CN201176433Y - Apparatus preparing anaerobic culture fluid - Google Patents
Apparatus preparing anaerobic culture fluid Download PDFInfo
- Publication number
- CN201176433Y CN201176433Y CNU2008200894146U CN200820089414U CN201176433Y CN 201176433 Y CN201176433 Y CN 201176433Y CN U2008200894146 U CNU2008200894146 U CN U2008200894146U CN 200820089414 U CN200820089414 U CN 200820089414U CN 201176433 Y CN201176433 Y CN 201176433Y
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- gas circuit
- circuit pipe
- steel cylinder
- culture fluid
- threeway
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Abstract
The utility model relates to a device for preparing anaerobic culture fluid, which relates to a cell culture device. The device aims at solving the problems that the anaerobic environment of an existing culture dish is insufficient, which is not beneficial to the cell growth, and anaerobic culture fluid which meets the requirements can not be prepared. A nitrogen steel cylinder, a nitrogen and carbon dioxide compound steel cylinder or a hydrogen gas and carbon dioxide compound steel cylinder is connected with a first air passage pipe, wherein a second air passage pipe is communicated with a left side port on a three-mouth flask, an extractor is arranged on a sucking port on the three-mouth flask, a heater is arranged under the three-mouth flask, a third air passage pipe is connected with a vacuum air pump, an air distributor is arranged on a charging port on the third air passage pipe, an anaerobic charging pipeline is connected with the charging port and is communicated with a culture fluid bottle, a flexible pipe is connected with the extractor and is communicated with the culture fluid battle. The device can fully form an anaerobic environment in the spaces in the three-mouth flask and the culture fluid bottle, and is beneficial to the cell growth, thereby the anaerobic culture fluid meeting the requirements can be prepared.
Description
Technical field
The utility model relates to a kind of yeast culture device.
Background technology
Existing technology generally is to use dull and stereotyped culture dish to carry out inoculum preparation, nutrient solution (solid or liquid) is put into the culture dish flat board, thalline is inserted wherein, but this method can't be created anaerobic environment, be only applicable to aerobic bacteria or the cultivation of the undemanding thalline of oxygen requirement; Simultaneously, the anaerobism thalline of domestic present research is not strict especially to the requirement of oxygen, generally uses CO2gas incubator just passable, and general put polytetrafluoroethylene film on bottle, adds gum cover and fixes and get final product.Its common drawback is that the anaerobic environment of creating is insufficient, is unfavorable for the growth of thalline.And complicated operating process is loaded down with trivial details, is unfavorable for large batch of production nutrient solution.
The utility model content
The purpose of this utility model is insufficient for the anaerobic environment that solves existing culture dish, is unfavorable for the growth of thalline, can not prepare the problem of satisfactory anaerobism nutrient solution, and a kind of anaerobism inoculum preparation device is provided.
The utility model comprises the nitrogen steel cylinder, nitrogen and carbon dioxide mixture steel cylinder or hydrogen and carbon dioxide mixture steel cylinder 1, first reducing valve 4, the first gas circuit pipe 5, condenser 6, there-necked flask 7, well heater 8, extractor 9, gas distributor 10, anaerobism loading line 11, culture jar 12, vacuum pump 13, flexible pipe 15, first threeway 16, the second gas circuit pipe 19, the 3rd gas circuit pipe 21 and second reducing valve 22, the nitrogen steel cylinder, nitrogen is connected by the end of reducing valve 4 with the first gas circuit pipe 5 with carbon dioxide mixture steel cylinder 1 with carbon dioxide mixture steel cylinder or hydrogen, the other end of the first gas circuit pipe 5 is connected with first threeway 16, one end of the second gas circuit pipe 19 is connected with first threeway 16, the other end of the second gas circuit pipe 19 communicates with left side mouth 72 on the there-necked flask 7, middle port 71 on the there-necked flask 7 is connected with condenser 6, pump orifice 73 on the there-necked flask 7 is provided with extractor 9, well heater 8 be arranged on there-necked flask 7 below, one end of the 3rd gas circuit pipe 21 is connected with first threeway 16, the other end of the 3rd gas circuit pipe 21 is connected with vacuum pump 13 by second reducing valve 22, the 3rd gas circuit pipe 21 is provided with inflation inlet 20, inflation inlet 20 is provided with gas distributor 10, one end of anaerobism loading line 11 is connected with inflation inlet 20, the other end of anaerobism loading line 11 communicates with culture jar 12, one end of flexible pipe 15 is connected with extractor 9, and the other end of flexible pipe 15 communicates with culture jar 12.
