CN108395983B - Culture medium configuration and mineral acquisition system - Google Patents
Culture medium configuration and mineral acquisition system Download PDFInfo
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- CN108395983B CN108395983B CN201810028830.3A CN201810028830A CN108395983B CN 108395983 B CN108395983 B CN 108395983B CN 201810028830 A CN201810028830 A CN 201810028830A CN 108395983 B CN108395983 B CN 108395983B
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- 239000001963 growth medium Substances 0.000 title claims abstract description 27
- 229910052500 inorganic mineral Inorganic materials 0.000 title claims abstract description 25
- 239000011707 mineral Substances 0.000 title claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 85
- 230000007246 mechanism Effects 0.000 claims abstract description 48
- 239000002994 raw material Substances 0.000 claims abstract description 47
- 238000009826 distribution Methods 0.000 claims abstract description 31
- 238000006243 chemical reaction Methods 0.000 claims abstract description 30
- 238000003756 stirring Methods 0.000 claims abstract description 20
- 239000007787 solid Substances 0.000 claims abstract description 19
- 230000033558 biomineral tissue development Effects 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000002474 experimental method Methods 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 230000006698 induction Effects 0.000 claims abstract description 5
- 238000011177 media preparation Methods 0.000 claims abstract description 3
- 239000012528 membrane Substances 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 239000011435 rock Substances 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229940126589 solid medicine Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910001748 carbonate mineral Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a culture medium configuration and mineral acquisition system, and relates to the technical field of biological induction carbonate rock experiments. The device comprises a bacteria induced mineralization experimental device, a culture medium configuration device and a feeding device from bottom to top, wherein the bacteria induced mineralization experimental device comprises a plurality of reaction bottles arranged side by side, each reaction bottle is provided with a cock, the reaction bottles are divided into an upper chamber and a lower chamber by the cocks, the upper chamber is used for inducing mineralization experiments, the lower chamber is in an inverted funnel shape, the side wall of the lower chamber is provided with an opening, and the opening is provided with a rubber plug; the culture medium preparation device comprises a liquid preparation box and a stirring mechanism, wherein the liquid preparation box is communicated with each reaction bottle through a pipeline; the feeding device comprises a solid raw material adding mechanism, a non-filtering liquid raw material adding mechanism and a filtering liquid raw material adding mechanism which are sequentially arranged from left to right. The system of the invention ensures that the mineral distribution is more centralized, the ore taking process is more convenient, and the risk of bacteria contamination caused by ore taking is reduced.
Description
Technical Field
The invention relates to the technical field of biological induction carbonate rock experiments, in particular to a culture medium configuration and mineral acquisition system.
Background
In the current stage of carbonate rock research, the influence of solutions with different Mg/Ca and different salt concentrations on mineral property change becomes a hot point of research, the simulation experiment process of microbial induction carbonate mineral precipitation is complex, the amount of culture medium required by the experiment is large, and the time and the labor are consumed when the culture medium is configured. Meanwhile, when the minerals are taken out from the conical flask, the bottle mouth is small, the minerals at the bottom of the flask are distributed dispersedly, and the minerals are not easy to take out.
Disclosure of Invention
The invention aims to provide a culture medium configuration and mineral acquisition system, which has the advantages of simple combination, easy disassembly and assembly and easy cleaning, can simultaneously configure culture mediums with different Mg/Ca, simplifies the experimental steps and saves a large amount of time; more importantly, the system enables mineral distribution to be more concentrated and the ore taking process to be more convenient, and reduces the risk of bacterial contamination caused by ore taking.
