CN201915101U - Bioreactor for animal cell culture - Google Patents
Bioreactor for animal cell culture Download PDFInfo
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- CN201915101U CN201915101U CN2010206686657U CN201020668665U CN201915101U CN 201915101 U CN201915101 U CN 201915101U CN 2010206686657 U CN2010206686657 U CN 2010206686657U CN 201020668665 U CN201020668665 U CN 201020668665U CN 201915101 U CN201915101 U CN 201915101U
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Abstract
The utility model provides a bioreactor for animal cell culture and solves the problems in the prior art that the bioreactor for animal cell culture fails to maintain a low shearing force and reduce the damage on animal cells while ensuring a good mass transfer effect. The bioreactor for animal cell culture comprises a tank body, a gas inlet pipe, solenoid valves, a ventilation component, an exhaust component, a detection component and a switch control component, wherein the ventilation component comprises a surface ventilation pipe and a deep ventilation pipe, the detection component is used for detecting ambient parameters of the tank body to obtain ambient parameter detected values, and the switch control component is used for controlling the opening or closing of the first solenoid valve and the second solenoid valve according to the difference of the ambient parameter detected values and the preset values. The bioreactor for animal cell culture ensures good mass transfer effect and increases the oxygen transfer coefficient, thereby achieving good effect of controlling the environment of the bioreactor. Besides, the bioreactor for animal cell culture maintains a low shearing force and reduces damage on the animal cells caused by the breakup of bubbles.
Description
Technical field
The utility model relates to the bioreaction technology field, particularly a kind of animal cell culture bioreactor.
Background technology
Carry out in the animal cell large-scale culturing process at the applying biological reactor, ventilation, exhaust directly influence the factors such as gas-liquid mass transfer, biomass, meta-bolites amount and cell injury of animal cell culture bioreactor.On the one hand, feeding gas is cell growth and the required dissolved oxygen of metabolism source, the important environmental parameters of adjustable zooblast growth, suitable simultaneously ventilation can keep good mixing state and cell dispersiveness, the bubble of ventilation formation breaks and can produce shearing force on the other hand, shearing force can cause cell injury, influences cellular form, state of aggregation, metabolism and cell survival rate etc.The dual character of gas effect has caused the complicacy of animal cell culture and control, makes gas system become animal cell culture bioreactor design, optimization and engineering emphasis and the difficult point in amplifying.
The gas system of animal cell culture bioreactor gas to be processed comprises oxygen, carbonic acid gas, pressurized air and nitrogen etc., and the major function of these gases comprises the controlling tank internal pressure, oxygen is provided, controls oxygen dissolving value and regulates potential of hydrogen etc.
At present, the stirring-type animal cell culture bioreactor is used the most general in the industrialization animal cell culture, the single ventilating mode that it adopts gas directly to blast usually, as ventilation of deep layer bubbling or cage ventilation etc., single ventilating mode can not be taken into account the dual character of gas effect, can not form the environment that is suitable for animal cell culture most.For example use the ventilation of deep layer bubbling easily responsive zooblast to be damaged; The ventilation of use cage, though avoided bubble that zooblast is damaged, oxygen mass transfer coefficient is little, can not satisfy the oxygen consumption requirement when cultivating high-density cells.
The utility model content
The utility model embodiment provides a kind of animal cell culture bioreactor, adopt single ventilating mode in order to solve existing animal cell culture bioreactor, can't when guaranteeing good mass transfer effect, keep low-shearing power, reduce problem the zooblast damage.
The utility model embodiment provides a kind of animal cell culture bioreactor, comprises
Tank body;
Intake ducting is communicated with source of the gas;
Also comprise:
Magnetic valve is arranged on the described intake ducting;
The ventilation assembly comprises the top layer breather line and the deep ventilation pipeline that is used for the gas of described intake ducting is fed described tank body deep layer that are used for the gas of described intake ducting is fed described tank body top layer;
Be used for going out pneumatic module with what the gas of described tank body was discharged;
Be used to detect the environmental parameter of described tank body, obtain the detection components of environmental parameter detected value;
Be used for difference, control the on-off control assembly that described magnetic valve opens or closes, be connected with detection components with described magnetic valve according to described environmental parameter detected value and preset value.
