CN205443326U - Cell culture device that reinforcing oxygen was supplied with - Google Patents
Cell culture device that reinforcing oxygen was supplied with Download PDFInfo
- Publication number
- CN205443326U CN205443326U CN201521136826.7U CN201521136826U CN205443326U CN 205443326 U CN205443326 U CN 205443326U CN 201521136826 U CN201521136826 U CN 201521136826U CN 205443326 U CN205443326 U CN 205443326U
- Authority
- CN
- China
- Prior art keywords
- oxygen
- cell culture
- oxygen concentration
- sensor
- gas cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a cell culture device that reinforcing oxygen was supplied with, includes cell culture case, baffle, dissolved oxygen consistency transmitter, carbon dioxide sensor, oxygen consistency transmitter, special cell culture bottle, oxygen concentration control system, carbon dioxide gas cylinder, nitrogen gas gas cylinder, sensor electrode jack, mixing pipe, cable, orifice plate, aerating device, tolerance consistency transmitter 0, oxygen gas cylinder and oxygen hose, be provided with special cell culture bottle on the baffle, special cell culture bottle's upper portion is provided with sensor electrode jack, be provided with dissolved oxygen consistency transmitter in the sensor electrode jack, cell culture incasement portion top is provided with carbon dioxide sensor and oxygen concentration sensor, dissolved oxygen consistency transmitter, carbon dioxide sensor, oxygen consistency transmitter are connected with oxygen concentration control system through the cable respectively, the utility model discloses simple structure, convenient to use, the oxygen that can effectively strengthen cell culture device is supplied with.
Description
Technical field
This utility model belongs to cell culture system, a kind of cell culture system strengthening oxygen supply.
Background technology
Cell culture invitro is to take out tissue alive or living cells in organism, it is inoculated in the liquid culture medium in specific culture vessel, wherein contain in a organized way or multiple nutritional components necessary to cell growth and breeding, and place it in simulation specific organism body in living environment incubator in, be allowed to grow, breed.
In scientific research, it is often necessary to learning gas environment especially anaerobic environment cell growth, divide, the impact of the process such as propagation.The conventional method that hypoxic cell is cultivated is to control in hypoxic cell incubator the concentration of oxygen in gaseous environment to be in anoxia level, then case inner cell culture fluid dissolved oxygen concentration also will be in anoxia level, thus realize simulating hypoxic cell cultivation.Cultivate owing to cell is immersed in liquid culture medium, monitoring in real time the constant key being to set up stable hypoxic cell cultivation of regulation culture liquid dissolved oxygen concentration.What the oxygen concentration sensor of traditional hypoxic cell incubator was monitored is the oxygen concentration of incubator internal gas environment, rather than culture fluid dissolved oxygen concentration.But, in cell cultivation process, owing to cell is bred in culture fluid in a large number, when the oxygen consumption rate of cell has exceeded the dissolved oxygen speed of culture fluid, then culture fluid dissolved oxygen concentration reduces, although now the oxygen concentration in incubator is constant, oxygen concentration when but culture fluid dissolved oxygen concentration is cultivated already below initial cell, this will cause the degree of cell hypoxia in cell cultivation process non-constant, affects the accuracy of experimental result the most at last, especially affects when extreme anoxia the most notable.
Additionally, the principal element affecting liquid dissolved oxygen concentration also includes salinity, atmospheric pressure etc..The composition of common basal cell culture fluid there are differences, especially salt content is different, the concentration causing culture fluid dissolved oxygen under identical oxygen concentration conditions is different, if now using different culture fluid to carry out cell cultivation, although setting identical incubator oxygen concentration, but culture fluid dissolved oxygen concentration is little same, then the degree of cell hypoxia is the most different.Usually need when carrying out cell experiment to be administered in culture fluid, especially heavy dose of administration artificially causes the change of culture fluid salt concentration, also the concentration that can cause culture fluid dissolved oxygen under identical oxygen concentration conditions is different, and now non-administered group is different from the degree of oxygen deficiency of administration group cell.And the change of the culture fluid acid-base value caused at different cultivation stage cell metabolism products, it is possible to cause the difference of culture fluid dissolved oxygen amount.Additionally, height above sea level difference then atmospheric pressure residing for laboratory is the most different, the most identical culture fluid dissolved oxygen concentration there is also difference, therefore, although the laboratory of Different Altitude sets identical incubator oxygen concentration, but owing to culture fluid dissolved oxygen concentration is different, the degree of cell hypoxia is the most different.The cell hypoxia degree caused by factors above is different, ultimately causes the inconsistent of experimental result.