The utility model has the advantages that: one, the utility model is to utilize the first gas circuit pipe 5 to charge into nitrogen (N in there-necked flask 7 and culture jar 12
2), nitrogen and carbon dioxide mixture (N
2/ CO
2) or hydrogen and carbon dioxide mixture (H
2/ CO
2), oxygen is all discharged, anaerobic environment is caused in the spaces in there-necked flask 7 and the culture jar 12 fully, helps the growth of thalline, thereby prepares satisfactory anaerobism nutrient solution.Two, the utility model is simple in structure, cost is low, the convenient use.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present utility model.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1, present embodiment is by the nitrogen steel cylinder, nitrogen and carbon dioxide mixture steel cylinder or hydrogen and carbon dioxide mixture steel cylinder 1, first reducing valve 4, the first gas circuit pipe 5, condenser 6, there-necked flask 7, well heater 8, extractor 9, gas distributor 10, anaerobism loading line 11, culture jar 12, vacuum pump 13, flexible pipe 15, first threeway 16, the second gas circuit pipe 19, the 3rd gas circuit pipe 21 and second reducing valve 22 are formed, the nitrogen steel cylinder, nitrogen is connected by the end of reducing valve 4 with the first gas circuit pipe 5 with carbon dioxide mixture steel cylinder 1 with carbon dioxide mixture steel cylinder or hydrogen, the other end of the first gas circuit pipe 5 is connected with first threeway 16, one end of the second gas circuit pipe 19 is connected with first threeway 16, the other end of the second gas circuit pipe 19 communicates with left side mouth 72 on the there-necked flask 7, middle port 71 on the there-necked flask 7 is connected with condenser 6, pump orifice 73 on the there-necked flask 7 is provided with extractor 9, well heater 8 be arranged on there-necked flask 7 below, one end of the 3rd gas circuit pipe 21 is connected with first threeway 16, the other end of the 3rd gas circuit pipe 21 is connected with vacuum pump 13 by second reducing valve 22, the 3rd gas circuit pipe 21 is provided with inflation inlet 20, inflation inlet 20 is provided with gas distributor 10, one end of anaerobism loading line 11 is connected with inflation inlet 20, the other end of anaerobism loading line 11 communicates with culture jar 12, one end of flexible pipe 15 is connected with extractor 9, and the other end of flexible pipe 15 communicates with culture jar 12.Condenser 6 makes recycling after the condensation of gas that enters in the middle port 71, and the quantity of culture jar 12 quantity of nutrient solution as required increases or reduces; Vacuum pump 13 is used for gas circuit is carried out back suction, gets rid of residual gas.
Embodiment two: present embodiment is described in conjunction with Fig. 1, the difference of present embodiment and embodiment one is that it also increases tensimeter 14, second threeway 17, pipeline 18 and the 3rd reducing valve 23 are arranged, the 3rd gas circuit pipe 21 between gas distributor 10 and the vacuum pump 13 is provided with second threeway 17, one end of pipeline 18 is connected with second threeway 17, and the other end of pipeline 18 is connected with tensimeter 14 by the 3rd reducing valve 23.Tensimeter 14 is used for monitoring the pressure in the first gas circuit pipe 5, and the pressure of avoiding entering in the first gas circuit pipe 5 is not enough to for a short time with aerification in each culture jar 12.Other composition and annexation are identical with embodiment one.
Principle of work: nutrient solution (solid or liquid) is put into there-necked flask 7, gas in nitrogen steel cylinder, nitrogen and carbon dioxide mixture steel cylinder or hydrogen and the carbon dioxide mixture steel cylinder 1 is entered the first gas circuit pipe 5 by reducing valve 4, gas circuit is divided into two-way, leading up to first threeway 16 and the 3rd gas circuit pipe 21 enters in the culture jar 12 through anaerobism loading lines 11, oxygen in the culture jar 12 is discharged, create anaerobic environment; Another road enters in the there-necked flask 7 by first threeway 16 and the second gas circuit pipe 19, lighting well heater 8 makes nutrient solution (solid or the liquid) dissolving in the there-necked flask 7 create anaerobic environment simultaneously, drawing quantitative culture liquid with extractor 9 changes in the culture jar 12 by flexible pipe 15, the culture jar 12 sealed high pressures sterilization that nutrient solution is housed is standby, finish the preparation of anaerobism nutrient solution this moment.
