CN103411793B - Apparatus for collecting root secretions through aerosol culture process, and collecting method thereof - Google Patents

Apparatus for collecting root secretions through aerosol culture process, and collecting method thereof Download PDF

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Publication number
CN103411793B
CN103411793B CN201310301381.2A CN201310301381A CN103411793B CN 103411793 B CN103411793 B CN 103411793B CN 201310301381 A CN201310301381 A CN 201310301381A CN 103411793 B CN103411793 B CN 103411793B
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China
Prior art keywords
root
collecting
liquid storage
nutrient solution
storage bottle
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Expired - Fee Related
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CN201310301381.2A
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Chinese (zh)
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CN103411793A (en
Inventor
张爱华
雷锋杰
张连学
许永华
赵岩
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Jilin Agricultural University
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Jilin Agricultural University
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Abstract

The invention relates to an apparatus for collecting root secretions through an aerosol culture process, and a collecting method thereof, and belongs to the technical field of the agriculture engineering. The apparatus for collecting the root secretions through the aerosol culture process is formed by a culture box, a planting bowl, a nutritional solution box, a collecting bottle, a liquid storage bottle and a resin column, the planting bowl is installed on the top cover of the culture box, the nutritional solution box is provided with an ultrasonic atomizer and a supply pump, the collecting bottle is fixed on the net plate at the top of the nutritional solution box, the bottleneck of the collecting bottle is provided with a collecting funnel, the collecting bottle is connected with the liquid storage bottle, and the liquid storage bottle is connected with the resin column. The collecting method comprises the following steps: selecting plant seedlings, planting in the planting bowl, carrying out atomization injection of a nutritional liquid on plant roots, and culturing until harvesting. The detection data of the plant root secretions in the invention are accurate and precise, and the method is especially suitable for scientific research workers to research the ecology of the plant roots, the root nutrients and the relevant cultivation technology of the roots through utilizing the apparatus.

