CN107125186B - Large daphnia cultivation collection pool and collection method - Google Patents
Large daphnia cultivation collection pool and collection method Download PDFInfo
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- CN107125186B CN107125186B CN201710556507.9A CN201710556507A CN107125186B CN 107125186 B CN107125186 B CN 107125186B CN 201710556507 A CN201710556507 A CN 201710556507A CN 107125186 B CN107125186 B CN 107125186B
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- 238000000034 method Methods 0.000 title claims abstract description 11
- 241000238578 Daphnia Species 0.000 title description 6
- 241001494246 Daphnia magna Species 0.000 claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000009395 breeding Methods 0.000 claims abstract description 18
- 230000001488 breeding effect Effects 0.000 claims abstract description 18
- 238000005192 partition Methods 0.000 claims abstract description 11
- 239000011521 glass Substances 0.000 claims description 5
- 230000007704 transition Effects 0.000 claims description 5
- 238000000605 extraction Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000009602 toxicology test Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a daphnia magna breeding collection pool and a collection method, wherein the breeding collection pool comprises: breed district and enrichment district by the flashboard is separated, and the flashboard includes division board and sets up each graticule mesh in division board both sides, and the enrichment district is equipped with the light source, breeds the district and is provided with the shading lid that can cover breed district, and daphnia magna can freely pass through the mesh of graticule mesh, the division board can be followed and bred and gathered and pool internal extraction and separate, and the bottom in enrichment district is provided with the inlet outlet. The collecting method comprises the steps that S1, a light source in the enrichment area is lightened, the blackboard is arranged on the inner wall of the culture area, and the upper opening of the culture area is shielded by a shading cover of the culture area; s2, adjusting the water surface height in the enrichment area to keep the same as the water surface height in the culture area, and then drawing away the partition plate on the gate plate to enable the daphnia magna to freely pass through the grid; s3, feeding food baits into the enrichment area; s4 the divider plate is inserted back in place and separates the enrichment zone from the culture zone, and then the water in the enrichment zone is pumped out into a collection pond.
Description
Technical Field
The invention relates to a daphnia magna breeding and collecting technology, in particular to a daphnia magna breeding and collecting pool and a daphnia magna collecting method.
Background
The daphnia magna is widely used for research on pesticide environment safety evaluation and is widely applied to pesticide toxicology tests. The laboratory has a great demand for daphnia magna. A large amount of daphnia magna needs to be bred in a breeding mode for use in experiments, and meanwhile, the collection work after the daphnia magna is bred also needs to be finished efficiently. As various fermented materials and other substances are fed in the culture area, the interior of the culture area is dirty, the content of dirt is high, and the dirt and the daphnia magna need to be separated when the daphnia magna is collected.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a daphnia magna breeding collection pool and a collection method.
In order to achieve the purpose, the invention adopts a technical scheme that: a daphnia magna breeding collection pond, includes: breed district and enrichment district by the flashboard is separated, the flashboard includes the division board and sets up each graticule mesh of division board both sides, the enrichment district is equipped with the light source, breed the district and be provided with the shading lid that can cover breed the district, and daphnia magna can freely pass through the mesh of graticule mesh, the division board can be followed and bred and gather in the pond and take out, the bottom in enrichment district is provided with the inlet outlet.
In a preferred embodiment of the invention, the light source is a surface light source, and the surface light source is arranged on the pool wall of the enrichment area.
In a preferred embodiment of the invention, the culture collection pond is made of glass, and the light source is arranged on the outer wall of the culture collection pond.
In a preferred embodiment of the invention, the blackboard is detachably arranged on the wall of the culture area.
In a preferred embodiment of the invention, under cultivation conditions, the separation plate on the gate plate separates the cultivation area from the collection area, and daphnia magna cannot pass through the cultivation area to the enrichment area.
In a preferred embodiment of the invention, after the partition plate is pulled away, a transition space is formed between the two layers of grids.
In a preferred embodiment of the present invention, the wavelength range of the light source is 460nm-470 nm.
In a preferred embodiment of the invention, a reflective cover is arranged at the upper opening of the enrichment area.
