CN110150186A - A kind of integrated experimental device and operating method for toxicity in fish research - Google Patents
A kind of integrated experimental device and operating method for toxicity in fish research Download PDFInfo
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- CN110150186A CN110150186A CN201910514834.7A CN201910514834A CN110150186A CN 110150186 A CN110150186 A CN 110150186A CN 201910514834 A CN201910514834 A CN 201910514834A CN 110150186 A CN110150186 A CN 110150186A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 22
- 231100000419 toxicity Toxicity 0.000 title claims abstract description 16
- 230000001988 toxicity Effects 0.000 title claims abstract description 16
- 238000011160 research Methods 0.000 title claims abstract description 15
- 238000011017 operating method Methods 0.000 title claims abstract description 6
- 239000000243 solution Substances 0.000 claims abstract description 85
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 62
- 238000006243 chemical reaction Methods 0.000 claims abstract description 52
- 239000011550 stock solution Substances 0.000 claims abstract description 26
- 238000005273 aeration Methods 0.000 claims abstract description 15
- 238000002474 experimental method Methods 0.000 claims description 34
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 29
- 229910052760 oxygen Inorganic materials 0.000 claims description 29
- 239000001301 oxygen Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 11
- 238000012612 static experiment Methods 0.000 claims description 9
- 238000005276 aerator Methods 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 5
- 238000009830 intercalation Methods 0.000 claims description 5
- 230000002687 intercalation Effects 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 4
- 238000006298 dechlorination reaction Methods 0.000 claims description 3
- 238000013401 experimental design Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 description 8
- 231100000027 toxicology Toxicity 0.000 description 6
- 239000003344 environmental pollutant Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 231100000719 pollutant Toxicity 0.000 description 5
- 230000002110 toxicologic effect Effects 0.000 description 3
- 230000001195 anabolic effect Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 231100001252 long-term toxicity Toxicity 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000001473 noxious effect Effects 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A kind of integrated experimental device and operating method for toxicity in fish research, including stock solution case, dilute solution case, reaction solution case, temperature controller, temperature sensor, aeration pump, automatic controller, water tank, the stock solution case connects the first peristaltic pump side by pipeline, first peristaltic pump other side connects reaction solution case by pipeline, first time relay is provided on first peristaltic pump, the dilute solution case connects the second peristaltic pump side by pipeline, second peristaltic pump other side connects reaction solution case by pipeline, second time relay is provided on second peristaltic pump, the reaction solution case right end is sequentially connected third peristaltic pump by pipeline, spinner flowmeter, water inlet, the third time relay is provided on the third peristaltic pump, the water inlet right end attended operation case.
Description
Technical field
The present invention relates to ecosphere more particularly to a kind of integrated experimental devices and operation for toxicity in fish research
Method.
Background technique
Aquatic ecological toxicology is always the important component in ecological toxicology field.Using fish grinding as experimental animal
Study carefully the attention in recent years increasingly by toxicology field researcher, not only probes into pollutant to the toxicity of aquatile
Important function is played in effect and its mechanism of action, relevant data additionally aid evaluation pollutant to mammal or even people
The influence of class health.Fish toxicological experiment is more stringent to experimental material and condition requirement, need to be according to the spy of different pollutants
Point selects different experimental methods, and because of the different psychological needs for examination biology, needs strict control water temperature and dissolved oxygen etc.
Water quality indicator.The device that development fish toxicological experiment uses at present is respectively constructed without unified standard, complex for operation step, is accounted for
Use the plenty of time.So there has been no more satisfactory experimental provisions at present.
Summary of the invention
The present invention In view of the above shortcomings of the prior art, it is malicious for fish to provide a kind of one kind for controlling water quality indicator
The integrated experimental device of Journal of Sex Research.
