vaccinating device of the present invention: bottle is made by pressure-resistant material.
Safe and portable liquid bacterial vaccinating device of the present invention, be applicable to the inoculation of liquid fermentation and culture, particularly be fit to demanding fungi of aseptic degree or cell inoculation, characteristics with secondary pollution of simple to operate, practical safety and minimizing bacterial classification, and easy to carry, be beneficial to the long-distance transportation and the preservation of bacterial classification, realized the possibility of the strange land production of hybrid seeds, reduce the chance that the middle-chain bacterial classification pollutes, can also ensure inoculation personnel's personnel safety.
Description of drawings
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in further detail.
Fig. 1 be safe and portable liquid bacterial vaccinating device of the present invention partly cut open structural representation.
Embodiment
A kind of safe and portable liquid bacterial vaccinating device comprises the bottle 4 that has bottleneck 41, and this bottle 4 is made by pressure-resistant material, for example can adopt the metallic substance titanium to make, so this bottle 4 has the advantage of easy to carry, light and handy and withstand voltage and acid and alkali-resistance.On bottle 4, be provided with and be with graduated glass visor 5, can find out the inner chamber of bottle 4 by this glass visor 5.On bottleneck 41, be provided with the screw thread that is used to connect, be provided with the internal thread that matches with above-mentioned screw thread at the inwall of bottle cap 42; Rely on the connection of screw thread, bottleneck 41 is realized linking to each other with the sealing of bottle cap 42.
The inner chamber that is positioned at bottle 4 behind the bottle cap 42 is seamlessly passed in the bottom 61 that connects conduction pipe 6, and this bottom 61 is near the bottom of bottle 4 inner chambers.Therefore, following more than half section (i.e. this section of close bottom 61) connecting conduction pipe 6 is positioned at 4 inner chambers of bottle, and the last smaller part section that connects conduction pipe 6 is positioned at the outside of bottle 4.Connect conduction pipe 6 on smaller part section place ball valve 7 is set, this ball valve 7 be used to control connect conduction pipe 6 conducting whether.
Be provided with tin hat 8 on the top that connects conduction pipe 6, being connected between tin hat 8 and the top that connects conduction pipe 6 is threaded, and tin hat 8 is used to protect the top of perforation conduction pipe 6 not to be subjected to outside contamination.
The intracavity inter-connection of bottle cap 42 backs and bottle 4 is seamlessly passed in the bottom 21 of metal sintering pipe air filter 2; The bottom 21 of metal sintering pipe air filter 2 is near the internal surface of bottle cap 42.Be provided with vacuum breaker 3 being positioned on the metal sintering pipe air filter 2 of bottle 4 outsides, this vacuum breaker 3 plays the effect of check valve, can prevent in transportation liquid from the inner chamber of bottle 4 to external leakage.Top at metal sintering pipe air filter 2 is provided with protective sleeve 1, and being connected between the top of protective sleeve 1 and metal sintering pipe air filter 2 is threaded, and protective sleeve 1 is used to protect the top of metal sintered pipes air filter 2 not to be subjected to outside contamination.The effect of metal sintering pipe air filter 2 is to remove the airborne assorted bacterium that enters in bottle 4 chambeies.
Outer wall at bottle cap 42 is provided with outside screw; Outer jointing jacket 9 is provided with the screw thread that matches with this outside screw, relies on the connection of screw thread, and outer jointing jacket 9 is realized linking to each other with bottle cap 42.At this moment, connect conduction pipe 6 and metal sintering pipe air filter 2 and all be positioned at outer jointing jacket 9, accept the protection of outer jointing jacket 9, be used for preventing the generation of actual transportation unscheduled event.
Safe and portable liquid bacterial vaccinating device of the present invention, actual use is as follows:
1, takes off protective sleeve 1 and outer jointing jacket 9, then whole classification inoculation apparatus is put into high-pressure steam sterilizing pan and carry out disinfection.In the clean platform of behaviour, have under the condition of flame protection again, cooled bottle cap 42 and bottleneck 41 are separated.Then liquid spawn is put into the inner chamber of bottle 4, then bottle cap 42 is fixed on the bottleneck 41.Put protective sleeve 1 and outer jointing jacket 9 at last successively, close ball valve 7 again.
