CN102845367A - Automation running-water type cultivation device of daphnia magna - Google Patents
Automation running-water type cultivation device of daphnia magna Download PDFInfo
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- CN102845367A CN102845367A CN2012103635549A CN201210363554A CN102845367A CN 102845367 A CN102845367 A CN 102845367A CN 2012103635549 A CN2012103635549 A CN 2012103635549A CN 201210363554 A CN201210363554 A CN 201210363554A CN 102845367 A CN102845367 A CN 102845367A
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Abstract
The invention relates to an automation running-water type cultivation device of daphnia magna. The device comprises six components including a feed culturing room, a cultivation ground substance storage room, a female daphnia feeding room, a young daphnia feeding room, a return room and a power system, wherein water outlet tubes at bottoms of the feed culturing room and the cultivation ground substance storage room are connected with the female daphnia feeding room through the power system; a water outlet tube at the bottom of the female daphnia feeding room is connected with the top of the young daphnia feeding room; a water outlet tube on the top of the young daphnia feeding room is connected with the top of the return room; and a water outlet tube at the bottom of the return room is connected with the cultivation ground substance storage room.
Description
Technical field
The invention belongs to plankton cultivation field, be specifically related to a kind of Daphnia magna automation water-flowing type aquaculture device.
Background technology
Daphnia magna (Daphnia magna Straus) belongs to Arthropoda, Crustachia, cladocera, is common crustacean plankton, is indispensable constituent in the fresh water food chain.Short because of its life cycle, growth is fast, high, the easy cultivation of reproduction amount, water environment is coerced the characteristics such as responsive make it become the widely used model organism in domestic and international laboratory, has important effect aspect the test of the acute toxicity and chronic toxicity of agricultural chemicals, chemicals and environmental hormone effects.
A plurality of international organizations such as present European OECD (OECD), European Union, the U.S., Japan, Korea S and China and country have all formed systematized Daphnia magna toxicity testing standard method.The method is all made with length of time of Daphnia magna, culture medium, condition of culture etc. test and being explicitly called for.Yet the at present Daphnia magna in the most of laboratories of China cultivation lacks effective equipment or device, only can rely on the manual operation of machinery, and not only efficient is low in this manual operation, workload is large, and is difficult to obtain the test young Magna in the required specific length of time.This is for bringing certain difficulty and larger error with Daphnia magna as the multiple testing of biological subject.
Summary of the invention
The objective of the invention is to overcome the defective of existing artificial Daphnia magna cultural technique, a kind of Daphnia magna automation water-flowing type aquaculture device is provided.
This Daphnia magna automatic breeding device comprises feed culturing room, cultivation matrix locker room, female Magna receptacle, young Magna receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, and feed culturing room one side is installed growth lamp, and feed culturing room links to each other with female Magna receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female Magna receptacle and young Magna receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female Magna receptacle and the junction of cone, flowing water Valve is positioned on the filter screen, children Magna receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female Magna receptacle links to each other with young Magna receptacle, and the pipe end that stretches into an end of young Magna receptacle connects a glass vertical tube, described glass vertical tube links to each other with atmosphere, children Magna receptacle links to each other with return passage by a water outlet straight tube near the top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube that links to each other with young Magna receptacle, and return passage bottom outlet pipe links to each other with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are installed between feed culturing room and the female Magna receptacle, and another peristaltic pump and siphonic system are installed between cultivation matrix locker room and the female Magna receptacle.
Described feed culturing room is for the culturing room of cultivating green alga;
Described female Magna receptacle and young Magna receptacle are column container transparent, the inertia material;
Described female Magna receptacle connects a glass vertical tube with the pipe end (stretching into an end of young Magna receptacle) that young Magna receptacle links, and this vertical tube top links to each other with atmosphere.
Wherein, described return passage is the column container of transparent, the inertia material of bottom taper.
Wherein, described top, cultivation matrix locker room and return passage are with high.
