CN208632536U - A kind of microbial cell purifier - Google Patents
A kind of microbial cell purifier Download PDFInfo
- Publication number
- CN208632536U CN208632536U CN201820978262.9U CN201820978262U CN208632536U CN 208632536 U CN208632536 U CN 208632536U CN 201820978262 U CN201820978262 U CN 201820978262U CN 208632536 U CN208632536 U CN 208632536U
- Authority
- CN
- China
- Prior art keywords
- culture bottle
- glass tube
- connector
- microbial cell
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 19
- 239000011521 glass Substances 0.000 claims abstract description 33
- 238000002347 injection Methods 0.000 claims abstract description 10
- 239000007924 injection Substances 0.000 claims abstract description 10
- 230000001105 regulatory effect Effects 0.000 claims description 16
- 244000261422 Lysimachia clethroides Species 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 238000000746 purification Methods 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 12
- 239000000243 solution Substances 0.000 abstract description 11
- 238000002474 experimental method Methods 0.000 abstract description 8
- 238000004140 cleaning Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of microbial cell purifiers, including the first culture bottle and the second culture bottle, inlet tube is communicated at the top of first culture bottle, the top of second culture bottle is filled with gasket, injection port is offered at the top of the gasket, the surface wrap of second culture bottle has coil, the input terminal of the coil is electrically connected with radiofrequency generator, top and bottom on the left of first culture bottle is communicated with the first glass tube, the utility model passes through setting inlet tube, the cooperation of gasket and injection port, achieve the effect that being rapidly injected culture solution in culture bottle, by the way that coil is arranged, radiofrequency generator, first glass tube, second glass tube, connector, the cooperation of snap-gauge and biomembrane, achieve the effect that quickly to filter cell and cell factor, quickly cell is purified to realize Effect brings convenience to the experiment of microbial cell, to improve the efficiency of purification experiment.
Description
Technical field
The utility model relates to microbial cell technical field of purification, specially a kind of microbial cell purifier.
Background technique
In biology, microbiological research and in use, in order to prevent and treat miscellaneous bacteria interference, the microorganism of high-purity is generally required
Cell, conventional microbial cell purifying are all to implement multiple separation repeatedly using selective medium in culture dish, this
The conventional purification process of kind haves the defects that purification time is long, inconvenience is brought to the experiment of microbial cell, to reduce
The efficiency of purification experiment.
Utility model content
The purpose of this utility model is to provide a kind of microbial cell purifier, having can be quickly pure to cell progress
The advantages of change, solving the problems, such as existing purification process, there are purification time length.
To achieve the above object, the utility model provides the following technical solutions: a kind of microbial cell purifier, including the
One culture bottle and the second culture bottle are communicated with inlet tube at the top of first culture bottle, fill out at the top of second culture bottle
Filled with gasket, injection port is offered at the top of the gasket, the surface wrap of second culture bottle has coil, the line
The input terminal of circle is electrically connected with radiofrequency generator, and the top and bottom on the left of first culture bottle is communicated with the first glass tube,
Top and bottom on the right side of second culture bottle is communicated with the second glass tube, first glass tube and the second glass tube phase
Corresponding side is threaded with connector, and the surface of the connector is fixedly connected with snap-gauge, the connection by fixing piece
The surface of part is embedded with biomembrane, and the surface of the biomembrane is fixedly connected with the inner wall of snap-gauge.
Preferably, it is provided with the first regulating valve at the top of the top of first glass tube and the second glass tube, it is described
The side of first culture bottle and the second culture bottle offers outlet tube respectively, and the surface of the outlet tube is provided with the second adjusting
Valve.
Preferably, the surface of the inlet tube is provided with third regulating valve, and the shape of the inlet tube is in gooseneck type.
Preferably, the surface of second glass tube offers the annular groove being used cooperatively with snap-gauge in a ring, and annular groove
Make wearing layer processing in inner cavity.
Preferably, the inner cavity of the connector is hollow structure, and the two sides on the connector surface are provided with screw thread.
