CN215610624U - Gradient mixer - Google Patents
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- CN215610624U CN215610624U CN202122096383.5U CN202122096383U CN215610624U CN 215610624 U CN215610624 U CN 215610624U CN 202122096383 U CN202122096383 U CN 202122096383U CN 215610624 U CN215610624 U CN 215610624U
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Abstract
The utility model discloses a gradient mixer, which comprises a first mixing pump, a second mixing pump and a mixer, wherein the first mixing pump is provided with a first liquid inlet and a first liquid outlet; the second mixing pump is provided with a second liquid inlet and a second liquid outlet; the outlet of the first liquid outlet and the outlet of the second liquid outlet are respectively communicated with the inlet of the mixer, and the outlet of the mixer is connected with a sample adding needle. According to the utility model, the first solution and the second solution are fed by the two mixing pumps, so that mixed samples with any specified quantity can be rapidly, efficiently and flexibly prepared.
Description
Technical Field
The utility model relates to a mixing device, in particular to a gradient mixer.
Background
The mixer is a device for gradually changing the components of the solution in a set manner, and can be used for controlling the change of the solubility or the change of the acidity of a certain component in the solution. When the ion exchange chromatography column is used for separating biomacromolecules, eluent with continuous pH gradient or continuous ionic strength gradient is often required; ultracentrifugation preparation also often requires the production of a continuous density gradient, etc., for separating a sample. The existing gradient mixer utilizes the principle of a communicating vessel to keep the liquid levels of two containers for containing concentrated and diluted solutions in balance, and adjusts the mixing speed and the output flow of the two solutions so as to make linear gradients with different slopes; a magnetic core can be arranged in the output cup body to stir and mix so that gradient change is more uniform, or the magnetic stirrer can be used as a magnetic stirrer. The mixer for realizing gradient change of gel by utilizing the principle of a communicating vessel relies on manual regulation and control of output flow, uniform and continuous change is difficult to realize, and slope liquid of linear gradient is difficult to control; the solution in the two containers must be completely discharged to prepare the gradient gel with a certain concentration or within a certain density range, so that waste is difficult to avoid and the flexibility is poor; the outflow of the solution in the whole process depends on the action of gravity, is relatively slow, and is influenced by the pressure reduction, the flow and the gradient change along with the liquid level reduction.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: in order to solve the problems of the prior art, the utility model provides a gradient mixer, which controls the amount of solution fed into the mixer through a mixing pump to realize high-precision mixing of the solution.
The technical scheme is as follows: the utility model relates to a gradient mixer, which comprises a first mixing pump, a second mixing pump and a mixer, wherein the first mixing pump is provided with a first liquid inlet and a first liquid outlet; the second mixing pump is provided with a second liquid inlet and a second liquid outlet; the outlet of the first liquid outlet and the outlet of the second liquid outlet are respectively communicated with the inlet of the mixer, and the outlet of the mixer is connected with a sample adding needle.
As a preferable structure of the present invention, the first liquid inlet is communicated with a first container containing a first solution, and the second liquid inlet is communicated with a second container containing a second solution.
As a preferable structure of the present invention, the first liquid inlet is communicated with the first container through a first liquid inlet pipe.
As a preferable structure of the present invention, the first liquid outlet is communicated with the mixer through a first liquid outlet pipe.
As a preferable structure of the present invention, the second liquid inlet is communicated with the second container through a second liquid inlet pipe.
As a preferable structure of the present invention, the second liquid outlet is communicated with the mixer through a second liquid outlet pipe.
As a preferable structure of the present invention, the mixer is a hollow cavity communicated with the first liquid outlet and the second liquid outlet.
As a preferable structure of the present invention, the sample addition needle is detachably connected to the outlet of the mixer.
As a preferred structure of the present invention, in the sample adding period, the pumping speed of the first mixing pump decreases from the maximum speed to the minimum speed at a constant speed, and the pumping speed of the second mixing pump increases from the minimum speed to the maximum speed at a constant speed.
In a preferred embodiment of the present invention, the flow rate of the solution fed into the inlet of the mixer is kept constant during the sample application period.
