CN207699598U - A kind of cell culture rolling bottle that can be inflated in advance - Google Patents
A kind of cell culture rolling bottle that can be inflated in advance Download PDFInfo
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- CN207699598U CN207699598U CN201721842552.2U CN201721842552U CN207699598U CN 207699598 U CN207699598 U CN 207699598U CN 201721842552 U CN201721842552 U CN 201721842552U CN 207699598 U CN207699598 U CN 207699598U
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Abstract
The utility model is related to technical field of cell culture, and disclose a kind of cell culture rolling bottle that can be inflated in advance, including pedestal, it is installed with supporting block at the top of the pedestal, the top movable of the supporting block is equipped with culture bottle, the top movable of the culture bottle is connected with sealing cover, the top of the pedestal, which is located on the left of culture bottle, is installed with support column, the bottom of the support column is installed with Rotary Machine, and the output shaft of the Rotary Machine is fixedly connected with one end through sealing cover and extends to the rotation axis inside culture bottle.This can inflate cell culture rolling bottle in advance, pass through being used cooperatively for gas bomb, gas tube, screw rod, stomata, gasket, air admission hole and gas vent, carbon dioxide gas can be poured in gas bomb in advance, adjust the pH value of culture medium in culture bottle, so that pH value is maintained in the appropriate range of cell Proliferation, accelerates the adherent process of cell and cell proliferation rate.
Description
Technical field
The utility model is related to technical field of cell culture, specially a kind of cell culture that can be inflated in advance, which is used, to be turned
Bottle.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology, no matter for
For entire biotechnology, or the biological cloning technology of one of them, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell, cell culture technology can by a cell by mass propgation at
For many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself
It is exactly the clone of cell, a large amount of cell or its metabolite is obtained by cell culture, rolling bottle is a kind of appearance of cell culture
Device.
Spinner culture is important method in cell culture and amplification, has sodium bicarbonate, carbon in common buffer system
Sour hydrogen sodium is easier to resolve into sodium carbonate and carbon dioxide gas, and Medium's PH Value is caused to rise, and conventional cell culture, which is used, to be turned
Bottle cannot adjust the pH value of culture medium in rolling bottle, pH value is excessively high usually since bottle stopper is closed by carbon dioxide gas
Cause cell adherent slow, cell proliferation rate is slow, also has part rolling bottle to use ventilated membrane, but the device culture medium of ventilated membrane holds
Yi Gan, and need to be put into carbon dioxide gas incubator, it has not been convenient to cell culture, so a kind of technical solution is proposed to solve
Problem certainly set forth above.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides one kind, and carbon dioxide gas can be poured in advance by having, and
When by carbon dioxide gas come adjust the pH value of culture medium in culture bottle to accelerate the adherent process of cell and cell Proliferation speed
The advantages that spending solves conventional cell culture with rolling bottle since bottle stopper is closed, cannot be adjusted by carbon dioxide gas
The pH value of culture medium in rolling bottle, pH value is excessively high to cause cell adherent slow, the slow problem of cell Proliferation.
(2) technical solution
Carbon dioxide gas can be poured in advance for realization is above-mentioned, trained in time by carbon dioxide gas to adjust in culture bottle
The pH value of base is supported to accelerate the purpose of cell adherent process and cell proliferation rate, the utility model provides following technical side
Case：A kind of cell culture rolling bottle that can be inflated in advance, including pedestal are installed with supporting block at the top of the pedestal,
The top movable of the supporting block is equipped with culture bottle, and the top movable of the culture bottle is connected with sealing cover, the pedestal
Top, which is located on the left of culture bottle, is installed with support column, and the bottom of the support column is installed with Rotary Machine, described turn
The output shaft of bottle machine is fixedly connected with one end through sealing cover and extends to the rotation axis inside culture bottle, the table of the rotation axis
Face is installed with blade, and gas bomb is installed on the right side of the inner wall of the culture bottle, and peace is fixed on the right side of the culture bottle
Culture bottle and gas bomb are sequentially passed through equipped with one end and extend to the gas tube inside gas bomb, the top grafting of the sealing cover
There is one end to sequentially pass through the screw rod of sealing cover and gas bomb, offer stomata at the top of the screw rod, the top of the screw rod with
Stomata corresponding position is movably installed with gasket, and air admission hole is offered on the left of the screw rod, and the left side of the screw rod is located at air inlet
Gas vent is offered below hole.
