CN105301211A - Semi-static temperature-control type fish toxicity test apparatus - Google Patents
Semi-static temperature-control type fish toxicity test apparatus Download PDFInfo
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- CN105301211A CN105301211A CN201410317945.6A CN201410317945A CN105301211A CN 105301211 A CN105301211 A CN 105301211A CN 201410317945 A CN201410317945 A CN 201410317945A CN 105301211 A CN105301211 A CN 105301211A
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Abstract
The invention discloses a semi-static temperature-control type fish toxicity test apparatus. The apparatus comprises a semi-static control unit, a toxicity test unit, a temperature-control heating unit, an aeration oxygenation unit and an illuminating unit, wherein test time starts accumulating after the test fish is monitored to be put into fish tanks; if the test time is smaller than the test time threshold, water with preset volume in the fish tanks is replaced according to the monitoring period and informing the toxicity test unit; if the test time is equal to the test time threshold, the test is ended; the fish tank group number and the fish tanks required in each group are configured according to the semi-static temperature-control type fish toxicity test requirements; fish tank test water is prepared according to the test water volume; the test water is dosed in the fish tanks; the test fish is put into the fish tanks; the temperature of the test water is controlled within the set temperature range; oxygenation and aeration are conducted on the fish tanks; illuminating adjustment is conducted on the toxicity test unit through an illuminating device according to the fish toxicity test standards. By adopting the semi-static temperature-control type fish toxicity test apparatus, constant temperature of the water environment and batch operations of the test are achieved.
Description
Technical field
The present invention relates to water environment ecological technique, particularly the semi-static temperature controlled toxicity test device of fishes of one.
Background technology
Water is the basis that the mankind depend on for existence, the health of quality of water environment situation and the mankind and develop closely bound up.Along with socioeconomic fast development, the whole world is also increasing to the demand of metal, makes the exploitation of metal mineral reserves and smelting present the trend of geometric growth.And the heavy metal used in a large number, enter again water environment by all means, cause the pollution of water environment ecology, cause water environment to go from bad to worse, serious threat is caused to ecological security of water environment and human health.Such as, at present, a large amount of industrial wastes and sewage are just drained in river and lake without effective blowdown process, not only cause serious threat to the hydrobiont in river and lake, also threaten the safety in downstream potable water source.
Toxicity test of fishes, as the technological means of Efficient Evaluation quality of water environment, is widely used in the water quality detection in river and lake.That is, toxicity test of fishes utilizes fish as subjects, toxicity research is carried out to pollutant in the water body of water environment, object is to inquire into and illustrates pollutant to the impact of fish and the mechanism of action, thus prevent pollutant in water environment by food harm humans, provide foundation for working out the hygienic standard of pollutant in water body.But at present, for the device of toxicity test of fishes, manipulation is comparatively complicated, cannot maintain the comparatively stationary temperature required in water environment, and, can not batched operation be carried out.
Summary of the invention
In view of this, fundamental purpose of the present invention is to propose a kind of semi-static temperature controlled toxicity test device of fishes, is provided for the device of the semi-static toxicity test of fishes of indoor adjustable temperature, realizes the batched operation of the constant and test of temperature in water environment.
For achieving the above object, the invention provides a kind of semi-static temperature controlled toxicity test device of fishes, this device comprises: semi-static control module, toxicity test unit, heated for controlling temperature unit, aeration aerating unit and light illuminating unit, wherein,
Semi-static control module, for after monitoring and putting into tested fish in the fish jar after dosing, accumulative test period, if accumulative test period is less than the test period threshold value pre-set, according to the monitoring periods pre-set, change the water yield of predetermined volume in fish jar, test water in notice toxicity test unit preparation fish jar, if accumulative test period equals the test period threshold value pre-set, terminate test;
Toxicity test unit, for according to semi-static Temperature Control Type toxicity test of fishes requirement, the fish jar group number needed for pre-configured test and often organize required fish jar number; Water is tested according in the test water capacity preparation fish jar pre-set; According to semi-static Temperature Control Type toxicity test of fishes requirement, dosing is carried out to the test water in fish jar; Put into tested fish in fish jar after dosing, carry out semi-static toxicity test of fishes;
Heated for controlling temperature unit, for according to the test water temperature of testing in the fish jar that obtains, controls test water temperature in fish jar in the temperature range pre-set;
Aeration aerating unit, carries out oxygenation and aeration for testing water in the fish jar to configuration;
Light illuminating unit, for according to toxicity test of fishes standard, carries out Illumination adjusting by candling to toxicity test unit.
Preferably, described toxicity test unit comprises: fish jar, doser, peristaltic pump, framework and flow control mouth, wherein,
The quantity of fish jar is multiple, on the first dividing plate that placement is arranged in the frame, bottom fish jar, offers for controlling the flow control mouth that in fish jar, current are come in and gone out;
The quantity of doser is multiple, and on the second partition that placement is arranged in the frame, described second partition is positioned at above described first dividing plate, and each fish jar is to there being a doser, and doser is connected with fish jar by peristaltic pump;
Preferably, described heated for controlling temperature unit comprises: well heater, electric control box and temperature sensor, wherein,
Described well heater is placed in below described first dividing plate or side;
Temperature sensor is arranged in fish jar;
Electric control box, connects well heater and temperature sensor respectively by electrical conduits.