The principle of the gradient mixer of the utility model for realizing gradient change of mixed solution is as follows: the proportion of the solutions with different concentrations injected into the container is adjusted by controlling the flow rate of the mixing pump at different moments, the flow rate of the concentrated solution is large (or small) at the beginning, and the flow rate of the diluted solution is small (or large), so that the concentrated solution in the solution is large (or small), the diluted solution is small (or large), the concentration of the solution is relatively large (or small), the flow rate of the concentrated solution becomes small (or large), the flow rate of the diluted solution becomes small (or large), the concentration of the concentrated solution in the solution is reduced (or increased), the ratio of the diluted solution is increased (or reduced), and the concentration of the solution is relatively small (or large) along with the change of time, so that the concentration gradient is formed.
Has the advantages that: (1) according to the utility model, the first solution and the second solution are fed by the two mixing pumps, so that the mixed samples with any specified quantity, such as gradient gel, can be rapidly, efficiently and flexibly prepared; (2) the utility model realizes the fine regulation and control of the slope of the linear gradient by controlling the solution feeding speed of the two mixing pumps and keeping the flow of the solution at the inlet of the mixer unchanged; (3) the liquid inlet of the mixing pump is communicated with the container through the liquid inlet pipeline, so that the solution can be recycled, the defect that a small amount of samples such as gel are wasted in the prior art is overcome, and the problem that a single sample or a few samples such as gel are difficult to prepare in the prior art is overcome; (4) the utility model realizes gradient mixing by the mixing pump and solves the defect of slow preparation process in the prior art.
Drawings
FIG. 1 is a schematic diagram of the gradient mixer of the present invention;
FIG. 2 is a schematic structural diagram of the gradient mixer of the present invention.
Detailed Description
The structure of the present invention will be further explained with reference to the accompanying drawings.
The utility model provides a gradient mixer, which comprises a first mixing pump 1, a second mixing pump 2 and a mixer 3. As shown in fig. 1, the first mixing pump 1 and the second mixing pump 2 are both fixed on the housing of the gradient mixer, and the first mixing pump 1 and the second mixing pump 2 in this embodiment are both peristaltic pumps. The first mixing pump 1 is provided with a first liquid inlet 101 and a first liquid outlet 102; in this embodiment, the first liquid inlet 101 is connected to the first container 5 containing the first solution through a first liquid inlet pipe 103, and the first liquid outlet 102 is connected to the mixer 3 through a first liquid outlet pipe 104. The second mixing pump 2 is provided with a second liquid inlet 201 and a second liquid outlet 202, the second liquid inlet 201 is communicated with a second container 6 containing a second solution through a second liquid inlet pipeline 203, and the second liquid outlet 202 is communicated with the mixer 3 through a second liquid outlet pipeline 204. In this embodiment, the first liquid inlet pipeline 103, the first liquid outlet pipeline 104, the second liquid inlet pipeline 203 and the second liquid outlet pipeline 204 are corrosion-resistant PVC hoses. The first liquid inlet pipeline 103 is detachably connected with the first liquid inlet 101, the second liquid inlet pipeline 203 is detachably connected with the second liquid inlet 201, when different mixed solutions need to be replaced, pipelines communicated with the first liquid inlet 101 and the second liquid inlet 201 can be detached at any time, so that the replacement of the solutions is realized, meanwhile, liquid can be fed through the mixing pump, the liquid feeding precision and the volume of the mixed solutions can be controlled at high precision, the preparation of small-volume samples is realized, and the waste of the solutions is avoided.
In this embodiment, the first solution and the second solution are determined according to the prepared target sample, such as elution solutions with different concentrations, acid and alkali solutions with different pH, or solutions for preparing gradient gel, etc. The first container 5 and the second container 6 of the present invention may be a reagent bottle.
The exit linkage of blender 3 has application of sample needle 4, and application of sample needle 4 can be dismantled with the export of blender 3 and be connected, can select the application of sample needle of different specifications according to actual need.
In the present embodiment, the mixer 3 is a hollow cavity communicated with the first liquid outlet 102 and the second liquid outlet 202, as shown in fig. 2, the mixer 3 is a hollow cavity having an inner diameter larger than the sum of the first liquid outlet 104 and the second liquid outlet 204, so that the first solution and the second solution can be fully mixed in the mixer 3, and the fully mixed solution is sent out from the sample injection needle 4.
