CN220724198U - Biological cell culture device - Google Patents
Biological cell culture device Download PDFInfo
- Publication number
- CN220724198U CN220724198U CN202321620140.XU CN202321620140U CN220724198U CN 220724198 U CN220724198 U CN 220724198U CN 202321620140 U CN202321620140 U CN 202321620140U CN 220724198 U CN220724198 U CN 220724198U
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- overflow
- pipe
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- cell culture
- valve
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- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000007599 discharging Methods 0.000 claims description 10
- 230000007246 mechanism Effects 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 8
- 239000000428 dust Substances 0.000 claims description 5
- 239000007789 gas Substances 0.000 abstract description 22
- 238000002474 experimental method Methods 0.000 abstract description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 108010066114 cabin-2 Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 239000002775 capsule Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a biological cell culture device, and relates to the technical field of biological cell culture. The utility model comprises a supporting plate and an air cabin, wherein the air cabin is fixedly arranged at the top of the supporting plate, one end of the air cabin is communicated with an air inlet pipe, and a flowmeter is arranged on the air inlet pipe; the overflow groove is fixedly arranged at the top of the air cabin, overflow pipes are communicated above two sides of the overflow groove at equal intervals, and a first valve is arranged at the position of the overflow pipe body, which is close to the overflow groove; the gas delivery pipes are communicated with the overflow pipe body at equal intervals, and one end, far away from the overflow pipe, of each gas delivery pipe is communicated with the gas cabin. The utility model realizes the respective culture of cells in liquid environments with different oxygen concentrations and components by utilizing the arrangement of the overflow pipe and the gas transmission pipe and the control of the first valve and the second valve, and can simultaneously perform a plurality of groups of cell culture experiments by additionally arranging a plurality of culture cylinders, thereby improving the working efficiency.
Description
Technical Field
The utility model relates to the technical field of biological cell culture equipment, in particular to a biological cell culture device.
Background
Cell culture refers to a method for simulating in vitro in vivo environment (asepsis, proper temperature, pH value, certain nutrition condition and the like) to enable the cells to survive, grow, reproduce and maintain main structures and functions, and a large number of cells can be obtained through cell culture, and signal transduction of the cells, anabolism of the cells, growth and proliferation of the cells and the like can be studied by the cell culture.
When biological cells are cultured, the cells are often required to be placed in different culture solutions with different components or in environments with different oxygen concentrations for respective culture, and in the prior art, workers often need to perform experiments for a plurality of times, which greatly prolongs the experimental period and has lower working efficiency, so that a biological cell culture device is provided for solving the problems.
Disclosure of Invention
The utility model adopts the following technical scheme for realizing the purposes:
a biological cell culture device, comprising:
the device comprises a supporting plate and an air cabin, wherein the air cabin is fixedly arranged at the top of the supporting plate, one end of the air cabin is communicated with an air inlet pipe, and a flowmeter is arranged on the air inlet pipe;
the overflow groove is fixedly arranged at the top of the air cabin, overflow pipes are communicated above two sides of the overflow groove at equal intervals, and a first valve is arranged at the position of the overflow pipe body, which is close to the overflow groove;
the gas transmission pipes are communicated with the overflow pipe body at equal intervals, one end of each gas transmission pipe far away from the overflow pipe is communicated with the gas cabin, and a second valve is arranged at the position, close to the gas cabin, of each gas transmission pipe;
the culture cylinder is communicated with one end of the overflow pipe far away from the overflow groove, and one side of the top of the culture cylinder is communicated with an exhaust pipe;
and the waste liquid collecting mechanism is used for discharging the redundant culture liquid in the overflow groove.
Further, the first valve and the second valve are both check valves.
Further, the gas pipe is obliquely arranged, and one end close to the gas cabin is higher than one end close to the overflow pipe.
Further, one end of the overflow pipe far away from the overflow groove is provided with a connector, and the connector is in threaded connection with the upper port of the culture cylinder.
Further, the exhaust pipe is arranged obliquely upwards, and one end of the exhaust pipe, which is far away from the culture cylinder, is provided with a dust screen.
Further, the waste liquid collecting mechanism comprises a discharging pipe communicated with the bottom side of one end of the overflow chute, a third valve is arranged at the position, close to the overflow chute, of the pipe body of the discharging pipe, a positioning groove is formed in the position, corresponding to the lower port of the discharging pipe, of the supporting plate, and a collecting cup is placed in the positioning groove.
The beneficial effects of the utility model are as follows:
according to the utility model, by means of the arrangement of the overflow pipe and the gas pipe and the control of the first valve and the second valve, the respective culture of cells in liquid environments with different oxygen concentrations and components is realized by a controlled variable method, and a plurality of groups of cell culture experiments can be simultaneously carried out by additionally installing a plurality of culture cylinders, so that the working efficiency is improved, the experiment period is greatly shortened, the time cost of workers is reduced, the device is simple and convenient to operate, the experiment efficiency and accuracy are improved, and the device has high significance and practicability for biomedical research and application and is worthy of general application and popularization.