The other technical scheme adopted by the invention is as follows: a method for collecting daphnia magna in a culture collection pond comprises the following steps:
s1, lighting a light source in the enrichment area, installing a blackboard on the inner wall of the culture area, and shielding the upper opening of the culture area by a shading cover of the culture area;
s2, adjusting the water surface height in the enrichment area to keep the same as the water surface height in the culture area, and then drawing away the partition plate on the gate plate to enable the daphnia magna to freely pass through the grid;
s3, feeding food baits into the enrichment area;
s4 the divider plate is inserted back in place and separates the enrichment zone from the culture zone, and then the water in the enrichment zone is pumped out into a collection pond.
In a preferred embodiment of the present invention, the duration of step S3 is 15 minutes to 150 minutes.
In a preferred embodiment of the invention, food bait is placed every 15 to 30 minutes.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) the enrichment area can attract the daphnia magna to realize enrichment and collection of the daphnia magna through light and food baits, meanwhile, the daphnia magna is separated from relatively disordered breeding feed in the breeding area, the collected daphnia magna is relatively clean, and the step of consuming a large amount of energy to remove incoming impurity dirt after collection is omitted.
(2) The area source sets up outside the pool wall in enrichment district, and the separation of light source realization and water, light source need not higher waterproof characteristic, and glass's pool wall has realized the perspective of light simultaneously, has realized that light guide large-scale daphnia floats to enrichment district.
(3) The shading cover and the blackboard are arranged corresponding to the light source, and provide shading conditions for the culture area, and the light source function of the enrichment area is reflected, so that the light condition contrast of the enrichment area and the culture area is relatively high, and the function of light luring daphnia is embodied as much as possible; the reflecting cover ensures that the intensity of light in the enrichment area is higher.
(4) The water inlet and the water outlet can control the liquid level height in the enrichment area and the culture area, the culture area and water in the enrichment area are kept static relatively after the flashboard is moved away, dirt in the culture area is not easy to flow to the enrichment area, meanwhile, the double-layer grid net can isolate the dirt to enter the enrichment area to a certain degree, and the transition space can keep the enrichment area from entering too much dirt to a certain degree.
(5) The water inlet and the water outlet can completely pump out water in the enrichment area at one time after the enrichment is finished, the water enriches a large amount of daphnia magna for later use after dirt is basically removed, and excessive workload is not needed when a small amount of dirt is removed on the basis.
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a schematic top view of a preferred embodiment of the present invention;
in the figure: 1. a breeding area; 2. an enrichment zone; 3. a surface light source; 4. a blackboard; 5. a partition plate; 6. grid forming; 7. a water inlet and a water outlet.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
As shown in fig. 1, a daphnia magna cultivation collection pool comprises: breed district and enrichment district by the flashboard is separated, the flashboard includes the division board and sets up each graticule mesh of division board both sides, the enrichment district is equipped with the light source, it is provided with the shading lid that can cover the district of breeding to breed the district, and daphnia magna can freely pass through the mesh of graticule mesh, the division board can be followed and bred and gathered taking pond and take out, and the enrichment district can lure daphnia magna to realize the enrichment of daphnia magna through light and food bait and gather, has also realized the separation of daphnia magna and the comparatively mixed and disorderly breed raiser in the district simultaneously, and the daphnia magna of gathering is cleaner, has removed the step that consumes a large amount of energy to clear.
The bottom in enrichment district is provided with the inlet outlet, and the liquid level height in enrichment district and the breed district can be controlled to the inlet outlet, guarantees that the flashboard is moving aside the back, and the breed district keeps static with the water in the enrichment district relatively, guarantees that the filth in the breed district is difficult to flow to the enrichment district, and double-deck graticule mesh can keep apart the filth to a certain extent simultaneously and get into the enrichment district, and the transition space can keep the enrichment district to a certain extent can not get into too much filth.
The water inlet and the water outlet can completely pump out water in the enrichment area at one time after the enrichment is finished, the water enriches a large amount of daphnia magna for later use after dirt is basically removed, and excessive workload is not needed when a small amount of dirt is removed on the basis.
The light source is a surface light source, the surface light source is arranged on the wall of the enrichment area, the culture collection pool is made of glass, and the light source is arranged on the outer wall of the culture collection pool. The area source sets up outside the pool wall in enrichment district, and the separation of light source realization and water, light source need not higher waterproof characteristic, and glass's pool wall has realized the perspective of light simultaneously, has realized that light guide large-scale daphnia floats to enrichment district.