Preferably, described electric control box comprises: temperature-sensitive sticker, mapping relations table storer, water temperature processor, switch and power device, wherein,
Temperature-sensitive sticker, for receiving the information of temperature sensor transmission, is converted to temperature information by the information of reception, exports the temperature sensor mark of temperature information and the information of reception to water temperature processor;
Mapping relations table storer, for arranging and the mapping relations table that in memory storage, each temperature sensor mark identifies with respective heater;
Water temperature processor, for the temperature information according to reception, determine that temperature is lower than the first temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, turn-on power device identifies the switch between corresponding well heater with the well heater of mapping; Determine that temperature is lower than the second temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, deenergization device identifies the switch between corresponding well heater with the well heater of mapping.
Preferably, described well heater is arranged in the temperature control cylinder groove of framework, and fish jar inwall arranges one for the containing tube of accommodating temperature sensor, described containing tube is lower than the water surface in fish jar, or a described containing tube part immerses the water surface in fish jar, and offers through hole in the part immersing the water surface in fish jar.
Preferably, described aeration aerating unit comprises: oxygenation aerator, and described oxygenation aerator is communicated in fish jar by air shooter; Described light illuminating unit comprises: candling, and described candling is arranged at the top of framework.
Preferably, described device comprises further:
Magnetic stirring apparatus, described magnetic stirring apparatus is along fish jar placement direction, and slide downward enters in fish jar, or upward sliding, shifts out in fish jar.
Preferably, described fish jar, in the total measurement (volume) position of the aqueous solution injected, is provided with the first panel that is laid with sensor, in the total measurement (volume) half position of the aqueous solution injected, is provided with the second panel that another is laid with sensor.
Preferably, described device comprises further:
Float, be arranged between the first panel and the second panel, after described float and the second plate contact, trigger the sensor arranged in the second panel and export to semi-static control module the steering order that adds water, after described float and the first plate contact, trigger the sensor arranged in the first panel and export to semi-static control module the open command that adds water.
Preferably, described semi-static control module comprises: monitor, timer, processor and water-feed, wherein,
Monitor, for after monitoring and putting into tested fish in the fish jar after dosing, sends initiate notification to timer;
Timer, for after receiving initiate notification, starts timing, accumulative test period; After receiving off-test notice, carry out clearing process;
Processor, for according to the monitoring periods pre-set, read the timing in timer, judge whether accumulative test period is less than the test period threshold value pre-set, if so, test water in notice toxicity test unit preparation fish jar, and after monitoring the test water capacity in toxicity test unit fish jar and reducing predetermined volume, start water-feed, after monitoring water-feed inject the aqueous solution of predetermined volume in fish jar, turn off water-feed;
Water-feed, is provided with the water supply hose for supplying water to fish jar, deeply in fish jar.
As seen from the above technical solutions, the semi-static temperature controlled toxicity test device of fishes of one provided by the invention, by being provided for the device of the semi-static toxicity test of fishes of indoor adjustable temperature, water temperature control can being carried out, dissolved oxygen DO controls, and lower water temperature is remained to stationary temperature; By arranging electric control box, all controls of this semi-static temperature controlled toxicity test device of fishes being concentrated in electric control box, intelligentized temperature can be realized and control, manipulating comparatively simple; Further, organize fish jar by arranging more, and arrange and often organize fish jar and be in equivalent environment, different group fish jar can arrange different experiments parameter, thus realizes the batched operation of the constant and test of temperature in water environment.
Accompanying drawing explanation
Fig. 1 is the main TV structure schematic diagram of the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention.
Fig. 2 is the embodiment of the present invention semi-static temperature controlled toxicity test device of fishes plan structure schematic diagram.
Fig. 3 is the semi-static Temperature Control Type toxicity test of fishes method flow schematic diagram based on the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly, the present invention is described in further detail below in conjunction with the accompanying drawings and the specific embodiments.
In prior art, owing to also not having semi-static temperature controlled toxicity test device of fishes, toxicity test of fishes is caused to manipulate complexity, cannot steady temperature be maintained, and, can not batched operation be carried out.
In the embodiment of the present invention, provide a kind of semi-static temperature controlled toxicity test device of fishes, can be used for the water environment ecological toxicology test of pollutant.Many group fish jars are set and carry out batched operation and to arrange the environmental baseline often organizing fish jar identical or substantially identical, and the temperature of water environment is controlled, by carrying out the parallel experiment of many concentration group, thus the impact of different variable can be got rid of, make experimental result more credible, and effectively can alleviate workload, may be used for Multi-example test and parallel check experiment.
In the embodiment of the present invention, semi-static toxicity test of fishes, according to the monitoring periods pre-set, changes water at each monitoring periods, changes the toxicity test of fishes of the water yield of predetermined volume in fish jar.