2, when the above-mentioned classification inoculation apparatus that liquid spawn is housed being transported to the point of destination need inoculating, take off outer jointing jacket 9 and tin hat 8.A wherein end of aseptic rubber hose is connected with the top that connects thrust-augmenting nozzle 6; The mouth of pipe of the other end of aseptic rubber hose and fermentor tank inoculated tube joins, and fixes with clip.Take off protective sleeve 1 again, rubber hose one end is linked to each other with the compressed air outlet pipe, the top of the rubber hose the other end and metal sintering pipe air filter 2 joins, and fixes with clip.Open the valve on ball valve 7 and the fermentor tank inoculated tube, slowly open compressed air control valve, air flows into through the top of metal sintering pipe air filter 2, after the 2 inner filtration degerming of metal sintering pipe air filter, flow out from the bottom 21 of metal sintering pipe air filter 2, enter in the bottle 4.Under the effect of pressure, the liquid spawn in the bottle 4 enters successively by the bottom 61 that connects thrust-augmenting nozzle 6 and connects thrust-augmenting nozzle 6 and fermentor tank inoculated tube, finally slowly is pressed in the fermentor tank.
Connecing bacterium amount can observe by being with graduated glass visor 5, reach connect the bacterium amount after, first closes compression air control valve is closed the valve on ball valve 7 and the fermentor tank inoculated tube again; Take off afterwards and connect the aseptic rubber hose that thrust-augmenting nozzle 6 links to each other and just finish inoculation step.
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (4)
1. safe and portable liquid bacterial vaccinating device, it is characterized in that: comprise the bottle (4) that has bottleneck (41), bottle cap (42) links to each other with bottleneck (41) sealing, the inner chamber that is positioned at bottle (4) behind the bottle cap (42) is seamlessly passed in the bottom (61) that connects conduction pipe (6), on the perforation conduction pipe (6) that is positioned at bottle (4) outside ball valve (7) is set, the top that connects conduction pipe (6) is provided with tin hat (8); The intracavity inter-connection of bottle cap (42) back and bottle (4) is seamlessly passed in the bottom (21) of metal sintering pipe air filter (2); Be provided with vacuum breaker (3) on the metal sintering pipe air filter (2) that is positioned at bottle (4) outside, the top of metal sintering pipe air filter (2) is provided with protective sleeve (1); The outer jointing jacket (9) that is used for protection perforation conduction pipe (6) and metal sintering pipe air filter (2) links to each other with bottle cap (42).
2. safe and portable liquid bacterial vaccinating device according to claim 1 is characterized in that: be provided with on bottle (4) and be with graduated glass visor (5).
3. safe and portable liquid bacterial vaccinating device according to claim 2 is characterized in that: connect the bottom of the bottom (61) of conduction pipe (6) near bottle (4) inner chamber.
4. safe and portable liquid bacterial vaccinating device according to claim 3 is characterized in that: described bottle (4) is made by pressure-resistant material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100710119A CN101153265B (en) | 2007-08-29 | 2007-08-29 | Safe and portable liquid bacterial vaccinating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100710119A CN101153265B (en) | 2007-08-29 | 2007-08-29 | Safe and portable liquid bacterial vaccinating device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101153265A CN101153265A (en) | 2008-04-02 |
| CN101153265B true CN101153265B (en) | 2010-11-17 |
Family
ID=39255150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100710119A Expired - Fee Related CN101153265B (en) | 2007-08-29 | 2007-08-29 | Safe and portable liquid bacterial vaccinating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101153265B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518534A (en) * | 2013-10-15 | 2014-01-22 | 甘肃省科学院生物研究所 | Edible fungus liquid strain storing and inoculating integrated machine |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244435B (en) * | 2016-09-27 | 2018-09-28 | 新疆惠森生物技术有限公司 | A kind of classification inoculation apparatus and its manufacturing method for microbial manure liquid fermentation |
| CN106244434B (en) * | 2016-09-27 | 2018-09-28 | 新疆惠森生物技术有限公司 | It is a kind of suitable for the classification inoculation apparatus of microbial manure liquid fermentation and making and application |
-
2007
- 2007-08-29 CN CN2007100710119A patent/CN101153265B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518534A (en) * | 2013-10-15 | 2014-01-22 | 甘肃省科学院生物研究所 | Edible fungus liquid strain storing and inoculating integrated machine |
| CN103518534B (en) * | 2013-10-15 | 2015-03-18 | 甘肃省科学院生物研究所 | Edible fungus liquid strain storing and inoculating integrated machine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101153265A (en) | 2008-04-02 |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
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| CP01 | Change in the name or title of a patent holder |
Address after: 310027 Hangzhou, Zhejiang Province, Xihu District, Zhejiang Road, No. 38, No. Co-patentee after: Zhejiang Dayang Chemical Co., Ltd. Patentee after: Zhejiang University Address before: 310027 Hangzhou, Zhejiang Province, Xihu District, Zhejiang Road, No. 38, No. Co-patentee before: Jiande Dayang Chemical Co., Ltd. Patentee before: Zhejiang University |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101117 Termination date: 20140829 |
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| EXPY | Termination of patent right or utility model |