Carry out hypoxic cell culture experiment and need constant culture fluid dissolved oxygen concentration condition, and traditional hypoxic cell incubator obviously can not be completely secured this condition, only cultivated the concordance of degree of oxygen deficiency by the dissolved oxygen concentration guarantee cell in monitoring regulation culture liquid.Therefore, it is necessary to research and develop a kind of cell culture system strengthening oxygen supply.
Utility model content
The purpose of this utility model is to provide a kind of cell culture system strengthening oxygen supply, solves the problem in background technology.
This utility model realizes by the following technical solutions:
nullA kind of cell culture system strengthening oxygen supply，Including cell culture incubator、Dividing plate、Dissolved oxygen concentration sensor、Carbon dioxide sensor、Oxygen concentration sensor、Special Tissue Culture Flask、Oxygen concentration control system、Dioxide bottle、Nitrogen gas cylinder、Sensor electrode jack、Mixture pipe、Cable、Orifice plate、Aerating device、Air volume regulating device、Oxygen gas cylinder and oxygen hose，It is provided with dividing plate in described cell culture incubator，Special Tissue Culture Flask it is provided with on described dividing plate，The top of described special Tissue Culture Flask is provided with sensor electrode jack，It is provided with dissolved oxygen concentration sensor in described sensor electrode jack，Described cell culture incubator inner upper is provided with carbon dioxide sensor and oxygen concentration sensor，Described dissolved oxygen concentration sensor、Carbon dioxide sensor、Oxygen concentration sensor is connected with oxygen concentration control system by cable respectively，Described oxygen concentration control system is connected with cell culture incubator by mixture pipe，Described oxygen concentration control system bottom has been respectively communicated with dioxide bottle by pipeline、Nitrogen gas cylinder、Oxygen gas cylinder，Described oxygen gas cylinder is connected by oxygen hose bottom special Tissue Culture Flask.
In this utility model, the pipeline between described oxygen gas cylinder and special Tissue Culture Flask is provided with air volume regulating device.
In this utility model, in described special Tissue Culture Flask, it is provided with orifice plate.
In this utility model, bottom described special Tissue Culture Flask, orifice plate side is provided with at least one aerating device.
In this utility model, described aerating device connects with oxygen hose.
In this utility model, the number of described dissolved oxygen concentration sensor is at least one.
Beneficial effect: this utility model simple in construction, easy to use, can effectively strengthen the oxygen supply of cell culture system.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model；
Fig. 2 is another structural representation of the present utility model.
Detailed description of the invention
For the technological means making this utility model realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with being specifically illustrating, this utility model is expanded on further.
nullSee Fig. 1，A kind of structural representation of the cell culture system strengthening oxygen supply，A kind of cell culture system strengthening oxygen supply，Including cell culture incubator 1、Dividing plate 2、Dissolved oxygen concentration sensor 3、Carbon dioxide sensor 4、Oxygen concentration sensor 5、Special Tissue Culture Flask 6、Oxygen concentration control system 7、Dioxide bottle 8、Nitrogen gas cylinder 9、Sensor electrode jack 10、Mixture pipe 11、Cable 12、Orifice plate 13、Aerating device 14、Air volume regulating device 15、Oxygen gas cylinder 16 and oxygen hose 17，It is provided with dividing plate 2 in described cell culture incubator 1，Special Tissue Culture Flask 6 it is provided with on described dividing plate 2，The top of described special Tissue Culture Flask 6 is provided with sensor electrode jack 10，It is provided with dissolved oxygen concentration sensor 3 in described sensor electrode jack 10，Described cell culture incubator 1 inner upper is provided with carbon dioxide sensor 4 and oxygen concentration sensor 5，Described dissolved oxygen concentration sensor 3、Carbon dioxide sensor 4、Oxygen concentration sensor 5 is connected with oxygen concentration control system 7 by cable 12 respectively，Described oxygen concentration control system 7 is connected with cell culture incubator 1 by mixture pipe 11，Described oxygen concentration control system 7 bottom has been respectively communicated with dioxide bottle 8 by pipeline、Nitrogen gas cylinder 9、Oxygen gas cylinder 16，Described oxygen gas cylinder 16 is connected by oxygen hose 17 with bottom special Tissue Culture Flask 6，It is provided with air volume regulating device 15 on pipeline between described oxygen gas cylinder 16 and special Tissue Culture Flask 6.
