CN106386637B - Multi-station copepod zooplankton culture device and culture method thereof - Google Patents
Multi-station copepod zooplankton culture device and culture method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/047—Liquid pumps for aquaria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Animal Husbandry (AREA)
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- Marine Sciences & Fisheries (AREA)
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Abstract
The invention relates to a multi-station copepod zooplankton culture device, which comprises a water storage tank and a water feeding pipeline connected with the water storage tank, wherein the water feeding pipeline is connected with at least one incubator, the incubator is provided with a bait box and a collecting pipeline, the incubator is connected with the water storage tank through a water return pipeline, the water storage tank is provided with a water inlet pipeline, a water discharge pipeline, an acidifying machine and a temperature sensor, each pipeline is provided with a valve, and the culture device also comprises a control box. The same water storage tank is combined with a plurality of incubator structural designs, the experimental method of the multi-station incubator in the same water area is realized, the contrast experiment can be carried out on different copepods or different bait feeding modes of the same copepods in the same water area, and the water quality consistency of the contrast experiment is ensured. Meanwhile, the culture efficiency is improved, and the cost of the culture equipment is reduced. The water storage tank realizes the internal circulation and external circulation modes of the culture environment, updates the water body of the whole water area, ensures the culture water quality and ensures the culture effect and the survival rate of copepod zooplankton.
Description
Technical Field
The invention relates to the field of marine microorganism cultivation equipment, particularly belongs to the field of mechanical and electrical integration of marine organism cultivation, and particularly relates to a multi-station copepod zooplankton cultivation device.
Background
Copepod zooplankton is an important component of the marine ecosystem, is a secondary producer in the biological chain, and plays an important role in maintaining the balance and stability of the marine ecosystem. Meanwhile, the fish bait contains rich unsaturated fatty acid, is high-quality bait on the upper level of a food chain, and is suitable for being used as initial feed for fishes, shrimps and the like. Zooplankton is generally small in size, short in life cycle and very easy to be affected by external environment changes, and field collection is far from meeting the requirements. Therefore, the large-scale cultivation of marine zooplankton as bait is always a hot spot in aquaculture.
In order to realize the large-scale production of marine zooplankton, the important thing is to develop the culture equipment suitable for the survival of marine zooplankton. The development of a copepod zooplankton culture system plays an important role in promoting the large-scale cultivation of zooplanktons, but some problems still exist and need to be solved urgently.
Chinese patent CN 1440236A zooplankton culture method and culture device and culture using the method, disclose a culture tank for storing the culture solution containing zooplankton; a solid-liquid separation membrane disposed in the culture tank; a device for removing the water-soluble nutrient substances of the zooplankton's reproduction-inhibiting substances and/or bacteria from the culture broth through the solid-liquid separation membrane, and a device for adding the zooplankton's bait and fresh culture broth to the culture tank. However, the equipment can not realize multi-station simultaneous culture and can not realize circulating filtration of internal culture water.
Chinese patent CN 101243781 a discloses an integral type incubator convenient to remove, contains the culture part and washing part and corresponding pipeline fixed with the support, however, this cultivation still can only realize the cultivation of a batch, and regulation such as temperature, acidity is inconvenient, can't realize automated control.
The unit benefit of the existing culture system is still low, and the collection of zooplankton and eggs thereof is inconvenient, so that the survival rate of the eggs and larvae in the later period is not high. This is relevant with the automatic control precision and the scope of culture system, needs to adopt advanced control method and high accuracy detection technology to carry out more accurate detection and control in the aspects such as the timing of the temperature of culture apparatus, pH value, bait is put in and excrement and urine and ovum are cleared up regularly and are collected, provides reasonable accurate culture environment for zooplankton's growth urgently. The research and development of the zooplankton culture system can greatly promote the large-scale culture of marine zooplankton as bait, so that the zooplankton has wide application prospect in the mariculture industry.
Disclosure of Invention
According to the defects, the invention aims to provide the multi-station copepod zooplankton culture device and the culture method thereof, which realize automatic control.
In order to achieve the purpose, the technical scheme of the invention is as follows: multistation copepoda zooplankton culture apparatus, it is including the storage water tank that is used for storing the sea water and the water supply pipeline that links to each other with the storage water tank, water supply pipeline links to each other with at least one incubator that is used for cultivateing copepoda zooplankton, the incubator is equipped with the bait case that is used for storing and throwing in bait, the incubator still is equipped with the collection pipeline that is used for collecting ovum and larva, the incubator passes through the return water pipeline and links to each other with the storage water tank, the storage water tank is equipped with water intake pipe and water drainage pipe, the storage water tank still is equipped with acidizing machine and temperature sensor, water supply pipeline, the collection pipeline, the return water pipeline, water intake pipe and water drainage pipe are equipped with the valve, culture apparatus still includes and the acidizing machine, the valve on temperature sensor and all pipelines links to each other and is used for controlling the acidizing machine, temperature sensor and the control box of all valves.
