CN106754326B - Device and method for controlling on-off of air supply of single bin - Google Patents
Device and method for controlling on-off of air supply of single bin Download PDFInfo
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- CN106754326B CN106754326B CN201710024492.1A CN201710024492A CN106754326B CN 106754326 B CN106754326 B CN 106754326B CN 201710024492 A CN201710024492 A CN 201710024492A CN 106754326 B CN106754326 B CN 106754326B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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Abstract
The invention relates to a device for controlling the on-off of air supply of a single bin, which comprises a bin, a first electromagnetic valve, a second electromagnetic valve, a detection switch, a monitoring system circuit board and an air path circulation system, wherein the first electromagnetic valve, the second electromagnetic valve and the detection switch are arranged on the bin, the monitoring system circuit board and the air path circulation system are connected with the first electromagnetic valve, the second electromagnetic valve and the detection switch, the bin comprises a cavity and a bin cover which is covered on the cavity, the first electromagnetic valve and the second electromagnetic valve are respectively arranged on the side wall of the bin and are connected with the air path circulation system, the first electromagnetic valve is arranged at an air inlet of the bin, and the second electromagnetic valve is arranged at an air outlet of the bin. The invention reduces or even avoids the waste caused by the leakage of the required mixed gas; the method reduces and avoids the mixing of external gas into the gas circuit circulation system, and causes the change of the concentration of oxygen and carbon dioxide gas in the gas circuit circulation system, thereby avoiding the time for the gas circuit circulation system to readjust the concentration ratio of the required gas to the normal value.
Description
Technical Field
The invention relates to a device and a method for controlling on-off of air supply of a single bin.
Background
In various devices and apparatuses for human embryonic cell culture, a gaseous environment of specific concentrations of oxygen and carbon dioxide is required. For this purpose, three gas tanks of carbon dioxide, oxygen and nitrogen are used in the market, which are premixed but have high price, to provide a gas environment for the three gas tanks; there is also a method of producing a gaseous environment required for embryo cell culture by mixing carbon dioxide gas and nitrogen gas in a predetermined concentration ratio in real time. The mixed gas flows in the gas path circulation system for recycling. However, when the cover is opened, the concentration of the mixed gas may be unnecessarily changed, such as leakage of the culture gas, channeling of outside air and other gases into the gas circuit circulation system of the incubator, and the like.
The existing countermeasures for the change of the gas in the bin when the bin cover is opened are not interfered or an acquiescent mode is adopted, so that the waste of the required mixed gas is caused, the instability of a gas path circulating system is also caused, and even the gas path is polluted, so that the filter is required to be replaced frequently, and a series of unnecessary waste is caused.
Disclosure of Invention
The invention aims at providing a device for controlling the on-off of air supply of a single bin and a method thereof, which reduce and avoid the pollution of an air path circulation system.
The invention adopts the technical scheme that: the utility model provides a device of control single bin air feed break-make, its characterized in that, is in including the bin, setting first solenoid valve, second solenoid valve and the detection switch on the bin, with monitored control system circuit board and the gas circuit circulation system that first solenoid valve, second solenoid valve and detection switch are connected, the bin includes cavity and lid locate the storehouse lid on the cavity, first solenoid valve and second solenoid valve set up respectively the lateral wall of bin, and will bin with gas circuit circulation system connects, first solenoid valve sets up the air inlet department of bin, the second solenoid valve sets up the gas outlet department of bin, detection switch is used for detecting the state of the storehouse lid of bin, monitored control system circuit board is used for monitoring the state of detection switch, simultaneously according to the state control of detection switch the power supply condition of first solenoid valve and second solenoid valve, thereby further control the break-make of first solenoid valve and second solenoid valve.
The technical scheme has the effects that: the waste caused by the leakage of the required mixed gas is reduced or even avoided; the method reduces and avoids the mixing of external gas into the gas circuit circulation system, and causes the change of the concentration of oxygen and carbon dioxide gas in the gas circuit circulation system, thereby avoiding the time for the gas circuit circulation system to readjust the concentration proportion of the required gas to a normal value; the unpredictable damage caused by the mixing of uncertain external gas into the gas path circulation system is reduced and avoided.
Further, the first electromagnetic valve and the second electromagnetic valve are integrated with an electromagnetic coil and a metal reed, and have two working states: on and off.
