CN105675864A - Automatic bacteria sorting and marking device based on immune method - Google Patents
Automatic bacteria sorting and marking device based on immune method Download PDFInfo
- Publication number
- CN105675864A CN105675864A CN201610116775.4A CN201610116775A CN105675864A CN 105675864 A CN105675864 A CN 105675864A CN 201610116775 A CN201610116775 A CN 201610116775A CN 105675864 A CN105675864 A CN 105675864A
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- Prior art keywords
- tank
- culture tank
- liquid pump
- conduit
- culture
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000894006 Bacteria Species 0.000 title abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 60
- 239000000758 substrate Substances 0.000 claims abstract description 18
- 239000011324 bead Substances 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 13
- 239000002699 waste material Substances 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 18
- 238000002372 labelling Methods 0.000 claims description 14
- 230000003053 immunization Effects 0.000 claims description 13
- 238000002649 immunization Methods 0.000 claims description 13
- 238000010926 purge Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 9
- 239000002131 composite material Substances 0.000 description 8
- 238000000746 purification Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 2
- 229920001917 Ficoll Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an automatic bacteria sorting and marking device based on an immune method, comprising a substrate; the surface of the substrate is respectively provided with a magnetic bead fluid tank and a fluorescent material tank; the magnetic bead fluid tank is connected with a first liquid pump by a duct; the first liquid pump is connected with a first culture tank by a duct; a first injection hole is formed in the top of the first culture tank; the fluorescent material tank is connected with a second liquid pump by a duct. According to the automatic bacteria sorting and marking device based on the immune method, a controller is used for respectively controlling the amount of culture bacteria injected into a third culture tank by the first culture tank and a second culture tank, so that quantitative and accurate culture is realized; magnetic bead-purpose complex bacteria is absorbed by electromagnet, so that a good effect is achieved; the third culture tank is directly connected with a waste liquid tank and a cleaning pipe, so that cleaning efficiency and purifying efficiency are better improved, the purifying time is short, and the economic benefit is high.
Description
Technical field
The present invention relates to biological technical field, be specially a kind of antibacterial automatic sorting labelling apparatus based on immunization method.
Background technology
Along with the development of life sciences, the Clinics and Practices of biology of bacteria and amynologic basis research and clinical disease is required for from the mixing sample containing different bacterium to separate and purification of bacterial. Existing bacterium separation technology mainly has Ficoll density gradient centrifugation, centrifugal eluting, double water phase separation etc., these methods are all utilize antibacterial size, density, the feature of the physical property such as surface charge is separated, it is difficult to obtain ideal purity and the response rate.
Cell sorting based on magnetic bead is the cell separation technology developed rapidly in recent years, first labelling antibody molecule on magnetic bead, make itself and cell occur specific binding, relend the effect helping external magnetic field, make the cell generation displacement combined with magnetic bead realize separating of purpose cell. The method operation can obtain higher purity. But complex operation in incubation, it is impossible to realizing quantitatively accurately cultivating, purification process is longer, and purification efficiency is relatively low, for this, it is proposed that a kind of antibacterial automatic sorting labelling apparatus based on immunization method.