Fig. 1 is the main TV structure schematic diagram of the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention.
Fig. 2 is the embodiment of the present invention semi-static temperature controlled toxicity test device of fishes plan structure schematic diagram.
See Fig. 1 and Fig. 2, this semi-static temperature controlled toxicity test device of fishes comprises: semi-static control module, toxicity test unit, heated for controlling temperature unit, aeration aerating unit and light illuminating unit (not shown), wherein, semi-static control module is connected with toxicity test unit, and toxicity test unit is also connected with heated for controlling temperature unit, aeration aerating unit and light illuminating unit respectively;
Semi-static control module, for after monitoring and putting into tested fish in the fish jar after dosing, accumulative test period, if accumulative test period is less than the test period threshold value pre-set, according to the monitoring periods pre-set, change the water yield of predetermined volume in fish jar, test water in notice toxicity test unit preparation fish jar, if accumulative test period equals the test period threshold value pre-set, terminate test;
Toxicity test unit, for according to semi-static Temperature Control Type toxicity test of fishes requirement, the fish jar group number needed for pre-configured test and often organize required fish jar number; Water is tested according in the test water capacity preparation fish jar pre-set; According to semi-static Temperature Control Type toxicity test of fishes requirement, dosing is carried out to the test water in fish jar; Put into tested fish in fish jar after dosing, carry out semi-static toxicity test of fishes;
Heated for controlling temperature unit, for according to the test water temperature of testing in the fish jar that obtains, controls test water temperature in fish jar in the temperature range pre-set;
Aeration aerating unit, carries out oxygenation and aeration for testing water in the fish jar to configuration;
Light illuminating unit, for according to toxicity test of fishes standard, carries out Illumination adjusting by candling to toxicity test unit.
In the embodiment of the present invention, semi-static control module comprises: monitor, timer, processor and water-feed (not shown), and wherein, monitor is connected with timer, timer is connected with processor again, and processor is connected with water-feed again;
Monitor, for after monitoring and putting into tested fish in the fish jar after dosing, sends initiate notification to timer;
Timer, for after receiving initiate notification, starts timing, accumulative test period; After receiving off-test notice, carry out clearing process;
Processor, for according to the monitoring periods pre-set, read the timing in timer, judge whether accumulative test period is less than the test period threshold value pre-set, if so, test water in notice toxicity test unit preparation fish jar, and after monitoring the test water capacity in toxicity test unit fish jar and reducing predetermined volume, start water-feed, after monitoring water-feed inject the aqueous solution of predetermined volume in fish jar, turn off water-feed;
Water-feed, is provided with the water supply hose for supplying water to fish jar, deeply in fish jar.
In the embodiment of the present invention, by arranging the float being used for aqueous solution and regulating in fish jar, whether the test water capacity in monitoring fish jar reduces predetermined volume, and, whether inject the aqueous solution of predetermined volume in fish jar.
Toxicity test unit comprises: fish jar 1, doser 2, peristaltic pump 3, framework 4 and flow control mouth 10;
Heated for controlling temperature unit comprises: well heater 5, electric control box 7 and temperature sensor 11;
Aeration aerating unit comprises: oxygenation aerator 6;
Light illuminating unit comprises: candling 9;
Like this, toxicity test unit in semi-static temperature controlled toxicity test device of fishes, heated for controlling temperature unit, aeration aerating unit and light illuminating unit comprise: fish jar 1, doser 2, peristaltic pump 3, framework 4, well heater 5, oxygenation aerator 6, electric control box 7, candling 9, flow control mouth 10 and temperature sensor 11, wherein
The quantity of fish jar 1 is multiple, and be placed on the first dividing plate of setting in framework 4, below the first dividing plate or side, is provided with well heater 5, bottom fish jar 1, offers for controlling the flow control mouth 10 that in fish jar 1, current are come in and gone out;
In the embodiment of the present invention, fish jar 1 is open-top receptacle, such as, can be uncovered drum or uncovered domed vessel, and the first dividing plate can be arranged in the top of framework 4, middle part and bottom.In practical application, well heater 5 can be positioned in the temperature control cylinder groove bottom framework 4.
In the embodiment of the present invention, flow control mouth 10 place offered bottom fish jar 1, is provided with for controlling the flow control valve, throttling valve etc. that in fish jar, aqueous solution flows out.
Preferably, in the total measurement (volume) position of the aqueous solution that fish jar injects, the first panel that is laid with sensor is set, in the total measurement (volume) half position of the aqueous solution that fish jar injects, the second panel that another is laid with sensor is set.Like this, this device can further include:
Float, be arranged between the first panel and the second panel, after described float and the second plate contact, trigger the sensor arranged in the second panel and export to semi-static control module the steering order that adds water, after described float and the first plate contact, trigger the sensor arranged in the first panel and export to semi-static control module the open command that adds water.
In the embodiment of the present invention, semi-static control module adds water to fish jar, after the float that adds water contacts the first panel, stops adding water to fish jar after receiving the steering order that adds water.
