CN205333360U - Plant is cultivateed and root exudates collection device - Google Patents

Plant is cultivateed and root exudates collection device Download PDF

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Publication number
CN205333360U
CN205333360U CN201620117267.3U CN201620117267U CN205333360U CN 205333360 U CN205333360 U CN 205333360U CN 201620117267 U CN201620117267 U CN 201620117267U CN 205333360 U CN205333360 U CN 205333360U
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China
Prior art keywords
bucket
conveying pipe
valve
plant
separating column
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Expired - Fee Related
Application number
CN201620117267.3U
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Chinese (zh)
Inventor
马军
黄绪堂
张树权
吴广文
王文军
郭永利
袁红梅
张利国
王静
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Institute Of Industrial Crops Of Heilongjiang Academy Of Agricultural Sciences
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Institute Of Industrial Crops Of Heilongjiang Academy Of Agricultural Sciences
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Priority to CN201620117267.3U priority Critical patent/CN205333360U/en
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Publication of CN205333360U publication Critical patent/CN205333360U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a plant is cultivateed and root exudates collection device, the utility model relates to a plant culture apparatus. The problem of current can not once only collect plant roots secretion in a large number, can not satisfy from now on the needs of further developing and using plant roots secretion, the device uses the operation complicated, and the practicality is not strong is solved. The device: the collecting pipe upper end is passed cultivation barrel head portion and is set up in cultivateing the bucket, and the collecting pipe lower extreme communicates with the inlet of resin separator column, the inner chamber of cultivation bucket from top to bottom be equipped with and train matrix and glass cotton, the income gas end of oxygen therapy pump even has a second conveyer pipe, pumps third conveyer pipe that the gas end is connected with oxygen therapy and passes during nutrient solution tank deck portion rubber buffer inserts the nutrition fluid reservoir, the fourth conveyer pipe that is connected with peristaltic pump income water end (W. E. ) passes nutrient solution tank deck portion rubber buffer and inserts in the nutrition fluid reservoir, it opens wide during the mouth stretches into the inner chamber of training the bucket through training the bucket upper end to pump first conveyer pipe that the water end (W. E. ) is connected with the wriggling.