Described top layer breather line, an end is communicated with described intake ducting, and the other end is communicated with the top of described tank body.
Described deep ventilation pipeline, an end is communicated with described intake ducting, and the other end is communicated with described tank body and stretches into the bottom of described tank body;
Described animal cell culture bioreactor also comprises:
Gaseous diffuser is arranged at the end that described deep ventilation pipeline stretches into an end of described tank body bottom.
Described animal cell culture bioreactor also comprises:
First gas filter is arranged on the breather line of described top layer;
Second gas filter is arranged on the described deep ventilation pipeline.
Describedly go out pneumatic module and comprise:
The 3rd gas filter is arranged on the cover of described tank body.
Describedly go out pneumatic module and also comprise:
Outlet pipe is communicated with the delivery port of described the 3rd gas filter.
Described animal cell culture bioreactor also comprises:
The gas mixer, an end is communicated with described intake ducting, and the other end is communicated with described top layer breather line and deep ventilation pipeline.
Described animal cell culture bioreactor also comprises:
Ratio adjusting valve is arranged on the described intake ducting;
Be used for difference, control the angle control unit of the opening angle of described ratio adjusting valve, be connected with detection components with described ratio adjusting valve according to described environmental parameter detected value and preset value.
Described intake ducting comprises:
The a plurality of subtubes that connect different sources of the gas respectively.
Described detection components comprises:
Be used to detect the potential of hydrogen of described tank body, obtain first detecting unit of potential of hydrogen detected value;
Be used to detect the oxygen dissolving value of described tank body, obtain second detecting unit of oxygen dissolving value detected value;
Be used to detect the pressure of described tank body, obtain the 3rd detecting unit of detected pressure value.
Described animal cell culture bioreactor also comprises:
Manually-operated gate is arranged on the described intake ducting.
In the animal cell culture bioreactor that the utility model embodiment provides, the top layer breather line is mainly in order to ventilate with the pressure of control tank body to the intravital top layer of jar when carrying out bioreactor operation, the deep ventilation pipeline is mainly in order to the deep ventilation of tank body inner cell nutrient solution potential of hydrogen and the oxygen dissolving value with controlling tank cells in vivo nutrient solution, it can be passed to gas in the animal cell culture liquid well, guaranteed good mass transfer effect, improved the dissolved oxygen transmission coefficient, realized good bio-reactor environment control, keep low-shearing power simultaneously, reduced bubble and broken the damage of zooblast.
Description of drawings
Fig. 1 is the structural representation of a kind of animal cell culture bioreactor among the utility model embodiment;
Fig. 2 is the structural representation of second kind of animal cell culture bioreactor of the utility model embodiment;
Fig. 3 is the structural representation of the third animal cell culture bioreactor of the utility model embodiment;
Fig. 4 is the structural representation of the 4th kind of animal cell culture bioreactor of the utility model embodiment;
Fig. 5 is the structural representation of the 5th kind of animal cell culture bioreactor of the utility model embodiment.
Embodiment
In the animal cell culture bioreactor that the utility model embodiment provides, the top layer breather line is mainly in order to ventilate with the pressure of control tank body to the intravital top layer of jar when carrying out bioreactor operation, the deep ventilation pipeline is mainly in order to the deep ventilation of tank body inner cell nutrient solution potential of hydrogen and the oxygen dissolving value with controlling tank cells in vivo nutrient solution, it can be passed to gas in the animal cell culture liquid well, guaranteed good mass transfer effect, improved the dissolved oxygen transmission effect, realized good bio-reactor environment control, keep low-shearing power simultaneously, reduced bubble and broken the damage of zooblast.
In addition, detection components is obtained the environmental parameter detected value of tank body, the on-off control assembly is according to the difference of environmental parameter detected value and preset value, magnetic valve opens or closes on the automatic control intake ducting, make the environmental parameter of tank body maintain preset value, not only improve the control accuracy of tank body environmental parameter, and do not needed operator's real-time monitoring and frequent operation, reduced the manpower consumption.
