CN111323553A - A pond of breeding fish for toxicological experiment - Google Patents

A pond of breeding fish for toxicological experiment Download PDF

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Publication number
CN111323553A
CN111323553A CN202010176458.8A CN202010176458A CN111323553A CN 111323553 A CN111323553 A CN 111323553A CN 202010176458 A CN202010176458 A CN 202010176458A CN 111323553 A CN111323553 A CN 111323553A
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China
Prior art keywords
pond
experiment
fish
turnover
experimental
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CN202010176458.8A
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Chinese (zh)
Inventor
王范盛
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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Priority to CN202010176458.8A priority Critical patent/CN111323553A/en
Publication of CN111323553A publication Critical patent/CN111323553A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18Water
    • G01N33/186Water using one or more living organisms, e.g. a fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a fish pond for toxicological experiments, which comprises an adjacent experiment pond and a turnover pond, wherein a break-make gate is arranged between the experiment pond and the turnover pond, a fish driving mesh plate is arranged in the experiment pond, and the fish driving mesh plate is connected between the experiment pond and the turnover pond in a sliding manner. When the invention is used for replacing pond water and medicament in the toxicological experiment process, the tested fish does not need to leave water, the damage to the fish can be reduced, the tested fish can be kept full activity for subsequent experiments, and the accuracy of the experiment result is ensured.