Preferably, well heater 5 is for heating for fish jar 1, and the quantity of well heater 5 is one or more, and well heater 5 can be heating tube or resistance wire etc.For the situation that well heater 5 quantity is, in order to ensure the homogeneity of the fish jar heating of top, the heating tube of long strip type can be set to, above the heating tube of long strip type, be placed with multiple fish jar 1 at equal intervals.As embodiment, well heater 5 is arranged in parallel, and often the spacing of row is even, and the spacing of the well heater 5 on each row is even.Certainly, in practical application, for the situation of the corresponding well heater of each fish jar, fish jar also can be set to uneven arrangement, immediately below each fish jar, arranges a well heater.
In practical application, also well heater 5 can be arranged on the side of fish jar, such as, the heating tube of long strip type can be fixed on the same side of row's fish jar.
As embodiment, in order to the security of the heating tube or resistance wire that ensure long strip type, the well heater 5 be placed in below fish jar 1 can be placed in temperature control cylinder groove.
In the embodiment of the present invention, preferably, the general power of well heater 5 is set to 5 ~ 10KW, heating, turning off well heater 5 and stopping heating, to regulate the water temperature in fish jar 1 by starting well heater 5.As embodiment, the water temperature in fish jar 1 controls at 40 DEG C (+0.1 ~ 0.3 DEG C).
The quantity of doser 2 is multiple, and be placed on the second partition of setting in framework 4, described second partition is positioned at above described first dividing plate, and each fish jar 1 is to there being a doser 2, and doser 2 is connected with fish jar 1 by peristaltic pump 3;
Temperature sensor 11 is arranged in fish jar 1;
In the embodiment of the present invention, as embodiment, one can be arranged for the containing tube of accommodating temperature sensor 11 at fish jar 1 inwall, described containing tube is lower than the water surface in fish jar 1, or a described containing tube part immerses the water surface in fish jar 1, and offers through hole in the part immersing the water surface in fish jar 1.Like this, the temperature sensor 11 being positioned over containing tube can measure the water temperature in fish jar 1.
As another embodiment, temperature sensor 11 also can be fixed on temperature control cylinder trench bottom.
Electric control box 7, connects well heater 5 and temperature sensor 11 respectively by electrical conduits;
In the embodiment of the present invention, electrical conduits can be cable.For the situation of the corresponding well heater of each fish jar 1, a temperature sensor 11 is provided with in each fish jar 1, the mapping relations table of temperature sensor and well heater is previously provided with in electric control box 7, according to the temperature of temperature sensor senses, determine that the temperature sensed is lower than the first temperature threshold pre-set, such as, 40.1 DEG C, query mappings relation table, the well heater that the temperature sensor obtaining sensing temperature maps, the electrical conduits that conducting is connected with the well heater of mapping, makes corresponding well heater electrified regulation, heats up to the fish jar above well heater; If determine that the temperature sensed is lower than the second temperature threshold pre-set, such as, 40.3 DEG C, query mappings relation table, the well heater that the temperature sensor obtaining sensing temperature maps, turn off the electrical conduits be connected with the well heater mapped, make corresponding well heater power-off, heating is stopped to the fish jar above well heater.
Preferably, electric control box 7 comprises: temperature-sensitive sticker, mapping relations table storer, water temperature processor, switch and power device (not shown), wherein,
Temperature-sensitive sticker, for receiving the information of temperature sensor transmission, is converted to temperature information by the information of reception, exports the temperature sensor mark of temperature information and the information of reception to water temperature processor;
In the embodiment of the present invention, each temperature sensor is provided with temperature sensor mark.The information of temperature sensor transmission is a simulating signal, and temperature-sensitive sticker carries out analog to digital conversion to simulating signal, obtains digitized temperature information.Carrying out analog to digital conversion about temperature-sensitive sticker to simulating signal is known technology, omits detailed description at this.
Mapping relations table storer, for arranging and the mapping relations table that in memory storage, each temperature sensor mark identifies with respective heater;
Water temperature processor, for the temperature information according to reception, determine that temperature is lower than the first temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, turn-on power device identifies the switch between corresponding well heater with the well heater of mapping; Determine that temperature is lower than the second temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, deenergization device identifies the switch between corresponding well heater with the well heater of mapping.
In the embodiment of the present invention, after actuating switch, power device, by switch and electrical conduits, to heating installation power supply, makes corresponding well heater electrified regulation, heats up to the fish jar above well heater.
Oxygenation aerator 6 is communicated in fish jar 1 by air shooter;
In the embodiment of the present invention, fish jar 1 is furnished with the water reoxygenation oxygenation aerator 6 mated with test water capacity, for the tested fish in fish jar provide enough oxygen.As embodiment, oxygenation aerator 6 can be placed in the same layer of framework 4 with fish jar 1, also can be positioned at other layer of framework 4.
In the embodiment of the present invention, as embodiment, oxygenation aerator 6 is provided with multiple air shooter, is communicated to respectively in corresponding fish jar, makes each fish jar be connected with the oxygen of same airflow speed.Certainly, in practical application, also can configure an oxygenation aerator 6 for each fish jar, the operational factor of the oxygenation aerator 6 of same test group is identical.
