CN204939469U - A kind of anerobe High pressure culture pipe - Google Patents

Abstract

The utility model discloses a kind of anerobe High pressure culture pipe, comprising: culture tube body, its both ends open, and its inwall is provided with one deck corrosion-resistant diaphragm; Access cover and outlet cap, the two ends of sealing culture tube.The utility model makes anaerobic bacteria culture liquid and High pressure culture pipe isolate by corrosion-resistant diaphragm, avoid producing the growth that corrosion product affects anerobe, high pressure conditions is in by keeping culture tube, for microorganism provides high pressure growth environment, it also avoid entrained air in testing process High pressure culture pipe, meanwhile, this device structure is simple, easy to operate, cheap, promotes extensively carrying out of anerobe research work.

Description

A kind of anerobe High pressure culture pipe

Technical field

The utility model belongs to anaerobic bacteria culture technical field, particularly relates to a kind of anerobe High pressure culture pipe.

Background technology

Anerobe is extensive in distributed in nature, and of a great variety, its effect also draws attention day by day.Anerobe be a class under anaerobic than the bacterium grown in aerobic environment, this bacterioid lack complete metabolic enzymesystem, its energy metabolism is carried out in the mode of anaerobic fermentation, therefore its cultivation should be carried out under oxygen-free environment.Existing training method has Heng Shi anaerobism rolling tube technique, anaerobic jar technology, anaerobism glove box techniques, substratum deoxygenation technology, airbag anaerobic system and anaerobic petri diss technology etc.

Anerobe in some special habitats (as oil reservoir, ocean etc.) has high voltage bearing characteristic, this kind of extreme microorganism has special genotype, metabolic mechanism and meta-bolites usually, therefore study extreme microorganism metabolic characteristics under high pressure, be beneficial to excavation and the exploitation of novel special Microbial resources and secondary metabolite thereof.

Anerobe can produce acidic substance in culturing process, and easily to existing High pressure culture pipe corrosion, and corrosion product can disturb the cultivation of anerobe, suppress the growth of anerobe; Meanwhile, in culturing process, need High pressure culture pipe liquid phase layer and gas phase layer gets appropriate amount of fluid and gas detects, get liquid and get gas process mesohigh culture tube in easy entrained air, affect the growth of anerobe; Moreover existing High pressure culture pipe complex operation, apparatus expensive, thus limit extensively carrying out of anerobe research work.

Utility model content

In order to solve the problem, the utility model provides a kind of anerobe High pressure culture pipe, by corrosion-resistant diaphragm, anaerobic bacteria culture liquid and High pressure culture pipe are isolated, avoiding producing the growth that corrosion product affects anerobe, being in high pressure conditions, for microorganism provides high pressure growth environment by keeping culture tube, it also avoid entrained air in testing process High pressure culture pipe, meanwhile, this device structure is simple, easy to operate, cheap, promotes extensively carrying out of anerobe research work.

In order to achieve the above object, the technical solution adopted in the utility model is:

A kind of anerobe High pressure culture pipe, comprising:

Culture tube body, its both ends open is also provided with outside screw at opening part, and its inwall is provided with one deck corrosion-resistant diaphragm;

Access cover and outlet cap, be arranged at the two ends of described culture tube body respectively.

Described access cover comprises:

Lid, be provided with the internal thread mated with described outside screw, and the center is provided with through hole;

Inlet pipe, its one end is communicated with described through hole;

Tensimeter, is arranged at the other end of described inlet pipe;

T-valve, be arranged between described inlet pipe and tensimeter, two interface is communicated with described inlet pipe;

Two-way valve, one end interface is by the 3rd orifice of inlet pipe and described T-valve.

Described outlet cap comprises:

Second lid, be provided with the second internal thread mated with described outside screw, and the center is provided with the second through hole;

Outlet pipe, its one end is communicated with described second through hole;

Second two-way valve, is arranged on described outlet pipe.

Further, described access cover also comprises packed-piston, is arranged between described through hole and inlet pipe.

Further, described outlet cap also comprises the second packed-piston, is arranged between described second through hole and outlet pipe.

Adopt the beneficial effect of the technical program:

The utility model makes anaerobic bacteria culture liquid and High pressure culture pipe isolate by corrosion-resistant diaphragm, avoid producing the growth that corrosion product affects anerobe, high pressure conditions is in by keeping culture tube, for microorganism provides high pressure growth environment, it also avoid entrained air in testing process High pressure culture pipe, meanwhile, this device structure is simple, easy to operate, cheap, promotes extensively carrying out of anerobe research work.

Accompanying drawing explanation

Fig. 1 is one-piece construction schematic diagram of the present utility model;

Fig. 2 is the structural representation of culture tube;

Fig. 3 is A-A sectional view in Fig. 2;

Fig. 4 is the structural representation of access cover;

Fig. 5 is the structural representation of lid;

Fig. 6 is the structural representation of the second lid.

Embodiment

In order to better understand the utility model, below in conjunction with accompanying drawing, the utility model is further elaborated.

As shown in Figure 1, a kind of anerobe High pressure culture pipe, comprising:

Culture tube body 1, its both ends open is also provided with outside screw 11 at opening part, and its inwall is provided with one deck corrosion-resistant diaphragm 12, as Fig. 2,3; Preferably, corrosion-resistant diaphragm 12 can adopt jet printing type, also can adopt detachable, so that it is changed after damaging;

Access cover 2 and outlet cap 3, be arranged at the two ends of described culture tube body 1 respectively, sealed by culture tube body 1.