Preferably, feed pipe is fixedly connected at the top of the sealing cover, the inner wall of the feed pipe was fixedly connected with
Strainer.
Preferably, the quantity of the blade is no less than 14, and is uniformly distributed in the surface of rotation axis.
Preferably, it is located on the right side of the inner wall of the culture bottle below gas bomb and is installed with detection bottle, the detection
The inside of bottle is filled with bromothymol blue acid-base indicator.
Preferably, it is located on the right side of the inner wall of the culture bottle below detection bottle and is installed with graduated scale.
Preferably, the quantity of the gas vent is two, and the surface of the screw rod is coated between two gas vents
Orchil.
(3) advantageous effect
Compared with prior art, the utility model provides a kind of cell culture rolling bottle that can be inflated in advance, has
Following advantageous effect：
1, this can inflate cell culture rolling bottle in advance, by gas bomb, gas tube, screw rod, stomata, gasket, into
Being used cooperatively for stomata and gas vent, can in advance pour carbon dioxide gas in gas bomb, by gas bomb to culture bottle
Interior carbon dioxide gas adjusts the pH value of culture medium in culture bottle, and pH value is made to be maintained at the appropriate range of cell Proliferation
It is interior, accelerate the adherent process of cell and cell proliferation rate.
2, this can inflate cell culture rolling bottle in advance, by pedestal, supporting block, culture bottle, sealing cover, Rotary Machine,
Rotation axis and blade are used cooperatively, and culture solution in culture medium can be made to become dynamic from static state, mixing evenly, is conducive to cell
It is adherent and proliferation.
Description of the drawings
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model gas bomb structural schematic diagram.
In figure：1 pedestal, 2 supporting blocks, 3 culture bottles, 4 sealing covers, 5 Rotary Machines, 6 rotation axis, 7 blades, 8 gas bombs, 9 are filled
Tracheae, 10 screw rods, 11 stomatas, 12 gaskets, 13 air admission holes, 14 gas vents, 15 feed pipes, 16 filter screens, 17 detection bottles, 18 quarters
Spend ruler, 19 support columns.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
- 2 are please referred to Fig.1, the utility model provides a kind of technical solution：A kind of cell culture use turn that can be inflated in advance
Bottle, including pedestal 1, the top of pedestal 1 are installed with supporting block 2, and the top movable of supporting block 2 is equipped with culture bottle 3, culture
The top movable of bottle 3 is connected with sealing cover 4, and the left side that the top of pedestal 1 is located at culture bottle 3 is installed with support column 19, branch
The bottom of dagger 19 is installed with Rotary Machine 5, and the output shaft of Rotary Machine 5 is fixedly connected with one end through sealing cover 4 and extends
The surface of rotation axis 6 inside to culture bottle 3, rotation axis 6 is installed with blade 7, and the quantity of blade 7 is no less than 14,
And it is uniformly distributed in the surface of rotation axis 6, pass through pedestal 1, supporting block 2, culture bottle 3, sealing cover 4, Rotary Machine 5,6 and of rotation axis
Blade 7 is used cooperatively, and culture solution in culture medium can be made to become dynamic from static state, mixing evenly, is conducive to the adherent of cell
And proliferation, the inner wall right side of culture bottle 3 are installed with gas bomb 8, the right side of culture bottle 3 is installed with one end and sequentially passes through
Culture bottle 3 and gas bomb 8 simultaneously extend to the gas tube 9 inside gas bomb 8, and the top of sealing cover 4 is plugged with one end and sequentially passes through
The top of the screw rod 10 of sealing cover 4 and gas bomb 8, screw rod 10 offers stomata 11, top and 11 corresponding position of stomata of screw rod 10