Candling 9 is arranged at the top of framework 4.
In the embodiment of the present invention, fish jar 1, dosing peristaltic pump 3 and doser 2 are all placed in framework 4, and described framework 4 is provided with candling 9, wherein, candling 9 can be daylight lamp, incandescent lamp, flash bulb etc., and illumination controls as 100-20000 lux (LX).By regulating illumination intensity, can be used for water environment and simulate in the light conditions of Various Seasonal and different time.
In the embodiment of the present invention, in the temperature control cylinder groove that the fish jar of identical test group is positioned over same temperature and same light according in strength environment, for test provides accurately consistent test condition and experimental enviroment.
In the embodiment of the present invention, as embodiment, this device can further include:
Dosing peristaltic pump 3, the quantity of dosing peristaltic pump 3 is multiple, and be placed on the 3rd dividing plate of setting in framework 4, each doser 2 is to there being a dosing peristaltic pump 3, and doser 2 is by pipeline and dosing peristaltic pump 3, and dosing peristaltic pump 3 is connected with fish jar 1 by pipeline;
In the embodiment of the present invention, dosing peristaltic pump 3 is for regulating the dosing flow of toxicity test of fishes.
As embodiment, described 3rd dividing plate is positioned at above described second partition, and fish jar 1 is connected with doser 2 with pipeline by dosing peristaltic pump 3, and fish jar 1, doser 2 and dosing peristaltic pump 3 are placed in framework 4.
As another embodiment, this device can further include:
Magnetic stirring apparatus 8, magnetic stirring apparatus 8 is along fish jar 1 placement direction, and slide downward enters in fish jar 1, or upward sliding, shifts out in fish jar 1;
In the embodiment of the present invention, consider and need to carry out the operation such as dosing, flushing frequently by doser 2, for the ease of feeding operations, doser can be positioned over (top operational is inconvenient) in the middle part of device.Meanwhile, in order to effectively utilize space, reduce the floor area of device, fish jar 1 also can not adopt the above-mentioned mode launched side by side, and such as, can be set on solid space, layering arranges fish jar 1.
In the embodiment of the present invention, as embodiment, under fish jar 1, doser 2 and dosing peristaltic pump 3 can lay respectively at device, in, top, medicine adds from the doser 2 in middle level, after dissolving in doser 2, mixing, by peristaltic pump 3 coutroi velocity on upper strata, thus after flowing into the fish jar 1 of lower floor, stir through magnetic stirring apparatus 8, magnetic stirring apparatus 8 can carry out up-down vibration and forward and reverse rotation, to make the medicament in inflow fish jar (tested chemistries, i.e. pollutant) water-soluble and to mix with water.Certainly, in practical application, also peristaltic pump 3 can be placed in side or the downside of doser 2.
In the embodiment of the present invention, framework is made by stainless steel, and for bracing or strutting arrangement, bottom of device can be provided with leg, for more stable apparatus for placing.Preferably, this device can further include:
Pulley, is arranged on bottom of device, and bracing or strutting arrangement drive unit move.Like this, can so that device transport, and on pulley, locker is set, after device moves to target location, locks pulley by locker, device can not be moved.
As embodiment, temperature control cylinder groove is made up of glass or macromolecular material, and fish jar is made up of glass.
In the embodiment of the present invention, starting material, the pipe fittings such as the metal selected by semi-static temperature controlled toxicity test device of fishes, glass, plastics and the small controlling equipment selected, such as, dosing peristaltic pump, temperature sensor etc., the requirement of correlated quality control and experiment stores management should be met, for the harmless material of biology, possess acid and alkali-resistance, the characteristic that anticorrosive and chemical stability is strong.
As optional enforcement, the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention, the space taken is about 5-100m
2or can be larger, be suitable for power supply: 220V/50HZ and maybe can increase, environmental Kuznets Curves temperature: 5-45 DEG C, meanwhile, needs the plumbing interface and the use in fish breeding water processing establishment that arrange coupling at lab space.
Utilize the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention, water temperature control can be carried out, dissolved oxygen DO controls, and lower water temperature is remained to stationary temperature; By arranging electric control box, all controls of this semi-static temperature controlled toxicity test device of fishes being concentrated in electric control box, intelligentized temperature can be realized and control, manipulating comparatively simple; Further, organize fish jar by arranging more, and arrange often organize fish jar be in equivalent environment, different group fish jar can arrange different experiments parameter, can batched operation be realized, can be used for the Aquatic Ecotoxicology testing inspection of environmental chemicals, especially semi-static toxicity test of fishes.
Below the semi-static temperature controlled toxicity test device of fishes principle of work of the embodiment of the present invention and flow process are described in detail.
