CN201148433Y - Immersion type film bioreactor - Google Patents
Immersion type film bioreactor Download PDFInfo
- Publication number
- CN201148433Y CN201148433Y CN 200720187344 CN200720187344U CN201148433Y CN 201148433 Y CN201148433 Y CN 201148433Y CN 200720187344 CN200720187344 CN 200720187344 CN 200720187344 U CN200720187344 U CN 200720187344U CN 201148433 Y CN201148433 Y CN 201148433Y
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- CN
- China
- Prior art keywords
- membrane
- hollow fiber
- bioreactor
- air
- reactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/16—Hollow fibers
Abstract
The utility model relates to an immersive type membrane bioreactor that adopts a membrane component to realize the on-line separation of products. The bioreactor is mainly used for generating and separating the secondary metabolites of high additional value in the growth and metabolism process of the caterpillar fungus and other microorganisms, and belongs to the technological field of pure microorganism breeding and membrane separation. The reactor comprises a fermentation cylinder, an air distributor, a draft tube and a hollow fiber membrane component. The bottom of the fermentation cylinder is provided with a sealed outlet that is connected with the port of the hollow fiber membrane, and is connected with a vacuum pump through a pipeline; the hollow fiber membrane component is dynamically arranged on the bottom of the fermentation cylinder; the air distributor is arranged under the hollow fiber membrane component; the membrane component is a polymer separation membrane. The membrane bioreactor can be used in the field of continuous pure breeding of organisms, in particular in the field of aerobic cells. And the membrane bioreactor has the advantages of small occupied area, high density of cell fermentation and high conversion rate of the products.
Description
Technical field
The utility model relates to a kind of original position and separates one film biological reactor---submerged membrane bioreactor, belongs to the crossing domain of microorganism pure culture and membrane separation technique.
Background technology
It is two principal elements of limit production intensity and production concentration in the biological respinse that product and substrate suppress.Because product and substrate suppress, the potentiality of biomass cells and enzyme can not be given full play to, and cause the transformation efficiency (or productive rate) of reaction process also to be difficult to improve.For example in cell cultivation process, be accompanied by the accumulation of products of cellular metabolism, cell growth can produce feedback inhibition, causes that the meta-bolites generating rate reduces, thereby has reduced the output of metabolite.Have aspect the modern production of the anticancer newtype drug of Mycophyta of high economic worth having the medicinal fungi Cordyceps sinensis of China's characteristic, Phellinus, glossy ganoderma etc.; Cordyceps polysaccharide, Phellinus polysaccharide and the contour value-added product of ganoderan are the secondary metabolites in the microorganism growth metabolic process, exist in the batch fermentation process later stage and utilize phenomenon again.
For a long time, the approach that always addresses this problem of people in exploration.Traditional bio-reactor mainly comprises airlift reactor, mechanical stir-reactor, immobilized enzyme reactor and solid-state fermentation reactor, but above-mentioned reactor only is biological respinse to be regulated and control device, and the separation of product will be by method realizations such as follow-up biological separating unit filtration, distillation, centrifugal, recrystallizations.Immobilized enzyme membrane type reactor is a kind of reactor that enzyme immobilization is constituted on film.Make composite membrane sticking together with the film (enzyme membrane) of enzyme and a semipolar linkage.Semipolar linkage one side contacts with raw material, and substrate can optionally be reacted by entering enzyme membrane behind the semipolar linkage, and reactor product goes out from enzyme membrane one effluent.Product is constantly separated from reactive system.Substrate is to lean on the pressure reduction of film both sides to pass through film.So the concentration of substrate and product is all lower on enzyme membrane, being particularly conducive to substrate and product has inhibiting reaction.