The technical solution comprises:
a culture medium configuration and mineral acquisition system comprises a bacteria induced mineralization experimental device, a culture medium configuration device and a feeding device from bottom to top, wherein the bacteria induced mineralization experimental device comprises a plurality of reaction bottles arranged side by side, each reaction bottle is provided with a cock, the reaction bottles are divided into an upper cavity and a lower cavity by the cocks, the upper cavity is used for induced mineralization experiments, the lower cavity is in an inverted funnel shape, the side wall of the lower cavity is provided with an opening, and the opening is provided with a rubber plug;
the culture medium preparation device comprises a liquid preparation box and a stirring mechanism, wherein the liquid preparation box is communicated with each reaction bottle through a pipeline;
the feeding device comprises a solid raw material adding mechanism, a non-filtering liquid raw material adding mechanism and a filtering liquid raw material adding mechanism which are sequentially arranged from left to right, wherein the solid raw material adding mechanism comprises a solid raw material feeder which is communicated with the liquid distribution box through a pipeline;
the filtered liquid raw material adding mechanism comprises a separating funnel, a liquid storage bottle and a filter positioned at the top of the separating funnel, the filter comprises a filter body, the filter body is cylindrical, a pressure device is arranged in the upper area in the filter body, a filter membrane is arranged in the lower area of the filter body, liquid raw materials to be filtered are stored in the liquid storage bottle, after flowing into the filter body, the liquid raw materials are filtered through the filter membrane under the action of the pressure device, a through hole is formed in the bottom of the filter body, the filtered liquid raw materials flow into the separating funnel through the through hole, and the separating funnel is communicated with the liquid distribution box;
the non-filtering liquid raw material adding mechanism is communicated with the liquid distribution box.
As a preferable scheme of the present invention, the stirring mechanism includes a stirring shaft and a paddle located on the stirring shaft, the stirring shaft is transversely disposed in the liquid distribution box, and the stirring mechanism is detachably connected to the liquid distribution box.
As another preferable scheme of the present invention, a pipeline connecting the liquid distribution tank and the reaction flask is divided into a main pipeline and a branch pipeline, an electronic switch is disposed on the main pipeline, and a flow meter is disposed on the branch pipeline.
Further, each reaction flask was equipped with a pH meter.
Furthermore, the bottom in the lower cavity of each reaction bottle is a concave surface.
Further, the solid raw material adding mechanism is also provided with an electronic scale.
Compared with the prior art, the culture medium configuration and mineral acquisition system obtained by combining the bacteria induced mineralization experimental device, the culture medium configuration device and the feeding device has the following beneficial technical effects:
(1) culture media with different Mg/Ca can be prepared simultaneously, so that the experimental steps are simplified;
(2) the bacteria induction mineralization experimental device arranged at the bottom is characterized in that the reaction bottle is divided into an upper cavity and a lower cavity, the lower cavity is in an inverted funnel shape, so that ores are conveniently taken, and inconvenience caused by the steps of inclined ore taking and the like in the prior art is solved.
(3) The culture medium configuration and mineral acquisition system is tightly connected in front and back, so that the risk of contamination is reduced, and the test error is reduced.
Drawings
The invention is further described below with reference to the accompanying drawings:
FIG. 1 is a schematic diagram of a media configuration and mineral acquisition system according to the present invention;
in the figure, 1, a solid raw material adding mechanism, 2, a non-filtered liquid raw material adding mechanism, 3, a filtered liquid raw material adding mechanism, 4, an electronic scale, 5, a switch, 6, a pressure gauge, 7, a liquid storage bottle, 8, a filter membrane, 9, a liquid distribution box, 10, a stirring mechanism switch, 11, a stirring mechanism, 12-1, 12-2, an electronic switch, 13, a flow meter, 14, a reaction bottle, 15, a pH meter, 16, a rubber plug, 17 and a cock.
Detailed Description
The invention provides a culture medium configuration and mineral acquisition system, and in order to make the advantages and technical scheme of the invention clearer and clearer, the invention is described in detail with reference to specific embodiments.
The invention relates to a culture medium configuration and mineral acquisition system, which comprises a bacteria induced mineralization experimental device, a culture medium configuration device and a feeding device from bottom to top as shown in figure 1.