Compared with prior art, the beneficial effects of the utility model are as follows:
1, the utility model has been reached by the cooperation of setting inlet tube, gasket and injection port to quick in culture bottle
The effect for injecting culture solution passes through setting coil, radiofrequency generator, the first glass tube, the second glass tube, connector, snap-gauge and biology
The cooperation of film has achieved the effect that quickly to filter cell and cell factor, to realize quickly pure to cell progress
The effect of change solves the problems, such as existing purification process there are purification time length, brings just to the experiment of microbial cell
Benefit, to improve the efficiency of purification experiment.
2, the utility model is adjusted the flow velocity of cell culture fluid by the first regulating valve of setting, passes through setting the
Two regulating valves facilitate the outflow of culture solution, facilitate the operation of experimenter, and the shape by the way that inlet tube is arranged is in gooseneck type, keep away
Bacterial clump is exempted to fall into culture solution, to influence experimental result, by the way that annular groove is arranged, has made the better fit connection of snap-gauge
Rotation, by be arranged annular groove inner cavity make wearing layer processing, reduce the frictional force between annular groove and snap-gauge, to extend
The service life of snap-gauge and annular groove, the two sides by the way that connector surface is arranged are provided with screw thread, have reached and be convenient for connector
The effect of installation and removal, while also facilitating the cleaning of biomembrane.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is partial enlargement diagram at the A of the utility model Fig. 1.
In figure: 1 first culture bottle, 2 second culture bottles, 3 inlet tubes, 4 gaskets, 5 injection ports, 6 coils, 7 radiofrequency generators, 8
First glass tube, 9 second glass tubes, 10 connectors, 11 snap-gauges, 12 biomembranes, 13 first regulating valves, 14 outlet tubes, 15 second
Regulating valve, 16 third regulating valves.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
A kind of microbial cell purifier referring to FIG. 1-2, including the first culture bottle 1 and the second culture bottle 2, the first training
The top for supporting bottle 1 is communicated with inlet tube 3, and the surface of inlet tube 3 is provided with third regulating valve 16, and the shape of inlet tube 3 is in gooseneck
Formula, the shape by the way that inlet tube 3 is arranged is in gooseneck type, avoids bacterial clump and falls into culture solution, so that experimental result is influenced,
The top of second culture bottle 2 is filled with gasket 4, and the top of gasket 4 offers injection port 5, and the surface of the second culture bottle 2 twines
It is wound with coil 6, the input terminal of coil 6 is electrically connected with radiofrequency generator 7, and the top and bottom in 1 left side of the first culture bottle is communicated with
The top and bottom of first glass tube 8,2 right side of the second culture bottle is communicated with the second glass tube 9, the top of the first glass tube 8
The first regulating valve 13 is provided with the top of the second glass tube 9, and the side of the first culture bottle 1 and the second culture bottle 2 is opened respectively
Equipped with outlet tube 14, the surface of outlet tube 14 is provided with the second regulating valve 15, by the way that the first regulating valve 13 is arranged, to cell culture
The flow velocity of liquid is adjusted, and by the second regulating valve 15 of setting, facilitates the outflow of culture solution, facilitates the operation of experimenter, the
The surface of two glass tubes 9 offers the annular groove being used cooperatively with snap-gauge 11 in a ring, and wearing layer processing is made in the inner cavity of annular groove, leads to
Setting annular groove is crossed, the rotation of the better fit connection 10 of snap-gauge 11 is made, wearing layer processing is made in the inner cavity by the way that annular groove is arranged, and subtracts
Lack the frictional force between annular groove and snap-gauge 11, so that the service life of snap-gauge 11 and annular groove is extended, the first glass tube 8 and
The corresponding side of two glass tube 9 is threaded with connector 10, and the inner cavity of connector 10 is hollow structure, 10 surface of connector
Two sides be provided with screw thread, by be arranged 10 surface of connector two sides be provided with screw thread, reached to connector 10 just
In the effect of installation and removal, while the cleaning of biomembrane 12 is also facilitated, the surface of connector 10 connects by the way that fixing piece is fixed
It is connected to snap-gauge 11, the surface of connector 10 is embedded with biomembrane 12, and the surface of biomembrane 12 is fixedly connected with the inner wall of snap-gauge 11,
By the way that the cooperation of inlet tube 3, gasket 4 and injection port 5 is arranged, achieve the effect that being rapidly injected culture solution in culture bottle,
Pass through matching for setting coil 6, radiofrequency generator 7, the first glass tube 8, the second glass tube 9, connector 10, snap-gauge 11 and biomembrane 12
It closes, has achieved the effect that quickly to filter cell and cell factor, to realize the effect for quickly purifying cell
Fruit solves the problems, such as existing purification process there are purification time length, brings convenience to the experiment of microbial cell, thus
Improve the efficiency of purification experiment.