Description

A kind of aerosol cultivation collects device and the collection method thereof of root exudates
Technical field
The invention belongs to agricultural engineering technology field, is a kind of device collecting secretions from plant roots, and utilizes this device to collect the method for secretions from plant roots.
Background technology
Existing root exudates collection method is by hydroponics, and directly collect root system washing lotion, the time of collection is short., there is the secretions from plant roots jamming pattern challenge once can not collecting secretions from plant roots and collection in a large number in excretion collecting device for root system of plant complicated operation, and workload is large, and error is large, precision is low.
Summary of the invention
The technical problem to be solved in the present invention is to provide the device that a kind of aerosol cultivation collects root exudates.
Invention also provides the method utilizing this device to collect secretions from plant roots.
The scheme of technical solution problem of the present invention adopts incubator, culture pan, nutrient solution case, receiving flask, liquid storage bottle and resin column form the device that aerosol cultivation collects root exudates, culture pan is arranged on the top cover of incubator, the first half of culture pan is outside at incubator, the latter half is inner at incubator, the bottom of nutrient solution case in incubator, nutrient solution case is provided with ultrasonic ultrasonic delay line memory and supports pump, nutrient solution box top is leaking net plate, receiving flask is fixed on leaking net plate, the bottleneck of receiving flask is inserted with collection bucket, collection bucket is immediately below culture pan, liquid storage bottle is outside at incubator, receiving flask is connected by drainage tube, liquid storage bottle connects resin column by bend pipe, bend pipe is provided with valve.
In order to prevent being mixed into impurity, liquid storage bottle is airtight container, and liquid storage bottle bottleneck has gas outlet.
Bottom resin column, there is waste liquid returnable bottle, the waste liquid by resin column can be reclaimed, preventing pollution.
Utilize the method for this device collection secretions from plant roots as follows;
1. adopt above-mentioned aerosol cultivation to collect the device of root exudates, choose without the excellent plant shoots of scab growing way, the rhizome portion meche bag of seedling is bound in rear implantation culture pan, root extend into the inside of incubator through culture pan;
2. the nutrient solution 1.7MHz-2.4MHz ultrasound wave in nutrient solution case is carried out atomization process, form ultrasound wave nutrient solution aerosol, be ejected on root system of plant, wherein nutrient solution liquid level is higher than ultrasonic ultrasonic delay line memory 3-5cm, carry out cultivating until gather, the pump of oxygen supply is simultaneously to nutrient solution oxygen supply;
3., when root system of plant liquid manure reaches capacity state, unnecessary moisture gets off oozy for root system material elutes to flow into receiving flask, then enters to liquid storage bottle by drainage tube;
4. when being full of root exudates liquid in liquid storage bottle, open the valve on bend pipe, the root exudates collected flows into resin column by this bend pipe lower end and carries out enrichment.
Apply the aerial fog cultivation that patent of the present invention has carried out industrial crops tomato and safflower tobacco, and traditional soil cultivation method compares.
Adopt uniform design, test ultrasound wave aerosol cultivates the impact of tomato on Tomato Root System and root exudates, and wherein control group is soil cultivation tomato.
Cultivated days Root fresh weight is than contrast increase/% Root system accelerates/ Secretion is than contrast increase/% Purity/%
7 days 4.48 8 5.26 38.25
15 days 9.32 12 7.82 42.34
30 days 15.61 16 13.95 45.83
Adopt uniform design, test ultrasound wave aerosol cultivates the impact of biennial safflower tobacco plant on safflower tobacco root system and root exudates, and wherein control group is soil cultivation safflower tobacco.
Cultivated days Root fresh weight is than contrast increase/% Root system accelerates/ Secretion is than contrast increase/% Purity/%
10 days 6.36 13 8.44 40.31
20 days 14.75 25 15.39 43.30
30 days 22.62 33 24.68 48.64
The present invention well solves the equilibrium problem of growing process Zhong Shui, fertilizer, gas three.Due to root growth in atmosphere, eliminate other cultivation matrixes such as soil to the resistance of root growth, make root growth more vigorous, fibrous root quantity obviously increases, and root growth speed is fast and biological yield significantly improves; Simultaneously because plant root growth is in airtight growth case, blocked the route of transmission of the phytopathy original, so the oozy secretion of root system is reduced greatly by the chance that Institute of Micro-biology decomposes, purity improves more than 35% than conventional soil cultivation purity.Artificial less to the interference of plant roots in collection process, the data in the secretions from plant roots thus detected are more accurate, and precision is higher.Being particularly suitable for researcher utilizes this device to study the ecological and rhizosphere nutrient of plant rhizosphere and relevant cultivation technique problem thereof.
Accompanying drawing explanation
Accompanying drawing 1 is the cut-open view of apparatus of the present invention.
Embodiment
Apparatus of the present invention are by incubator 1, culture pan 2, nutrient solution case 3, receiving flask 7, liquid storage bottle 10 and resin column 14 are formed, incubator 1 inside surface is black, culture pan 2 is arranged on the top cover of incubator 1, the first half of culture pan 2 is outside at incubator 1, the latter half is inner at incubator 1, the bottom of nutrient solution case 3 in incubator 1, nutrient solution case 3 is provided with ultrasonic ultrasonic delay line memory 4 and supports pump 5, nutrient solution case 3 top is leaking net plate 6, receiving flask 7 is fixed on leaking net plate 6, the bottleneck of receiving flask 7 is inserted with collection bucket 8, collection bucket 8 is immediately below culture pan 2, liquid storage bottle 10 is outside at incubator 1, receiving flask 7 is connected by drainage tube 9, liquid storage bottle 10 connects resin column 14 by bend pipe 12, bend pipe 12 is provided with valve 13, liquid storage bottle 10 is airtight container, liquid storage bottle 10 bottleneck has gas outlet 11, waste liquid returnable bottle 15 is had bottom resin column 14.
The method of collecting secretions from plant roots adopts said apparatus, chooses without the excellent plant shoots of scab growing way, the rhizome portion meche bag of seedling is bound in rear implantation culture pan 2, and root extend into the inside of incubator 1 through culture pan 2; Nutrient solution 1.7MHz-2.4MHz ultrasound wave in nutrient solution case 3 is carried out atomization process, form ultrasound wave nutrient solution aerosol, be ejected on root system of plant, wherein nutrient solution liquid level is higher than ultrasonic ultrasonic delay line memory 3-5cm, carry out cultivating until gather, the pump 5 of oxygen supply is simultaneously to nutrient solution oxygen supply; When root system of plant liquid manure reaches capacity state, unnecessary moisture gets off to flow into receiving flask 7 oozy for root system material elutes, then enters to liquid storage bottle 10 by drainage tube 9; When being full of root exudates liquid in liquid storage bottle 10, open the valve 13 on bend pipe 12, the root exudates collected flows into resin column 14 by this bend pipe 12 lower end and carries out enrichment.

