RU205629U1 - Dosing device for conducting toxicological experimental studies in flowing conditions - Google Patents

Abstract

The utility model relates to the automatic regulation of the fluid flow and can be applied in ecology for the purpose of biotesting the toxicity of wastewater in flowing conditions, aquatic toxicology to determine the chemical properties and toxicity, newly synthesized and previously known pollutants, medicine and pharmacology to study the effects of medicinal and other preparations for microbiological processes. The technical result consists in providing dosing for conducting toxicological experimental studies in flowing conditions. This result is achieved by the fact that the device contains a frame 1, a container for toxicant 2, a container for water 3, peristaltic pumps 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, an adder 14, flow mixers 15, flow dividers 16, adjacent aquariums 17, 18, 19, 20, 21, 22, Y-tubes 23, manifold 24, outlet 25, electric heaters 26, O-rings 27, bubbler 28.2 ill.

Description

The utility model relates to the automatic regulation of the fluid flow and can be applied in ecology for the purpose of biotesting the toxicity of wastewater in flowing conditions, aquatic toxicology to determine the chemical properties and toxicity, newly synthesized and previously known pollutants, medicine and pharmacology to study the effects of medicinal and other preparations for microbiological processes.

The closest to the utility model is an automatic dispenser for adjusting the concentrations of test substances in a liquid (Patent No. 2037180 publ. 09.06.1995), containing Mariotte vessels with test substances, upper dividing vessels with a liquid, a liquid receiving compartment and supply compartments equipped with outlet siphon tubes, lower vessels for mixtures of test substances with liquid, water locks and tees.

Among the disadvantages of this automatic dispenser for regulating the concentration of test substances in a liquid, the main ones are the following:

1) the lack of flexible adjustments for the supply of water and mother liquor;

2) lack of adjustment of the rate of delivery of the finished solution to the test object;

3) the lack of the possibility of preparing solutions not multiples of 1/2 concentrations;

4) mixing of the mother liquor with water is not guaranteed, which, in turn, does not exclude their stratification, since the liquids are drained into the reservoir without any mixing systems;

5) lack of automatic supply of the test solution and water to the vessel with the test object;

6) there is no provision for regulating the rate of production of the solution over a wide range;

7) lack of an organized place for the content of the test object;

8) there is no system for collecting the waste solution runoff for further disposal.

The problem is solved by the fact that the regulated intake, as well as the dosed supply of the liquid concentrate of the toxicant and water, are carried out by peristaltic pumps in pairs, through the adder of two flows, connected in series with the flow mixer of liquid media, where they are thoroughly mixed, ending with a flow divider into two equally-volume freely pouring flow to the tanks for the maintenance of the test object, equipped with a device for automatically maintaining the level of the mixture in the tank to ensure flow, combined into a system of centralized drainage of surplus for further disposal.

New essential features:

1) flexible adjustments of the fluid supply are provided by replacing the uncontrolled fluid movement under the action of gravitational forces stabilized by the Mariotte vessel with the supply by a peristaltic pump driven by a stepper motor;

2) regulation of the rate of supply of the ready-made solution to the test object is provided with the possibility of stepless regulation in a wide range of revolutions of the stepper motor of the drive of the peristaltic pump;

3) the preparation of the solution is carried out by synchronized paired operation of two peristaltic pumps, of which the first supplies the toxicant, and the second supplies pure water, while adjusting the supply volumes in time, incorporated in the designs of the pumps, allows you to flexibly change the ratio of the supplied liquids to the flow mixer;

4) the use of a commercially available in-line mixer after the combiner of the toxicant and water flows guarantees high-quality mixing of both components;

5) a divider installed in series after the in-line mixer into two equal streams, freely pouring into two adjacent tanks, providing for the maintenance of test objects in identical conditions to ensure the conditions for repeating the experiment, closes the automatic system: intake of initial components - mixing - delivery to the tank with the test - object;

6) synchronous proportional change in the volume of supply of toxicant and water in time by pairs of pumps makes it possible to regulate the rate of solution production within the limits up to the maximum pump performance;

7) for the maintenance of test objects, the installation is equipped with 12 flow-through aquariums, providing the maintenance of five experimental groups and one control in two replicates, which fully meets the conditions for conducting toxicological experiments;

8) a system for automatic maintenance of the medium level in aquariums for keeping test objects, represented by overturned Y-shaped tubes, the drain parts of which are united by a collector, which organizes the collection of the released excess medium into a single stream, which can subsequently be directed to any processing, provides conditions for collection for the disposal of solutions.

