CN204630769U - Plant individual plant aseptic culture root exudates gathering-device - Google Patents
Plant individual plant aseptic culture root exudates gathering-device Download PDFInfo
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- CN204630769U CN204630769U CN201520343493.9U CN201520343493U CN204630769U CN 204630769 U CN204630769 U CN 204630769U CN 201520343493 U CN201520343493 U CN 201520343493U CN 204630769 U CN204630769 U CN 204630769U
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Abstract
One Plants individual plant aseptic culture root exudates gathering-device, comprises plant strain growth bag, root growth pipe, liquid discharging tube and secretion collector that order is from top to bottom connected; Root growth Guan Youyi block net-like spacer is separated into upper and lower two Room, and upper room is root system supported chamber, and indoor hold aseptic sandstone or perlite; Lower room is nutrient solution supply chamber, indoor splendid attire plant nutrition liquid, and this nutrient solution can be full of whole root growth pipe by net-like spacer as required.The utility model design is ingenious, structure is simple, cell layout is flexible, can be effectively used to the collection of root exudates, and aseptic and inclusion-free impact can be accomplished, and the repetition in test can be set, therefore can be widely used in root exudates and collect test.
Description
Technical field
The utility model relates to agricultural engineering technology, particularly relates to a Plants individual plant aseptic culture root exudates gathering-device.
Background technology
Root exudates to refer in plant normal growth process a series of chemical substances discharged in outwardly environment, as proton, low molecular organic acids, amino acid, phenolic acid, soluble protein, soluble sugar, cell detachment thing etc.It is the important carrier material that Plants and Soils carries out mass exchange and information transmission.About the collection method of root exudates, existing a large amount of research worker reports, main conventional method is the collection utilizing nutritional liquid grown plant thus carry out root system nutrition liquid, this method can carry out collecting continuously fast to root exudates, there is certain practicality, but plant root growth resistance when root system is under water planting condition is little and aeration is poor, differ larger with the truth of root exudates under soil cultivation condition.Other utilizes substrate culture plant to regather the method for root exudates, but have no idea to realize the gnotobasis that plant is cultivated, root exudates is easy to be decomposed by the microorganisms and utilizes and produce new organism, therefore the authenticity of the secretions from plant roots collected can not be ensured, and device is complicated, complex operation.In general, the collection method of secretions from plant roots is also immature, and a lot of aspect needs research further.
Utility model content
The purpose of this utility model, exactly in order to solve the problem, provides a kind of novel plant individual plant aseptic culture root exudates gathering-device.
In order to achieve the above object, the utility model have employed following technical scheme: a Plants individual plant aseptic culture root exudates gathering-device, comprises plant strain growth bag, root growth pipe, liquid discharging tube and secretion collector that order is from top to bottom connected; Root growth Guan Youyi block net-like spacer is separated into upper and lower two Room, and upper room is root system supported chamber, and indoor hold aseptic sandstone or perlite; Lower room is nutrient solution supply chamber, indoor splendid attire plant nutrition liquid, and this nutrient solution can be full of whole root growth pipe by net-like spacer as required.
Described plant strain growth bag is clear hard polybag, and bag is provided with multiple bleeder vent.
Described root growth pipe is transparent toughened glass pipe.
Described root growth pipe is cylindrical tube, and the growth type of its caliber and plant is suitable.
The root system supported chamber of described root growth pipe and nutrient solution supply chamber are marked respectively and are provided with scale.
Described liquid discharging tube is detachably connected with secretion collector, and liquid discharging tube is provided with tapping piston.
Described plant strain growth bag is connected on root growth pipe by draw-string, and unclamping draw-string can take off plant strain growth bag, is convenient to filling or replacing nutrient solution in root growth pipe.
The utility model design is ingenious, structure is simple, cell layout is flexible, can be effectively used to the collection of root exudates, and aseptic and inclusion-free impact can be accomplished, and the repetition in test can be set, therefore can be widely used in root exudates and collect test.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model.