Operation logic of the present invention is as follows:
Disposable inoculation green alga and keep suitable condition (22～25 ℃ of temperature, illumination 3000lux, bottom aeration) to cultivate after 5～7 days in the A of feed culturing room can form the Daphnia magna algae liquid of feeding.In the Daphnia magna cultivation matrix B of locker room, female Magna receptacle C, young Magna receptacle D and return passage E, pour respectively an amount of medium into, until liquid level reaches alpha levels.
Adopt timer 2,4 to control respectively peristaltic pump 1,3, the peristaltic pump flow can be regulated as required voluntarily.
In female Magna receptacle C, access healthy, great-hearted Daphnia magna (density is 100～200 every liter), start whole device.This moment, peristaltic pump 1 timing entered female Magna receptacle C with a small amount of algae liquid pump, and peristaltic pump 3 regularly pumps into female Magna receptacle C with the fresh cultivation medium of part.And according to siphon principle, the reduction of B liquid level must cause the medium back flow among the return passage E, and the D medium flows among the E, and the C medium flows among the D, so just forms the dynamic circulation system of B-C-D-E-B.In the water circulation process, the young Magna that female Magna produces just can constantly enter from C among the young Magna receptacle D with current by the filter screen of female Magna receptacle C bottom, realizes that young Magna regularly collects; Algae liquid can be brought among C, the D with current, realizes feeding at regular time and quantity to female Magna and young Magna; Fresh medium also can constantly enter among C, the D with current, realizes dynamically updating of culture medium.In the flow process of current from D to E, the existence of delivery port filter screen prevents that on the one hand young Magna from flowing out, and filters out on the other hand particle, guarantees the cleaning of recirculation water.According to operator's demand, select the suitable time can from D, collect the young Magna in the suitable length of time.
The present invention has following beneficial effect:
1, can realize the dynamic cultivation green alga;
2, the Daphnia magna of feeding at regular time and quantity to automation greatly reduces manpower, and scale of feeding more precisely, feeding time is more reasonable;
3, realize the Daphnia magna cultivating system the circulation of MEDIA FLOW ability of swimming, upgrade, avoid the Daphnia magna anoxic and suppressed or dead, reduce numerous and diverse work of artificial changing water;
4, optionally collect the young Magna in the different length of times, for the Daphnia magna toxotest provides applicable tested Magna, save a large amount of manpowers;
5, round Realization automation of the present invention, cost is low, is easy to realize commercially producing and promoting.
Description of drawings:
Fig. 1: the structural representation of Daphnia magna automatic breeding device of the present invention.
Embodiment:
Below in conjunction with accompanying drawing the present invention is described in further details.
This specific embodiment only is explanation of the invention; it is not limitation of the present invention; those skilled in the art can make the modification that does not have creative contribution to the present embodiment as required after reading this specification, but as long as all are subject to the protection of Patent Law in claim scope of the present invention.
Embodiment 1: Daphnia magna automatic breeding device as shown in Figure 1, and wherein each symbol or letter representation are as follows: A-feed culturing room; B-cultivation matrix locker room; The female Magna receptacle of C-; D-children Magna receptacle; The E-return passage; 1,3 is peristaltic pump; 2,4 be timer; The 5-valve; The 6-35 mesh filter screen; The 7-80 mesh filter screen; The 8-growth lamp; α, β-waterline.
This title and size of installing each main member is as follows:
The A of feed culturing room: diameter 20cm, height 100cm, capacity is 25L
Cultivation matrix apotheca B: diameter 20cm, height 100cm, capacity is 25L
Female Magna receptacle C: diameter 20cm, height 40cm, capacity is 10L
Children Magna receptacle D: diameter 20cm, height 40cm, capacity is 10L
Return passage E: diameter 10cm, height 40cm, capacity is 3.0L
Peristaltic pump 1 and 2:5 (ml/min)
Valve 5: copper
Filter screen 6: stainless steel, mesh 35 orders;
Filter screen 7: nylon material, mesh 80 orders;
Vertical tube 8: diameter 1cm, high 30cm;
Right angle overflow pipe 9: diameter 0.5cm;
All band growth lamp 10: power 60w.