It is preferable that: also comprises a bracket for placing the culture device.
It is preferable that: the lower end of the incubator is conical.
It is preferable that: the connection part of the conical part at the lower end of the incubator and the upper end is provided with a filter screen.
It is preferable that: the liquid level of the bait box is higher than that of the incubator.
It is preferable that: the water inlet pipeline is provided with a water cooler.
It is preferable that: the water supply pipeline is connected with a submersible pump at the bottom of the water storage tank.
The invention also provides a method for culturing copepod zooplankton, which comprises the following steps,
(1) water inflow: let in through the inlet tube in to the water storage tank and handle the sea water to suitable temperature through the cold water machine to let in the incubator through immersible pump and last water pipe way, in the sea water flows into the storage tank through the return water pipeline when the incubator water level reachs the regulation height, forms interior hydrologic cycle:
(2) culturing: putting the eggs or the larvae of the copepods zooplankton to the lower end of the incubator, placing a filter screen, and regularly putting baits through the control box for culturing;
(3) adjusting: in the culture process, the temperature and the pH value are detected through an acidification machine and a temperature sensor, and are controlled through a control box to keep the set water temperature and the set pH value;
(4) water changing: food residues and water-soluble ammonia at the bottom of the water storage tank are discharged through a drainage pipeline, and simultaneously water is fed and supplemented through a water inlet pipeline to form external water circulation;
(5) egg collection: when one period of culture is finished, the control box controls the collection pipeline to be opened for collection; separating adult and egg or larva of copepod zooplankton, and culturing the egg or larva again.
The invention has the beneficial effects that:
1. and designing a multi-station incubator. The same water storage tank is combined with a plurality of incubator structural designs, the experimental method of the multi-station incubator in the same water area is realized, the contrast experiment can be carried out on different copepods or different bait feeding modes of the same copepods in the same water area, and the water quality consistency of the contrast experiment is ensured. Meanwhile, the culture efficiency is improved, and the cost of the culture equipment is reduced.
2. An internal and external water dual-circulation control system. The water storage tank is connected with a plurality of culture boxes through a water feeding pipeline with a check valve, the submersible pump guides seawater in the water storage tank into the culture boxes respectively, and overflowed water of each culture box flows back to the water storage tank through a return pipe to realize internal circulation of a culture environment. Meanwhile, the water storage tank can periodically start an external circulation mode, so that the water in the whole water area is updated, the culture water quality is ensured, and the culture effect and the survival rate of copepod zooplankton are ensured.
3. And (5) monitoring and accurately controlling parameters in real time. This culture system sets up parameters such as temperature, pH value, circulation and bait input time and carries out real-time supervision, compares data, generates corresponding regulation decision-making, regulates and control the cultivation environment through pH valve regulating unit and temperature regulation unit, makes it keep being favorable to zooplankton production within range of developing. The monitoring and adjusting system has high precision and quick response, and can realize real-time adjustment on the culture environment.
4. Eggs were collected automatically. The egg collecting system is automatically started at regular time, so that the survival rate of the eggs or the larvae is greatly improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic diagram of the apparatus of the present invention.
In the figure: 1-a water storage tank; 2-a scaffold; 3-a collection pipeline; 4-a valve; 5-a bait box; 6-an incubator; 7-a water return pipeline; 8-a filter screen; 9-an acidifying machine; 10-a control box; 11-a water inlet pipeline; 12-a water chiller; 13-a drain line; 14-a temperature sensor; 15-water supply pipeline; 16-submersible pump.
Detailed Description
The present invention is further illustrated by the following specific examples.
As shown in figure 1, the multi-station copepoda zooplankton culture device comprises a water storage tank 1 for storing seawater and a water feeding pipeline 15 connected with the water storage tank 1, wherein the water feeding pipeline 15 is connected with at least one culture tank 6 for culturing copepoda zooplanktons, the culture tank 6 is provided with a bait tank 5 for storing and throwing bait, the culture tank 5 is also provided with a collecting pipeline 3 for collecting eggs and larvae, the culture tank 6 is connected with the water storage tank 1 through a water return pipeline 7, the water storage tank 1 is provided with a water inlet pipeline 11 and a water discharge pipeline 13, the water storage tank 1 is also provided with an acidifying machine 9 and a temperature sensor 14, the water feeding pipeline 15, the collecting pipeline 3, the water return pipeline 7, the water inlet pipeline 11 and the water discharge pipeline 13 are provided with valves 4, the culture device also comprises a control box 10 which is connected with the acidification machine 9, the temperature sensor 14 and all valves 4 on the pipelines and is used for controlling the acidification machine 9, the temperature sensor 14 and all valves 4. Wherein, storage water tank 1, incubator 6, bait case 5 and collection pipeline 3 all adopt food level polytetrafluoroethylene material.
