CN201148433Y - A submerged membrane bioreactor - Google Patents

Abstract

The utility model relates to a submerged membrane bioreactor, which utilizes membrane components to separate products online, and is mainly used to improve the generation and separation of secondary metabolites with high added value in the growth and metabolism process of microorganisms such as Cordyceps sinensis. The invention belongs to the technical field of microorganism pure culture and membrane separation technology. The reactor includes a fermenter, an air distributor, a draft tube, and a hollow fiber membrane module. The bottom of the fermenter has an outlet that is connected to the port of the hollow fiber membrane and is sealed, and is connected to a vacuum pump through a pipeline; the hollow fiber membrane module is dynamically placed at the bottom of the fermenter, with an air distributor below it, and the membrane module is used for polymer separation. membrane. The utility model can be used in the field of continuous biological pure culture, especially in the field of aerobic cell culture, and has the advantages of small footprint, high-density cell fermentation and high product conversion rate.

Description

A submerged membrane bioreactor

technical field

The utility model relates to an in-situ separation integrated membrane bioreactor—a submerged membrane bioreactor, which belongs to the intersecting field of microbial pure culture and membrane separation technology.

Background technique

Product and substrate inhibition are the two major factors limiting production intensity and product concentration in biological reactions. Due to the inhibition of products and substrates, the potential of biological cells and enzymes cannot be fully exerted, which makes it difficult to increase the conversion rate (or yield) of the reaction process. For example, in the process of cell culture, with the accumulation of cell metabolites, there will be a feedback inhibition effect on cell growth, resulting in a decrease in the rate of metabolite production, thereby reducing the yield of metabolites. In the modern production of new fungal anti-cancer drugs with high economic value, such as medicinal fungi Cordyceps sinensis, Phellinus, and Ganoderma lucidum with Chinese characteristics, high value-added products such as Cordyceps polysaccharides, Phellinus polysaccharides, and Ganoderma lucidum polysaccharides are the growth and metabolism of microorganisms. The secondary metabolites in the batch fermentation process are reused in the later stage of the batch fermentation process.

For a long time, people have been exploring ways to solve this problem. Traditional bioreactors mainly include airlift reactors, mechanically stirred reactors, immobilized enzyme reactors and solid-state fermentation reactors, but the above-mentioned reactors are only a biological reaction adjustment and control device, and the separation of products must be carried out through subsequent processes. The bio-separation unit is realized by methods such as filtration, distillation, centrifugation, and recrystallization. The immobilized enzyme membrane reactor is a reactor composed of immobilized enzymes on a membrane. The membrane with enzyme (enzyme membrane) and the semi-permeable membrane are pasted together to form a composite membrane. One side of the semipermeable membrane is in contact with the raw material, so that the substrate can selectively pass through the semipermeable membrane and then enter the enzyme membrane for reaction, and the reaction product flows out from the side of the enzyme membrane. The product can be continuously separated from the reaction system. The substrate is passed through the membrane by the pressure difference across the membrane. Therefore, the concentrations of substrates and products on the enzyme membrane are relatively low, which is especially beneficial to reactions where substrates and products have inhibitory effects.

Membrane bioreactor (MBR) is a new type of water treatment technology that combines membrane separation unit and biological treatment unit. It has entered the practical stage. Membrane bioreactor can not only trap all cells but also macromolecular substances In the reactor, it can maintain a high concentration of bacteria in the reactor. Therefore, the utility model introduces the hollow fiber membrane into the fermentation field, adopts the membrane bioreactor system to continuously separate the cells and products, controls the accumulation of products, and effectively prevents the consumption of polysaccharide products or the feedback inhibition of bacteria by fermentation products such as ethanol and lactic acid It solves the problems of product inhibition, substrate selective supply, low yield of metabolites, and difficult separation of complex components of fermentation broth during cell growth, and realizes high-density continuous fermentation of cells.

Utility model content

The purpose of this utility model is to solve the problems of product inhibition, substrate selective supply, low yield of metabolites, and difficult separation of fermentation liquid components in the process of cell growth, and provide an integrated membrane bioreactor that uses membrane components for in-situ separation . It has compact structure, low energy consumption and can greatly reduce membrane fouling, so that it can achieve smaller footprint, lower cost and higher yield of target products.

An immersed membrane bioreactor provided by the utility model is characterized in that it contains a biological culture tank 1, an air inlet 2, an air outlet 3, a guide tube 4, a hollow fiber membrane module 5, and a biological culture tank outlet 6 , hollow fiber membrane module port 7, air distributor 8, fermenter culture medium inlet 9; said guide tube 4, air distributor 8 and membrane module 5 are built in biological culture tank 1; said membrane module 5 is hollow The fiber membrane is built in the bottom of the biological culture tank 1 and is located on the upper part of the air distributor 8, with one end open and connected with the external pipeline of the reactor through a gasket.

In the above-mentioned submerged membrane bioreactor, the hollow fiber membrane module 5 is a dynamic annular microfiltration membrane or ultrafiltration membrane module and can withstand a high temperature of 115 ° C. The membrane pore size is 0.01-0.3um. At the lower end inside the device, the port 7 of the hollow fiber membrane module is sealed with the biological culture outlet 6 through a silica gel pad.

