CN210560383U - Culture apparatus of anaerobe - Google Patents
Culture apparatus of anaerobe Download PDFInfo
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- CN210560383U CN210560383U CN201921236795.0U CN201921236795U CN210560383U CN 210560383 U CN210560383 U CN 210560383U CN 201921236795 U CN201921236795 U CN 201921236795U CN 210560383 U CN210560383 U CN 210560383U
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Abstract
The utility model relates to a culture device for anaerobic bacteria, which comprises a heat preservation water tank, a liquid storage bottle, an exhaust pipe, a liquid injection pipe, an injector, a temperature control instrument, a temperature measuring probe, a heating probe, an oxygen content detection device and an oxygen content detection probe, wherein the liquid storage bottle is arranged in the heat preservation water tank, a bottle plug is arranged at the bottle mouth of the liquid storage bottle, and the exhaust pipe and the liquid injection pipe sequentially pass through the bottle plug and extend into the liquid storage bottle; the exhaust pipe extends into the position above the liquid level of the liquid in the liquid storage bottle; the liquid injection pipe extends into the bottom of the liquid storage bottle; one side of the heat-preservation water tank is provided with a temperature control instrument which is connected with a temperature measuring probe and a heating probe, and the temperature measuring probe and the heating probe penetrate through the side wall of the heat-preservation water tank and extend into the heat-preservation water tank; the oxygen content detection probe is arranged at the bottom of the bottle plug and is connected with the oxygen content detection device through a cable penetrating through the bottle plug. The defect that the anaerobic bacteria culture device in the prior art is complex in structure and cannot provide the required gas environment in the whole process is overcome.
Description
Technical Field
The application belongs to the technical field of microbial cultivation, and particularly relates to an anaerobic bacteria culture device.
Background
The cultivation of microorganisms requires four essential basic conditions: media (i.e., nutrients), temperature, gaseous environment, and ph. Anaerobic bacteria (anaerobes) are a group of bacteria that grow better under anaerobic conditions than in aerobic environments, but not on the surface of solid media in air (18% oxygen) and/or 10% carbon dioxide concentration. With the continuous development of biotechnology, the demand of pure culture of anaerobic bacteria is increasing day by day.
At present, the culture equipment of anaerobic bacteria comprises an anaerobic bag, an anaerobic tank and an anaerobic workstation. However, the anaerobic bag and the anaerobic tank can only provide a corresponding gas environment for the sample in the culture stage, but cannot provide a gas environment in the operation stage, and are gradually eliminated due to inconvenient operation, high material cost and the like. The anaerobic workstation which can be operated and can cultivate the whole process has the defects of high purchase and use cost and overlarge occupied space.
Therefore, it is urgently needed to design a small-sized anaerobic bacteria culture apparatus which has a simple structure, occupies a small space, and can provide a required gas environment in the whole process, so as to overcome the above problems in the prior art.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a miniature anaerobe culture apparatus overcomes among the prior art anaerobe culture apparatus structure complicacy, and unable overall process provides required gaseous environment's defect. In order to achieve the purpose, the utility model adopts the technical proposal that:
a culture apparatus for anaerobic bacteria comprises a heat preservation water tank, a liquid storage bottle, an exhaust pipe, a liquid injection pipe, an injector, a temperature control instrument, a temperature measuring probe, a heating probe, an oxygen content detection device and an oxygen content detection probe, wherein the liquid storage bottle is arranged in the heat preservation water tank, a bottle plug is arranged at the bottle opening of the liquid storage bottle, and the exhaust pipe and the liquid injection pipe sequentially penetrate through the bottle plug and extend into the liquid storage bottle; the exhaust pipe extends into the position above the liquid level of the liquid in the liquid storage bottle; the liquid injection pipe extends into the bottom of the liquid storage bottle; one side of the heat-preservation water tank is provided with a temperature control instrument which is connected with a temperature measuring probe and a heating probe, and the temperature measuring probe and the heating probe penetrate through the side wall of the heat-preservation water tank and extend into the heat-preservation water tank; the oxygen content detection probe is arranged at the bottom of the bottle plug and is connected with the oxygen content detection device through a cable penetrating through the bottle plug.
Preferably, the culture device for the anaerobic bacteria further comprises an air filtering device; the air filtering device is connected with the exhaust pipe.