Description

A pond of breeding fish for toxicological experiment
Technical Field
The invention relates to a culture facility, in particular to a fishpond for toxicological experiments.
Background
The fish has sensitive response to the change of water environment, and when pollutants in the water reach a certain degree, a series of toxic reactions, such as abnormal behaviors, physiological dysfunction and histiocytosis, can be caused until the fish dies. With the continuous advance of industrialization, a large amount of industrial wastewater is discharged into rivers and lakes, so that natural water is seriously polluted, and the drinking water safety of human beings is greatly threatened. Therefore, the country has come out of stricter environmental protection policies, and many environmental protection measures are gradually strengthened, and water quality monitoring of natural waters is one of the policies. In water environment monitoring, fish toxicity experiments are applied more, and the main contents are as follows: the tested fishes are put into water bodies with known or unknown toxicants in different concentrations, and various reactions and survival conditions of the fishes are recorded and observed. The fish toxicity test can reflect the comprehensive pollution condition of the water body and the combined toxicity of pollutants to a certain extent, can provide a basis for chemical registration and fishery water quality standards, and has the advantage of irreplaceable physicochemical monitoring. Some technical equipment related to fish toxicological experiments also appear in the market, but the existing fish toxicological experiment equipment is deficient in design, the necessary protection to the tested fish during water and medicament changing is neglected, water in a pool is usually directly drained during operation, or the tested fish is fished out, so that the fish can be separated from water for a long time or a short time during the water and medicament changing process, and constraint force or oppression force can be exerted on the fish, which can cause frightening or injury to the fish, is not beneficial to the tested fish to keep full vitality for subsequent experiments, and is not beneficial to ensuring the accuracy of the experiment result. The utility model discloses a public number is CN209542597U specially does benefit to 2019 and 10 months 25 and discloses a fish toxicology experimental apparatus, including base, riser, experiment bucket, riser fixed mounting is on the base, the experiment bucket passes through the connecting seat and installs on the riser, the upper end fixedly connected with roof of riser, the through-hole has been seted up on the roof, the through-hole internal fixation is inserted and is equipped with the cavity standpipe, the lower extreme of cavity standpipe stretches into in the experiment bucket and installs a plurality of capillary transfer lines, lay the venom bin on the roof, one side of venom bin is provided with the pump of laying on the roof, the input of pump is connected with the venom bin, the output of pump is connected with the upper end of cavity standpipe, the interior bottom of experiment bucket homogenizes and is provided with the mechanism. But what this utility model solved is that improve the technical problem of the filling degree of consistency of venom in the water in the experiment bucket to can not solve the problem that reduces the surprise or injury to the fish of experimenting in the experimentation.
Disclosure of Invention
The fish toxicology experiment equipment in the prior art has the defects in design, can cause frightening or damage to the fish when water and medicaments are changed, is not beneficial to keeping sufficient activity of the tested fish for subsequent experiments, and is not beneficial to ensuring the accuracy of experiment results.
The technical scheme of the invention is as follows: the utility model provides a fishpond for toxicological experiment, includes adjacent experiment pond and turnover pond, is equipped with the break-make gate between experiment pond and turnover pond, is equipped with in the experiment pond and drives fishnet orifice plate, drives fishnet orifice plate sliding connection in the experiment pond. When the device is used for carrying out fish toxicity experiments, tested fishes are left in the experiment pool, the on-off gate is closed, the experiment pool is separated from the turnover pool, the fish mesh plate is driven to slide to the position farthest away from the turnover pool, then a toxic agent to be detected is added into the experiment pool, and the reaction and survival conditions of the tested fishes are observed and recorded. If the change is waited to examine toxicity medicament kind, original pond water and medicament in the experiment pond need be drained, open break-make gate this moment, make experiment pond and turnover pond intercommunication, slide again and drive the fishnet orifice plate and be close to the turnover pond gradually, force the fish of receiving the test to get into the turnover pond, then close break-make gate once more, make the fish of receiving the test stay in the turnover pond. At the moment, original pond water and chemicals in the experiment pond are discharged, new pond water is added, the turnover pond simultaneously drains water and supplies water, the experiment pond and the turnover pond independently change water respectively, and finally, the replacement of the pond water in the experiment pond and the turnover pond is completed. After water is changed, the fish driving mesh plate moves back to the position farthest away from the turnover pool, the on-off gate is opened again, and a new fish toxicity experiment can be carried out continuously. In the process of changing pond water and medicament, the tested fish does not need to leave water, so that the damage to the fish is reduced, the tested fish is favorable for keeping full activity so as to carry out subsequent experiments, and the accuracy of experimental results is ensured.
Preferably, the wall of the experimental pond is provided with a sliding chute, the fish driving mesh plate is connected to the sliding chute in a sliding manner, and the fish driving mesh plate is connected with a driving mechanism. The spout can be ensured to drive the fish net orifice plate and along setting for the stable removal in route, realizes driving the sliding connection of fish net orifice plate between experiment pond and turnover pond, drives the fish net orifice plate and is driven by actuating mechanism, can practice thrift the manual work, improves experimental efficiency.