As Fig. 4, described access cover 2 comprises:

Lid 21, be provided with the internal thread 211 mated with described outside screw 11, and the center is provided with through hole 212, as Fig. 5; Make lid 21 knob on culture tube body 1 by internal thread 211 and outside screw 11.

Inlet pipe 22, its one end is communicated with described through hole 212, preferably, is sealed between through hole 212 and inlet pipe 22 by packed-piston 26.

Tensimeter 23, is arranged at the other end of described inlet pipe 22, and the two interface seals, and tensimeter 23 is for measuring the air pressure level in culture tube body 1.

T-valve 24, be arranged between described inlet pipe 22 and tensimeter 23, two interface is communicated with described inlet pipe 22, and the two interface seals.

Two-way valve 25, one end interface is by three orifice of inlet pipe 22 with described T-valve 24, and the two interface seals.

Described outlet cap 3 comprises:

Second lid 31, be provided with the second internal thread 311 mated with described outside screw 11, and the center is provided with the second through hole 312, as Fig. 6; Make the second lid 31 knob on culture tube body 1 by the second internal thread 311 and outside screw 11.

Outlet pipe 32, its one end is communicated with described second through hole 312, preferably, is sealed between the second through hole 312 and outlet pipe 32 by the second packed-piston 34.

Second two-way valve 33, is arranged on described outlet pipe 32, and the two interface seals.

In order to better understand the utility model, below to using the culturing process of the utility model anerobe High pressure culture pipe to be elaborated.

Culturing process is as follows:

Step 1, open two-way valve 25 and the second two-way valve 33, pass into nitrogen from the ingress of two-way valve 25, the air of culture tube body 1 is discharged from the exit of the second two-way valve 33, after air is drained, closes two-way valve 25 and the second two-way valve 33.

Step 2, take off access cover 2, in culture tube body 1, pour appropriate anaerobic bacteria culture liquid into, cover access cover 2.

Step 3, owing to being mixed into a small amount of air in previous step, need again to pass into nitrogen with excluding air, open two-way valve 25 and the second two-way valve 33, pass into nitrogen from the ingress of two-way valve 25, the air of culture tube body 1 is discharged, after air is drained from the exit of the second two-way valve 33, close the second two-way valve 33 to continue to pass into nitrogen, make to be in high pressure conditions in culture tube body 1, until tensimeter 23 reaches scheduled pressure value, close two-way valve 25.

Step 4, the anerobe growth waited in culture tube body 1, can produce gas and the air pressure in culture tube body 1 is increased further in process of growth.

Step 5, when tensimeter 23 reaches required force value, culture tube body 1 is tilted certain angle, opens two-way valve 25, get appropriate nutrient solution, close two-way valve 25, nutrient solution is detected; Open the second two-way valve 33, owing to being in high pressure conditions in culture tube body 1, the gas in culture tube body 1 can overflow automatically, gets and trains gas in right amount, close the second two-way valve 33, detect gas; Because culture tube body 1 is in high pressure conditions, to stop in this process entrained air in culture tube body 1.

If step 6 detected result reaches required standard, then terminate to cultivate, otherwise, repeating step 4,5.

The utility model makes anaerobic bacteria culture liquid and High pressure culture pipe isolate by corrosion-resistant diaphragm 12, avoid producing the growth that corrosion product affects anerobe, high pressure conditions is in by keeping culture tube body 1, for microorganism provides high pressure growth environment, it also avoid entrained air in testing process culture tube body 1, meanwhile, this device structure is simple, easy to operate, cheap, promotes extensively carrying out of anerobe research work.

More than show and describe ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.

Claims (3)

1. an anerobe High pressure culture pipe, is characterized in that: comprising:

Culture tube body (1), its both ends open is also provided with outside screw (11) at opening part, and its inwall is provided with one deck corrosion-resistant diaphragm (12);

Access cover (2) and outlet cap (3), be arranged at the two ends of described culture tube body (1) respectively;

Described access cover (2) comprising:

Lid (21), be provided with the internal thread (211) mated with described outside screw (11), and the center is provided with through hole (212);

Inlet pipe (22), its one end is communicated with described through hole (212);

Tensimeter (23), is arranged at the other end of described inlet pipe (22);

T-valve (24), be arranged between described inlet pipe (22) and tensimeter (23), two interface is communicated with described inlet pipe (22);

Two-way valve (25), the 3rd orifice of one end interface and described T-valve (24);

Described outlet cap (3) comprising:

Second lid (31), be provided with the second internal thread (311) mated with described outside screw (11), and the center is provided with the second through hole (312);

Outlet pipe (32), its one end is communicated with described second through hole (312);

Second two-way valve (33), is arranged on described outlet pipe (32).

2. anerobe High pressure culture pipe according to claim 1, is characterized in that: described access cover (2) also comprises packed-piston (26), is arranged between described through hole (212) and inlet pipe (22).

3., according to the arbitrary described anerobe High pressure culture pipe of claim 1 or 2, it is characterized in that: described outlet cap (3) also comprises the second packed-piston (34), be arranged between described second through hole (312) and outlet pipe (32).