Fig. 3 is the semi-static Temperature Control Type toxicity test of fishes method flow schematic diagram based on the semi-static temperature controlled toxicity test device of fishes of the embodiment of the present invention.See Fig. 3, this flow process comprises:
Step 301, according to semi-static Temperature Control Type toxicity test of fishes requirement, the fish jar group number needed for pre-configured test and often organize required fish jar number;
In this step, before on-test, need according to test the preparation carrying out fish jar.Wherein,
According to toxicity test of fishes basic demand, such as, according to the requirement of the ecological toxicology testing standards such as 202,203 Magna classes, fish in OECD standard and China's chemicals method of testing, toxicity test of fishes arranges at least 6 test group, each test group arranges at least 3 fish jars arranged in parallel, certainly, in practical application, the fish jar quantity of experimental group number and test group can increase and decrease according to actual conditions, and each test group is positioned at a temperature control cylinder groove.In each test group, comprise 1 contrast fish jar, 2 test dosing (tested chemistries) fish jars, contrast fish jar composition contrast fish jar group, comprises 6 fish jars, and test dosing fish jar composition test dosing fish jar group, comprises 10 fish jars.In practical application, also according to actual tests requirement, the fish jar number in each test dosing fish jar group can be increased, or, increase the experimental group number of semi-static temperature controlled toxicity test device of fishes.Certainly, in practical application, also can according to other basic demand in toxicity test of fishes, each test group arranges 4 parallel experiment fish jars.
In the embodiment of the present invention, the actual tests water capacity of each fish jar is 5L, at least can hold 10 test fishes being less than 4g and carry out semi-static exposure test, test load < 1g/L, this test load is the test load of defined in the Organization for Economic Cooperation and Development (OECD, OrganizationforEconomicCo-operationandDevelopment) standard and China's chemicals method of testing.Certainly, in practical application, the test water capacity of fish jar also can be increased and decreased in proportion to carry out the toxicity test of the toxicologic index of different building shape fish.
After configuring and often organizing required fish jar number, laying temperature sensor in the Container arranged in fish jar, configures corresponding well heater, and is connected to electric control box.
Step 302, tests water, preheating fish jar according in the test water capacity preparation fish jar pre-set;
In this step, after test water capacity is determined, according to determined test water capacity, add water in fish jar, after having added water, the water temperature of temperature sensor senses conducts to electric control box, starts well heater by electric control box, preheating is carried out to reach the optimum temperature needed for test organism (such as, fish) to the test aqueous solution in fish jar.Like this, carry out the process of temperature Automated condtrol by electric control box, temperature sensor and well heater, the consistent and mobile equilibrium of water temperature in fish jar in all test group and each test group can be kept.
Step 303, tests water in the fish jar to configuration and carries out oxygenation and aeration;
In this step, in fish jar warm or after preheating completes, oxygenation and aeration can be carried out to the test water in fish jar, to reach the required dissolved oxygen DO requirement of toxicity test of fishes standard.
In the embodiment of the present invention, oxygenation aerator can be adopted to carry out the oxygenation of test water.
Step 304, according to testing requirements, starts doser, peristaltic pump and magnetic stirring apparatus, carries out dosing to the test water of oxygenation;
In this step, after fish jar preheating, oxygenation complete, dosing is carried out to test water.Specifically, adopt doser, carry out dosing by dosing peristaltic pump and pipeline to fish jar, according to experimental concentration requirement, can by dosing flow set at 0.1ml-10ml/min.Certainly, in practical application, also according to actual demand, while increase fish jar test aqueous solution capacity, the flow parameter of dosing peristaltic pump can be scaled up.After dosing completes, magnetic stirring apparatus is adopted fully to mix test aqueous solution in fish jar.
In the embodiment of the present invention, the toxicity of research water pollutant, generally adopts variable concentrations clean water being made into water pollutant to carry out simulation test.Thus, when preparing test aqueous solution in fish jar, the notably solubleness of different pollutant in water and stability.To the pollutant of indissoluble, cosolvent or emulsifying agent hydrotropy can be added in the test aqueous solution testing fish jar in dosing fish jar group, it should be noted that, also the cosolvent or the emulsifying agent that add corresponding dosage in the test aqueous solution of fish jar in contrast fish jar group is needed, to ensure the consistance of test condition.
Institute's step 303 that it should be noted that and step 304 do not have dividing of sequencing.
Step 305, puts into tested fish in fish jar, carries out semi-static toxicity test of fishes;
In this step, put into tested fish in fish jar, 10 juvenile fish placed by each fish jar, and so far, semi-static Temperature Control Type toxicity test of fishes starts.
Step 306, according to toxicity test of fishes standard, carries out Illumination adjusting by candling;
In this step, in whole toxicity test of fishes process, according to toxicity test of fishes standard, candling is adopted to carry out intensity, the robotization in the cycle adjustment of illumination.
Step 307, accumulative test period, if accumulative test period is less than the test period threshold value pre-set, according to the monitoring periods pre-set, change the water yield of predetermined volume in fish jar, return and perform step 303, if accumulative test period equals the test period threshold value pre-set, terminate test.
In this step, in toxicity test of fishes, according to the monitoring periods pre-set, such as, every 24h once changes water, changes the water yield that water changes predetermined volume in fish jar at every turn, is referred to as semi-static toxicity test of fishes.In the embodiment of the present invention, adopt flow control mouth to discharge 50% test aqueous solution, then return and perform step 302, with complementary testing aqueous solution and carry out concentration adjustment.