The A of feed culturing room links to each other with female Magna receptacle C by peristaltic pump respectively with cultivation matrix apotheca B; Female Magna receptacle C links to each other with young Magna receptacle D by the bottom outlet pipe, and tube connector gos deep into vertical tube 8 of the vertical connection of an end of young Magna receptacle, and the vertical tube top connects air; Children Magna receptacle links to each other with return passage E by a water outlet straight tube, 3 layer of 80 purpose nylon wire of water outlet straight tube mouth place parcel; Return passage bottom outlet pipe links to each other with cultivation matrix apotheca B, locates, than the continuous lower slightly 1cm At The Height of straight tube (being β-waterline place) of young Magna receptacle right angle overflow pipe 9 is set near the return passage top.
The method of operating of Daphnia magna automatic breeding device of the present invention comprises the steps:
1) algae liquid feed is cultivated
1. the A of closure feed culturing room flowing water Valve adds the medium of 20L in the A of feed culturing room, then adds the chlorella vulgaris (or other algae) that is in right amount increased logarithmic phase, makes its initial frustule concentration reach 10
4～10
5Individual/mL.
2. open the A of feed culturing room bottom aeration valve continuous micro aeration, open growth lamp.
3. frustule concentration rises to 10 after 5 days
5～10
6Individual/mL
4. add medium, each 3000mL 2 times among every circumferentially A of feed culturing room.
2) female Magna inoculation and raising
1. the medium that adds respectively aeration 24h in female Magna receptacle C, the young Magna receptacle D is until liquid level all reaches alpha levels; The medium that adds aeration 24h in matrix apotheca B and the return passage E is until liquid level all reaches the β level.Because liquid level (alpha levels) exceeds B, E liquid level (β level) 1cm among C, the D, therefore under siphonage among C, the D liquid flow among the E successively, in case liquid level is higher than the β level among the E, liquid namely overflows by right angle overflow pipe 9.
2. open matrix apotheca B bottom aeration valve and continue aeration.
3. in female Magna receptacle C, add 200～250 health, great-hearted Daphnia magna.
4. timer 2 and 4 is set as respectively and per hour starts 5min, open peristaltic pump, under time set, peristaltic pump per hour moves 5 minutes, carries respectively medium and the algae liquid feed of oxygen enrichment to female Magna receptacle and young Magna receptacle.
When 3. peristaltic pump starts among the B liquid level be lower than the β level, under siphonage, liquid backflow enters among the B until B, E liquid level maintain an equal level among the E.
4. Daphnia magna produces the little Magna of the first-born after 8 days, and when peristaltic pump starts, under current drive, young Magna will enter young Magna receptacle by the filter screen of female Magna receptacle bottom, and algae liquid also is admitted to young Magna receptacle simultaneously.The existence of vertical tube 8 has then stoped young Magna oppositely to fall to swim among female Magna receptacle C.
3) young Magna collects
1. new born young Magna per hour compiles once to young Magna receptacle, gets Magna n in age hour young Magna according to the research needs, can be after n hour, and the valve of opening young Magna receptacle side carries out young Magna and collects.
A kind of Daphnia magna automatic breeding device of the present invention is described by concrete example, those skilled in the art can use for reference content of the present invention, the links such as appropriate change raw material, process conditions realize corresponding other purpose, its relevant change does not all break away from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and all be deemed to be included within the scope of the present invention.
Claims (6)
1. Daphnia magna automation water-flowing type aquaculture device is characterized in that: described cultivator comprises feed culturing room, cultivation matrix locker room, female Magna receptacle, young Magna receptacle, return passage and six parts of dynamical system; The bottom of described feed culturing room and cultivation matrix apotheca sets out water pipe, flowing water Valve and aeration valve, and feed culturing room one side is installed growth lamp, and feed culturing room links to each other with female Magna receptacle by peristaltic pump respectively with the outlet pipe of cultivation matrix apotheca; Female Magna receptacle and young Magna receptacle are the column container of bottom taper, there are filter screen and flowing water Valve in the cylinder of female Magna receptacle and the junction of cone, flowing water Valve is positioned on the filter screen, children Magna receptacle bottom sets out water pipe and flowing water Valve, the bottom outlet pipe of female Magna receptacle links to each other with young Magna receptacle, children Magna receptacle links to each other with return passage by a water outlet straight tube near the top, water outlet straight tube mouth parcel filter screen; Return passage arranges a right angle overflow pipe near a mouthful place, top, and its height is a little less than the straight tube that links to each other with young Magna receptacle, and return passage bottom outlet pipe links to each other with cultivation matrix apotheca; Described dynamical system, comprise two peristaltic pumps and siphonic system, two peristaltic pumps have respectively separately independently Timer Controlling, peristaltic pump and siphonic system are installed between feed culturing room and the female Magna receptacle, and another peristaltic pump and siphonic system are installed between cultivation matrix locker room and the female Magna receptacle.