Wherein, all can dismantle between induced mineralize mineralization experimental apparatus of bacterium, culture medium configuration device and the feeding device, be convenient for experimental along with the dress and tear open. The bacteria induced mineralization experimental device comprises a plurality of reaction bottles 14 arranged side by side, wherein each reaction bottle 14 is provided with a cock 17, the cock 17 is positioned at the middle lower part of the reaction bottle, the reaction bottle 14 is divided into an upper chamber and a lower chamber by the cock 17, the upper chamber is used for induced mineralization experiments, the lower chamber is in an inverted funnel shape, the side wall of the lower chamber is provided with an opening, and the opening is provided with a rubber plug 16;
the culture medium configuration device comprises a liquid distribution box 9 and a stirring mechanism 11, wherein the liquid distribution box is communicated with each reaction bottle 14 through a pipeline; the feeding device comprises a solid raw material adding mechanism 1, a non-filtered liquid raw material adding mechanism 2 and a filtered liquid raw material adding mechanism 3 which are sequentially arranged from left to right, wherein the solid raw material adding mechanism comprises a solid raw material feeder, the solid raw material feeder is provided with an electronic scale 4 for weighing solid materials, and the solid raw material feeder is communicated with a liquid distribution box 9 through a pipeline;
filter liquid raw materials and add mechanism 3 and include a separating funnel, stock solution bottle 7 and be located the filter at separating funnel top, the filter includes filter body, filter body is cylindrical, this internal upper region of filter is provided with pressure gauge 6, its below region is provided with filter membrane 8, the liquid raw materials that need to filter are stored in the stock solution bottle, after flowing into filter body, filter through filter membrane 8 under pressure gauge 6's effect, be provided with the through-hole bottom filter body, liquid raw materials after the filtration flow into separating funnel through the through-hole.
The working method of the culture medium configuration and mineral acquisition system of the present invention will be described in detail below.
Referring to fig. 1, a solid raw material adding mechanism 1 is communicated with a liquid distribution box 9 through a pipeline, and after solid medicines are weighed by an electronic scale in the solid raw material adding mechanism, a switch 5 is turned on to add the solids into the liquid distribution box 9; liquid medicines which do not need to be filtered are added by the non-filtered liquid raw material adding mechanism 2, the lower cock is opened to enable the liquid medicines to flow into the cavity on the left side of the liquid distribution box 9 to be mixed with the solid medicines added from the solid raw material adding mechanism, and the right cock is opened to enable the liquid medicines which do not need to be mixed to flow into the cavity on the right side of the liquid distribution box 9; the filter liquid raw material adds and is equipped with pressure gauge 6 and stock solution bottle 7 on the mechanism 3, and the cock is opened after the stock solution bottle 7 is added to the medicine that needs to filter, and liquid flows in pressure gauge 6, closes the cock, and liquid passes through filter membrane 8 under the pressure gauge effect, and filter membrane 8 can be pulled down the change, opens the cock that filters between liquid raw material adds the mechanism 3 and the liquid distribution box 9, and control liquid flows in the cavity on liquid distribution box 9 right side.
After the medicine is added into the liquid preparation tank 9, the switch 5 and the cocks between the unfiltered liquid raw material adding mechanism 2 and the filtered liquid raw material adding mechanism 3 and the liquid storage tank are closed, the left stirring mechanism switch 10 is opened, and the stirring mechanism 11 starts to work; after the culture medium in the left cavity in the tank is uniformly stirred and the liquid (such as magnesium solution) in the right cavity is added, the liquid distribution tank 9 is detached, the stirring mechanism switch 10 is detached by detaching the internal interface, then the liquid distribution tank 9 is sterilized, the liquid distribution tank is cooled to room temperature after sterilization, and is installed at the original position again, and the stirring mechanism switch 10 is connected through the internal interface; opening an electronic switch 12-1 on the left side of the bottom of a liquid distribution box 9, setting parameters of a flow meter 13 to enable liquid in a cavity on the left side of the liquid distribution box 9 to be added into a reaction bottle 14, then closing the electronic switch 12-1 on the left side, opening an electronic switch 12-2 on the right side, setting parameters of each flow meter to enable solution in a box body on the right side of the liquid distribution box 9 to be added into the reaction bottle in different amounts, and then closing the electronic switch 12-2; the pH meter 15 in the reaction bottle 14 can measure the pH, the hydrochloric acid liquid is still added by the unfiltered liquid raw material adding mechanism 2 when the pH is adjusted, the liquid is added into the liquid distribution box 9 through a right pipeline by controlling the cock, and the sterilization treatment is not needed at the moment; opening an electronic switch 12-2, and controlling the liquid dosage by adjusting a flowmeter to adjust the pH value in the culture flask; in the microorganism induced mineralization experiment, the adding operation of the bacterial liquid is the same as that of hydrochloric acid.