Drawings
FIG. 1 is a schematic perspective view of the present utility model;
FIG. 2 is a schematic view of another perspective structure of the present utility model;
FIG. 3 is a schematic view of the structure of the air delivery pipe of the present utility model.
Reference numerals: 1. a supporting plate; 2. an air chamber; 3. an air inlet pipe; 4. a flow meter; 5. an overflow trough; 6. an overflow pipe; 601. a connector; 7. a first valve; 8. a gas pipe; 9. a second valve; 10. a culture drum; 11. an exhaust pipe; 12. a waste liquid collection mechanism; 1201. a discharge pipe; 1202. a third valve; 1203. a collection cup.
Description of the embodiments
In order to make the objects, technical solutions and advantages of the embodiments of the present utility model more clear, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.
The application provides a biological cell culture device, and mainly used solves the problem that staff often need to carry out experiments for many times in the prior art, which greatly prolongs the experimental period and has lower working efficiency, and provides the following technical scheme, and the following detailed description will be made with reference to fig. 1-3:
a biological cell culture device, comprising:
the device comprises a supporting plate 1 and an air cabin 2, wherein the air cabin 2 is fixedly arranged at the top of the supporting plate 1, one end of the air cabin 2 is communicated with an air inlet pipe 3, and a flowmeter 4 is arranged on the air inlet pipe 3;
the overflow groove 5 is fixedly arranged at the top of the air chamber 2, overflow pipes 6 are communicated above two sides of the overflow groove 5 at equal intervals, and a first valve 7 is arranged at the position of the pipe body of the overflow pipe 6 close to the overflow groove 5;
the gas delivery pipes 8 are communicated with the pipe body of the overflow pipe 6 at equal intervals, one end of each gas delivery pipe 8, which is far away from the overflow pipe 6, is communicated with the gas cabin 2, and a second valve 9 is arranged at the position, which is close to the gas cabin 2, of each gas delivery pipe 8;
a culture cylinder 10 communicated with one end of the overflow pipe 6 far away from the overflow groove 5, and an exhaust pipe 11 is communicated with one side of the top of the culture cylinder 10;
and a waste liquid collecting mechanism 12 for discharging the excess culture liquid in the overflow tank 5.
When the biological cell culture device is used, different culture solution components can be added into the overflow tank 5 for a plurality of times (the culture solution has several components for several times), for example, when the influence of the component A on cell culture is required to be studied, when the component A is added, the first valve 7 on the overflow pipe 6 corresponding to the reference group culture cylinder 10 is closed, so that the culture solution enters the culture cylinder 10 corresponding to the experiment group, as the culture solution flows into the culture cylinder 10 from the overflow pipe 6 in an overflow manner, the consistency of the components of the experiment group and other culture solutions of the reference group can be ensured, and after one culture solution component is added, the redundant culture solution of the overflow tank 5 is required to be discharged through the waste solution collecting mechanism 12, so that the influence experiment of the different culture solution components on cell culture is realized, and in addition, the total flow rate of oxygen can be calculated by observing the inside the air cabin 2 through the air inlet pipe 3, and the culture result of the cells under the environment of different oxygen concentration can be studied by controlling the quantity of closing and opening the second valve 9.
As shown in fig. 3, in some embodiments, the first valve 7 and the second valve 9 are both one-way valves, more specifically, the first valve 7 is a one-way valve such that fluid can only flow from the overflow tank 5 to the culture vessel 10 and not from the culture vessel 10 to the overflow tank 5, and the second valve 9 is a one-way valve such that gas can flow from the gas capsule 2 to the overflow pipe 6 and not from the overflow pipe 6 to the gas capsule 2.
In some embodiments, as shown in fig. 3, the air pipe 8 is disposed obliquely, and the end near the air chamber 2 is higher than the end near the overflow pipe 6, and more specifically, the air pipe 8 is disposed obliquely so as to avoid the culture solution from entering the air chamber 2.
As shown in fig. 3, in some embodiments, a connector 601 is provided at one end of the overflow pipe 6 away from the overflow groove 5, the connector 601 is in threaded connection with the upper port of the culture cylinder 10, more specifically, the connector 601 is in threaded connection with the culture cylinder 10, so that the culture cylinder 10 can be conveniently detached and replaced, the operation is very simple, and meanwhile, the fitting degree and stability of the pipeline are ensured.