Preferably, the driving mechanism comprises a motor and a screw rod parallel to the sliding groove, the motor is fixed at the end of the experiment pool, the screw rod is connected to the output end of the motor, and the fish-driving mesh plate is in threaded connection with the screw rod. The motor runs to drive the screw rod, and the relative rotation between the screw rod and the fish driving mesh plate is converted into relative axial motion, so that driving force is provided for the sliding of the fish driving mesh plate on the sliding chute.
Alternatively, the driving mechanism comprises a motor, a driving chain wheel, a driven chain wheel and a transmission chain, the motor is fixed at the end part of the experiment pool, the driving chain wheel is connected at the output end of the motor, the transmission chain is connected between the driving chain wheel and the driven chain wheel, and the fish-driving mesh plate is fixed on the transmission chain. The motor operates to drive the driving chain wheel and further drive the transmission chain, the transmission chain provides driving force for the sliding of the fish driving mesh plate on the sliding groove, and the fish driving mesh plate can do linear motion along the sliding groove along with the rotation of the transmission chain.
Preferably, a turnover pool water inlet and outlet pipe is arranged on the turnover pool. The arrangement of the water inlet and outlet pipes of the turnover pool facilitates independent pool water replacement of the turnover pool.
Preferably, the experiment pools are uniformly distributed around the turnover pool. A plurality of experiment pools share a turnover pool, can carry out the toxicological experiment of multiple toxicity medicament simultaneously, improve experimental efficiency.
Preferably, the experiment pool is provided with an experiment pool water inlet and outlet pipe and a toxic agent feeder. The water inlet and outlet pipes of the experiment pool are used for water supply and drainage of the experiment pool, so that the water in the experiment pool can be conveniently replaced. The toxic medicament feeder is used for concentrated feeding of toxic medicaments, and is convenient for accurate metering.
Preferably, the bottom of the experimental tank is provided with a stirrer, and the stirrer is sunk into the bottom of the experimental tank. When the stirrer runs, the water in the experiment pool is stirred to flow, and the toxic medicament is added to accelerate the uniform diffusion. The agitator sinks into the bottom of the pool, and interference can not be formed on the movement of the fish driving mesh plate.
Preferably, the experiment pool and the turnover pool are both connected with a water supply tank. Experiment pond and turnover pond follow same water source water intaking, can ensure that the experimental water in different times, different ponds has physicochemical parameters such as same temperature, pH valve, impurity content for the experiment in different times, different ponds has comparability, guarantees the experiment accuracy.
The invention has the beneficial effects that:
reducing the damage to the tested fish. When the invention is used for replacing pond water and medicament in the toxicological experiment process, the tested fish does not need to leave water, the damage to the fish can be reduced, the tested fish can be kept full activity for subsequent experiments, and the accuracy of the experiment result is ensured.
Drawings
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is an internal structural view of the present invention;
FIG. 3 is a schematic diagram of another layout of the experimental tank and the turnover tank in the present invention;
fig. 4 is another internal structural view of the present invention.
In the figure, 1-an experimental tank, 2-a turnover tank, 3-an on-off gate, 4-a fish-driving mesh plate, 5-a chute, 6-a motor, 7-a screw rod, 8-a driving chain wheel, 9-a driven chain wheel, 10-a transmission chain, 11-a stirrer, 12-a water supply box, 13-a toxic agent feeder, 14-a turnover tank water inlet pipe, 15-a turnover tank water outlet pipe, 16-an experimental tank water inlet pipe, 17-an experimental tank water outlet pipe, 18-a gate lifting motor, 19-a communication port and 20-a gate lifting screw rod.
Detailed Description
The invention is further illustrated by the following specific embodiments in conjunction with the accompanying drawings.
Example 1:
as shown in figures 1 and 2, the fishpond for toxicological experiments comprises four adjacent experiment ponds 1 and turnover ponds 2, wherein the turnover ponds 2 are one, the experiment ponds 1 are uniformly distributed around the turnover ponds 2 and are arranged into a cross shape, the experiment ponds 1 are rectangular, and the turnover ponds 2 are square. A rectangular communication port 19 and an on-off gate 3 are arranged between the experiment pool 1 and the turnover pool 2, gate sliding grooves are formed in the two sides of the communication port 19 and the inner wall of the top of the communication port, the on-off gate 3 is connected in the gate sliding grooves in a sliding mode, and a sealing ring is arranged on the inner peripheral surface of the communication port 19. Be equipped with the nut piece near 19 departments of intercommunication mouth in the turnover pond 2, 2 pond wall tops in the turnover pond are equipped with gate elevator motor 18, there is gate lifting screw 20 gate elevator motor 18 output through the coupling joint, the nut piece threaded connection in gate lifting screw 20 and the turnover pond 2, be fixed with the connecting block on the break-make gate 3, be equipped with the shaft hole on the connecting block, the shaft hole rotates with 20 bottom unthreaded position of gate lifting screw to be connected and gate lifting screw 20 bottom global on the joint have a pair of retaining ring for the shaft, the retaining ring for the shaft pastes respectively on the connecting block top, two sides at the bottom, the diameter of retaining ring for the shaft is greater than the shaft hole aperture, it is spacing to make to realize the axial between connecting block and gate lifting. In the lifting process of the on-off gate 3, the right end