2. according to claim 1 cultivator, described feed culturing room is for the culturing room of cultivating green alga.
3. according to claim 1 cultivator, described female Magna receptacle and young Magna receptacle are column container transparent, the inertia material.
4. according to claim 1 cultivator, described female Magna receptacle is connected a glass vertical tube with the pipe end of young Magna receptacle one end that link to each other and that stretch into young Magna receptacle, and described glass vertical tube links to each other with atmosphere.
5. according to claim 1 cultivator, described return passage are the column container of transparent, the inertia material of bottom taper.
6. according to claim 1 cultivator, described top, cultivation matrix locker room and return passage are with high.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012103635549A CN102845367B (en) | 2012-09-26 | 2012-09-26 | Automation running-water type cultivation device of daphnia magna |
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|---|---|---|---|
| CN2012103635549A CN102845367B (en) | 2012-09-26 | 2012-09-26 | Automation running-water type cultivation device of daphnia magna |
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| CN102845367A true CN102845367A (en) | 2013-01-02 |
| CN102845367B CN102845367B (en) | 2013-12-04 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563859A (en) * | 2013-11-18 | 2014-02-12 | 华中农业大学 | Device and method for replacing nutrient solution for indoor cultivation of daphnia magna |
| CN103598150A (en) * | 2013-11-30 | 2014-02-26 | 哈尔滨工业大学 | Rapid cultivation device and method of daphnia magna laboratory |
| CN104026081A (en) * | 2014-06-11 | 2014-09-10 | 温州中普知识产权有限公司 | Raising device for crustacean plankton |
| CN106442959A (en) * | 2016-10-10 | 2017-02-22 | 环境保护部南京环境科学研究所 | Running water type toxicity test device for daphnia magna |
| CN107318748A (en) * | 2017-05-14 | 2017-11-07 | 上海海洋大学 | A kind of Biological control device for aquarium |
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Patent Citations (7)
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| JPH09172902A (en) * | 1995-12-27 | 1997-07-08 | Marino Forum 21 | Continuous culture method for animal plankton |
| WO2002003786A1 (en) * | 2000-07-06 | 2002-01-17 | Nagase & Co., Ltd. | Method of culturing zooplankton, apparatus for culturing by the same, and culture obtained by the same |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563859A (en) * | 2013-11-18 | 2014-02-12 | 华中农业大学 | Device and method for replacing nutrient solution for indoor cultivation of daphnia magna |
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| CN103598150B (en) * | 2013-11-30 | 2015-12-02 | 哈尔滨工业大学 | Daphnia magna laboratory fast culture device and cultural method |
| CN104026081A (en) * | 2014-06-11 | 2014-09-10 | 温州中普知识产权有限公司 | Raising device for crustacean plankton |
| CN106442959A (en) * | 2016-10-10 | 2017-02-22 | 环境保护部南京环境科学研究所 | Running water type toxicity test device for daphnia magna |
| CN106442959B (en) * | 2016-10-10 | 2018-09-07 | 环境保护部南京环境科学研究所 | Daphnia magna continuous-flow type toxicity test device |
| CN107318748A (en) * | 2017-05-14 | 2017-11-07 | 上海海洋大学 | A kind of Biological control device for aquarium |
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| Publication number | Publication date |
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| CN102845367B (en) | 2013-12-04 |
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