Summary of the invention
It is an object of the invention to provide a kind of antibacterial automatic sorting labelling apparatus based on immunization method, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme: a kind of antibacterial automatic sorting labelling apparatus based on immunization method, including substrate, the surface of described substrate is respectively equipped with magnetic bead fluid tank and fluorescent material tank, described magnetic bead fluid tank is connected to the first liquid pump by conduit, described first liquid pump connects the first culture tank by conduit, the top of described first culture tank is provided with the first injection port, described fluorescent material tank connects the second liquid pump by conduit, described second liquid pump connects the second culture tank by conduit, the end face of described second culture tank is provided with the second injection port, substrate is all run through in the bottom of described first culture tank and the second culture tank, and first the bottom of culture tank connect the first electromagnetic valve by conduit, described first electromagnetic valve connects the 3rd culture tank by conduit, the bottom of described second culture tank connects the second electromagnetic valve by conduit, described second electromagnetic valve connects the 3rd culture tank by conduit, the top of described 3rd culture tank is provided with electric magnet, the bottom of described 3rd culture tank is provided with outlet, and the 3rd the bottom of culture tank connect the 3rd liquid pump and the 4th liquid pump respectively through conduit, described 3rd liquid pump connects purge tank by conduit, described 4th liquid pump connects waste liquid tank by conduit, described 3rd culture tank, the side, middle part of purge tank and waste liquid tank is all socketed with fixed cover, described fixed cover upper surface is connected by fixing bar is fixing with the bottom surface of substrate, described electric magnet, first liquid pump, second liquid pump, 3rd liquid pump, 4th liquid pump, first electromagnetic valve, second electromagnetic valve all electrically connects with the controller being located at substrate surface.
Preferably, the bottom of described first culture tank and the second culture tank is infundibulate.
Preferably, the catheter interior between described first culture tank and the first electromagnetic valve is provided with first-class gauge, described first-class gauge electrical connection controller.
Preferably, the catheter interior between described second culture tank and the second electromagnetic valve is provided with second gauge, described second gauge electrical connection controller.
Preferably, described 3rd culture tank includes outer tank body and interior tank body, and described interior tank body is located in outer tank body, and leaves space between outer tank body and interior tank body, is provided with spiral heater strip in space, described heater strip electrical connection controller.
Compared with prior art, the invention has the beneficial effects as follows: should based on the antibacterial automatic sorting labelling apparatus of immunization method, the cultivation bacterium amount that the first culture tank and the second culture tank are injected to the 3rd culture tank is controlled respectively by controller, can realize quantitatively accurately cultivating, adsorb magnetic bead-purpose antibacterial complex by electric magnet, effective, 3rd culture tank and waste liquid tank, cleaning pipe are directly connected to, be conducive to improving and clean purification efficiency, purify the used time few, high financial profit.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram;
Fig. 2 is the present invention the 3rd culture tank structural representation.
In figure: 1 substrate, 2 magnetic bead fluid tank, 3 fluorescent material tanks, 4 first culture tank, 5 first injection ports, 6 first liquid pumps, 7 second liquid pumps, 8 second injection ports, 9 second culture tank, 10 first electromagnetic valves, 11 second electromagnetic valves, 12 purge tanks, 13 the 3rd liquid pumps, 14 outlets, 15 the 3rd culture tank, 16 the 4th liquid pumps, 17 fixed covers, 18 waste liquid tanks, 19 fixing bars, 20 heater strips, 151 outer tank body, tank body in 152.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