Further, the detection switch is arranged on the side wall of the bin and is close to the second electromagnetic valve, and the detection switch has two working states: pressing and bouncing, wherein the pressing indicates that a bin cover of the bin is closed, and the detection switch is pressed by the pressure of the bin cover; when the bin cover is sprung, the bin cover of the bin is opened, and the detection switch is sprung under the action of the spring force in the detection switch, so that the initial sprung state is restored.
Further, when the detection switch is sprung, the monitoring system circuit can control the first electromagnetic valve and the second electromagnetic valve to enter a cut-off state, and the bin of the bin cover is ensured to be isolated from the gas circuit circulation system.
Further, when the detection switch is pressed down, the monitoring system circuit board controls the first electromagnetic valve to be conducted first until the cavity of the bin is filled with mixed gas in the gas circuit circulation system, and then controls the second electromagnetic valve to be conducted, so that the bin returns to the gas circuit circulation system.
The invention adopts the technical scheme that: a method of controlling the on-off of a single bin supply, comprising the steps of: s1: the device comprises a bin, a first electromagnetic valve, a second electromagnetic valve and a detection switch, wherein the first electromagnetic valve, the second electromagnetic valve and the detection switch are arranged on the bin, a monitoring system circuit board and an air path circulating system are connected with the first electromagnetic valve, the second electromagnetic valve and the detection switch, the bin comprises a cavity and a bin cover which is covered on the cavity, the first electromagnetic valve and the second electromagnetic valve are respectively arranged on the side wall of the bin, the first electromagnetic valve and the second electromagnetic valve are used for connecting the bin and the air path circulating system, the first electromagnetic valve is arranged at an air inlet of the bin, the second electromagnetic valve is arranged at an air outlet of the bin, the detection switch is used for detecting the closing state of the bin cover of the bin, and the monitoring system circuit board is used for monitoring the state change of the detection switch, and simultaneously controlling the power supply conditions of the first electromagnetic valve and the second electromagnetic valve according to the state of the detection switch, so that the on-off of the first electromagnetic valve and the second electromagnetic valve is further controlled; s2: step S3 is executed when the bin cover of the bin is opened, and step S4 is executed when the bin cover of the bin is closed; s3: when the detection switch is sprung, the monitoring system circuit board controls the first electromagnetic valve and the second electromagnetic valve to be cut off simultaneously, and the bin is isolated from the gas circuit circulation system; s4: when the detection switch is pressed down, the monitoring system circuit board controls the first electromagnetic valve to be conducted first until the cavity of the bin is filled with mixed gas in the gas circuit circulation system, and then controls the second electromagnetic valve to be conducted, so that the bin returns to the gas circuit circulation system.
The technical scheme has the effects that: the waste caused by the leakage of the required mixed gas is reduced or even avoided; the method reduces and avoids the mixing of external gas into the gas circuit circulation system, and causes the change of the concentration of oxygen and carbon dioxide gas in the gas circuit circulation system, thereby avoiding the time for the gas circuit circulation system to readjust the concentration proportion of the required gas to a normal value; the unpredictable damage caused by the mixing of uncertain external gas into the gas path circulation system is reduced and avoided.
Drawings
Fig. 1 is a schematic structural diagram of a device for controlling on-off of air supply of a single bin according to the present invention.
Fig. 2 is a schematic view of the structure of the bin cover of fig. 1 after being opened.
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
Referring to fig. 1 and fig. 2, the device for controlling the on-off of air supply of a single bin provided by the invention comprises a bin 1, a first electromagnetic valve 2, a second electromagnetic valve 3 and a detection switch 4 which are arranged on the bin 1, a monitoring system circuit board 5 and an air path circulation system which are connected with the first electromagnetic valve 2, the second electromagnetic valve 3 and the detection switch 4.
The chamber 1 includes a cavity 11 and a cover 12 covering the cavity 11, and the cover 12 may be opened.
The first electromagnetic valve 2 and the second electromagnetic valve 3 are respectively arranged on two opposite side walls of the chamber 1, and connect the chamber 1 with the air path circulation system. The first electromagnetic valve 2 is arranged at the air inlet of the bin 1, and the second electromagnetic valve 3 is arranged at the air outlet of the bin 1. The first electromagnetic valve 2 and the second electromagnetic valve 3 are integrated with electromagnetic coils and metal reeds.
The first solenoid valve 2 and the second solenoid valve 3 have two working states, namely on and off. When the electromagnetic coil of the first electromagnetic valve 2 or the second electromagnetic valve 3 is supplied with power, the metal reed inside the first electromagnetic valve 2 or the second electromagnetic valve 3 is attracted. When the power is not supplied, the metal spring sheet in the first electromagnetic valve 2 or the second electromagnetic valve 3 is recovered to the initial natural state under the action of the self-elastic force.
When the first electromagnetic valve 2 or the second electromagnetic valve 3 is turned on, gas can flow through the first electromagnetic valve 2 or the second electromagnetic valve 3 to enter the bin 1, and when the first electromagnetic valve or the second electromagnetic valve 3 is turned off, gas cannot flow into the bin 1.
The detection switch 4 is provided on the side wall of the chamber 1, and is close to the second solenoid valve 3. The detection switch 4 has two operating states, namely pressing and bouncing. When pressed, the bin cover 12 of the bin 1 is closed, and the detection switch 4 is pressed by the pressure of the bin cover 12; when the bin cover 12 of the bin 1 is opened, the detection switch 4 is sprung under the action of the spring force in the detection switch, and the initial sprung state is restored.
The monitoring system circuit board 5 is used for detecting the pressing and bouncing states of the switch 4 and controlling whether and when to supply power to the electromagnetic coils of the first electromagnetic valve 2 and the second electromagnetic valve 3 according to logic requirements, so as to further determine the on-off state of the first electromagnetic valve 2 and the second electromagnetic valve 3.
The monitoring system circuit board 5 monitors the state change of the detection switch 4 at any time, and when the detection switch 4 bounces, the monitoring system circuit board 5 controls the first electromagnetic valve 2 and the second electromagnetic valve 3 to enter a cut-off state, so that the bin 1 of the opened bin cover 12 is ensured to be isolated from the gas circuit circulation system at the first time.
When the detection switch 4 is pressed, the monitoring system circuit board 5 controls the first electromagnetic valve 2 and the second electromagnetic valve 3 to enter a conducting state successively. The algorithm obtained by theoretical calculation and actual test optimization is obtained when the time difference is in sequence, so that the lowest influence and harm are brought to the gas path circulation system when the isolated bin 1 can return to the gas path circulation system again.
When the bin cover 12 of the bin 1 is manually lifted, the pressure exerted by the bin cover 12 on the self-contained spring in the detection switch 4 can disappear, and the detection switch 4 can be automatically switched from the pressed state to the sprung state under the action of the spring force of the self-contained spring. The change in state of the detection switch 4 is monitored by the circuit board 5 of the monitoring system. In contrast, when the cover 12 of the bin 1 is closed, the pressure of the cover 12 of the bin 1 received by the detection switch 4 is larger than the elastic force of the spring of the detection switch 4, the detection switch 4 is changed from the sprung state to the pressed state, and similarly, the change of the detection switch state caused by the process is also monitored by the circuit board of the monitoring system in fig. 5.
When the monitoring system circuit board 5 monitors that the state of the detection switch 4 is changed from the pressed state to the sprung state, the bin cover 12 of the bin 1 is judged to be opened, and meanwhile, the power supply states of the electromagnetic coils of the first electromagnetic valve 2 and the second electromagnetic valve 3 are changed, so that the two electromagnetic valves are in the cut-off state at the same time, and thus, the first electromagnetic valve 2 can prevent mixed gas in the gas path circulation system from entering the cavity 11 of the bin 1 and leaking into the atmosphere; after the second solenoid valve 3 is in the closed state, the gas in the cavity 11 exposed to the atmosphere is prevented from being continuously pumped into the gas path circulation system. The chamber 1 with the lid 12 opened will then be isolated from the gas circuit circulation system when the first and second solenoid valves 2, 3 are closed.
When the monitoring system circuit board 5 monitors that the state of the detection switch 4 is changed from the bouncing state to the pressing state, the bin cover 12 of the bin 1 is judged to be closed, and meanwhile, the power supply states of the electromagnetic coils of the first electromagnetic valve 2 and the second electromagnetic valve 3 are changed, so that the two electromagnetic valves are in a conducting state successively. The first electromagnetic valve 2 is turned on in advance, the mixed gas in the gas circuit circulation system flows into the cavity 11 of the chamber 1, and after a preset period of time, the cavity 11 of the chamber 1 is filled with the required mixed gas. At this time, the second electromagnetic valve 3 is conducted, and the bin 1 smoothly returns to the gas path circulation system. The chamber 1 which is completely isolated from the gas path circulation system can be re-integrated into the gas path circulation system under the condition of minimum mixed gas concentration proportion change and minimum mixed gas loss, and the embryo cell culture work can be restarted.
It will be appreciated that, regarding the time algorithm of refilling the independent chamber 1 with the effective mixed gas and returning to the gas circuit circulation system, the time can be specifically calculated according to the volume of the chamber 1 and the flow rate of the gas in the gas circuit circulation system, and the obtained time is set to the monitoring system circuit board 5, so that the problems of gas waste and time waste are more effectively solved.
Thus, the device for controlling the on-off of the air supply of the single bin provided by the invention can achieve the following beneficial effects:
1. the waste caused by the leakage of the required mixed gas is reduced or even avoided;
2. the method reduces and avoids the mixing of external gas into the gas circuit circulation system, and causes the change of the concentration of oxygen and carbon dioxide gas in the gas circuit circulation system, thereby avoiding the time for the gas circuit circulation system to readjust the concentration proportion of the required gas to a normal value;
3. the unpredictable damage caused by the mixing of uncertain external gas into the gas path circulation system is reduced and avoided.
The invention also relates to a method for controlling the on-off of the air supply of the single bin, which comprises the following steps:
s1: there is provided a device for controlling on-off of air supply of a single bin, the device comprising a bin 1, a first electromagnetic valve 2, a second electromagnetic valve 3 and a detection switch 4 which are arranged on the bin 1, a monitoring system circuit board 5 and an air path circulation system which are connected with the first electromagnetic valve 2, the second electromagnetic valve 3 and the detection switch 4, the bin 1 comprises a cavity 11 and a bin cover 12 which is covered on the cavity 11, the bin cover 12 can be lifted, the first electromagnetic valve 2 and the second electromagnetic valve 3 are respectively arranged on the side wall of the bin 1, the first electromagnetic valve 2 and the second electromagnetic valve 3 are respectively connected with the bin 1 and the air path circulation system, the first electromagnetic valve 2 is arranged at an air inlet of the bin 1, the second electromagnetic valve 3 is arranged at an air outlet of the bin 1, the detection switch 4 is used for detecting the closed state of the bin cover 12 of the bin 1, the monitoring system circuit board 5 is used for monitoring the state change of the detection switch 4, and simultaneously controlling the power supply condition of the first electromagnetic valve 2 and the second electromagnetic valve 3 according to the state of the detection switch 4, thereby further controlling on-off of the first electromagnetic valve 2 and the second electromagnetic valve 3;
s2: step S3 is performed when the cover 12 of the chamber 1 is opened, and step S4 is performed when the cover 12 of the chamber 1 is closed;
s3: the detection switch 4 is sprung, the monitoring system circuit board 5 controls the first electromagnetic valve 2 and the second electromagnetic valve 3 to be cut off simultaneously, and the bin 1 is isolated from the gas circuit circulation system;
s4: the detection switch 4 is pressed down, the monitoring system circuit board 5 controls the first electromagnetic valve 2 to be conducted firstly until the cavity 11 of the bin 1 is filled with mixed gas in the gas circuit circulation system, and the monitoring system circuit board 5 controls the second electromagnetic valve 3 to be conducted again, so that the bin 1 returns to the gas circuit circulation system.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (3)
1. The device for controlling the on-off of the air supply of the single bin is characterized by comprising a bin, a first electromagnetic valve, a second electromagnetic valve and a detection switch which are arranged on the bin, a monitoring system circuit board and an air path circulating system which are connected with the first electromagnetic valve, the second electromagnetic valve and the detection switch, wherein the bin comprises a cavity and a bin cover which is covered on the cavity, the first electromagnetic valve and the second electromagnetic valve are respectively arranged on the side wall of the bin and are connected with the air path circulating system, the first electromagnetic valve is arranged at an air inlet of the bin, the second electromagnetic valve is arranged at an air outlet of the bin, the detection switch is used for detecting the state of the bin cover of the bin, and the monitoring system circuit board is used for monitoring the state of the detection switch and controlling the power supply condition of the first electromagnetic valve and the second electromagnetic valve according to the state of the detection switch, so that the on-off of the first electromagnetic valve and the second electromagnetic valve is further controlled;
the detection switch is arranged on the side wall of the bin and is close to the second electromagnetic valve, and the detection switch has two working states: pressing and bouncing, wherein the pressing indicates that a bin cover of the bin is closed, and the detection switch is pressed by the pressure of the bin cover; when the bin cover of the bin is opened, the detection switch is sprung under the action of the spring force in the detection switch, and the initial spring state is restored;
when the detection switch is sprung, the monitoring system circuit controls the first electromagnetic valve and the second electromagnetic valve to enter a cut-off state, so that the bin of the bin cover is ensured to be isolated from the gas circuit circulation system;
when the detection switch is pressed down, the monitoring system circuit board controls the first electromagnetic valve to be conducted first until the cavity of the bin is filled with mixed gas in the gas circuit circulation system, and then controls the second electromagnetic valve to be conducted, so that the bin returns to the gas circuit circulation system.
2. The apparatus for controlling the on-off of a single bin supply according to claim 1, wherein: the first electromagnetic valve and the second electromagnetic valve are integrated with an electromagnetic coil and a metal reed and have two working states: on and off.
3. A method for controlling the on-off of air supply to a single bin, comprising the steps of:
s1: the device comprises a bin, a first electromagnetic valve, a second electromagnetic valve and a detection switch, wherein the first electromagnetic valve, the second electromagnetic valve and the detection switch are arranged on the bin, a monitoring system circuit board and an air path circulating system are connected with the first electromagnetic valve, the second electromagnetic valve and the detection switch, the bin comprises a cavity and a bin cover which is covered on the cavity, the first electromagnetic valve and the second electromagnetic valve are respectively arranged on the side wall of the bin, the first electromagnetic valve and the second electromagnetic valve are used for connecting the bin and the air path circulating system, the first electromagnetic valve is arranged at an air inlet of the bin, the second electromagnetic valve is arranged at an air outlet of the bin, the detection switch is used for detecting the closing state of the bin cover of the bin, and the monitoring system circuit board is used for monitoring the state change of the detection switch, and simultaneously controlling the power supply conditions of the first electromagnetic valve and the second electromagnetic valve according to the state of the detection switch, so that the on-off of the first electromagnetic valve and the second electromagnetic valve is further controlled; the detection switch is arranged on the side wall of the bin and is close to the second electromagnetic valve, and the detection switch has two working states: pressing and bouncing, wherein the pressing indicates that a bin cover of the bin is closed, and the detection switch is pressed by the pressure of the bin cover; when the bin cover of the bin is opened, the detection switch is sprung under the action of the spring force in the detection switch, and the initial spring state is restored;
s2: step S3 is executed when the bin cover of the bin is opened, and step S4 is executed when the bin cover of the bin is closed;
s3: when the detection switch is sprung, the monitoring system circuit board controls the first electromagnetic valve and the second electromagnetic valve to be cut off simultaneously, and the bin is isolated from the gas circuit circulation system;
s4: when the detection switch is pressed down, the monitoring system circuit board controls the first electromagnetic valve to be conducted first until the cavity of the bin is filled with mixed gas in the gas circuit circulation system, and then controls the second electromagnetic valve to be conducted, so that the bin returns to the gas circuit circulation system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710024492.1A CN106754326B (en) | 2017-01-13 | 2017-01-13 | Device and method for controlling on-off of air supply of single bin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710024492.1A CN106754326B (en) | 2017-01-13 | 2017-01-13 | Device and method for controlling on-off of air supply of single bin |
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| CN106754326A CN106754326A (en) | 2017-05-31 |
| CN106754326B true CN106754326B (en) | 2023-10-20 |
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| CN201825955U (en) * | 2009-07-10 | 2011-05-11 | 杭州安普生物工程有限公司 | Cell culture device |
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| JP4272850B2 (en) * | 2002-06-17 | 2009-06-03 | 学校法人慈恵大学 | Human cell / tissue culture system |
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2017
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| JP2005006519A (en) * | 2003-06-17 | 2005-01-13 | Espec Corp | Carbonic acid gas incubator |
| CN201386104Y (en) * | 2009-03-31 | 2010-01-20 | 北京天和瑞生物科技有限公司 | Bioreactor |
| CN201825955U (en) * | 2009-07-10 | 2011-05-11 | 杭州安普生物工程有限公司 | Cell culture device |
| CN201915101U (en) * | 2010-12-20 | 2011-08-03 | 北京清大天一科技有限公司 | Bioreactor for animal cell culture |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
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