The purpose of the present invention is be achieved through the following technical solutions:
A kind of integrated experimental device for toxicity in fish research, including stock solution case, dilute solution case, reaction solution
Case, temperature controller, temperature sensor, aeration pump, automatic controller, water tank, the stock solution case pass through pipeline connection first
Peristaltic pump side, first peristaltic pump other side connect reaction solution case by pipeline, are provided on first peristaltic pump
First time relay, the dilute solution case connect the second peristaltic pump side, second peristaltic pump other side by pipeline
Reaction solution case is connected by pipeline, second time relay is provided on second peristaltic pump, the reaction solution case is right
End is sequentially connected third peristaltic pump, spinner flowmeter, water inlet by pipeline, is provided with the third time on the third peristaltic pump
Relay, the water inlet right end attended operation case, the water inlet are located at control box upper left quarter, connection on the right side of the control box
Water outlet, the water outlet are located at control box right lower quadrant, and the water outlet right end connects the 4th peristaltic pump side, institute by pipeline
It states the 4th peristaltic pump other side and water tank is connected by pipeline, be provided with the 4th time relay on the 4th peristaltic pump, institute
Stating has multiple sample taps on the upside of control box, fill reaction solution inside the control box.
Insulating layer is connected on the outside of the control box, heater is set on the inside of the insulating layer, the heater passes through conducting wire
Temperature controller is connected, the temperature controller is by conducting wire connection temperature sensor, in the temperature sensor lower end intercalation reaction solution,
The temperature sensor is arranged on the left of control box.
The automatic controller connects aeration pump side, and the aeration pump other side connects micro-hole aerator, the micropore
Aerator is arranged in control box bottom.
The automatic controller connects dissolved oxygen sensor by conducting wire, and dissolved oxygen sensor lower end intercalation reaction is molten
In liquid, the dissolved oxygen sensor is arranged on the right side of control box.
A kind of integrated experimental device operating method for toxicity in fish research:
Stock solution is packed into stock solution case, stock solution is the highly concentrated solution containing chemical substance of configuration, dilute solution
Dilute solution is packed into case, dilute solution is the tap water or configuration liquid of storage and dechlorination processing 24 hours or more, it is desirable that water
Matter hardness, pH, dissolved oxygen are for the suitable scope of examination biology；
Static experiment method: flow, the first peristaltic pump of stock solution and dilute solution are set by a certain percentage according to experimental concentration
With the second wriggling pump startup, dwell time；In reaction solution case after mixing, operation is entered by third peristaltic pump
Case, static experiment method does not need replacement reaction solution during the experiment, at this point, need to guarantee reaction solution temperature, dissolved oxygen and pH
Deng in optimum range, according to the various concentration of experimental design, the settable multiple groups of the device share stock solution and dilute molten
Liquid is mixed into the reaction solution of various concentration, sets multiple groups device and start and stop by designing different flow velocity and time
Time, can synchronize development experiment, save time and manpower；
Semi-static experimental method: this static experiment method needs (such as 24 hours) replacement primary first-order equation solution at regular intervals, passes through
Set the 4th peristaltic pump, the velocity of rotation of third peristaltic pump and working time realize timing, it is quantitative automatic change liquid function, often
The secondary liquid that changes guarantees that the concentration of reaction solution is not less than the 80% of initial concentration；
Continuous-flow type experimental method: it when carrying out continuous-flow type experiment, needs to exchange water inlet and water outlet, starts the 4th after exchange and wriggle
Pump, the water inlet of continuous bottom and the top of realization device are discharged, and during experiment, can adjust the disengaging flow of reaction solution at any time.
The utility model has the advantages that
Control box is made of organic glass, and dischargeable capacity is determined according to the long size of body for examination biology, it is desirable that meets biology most
Big bearing capacity.Control box built-in fabric net, web can select suitable aperture according to the size of fish, prevent juvenile fish and prelarva
Into water inlet, water outlet.Insulating layer and heater are set outside control box, by temperature sensor measurement water temperature, and by
Temperature controller control heating, it is ensured that guarantee that experimental temperature is constant, control at ± 1 DEG C.Micro-hole aerator is arranged in control box bottom, even
It is connected to aeration pump, dissolved oxygen sensor measures the dissolved oxygen concentration in water body, feeds back to automatic controller, when lower than for examination biology
It is required that dissolved oxygen concentration when, automatic controller control open aeration pump be aerated 10- 20 minutes, to guarantee water in experimental provision
Dissolved oxygen in body is sufficient.It is provided with different sample taps on control box, noxious material in water body can be monitored at any time and routinely refers to
The variation of (pH, COD etc.) is marked, temperature and dissolved oxygen can directly read the result of temperature sensor, dissolved oxygen sensor measurement.By
Each time relay cooperates corresponding peristaltic pump control experimental provision in inflow and outflow in different time periods, spinner flowmeter control
Reaction solution flow of inlet water processed.By control box top sample mouth, it can also periodically take out biological subject sample and carry out toxicity credit
Analysis.Stock solution case, dilute solution case, reaction solution case anabolic reaction solution prepare part, stock solution case and dilute solution
Case is respectively provided with the first peristaltic pump, the second peristaltic pump, and the first peristaltic pump, the second peristaltic pump are respectively arranged with first time relay
Device, second time relay, can timing, quantitative inflow reaction solution case.