It is movably installed with gasket 12, the left side of screw rod 10 offers air admission hole 13, and the left side of screw rod 10 is located at the lower section of air admission hole 13
Gas vent 14 is offered, the lower section that gas bomb 8 is located on the right side of the inner wall of culture bottle 3 is installed with detection bottle 17, detects bottle 17
Quantity of the inside filled with bromothymol blue acid-base indicator gas vent 14 be two, the surface of screw rod 10 is located at two rows
It is coated with orchil between stomata 14, passes through gas bomb 8, gas tube 9, screw rod 10, stomata 11, gasket 12, air admission hole 13
With being used cooperatively for gas vent 14, carbon dioxide gas can be poured in gas bomb 8 in advance, by gas bomb 8 to culture bottle 3
Interior carbon dioxide gas adjusts the pH value of culture medium in culture bottle 3, and pH value is made to be maintained at the appropriate range of cell Proliferation
It is interior, the adherent process of cell and cell proliferation rate are accelerated, bromothymol blue acid-base indicator is in density of carbon dioxide gas
When relatively low, solution colour is by blue greening, and when density of carbon dioxide gas is higher, solution is turned yellow again by blue greening, detects in bottle 17
Bromothymol blue acid-base indicator can monitor density of carbon dioxide gas in culture bottle 3, the surface of screw rod 10 is located at two
Orchil is coated between a gas vent 14 to be convenient for when controlling the position of gas vent 14 by the position of adjusting screw rod 10
It waits and differentiates the location of gas vent 14, the top of sealing cover 4 is fixedly connected with feed pipe 15, and the inner wall of feed pipe 15, which is fixed, to be connected
It is connected to filter screen 16, the lower section that detection bottle 17 is located on the right side of the inner wall of culture bottle 3 is installed with graduated scale 18, passes through feed pipe
15 injection culture mediums and cell mixture, filter screen 16 ensure that the purity of culture medium and cell mixture, graduated scale 18 facilitate control
The amount of culture medium and cell mixture, culture medium are grown for microorganism, plant tissue and animal tissue in system injection reaction bulb 3
With the nutriment of maintenance manually prepared, generally all contain carbohydrate, nitrogen substance, inorganic salts, vitamin and water etc..
When in use, culture medium and cell mixture are injected from from feed pipe 15 in culture bottle 3, seal feed pipe 15,
Entire culture bottle 3 is set to keep sealing system, the position of adjusting screw rod 10, when ensureing initial, gas bomb 8 and reaction bulb 3 are not
Connection, carbon dioxide gas is injected from gas tube 9, much has supplement sodium bicarbonate in the common buffer system of culture medium,
Sodium bicarbonate3It is easier to resolve into sodium carbonate and carbon dioxide, pH value is caused to rise, detect the bromothymol blue in bottle 17
Acid-base indicator can monitor density of carbon dioxide gas in culture bottle 3, when bromothymol blue acid-base indicator color is by indigo plant
Greening turns yellow again, it may be said that gas concentration lwevel increases in bright culture medium, sodium bicarbonate decompose the carbon dioxide that produces it is dense and
Sodium carbonate increases the pH value of culture medium, and the position of adjusting screw rod 10 makes gas vent 14 be located at outside and the reaction of gas bomb 8
The inside of bottle 3, the carbon dioxide gas for pouring gas bomb 8 in advance know from experience the inside for entering reaction bulb 3 from gas vent 14, inhibit carbonic acid
The decomposition reaction of hydrogen sodium reduces Medium's PH Value, is conducive to the adherent of cell and proliferation.