In practical application, in semi-static toxicity test of fishes process, quantity of exchanged water and change water frequency and can regulate according to the stability of tested chemistries (dosing).
In the embodiment of the present invention, for tested chemistries, test fingerling and test water, must be in the toxicology test concentration range required (being usually less than 1000mg/L), tested chemistries adopts and can directly be dissolved in test water, or can be dissolved in the inorganic or organic chemicals in test water after cosolvent effect completely.
Further, for effumability tested chemistries in water, the mode increasing and change water frequency can be adopted in experimental period (test period threshold value), guarantee that the concentration change of tested chemistries controls in ± 20% scope.
As embodiment, in experimental period, bad behavior or the mortality ratio of the tested fish (>=10) in contrast fish jar group should control within 10%.Test water generally adopts through dechlorination and the Filtered tap water of sofening treatment, or in pure water, add some mineral elements formulated, and the leading indicator of test water is as follows:
PH=6.5-8.5, dissolved oxygen DO > 5.0, conductivity=200-400u Ω/cm.
The foregoing is only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a semi-static temperature controlled toxicity test device of fishes, this device comprises: semi-static control module, toxicity test unit, heated for controlling temperature unit, aeration aerating unit and light illuminating unit, wherein,
Semi-static control module, for after monitoring and putting into tested fish in the fish jar after dosing, accumulative test period, if accumulative test period is less than the test period threshold value pre-set, according to the monitoring periods pre-set, change the water yield of predetermined volume in fish jar, test water in notice toxicity test unit preparation fish jar, if accumulative test period equals the test period threshold value pre-set, terminate test;
Toxicity test unit, for according to semi-static Temperature Control Type toxicity test of fishes requirement, the fish jar group number needed for pre-configured test and often organize required fish jar number; Water is tested according in the test water capacity preparation fish jar pre-set; According to semi-static Temperature Control Type toxicity test of fishes requirement, dosing is carried out to the test water in fish jar; Put into tested fish in fish jar after dosing, carry out semi-static toxicity test of fishes;
Heated for controlling temperature unit, for according to the test water temperature of testing in the fish jar that obtains, controls test water temperature in fish jar in the temperature range pre-set;
Aeration aerating unit, carries out oxygenation and aeration for testing water in the fish jar to configuration;
Light illuminating unit, for according to toxicity test of fishes standard, carries out Illumination adjusting by candling to toxicity test unit.
2. device as claimed in claim 1, described toxicity test unit comprises: fish jar, doser, peristaltic pump, framework and flow control mouth, wherein,
The quantity of fish jar is multiple, on the first dividing plate that placement is arranged in the frame, bottom fish jar, offers for controlling the flow control mouth that in fish jar, current are come in and gone out;
The quantity of doser is multiple, and on the second partition that placement is arranged in the frame, described second partition is positioned at above described first dividing plate, and each fish jar is to there being a doser, and doser is connected with fish jar by peristaltic pump;
3. device as claimed in claim 2, described heated for controlling temperature unit comprises: well heater, electric control box and temperature sensor, wherein,
Described well heater is placed in below described first dividing plate or an example;
Temperature sensor is arranged in fish jar;
Electric control box, connects well heater and temperature sensor respectively by electrical conduits.
4. device as claimed in claim 3, described electric control box comprises: temperature-sensitive sticker, mapping relations table storer, water temperature processor, switch and power device, wherein,
Temperature-sensitive sticker, for receiving the information of temperature sensor transmission, is converted to temperature information by the information of reception, exports the temperature sensor mark of temperature information and the information of reception to water temperature processor;
Mapping relations table storer, for arranging and the mapping relations table that in memory storage, each temperature sensor mark identifies with respective heater;
Water temperature processor, for the temperature information according to reception, determine that temperature is lower than the first temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, turn-on power device identifies the switch between corresponding well heater with the well heater of mapping; Determine that temperature is lower than the second temperature threshold pre-set, according to the temperature sensor mark received, the mapping relations table stored in query mappings relation table storer, obtain the well heater mark of the temperature sensor identity map received, deenergization device identifies the switch between corresponding well heater with the well heater of mapping.
5. device as claimed in claim 3, described well heater is arranged in the temperature control cylinder groove of framework, fish jar inwall arranges one for the containing tube of accommodating temperature sensor, described containing tube is lower than the water surface in fish jar, or, a described containing tube part immerses the water surface in fish jar, and offers through hole in the part immersing the water surface in fish jar.
6. device as claimed in claim 1, described aeration aerating unit comprises: oxygenation aerator, and described oxygenation aerator is communicated in fish jar by air shooter; Described light illuminating unit comprises: candling, and described candling is arranged at the top of framework.
7. device as claimed in claim 1, described device comprises further:
Magnetic stirring apparatus, described magnetic stirring apparatus is along fish jar placement direction, and slide downward enters in fish jar, or upward sliding, shifts out in fish jar.