Refer to Fig. 1-2, the present invention provides a kind of technical scheme: a kind of antibacterial automatic sorting labelling apparatus based on immunization method, including substrate 1, the surface of substrate 1 is respectively equipped with magnetic bead fluid tank 2 and fluorescent material tank 3, magnetic bead fluid tank 2 is connected to the first liquid pump 6 by conduit, first liquid pump 6 connects the first culture tank 4 by conduit, magnetic bead fluid can be injected in the first culture tank 4 by the first liquid pump 6, the top of the first culture tank 4 is provided with the first injection port 5, purpose antibacterial can be injected and carry out the cultivation of magnetic bead composite bacterial, fluorescent material tank 3 connects the second liquid pump 7 by conduit, second liquid pump 7 connects the second culture tank 9 by conduit, fluorescent material can be injected in the second culture tank 9 by the second liquid pump 7, the end face of the second culture tank 9 is provided with the second injection port 8, purpose antibacterial can be injected in the second culture tank 9 and carry out the cultivation of fluorescent material composite bacterial, the bottom of the first culture tank 4 and the second culture tank 9 is infundibulate, conveniently the culture fluid in culture tank is all discharged, substrate 1 is all run through in the bottom of the first culture tank 4 and the second culture tank 9, and first the bottom of culture tank 4 connect the first electromagnetic valve 10 by conduit, catheter interior between first culture tank 4 and the first electromagnetic valve 10 is provided with first-class gauge, can accurate flow control, first-class gauge electrical connection controller, first electromagnetic valve 10 connects the 3rd culture tank 15 by conduit, the bottom of the second culture tank 9 connects the second electromagnetic valve 11 by conduit, catheter interior between second culture tank 9 and the second electromagnetic valve 11 is provided with second gauge, can accurately control the cultivation liquid measure of the discharge of the second culture tank 9, second gauge electrical connection controller, second electromagnetic valve 11 connects the 3rd culture tank 15 by conduit, the top of the 3rd culture tank 15 is provided with electric magnet, the bottom of the 3rd culture tank 15 is provided with outlet 14, and the 3rd culture tank 15 bottom respectively through conduit connect the 3rd liquid pump 13 and the 4th liquid pump 16, 3rd liquid pump 13 connects purge tank 12 by conduit, 4th liquid pump 16 connects waste liquid tank 18 by conduit, 3rd culture tank 15, the side, middle part of purge tank 12 and waste liquid tank 18 is all socketed with fixed cover 17, fixed cover 17 upper surface is connected by fixing bar 19 is fixing with the bottom surface of substrate 1, electric magnet, first liquid pump 6, second liquid pump 7, 3rd liquid pump 13, 4th liquid pump 16, first electromagnetic valve 10, second electromagnetic valve 11 all electrically connects with the controller being located at substrate 1 surface, 3rd culture tank 15 includes outer tank body 151 and interior tank body 152, interior tank body 152 is located in outer tank body 151, and leave space between outer tank body 151 and interior tank body 152, space is provided with spiral heater strip 20, heater strip 20 electrically connects controller, magnetic bead composite bacterial and fluorescent material composite bacterial are cultivated in the 3rd culture tank 15, cultivation temperature is can ensure that by heater strip 20, thus obtaining magnetic bead-fluorescent material composite bacterial, the cleanout fluid in purge tank 12 can be extracted by the 3rd liquid pump 13 and clean cultured composite bacterial, then pass through controller opens electric magnet and composite bacterial is adsorbed on the top of the 3rd culture tank 15, clean the waste liquid formed and enter in waste liquid tank 18 by the 4th liquid pump 16, it is repeatedly performed this process and completes the purification to composite bacterial, should based on the antibacterial automatic sorting labelling apparatus of immunization method, the first culture tank 4 is controlled respectively and cultivation bacterium amount that the second culture tank 9 is injected to the 3rd culture tank 15 by controller, can realize quantitatively accurately cultivating, magnetic bead-purpose antibacterial complex is adsorbed by electric magnet, effective, by the 3rd culture tank 15 and waste liquid tank 18, clean pipe 12 to be directly connected to, be conducive to improving and clean purification efficiency, purify the used time few, high financial profit.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, being appreciated that and these embodiments can be carried out multiple change, amendment, replacement and modification without departing from the principles and spirit of the present invention, the scope of the present invention be defined by the appended.