Further, the water supply pipeline 15 may be a general water supply pipeline subdivided into water supply branch pipelines connected to the respective incubators, or a plurality of water supply branch pipelines directly connected to the respective incubators 6. Similarly, the return pipes 7 may be return branch pipes of the respective incubators and then gathered to a total return pipe, or each return branch pipe connected to the incubator 6 may be directly connected to the water storage tank 1. The valves of the water supply line 15 are one-way valves to prevent the plankton and eggs produced by the plankton from flowing back into the reservoir.
Further, all the valves 4 are set as electromagnetic valves, and the opening or closing of the valves is controlled by the control box 10, so that automatic control is realized.
Further, the multi-station copepod zooplankton culture device also comprises a bracket 2 for placing the culture device, so that the position relation among the box bodies is ensured. Meanwhile, the lower end of the support 2 can be provided with wheels, so that the whole culture device can be moved and adjusted conveniently according to the actual environment.
Further, the lower end of the incubator 6 is conical, and the filter screen 8 is arranged at the joint of the conical part of the lower end of the incubator 6 and the upper end, so that the copepod zooplankton and eggs thereof are collected conveniently in a conical area, and the copepod zooplankton is not collected conveniently because the copepod zooplankton is scattered in the whole incubator without isolation. Wherein, filter screen 8 can adopt 80 mesh gauze, and fixed ring that adopts stainless steel material around fixes, and wherein solid fixed ring cooperatees with incubator lower extreme toper part and the shape and the size of upper end junction, through the gravity of stainless steel, avoids filter screen 8 to float in incubator 6, simultaneously, also can fix filter screen 8 in the incubator through fixing device such as other fixed knot, fixed rope. The copepods bait is algae liquid prepared by a plurality of marine microalgae according to a certain proportion, is placed in the bait box 5 and is regularly and quantitatively thrown according to the needs of zooplankton, so that the phenomenon of uneven throwing time and throwing amount caused by manual throwing is avoided.
Furthermore, the liquid level of the bait box 5 is higher than that of the incubator 6, and after the valve body is opened, the bait can automatically flow into the incubator by utilizing gravity, so that the operation is very convenient.
Furthermore, a water cooler 12 is arranged on the water inlet pipeline 11, and the temperature of water entering the water storage tank 1 can be adjusted to a proper temperature through the water cooler 12, so that the copepod zooplankton can grow conveniently.
Further, the water supply pipe 15 is connected with a submersible pump 16 at the bottom of the water storage tank 1, and power for supplying water to the culture tank 6 and water power for external water circulation are provided by the submersible pump 16.
Further, a pH value sensor collects the pH value of the culture solution, the pH value is sent to a CPU through analog-to-digital conversion, the CPU compares the pH value of the culture solution with the pH value (7.2-8.2) required by zooplankton, a reasonable regulator is added into the culture solution, if the pH value of the culture solution is too acid, the pH value regulator is controlled to add alkali liquor, if the pH value of the culture solution is too alkali, the pH value regulator is controlled to add acid liquor, and once the pH value is restored to be within a normal range, the pH value regulator is closed.
Further, the temperature of the culture solution is monitored by using a temperature transmitter with a platinum thermal resistance probe Pt100, a monitoring signal is sent to a CPU through analog-to-digital conversion, and the temperature is controlled according to a temperature set value, so that the culture solution is kept in a reasonable temperature range.
Furthermore, the Hall water flowmeter is used for monitoring water flow, when water passes through the magnetic rotor assembly, the rotor rotates and the rotating speed changes along with the change of the flow, the Hall sensor outputs an electric pulse signal, therefore, the frequency of the electric pulse signal is in direct proportion to the flow of the measured fluid, and the CPU counts the electric pulse signal to calculate the fluid flow.
Further, a real-time clock of the STM32 is configured to be set as the current time, and the timing and timing functions of the system are realized, so that the CPU controls the electromagnetic valve to realize the feeding of the bait every 5h (properly adjusted according to the cultured organisms and the types of the bait) and the collection of the eggs every 3 days (properly adjusted according to the cultured organisms).
Furthermore, the CPU controls the LCD, displays the acquired information such as pH value, temperature, flow and the like on the LCD, displays the real-time, and facilitates experimenters to acquire relevant information in time.
The invention also provides a method for culturing copepod zooplankton, which comprises the following steps,
(1) water inflow: the seawater which is processed to a proper temperature by the water chiller 12 is introduced into the water storage tank 1 through the water inlet pipeline 11 and is introduced into the incubator 6 through the submersible pump 16 and the water feeding pipeline 15, and when the water level of the incubator 6 reaches a specified height, the seawater flows into the water storage tank 1 through the water return pipeline to form internal water circulation.