Static method, semi-static method and continuous-flow type experimental method kind toxicity test method can be realized by adjusting the application, met
Kinds of experiments demand；The application realize in experiment timing in unmanned situation, it is quantitative automatic change water, reduce the time with manually at
This；Reaction solution can matching while using, solve because at any time extend water body Poisoning material concentration it is unstable caused by interference with
Error, it is ensured that experiment is smoothly carried out；It realizes temperature, dissolved oxygen real-time monitoring and controls accurate, it is ensured that meet for examination biology
Psychological need.Can be used for fish are short-term, outside long term toxicity test, it can also be used to pollutant enrichment experiment in Fish is ground
Study carefully, realizes multifunctional comprehensive experiment.
Detailed description of the invention
Fig. 1 is a kind of integrated experimental device structural schematic diagram for toxicity in fish research of the present invention.
Specific embodiment
The present invention will be further described in detail below based on the drawings and embodiments:
A kind of integrated experimental device for toxicity in fish research, including stock solution case 1, dilute solution case 2, reaction solution
Case 7, temperature controller 12, temperature sensor 14, aeration pump 19, automatic controller 20, water tank 24, the stock solution case 1 pass through
Pipeline connect 3 side of the first peristaltic pump, 3 other side of the first peristaltic pump by pipeline connection reaction solution case 7, described first
First time relay 5 is provided on peristaltic pump 3, the dilute solution case 2 connects 4 side of the second peristaltic pump, institute by pipeline
State 4 other side of the second peristaltic pump and reaction solution case 7 connected by pipeline, be provided on second peristaltic pump 4 second time after
Electric appliance 6,7 right end of reaction solution case are sequentially connected third peristaltic pump 9, spinner flowmeter 10, water inlet 11, institute by pipeline
It states and is provided with the third time relay 8 on third peristaltic pump 9, the 11 right end attended operation case 17 of water inlet, the water inlet
11 are located at 17 upper left quarter of control box, water outlet 21 are connected on the right side of the control box 17, it is right that the water outlet 21 is located at control box 17
Lower part, 21 right end of water outlet connect 22 side of the 4th peristaltic pump by pipeline, and 22 other side of the 4th peristaltic pump passes through
Pipeline connects water tank 24, is provided with the 4th time relay 23 on the 4th peristaltic pump 22, has on the upside of the control box 17
There are multiple sample taps 15, fills reaction solution inside the control box 17.
Insulating layer 13 is connected on the outside of the control box 17, heater is set on the inside of the insulating layer 13, and the heater is logical
Conducting wire connection temperature controller 12 is crossed, the temperature controller 12 connects temperature sensor 14,14 lower end of temperature sensor by conducting wire
In intercalation reaction solution, the setting of temperature sensor 14 is in 17 left side of control box.
The automatic controller 20 connects 19 side of aeration pump, and 19 other side of aeration pump connects micro-hole aerator 16,
The micro-hole aerator 16 is arranged in 17 bottom of control box.
The automatic controller 20 connects dissolved oxygen sensor 18,18 lower end of the dissolved oxygen sensor insertion by conducting wire
In reaction solution, the setting of dissolved oxygen sensor 18 is on 17 right side of control box.