Description

A kind of plant culturing and root exudates collection device
Technical field
This utility model relates to plant cultivation device。
Background technology
Root exudates refers to the general name of all kinds of materials that root system is discharged in surrounding。Allelopathy between the activation of mineral nutrient, alleviation and elimination heavy metallic poison and plant is played very important effect by the low-molecular-weight organic matters such as the organic acid of root system secretion and aldehydes matter。When certain plants is subject to nutrient stress, the release of low molecular weight organic acid can be promoted, make Soil Phosphorus, ferrum, zinc, manganese biological effectiveness be greatly improved;Low molecular weight organic acid also can activate heavy metal element such as plumbous and cadmium, and people may utilize the heavy metal pollution in this effect environment purification。
In the research of current plant science, secretions from plant roots has become as the object of study of many scholars, but its relatively low content and difficult quality meet extensive high-throughout research requirement, this makes to become the important technical bottleneck limiting the development of this field by the conventional culture methods obtained for the purpose of root exudates。Up to the present, existing method also exists obvious shortcoming and defect, it is impossible to disposable a large amount of collection secretions from plant roots, it is impossible to meet the needs from now on secretions from plant roots developed further, and device uses complicated operation, and practicality is not strong。
Utility model content
The purpose of this utility model is to provide a kind of plant culturing and root exudates collection device。With solve existing can not disposable a large amount of collection secretions from plant roots, it is impossible to meeting the needs from now on secretions from plant roots developed further, device uses complicated operation, the problem that practicality is not strong。
A kind of plant culturing and root exudates collection device include support, cultivate bucket, culture matrix, glass cotton, first fixing bar, second fixing bar, illumination cultivation light, collecting pipe, first valve, first drainage screen, resin separating column, thermal insulation cover, returnable bottle, oxygen therapy pump, nutrient tank, peristaltic pump, second valve, 3rd valve, microbe filter film, 4th valve, first conveying pipe, second drainage screen, humiture detector, second conveying pipe, 3rd conveying pipe, 4th conveying pipe and monitoring device;
Collecting pipe upper end is arranged in cultivation bucket through cultivating bottom of the barrel, collecting pipe lower end connects with the inlet of resin separating column, the liquid outlet of resin separating column arranges the second drainage screen, and the liquid outlet of resin separating column is arranged on the top of returnable bottle, cultivating bucket and be fixed on support by the first fixing bar, resin separating column is fixed on support by the second fixing bar;Illumination cultivation light is arranged on above cultivation bucket and is fixed on support;The described inner chamber cultivating bucket is from top to bottom provided with culture matrix and glass cotton;Described collecting pipe is from top to bottom sequentially provided with the first valve and the first drainage screen;The periphery of described resin separating column is provided with thermal insulation cover;
The air inlet end of oxygen therapy pump is connected with the second conveying pipe, the second described conveying pipe is provided with the second valve, the 3rd conveying pipe being connected with oxygen therapy pump outlet side inserts in nutrient tank through nutrient tank top rubber plug, and the 3rd described conveying pipe is sequentially provided with the 3rd valve and microbe filter film;
The 4th conveying pipe being connected with peristaltic pump water intake end inserts in nutrient tank through nutrient tank top rubber plug, and the 4th described conveying pipe is provided with the 4th valve;
In the inner chamber that the first conveying pipe being connected with peristaltic pump water side cultivates bucket by cultivating bucket open upper end mouth to stretch into;
It is provided with humiture detector in described culture matrix, and humiture detector is connected with monitoring device by wire。
The beneficial effects of the utility model are: 1, this utility model is after plant growing a period of time, require that the original position that can carry out root exudates at any time is collected according to experiment purpose, do not destroy the upgrowth situation of root system of plant, the root exudates of different biology plant in period can be collected, and the situation of plant root exudation under natural conditions can be reacted。
2, this utility model supply sufficient nutrition of plant and fully oxygen, according to growth and development of plants need configuration nutritional solution in nutrient tank, inject to cultivate in bucket by peristaltic pump by nutritional solution and meet growth and development of plants needs, simultaneously by curing by oxygen therapy pump and illumination cultivation light to meet growth and development of plants needs。
3, the thermal insulation cover filling ice bag is installed in resin separating column periphery, the root exudates stable in properties at low temperatures being adsorbed on resin can be ensured, prevent degraded and the composition transfer of root exudates in collection process, extend the collection cycle of root exudates, add the collecting amount of root exudates。
4, the first drainage screen can avoid the loss of culture matrix, is conducive to the collection of root exudates。
5, this utility model uses peristaltic pump, it is not necessary to worry, because the deficiency of current, to cause water pump to evacuate and break down。This utility model can pass through to change the flow velocity of peristaltic pump, and then adds the response rate of root exudates。
6, humiture detector carries out the record of plant root growth data, and is sent in external monitoring device (such as computer etc.) to be calculated these record data and show。Researcher is conducive to utilize this device to carry out the scientific research of root growth, growth and the aspect such as alimentation and utilization。
7, this utility model assembles simple, and processing ease can obtain the free of contamination root exudates of high-quality, without any other operation bidirectional in whole incubation, it is ensured that the high cleanliness of secretions from plant roots, it is ensured that the accuracy of subsequent experimental。
This utility model is for a kind of plant culturing and root exudates collection device。
Accompanying drawing explanation
Fig. 1 is this utility model one plant culturing and root exudates collection device。
Detailed description of the invention