Claims (5)
1. the antibacterial automatic sorting labelling apparatus based on immunization method, including substrate (1), it is characterized in that: the surface of described substrate (1) is respectively equipped with magnetic bead fluid tank (2) and fluorescent material tank (3), described magnetic bead fluid tank (2) is connected to the first liquid pump (6) by conduit, described first liquid pump (6) connects the first culture tank (4) by conduit, the top of described first culture tank (4) is provided with the first injection port (5), described fluorescent material tank (3) connects the second liquid pump (7) by conduit, described second liquid pump (7) connects the second culture tank (9) by conduit, the end face of described second culture tank (9) is provided with the second injection port (8), substrate (1) is all run through in the bottom of described first culture tank (4) and the second culture tank (9), and first the bottom of culture tank (4) connect the first electromagnetic valve (10) by conduit, described first electromagnetic valve (10) connects the 3rd culture tank (15) by conduit, the bottom of described second culture tank (9) connects the second electromagnetic valve (11) by conduit, described second electromagnetic valve (11) connects the 3rd culture tank (15) by conduit, the top of described 3rd culture tank (15) is provided with electric magnet, the bottom of described 3rd culture tank (15) is provided with outlet (14), and the 3rd the bottom of culture tank (15) connect the 3rd liquid pump (13) and the 4th liquid pump (16) respectively through conduit, described 3rd liquid pump (13) connects purge tank (12) by conduit, described 4th liquid pump (16) connects waste liquid tank (18) by conduit, described 3rd culture tank (15), the side, middle part of purge tank (12) and waste liquid tank (18) is all socketed with fixed cover (17), described fixed cover (17) upper surface is connected by fixing bar (19) is fixing with the bottom surface of substrate (1), described electric magnet, first liquid pump (6), second liquid pump (7), 3rd liquid pump (13), 4th liquid pump (16), first electromagnetic valve (10), second electromagnetic valve (11) all electrically connects with the controller being located at substrate (1) surface.
2. a kind of antibacterial automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that: the bottom of described first culture tank (4) and the second culture tank (9) is infundibulate.
3. a kind of antibacterial automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterized in that: the catheter interior between described first culture tank (4) and the first electromagnetic valve (10) is provided with first-class gauge, described first-class gauge electrical connection controller.
4. a kind of antibacterial automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterized in that: the catheter interior between described second culture tank (9) and the second electromagnetic valve (11) is provided with second gauge, described second gauge electrical connection controller.
5. a kind of antibacterial automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterized in that: described 3rd culture tank (15) includes outer tank body (151) and interior tank body (152), described interior tank body (152) is located in outer tank body (151), and leave space between outer tank body (151) and interior tank body (152), space is provided with spiral heater strip (20), described heater strip (20) electrical connection controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610116775.4A CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610116775.4A CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
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| Publication Number | Publication Date |
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| CN105675864A true CN105675864A (en) | 2016-06-15 |
| CN105675864B CN105675864B (en) | 2017-11-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610116775.4A Expired - Fee Related CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414752A (en) * | 2018-03-28 | 2018-08-17 | 黄河科技学院 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
| WO2021116380A1 (en) | 2019-12-11 | 2021-06-17 | Aixinno Ltd. | Method and device for cultivating biological cells |
| CN113583934A (en) * | 2021-07-30 | 2021-11-02 | 阿谷巴(杭州)生物科技发展有限公司 | Culture medium for rapidly detecting bacteria in cell product and preparation method and application thereof |
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2016
- 2016-02-29 CN CN201610116775.4A patent/CN105675864B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO1999034848A1 (en) * | 1998-01-08 | 1999-07-15 | Nexell Therapeutics Inc. | Method, bag and disposable set for recirculation washing of blood cells |
| CN1263893A (en) * | 1999-02-05 | 2000-08-23 | 上海复旦张江生物医药有限公司 | Renaturation equipment of modified protein |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414752A (en) * | 2018-03-28 | 2018-08-17 | 黄河科技学院 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
| WO2021116380A1 (en) | 2019-12-11 | 2021-06-17 | Aixinno Ltd. | Method and device for cultivating biological cells |
| CN113583934A (en) * | 2021-07-30 | 2021-11-02 | 阿谷巴(杭州)生物科技发展有限公司 | Culture medium for rapidly detecting bacteria in cell product and preparation method and application thereof |
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| CN105675864B (en) | 2017-11-07 |
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Effective date of registration: 20210111 Address after: Room 606, 6th floor, comprehensive building, community entrepreneurship Park, Langya Economic Development Zone, Chuzhou City, Anhui Province Patentee after: Anhui xinjiuya Photoelectric Technology Co.,Ltd. Address before: 252000 No.3, Dongguan Street, Dongchangfu District, Liaocheng City, Shandong Province Patentee before: Dong Yujun |
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