Membrane bioreactor (MBR) is the novel water technology that a kind of film separation unit combines with biological processing unit, entered the practicability stage, membrane bioreactor can not only and can be trapped in macromolecular substance in the reactor all cells, can keep the biomass of high density in the reactor.The utility model is introduced the fermentation field with hollow-fibre membrane thus, adopt membrane bioreactor system constantly cell to be separated with product, the accumulation of control product, effectively prevent the consumption of polysaccharide product or tunnings such as ethanol, lactic acid feedback inhibition to bacterial classification, solved problems such as product suppresses in the cell growth process, substrate is selected supply, the metabolite productive rate is low, fermented liquid complicated component separation difficulty, realization cell high-density is continuously fermented.
The utility model content
The purpose of this utility model is to solve problems such as product suppresses in the cell growth process, substrate is selected supply, the metabolite productive rate is low, fermented liquid complicated component separation difficulty, provides a kind of membrane module that utilizes to carry out the isolating integral type film biological reactor of original position.Its compact construction, energy consumption are low and can reduce film significantly and pollute, thereby can realize littler floor space, lower cost and realize the yield of higher target product.
A kind of submerged membrane bioreactor that the utility model provides is characterized in that: it contains biological culture jar 1, inlet mouth 2, air outlet 3, guide shell 4, hollow fiber film assembly 5, biological culture jar outlet 6, hollow fiber film assembly port 7, air-distributor 8, fermentation tank culture medium inlet 9; Described guide shell 4, air-distributor 8 and membrane module 5 are built in biological culture jar 1 inside; Described membrane module 5 is a hollow-fibre membrane, and it is built in biological culture jar 1 bottom, and is positioned at air-distributor 8 tops, and an end opening is connected with the reactor exterior line by gasket.
In above-mentioned submerged membrane bioreactor, described hollow fiber film assembly 5 is for dynamic annular microfiltration membrane or hyperfiltration membrane assembly and can tolerate 115 ℃ of high temperature, membrane pore size is 0.01-0.3um, membrane module is positioned at the inside reactor lower end, and hollow fiber film assembly port 7 is by silicagel pad and biological culture outlet 6 sealings.
In above-mentioned submerged membrane bioreactor, described guide shell 4 is one to be fixed in the framework in the reactor, and described hollow fiber film assembly 5 is around guide shell 4 lower ends; Described air-distributor 8 is an annular air sparger, is positioned at below the membrane module, and its diameter is identical with round membrane assembly 5 diameters.
In above-mentioned submerged membrane bioreactor, described membrane bioreactor is an airlift bioreactor, and oxygen is supplied with by the annular air sparger 8 of reactor bottom.
Submerged membrane bioreactor of the present utility model is made up of biological culture jar, guide shell, air-distributor and ring-like membrane module; Guide shell, air-distributor and membrane module are built in biological culture jar inside, and membrane module is a hollow-fibre membrane, place air-distributor top, and an end opening is connected with the reactor exterior line by gasket, and obtain clarified broth by the vacuum pump suction.
Because submerged membrane bioreactor provided by the invention, whole reactor and membrane module adopt the high-temperature heat sterilization operation to carry out, guaranteed microorganism pure culture requirement, the generation that has well improved the secondary metabolite of high added value in the microorganism growth metabolic processes such as Cordyceps sinensis with separate.The utility model can be used for biological pure culture field continuously, especially aerobic field of cell culture, and it is little to have floor space, high-density cells fermentation, the advantage that product yield is high.
Description of drawings
Fig. 1 is a kind of submerged membrane bioreactor sectional view that is applied in the biological pure culture field described in the utility model;
Among the figure: 1, biological culture jar, 2, inlet mouth, 3, the air outlet, 4, guide shell, 5, hollow fiber film assembly, 6, the outlet of biological culture jar, 7, the hollow-fibre membrane port, 8, air-distributor, 9, the fermentation tank culture medium inlet.
Embodiment:
Referring to Fig. 1, a kind of submerged membrane bioreactor that is applied to microorganism pure culture field described in the utility model comprises biological culture jar (1), guide shell (4), air-distributor (8) and ring-like membrane module (5).Hollow fiber film assembly (5) places the fermentor tank bottom, above the air-distributor (8), when apparatus working, when the process hollow-fibre membrane is surperficial from the bubble uphill process of air-distributor ejection, bubble breaks, produce great shear forces, thereby Dynamic Membrane is washed away, thereby delay the pollution of film.Seal by silicagel pad between hollow-fibre membrane port (7) and the biological culture jar outlet (6), and be connected with vacuum pump by pipeline.