The listed new essential features are sufficient in all cases covered by the requested scope of legal protection.

The identified distinctive features, together with the known ones, provide a technical result.

FIG. 1 shows a diagram of a dosing device for conducting toxicological experimental studies in flowing conditions; in fig. 2 shows a diagram of an aquarium for keeping a test object with automatic life support systems and maintaining the liquid level.

The dosing device for conducting toxicological experimental studies in flowing conditions contains a frame 1, a container for a toxicant 2, a container for water 3, peristaltic pumps 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, an adder 14, in-line 15 mixers, 16 flow dividers, adjacent aquariums 17, 18, 19, 20, 21, 22, Y-tubes 23, manifold 24, outlet 25, electric heaters 26, O-rings 27, bubbler 28.

The proposed dispensing device operates as follows. Reservoirs 2 and 3 are filled with initial liquids, for example, reservoir 2 is supplied with a toxicant solution at the maximum concentration planned in the study, and reservoir 3 is filled with water, which will be diluted with the concentration of reservoir 2 and will also contain a control group of test objects. The containers are made of inert, chemically resistant materials.

Pumps 4 and 13 are set for maximum performance. Further, depending on the nature of the study, various options for the operation of pumps are possible, solutions can be produced both in the same and in different concentrations. As an example, let us consider the classical biotesting scheme, with the division of the solution concentration by a multiple of two. In this case, pumps 5 and 9 are set to half their capacity, causing a 2-fold decrease in the concentration of the solution, pump 6 is set to 1/4 of the capacity, pump 10 to 3/4, pump 7 to 1/8, pump 11 to 7/8, pumps 8 to 1/16, pump 12 to 15/16 to form volumes to obtain the following toxicant concentrations, respectively: 1, 1/2, 1/4, 1/8, 1/16 and 0. Pumps 5, 6, 7 , 8, 9, 10, 11 and 12 liquids through the corresponding adders 14 are fed to the flow mixers 15, where they are qualitatively mixed to obtain ready-made solutions. Ready solutions, simultaneously with liquids supplied by pumps 4 and 13, through dividers 16 enter pairs of adjacent aquariums 17, 18, 19, 20, 21 and 22 with the same, in pairs, concentrations to comply with the principle of repeatability in the experiment. As the aquariums fill up to 1/3 of the volume, electric heaters 26 are switched on, automatically setting the required water temperature. At the moment when the volumes of the aquariums are filled to 100%, the supply of all pumps synchronously, in the same proportions, is set to the capacity required in terms of flow (it depends on the conditions of the experiment).

To remove excess medium, a Y-shaped tube 23 is installed in the aquarium, one end of which is immersed to 3/4 of its depth and protected by a mesh to prevent the test object from entering it, the second is brought out above the medium level in order to avoid the bellows effect, and the third, through which was overflowed, passed through a hole in the wall of the aquarium, sealed by a leak-free O-ring 27. Thus, automatic maintenance of the level in the aquarium is ensured. In this case, the intake of liquid from the aquarium is carried out from the bottom part, which excludes the accumulation of waste products of the test object at the bottom. Waste from the Y-shaped pipes through the collector 24 enter the outlet 25 and then for disposal.

Then the bubbler 28 is turned on. The installation is ready for experiments.

The proposed installation can successfully work with various options for diluting the concentration of the initial solution, the adjustment is carried out by steplessly changing the rotational speed of the rotors of the stepper motors of the drives operating in a pair of peristaltic pumps. A multiple change in the performance of all pumps simultaneously allows you to regulate the flow rate of aquariums in a wide range, thereby expanding the experimenter's capabilities in the number and quality of test objects.

Claims (1)

A dosing device for conducting toxicological experimental studies in flowing conditions is characterized by the fact that the test solution and water, for keeping the control group of test objects, are supplied by pairs of peristaltic pumps through a flow mixer into pairs of adjacent aquariums for keeping the test object, equipped with life support systems and an automated system maintaining the level with a collector for collecting waste effluents into a single branch pipe.

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