face of the on-off gate 3 is in contact with the sealing ring, and when the on-off gate 3 is lowered to the bottom, the right end face of the on-off gate 3 is completely attached to the sealing ring to completely seal the communication port 19. Be equipped with in the experiment pond 1 and drive fishnet hole board 4, drive the dense mesh on fishnet hole board 4 for the rivers can pass the mesh when driving fishnet hole board 4 and removing, drive fishnet hole board 4 sliding connection in experiment pond 1. The well upper portion of the relative inner wall of pool wall of experiment pond 1 is equipped with spout 5, and the T shape is personally submitted in the 5 cross sections of spout, drives the slider that fishnet hole board 4 both sides were equipped with the T shape, and slider adaptation slides and inlays in spout 5 for drive fishnet hole board 4 and 1 inner wall sliding connection in experiment pond, drive fishnet hole board 4 and be connected with a actuating mechanism. The actuating mechanism includes motor 6 and the screw rod 7 parallel with spout 5, and motor 6 passes through the motor support to be fixed in experiment 1 outer end of pond, keeps away from the central authorities of the one end in turnover pond 2 promptly, and 7 one ends of screw rod are passed through the coupling joint and are being connected at the output of motor 6, and the screw rod 7 other end passes through the bearing and rotates the connection on experiment 1 interior end wall. The motor 6 and the gate lifting motor 18 are controlled by switch buttons. The center of the top of the fish driving mesh plate 4 is fixedly provided with a nut block, and the nut block is in threaded connection with the screw 7. The wall of the turnover pool 2 is provided with a turnover pool water inlet pipe 14 and a turnover pool water outlet pipe 15. Water valves are arranged on the water inlet pipe 14 and the water outlet pipe 15 of the turnover tank. Be equipped with experiment pond inlet tube 16, experiment pond outlet pipe 17 and toxicity medicament feeder 13 on the 1 pond wall in experiment pond, all be equipped with the water valve on experiment pond inlet tube 16 and the experiment pond outlet pipe 17, be equipped with open and close valve and scale on the toxicity medicament feeder 13, be convenient for put in according to the dose is accurate. The bottom of the experimental tank 1 is provided with a stirrer 11, and the stirrer 11 sinks into a pit at the bottom of the tank. The experiment tank 1 and the turnover tank 2 are both connected with a water supply tank 12, and a turnover tank inlet pipe 14 and an experiment tank inlet pipe 16 are connected at the water outlet end of the water supply tank 12.
The fish toxicity test carried out by using the invention comprises the following steps: the tested fish is left in the experimental tank 1, and the on-off gate 3 is closed to separate the experimental tank 1 from the turnover tank 2; starting a motor 6 to drive a screw rod 7, converting the relative rotation between the screw rod 7 and the fish driving mesh plate 4 into relative axial movement, thereby providing driving force for the sliding of the fish driving mesh plate 4 on the sliding chute 5, enabling the fish driving mesh plate 4 to slide to the farthest position away from the turnover tank 2, then adding a toxic agent to be detected into the experimental tank 1, observing and recording the reaction and survival conditions of the tested fish; if the type of the toxic agent to be detected is changed, the on-off gate 3 is opened to communicate the experiment pool 1 with the turnover pool 2, then the fish mesh plate 4 is driven to gradually approach the turnover pool 2 in a sliding manner, the tested fish is forced to enter the turnover pool 2, and then the on-off gate 3 is closed again to enable the tested fish to remain in the turnover pool 2; original pond water and medicament in the discharge experiment pond 1 to add new pond water, have enough to meet need the pond 2 simultaneously and also carry out drainage and feedwater in step, experiment pond 1, have enough to meet need the pond 2 and independently change water separately, finally accomplish the old and new change of pond water in experiment pond 1, the turnover pond 2. The water injection depth of the experimental pond 1 is controlled below a median line of the inner wall of the experimental pond 1, a safe distance is kept between the experimental pond and the driving mechanism, and the motor 6 is prevented from being stained with water. After water is changed, the starting motor 6 is reversely rotated to drive the fish mesh plate 4 to move back to the position farthest away from the turnover pool 2, the on-off gate 3 is opened again, and a new fish toxicity experiment can be continuously carried out.
Example 2:
as shown in fig. 3 and 4, the number of the experimental pools 1 is three, the number of the turnover pools 2 is one, the experimental pools 1 are uniformly distributed around the turnover pools 2 and arranged in a Y shape, the experimental pools 1 are rectangular, and the turnover pools 2 are regular triangles. The driving mechanism comprises a motor 6, a driving chain wheel 8, a driven chain wheel 9 and a transmission chain 10, the motor 6 is fixed at the outer end of the top of the experiment pool 1, the driving chain wheel 8 is connected at the outer end of the top of the experiment pool 1 through the rotation of a wheel carrier, the driven chain wheel 9 is connected at the inner end of the top of the experiment pool 1 through the rotation of the wheel carrier, the driving chain wheel 8 is connected with the output end of the motor 6 in a key mode, the transmission chain 10 is connected between the driving chain wheel 8 and the driven chain wheel 9, and the fish mesh plate. The rest is the same as example 1.
When the motor 6 runs, the driving chain wheel 8 is driven, and then the driving chain 10 is driven, the driving chain 10 provides driving force for the sliding of the fish mesh plate 4 on the sliding groove 5, and the fish mesh plate 4 can move linearly along the sliding groove 5 along with the rotation of the driving chain 10.
Example 3:
experiment pond 1 is two, has enough to meet the need pond 2 and is one, and 1 symmetrical arrangement in experiment pond is arranged into the straight line at 2 both ends in the turnover pond, and experiment pond 1 is the rectangle, and turnover pond 2 is the square. The rest is the same as example 1.