In conclusion this can inflate cell culture rolling bottle in advance, pass through gas bomb 8, gas tube 9, screw rod 10, stomata
11, gasket 12, air admission hole 13 and gas vent 14 are used cooperatively, and can in advance be poured carbon dioxide gas in gas bomb 8,
By gas bomb 8, into culture bottle 3, carbon dioxide gas adjusts the pH value of culture medium in culture bottle 3, and pH value is made to be maintained at
In the appropriate range of cell Proliferation, the adherent process of cell and cell proliferation rate are accelerated, passes through pedestal 1, supporting block 2, training
It supports bottle 3, sealing cover 4, Rotary Machine 5, rotation axis 6 and blade 7 to be used cooperatively, culture solution in culture medium can be made to become dynamic from static state
State, mixing evenly, are conducive to the adherent of cell and proliferation.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also include other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of cell culture rolling bottle that can be inflated in advance, including pedestal (1), it is characterised in that：The top of the pedestal (1)
Portion is installed with supporting block (2), and the top movable of the supporting block (2) is equipped with culture bottle (3), the culture bottle (3)
Top movable is connected with sealing cover (4), and the top of the pedestal (1), which is located on the left of culture bottle (3), is installed with support column
(19), the bottom of the support column (19) is installed with Rotary Machine (5), and the output shaft of the Rotary Machine (5) is fixedly connected with
One end is through sealing cover (4) and extends to the internal rotation axis (6) of culture bottle (3), and the surface of the rotation axis (6) is fixedly mounted
There is blade (7), is installed with gas bomb (8) on the right side of the inner wall of the culture bottle (3), the right side of the culture bottle (3) is fixed
One end is installed to sequentially pass through culture bottle (3) and gas bomb (8) and extend to the internal gas tube (9) of gas bomb (8), it is described close
It is plugged with the screw rod (10) that one end sequentially passes through sealing cover (4) and gas bomb (8) at the top of capping (4), the screw rod (10)
Top offers stomata (11), and top and stomata (11) corresponding position of the screw rod (10) are movably installed with gasket (12), institute
It states and offers air admission hole (13) on the left of screw rod (10), the left side of the screw rod (10), which is located at below air admission hole (13), to be offered
Gas vent (14).
2. a kind of cell culture rolling bottle that can be inflated in advance according to claim 1, it is characterised in that：The sealing
It is fixedly connected with feed pipe (15) at the top of lid (4), the inner wall of the feed pipe (15) is fixedly connected with filter screen (16).
3. a kind of cell culture rolling bottle that can be inflated in advance according to claim 1, it is characterised in that：The blade
(7) quantity is no less than 14, and is uniformly distributed in the surface of rotation axis (6).
4. a kind of cell culture rolling bottle that can be inflated in advance according to claim 1, it is characterised in that：The culture
It is located at below gas bomb (8) on the right side of the inner wall of bottle (3) and is installed with detection bottle (17), the inside of the detection bottle (17) is filled out
Filled with bromothymol blue acid-base indicator.
5. a kind of cell culture rolling bottle that can be inflated in advance according to claim 1, it is characterised in that：The culture
It is located on the right side of the inner wall of bottle (3) below detection bottle (17) and is installed with graduated scale (18).
6. a kind of cell culture rolling bottle that can be inflated in advance according to claim 1, it is characterised in that：The exhaust
The quantity in hole (14) is two, and the surface of the screw rod (10) is coated with orchil between two gas vents (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721842552.2U CN207699598U (en) | 2017-12-26 | 2017-12-26 | A kind of cell culture rolling bottle that can be inflated in advance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721842552.2U CN207699598U (en) | 2017-12-26 | 2017-12-26 | A kind of cell culture rolling bottle that can be inflated in advance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207699598U true CN207699598U (en) | 2018-08-07 |
Family
ID=63028699
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721842552.2U Active CN207699598U (en) | 2017-12-26 | 2017-12-26 | A kind of cell culture rolling bottle that can be inflated in advance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207699598U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518681A (en) * | 2020-05-07 | 2020-08-11 | 吉林大学 | Cell culture solution preparation facilities |
-
2017
- 2017-12-26 CN CN201721842552.2U patent/CN207699598U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518681A (en) * | 2020-05-07 | 2020-08-11 | 吉林大学 | Cell culture solution preparation facilities |
| CN111518681B (en) * | 2020-05-07 | 2023-04-07 | 吉林大学 | Cell culture solution preparation facilities |
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