8. device as claimed in claim 1, described fish jar, in the total measurement (volume) position of the aqueous solution injected, is provided with the first panel that is laid with sensor, in the total measurement (volume) half position of the aqueous solution injected, is provided with the second panel that another is laid with sensor.
9. device as claimed in claim 8, described device comprises further:
Float, be arranged between the first panel and the second panel, after described float and the second plate contact, trigger the sensor arranged in the second panel and export to semi-static control module the steering order that adds water, after described float and the first plate contact, trigger the sensor arranged in the first panel and export to semi-static control module the open command that adds water.
10. device as claimed in claim 1, described semi-static control module comprises: monitor, timer, processor and water-feed, wherein,
Monitor, for after monitoring and putting into tested fish in the fish jar after dosing, sends initiate notification to timer;
Timer, for after receiving initiate notification, starts timing, accumulative test period; After receiving off-test notice, carry out clearing process;
Processor, for according to the monitoring periods pre-set, read the timing in timer, judge whether accumulative test period is less than the test period threshold value pre-set, if so, test water in notice toxicity test unit preparation fish jar, and after monitoring the test water capacity in toxicity test unit fish jar and reducing predetermined volume, start water-feed, after monitoring water-feed inject the aqueous solution of predetermined volume in fish jar, turn off water-feed;
Water-feed, is provided with the water supply hose for supplying water to fish jar, deeply in fish jar.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061069A (en) * | 2018-08-29 | 2018-12-21 | 华南农业大学 | A kind of snail and fish eco-toxicity in-situ monitoring device and detection method |
| CN109254132A (en) * | 2018-10-12 | 2019-01-22 | 中国环境科学研究院 | A kind of microcosm experiment device for simulating pond ecosystem |
| CN110150186A (en) * | 2019-06-14 | 2019-08-23 | 中国水产科学研究院黑龙江水产研究所 | A kind of integrated experimental device and operating method for toxicity in fish research |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101424678A (en) * | 2008-11-25 | 2009-05-06 | 烟台迪特商贸有限公司 | Water quality mutation biology behavioral indicator monitoring system and monitoring method |
| CN101576554A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Dynamic running water type toxicity test device of fishes |
| CN101576555A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Static temperature controlled toxicity test device of fishes |
| CN101982072A (en) * | 2010-10-11 | 2011-03-02 | 上海市环境科学研究院 | Method for testing toxicity of environmental estrogen on whitebait embryonic development |
| CN203053955U (en) * | 2012-11-28 | 2013-07-10 | 广州中国科学院沈阳自动化研究所分所 | Sewage biological toxicity on-line monitoring system |
| CN103276042A (en) * | 2013-05-27 | 2013-09-04 | 中国科学院生态环境研究中心 | Method for detecting genetic toxicity of organic pollutants in water |
| CN103392660A (en) * | 2013-07-24 | 2013-11-20 | 广东省微生物研究所 | Exposure device for fish embryos and larva fish for long-term chronic toxicity tests |
-
2014
- 2014-07-04 CN CN201410317945.6A patent/CN105301211B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101424678A (en) * | 2008-11-25 | 2009-05-06 | 烟台迪特商贸有限公司 | Water quality mutation biology behavioral indicator monitoring system and monitoring method |
| CN101576554A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Dynamic running water type toxicity test device of fishes |
| CN101576555A (en) * | 2009-03-06 | 2009-11-11 | 刘征涛 | Static temperature controlled toxicity test device of fishes |
| CN101982072A (en) * | 2010-10-11 | 2011-03-02 | 上海市环境科学研究院 | Method for testing toxicity of environmental estrogen on whitebait embryonic development |
| CN203053955U (en) * | 2012-11-28 | 2013-07-10 | 广州中国科学院沈阳自动化研究所分所 | Sewage biological toxicity on-line monitoring system |
| CN103276042A (en) * | 2013-05-27 | 2013-09-04 | 中国科学院生态环境研究中心 | Method for detecting genetic toxicity of organic pollutants in water |
| CN103392660A (en) * | 2013-07-24 | 2013-11-20 | 广东省微生物研究所 | Exposure device for fish embryos and larva fish for long-term chronic toxicity tests |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061069A (en) * | 2018-08-29 | 2018-12-21 | 华南农业大学 | A kind of snail and fish eco-toxicity in-situ monitoring device and detection method |
| CN109061069B (en) * | 2018-08-29 | 2021-08-24 | 华南农业大学 | In-situ monitoring device and detection method for ecological toxicity of snails and fishes |
| CN109254132A (en) * | 2018-10-12 | 2019-01-22 | 中国环境科学研究院 | A kind of microcosm experiment device for simulating pond ecosystem |
| CN109254132B (en) * | 2018-10-12 | 2023-11-21 | 中国环境科学研究院 | Microcosm experimental device for simulating pond ecosystem |
| CN110150186A (en) * | 2019-06-14 | 2019-08-23 | 中国水产科学研究院黑龙江水产研究所 | A kind of integrated experimental device and operating method for toxicity in fish research |
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