CN208768679U - A kind of plant tissue culture reaction tank - Google Patents

Abstract

The utility model discloses a kind of plant tissue culture reactor tanks, including liquid storage bottle (1) and the culture tank on the liquid storage bottle (1) top is set, the culture tank includes the lid (3) of Culture basin (2) and setting on the Culture basin (2) top, the liquid in-out mouth (4) being connected with the inside of the Culture basin (2) is provided in the lower part of the Culture basin (2), the lower end of the liquid in-out mouth (4) is provided with first interface (5), the top of the lid (3) is provided with second interface (6) and third interface (7), culture solution injection port (8) are provided on a side of the liquid storage bottle (1), 4th interface (9), 5th interface (10) and the 6th interface (11).Plant tissue culture described in the utility model reaction jar structure is simple, easy to operate, sealing effect is good, be convenient for changing culture medium, later maintenance cost is low.

Description

A kind of plant tissue culture reactor tank

Technical field

The utility model relates to field of plant tissue culture, more particularly to a kind of plant tissue culture reactor tank.

Background technique

The tissue cultures of plant are that have this theory of totipotency, one to grow up in recent decades according to plant cell The new technology of item vegetative propagation.The tissue cultures of plant are called in vitro culture, refer to isolated from plant the tissue to suit the requirements, Organ or cell, protoplast etc. are aseptically seeded in and are swashed containing various nutriments and plant by sterile working It is cultivated on the culture medium of element to obtain the technology of other products of regenerated intact plant or production with economic value.

Although existing tissue culture reactor tank can reach identical efficiency when being inoculated with, poorly sealed, problem is more serious, also Although some similar products sealing problems are solved, seal operation is cumbersome, is easy to lead in the presence of replacement culture medium operation The problem of causing pollution.

Utility model content

That the technical problem to be solved by the present invention is to provide a kind of structures is simple, easy to operate, sealing effect is good, the later period Maintenance cost is low, is convenient for changing the plant tissue culture reactor tank of culture medium.

A kind of plant tissue culture reactor tank, including liquid storage bottle and the culture tank that the liquid storage bottle top is arranged in, the culture Tank includes Culture basin and the lid that the Culture basin top is arranged in, and is provided with and the Culture basin in the lower part of the Culture basin The liquid in-out mouth that is connected of inside, the lower end of the liquid in-out mouth is provided with first interface, is set on the top of the lid It is equipped with second interface and third interface, culture solution injection port, the 4th interface, are provided on a side of the liquid storage bottle The top of the 6th interface, the 6th interface is arranged in five interfaces and the 6th interface, the 4th interface and the 5th interface It is connected with the first interface.

Plant tissue culture reactor tank described in the utility model, wherein the Culture basin and the lid pass through retaining mechanism It is fixed, silica gel sealing ring is provided between the Culture basin and the lid.

Plant tissue culture reactor tank described in the utility model, wherein the vertical section of the silica gel sealing ring is U-shaped, institute Stating retaining mechanism is ring-shaped clip.

Plant tissue culture reactor tank described in the utility model, wherein the first interface, the second interface, described Three interfaces, the 4th interface, the 5th interface and the 6th interface are female Luer, and the culture solution injection port is GL45 bottleneck.

Plant tissue culture reactor tank described in the utility model, wherein the liquid storage bottle is rectangular parallelepiped structure, the liquid storage bottle For storing plant tissue culture fluid nutrient medium.

Plant tissue culture reactor tank described in the utility model, wherein the second interface and the third interface and air Filter is connected.

Plant tissue culture reactor tank described in the utility model, wherein circular groove is provided at the top of the lid, In the groove, hand grip portion is set.

Plant tissue culture reactor tank described in the utility model, wherein the bottom of the inside of the Culture basin is provided with tool There is the arc convex of guide functions.

Plant tissue culture reactor tank described in the utility model, wherein be removable between the culture tank and the liquid storage bottle It connects with unloading.

The utility model plant tissue culture reactor tank difference from prior art is:

Culture tank in the utility model plant tissue culture reactor tank is opened and closing is simple, efficient；Ring-shaped clip and U-shaped silicon Glue sealing ring, sealing are tight, it is not easy to which gas leakage causes to pollute；Culture tank is connected with liquid storage bottle by female Luer hose, is convenient for Plant late stage of culture replaces culture medium, safe ready.Total interface in the utility model is international standard interface, later period dimension It protects low in cost.

The plant tissue culture reactor tank of the utility model is described further with reference to the accompanying drawing.

Detailed description of the invention

Fig. 1 is the schematic perspective view of the utility model plant tissue culture reactor tank；

Fig. 2 is schematic perspective view of the utility model plant tissue culture reactor tank under another visual angle；

Fig. 3 is the structural schematic diagram of Culture basin in the utility model plant tissue culture reactor tank.

Specific embodiment

As shown in FIG. 1 to 3, the utility model plant tissue culture reactor tank includes liquid storage bottle 1 and is arranged on 1 top of liquid storage bottle Culture tank, culture tank includes Culture basin 2 and the lid 3 that 2 top of Culture basin is arranged in, the lower part of Culture basin 2 be provided with The liquid in-out mouth 4 that the inside of Culture basin 2 is connected is provided with first interface 5 in the lower end of liquid in-out mouth 4, on the top of lid 3 It is provided with second interface 6 and third interface 7, is provided with culture solution injection port 8, the 4th interface on a side of liquid storage bottle 1 9, the top of the 6th interface 11, the 6th interface is arranged in the 5th interface 10 and the 6th interface 11, the 4th interface 9 and the 5th interface 10 11 are connected with first interface 5.