Claims (9)

1. The utility model provides a fishpond for toxicological experiment, characterized by includes adjacent experiment pond (1) and turnover pond (2), is equipped with break-make gate (3) between experiment pond (1) and turnover pond (2), is equipped with in experiment pond (1) and drives fishnet hole board (4), drives fishnet hole board (4) sliding connection in experiment pond (1).
2. The fishpond for toxicological experiments according to claim 1, wherein the wall of the experimental pond (1) is provided with a chute (5), the fish-driving mesh plate (4) is slidably connected to the chute (5), and the fish-driving mesh plate (4) is connected with a driving mechanism.
3. The fishpond for toxicology experiments according to claim 2, wherein the driving mechanism comprises a motor (6) and a screw (7) parallel to the chute (5), the motor (6) is fixed at the end of the experimental pond (1), the screw (7) is connected at the output end of the motor (6), and the fish-driving mesh plate (4) is in threaded connection with the screw (7).
4. The fishpond for toxicology experiments according to claim 2, wherein the driving mechanism comprises a motor (6), a driving sprocket (8), a driven sprocket (9) and a transmission chain (10), the motor (6) is fixed at the end of the experimental pond (1), the driving sprocket (8) is connected at the output end of the motor (6), the transmission chain (10) is connected between the driving sprocket (8) and the driven sprocket (9), and the fish-driving mesh plate (4) is fixed on the transmission chain (10).
5. The fishpond for toxicological experiments according to claim 1, wherein the turnover pond (2) is provided with a turnover pond inlet and outlet pipe.
6. The fishpond for toxicological experiments according to claim 1, wherein the experimental pond (1) is provided in plurality, and the experimental pond (1) is uniformly distributed around the turnover pond (2).
7. The fishpond for toxicological experiments according to claim 1, wherein the experimental pond (1) is provided with an experimental pond water inlet and outlet pipe and a toxic agent feeder (13).
8. A fish pond for toxicological experiments according to any one of claims 1 to 7, wherein the bottom of the experimental pond (1) is provided with a stirrer (11), and the stirrer (11) is sunk into the bottom of the pond.
9. Fishpond for toxicological experiments according to any one of claims 1 to 7, characterized in that the experimental tank (1) and the turnaround tank (2) are connected to a water supply tank (12).
CN202010176458.8A 2020-03-13 2020-03-13 A pond of breeding fish for toxicological experiment Pending CN111323553A (en)

