CN103563859A

CN103563859A - Device and method for replacing nutrient solution for indoor cultivation of daphnia magna

Info

Publication number: CN103563859A
Application number: CN201310580712.0A
Authority: CN
Inventors: 王永模; 刘慧�; 李广胜
Original assignee: Huazhong Agricultural University
Current assignee: Huazhong Agricultural University
Priority date: 2013-11-18
Filing date: 2013-11-18
Publication date: 2014-02-12

Abstract

The invention discloses a device and method for replacing a nutrient solution for indoor cultivation of daphnia magna and belongs to the technical field of nutrient solution replacement of water creatures. The device comprises a water trough (10), a baffle (20), a new nutrient solution (30), an old nutrient solution (40) and a bulb (50). The water trough (10) is divided into a left water trough (11) and a right water trough (12) by the baffle (20) arranged in the middle of the water trough (10). The middle of the bottom of the baffle (20) is provided with a small hole (21) and a rubber plug (22) of the small hole (21). The new nutrient solution (30) is contained in the left water trough (11), the old nutrient solution (40) and the daphnia magna (00) are poured into the right water trough (12), and the bulb (50) is arranged at the left side of the water trough (10). According to the device and method for exchanging the nutrient solution for indoor cultivation of the daphnia magna, possible physical injury to a daphnia magna body is avoided in the process of separation; food debris, excreta of the daphnia magna, slough and the like in the old nutrient solution are basically prevented from entering the new nutrient solution; compared with a normal suction method, the method has the advantages that the separation efficiency is improved and the separation time is shortened.

Description

Devices and methods therefor for indoor cultivation Daphnia magna replacing culture fluid

Technical field

The present invention relates to aquatic organism and change the technical field of culture fluid, relate in particular to a kind of devices and methods therefor for indoor cultivation Daphnia magna replacing culture fluid.

Background technology

Daphnia magna also claims water flea or scratches, and is commonly called as red worm or worm for fishing, belongs to cladocera (Cladocera), refers to the animal of Arthropoda, Crustachia, Branchiopoda, two Leptostraca, branch angle suborder.Daphnia magna has great scientific research value as a kind of model organism of environmental pollution monitoring.

When utilizing Daphnia magna to carry out scientific experimentation, often need to Daphnia magna, manually cultivate indoor, due to what often take, it is static culture, therefore need often Daphnia magna to be carried out to the replacing of culture fluid, to remove casting off a skin and some swills of Daphnia magna generation, for fear of not changing for a long time culture fluid, do not make its PH concentration that larger change occur simultaneously yet.

The mode of traditional replacing culture fluid is carefully Daphnia magna individuality to be transferred in turn in new culture fluid and gone with pipette, and this not only expends time in, and often by some residues and cast off a skin to transfer in the lump in new culture fluid and go.

Summary of the invention

Object of the present invention is just to overcome the shortcoming and defect that prior art exists, and a kind of devices and methods therefor for indoor cultivation Daphnia magna replacing culture fluid is provided.

The object of the present invention is achieved like this:

This device comprises tank, baffle plate, new culture fluid, old culture fluid and bulb;

Tank is divided into two into left tank and right tank by being placed in the middle baffle plate of tank, in the middle of the bottom of baffle plate, is provided with aperture and rubber stopper thereof, and left tank is contained into new culture fluid, and right tank is poured old culture fluid and Daphnia magna into, on the left side of tank, is provided with bulb.

The present invention has following advantages and good effect:

1. in separation process, be that Daphnia magna is swum automatically to fresh medium, rather than conventional drawing with pipettor,

Avoided in suction process the individual issuable actual bodily harm of Daphnia magna;

2. the excreta of some swills in old culture fluid and Daphnia magna and cast off a skin etc. and substantially can not enter fresh culture fluid;

3. more conventional suction method has improved separative efficiency and disengaging time.

Accompanying drawing explanation

Fig. 1 is the structural representation of this device.

In figure:

00-Daphnia magna;

10-tank, 11-left tank, 12-right tank;

20-baffle plate, 21-aperture,, 22-rubber stopper;

30-new culture fluid;

40-old culture fluid;

50-bulb.

Embodiment

Below in conjunction with drawings and Examples, describe in detail:

one, change the device of culture fluid

1, overall

As Fig. 1, this device comprises tank 10, baffle plate 20, new culture fluid 30, old culture fluid 40 and bulb 50;

Tank 10 is divided into two into left tank 11 and right tank 12 by being placed in tank 10 middle baffle plates 20, in the middle of the bottom of baffle plate 20, be provided with aperture 21 and rubber stopper 22 thereof, left tank 11 is contained into new culture fluid 30, right tank 12 is poured old culture fluid 40 and Daphnia magna 00 into, on the left side of tank 10, is provided with bulb 50.