In the sample adding period of the gradient mixer, the pump liquid speed of the first mixing pump 1 is reduced from the maximum speed to the minimum speed at a constant speed, the pump liquid speed of the second mixing pump 2 is increased from the minimum speed to the maximum speed at a constant speed, and the flow of the solution sent from the inlet of the mixer 3 is kept unchanged in the sample adding period, so that an instrument for quickly, efficiently and flexibly preparing gradient gel with any specified quantity can be realized, and the defects of the gradient mixer in the prior art are overcome.
The working method for preparing the gradient gel of the gradient mixer comprises the following steps: the method comprises the steps that a concentrated gel solution for preparing gel is contained in a first container 5, a diluted gel solution for preparing gel is contained in a second container 6, the gel with concentration gradient change is obtained by finely controlling the instantaneous flow rates of the concentrated gel solution and the diluted gel solution, namely, the first mixing pump 1 and the second mixing pump 2 respectively inject the gel into a mixer 3 at variable speed, the concentrated gel solution is injected into the first mixing pump 1, the instantaneous flow rate is from large to small (or from small to large), the diluted gel solution is injected into the second mixing pump 2, the instantaneous flow rate is from small to large (or from large to small), and the solutions flowing out of the two mixing pumps are fully mixed in advance through the mixer 3 and then are injected into a designated container through a pipeline; the slope of the linear gradient curve can be adjusted by simultaneously controlling the sum of the instantaneous flow rates of the two mixing pumps at the same moment, and the total volume of the solution injected into the container in a certain time is equal to the sum of the integral of the instantaneous flow rates of the two mixing pumps over time.
Claims (10)
1. A gradient mixer is characterized by comprising a first mixing pump (1), a second mixing pump (2) and a mixer (3), wherein the first mixing pump (1) is provided with a first liquid inlet (101) and a first liquid outlet (102); the second mixing pump (2) is provided with a second liquid inlet (201) and a second liquid outlet (202); the outlet of the first liquid outlet (102) and the outlet of the second liquid outlet (202) are respectively communicated with the inlet of the mixer (3), and the outlet of the mixer (3) is connected with a sample adding needle (4).
2. Gradient mixer according to claim 1, wherein the first inlet (101) is in communication with a first container (5) containing a first solution and the second inlet (201) is in communication with a second container (6) containing a second solution.
3. Gradient mixer according to claim 2, wherein the first liquid inlet (101) communicates with the first container (5) via a first liquid inlet line (103).
4. Gradient mixer according to claim 3, wherein the first liquid outlet (102) communicates with the mixer (3) via a first liquid outlet line (104).
5. Gradient mixer according to claim 4, wherein the second liquid inlet (201) communicates with the second container (6) via a second liquid inlet line (203).
6. Gradient mixer according to claim 5, wherein the second liquid outlet (202) communicates with the mixer (3) via a second liquid outlet line (204).
7. Gradient mixer according to claim 6, wherein the mixer (3) is a hollow cavity communicating with the first liquid outlet (102) and the second liquid outlet (202).
8. Gradient mixer according to claim 7, wherein the sample application needle (4) is detachably connected to the outlet of the mixer (3).
9. Gradient mixer according to claim 8, wherein the pumping speed of the first mixing pump (1) decreases from a maximum speed to a minimum speed at a constant speed and the pumping speed of the second mixing pump (2) increases from a minimum speed to a maximum speed at a constant speed during a sample application period.
10. Gradient mixer according to claim 9, wherein the flow rate of the solution fed in at the inlet of the mixer (3) is maintained constant during the sample application period.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122096383.5U CN215610624U (en) | 2021-09-01 | 2021-09-01 | Gradient mixer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122096383.5U CN215610624U (en) | 2021-09-01 | 2021-09-01 | Gradient mixer |
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| CN215610624U true CN215610624U (en) | 2022-01-25 |
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| CN202122096383.5U Active CN215610624U (en) | 2021-09-01 | 2021-09-01 | Gradient mixer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114836412A (en) * | 2022-05-20 | 2022-08-02 | 广东医科大学 | Linear density gradient solution and preparation method thereof |
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2021
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114836412A (en) * | 2022-05-20 | 2022-08-02 | 广东医科大学 | Linear density gradient solution and preparation method thereof |
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