In use, before use, each section of the utility model is carried out cleaning and sterilizing, and connect, to the second training
The culture solution for being added in bottle 2 and required cell being suitble to grow is supported, added culture solution height should be more than superposed second glass tube
9, culture solution is added in the first culture bottle 1, is inoculated with by injection port 5 into the second culture bottle 2, by inlet tube 3 to the first training
It supports and injects cell factor in bottle 1, open the first regulating valve 13, biomembrane 12 allows cell factor to pass through, and cell cannot be made logical
It crosses, different cell factors can be injected into the first culture bottle 1 according to cell growth status, promote the growth of cell to be purified,
Inhibit the growth of other cells, and then achieve the purpose that purifying cells, by the cooperation of coil 6 and radiofrequency generator 7, it is thin to accelerate purifying
The growth of born of the same parents, to realize the effect rapidly purified to cell.
In summary: the microbial cell purifier, by inlet tube 3, gasket 4,5 coil 6 of injection port, radiofrequency generator 7,
The cooperation of first glass tube 8, the second glass tube 9, connector 10, snap-gauge 11 and biomembrane 12, solves existing purification process
There is a problem of purification time length.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of microbial cell purifier, including the first culture bottle (1) and the second culture bottle (2), it is characterised in that: described
It is communicated at the top of one culture bottle (1) inlet tube (3), the top of second culture bottle (2) is filled with gasket (4), described
It is offered at the top of gasket (4) injection port (5), the surface wrap of second culture bottle (2) has coil (6), the coil
(6) input terminal is electrically connected with radiofrequency generator (7), and the top and bottom on the left of first culture bottle (1) is communicated with first
Glass tube (8), the top and bottom on the right side of second culture bottle (2) are communicated with the second glass tube (9), first glass
Pipe (8) side corresponding with the second glass tube (9) is threaded with connector (10), and the surface of the connector (10) passes through
Fixing piece is fixedly connected with snap-gauge (11), and the surface of the connector (10) is embedded with biomembrane (12), the biomembrane (12)
Surface be fixedly connected with the inner wall of snap-gauge (11).
2. a kind of microbial cell purifier according to claim 1, it is characterised in that: first glass tube (8)
It is provided with the first regulating valve (13) at the top of top and the second glass tube (9), first culture bottle (1) and the second culture bottle
(2) side offers outlet tube (14) respectively, and the surface of the outlet tube (14) is provided with the second regulating valve (15).
3. a kind of microbial cell purifier according to claim 1, it is characterised in that: the surface of the inlet tube (3)
It is provided with third regulating valve (16), the shape of the inlet tube (3) is in gooseneck type.
4. a kind of microbial cell purifier according to claim 1, it is characterised in that: second glass tube (9)
Surface offers the annular groove being used cooperatively with snap-gauge (11) in a ring, and wearing layer processing is made in the inner cavity of annular groove.
5. a kind of microbial cell purifier according to claim 1, it is characterised in that: the inner cavity of the connector (10)
Two sides for hollow structure, connector (10) surface are provided with screw thread.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820978262.9U CN208632536U (en) | 2018-06-25 | 2018-06-25 | A kind of microbial cell purifier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820978262.9U CN208632536U (en) | 2018-06-25 | 2018-06-25 | A kind of microbial cell purifier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208632536U true CN208632536U (en) | 2019-03-22 |
Family
ID=65735416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820978262.9U Expired - Fee Related CN208632536U (en) | 2018-06-25 | 2018-06-25 | A kind of microbial cell purifier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208632536U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
-
2018
- 2018-06-25 CN CN201820978262.9U patent/CN208632536U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
| CN113174361B (en) * | 2021-06-10 | 2023-11-14 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190322 |