Assemble membrane bioreactor (5L), reactor is sterilized, 115 ℃ of sterilizing parameters, 30min inserts seed fermentation liquid then and carries out the microorganism pure culture.After fermentation proceeds to certain hour, open peristaltic pump, constantly stream adds aseptic culture medium in reactor by fermentation tank culture medium inlet (9); Open vacuum pump simultaneously vacuum tightness 0.02MPa is set, handle secondary fermentation liquid by fermentor tank outlet (6) membrane that continues to flow.Fermented liquid turbidity 0.49NTU, the backwashing period of hollow fiber film assembly (5) reaches 100h.Through the effusive fermented liquid of this membrane bioreactor, need not any pre-treatment, can be directly used in operations such as purification of products.
Claims (4)
1, a kind of submerged membrane bioreactor, it is characterized in that: it contains biological culture jar (1), inlet mouth (2), air outlet (3), guide shell (4), hollow fiber film assembly (5), biological culture jar outlet (6), hollow fiber film assembly port (7), air-distributor (8), fermentation tank culture medium inlet (9); Described guide shell (4), air-distributor (8) and membrane module (5) are built in biological culture jar (1) inside; Described membrane module (5) is a hollow-fibre membrane, and it is built in biological culture jar (1) bottom, and is positioned at air-distributor (8) top, and an end opening is connected with the reactor exterior line by gasket.
2, submerged membrane bioreactor according to claim 1, it is characterized in that: described hollow fiber film assembly (5) is for dynamic annular microfiltration membrane or hyperfiltration membrane assembly and can tolerate 115 ℃ of high temperature, membrane pore size is 0.01-0.3um, membrane module is positioned at the inside reactor lower end, and hollow fiber film assembly port (7) is by silicagel pad and biological culture outlet (6) sealing.
3, submerged membrane bioreactor according to claim 1 is characterized in that: described guide shell (4) is one to be fixed in the framework in the reactor, and described hollow fiber film assembly (5) is around guide shell (4) lower end; Described air-distributor (8) is an annular air sparger, is positioned at below the membrane module, and its diameter is identical with round membrane assembly (5) diameter.
4, submerged membrane bioreactor according to claim 1 is characterized in that: described membrane bioreactor is an airlift bioreactor, and oxygen is supplied with by the annular air sparger (8) of reactor bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720187344 CN201148433Y (en) | 2007-12-21 | 2007-12-21 | Immersion type film bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720187344 CN201148433Y (en) | 2007-12-21 | 2007-12-21 | Immersion type film bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201148433Y true CN201148433Y (en) | 2008-11-12 |
Family
ID=40116200
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200720187344 Expired - Fee Related CN201148433Y (en) | 2007-12-21 | 2007-12-21 | Immersion type film bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201148433Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865792A (en) * | 2014-03-28 | 2014-06-18 | 济南大学 | Circulating microbial fermentation reaction and feed liquid separation integrated equipment |
| CN107794219A (en) * | 2016-08-30 | 2018-03-13 | 上海吉态来生物技术有限公司 | A kind of bioreactor for fermentation of gaseous substrates |
-
2007
- 2007-12-21 CN CN 200720187344 patent/CN201148433Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865792A (en) * | 2014-03-28 | 2014-06-18 | 济南大学 | Circulating microbial fermentation reaction and feed liquid separation integrated equipment |
| CN103865792B (en) * | 2014-03-28 | 2016-02-03 | 济南大学 | A kind of circulating fermentable reaction and feed liquid isolation integral equipment |
| CN107794219A (en) * | 2016-08-30 | 2018-03-13 | 上海吉态来生物技术有限公司 | A kind of bioreactor for fermentation of gaseous substrates |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081112 Termination date: 20151221 |
|
| EXPY | Termination of patent right or utility model |