As Fig. 1, a kind of animal cell culture bioreactor that the utility model embodiment provides comprises:
Tank body;
Intake ducting is communicated with source of the gas;
Also comprise:
Magnetic valve is arranged on the intake ducting;
The ventilation assembly comprises the top layer breather line and the deep ventilation pipeline that is used for the gas of intake ducting is fed the tank body deep layer that are used for the gas of intake ducting is fed the tank body top layer;
Be used for going out pneumatic module with what the gas of tank body was discharged;
Be used to detect the environmental parameter of tank body, obtain the detection components of environmental parameter detected value;
Be used for the difference according to environmental parameter detected value and preset value, the on-off control assembly that the control magnetic valve opens or closes is connected with detection components with magnetic valve.
Wherein, the environmental parameter of above-mentioned tank body comprises potential of hydrogen, oxygen dissolving value and the pressure etc. of tank body.The preset value of environmental parameter can be a discrete value, also can be a span.The gas that is input in the tank body is multiple, such as oxygen, carbonic acid gas, pressurized air and nitrogen etc.Wherein, input pressurized air, oxygen or nitrogen can be controlled the oxygen dissolving value of tank body, and input pressurized air can be controlled the pressure of tank body, and the input carbonic acid gas can be controlled the potential of hydrogen of tank body.
Describe each parts below respectively in detail:
1), ventilation assembly
The ventilation assembly comprises the top layer breather line and the deep ventilation pipeline that is used for the gas of intake ducting is fed the tank body deep layer that is used for the gas of intake ducting is fed the tank body top layer.
(1.1), exemplarily, as Fig. 2, an end of top layer breather line is communicated with intake ducting, and the other end is communicated with the top of tank body.
Wherein, tank body top refers to the middle above position of tank body, as top and other position that holds gas.
The top layer breather line is mainly in order to ventilate with the pressure of control tank body to the intravital top layer of jar when carrying out bioreactor operation.In the time will controlling the pressure of tank body, can use the top layer breather line to the ventilation of the top layer of tank body.
(1.2), exemplarily, as Fig. 2, an end of deep ventilation pipeline is communicated with intake ducting, and the other end is communicated with tank body and stretches into the tank body bottom.
Wherein, the tank body bottom refers to the middle following position of tank body, as bottom and other position that holds animal cell culture liquid.
The deep ventilation pipeline is mainly in order to the deep ventilation of tank body inner cell nutrient solution potential of hydrogen and the oxygen dissolving value with controlling tank cells in vivo nutrient solution, it can be passed to gas in the animal cell culture liquid well, guaranteed good mass transfer effect, improved the dissolved oxygen transmission coefficient, realized good bio-reactor environment control, keep low-shearing power simultaneously, reduced bubble and broken the damage of zooblast.
Preferably, Fig. 2 for another example, animal cell culture bioreactor also can comprise:
Gaseous diffuser is arranged at the end that the deep ventilation pipeline stretches into an end of tank body bottom.
The size of the aperture of gaseous diffuser and give vent to anger speed decision gas transfer speed and pair cell damage.Gaseous diffuser can be made by multiple materials such as stainless steel, glass, potteries, also can be made into broad varietys such as single hole, porous, sphere, annular, arm shape.
By the setting of gaseous diffuser, realized good gas transfer effect on the one hand, improved the dissolved oxygen transmission effect, realized better bio-reactor environment control; Reduce bubble simultaneously and produce shearing force, reduced the damage that zooblast is caused.
Gaseous diffuser below is the bio-reactor bottom, also agitator can be installed further, and gas evenly spreads in the tank body in the liquid by liquid agitation waveform in the tank body, realizes optimal gas mass transfer mixing effect.
(1.3), in order to guarantee the purity of gas in the tank body, above-mentioned animal cell culture bioreactor also can comprise:
First gas filter is arranged on the breather line of top layer;
Second gas filter is arranged on the deep ventilation pipeline.
By the setting of above-mentioned gas strainer, can carry out filtration sterilization to the gas that enters into tank body, guarantee to enter into the purity of the gas of tank body, to keep the demand that atmosphere surrounding in the tank body satisfies animal cell culture.
In addition, above-mentioned animal cell culture bioreactor also can comprise:
The gas mixer, an end is communicated with intake ducting, and the other end is communicated with top layer breather line and deep ventilation pipeline.