The blackboard is detachably arranged on the wall of the culture area. The shading cover and the blackboard are arranged corresponding to the light source, and provide shading conditions for the culture area, and the light source function of the enrichment area is reflected, so that the light condition contrast of the enrichment area and the culture area is relatively high, and the function of light luring daphnia is embodied as much as possible; the reflecting cover ensures that the intensity of light in the enrichment area is higher.
Under the cultivation condition, the division plate on the gate plate separates the cultivation area from the collection area, and the daphnia magna cannot pass through the enrichment area from the cultivation area.
After the partition plate is pulled away, a transition space is formed between the two layers of grids, and the wavelength range of the light source is 460-470 nm. A reflective cover is arranged at the upper opening of the enrichment area.
A method for collecting daphnia magna in a culture collection pond comprises the following steps:
s1, lighting a light source in the enrichment area, installing a blackboard on the inner wall of the culture area, and shielding the upper opening of the culture area by a shading cover of the culture area;
s2, adjusting the water surface height in the enrichment area to keep the same as the water surface height in the culture area, and then drawing away the partition plate on the gate plate to enable the daphnia magna to freely pass through the grid;
s3, feeding food baits into the enrichment area;
s4 the divider plate is inserted back in place and separates the enrichment zone from the culture zone, and then the water in the enrichment zone is pumped out into a collection pond.
Step S3 lasts 15-150 minutes, and food bait is placed every 15-30 minutes.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (6)
1. A daphnia magna breeding collection pond, includes: the device comprises a breeding area and an enrichment area which are separated by a gate plate, and is characterized in that the gate plate comprises a partition plate and grid nets arranged on two sides of the partition plate, the enrichment area is provided with a light source, the breeding area is provided with a shading cover capable of covering the breeding area, daphnia magna can freely pass through the meshes of the grid nets, the partition plate can be extracted from a breeding collection pool, and the bottom of the enrichment area is provided with a water inlet and a water outlet;
the light source is a surface light source which is arranged on the wall of the enrichment region;
a blackboard is detachably arranged on the wall of the culture area;
under the cultivation condition, the division plate on the gate plate separates the cultivation area from the collection area, and the daphnia magna cannot pass through the cultivation area to the enrichment area;
after the partition plate is pulled away, a transition space is formed between the two layers of grids.
2. The daphnia magna cultivation collection pool of claim 1, wherein: the breeding collection pool is made of glass, and the light source is arranged on the outer wall of the breeding collection pool.
3. The daphnia magna cultivation collection pool of claim 1, wherein: the wavelength range of the light source is 460nm-470 nm.
4. A method for collecting daphnia magna in a culture collection pond according to any one of claims 1-3, comprising the steps of:
s1, lighting a light source in the enrichment area, installing a blackboard on the inner wall of the culture area, and shielding the upper opening of the culture area by a shading cover of the culture area;
s2, adjusting the water surface height in the enrichment area to keep the same as the water surface height in the culture area, and then drawing away the partition plate on the gate plate to enable the daphnia magna to freely pass through the grid;
s3, feeding food baits into the enrichment area;
s4 the divider plate is inserted back in place and separates the enrichment zone from the culture zone, and then the water in the enrichment zone is pumped out into a collection pond.
5. The method of acquisition as claimed in claim 4, wherein: step S3 lasts 15-150 minutes.
6. The method of acquisition as claimed in claim 5, wherein: food baits were placed every 15 to 30 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710556507.9A CN107125186B (en) | 2017-07-10 | 2017-07-10 | Large daphnia cultivation collection pool and collection method |
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| CN201710556507.9A CN107125186B (en) | 2017-07-10 | 2017-07-10 | Large daphnia cultivation collection pool and collection method |
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| CN107125186A CN107125186A (en) | 2017-09-05 |
| CN107125186B true CN107125186B (en) | 2020-11-24 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667930A (en) * | 2017-11-03 | 2018-02-09 | 山东省农药科学研究院 | It is easy to gather the breeding spaces and acquisition method of Daphnia magna |
| CN108056052A (en) * | 2018-01-16 | 2018-05-22 | 广东中科英海科技有限公司 | Daphnia magna culture collection device |
| CN111323553A (en) * | 2020-03-13 | 2020-06-23 | 浙江省海洋水产研究所 | A pond of breeding fish for toxicological experiment |
| CN112129908A (en) * | 2020-09-29 | 2020-12-25 | 长江大学 | Device and method for analyzing toxicity of high-salinity wastewater by utilizing daphnia magna phototaxis |
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