As shown in fig. 3, in some embodiments, the exhaust pipe 11 is disposed obliquely upward, and a dust screen is disposed at an end of the exhaust pipe away from the culture cylinder 10, more specifically, the exhaust pipe 11 is generally configured to exhaust the exhaust gas in the culture cylinder 10, so as to keep the culture environment relatively stable, and the exhaust pipe 11 is configured obliquely upward to cooperate with the dust screen, so that bacteria, dust, other particles and other substances can be prevented from entering the culture cylinder 10 through the exhaust pipe 11, the culture environment is polluted, and experimental equipment is damaged.
As shown in fig. 2, in some embodiments, the waste liquid collecting mechanism 12 includes a discharging pipe 1201 communicated with the bottom side of one end of the overflow tank 5, a third valve 1202 is installed at the position of the pipe body of the discharging pipe 1201 close to the overflow tank 5, a positioning groove is provided at the position of the pallet 1 corresponding to the lower port of the discharging pipe 1201, and a collecting cup 1203 is placed inside the positioning groove, more specifically, when the third valve 1202 is opened, liquid is discharged along the discharging pipe 1201 and finally enters the collecting cup 1203, the function of the positioning groove is to stabilize the collecting cup 1203, prevent the situation that the collecting cup 1203 moves and inclines in the experimental process, ensure the accuracy and reliability of experimental data, and meanwhile, the placing of the collecting cup 1203 is relatively convenient, and can be replaced and cleaned easily, thereby improving the experimental efficiency.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A biological cell culture device, comprising:
the device comprises a supporting plate (1) and an air cabin (2), wherein the air cabin (2) is fixedly arranged at the top of the supporting plate (1), one end of the air cabin (2) is communicated with an air inlet pipe (3), and a flowmeter (4) is arranged on the air inlet pipe (3);
the overflow groove (5) is fixedly arranged at the top of the air cabin (2), overflow pipes (6) are communicated above two sides of the overflow groove (5) at equal intervals, and a first valve (7) is arranged at the position, close to the overflow groove (5), of the pipe body of the overflow pipe (6);
the gas delivery pipes (8) are communicated with the pipe body of the overflow pipe (6) at equal intervals, one end, far away from the overflow pipe (6), of each gas delivery pipe (8) is communicated with the gas cabin (2), and a second valve (9) is arranged at the position, close to the gas cabin (2), of each gas delivery pipe (8);
a culture cylinder (10) communicated with one end of the overflow pipe (6) far away from the overflow groove (5), wherein one side of the top of the culture cylinder (10) is communicated with an exhaust pipe (11);
and a waste liquid collection mechanism (12) for discharging the excess culture liquid from the overflow tank (5).
2. A biological cell culture device according to claim 1, wherein the first valve (7) and the second valve (9) are both one-way valves.
3. A biological cell culture device according to claim 1, wherein the gas delivery tube (8) is arranged obliquely and the end near the gas chamber (2) is higher than the end near the overflow tube (6).
4. A biological cell culture device according to claim 1, characterized in that the overflow pipe (6) is provided with a connector (601) at the end far away from the overflow groove (5), and the connector (601) is in threaded connection with the upper port of the culture cylinder (10).
5. A biological cell culture device according to claim 1, wherein the exhaust pipe (11) is arranged obliquely upwards and the end thereof remote from the culture cylinder (10) is provided with a dust screen.
6. The biological cell culture device according to claim 1, wherein the waste liquid collecting mechanism (12) comprises a discharge pipe (1201) communicated with the bottom side of one end of the overflow tank (5), a third valve (1202) is arranged at the position, close to the overflow tank (5), of the pipe body of the discharge pipe (1201), a positioning groove is formed in the position, corresponding to the lower port of the discharge pipe (1201), of the supporting plate (1), and a collecting cup (1203) is arranged in the positioning groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321620140.XU CN220724198U (en) | 2023-06-26 | 2023-06-26 | Biological cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321620140.XU CN220724198U (en) | 2023-06-26 | 2023-06-26 | Biological cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220724198U true CN220724198U (en) | 2024-04-05 |
Family
ID=90488564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321620140.XU Active CN220724198U (en) | 2023-06-26 | 2023-06-26 | Biological cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220724198U (en) |
-
2023
- 2023-06-26 CN CN202321620140.XU patent/CN220724198U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240429 Address after: 1 / F, building 28, 6055 Jinhai highway, Fengxian District, Shanghai 201400 Patentee after: Shanghai Aizhi Biotechnology Co.,Ltd. Country or region after: China Address before: Room 308 and 310, 3rd Floor, Building 7, No. 128 Wanshui Road, Ninghu Community, Changning Street, High tech Zone, Hefei City, Anhui Province, 230000 (Provincial Academy of Sciences Health Incubator) Patentee before: Anhui Hefeng Biotechnology Co.,Ltd. Country or region before: China |