By the setting of above-mentioned gas mixer, can improve gas blended effect.
2), intake ducting
Input terminus is communicated with source of the gas, and output terminal is communicated with the ventilation assembly, is used to transmit gas.
Have multiplely owing to be input to gas in the tank body, therefore, intake ducting can comprise:
The a plurality of subtubes that connect different sources of the gas respectively, wherein, the delivery port of each subtube can be communicated with the input aperture of top layer breather line or the input aperture of deep ventilation pipeline, and each subtube is provided with one first magnetic valve.Each subtube is independent separately, independent respectively input gas.
Such as, a plurality of subtubes can be first subtubes that connects source of oxygen, connect second subtube of carbon dioxide source, connect the 3rd subtube of compressed air source, connect the 4th subtube of source nitrogen.
3), go out pneumatic module
Going out pneumatic module is used for the gas of tank body is discharged.
Exemplarily, as Fig. 3, the pneumatic module that goes out among Fig. 1 (or Fig. 2) comprises:
The 3rd gas filter is arranged on the cover of tank body.
Preferably, be arranged at first gas filter on the breather line of top layer, can directly replace with the 3rd gas filter, when promptly using the top layer breather line, can directly the top layer breather line be connected with the 3rd gas filter, make the 3rd gas filter play the effect of gas in the breather line of filtration, purification top layer, can simplify the structure of bio-reactor like this.
In addition, Fig. 3 for another example, going out pneumatic module can also comprise:
Outlet pipe is communicated with the delivery port of the 3rd gas filter.
Wherein, also can be provided with magnetic valve on the outlet pipe, at this moment, the on-off control assembly also can be according to the difference of environmental parameter detected value and preset value, opening or closing of magnetic valve on the control outlet pipe, like this, the on-off control assembly just can be controlled entering and discharging of the interior gas of tank body.
4), detection components
The detection components online in real time detects the environmental parameter of tank body.
Because the environmental parameter of tank body comprises potential of hydrogen, oxygen dissolving value and the pressure etc. of tank body, at this moment, as Fig. 4, the detection components among Fig. 1 (or Fig. 2,3) can further comprise:
Be used to detect the potential of hydrogen of tank body, obtain first detecting unit of potential of hydrogen detected value;
Be used to detect the oxygen dissolving value of tank body, obtain second detecting unit of oxygen dissolving value detected value;
Be used to detect the pressure of tank body, obtain the 3rd detecting unit of detected pressure value.
Wherein, each detecting unit all is connected with the on-off control assembly.
5), on-off control assembly
The on-off control assembly can realize that certainly, intermediate relay can not need yet by A/D modular converter, central processing unit and intermediate relay, and sends signal by direct rly. to magnetic valve, controls its unlatching or close to get final product.
For speed and the precision that improves control, as Fig. 5, the animal cell culture bioreactor among Fig. 1 (or Fig. 2,3,4) also can comprise:
Ratio adjusting valve is arranged on the intake ducting;
Be used for the difference according to environmental parameter detected value and preset value, the angle control unit of the opening angle of control ratio variable valve is connected with detection components with ratio adjusting valve.
In addition, also ratio adjusting valve can be set on the outlet pipe, at this moment, the angle control unit also can be according to the difference of environmental parameter detected value and preset value, the opening angle of the ratio adjusting valve on the control outlet pipe.
Wherein, the opening angle of ratio adjusting valve can be controlled, and such as when the difference of environmental parameter detected value and preset value is big, the angle control unit can be transferred the opening angle of ratio adjusting valve big, make gas flow rates bigger, be beneficial to the environmental parameter rapid adjustment to preset value; And when the difference of environmental parameter detected value and preset value when less or difference diminishes gradually, the angle control unit can be turned the opening angle of ratio adjusting valve down, makes gas flow rates less, is beneficial to environmental parameter is accurately adjusted to preset value.
In addition, above-mentioned on-off control assembly and angle control unit are described respectively, but should be understood that, the two also can be to be realized by a control unit.