Technical solutions of the utility model are not limited to the detailed description of the invention of act set forth below, also include the combination in any between each detailed description of the invention。
Detailed description of the invention one: illustrate present embodiment below in conjunction with Fig. 1。A kind of plant culturing and the root exudates collection device of present embodiment include support 1, cultivate bucket 2, culture matrix 3, glass cotton 4, first fixing bar 5, second fixing bar 6, illumination cultivation light 7, collecting pipe 8, first valve 9, first drainage screen 10, resin separating column 11, thermal insulation cover 12, returnable bottle 13, oxygen therapy pump 14, nutrient tank 15, peristaltic pump 16, second valve 17, 3rd valve 18, microbe filter film 19, 4th valve 20, first conveying pipe 21, second drainage screen 22, humiture detector 24, second conveying pipe 26, 3rd conveying pipe 27, 4th conveying pipe 28 and monitoring device 29;
Collecting pipe 8 upper end is arranged in cultivation bucket 2 bottom cultivation bucket 2, collecting pipe 8 lower end connects with the inlet of resin separating column 11, the liquid outlet of resin separating column 11 arranges the second drainage screen 22, and the liquid outlet of resin separating column 11 is arranged on the top of returnable bottle 13, cultivating bucket 2 and be fixed on support 1 by the first fixing bar 5, resin separating column 11 is fixed on support 1 by the second fixing bar 6;Illumination cultivation light 7 is arranged on above cultivation bucket 2 and is fixed on support 1;The described inner chamber cultivating bucket 2 is from top to bottom provided with culture matrix 3 and glass cotton 4;Described collecting pipe 8 is from top to bottom sequentially provided with the first valve 9 and the first drainage screen 10;The periphery of described resin separating column 11 is provided with thermal insulation cover 12;
The air inlet end of oxygen therapy pump 14 is connected with the second conveying pipe 26, the second described conveying pipe 26 is provided with the second valve 17, the 3rd conveying pipe 27 being connected with oxygen therapy pump 14 outlet side inserts in nutrient tank 15 through nutrient tank 15 top rubber plug, and the 3rd described conveying pipe 27 is sequentially provided with the 3rd valve 18 and microbe filter film 19;
The 4th conveying pipe 28 being connected with peristaltic pump 16 water intake end inserts in nutrient tank 15 through nutrient tank 15 top rubber plug, and the 4th described conveying pipe 28 is provided with the 4th valve 20;
In the inner chamber that the first conveying pipe 21 being connected with peristaltic pump 16 water side cultivates bucket 2 by cultivating bucket 2 open upper end mouth to stretch into;
It is provided with humiture detector 24 in described culture matrix 3, and humiture detector 24 is connected with monitoring device 29 by wire。
Detailed description of the invention provides the benefit that: 1, this detailed description of the invention is after plant growing a period of time, require that the original position that can carry out root exudates at any time is collected according to experiment purpose, do not destroy the upgrowth situation of root system of plant, the root exudates of different biology plant in period can be collected, and the situation of plant root exudation under natural conditions can be reacted。
2, the supply sufficient nutrition of plant of this detailed description of the invention and fully oxygen, according to growth and development of plants need configuration nutritional solution in nutrient tank 15, nutritional solution is injected in cultivation bucket 2 by peristaltic pump 16 and meets growth and development of plants needs, simultaneously by curing by oxygen therapy pump 14 and illumination cultivation light 7 to meet growth and development of plants needs。
3, at the peripheral thermal insulation cover 12 installing filling ice bag of resin separating column 11, the root exudates stable in properties at low temperatures being adsorbed on resin can be ensured, prevent degraded and the composition transfer of root exudates in collection process, extend the collection cycle of root exudates, add the collecting amount of root exudates。
4, the first drainage screen 10 can avoid the loss of culture matrix 3, is conducive to the collection of root exudates。
5, this detailed description of the invention uses peristaltic pump 16, it is not necessary to worry, because the deficiency of current, to cause water pump to evacuate and break down。This utility model can pass through to change the flow velocity of peristaltic pump, and then adds the response rate of root exudates。
6, humiture detector 24 carries out the record of plant root growth data, and is sent in external monitoring device 29 (such as computer etc.) to be calculated these record data and show。Researcher is conducive to utilize this device to carry out the scientific research of root growth, growth and the aspect such as alimentation and utilization。
7, this detailed description of the invention assembles simple, and processing ease can obtain the free of contamination root exudates of high-quality, without any other operation bidirectional in whole incubation, it is ensured that the high cleanliness of secretions from plant roots, it is ensured that the accuracy of subsequent experimental。
Detailed description of the invention two: present embodiment and detailed description of the invention one the difference is that: described illumination cultivation light 7 is provided with illumination cultivation light timer 25。Other is identical with detailed description of the invention one。
Detailed description of the invention three: one of present embodiment and detailed description of the invention one or two the difference is that: the aperture of the second described drainage screen 22 is less than the particle diameter of resin in resin separating column 11。Other is identical with detailed description of the invention one。
Operation principle: first take off resin separating column 11, the first valve 9 being arranged on collecting pipe 8 is closed, cultivate the culture matrix 3 after loading onto process in bucket 2, to need the plant species collected in culture matrix 3, add nutritional solution saturated to culture matrix 3, after plant strain growth a period of time, start to collect root exudates, first deionized water is poured in cultivation bucket 2, deionized water is utilized to clean culture matrix 3, open the first valve 9, solution after cleaning is bled off, then resin separating column 11 is connected on collection collecting pipe 8, at the peripheral thermal insulation cover 12 installing filling ice bag of resin separating column 11, control nutritional solution flow, collect root exudates。The each parts of device, culture matrix 3, glass cotton 4, adsorbent resin, nutritional solution and plant seedlings all carry out cleaning before use, sterilization treatment。