(2) Culturing: eggs or larvae of copepods zooplankton are thrown to the lower end of the incubator 6, the filter screen 8 is placed, and bait is regularly thrown through the control box 10 for cultivation.
(3) Adjusting: in the culture process, the temperature and the pH value are detected through the acidifying machine 9 and the temperature sensor 14, and are controlled through the control box 10, and the set water temperature and the set pH value are kept; specifically, the acidifying machine 9 monitors and adjusts the PH of the environment in real time, so that the PH of the water is always within an ideal range suitable for normal activity and ingestion of zooplankton. The temperature sensor 14 monitors the water quality temperature in real time and adjusts the water temperature through the water chiller 12.
(4) Water changing: food residue and water-soluble ammonia at the bottom of the water storage tank 1 are discharged through a drainage pipeline 13, and simultaneously water is supplemented through a water inlet pipeline 11 to form external water circulation.
(5) Egg collection: when one period of culture is finished, the control box 10 controls the collection pipeline 3 to be opened for collection; separating adult and egg or larva of copepod zooplankton, and culturing the egg or larva again.
The processes of internal and external water circulation, temperature regulation, pH value regulation, bait feeding and egg collection of the culture device are all completed by the control box, so that the intellectualization and the accuracy of the control process are realized. Can be equipped with the display screen on the control box, can show the environmental condition (temperature, PH value etc.) in real time, can carry out parameter setting as required through the button. The whole system is convenient to operate, accurate in control and capable of effectively guaranteeing the animal culture process, meanwhile, the device adopts a multi-station mode, production efficiency is improved, and conditions are provided for contrast tests in the same environment.
Claims (4)
1. Multistation copepod zooplankton culture apparatus which characterized in that: it includes storage water tank (1) that is used for storing the sea water and last water pipe way (15) that links to each other with storage water tank (1), last water pipe way (15) link to each other with a plurality of incubators (6) that are used for cultivateing copepods zooplankton, the lower extreme of incubators (6) be the toper, the lower extreme toper part and the upper end junction of incubators (6) are equipped with filter screen (8), incubators (6) be equipped with bait case (5) that are used for storing and throwing in bait, the liquid level of bait case (5) be higher than the liquid level of incubators (6), incubators (6) still be equipped with collection pipeline (3) that are used for collecting ovum and larva, incubators (6) link to each other with storage water tank (1) through return water pipeline (7), storage water tank (1) be equipped with inlet channel (11) and drainage pipeline (13), storage water tank (1) still be equipped with acidizing machine (9) and temperature sensor (14), water supply pipe way (15), collection pipeline (3), return water pipeline (7), water intake pipe (11) and drainage pipe way (13) be equipped with valve (4), culture apparatus still include and link to each other with acidizing machine (9), temperature sensor (14) and valve (4) on all pipelines and be used for controlling control box (10) of acidizing machine (9), temperature sensor (14) and all valve (4), all valve (4) are established to the solenoid valve to carry out opening or closing of control valve (4) through control box (10), culture apparatus has realized under the condition of quality of water uniformity to the different baits of the same waters different copepodae or the same kind of copepodae different bait input mode and carry out the contrast experiment.
2. The multi-station copepod zooplankton culture device of claim 1, further comprising: also comprises a bracket (2) for placing the culture device.
3. The multi-station copepod zooplankton culture device of claim 1, further comprising: a water cooler (12) is arranged on the water inlet pipeline (11).
4. The multi-station copepod zooplankton culture device of claim 1, further comprising: the water supply pipeline (15) is connected with a submersible pump (16) at the bottom of the water storage tank.
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| CN106900628B (en) * | 2017-04-07 | 2022-07-08 | 安徽师范大学 | Laboratory zooplankton culture apparatus |
| CN107410119A (en) * | 2017-05-17 | 2017-12-01 | 国家海洋局第二海洋研究所 | Culture apparatus and culture instrument |
| CN108990848A (en) * | 2018-08-16 | 2018-12-14 | 国家海洋局第二海洋研究所 | Automatic water changer and zooplankter culture systems |
| CN113197133A (en) * | 2021-05-13 | 2021-08-03 | 珠江水利委员会珠江水利科学研究院 | A integration culture apparatus for zooplankton hatching breeds |
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| US5188851A (en) * | 1989-11-21 | 1993-02-23 | Sanyo-Kokusaku Pulp Co., Ltd. | Feed composition for fish breeding including mononucleotides |
| CN201821752U (en) * | 2010-09-26 | 2011-05-11 | 攀枝花市银江金勇工贸有限责任公司 | River boat type solar power fish-farming net cage |
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