In the animal cell culture bioreactor that the utility model embodiment provides, be provided with detection components and control unit (comprising on-off control assembly and angle control unit), wherein, detection components is obtained the environmental parameter detected value of tank body, control unit is according to the difference of environmental parameter detected value and preset value, automatically the valve (comprising magnetic valve and ratio adjusting valve) on control intake ducting or the outlet pipe opens or closes, make the environmental parameter of tank body maintain preset value, not only improved the control accuracy of tank body environmental parameter, and do not need operator's real-time monitoring and frequent operation, reduced the manpower consumption.
Certainly, in the animal cell culture bioreactor that the utility model embodiment provides, also can comprise:
Manually-operated gate is arranged on the intake ducting.
In addition, outlet pipe also can be provided with manually-operated gate.
By the setting of above-mentioned manually-operated gate, automatic control mode and manual master mode are combined, operator can select to adopt automatic control mode or manual master mode as required.Certainly, when automatic control mode, should guarantee that manually-operated gate is in opened condition, otherwise automatic control section also can't be realized the automatic adjusting of tank body environmental parameter.
The above is a preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from principle described in the utility model; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (11)
1. an animal cell culture bioreactor comprises
Tank body;
Intake ducting is communicated with source of the gas;
It is characterized in that, also comprise:
Magnetic valve is arranged on the described intake ducting;
The ventilation assembly comprises the top layer breather line and the deep ventilation pipeline that is used for the gas of described intake ducting is fed described tank body deep layer that are used for the gas of described intake ducting is fed described tank body top layer;
Be used for going out pneumatic module with what the gas of described tank body was discharged;
Be used to detect the environmental parameter of described tank body, obtain the detection components of environmental parameter detected value;
Be used for difference, control the on-off control assembly that described magnetic valve opens or closes, be connected with detection components with described magnetic valve according to described environmental parameter detected value and preset value.
2. animal cell culture bioreactor as claimed in claim 1 is characterized in that,
Described top layer breather line, an end is communicated with described intake ducting, and the other end is communicated with the top of described tank body.
3. animal cell culture bioreactor as claimed in claim 1 is characterized in that,
Described deep ventilation pipeline, an end is communicated with described intake ducting, and the other end is communicated with described tank body and stretches into the bottom of described tank body;
Described animal cell culture bioreactor also comprises:
Gaseous diffuser is arranged at the end that described deep ventilation pipeline stretches into an end of described tank body bottom.
4. animal cell culture bioreactor as claimed in claim 1 is characterized in that, also comprises:
First gas filter is arranged on the breather line of described top layer;
Second gas filter is arranged on the described deep ventilation pipeline.
5. animal cell culture bioreactor as claimed in claim 1 is characterized in that,
Describedly go out pneumatic module and comprise:
The 3rd gas filter is arranged on the cover of described tank body.
6. animal cell culture bioreactor as claimed in claim 5 is characterized in that,
Describedly go out pneumatic module and also comprise:
Outlet pipe is communicated with the delivery port of described the 3rd gas filter.
7. as arbitrary described animal cell culture bioreactor among the claim 1-6, it is characterized in that, also comprise:
The gas mixer, an end is communicated with described intake ducting, and the other end is communicated with described top layer breather line and deep ventilation pipeline.
8. animal cell culture bioreactor as claimed in claim 1 is characterized in that, also comprises:
Ratio adjusting valve is arranged on the described intake ducting;
Be used for difference, control the angle control unit of the opening angle of described ratio adjusting valve, be connected with detection components with described ratio adjusting valve according to described environmental parameter detected value and preset value.
9. animal cell culture bioreactor as claimed in claim 1 is characterized in that,
Described intake ducting comprises:
The a plurality of subtubes that connect different sources of the gas respectively.
10. as claim 1 or 9 described animal cell culture bioreactors, it is characterized in that,
Described detection components comprises:
Be used to detect the potential of hydrogen of described tank body, obtain first detecting unit of potential of hydrogen detected value;
Be used to detect the oxygen dissolving value of described tank body, obtain second detecting unit of oxygen dissolving value detected value;
Be used to detect the pressure of described tank body, obtain the 3rd detecting unit of detected pressure value.