Claims (4)

1. the device of an aerosol cultivation collection root exudates, it is characterized in that: it is primarily of incubator, culture pan, nutrient solution case, receiving flask, liquid storage bottle and resin column are formed, culture pan is arranged on the top cover of incubator, the first half of culture pan is outside at incubator, the latter half is inner at incubator, the bottom of nutrient solution case in incubator, nutrient solution case is provided with ultrasonic ultrasonic delay line memory and supports pump, nutrient solution box top is leaking net plate, receiving flask is fixed on leaking net plate, the bottleneck of receiving flask is inserted with collection bucket, collection bucket is immediately below culture pan, liquid storage bottle is outside at incubator, receiving flask is connected by drainage tube, liquid storage bottle connects resin column by bend pipe, bend pipe is provided with valve.
2. aerosol cultivation according to claim 1 collects the device of root exudates, it is characterized in that: liquid storage bottle is airtight container, and liquid storage bottle bottleneck has gas outlet.
3. aerosol cultivation according to claim 1 collects the device of root exudates, it is characterized in that: have waste liquid returnable bottle bottom resin column.
4. the device of aerosol cultivation collection root exudates according to claim 1 carries out the method for collecting secretions from plant roots, it is characterized in that being completed by following steps:
(1) adopt above-mentioned aerosol cultivation to collect the device of root exudates, choose without the excellent plant shoots of scab growing way, the rhizome portion meche bag of seedling is bound in rear implantation culture pan, root extend into the inside of incubator through culture pan;
(2) nutrient solution 1.7MHz-2.4MHz ultrasound wave is carried out atomization process, form ultrasound wave nutrient solution aerosol, be ejected on root system of plant, carry out cultivating until gather, wherein nutrient solution liquid level is higher than ultrasonic ultrasonic delay line memory 3-5cm, supports pump to nutrient solution oxygen supply simultaneously;
(3) when root system of plant liquid manure reaches capacity state, unnecessary moisture gets off oozy for root system material elutes to flow into receiving flask, then enters to liquid storage bottle by drainage tube;
(4) when being full of root exudates liquid in liquid storage bottle, open the valve on bend pipe, the root exudates collected flows into resin column by this bend pipe lower end and carries out enrichment.
CN201310301381.2A 2013-07-18 2013-07-18 Apparatus for collecting root secretions through aerosol culture process, and collecting method thereof Expired - Fee Related CN103411793B (en)

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Publication number Priority date Publication date Assignee Title
CN103759978A (en) * 2014-02-10 2014-04-30 金陵科技学院 Multi-combined device for sterile collection of plant root exudates and application thereof
CN104236947A (en) * 2014-09-10 2014-12-24 云南农业大学 Collection device for root exudates and using method of collection device
CN107976330B (en) * 2017-11-21 2019-10-18 东北师范大学 The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil
CN109169239A (en) * 2018-09-13 2019-01-11 郑州源冉生物技术有限公司 A kind of water plant culture apparatus

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