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CN202010176458.8A CN111323553A (en) 2020-03-13 2020-03-13 A pond of breeding fish for toxicological experiment

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CN111323553A true CN111323553A (en) 2020-06-23

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CN102630620A (en) * 2012-04-27 2012-08-15 三峡大学 Screening device for fishes of particular specification
CN103416340A (en) * 2013-07-12 2013-12-04 深圳市福田区青少年科技教育协会 Automatic water changing fish tank
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CN110063275A (en) * 2019-03-04 2019-07-30 中国环境科学研究院 A kind of zebra fish cultural method stage by stage
CN110074037A (en) * 2019-05-22 2019-08-02 河海大学 Pollutant is to fish toxicological effect research device
CN110122115A (en) * 2019-06-26 2019-08-16 浙江大学城市学院 Balcony fish and vegetable symbiotic system
CN110150186A (en) * 2019-06-14 2019-08-23 中国水产科学研究院黑龙江水产研究所 A kind of integrated experimental device and operating method for toxicity in fish research

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201905152U (en) * 2010-11-23 2011-07-27 中国科学院广州地球化学研究所 Automatic flowing water change devices for toxicity test of aquatic animals
CN102630620A (en) * 2012-04-27 2012-08-15 三峡大学 Screening device for fishes of particular specification
CN103416340A (en) * 2013-07-12 2013-12-04 深圳市福田区青少年科技教育协会 Automatic water changing fish tank
CN104016010A (en) * 2014-04-04 2014-09-03 浙江省海洋水产研究所 Chilling preservation box for aquatic products
CN205124731U (en) * 2015-11-04 2016-04-06 中国水产科学研究院长江水产研究所 Experimental device for be used for studying medicine at fish body materialbalance
CN105557608A (en) * 2015-12-21 2016-05-11 同济大学 Aquatic animal toxicology experiment system and application
CN205511698U (en) * 2016-04-26 2016-08-31 中国水产科学研究院黑龙江水产研究所 Experimental device for be used for studying fish urgency, chronic toxicity
CN205865643U (en) * 2016-07-12 2017-01-11 浙江省海洋水产养殖研究所 Aquatic livestock running water breeding experimental apparatus
CN106719242A (en) * 2016-11-24 2017-05-31 防城港市畜牧站 A kind of dual chamber aquaculture fishpond
CN206851765U (en) * 2017-02-21 2018-01-09 芮朝利 A kind of fishpond of convenient fishing
CN107125186A (en) * 2017-07-10 2017-09-05 佛山市水创科联生态科技有限公司 Daphnia magna cultivation collection pond and acquisition method
CN107810901A (en) * 2017-11-07 2018-03-20 新希望六和饲料股份有限公司 A kind of feed is metabolized collection fish jar
CN207428227U (en) * 2017-11-27 2018-06-01 中国水产科学研究院黑龙江水产研究所 A kind of fish toxicological experiment device
CN110063275A (en) * 2019-03-04 2019-07-30 中国环境科学研究院 A kind of zebra fish cultural method stage by stage
CN110074037A (en) * 2019-05-22 2019-08-02 河海大学 Pollutant is to fish toxicological effect research device
CN110150186A (en) * 2019-06-14 2019-08-23 中国水产科学研究院黑龙江水产研究所 A kind of integrated experimental device and operating method for toxicity in fish research
CN110122115A (en) * 2019-06-26 2019-08-16 浙江大学城市学院 Balcony fish and vegetable symbiotic system

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Application publication date: 20200623