11. animal cell culture bioreactor as claimed in claim 1 is characterized in that, also comprises:
Manually-operated gate is arranged on the described intake ducting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206686657U CN201915101U (en) | 2010-12-20 | 2010-12-20 | Bioreactor for animal cell culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206686657U CN201915101U (en) | 2010-12-20 | 2010-12-20 | Bioreactor for animal cell culture |
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| CN201915101U true CN201915101U (en) | 2011-08-03 |
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| CN2010206686657U Expired - Fee Related CN201915101U (en) | 2010-12-20 | 2010-12-20 | Bioreactor for animal cell culture |
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| CN (1) | CN201915101U (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667043A (en) * | 2013-04-01 | 2014-03-26 | 中国航天员科研训练中心 | Environment-controlled experimental device for researching shear stress cytological effects of fluid |
| CN106754326A (en) * | 2017-01-13 | 2017-05-31 | 武汉互创联合科技有限公司 | A kind of devices and methods therefor for controlling single bin to supply break-make |
| CN107532132A (en) * | 2015-02-09 | 2018-01-02 | 优尼沃赛尔斯公司 | For producing the systems, devices and methods of cell and/or cell products |
| CN109258441A (en) * | 2018-10-24 | 2019-01-25 | 杭州师范大学 | Plant forms observe hydroponic device and application method |
| CN109373853A (en) * | 2018-10-24 | 2019-02-22 | 杭州师范大学 | Water culture and root measurement device and application method |
| WO2019206207A1 (en) * | 2018-04-26 | 2019-10-31 | 上海久博生物工程有限公司 | Active ventilation assembly, active-ventilation-type bioreactor and cell culture device |
| CN113999767A (en) * | 2021-11-02 | 2022-02-01 | 安及义实业（上海）有限公司 | Stainless steel bioreactor for mammalian cell culture and use method thereof |
| CN114561291A (en) * | 2022-03-21 | 2022-05-31 | 广州齐志生物工程设备有限公司 | 500ml small-sized one-control multi-cell bioreactor |
-
2010
- 2010-12-20 CN CN2010206686657U patent/CN201915101U/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667043A (en) * | 2013-04-01 | 2014-03-26 | 中国航天员科研训练中心 | Environment-controlled experimental device for researching shear stress cytological effects of fluid |
| CN103667043B (en) * | 2013-04-01 | 2015-07-01 | 中国航天员科研训练中心 | Environment-controlled experimental device for researching shear stress cytological effects of fluid |
| CN107532132A (en) * | 2015-02-09 | 2018-01-02 | 优尼沃赛尔斯公司 | For producing the systems, devices and methods of cell and/or cell products |
| CN106754326A (en) * | 2017-01-13 | 2017-05-31 | 武汉互创联合科技有限公司 | A kind of devices and methods therefor for controlling single bin to supply break-make |
| CN106754326B (en) * | 2017-01-13 | 2023-10-20 | 武汉互创联合科技有限公司 | Device and method for controlling on-off of air supply of single bin |
| WO2019206207A1 (en) * | 2018-04-26 | 2019-10-31 | 上海久博生物工程有限公司 | Active ventilation assembly, active-ventilation-type bioreactor and cell culture device |
| CN109258441A (en) * | 2018-10-24 | 2019-01-25 | 杭州师范大学 | Plant forms observe hydroponic device and application method |
| CN109373853A (en) * | 2018-10-24 | 2019-02-22 | 杭州师范大学 | Water culture and root measurement device and application method |
| CN109258441B (en) * | 2018-10-24 | 2023-12-08 | 杭州师范大学 | Plant morphology observation hydroponic device and use method |
| CN109373853B (en) * | 2018-10-24 | 2024-03-01 | 杭州师范大学 | Plant hydroponic and root length measuring device and using method |
| CN113999767A (en) * | 2021-11-02 | 2022-02-01 | 安及义实业（上海）有限公司 | Stainless steel bioreactor for mammalian cell culture and use method thereof |
| CN114561291A (en) * | 2022-03-21 | 2022-05-31 | 广州齐志生物工程设备有限公司 | 500ml small-sized one-control multi-cell bioreactor |
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Granted publication date: 20110803 Termination date: 20161220 |