In the above-mentioned submerged membrane bioreactor, the guide tube 4 is a frame fixed in the reactor, and the hollow fiber membrane module 5 surrounds the lower end of the guide tube 4; the air distributor 8 is a The annular air distributor is located under the membrane module, and its diameter is the same as that of the annular membrane module 5 .

In the above submerged membrane bioreactor, the membrane bioreactor is an airlift bioreactor, and oxygen is supplied through the annular air distributor 8 at the bottom of the reactor.

The submerged membrane bioreactor of the utility model is composed of a biological cultivation tank, a diversion cylinder, an air distributor and a ring-shaped membrane module; the diversion cylinder, the air distributor and the membrane module are built inside the biological cultivation tank, and the membrane module is hollow The fiber membrane is placed on the upper part of the air distributor, one end is open, connected with the external pipeline of the reactor through the sealing gasket, and the clarified fermentation liquid is obtained by suction by the vacuum pump.

Due to the submerged membrane bioreactor provided by the present invention, the whole reactor and membrane modules are sterilized by high temperature and moist heat, which ensures the pure culture requirements of microorganisms and improves the high value-added secondary growth and metabolism of microorganisms such as Cordyceps sinensis. Generation and separation of metabolites. The utility model can be used in the field of continuous biological pure culture, especially in the field of aerobic cell culture, and has the advantages of small footprint, high-density cell fermentation and high product conversion rate.

Description of drawings

Fig. 1 is a sectional view of a kind of submerged membrane bioreactor applied in the field of pure biological cultivation described in the utility model;

In the figure: 1. Biological culture tank, 2. Air inlet, 3. Air outlet, 4. Guide tube, 5. Hollow fiber membrane module, 6. Biological culture tank outlet, 7. Hollow fiber membrane port, 8. Air Distributor, 9. Fermentation medium inlet.

Detailed ways:

Referring to Fig. 1, a kind of submerged membrane bioreactor applied to the pure culture field of microorganisms described in the utility model comprises a biological culture tank (1), a guide tube (4), an air distributor (8) and an annular Membrane module (5). The hollow fiber membrane module (5) is placed at the bottom of the fermenter and above the air distributor (8). When the instrument is running, when the air bubbles ejected from the air distributor pass through the surface of the hollow fiber membrane during the rising process, the bubbles burst and generate huge Shear force, so as to scour the dynamic membrane, thereby delaying the fouling of the membrane. The port (7) of the hollow fiber membrane and the outlet (6) of the biological culture tank are sealed by a silica gel pad, and are connected to a vacuum pump through a pipeline.

Assemble a membrane bioreactor (5 L), and sterilize the reactor with a sterilization parameter of 115° C. for 30 minutes, and then insert the seed fermentation broth for pure microbial culture. After the fermentation has been carried out for a certain period of time, turn on the peristaltic pump, and continuously add sterile medium into the reactor through the medium inlet (9) of the fermenter; at the same time, turn on the vacuum pump to set a vacuum degree of 0.02MPa, and continuously flow out through the outlet (6) of the fermenter Fermentation broth after membrane treatment. The turbidity of the fermentation broth is 0.49NTU, and the backwash cycle of the hollow fiber membrane module (5) reaches 100h. The fermentation broth flowing out of the membrane bioreactor can be directly used for product purification and other operations without any pretreatment.

Claims (4)

1. A submerged membrane bioreactor, characterized in that: it contains a biological culture tank (1), an air inlet (2), an air outlet (3), a draft tube (4), a hollow fiber membrane module (5 ), biological culture tank outlet (6), hollow fiber membrane module port (7), air distributor (8), fermentor culture medium inlet (9); described guide tube (4), air distributor (8) And the membrane module (5) is built in the inside of the biological culture tank (1); the membrane module (5) is a hollow fiber membrane, which is built in the bottom of the biological culture tank (1), and is located on the top of the air distributor (8), with one end The opening is connected with the external pipeline of the reactor through the gasket. 2. The submerged membrane bioreactor according to claim 1, characterized in that: the hollow fiber membrane module (5) is a dynamic annular microfiltration membrane or ultrafiltration membrane module and can withstand a high temperature of 115 ° C. The pore diameter is 0.01-0.3um, the membrane module is located at the lower end inside the reactor, and the port (7) of the hollow fiber membrane module is sealed with the biological culture outlet (6) through a silica gel pad. 3. The submerged membrane bioreactor according to claim 1, characterized in that: the guide tube (4) is a frame fixed in the reactor, and the hollow fiber membrane module (5) surrounds the guide tube The lower end of the flow tube (4); the air distributor (8) is an annular air distributor located below the membrane module, and its diameter is the same as that of the annular membrane module (5). 4. The submerged membrane bioreactor according to claim 1, characterized in that: the membrane bioreactor is an airlift bioreactor, and oxygen is supplied through the annular air distributor (8) at the bottom of the reactor.