Embodiment
See Fig. 1, plant individual plant aseptic culture root exudates gathering-device of the present utility model, comprises plant strain growth bag 1, root growth pipe 2, liquid discharging tube 3 and secretion collector 4 that order is from top to bottom connected; Root growth pipe 2 is separated into upper and lower two Room by one piece of net-like spacer 21, and upper room is root system supported chamber 22, and indoor hold aseptic sandstone or perlite; Lower room is nutrient solution supply chamber 23, indoor splendid attire plant nutrition liquid, and this nutrient solution can be full of whole root growth pipe by net-like spacer as required.
Plant strain growth bag 1 in the utility model is clear hard polybag, bag is provided with multiple bleeder vent 11, plant strain growth bag is connected on root growth pipe 2 by draw-string 12, and unclamping draw-string can take off plant strain growth bag, is convenient to filling or replacing nutrient solution in root growth pipe.
Root growth pipe in the utility model is transparent toughened glass pipe, and switch is cylindrical tube, and the growth type of its caliber and plant is suitable.Its root system supported chamber and nutrient solution supply chamber are marked respectively and are provided with scale.
Liquid discharging tube 3 in the utility model is detachably connected with secretion collector 4, and liquid discharging tube is provided with tapping piston 31.
During concrete use, all parts of device all do sterilization treatment.First will be in the little transplantation of seedlings of the plant germinateed in gnotobasis in root growth pipe 2, then the nutrient solution of sterilizing is injected the nutrient solution supply chamber 23 of root growth pipe, nutrition liquid measure is determined by root system of plant length always.Plant strain growth bag 1 to cover in after injecting on growth tube 2 and utilizes draw-string 12 to fix by nutrient solution, and plant has sufficient clean room to grow.Whole device is placed in clean illumination box cultivate.In superclean bench, change nutrient solution weekly and collect root exudates.Open piston 31, nutrient solution flows in secretion collector 4 together with liquid discharging tube 3 with root exudates.For ensureing that the thorough available aseptic ultrapure water of collection of root exudates repeatedly cleans and collects.
Claims (7)
1. a Plants individual plant aseptic culture root exudates gathering-device, is characterized in that: comprise plant strain growth bag, root growth pipe, liquid discharging tube and secretion collector that order is from top to bottom connected; Root growth Guan Youyi block net-like spacer is separated into upper and lower two Room, and upper room is root system supported chamber, and indoor hold aseptic sandstone or perlite; Lower room is nutrient solution supply chamber, indoor splendid attire plant nutrition liquid, and this nutrient solution can be full of whole root growth pipe by net-like spacer as required.
2. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, is characterized in that: described plant strain growth bag is clear hard polybag, and bag is provided with multiple bleeder vent.
3. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, is characterized in that: described root growth pipe is transparent toughened glass pipe.
4. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, is characterized in that: described root growth pipe is cylindrical tube, and the growth type of its caliber and plant is suitable.
5. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, is characterized in that: the root system supported chamber of described root growth pipe and nutrient solution supply chamber are marked respectively and be provided with scale.
6. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, it is characterized in that: described liquid discharging tube is detachably connected with secretion collector, liquid discharging tube is provided with tapping piston.
7. plant individual plant aseptic culture root exudates gathering-device as claimed in claim 1, it is characterized in that: described plant strain growth bag is connected on root growth pipe by draw-string, unclamp draw-string plant strain growth bag to be taken off, be convenient to filling or replacing nutrient solution in root growth pipe.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201520343493.9U CN204630769U (en) | 2015-05-25 | 2015-05-25 | Plant individual plant aseptic culture root exudates gathering-device |
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CN201520343493.9U CN204630769U (en) | 2015-05-25 | 2015-05-25 | Plant individual plant aseptic culture root exudates gathering-device |
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CN204630769U true CN204630769U (en) | 2015-09-09 |
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CN201520343493.9U Expired - Fee Related CN204630769U (en) | 2015-05-25 | 2015-05-25 | Plant individual plant aseptic culture root exudates gathering-device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
-
2015
- 2015-05-25 CN CN201520343493.9U patent/CN204630769U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
CN109406244B (en) * | 2018-12-29 | 2023-10-10 | 中国科学院南京地理与湖泊研究所 | Aquatic plant root system secretion normal position collection device |
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Date | Code | Title | Description |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150909 Termination date: 20160525 |
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CF01 | Termination of patent right due to non-payment of annual fee |