Claims (2)
1, a kind of anaerobism inoculum preparation device, it comprises the nitrogen steel cylinder, nitrogen and carbon dioxide mixture steel cylinder or hydrogen and carbon dioxide mixture steel cylinder (1), first reducing valve (4), the first gas circuit pipe (5), condenser (6), there-necked flask (7), well heater (8), extractor (9), gas distributor (10), anaerobism loading line (11), culture jar (12), vacuum pump (13), flexible pipe (15), first threeway (16), the second gas circuit pipe (19), the 3rd gas circuit pipe (21) and second reducing valve (22), it is characterized in that the nitrogen steel cylinder, nitrogen is connected by the end of reducing valve (4) with the first gas circuit pipe (5) with carbon dioxide mixture steel cylinder (1) with carbon dioxide mixture steel cylinder or hydrogen, the other end of the first gas circuit pipe (5) is connected with first threeway (16), one end of the second gas circuit pipe (19) is connected with first threeway (16), the other end of the second gas circuit pipe (19) communicates with left side mouth (72) on the there-necked flask (7), middle port (71) on the there-necked flask (7) is connected with condenser (6), pump orifice (73) on the there-necked flask (7) is provided with extractor (9), well heater (8) be arranged on there-necked flask (7) below, one end of the 3rd gas circuit pipe (21) is connected with first threeway (16), the other end of the 3rd gas circuit pipe (21) is connected with vacuum pump (13) by second reducing valve (22), the 3rd gas circuit pipe (21) is provided with inflation inlet (20), inflation inlet (20) is provided with gas distributor (10), one end of anaerobism loading line (11) is connected with inflation inlet (20), the other end of anaerobism loading line (11) communicates with culture jar (12), one end of flexible pipe (15) is connected with extractor (9), and the other end of flexible pipe (15) communicates with culture jar (12).
2, according to the described anaerobism inoculum preparation of claim 1 device, it is characterized in that it also includes tensimeter (14), second threeway (17), pipeline (18) and the 3rd reducing valve (23), the 3rd gas circuit pipe (21) between gas distributor (10) and the vacuum pump (13) is provided with second threeway (17), one end of pipeline (18) is connected with second threeway (17), and the other end of pipeline (18) is connected with tensimeter (14) by the 3rd reducing valve (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200894146U CN201176433Y (en) | 2008-03-10 | 2008-03-10 | Apparatus preparing anaerobic culture fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200894146U CN201176433Y (en) | 2008-03-10 | 2008-03-10 | Apparatus preparing anaerobic culture fluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201176433Y true CN201176433Y (en) | 2009-01-07 |
Family
ID=40216449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200894146U Expired - Fee Related CN201176433Y (en) | 2008-03-10 | 2008-03-10 | Apparatus preparing anaerobic culture fluid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201176433Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106222083A (en) * | 2016-08-18 | 2016-12-14 | 袁宇志 | Intelligent pair of gas culture tank control system |
| CN107164228A (en) * | 2017-05-12 | 2017-09-15 | 杨健 | CO2gas incubator |
| CN107175242A (en) * | 2017-07-07 | 2017-09-19 | 湖南大学 | Anaerobic culturel bottle inflation vacuumizes purging system |
-
2008
- 2008-03-10 CN CNU2008200894146U patent/CN201176433Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106222083A (en) * | 2016-08-18 | 2016-12-14 | 袁宇志 | Intelligent pair of gas culture tank control system |
| CN107164228A (en) * | 2017-05-12 | 2017-09-15 | 杨健 | CO2gas incubator |
| CN107175242A (en) * | 2017-07-07 | 2017-09-19 | 湖南大学 | Anaerobic culturel bottle inflation vacuumizes purging system |
| CN107175242B (en) * | 2017-07-07 | 2019-12-13 | 湖南大学 | Anaerobic culture bottle inflation vacuumizing cleaning system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090107 Termination date: 20100310 |