Preferably, the temperature measuring probe is a resistance thermometer, a thermocouple thermometer or a pressure type thermometer; the heating probe is an electromagnetic heating rod or a resistance heating rod; the temperature controller is a digital temperature controller.
Preferably, pipeline valves are arranged on the liquid injection pipe and the exhaust pipe; the exhaust pipe is connected with an air suction pump or a vacuum pump.
The utility model has the advantages that: the anaerobic bacteria can obtain sufficient nutrition by adopting the arrangement of a liquid storage bottle, an exhaust pipe, an injector and the like; the temperature control instrument is arranged on the culture medium, and the temperature control instrument is arranged on the culture medium; setting an oxygen content detection device and an oxygen content detection probe to detect the oxygen content in real time; set up aspiration pump or vacuum pump and filter equipment, discharge after the gas filtration for the waste gas in the culture apparatus discharges under the negative pressure condition like this, can not cause the pollution to the culture simultaneously. It has the advantages of low price, simplicity, convenience, practicability and controllable culture environment. Adopt the utility model discloses an anaerobe culture apparatus, the laboratory student of being convenient for practices or uses with the experimenter on a small scale, solves the problem that the producer on a small scale invested in funds. In the steps of the method, the steps of inoculating strains, extracting liquid, detecting, supplementing nutrition and the like are performed by adopting the injector, so that the operation is convenient, and the production efficiency is improved; the components of the culture solution and the activating agent are optimized, the culture solution of the utility model ensures the normal growth of the biochemical bacteria, promotes the domestication of the anaerobic bacteria, shortens the domestication time of the anaerobic bacteria and improves the biochemical treatment efficiency; the nutrient solution of the utility model improves the activity of anaerobic bacteria, promotes the reproduction of anaerobic bacteria and prevents the generation of oxygen in water. Adopt the utility model discloses an anaerobe culture apparatus, the laboratory student of being convenient for practices or uses with the experimenter on a small scale, solves the problem that the producer on a small scale invested in funds.
Drawings
FIG. 1 is a schematic structural view of the anaerobic culture apparatus of the present invention.
Fig. 2 is a schematic diagram of the circuit principle of the digital temperature controller of the present invention.
Wherein: 1. a heat preservation water tank; 2. a liquid storage bottle; 3. a bottle stopper; 4. an exhaust pipe; 5. a liquid injection pipe; 6. a pipeline valve; 7. an injector; 8. a temperature control instrument; 9. a temperature measuring probe; 10. heating the probe; 11. an oxygen content detection device; 12. an oxygen content detection probe.
Detailed Description
The following detailed description of specific embodiments of the present disclosure will be described in conjunction with the accompanying drawings to assist those skilled in the art to more fully understand the present disclosure.
Example one
A culture apparatus for anaerobic bacteria comprises a heat preservation water tank 1, a liquid storage bottle 2, an exhaust pipe 4, a liquid injection pipe 5, an injector 7, a temperature controller 8, a temperature measuring probe 9, a heating probe 10, an oxygen content detecting device 11 and an oxygen content detecting probe 12, wherein the liquid storage bottle 2 is arranged in the heat preservation water tank 1, a bottle plug 3 is arranged at the bottle mouth of the liquid storage bottle 2, and the exhaust pipe 4 and the liquid injection pipe 5 sequentially penetrate through the bottle plug 3 and extend into the liquid storage bottle 2; the exhaust pipe 4 extends into the position above the liquid level of the liquid in the liquid storage bottle 2; the liquid injection pipe 5 extends into the bottom of the liquid storage bottle 2; a temperature controller 8 is arranged on one side of the heat-preservation water tank 1, the temperature controller 8 is connected with a temperature probe 9 and a heating probe 10, and the temperature probe 9 and the heating probe 10 penetrate through the side wall of the heat-preservation water tank 1 and extend into the heat-preservation water tank 1; the oxygen content detection probe 12 is arranged at the bottom of the bottle stopper 3, and the oxygen content detection probe 12 is connected with the oxygen content detection device 11 through a cable penetrating through the bottle stopper 3.
The culture device for the anaerobic bacteria also comprises an air filtering device (not shown in the figure); the air filter device is connected to the exhaust pipe 4.
The temperature measuring probe 9 is a resistance thermometer; the heating probe 10 is an electromagnetic heating rod; the temperature controller 8 is a digital temperature controller.
Preferably, pipeline valves are arranged on the liquid injection pipe 5 and the exhaust pipe 4; the exhaust pipe 4 is connected to a suction pump (not shown).
Example two
A culture apparatus for anaerobic bacteria comprises a heat preservation water tank 1, a liquid storage bottle 2, an exhaust pipe 4, a liquid injection pipe 5, an injector 7, a temperature controller 8, a temperature measuring probe 9, a heating probe 10, an oxygen content detecting device 11 and an oxygen content detecting probe 12, wherein the liquid storage bottle 2 is arranged in the heat preservation water tank 1, a bottle plug 3 is arranged at the bottle mouth of the liquid storage bottle 2, and the exhaust pipe 4 and the liquid injection pipe 5 sequentially penetrate through the bottle plug 3 and extend into the liquid storage bottle 2; the exhaust pipe 4 extends into the position above the liquid level of the liquid in the liquid storage bottle 2; the liquid injection pipe 5 extends into the bottom of the liquid storage bottle 2; a temperature controller 8 is arranged on one side of the heat-preservation water tank 1, the temperature controller 8 is connected with a temperature probe 9 and a heating probe 10, and the temperature probe 9 and the heating probe 10 penetrate through the side wall of the heat-preservation water tank 1 and extend into the heat-preservation water tank 1; the oxygen content detection probe 12 is arranged at the bottom of the bottle stopper 3, and the oxygen content detection probe 12 is connected with the oxygen content detection device 11 through a cable penetrating through the bottle stopper 3.
The culture device for the anaerobic bacteria also comprises an air filtering device (not shown in the figure); the air filter device is connected to the exhaust pipe 4.
The temperature measuring probe 9 is a thermocouple thermometer; the heating probe 10 is a resistance heating rod; the temperature controller 8 is a digital temperature controller.
Preferably, pipeline valves are arranged on the liquid injection pipe 5 and the exhaust pipe 4; the exhaust pipe 4 is connected to a vacuum pump (not shown).
EXAMPLE III
A culture apparatus for anaerobic bacteria comprises a heat preservation water tank 1, a liquid storage bottle 2, an exhaust pipe 4, a liquid injection pipe 5, an injector 7, a temperature controller 8, a temperature measuring probe 9, a heating probe 10, an oxygen content detecting device 11 and an oxygen content detecting probe 12, wherein the liquid storage bottle 2 is arranged in the heat preservation water tank 1, a bottle plug 3 is arranged at the bottle mouth of the liquid storage bottle 2, and the exhaust pipe 4 and the liquid injection pipe 5 sequentially penetrate through the bottle plug 3 and extend into the liquid storage bottle 2; the exhaust pipe 4 extends into the position above the liquid level of the liquid in the liquid storage bottle 2; the liquid injection pipe 5 extends into the bottom of the liquid storage bottle 2; a temperature controller 8 is arranged on one side of the heat-preservation water tank 1, the temperature controller 8 is connected with a temperature probe 9 and a heating probe 10, and the temperature probe 9 and the heating probe 10 penetrate through the side wall of the heat-preservation water tank 1 and extend into the heat-preservation water tank 1; the oxygen content detection probe 12 is arranged at the bottom of the bottle stopper 3, and the oxygen content detection probe 12 is connected with the oxygen content detection device 11 through a cable penetrating through the bottle stopper 3.
The culture device for the anaerobic bacteria also comprises an air filtering device (not shown in the figure); the air filter device is connected to the exhaust pipe 4.
The temperature measuring probe 9 is a pressure type temperature measuring meter; the heating probe 10 is a resistance heating rod; the temperature controller 8 is a digital temperature controller.
Preferably, the liquid injection pipe 5 and the exhaust pipe 4 are both provided with pipeline valves 6; the exhaust pipe 4 is connected to a vacuum pump (not shown).
The method for culturing the anaerobic bacteria by adopting the anaerobic bacteria culture device can shorten the domestication culture time of the anaerobic bacteria and provide high biochemical treatment efficiency, and comprises the following steps:
(1) sterilizing the heat-preservation water tank, the liquid storage bottle, the exhaust pipe, the liquid injection pipe, the injector, the temperature measuring probe and the heating probe;
(2) preparing a culture strain, and inoculating the culture strain subjected to pseudo-anaerobic culture for later use;
(3) after the fermentation culture solution is added into the liquid storage bottle, a bottle plug communicated with an exhaust pipe and a liquid injection pipe is covered; ensuring that the exhaust pipe extends into the position above the liquid level of the liquid in the liquid storage bottle; the liquid injection pipe extends into the bottom of the liquid storage bottle;
(4) injecting the culture specimen into the fermentation culture solution by using an injector; injecting nutrient solution into the fermentation culture solution; extracting a culture solution containing anaerobic bacteria to detect the pH value of the culture solution;
(5) the heating probe is controlled by the temperature controller to heat according to the preset temperature, and the real-time temperature measured by the temperature measuring probe is adjusted;
(6) air in the liquid storage bottle is discharged through an exhaust pipe by adopting an air suction pump or a vacuum pump;
(7) and the temperature controller displays that the temperature reaches the preset temperature and then carries out constant-temperature timing culture.
The nutrient solution comprises the following components in parts by weight: 30 parts of sodium chloride, 20 parts of ammonium chloride, 10 parts of magnesium sulfate, 5 parts of monopotassium phosphate, 20 parts of morpholine ethanesulfonic acid and 10 parts of yeast extract.
The fermentation culture solution comprises the following components in parts by weight: 10 parts of glucose, 20 parts of sodium chloride, 10 parts of penicillin, 5 parts of aspergillus niger, 5 parts of dipotassium hydrogen phosphate, 10 parts of zinc sulfate, 10 parts of ammonium sulfate, 5 parts of citric acid, 10 parts of yeast extract and 5 parts of morphine ethanesulfonic acid.
The culture conditions were: the preset temperature is 30-50 ℃, the PH value is 3-7, and the time is 50-70 hours.
The filtering device comprises three-stage filtering, wherein the first-stage filtering is a sterilized absorbent cotton barrel, and the second-stage filtering and the third-stage filtering are sterilized absorbent cotton rods or balls.
While the preferred embodiments of the present invention have been described, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the general inventive concept, and it is intended to cover all such changes and modifications as fall within the true spirit and scope of the invention.
Claims (4)
1. A culture apparatus of anaerobic bacteria comprises a heat preservation water tank, a liquid storage bottle, an exhaust pipe, a liquid injection pipe, an injector, a temperature control instrument, a temperature measuring probe, a heating probe, an oxygen content detection device and an oxygen content detection probe, and is characterized in that the liquid storage bottle is arranged in the heat preservation water tank, a bottle plug is arranged at the bottle mouth of the liquid storage bottle, and the exhaust pipe and the liquid injection pipe sequentially penetrate through the bottle plug and extend into the liquid storage bottle; the exhaust pipe extends into the position above the liquid level of the liquid in the liquid storage bottle; the liquid injection pipe extends into the bottom of the liquid storage bottle; one side of the heat-preservation water tank is provided with a temperature control instrument which is connected with a temperature measuring probe and a heating probe, and the temperature measuring probe and the heating probe penetrate through the side wall of the heat-preservation water tank and extend into the heat-preservation water tank; the oxygen content detection probe is arranged at the bottom of the bottle plug and is connected with the oxygen content detection device through a cable penetrating through the bottle plug.
2. The culture apparatus of anaerobic bacteria according to claim 1, further comprising an air filtering device; the air filtering device is connected with the exhaust pipe.
3. The apparatus for culturing anaerobic bacteria according to claim 1, wherein the temperature probe is a resistance thermometer, a thermocouple thermometer, or a pressure thermometer; the heating probe is an electromagnetic heating rod or a resistance heating rod; the temperature controller is a digital temperature controller.
4. The culture apparatus for anaerobic bacteria according to claim 1, wherein the liquid injection pipe and the exhaust pipe are provided with pipeline valves; the exhaust pipe is connected with an air suction pump or a vacuum pump.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921236795.0U CN210560383U (en) | 2019-08-01 | 2019-08-01 | Culture apparatus of anaerobe |
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| CN201921236795.0U CN210560383U (en) | 2019-08-01 | 2019-08-01 | Culture apparatus of anaerobe |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110437973A (en) * | 2019-08-01 | 2019-11-12 | 山东农微生物科技有限公司 | A kind of culture apparatus and cultural method of anaerobic bacteria |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110437973A (en) * | 2019-08-01 | 2019-11-12 | 山东农微生物科技有限公司 | A kind of culture apparatus and cultural method of anaerobic bacteria |
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