2, functional part

1) tank 10

Tank 10 is a kind of rectangular tank.

2) baffle plate 20

Baffle plate 20 is the dividing plate in the middle of tank 10.

Aperture 21 its diameter 5mm that arrange in the middle of baffle plate 20 bottoms, the size of rubber stopper 22 and aperture 21 adaptations.

3) new culture fluid 30

New culture fluid 30 is a kind of culture fluid that is conducive to Daphnia magna life;

The present invention adopts GB GB/B13266-91 < < water quality-material to flea class (Daphnia magna) determination of acute toxicity method > > configuration culture fluid.

4) old culture fluid 40

Old culture fluid 40 is the lived culture fluid of a period of time of Daphnia magna.

5) bulb 50

Bulb 50 is conventional bulb, and bulb 50 is selected 50W.

Two, change the method for culture fluid

This method comprises the following steps:

1. tank 10 is placed on the experimental bench of level, with rubber stopper 22, aperture 21 is blocked, make the water of left and right two

tanks

11,12 can not intercommunication;

2. need to change the Daphnia magna 00 of culture fluid and together pour in right tank 12 together with old culture fluid 40, then in tank 11, pour fresh medium 30 into left, and make the liquid level in left side exceed right side 1cm;

3. with light screening material, right tank 12 is carried out to shading, make it light tight;

4. left tank irradiates with light source 50;

5. the rubber stopper 22 of blocking aperture 21 is pulled out;

6. the Daphnia magna 00 in right tank 12 just can be swum tank 11 left by aperture 21 under the stimulation of illumination, and those Daphnia magna 00 cast off a skin and swill is just retained in right tank 12;

7. treat with rubber stopper 22, aperture 21 to be blocked again after Daphnia magna 00 shifts;

8. the newer culture fluid 30 in left tank 11 is transferred in culture vessel together with Daphnia magna 00.

Three, working mechanism

Daphnia magna has certain phototaxis to light source, like being gathered in the place that light source is more sufficient, when one of two of left and right tank is dark state for one of illumination state, Daphnia magna is just to there being the tank of illumination to assemble, and the baffle plate of two tanks centres is owing to there being an aperture, the tank that Daphnia magna just can independently be transferred to illumination by this aperture contains the tank of fresh medium, and because the excreta of Daphnia magna and casting off a skin etc. is static, therefore can stay original tank, thereby reach the object of efficiently changing culture fluid for Daphnia magna.

Four, test situation

This device is through indoor practical operation, finds finely compared with conventional separating method separating effect, and saved a lot of manpowers.

Claims

1. be the device that indoor cultivation Daphnia magna is changed culture fluid, it is characterized in that:

Comprise tank (10), baffle plate (20), new culture fluid (30), old culture fluid (40) and bulb (50);

Tank (10) is divided into two into left tank (11) and right tank (12) by being placed in the middle baffle plate (20) of tank (10), in the middle of the bottom of baffle plate (20), be provided with aperture (21) and rubber stopper (22) thereof, left tank (11) is contained into new culture fluid (30), right tank (12) is poured old culture fluid (40) and Daphnia magna (00) into, on the left side of tank (10), is provided with bulb (50).

2. the method for the replacing culture fluid based on device claimed in claim 1, is characterized in that comprising the following steps:

1. tank (10), on the experimental bench as for level, is blocked aperture (21) with rubber stopper (22), made the water of left and right two tanks (11,12) can not intercommunication;

2. need to change the Daphnia magna (00) of culture fluid and together pour in right tank (12) together with old culture fluid (40), then in tank (11), pour fresh medium (30) into left, and make the liquid level in left side exceed right side 1cm;

3. with light screening material, right tank (12) is carried out to shading, make it light tight;

4. left tank uses light source (50) to irradiate;

5. the rubber stopper (22) of blocking aperture (21) is pulled out;

6. the Daphnia magna (00) in right tank (12) just can be swum tank (11) left by aperture (21) under the stimulation of illumination, and those Daphnia magna (00) cast off a skin and swill is just retained in right tank (12);

7. treat to use again rubber stopper (22) that aperture (21) is blocked after Daphnia magna (00) shifts;

8. the newer culture fluid (30) in left tank (11) is transferred in culture vessel together with Daphnia magna (00).