The following are optimal technical schemes:

Culture basin 2 and lid 3 are fixed by retaining mechanism 12, are provided with silica gel sealing between Culture basin 2 and lid 3 Circle, the vertical section of silica gel sealing ring be it is U-shaped, retaining mechanism 12 is ring-shaped clip.

First interface 5, second interface 6, third interface 7, the 4th interface 9, the 5th interface 10 and the 6th interface 11 are Shandong That interface, culture solution injection port 8 are GL45 bottleneck.

Liquid storage bottle 1 is rectangular parallelepiped structure, and fluid nutrient medium, second interface 6 and third interface 7 are provided in liquid storage bottle 1 It is connected with air filter.

The top of lid 3 is provided with circular groove, hand grip portion 13 is set in groove, in the inside of Culture basin 2 Bottom is provided with the arc convex 14 with guide functions, is more advantageous to the recycling of culture solution.It is between culture tank and liquid storage bottle 1 It is detachably connected, it is easy to use and cleans.

The course of work of the utility model plant tissue culture reactor tank:

Before use, second interface 6 and the 4th interface 9 are separately connected an air inlet pipe, air inlet pipe connects air pump, and third connects Mouth 7 and the 5th interface 10 are connected with escape pipe, are provided with valve in each air inlet pipe and an air outlet pipe.

When cultivating plant, the outlet tube valve that the air inlet pipe valve and third interface 7 for opening the connection of the 4th interface 9 connect, Air pump work inputs gas, and the culture solution in liquid storage bottle 1 passes through the 6th interface 11 and first interface 5 under the action of gas pressure The pipeline of connection enters in Culture basin 2, and gas is discharged by third interface 7.After air inlet, air intake valve is closed, at one section Impregnate plant tissue in liquid medium.

When recycling culture solution, the outlet pipe valve of air inlet pipe valve and the connection of the 5th interface 10 that second interface 6 connects is opened Door, air pump work input gas, is pushed back fluid nutrient medium in liquid storage bottle 1 by pressure, gas is discharged by the 5th interface 10.

In no gas and culture solution cycle operation, Liquid Culture of the 5th interface 10 into liquid storage bottle 1 can be passed through Hormone or nutritional ingredient are added in base.

Wherein, the gas being related to provides equipment and control mode and can use and function arbitrarily may be implemented in the prior art The control system specially designed also can be used in equipment and mode, not within the protection scope of the present utility model.

Embodiment described above is only that preferred embodiments of the present invention are described, not practical to this Novel range is defined, and under the premise of not departing from the spirit of the design of the utility model, those of ordinary skill in the art are to this The various changes and improvements that the technical solution of utility model is made should all fall into the protection that the utility model claims book determines In range.

Claims (9)

1. a plant tissue culture reaction tank, is characterized in that: comprise liquid storage bottle (1) and be arranged on the cultivation tank of described liquid storage bottle (1) top, described cultivation tank comprises cultivation basin (2) and is arranged on The cover body (3) on the upper part of the culture basin (2) is provided with a liquid inlet and outlet (4) communicated with the interior of the culture basin (2) at the lower part of the culture basin (2). The lower end of the liquid inlet and outlet (4) is provided with a first interface (5), the upper part of the cover body (3) is provided with a second interface (6) and a third interface (7), and the liquid storage bottle ( 1) One side surface is provided with a culture fluid inlet (8), a fourth interface (9), a fifth interface (10) and a sixth interface (11), the fourth interface (9) and the fifth interface (10) is arranged on the upper part of the sixth interface (11), and the sixth interface (11) is connected with the first interface (5). 2. plant tissue culture reaction tank according to claim 1, is characterized in that: described cultivation basin (2) and described cover body (3) are fixed by locking mechanism (12), in described cultivation basin (2) ) and the cover body (3) is provided with a silicone sealing ring. 3. The plant tissue culture reaction tank according to claim 2, characterized in that: the vertical section of the silicone sealing ring is U-shaped, and the locking mechanism (12) is an annular clamp. 4. The plant tissue culture reaction tank according to claim 1, characterized in that: the first interface (5), the second interface (6), the third interface (7), the fourth interface The interface (9), the fifth interface (10) and the sixth interface (11) are all Luer interfaces, and the culture fluid injection port (8) is a GL45 bottle mouth. 5. The plant tissue culture reaction tank according to claim 1, characterized in that: the liquid storage bottle (1) has a cuboid structure, and the liquid storage bottle (1) is used for storing liquid medium for plant tissue culture. 6. The plant tissue culture reaction tank according to claim 4, wherein the second interface (6) and the third interface (7) are connected with an air filter. 7. The plant tissue culture reaction tank according to claim 1 is characterized in that: a circular groove is provided on the top of the cover body (3), and a grip portion (13) is provided at the groove. . 8 . The plant tissue culture reaction tank according to claim 2 , wherein an arc-shaped protrusion ( 14 ) with a diversion effect is arranged at the bottom of the inside of the cultivation pot ( 2 ). 9 . 9 . The plant tissue culture reaction tank according to claim 8 , wherein a detachable connection is made between the culture tank and the liquid storage bottle ( 1 ). 10 .