A kind of integrated experimental device operating method for toxicity in fish research:
The following steps are included: stock solution is configuration containing chemical substance step 1: being packed into stock solution in stock solution case 1
Highly concentrated solution, be packed into dilute solution in dilute solution case 2, dilute solution be storage and dechlorination is handled 24 hours or more certainly
Water or configuration liquid, it is desirable that water hardness, pH, dissolved oxygen are for the suitable scope of examination biology；
Step 2: static experiment method: be arranged by a certain percentage according to experimental concentration stock solution and dilute solution flow, first
Peristaltic pump 3 and the starting of the second peristaltic pump 4, dwell time；In reaction solution case 7 after mixing, by third peristaltic pump 9 into
Enter operation case 17.Static experiment method does not need replacement reaction solution during the experiment.At this point, need to guarantee reaction solution temperature
Degree, dissolved oxygen and pH etc. are in optimum range.According to the various concentration of experimental design, the settable multiple groups of the device share deposit
Solution and dilute solution are mixed into the reaction solution of various concentration by designing different flow velocity and time.Set multiple groups dress
The time started and stopped is set, development experiment can be synchronized, save time and manpower；
Semi-static experimental method: this static experiment method needs (such as 24 hours) replacement primary first-order equation solution at regular intervals, passes through
The 4th peristaltic pump 22, the velocity of rotation of third peristaltic pump 9 and working time are set to realize timing, quantitative automatic change liquid function
Can, liquid is changed every time guarantees that the concentration of reaction solution is not less than the 80% of initial concentration；
Continuous-flow type experimental method: it when carrying out continuous-flow type experiment, needs to exchange water inlet 11 and water outlet 21, starts the 4th after exchange
Peristaltic pump 22, the water inlet of continuous bottom and the top of realization device are discharged.During experiment, the disengaging stream of reaction solution can be adjusted at any time
Amount.
Control box 17 is made of organic glass, and dischargeable capacity is determined according to the long size of body for examination biology, it is desirable that meets life
The maximum bearing capacity of object.17 built-in fabric net of control box, web can select suitable aperture according to the size of fish, prevent children
Fish and prelarva enter water inlet 11, water outlet 21.Insulating layer 13 and heater are set outside control box 17, pass through temperature sensor 14
Water temperature is measured, and is controlled and is heated by temperature controller 12, it is ensured that guarantees that experimental temperature is constant, controls at ± 1 DEG C.Control box 17
Micro-hole aerator 16 is arranged in bottom, is connected with aeration pump 19, and dissolved oxygen sensor 18 measures the dissolved oxygen concentration in water body, feedback
To automatic controller 20, when being lower than the dissolved oxygen concentration for trying biological requirement, aeration pump 19 is opened in the control of automatic controller 20
Aeration 10- 20 minutes, to guarantee that the dissolved oxygen in experimental provision in water body is sufficient.Different samplings is provided on control box 17
Mouth 15, can monitor the variation of noxious material and conventional index (pH, COD etc.) in water body, temperature and dissolved oxygen can directly be read at any time
The result for taking temperature sensor 14, dissolved oxygen sensor 18 to measure.Corresponding peristaltic pump control is real with each time relay
Experiment device controls reaction solution flow of inlet water in inflow and outflow in different time periods, spinner flowmeter 10.Pass through control box 17
Top sample mouth 15 can also periodically take out biological subject sample and carry out toxicological analysis.Stock solution case 1, dilute solution case 2,
7 anabolic reaction solution of reaction solution case prepares part, stock solution case 1 and dilute solution case 2 be respectively provided with the first peristaltic pump 3,
Second peristaltic pump 4, the first peristaltic pump 3, the second peristaltic pump 4 are respectively arranged with first time relay 5, second time relay 6,
It can timing, quantitative inflow reaction solution case 7.
3 kinds of static method, semi-static method and continuous-flow type experimental method toxicity test methods can be realized by adjusting the application, met
Kinds of experiments demand；The application realize in experiment timing in unmanned situation, it is quantitative automatic change water, reduce the time with manually at
This；Reaction solution can matching while using, solve because at any time extend water body Poisoning material concentration it is unstable caused by interference with
Error, it is ensured that experiment is smoothly carried out；It realizes temperature, dissolved oxygen real-time monitoring and controls accurate, it is ensured that meet for examination biology
Psychological need.Can be used for fish are short-term, outside long term toxicity test, it can also be used to pollutant enrichment experiment in Fish is ground
Study carefully, realizes multifunctional comprehensive experiment.