See Fig. 2, a kind of another structural representation of the cell culture system strengthening oxygen supply, being provided with orifice plate 13 in described special Tissue Culture Flask 6, bottom described special Tissue Culture Flask 6, orifice plate 13 side is provided with at least one aerating device 14, and described aerating device 14 connects with oxygen hose 17.The number of described dissolved oxygen concentration sensor 3 is at least one.
Embodiment 1
During use, the dissolved oxygen concentration in special Tissue Culture Flask 6 monitored in real time by dissolved oxygen concentration sensor 3, carbon dioxide sensor 4 and oxygen concentration sensor 5 monitor the gas concentration lwevel in cell culture incubator 1 and oxygen concentration in real time, and real-time Transmission is to oxygen concentration control system 7, oxygen concentration control system 7 is according to the dissolved oxygen concentration in special Tissue Culture Flask 6 and the gas concentration lwevel in cell culture incubator 1 and oxygen concentration, regulation mixture pipe 11 and the aeration quantity of oxygen hose 17, so that cell culture system is in optimal oxygen ranges.
Embodiment 2
Described aerating device 14 is positioned at the side of orifice plate 13, and beneficially dissolved oxygen concentration sensor 3 is under stable monitoring of environmental so that monitoring effect is more preferable.Described aerating device 14 is according to the size of special Tissue Culture Flask 6 and the requirement of culture environment, air volume regulating device 15 can control to adjust air pressure and the tolerance of inflation, dissolved oxygen concentration in the special Tissue Culture Flask 6 of convenient regulation in time so that in optimal culture environment.
Of the present utility model ultimate principle and principal character and of the present utility model advantage have more than been shown and described; skilled person will appreciate that of the industry; this utility model is not restricted to the described embodiments; described in above-described embodiment and description, principle of the present utility model is simply described; on the premise of without departing from this utility model spirit and scope; this utility model also has various changes and modifications; in the range of these changes and improvements both fall within claimed this utility model, this utility model claims scope and is defined by appending claims and equivalent thereof.
Claims (6)
- null1. the cell culture system strengthening oxygen supply，Including cell culture incubator、Dividing plate、Dissolved oxygen concentration sensor、Carbon dioxide sensor、Oxygen concentration sensor、Special Tissue Culture Flask、Oxygen concentration control system、Dioxide bottle、Nitrogen gas cylinder、Sensor electrode jack、Mixture pipe、Cable、Orifice plate、Aerating device、Air volume regulating device、Oxygen gas cylinder and oxygen hose，It is characterized in that，It is provided with dividing plate in described cell culture incubator，Special Tissue Culture Flask it is provided with on described dividing plate，The top of described special Tissue Culture Flask is provided with sensor electrode jack，It is provided with dissolved oxygen concentration sensor in described sensor electrode jack，Described cell culture incubator inner upper is provided with carbon dioxide sensor and oxygen concentration sensor，Described dissolved oxygen concentration sensor、Carbon dioxide sensor、Oxygen concentration sensor is connected with oxygen concentration control system by cable respectively，Described oxygen concentration control system is connected with cell culture incubator by mixture pipe，Described oxygen concentration control system bottom has been respectively communicated with dioxide bottle by pipeline、Nitrogen gas cylinder、Oxygen gas cylinder，Described oxygen gas cylinder is connected by oxygen hose bottom special Tissue Culture Flask.
- A kind of cell culture system strengthening oxygen supply the most according to claim 1, it is characterised in that be provided with air volume regulating device on the pipeline between described oxygen gas cylinder and special Tissue Culture Flask.