Claims (3)

1. a plant culturing and root exudates collection device, it is characterized in that a kind of plant culturing and root exudates collection device include support (1), cultivate bucket (2), culture matrix (3), glass cotton (4), first fixing bar (5), second fixing bar (6), illumination cultivation light (7), collecting pipe (8), first valve (9), first drainage screen (10), resin separating column (11), thermal insulation cover (12), returnable bottle (13), oxygen therapy pump (14), nutrient tank (15), peristaltic pump (16), second valve (17), 3rd valve (18), microbe filter film (19), 4th valve (20), first conveying pipe (21), second drainage screen (22), humiture detector (24), second conveying pipe (26), 3rd conveying pipe (27), 4th conveying pipe (28) and monitoring device (29);
Collecting pipe (8) upper end is arranged in cultivation bucket (2) through cultivating bucket (2) bottom, collecting pipe (8) lower end connects with the inlet of resin separating column (11), the liquid outlet of resin separating column (11) arranges the second drainage screen (22), and the liquid outlet of resin separating column (11) is arranged on the top of returnable bottle (13), cultivating bucket (2) and be fixed on support (1) by the first fixing bar (5), resin separating column (11) is fixed on support (1) by the second fixing bar (6);Illumination cultivation light (7) is arranged on cultivation bucket (2) top and is fixed on support (1);The inner chamber of described cultivation bucket (2) is from top to bottom provided with culture matrix (3) and glass cotton (4);Described collecting pipe (8) is from top to bottom sequentially provided with the first valve (9) and the first drainage screen (10);The periphery of described resin separating column (11) is provided with thermal insulation cover (12);
The air inlet end of oxygen therapy pump (14) is connected with the second conveying pipe (26), the second described conveying pipe (26) is provided with the second valve (17), the 3rd conveying pipe (27) being connected with oxygen therapy pump (14) outlet side is inserted in nutrient tank (15) through nutrient tank (15) top rubber plug, and the 3rd described conveying pipe (27) is sequentially provided with the 3rd valve (18) and microbe filter film (19);
The 4th conveying pipe (28) being connected with peristaltic pump (16) water intake end is inserted in nutrient tank (15) through nutrient tank (15) top rubber plug, and the 4th described conveying pipe (28) is provided with the 4th valve (20);
In the inner chamber that the first conveying pipe (21) being connected with peristaltic pump (16) water side cultivates bucket (2) by cultivating bucket (2) open upper end mouth to stretch into;
It is provided with humiture detector (24) in described culture matrix (3), and humiture detector (24) is connected with monitoring device (29) by wire。
2. a kind of plant culturing according to claim 1 and root exudates collection device, it is characterised in that described illumination cultivation light (7) is provided with illumination cultivation light intervalometer (25)。
3. a kind of plant culturing according to claim 1 and root exudates collection device, it is characterised in that the aperture of described the second drainage screen (22) is less than the particle diameter of resin separating column (11) interior resin。
CN201620117267.3U 2016-02-05 2016-02-05 Plant is cultivateed and root exudates collection device Expired - Fee Related CN205333360U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620117267.3U CN205333360U (en) 2016-02-05 2016-02-05 Plant is cultivateed and root exudates collection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620117267.3U CN205333360U (en) 2016-02-05 2016-02-05 Plant is cultivateed and root exudates collection device

Publications (1)

Publication Number Publication Date
CN205333360U true CN205333360U (en) 2016-06-22

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CN201620117267.3U Expired - Fee Related CN205333360U (en) 2016-02-05 2016-02-05 Plant is cultivateed and root exudates collection device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717212A (en) * 2019-01-28 2019-05-07 广西大学 A kind of method that disease-resistant stock root exudates is collected and extracted

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717212A (en) * 2019-01-28 2019-05-07 广西大学 A kind of method that disease-resistant stock root exudates is collected and extracted

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160622

Termination date: 20220205

CF01 Termination of patent right due to non-payment of annual fee