Claims (5)
1. a kind of integrated experimental device for toxicity in fish research, it is characterized in that: including stock solution case, dilute solution
Case, reaction solution case, temperature controller, temperature sensor, aeration pump, automatic controller, water tank, the stock solution case pass through pipe
Road connects the first peristaltic pump side, and first peristaltic pump other side connects reaction solution case by pipeline, and described first wriggles
First time relay is provided on pump, the dilute solution case connects the second peristaltic pump side by pipeline, and described second is compacted
The dynamic pump other side connects reaction solution case by pipeline, and second time relay is provided on second peristaltic pump, described anti-
It answers liquor box right end to be sequentially connected third peristaltic pump, spinner flowmeter, water inlet by pipeline, is arranged on the third peristaltic pump
There are the third time relay, the water inlet right end attended operation case, the water inlet is located at control box upper left quarter, the operation
Water outlet is connected on the right side of case, the water outlet is located at control box right lower quadrant, and the water outlet right end is compacted by pipeline connection the 4th
Dynamic pump side, the 4th peristaltic pump other side connects water tank by pipeline, when being provided with the 4th on the 4th peristaltic pump
Between relay, there are multiple sample taps on the upside of the control box, fill reaction solution inside the control box.
2. a kind of integrated experimental device for toxicity in fish research according to claim 1, it is characterized in that: the behaviour
Make to connect insulating layer on the outside of case, heater is set on the inside of the insulating layer, and the heater connects temperature controller by conducting wire, described
Temperature controller connects temperature sensor, in the temperature sensor lower end intercalation reaction solution, the temperature sensor by conducting wire
It is arranged on the left of control box.
3. it is according to claim 2 it is a kind of for toxicity in fish research integrated experimental device, it is characterized in that: it is described from
Movement controller connects aeration pump side, and the aeration pump other side connects micro-hole aerator, and the micro-hole aerator setting is being grasped
Make bottom portion.
4. it is according to claim 3 it is a kind of for toxicity in fish research integrated experimental device, it is characterized in that: it is described from
Movement controller connects dissolved oxygen sensor, in the dissolved oxygen sensor lower end intercalation reaction solution, the dissolution by conducting wire
Lambda sensor is arranged on the right side of control box.
5. a kind of integrated experimental device operating method for toxicity in fish research according to claim 4, feature
It is: the following steps are included: stock solution is configuration containing chemical substance step 1: being packed into stock solution in stock solution case
Highly concentrated solution, is packed into dilute solution in dilute solution case, and dilute solution is storage and dechlorination is handled 24 hours or more originally
Water or configuration liquid, it is desirable that water hardness, pH, dissolved oxygen are for the suitable scope of examination biology；
Step 2: static experiment method: be arranged by a certain percentage according to experimental concentration stock solution and dilute solution flow, first
Peristaltic pump and the second wriggling pump startup, dwell time；In reaction solution case after mixing, entered by third peristaltic pump anti-
Control box is answered, static experiment method does not need replacement reaction solution during the experiment, at this point, need to guarantee reaction solution temperature, dissolution
Oxygen and pH etc. are in optimum range, and according to the various concentration of experimental design, the settable multiple groups of the device share stock solution and dilute
Release solution, by designing different flow velocity and time, be mixed into the reaction solution of various concentration, set the starting of multiple groups device and
The time of stopping can synchronize development experiment, save time and manpower；
Or semi-static experimental method: this static experiment method needs (such as 24 hours) replacement primary first-order equation solution at regular intervals, leads to
Cross setting the 4th peristaltic pump, third peristaltic pump velocity of rotation and the working time come realize periodically, it is quantitative automatic change liquid function,
Liquid is changed every time guarantees that the concentration of reaction solution is not less than the 80% of initial concentration；
Or continuous-flow type experimental method: it when carrying out continuous-flow type experiment, needs to exchange water inlet and water outlet, it is compacted to start the 4th after exchange
Dynamic pump, the water inlet of continuous bottom and the top of realization device are discharged, and during experiment, can adjust the disengaging flow of reaction solution at any time.
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| CN201910514834.7A CN110150186A (en) | 2019-06-14 | 2019-06-14 | A kind of integrated experimental device and operating method for toxicity in fish research |
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| CN201910514834.7A CN110150186A (en) | 2019-06-14 | 2019-06-14 | A kind of integrated experimental device and operating method for toxicity in fish research |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111323553A (en) * | 2020-03-13 | 2020-06-23 | 浙江省海洋水产研究所 | A pond of breeding fish for toxicological experiment |
| RU205629U1 (en) * | 2020-06-26 | 2021-07-23 | Общество с ограниченной ответственностью "Экоперспектива плюс" | Dosing device for conducting toxicological experimental studies in flowing conditions |
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