- A kind of cell culture system strengthening oxygen supply the most according to claim 1, it is characterised in that be provided with orifice plate in described special Tissue Culture Flask.
- A kind of cell culture system strengthening oxygen supply the most according to claim 1, it is characterised in that bottom described special Tissue Culture Flask, orifice plate side is provided with at least one aerating device.
- A kind of cell culture system strengthening oxygen supply the most according to claim 4, it is characterised in that described aerating device connects with oxygen hose.
- A kind of cell culture system strengthening oxygen supply the most according to claim 1, it is characterised in that the number of described dissolved oxygen concentration sensor is at least one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521136826.7U CN205443326U (en) | 2015-12-31 | 2015-12-31 | Cell culture device that reinforcing oxygen was supplied with |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521136826.7U CN205443326U (en) | 2015-12-31 | 2015-12-31 | Cell culture device that reinforcing oxygen was supplied with |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205443326U true CN205443326U (en) | 2016-08-10 |
Family
ID=56599579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521136826.7U Expired - Fee Related CN205443326U (en) | 2015-12-31 | 2015-12-31 | Cell culture device that reinforcing oxygen was supplied with |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205443326U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384714A (en) * | 2018-04-23 | 2018-08-10 | 苏州欧飞纳米科技有限公司 | A kind of gas-dynamic control system of biological cell reactor |
| CN110106082A (en) * | 2019-05-28 | 2019-08-09 | 中南大学湘雅医院 | Embedded cell hypoxia culture box |
-
2015
- 2015-12-31 CN CN201521136826.7U patent/CN205443326U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384714A (en) * | 2018-04-23 | 2018-08-10 | 苏州欧飞纳米科技有限公司 | A kind of gas-dynamic control system of biological cell reactor |
| CN110106082A (en) * | 2019-05-28 | 2019-08-09 | 中南大学湘雅医院 | Embedded cell hypoxia culture box |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201915101U (en) | Bioreactor for animal cell culture | |
| EP3167042A1 (en) | Bioreactor with interruptible gas supply | |
| Yang et al. | Improving microalgal growth with reduced diameters of aeration bubbles and enhanced mass transfer of solution in an oscillating flow field | |
| CN205443326U (en) | Cell culture device that reinforcing oxygen was supplied with | |
| CN104450519B (en) | The cell culture system of a kind of precise hard_drawn tuhes nutrient solution dissolved oxygen concentration | |
| CN204474690U (en) | The cell culture system of precise hard_drawn tuhes nutrient solution dissolved oxygen concentration | |
| CN201065407Y (en) | Open pressure program-controlled culture device for in vitro cell | |
| CN102643741B (en) | Trap type carbon replenishing device for culturing microalgae of opened pool and carbon replenishing method thereof | |
| CN202364681U (en) | Intelligent aeration system for fishpond | |
| CN203333366U (en) | Laboratory simulated seawater acidification system | |
| CN205547025U (en) | Culture apparatus capable of adjusting carbon dioxide concentration of water body | |
| CN104025904B (en) | The cultural method of Tricholoma matsutake (lto et lmai) Singer mycelia and special purpose device thereof | |
| CN104745472A (en) | Plant tissue culture tank | |
| CN205071898U (en) | A multipurpose plant for $monitoring aquatic organisms grows, activity action and population dynamics's water environment regulated and control | |
| CN205907269U (en) | Hair form root liquid culture plate | |
| CN204625652U (en) | For the device of ultra-clean chamber cell culture incubator transport of carbon dioxide | |
| CN207699598U (en) | A kind of cell culture rolling bottle that can be inflated in advance | |
| CN204939494U (en) | A kind of easy small-sized biological reactor | |
| CN205472689U (en) | Aseptic high concentration carbon dioxide generating device | |
| CN205576149U (en) | UNICOM's formula fermentation cylinder | |
| CN204759231U (en) | Plant is temperature control device for incubator | |
| CN207259510U (en) | A kind of controllable type air-breather for algae culture | |
| CN205607951U (en) | Little segasso sea ocean acidizing simulation experiment device | |
| CN204625650U (en) | A kind of disposable bioreactor | |
| CN202730135U (en) | Culturing device of culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 Termination date: 20171231 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |