CN208869602U - A kind of bioreactor perfusion culture plumbing connection system - Google Patents
A kind of bioreactor perfusion culture plumbing connection system Download PDFInfo
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- CN208869602U CN208869602U CN201821119041.2U CN201821119041U CN208869602U CN 208869602 U CN208869602 U CN 208869602U CN 201821119041 U CN201821119041 U CN 201821119041U CN 208869602 U CN208869602 U CN 208869602U
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- bioreactor
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- perfusion culture
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Abstract
The utility model discloses a kind of bioreactor perfusion culture plumbing connection systems, system is connected with a set of liquid outlet pipe including a set of input duct connection system, the input duct connection system has the first four-way connection, and liquid outlet pipe, which connects system, has the second four-way connection.The bioreactor perfusion culture plumbing connection system of the utility model can realize bioreactor perfusion culture, it can also fast quick change liquid, the pipeline of bioreactor perfusion culture plumbing connection system is convenient for cleaning, it can repeatedly use, production cost has been saved, pollution risk can also be reduced and has improved working efficiency.The utility model carries out the production operation of bioreactor instead of existing single pipeline, the sterility requirements in production have not only been effectively ensured, and it is easy to operate, the accurate perfusion of bioreactor perfusion culture can be achieved, it can also realize and quickly change liquid operation, sterile working needed for greatly reducing pipeline connection, to reduce pollution risk and improve work efficiency.
Description
Technical field
The utility model relates to the technical fields of bio-pharmaceuticals more particularly to a kind of bioreactor perfusion culture pipeline to connect
Welding system.
Background technique
Traditional most of bioreactor culture is fed-batch culture in bio-pharmaceutical industry, but in recent years, it is right
The interest of continuous processing is being continuously increased.Compared with batch or fed-batch culture, perfusion culture advantage is to give cell more
Good growing environment, to reach more preferable cell state, higher cell density and corresponding higher unit-protein yield.This
It has the advantage that, perfusion culture can obtain the purpose product of more an order of magnitude compared with fed-batch culture.But perfusion
Culture needs to use a large amount of culture solution and generates a large amount of culture waste liquid and harvest liquid, and the replacement of these solution is to sterile behaviour
Bring biggish challenge, it is therefore desirable to design a set of bioreactor perfusion culture plumbing connection system to simplify related behaviour
Make and reduce pollution risk.
Utility model content
In view of the deficiencies of the prior art, the technical problem to be solved by the utility model is to provide a kind of bioreactors
Perfusion culture plumbing connection system not only can be reduced sterile working, but also can reduce pollution risk.
In order to solve the above-mentioned technical problem, the utility model is achieved through the following technical solutions: a kind of bioreactor
Perfusion culture plumbing connection system, including a set of input duct connection system connect system, the feed liquor with a set of liquid outlet pipe
Plumbing connection system has the first four-way connection；The first port of first four-way connection by perfusion pump line and silicone tube group
Conjunction is connect with the first liquid feed valve of bioreactor, and the second port of first four-way connection is by feed liquor pump line and silicone tube
Combination is connect with the second liquid feed valve of the bioreactor, and the third port of first four-way connection passes through silicone tube and training
The outlet end of nutrient solution bag connects, and the 4th port of first four-way connection is as standby port；Liquid outlet pipe connection system
System has the second four-way connection；The first port of second four-way connection is anti-by the combination of perfusion pump line and silicone tube and biology
Answer the liquid valve on device top to connect, the second port of second four-way connection by Pump for giving-out pipe and silicone tube combination with it is described
The tapping valve at bioreactor tank bottom end connects, the third port of second four-way connection by silicone tube and waste fluid bag into
The connection of liquid end, the 4th port of second four-way connection is as standby port.
Preferably, the 4th port of first four-way connection connects cell suspension or another culture solution bag by silicone tube
Outlet end；4th port of second four-way connection is connected by silicone tube with the liquid feeding end of another waste fluid bag.
Further, configured with accurate peristaltic pump on the perfusion pump line, for carrying out the perfusion culture of bioreactor.
Further, it is configured with industrial peristaltic pump on the feed liquor pump line and Pump for giving-out pipe, for carrying out bioreactor
Change liquid operation.
In an embodiment of the utility model, each port of first four-way connection and the second four-way connection
It is designed with flow-stopping clip.
The bioreactor perfusion culture plumbing connection system of the utility model at least has the following technical effect that practical
Novel bioreactor perfusion culture plumbing connection system can realize bioreactor perfusion culture, can also fast quick change liquid, it is raw
The pipeline of object reactor perfusion culture plumbing connection system is convenient for cleaning, can repeatedly use, save production cost, can also
To reduce pollution risk and improve working efficiency.In addition, the utility model proposes bioreactor perfusion culture pipeline connection
System carries out the production operation of bioreactor instead of existing single pipeline, has not only been effectively ensured sterile in production
It is required that and it is easy to operate, it can be achieved that bioreactor perfusion culture accurate perfusion, also may be implemented quickly to change liquid behaviour
Make, sterile working needed for greatly reducing pipeline connection, to reduce pollution risk and improve work efficiency.
Detailed description of the invention
In order to illustrate more clearly of the technical solution of the utility model embodiment, the attached drawing of embodiment will be made below simple
Ground introduction.
Fig. 1 is the structural representation of the bioreactor perfusion culture plumbing connection system of the preferred embodiment in the utility model
Figure.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in Figure 1, being the knot of the bioreactor perfusion culture plumbing connection system of the preferred embodiment in the utility model
Structure schematic diagram, the bioreactor perfusion culture plumbing connection system of the utility model include a set of input duct connection system 1
System 2 is connected with a set of liquid outlet pipe, the input duct connection system 1 has the first four-way connection 10, and the described 1st
The first port of pass joint 10 is connected by the combination of perfusion pump line 11 and silicone tube and the first liquid feed valve of bioreactor, described
Combination and second feed liquor of the bioreactor of the second port of first four-way connection 10 by feed liquor pump line 12 and silicone tube
The third port of valve connection, first four-way connection 10 is connected by silicone tube 13 with the outlet end of culture solution bag, and the one or four
4th port of pass joint 10 can be connected thin as standby port 14, the standby port 14 of the first four-way connection 10 by silicone tube
The outlet end of born of the same parents' suspension or another culture solution bag.
The liquid outlet pipe connection system 2 of the utility model has the second four-way connection 20, and second four-way connection 20
First port connect by the combination of perfusion pump line 11 and silicone tube with the liquid valve on bioreactor top, second four-way
The second port of connector 20 is connect by the combination of Pump for giving-out pipe 22 and silicone tube with the tapping valve at bioreactor tank bottom end,
The third port of second four-way connection 20 is connected by silicone tube 13 with the liquid feeding end of waste fluid bag, second four-way connection
20 the 4th port can pass through silicone tube and another waste liquid as standby port 14, the standby port 14 of the second four-way connection 20
The liquid feeding end connection of bag.
Wherein, the perfusion pump line 11 configures the perfusion culture that accurate peristaltic pump carries out bioreactor, feed liquor pump line 12
It is operated with the liquid that changes for configuring industrial peristaltic pump progress bioreactor on Pump for giving-out pipe 22.Certainly, in the present invention, liquid out
Pump line 22 can not also pump out liquid by industry wriggling, and directly go out liquid by pressure inside the tank.The first four-way in the utility model
Each port of connector 10 and the second four-way connection 20 is designed with flow-stopping clip, clamps pipeline using flow-stopping clip, realizes to pipeline
It is closed.
Embodiment:
As shown in Figure 1, the utility model input duct connection system 1 the first four-way connection 10 first port by
The combination of the 73# silicone tube of the 25# perfusion pump line 11 and Nalgene of Cole-Parmer has with the safe medical equipment engineering in Shanghai day
First liquid feed valve of the 09 50L bioreactor of Cellipower of limit company connects, the second port of the first four-way connection 10
By the safe medical equipment engineering of the combination of the 73# silicone tube of the 73# feed liquor pump line 12 and Nalgene of Cole-Parmer and Shanghai day
Second liquid feed valve of the 09 50L bioreactor of Cellipower of Co., Ltd connects, the third end of the first four-way connection 10
Mouth is connected by the 73# silicone tube 13 of Nalgene with newborn bovine serum culture solution outlet end, the 4th port of the first four-way connection 10
It is connect by the 73# silicone tube 13 of Nalgene with the outlet end of another bag of newborn bovine serum culture solution.
When carrying out bioreactor perfusion culture feed liquor, the first port and third port of the first four-way connection 10 are opened
The flow-stopping clip at place, while starting Baoding LanGe constant flow pump Co., Ltd at the 25# perfusion pump line 11 of Cole-Parmer
BT100-2J precision peristaltic pump can carry out perfusion culture feed liquor to the pump speed needed.
When progress bioreactor changes the feed liquor operation of liquid, second port and the third end of the first four-way connection 10 are opened
Flow-stopping clip at mouthful, while starting Baoding LanGe constant flow pump Co., Ltd at the 73# feed liquor pump line 12 of Cole-Parmer
YT600-1J industry peristaltic pump can carry out changing the feed liquor operation of liquid to the pump speed needed.
The first port of second four-way connection 20 of the liquid outlet pipe connection system 2 of the utility model is by Cole-Parmer
25# perfusion pump line 11 and Nalgene 73# silicone tube combination with Shanghai Ritai Medical Equipment Engineering Co., Ltd.
The liquid valve of 09 50L bioreactor upper top cover of Cellipower connects, and the second port of the second four-way connection 20 is by Cole-
The combination of the 73# silicone tube of the 73# Pump for giving-out pipe 22 and Nalgene of Parmer and Shanghai Ritai Medical Equipment Engineering Co., Ltd.
09 50L bioreactor tank bottom of Cellipower tapping valve connection, the third port of the second four-way connection 20 by
The 73# silicone tube 13 of Nalgene is connected with the liquid feeding end of waste fluid bag, and the 4th port of the second four-way connection 20 is by Nalgene's
73# silicone tube 13 is connected with the liquid feeding end of another waste fluid bag.
When progress bioreactor perfusion turns out liquid, the first port and third port of the second four-way connection 20 are opened
The flow-stopping clip at place, while starting Baoding LanGe constant flow pump Co., Ltd at the 25# perfusion pump line 11 of Cole-Parmer
BT100-2J precision peristaltic pump can carry out perfusion to the pump speed needed and turn out liquid.
When going out liquid operation of liquid is changed when carrying out bioreactor, opens second port and the third end of the second four-way connection 20
Flow-stopping clip at mouthful, while starting Baoding LanGe constant flow pump Co., Ltd at the 73# Pump for giving-out pipe 22 of Cole-Parmer
YT600-1J industry peristaltic pump is operated to the liquid that goes out that the pump speed needed can carry out changing liquid.
It carries out bioreactor and changes another scheme for going out liquid operation of liquid to be to open the second end of the second four-way connection 20
Flow-stopping clip at mouth and third port, improving bioreactor gas flow makes to generate enough positive pressures in tank, is made by pressure
Second port and third port that solution in tank passes sequentially through tank bottom tapping valve, the second four-way connection 20 are obtained, into waste fluid bag.
The bioreactor perfusion culture plumbing connection system of the utility model, it can be achieved that bioreactor perfusion culture,
Can also fast quick change liquid, the pipeline of bioreactor perfusion culture plumbing connection system is convenient for cleaning, can repeatedly use, and saves
Production cost can also reduce pollution risk and improve working efficiency.In addition, the utility model proposes bioreactor filling
Stream culture plumbing connection system, the production operation of bioreactor is carried out instead of existing single pipeline, is not only effectively ensured
Sterility requirements in production, and it is easy to operate, it can be achieved that bioreactor perfusion culture accurate perfusion, also may be implemented
Liquid operation is quickly changed, sterile working needed for greatly reducing pipeline connection, to reduce pollution risk and improve work
Make efficiency.
Above disclosed is only the preferred embodiment of the utility model, certainly cannot be practical new to limit this with this
The interest field of type, therefore equivalent variations made according to the claim of the utility model still belong to the model that the utility model is covered
It encloses.
Claims (5)
1. a kind of bioreactor perfusion culture plumbing connection system, it is characterised in that: connect system including a set of input duct
System is connected with a set of liquid outlet pipe, the input duct connection system has the first four-way connection；First four-way connection
First port by perfusion pump line and silicone tube combination and bioreactor the first liquid feed valve connect, the described 1st connects
The second port of head is connect by the combination of feed liquor pump line and silicone tube with the second liquid feed valve of the bioreactor, and described first
The third port of four-way connection is connected by silicone tube with the outlet end of culture solution bag, the 4th port of first four-way connection
As standby port；The liquid outlet pipe connection system has the second four-way connection；The first port of second four-way connection
It is connect by the combination of perfusion pump line and silicone tube with the liquid valve on bioreactor top, the second end of second four-way connection
Mouth is connect by the combination of Pump for giving-out pipe and silicone tube with the tapping valve at bioreactor tank bottom end, second four-way connection
Third port connected with the liquid feeding end of waste fluid bag by silicone tube, the 4th port of second four-way connection is as spare end
Mouthful.
2. bioreactor perfusion culture plumbing connection system as described in claim 1, it is characterised in that: first four-way
4th port of connector connects the outlet end of cell suspension or another culture solution bag by silicone tube；Second four-way connection
4th port is connected by silicone tube with the liquid feeding end of another waste fluid bag.
3. bioreactor perfusion culture plumbing connection system as described in claim 1, it is characterised in that: the perfusion pump line
It is upper to be configured with accurate peristaltic pump, for carrying out the perfusion culture of bioreactor.
4. bioreactor perfusion culture plumbing connection system as described in claim 1, it is characterised in that: the feed liquor pump line
With configured with industrial peristaltic pump, change liquid operation for carry out bioreactor on Pump for giving-out pipe.
5. bioreactor perfusion culture plumbing connection system as described in claim 1, it is characterised in that: first four-way
Each port of connector and the second four-way connection is designed with flow-stopping clip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821119041.2U CN208869602U (en) | 2018-07-13 | 2018-07-13 | A kind of bioreactor perfusion culture plumbing connection system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821119041.2U CN208869602U (en) | 2018-07-13 | 2018-07-13 | A kind of bioreactor perfusion culture plumbing connection system |
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| Publication Number | Publication Date |
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| CN208869602U true CN208869602U (en) | 2019-05-17 |
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| CN201821119041.2U Active CN208869602U (en) | 2018-07-13 | 2018-07-13 | A kind of bioreactor perfusion culture plumbing connection system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108977358A (en) * | 2018-07-13 | 2018-12-11 | 广州诺诚生物制品股份有限公司 | A kind of closed bioreactor and its cell culture processes |
-
2018
- 2018-07-13 CN CN201821119041.2U patent/CN208869602U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108977358A (en) * | 2018-07-13 | 2018-12-11 | 广州诺诚生物制品股份有限公司 | A kind of closed bioreactor and its cell culture processes |
| CN108977358B (en) * | 2018-07-13 | 2022-03-25 | 广州诺诚生物制品股份有限公司 | Closed bioreactor and cell culture method thereof |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 510000 No. 406, Lianyun Road, economic and Technological Development Zone, Guangzhou, Guangdong Patentee after: Guangzhou Baiyunshan Biological Products Co.,Ltd. Address before: 510530 No. 406, Lianyun Road, Guangzhou Economic and Technological Development Zone, Guangdong Province Patentee before: GUANGZHOU PROMISE BIOLOGICAL PRODUCTS Co.,Ltd. |
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| CP03 | Change of name, title or address |