After the culture medium is prepared and the bacterial liquid is added, the reaction bottle is taken down and placed into a proper temperature for shake culture, and the pH meter can measure the pH of the liquid at any time. Take out the mineral after the certain time, the mineral that microorganism induced out can gather in the back taper mouth in the reaction flask when getting the mineral, wrench movement cock, the mineral falls into the below recess, close the cock, open the rubber buffer, take out the mineral, the rubber buffer is installed, put back the original place with the reaction flask again, such design makes the mineral more concentrated, needn't incline the rest and get the ore, avoided getting the loaded down with trivial details of ore many times, the time is saved, the risk of contracting the fungus has been reduced simultaneously, it is more accurate to make the experimental result.
It is intended that any equivalents, or obvious variations, which may be made by those skilled in the art in light of the teachings herein, be within the scope of the present invention.
Claims (4)
1. The utility model provides a culture medium configuration and mineral acquire system, its includes bacterial induction mineralization experimental apparatus, culture medium configuration device and feeding device from bottom to top, its characterized in that:
the bacteria induced mineralization experimental device comprises a plurality of reaction bottles arranged side by side, wherein each reaction bottle is provided with a cock which divides the reaction bottle into an upper chamber and a lower chamber, the upper chamber is used for induced mineralization experiments, the lower chamber is in an inverted funnel shape, an opening is arranged on the side wall of the lower chamber, and a rubber plug is matched with the opening; each reaction bottle is provided with a pH meter;
the culture medium preparation device comprises a liquid preparation box and a stirring mechanism, wherein the liquid preparation box is communicated with each reaction bottle through a pipeline;
the feeding device comprises a solid raw material adding mechanism, a non-filtering liquid raw material adding mechanism and a filtering liquid raw material adding mechanism which are sequentially arranged from left to right, wherein the solid raw material adding mechanism comprises a solid raw material feeder which is communicated with the liquid distribution box through a pipeline;
the filtered liquid raw material adding mechanism comprises a separating funnel, a liquid storage bottle and a filter positioned at the top of the separating funnel, the filter comprises a filter body, the filter body is cylindrical, a pressure device is arranged in the upper area in the filter body, a filter membrane is arranged in the lower area of the filter body, liquid raw materials to be filtered are stored in the liquid storage bottle, after flowing into the filter body, the liquid raw materials are filtered through the filter membrane under the action of the pressure device, a through hole is formed in the bottom of the filter body, the filtered liquid raw materials flow into the separating funnel through the through hole, and the separating funnel is communicated with the liquid distribution box;
the non-filtering liquid raw material adding mechanism is communicated with the liquid distribution box.
2. The system of claim 1, wherein the system further comprises: the stirring mechanism comprises a stirring shaft and a paddle positioned on the stirring shaft, the stirring shaft is transversely arranged in the liquid distribution box, and the stirring mechanism is detachably connected with the liquid distribution box.
3. The system of claim 1, wherein the system further comprises: the pipeline for connecting the liquid distribution box and the reaction bottle is divided into a main pipeline and a branch pipeline, an electronic switch is arranged on the main pipeline, and a flow meter is arranged on the branch pipeline.
4. The system of claim 1, wherein the system further comprises: the solid raw material adding mechanism is also provided with an electronic scale.
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CN201810028830.3A CN108395983B (en) | 2018-01-12 | 2018-01-12 | Culture medium configuration and mineral acquisition system |
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CN201810028830.3A CN108395983B (en) | 2018-01-12 | 2018-01-12 | Culture medium configuration and mineral acquisition system |
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CN108395983B true CN108395983B (en) | 2021-09-14 |
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CN109468221A (en) * | 2018-12-14 | 2019-03-15 | 山东科技大学 | A kind of experimental provision of indoor simulation Microbial Carbonates |
CN109593644A (en) * | 2019-02-19 | 2019-04-09 | 山东科技大学 | A kind of device for Halophiles induction carbonate mineral |
CN112322461B (en) * | 2020-10-29 | 2021-10-01 | 河北地质大学 | Experimental device for indoor simulation microorganism carbonate rock |
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CN103255048A (en) * | 2013-04-19 | 2013-08-21 | 北京科技大学 | Device and method for fixing carbon dioxide by utilizing biomineralization of spirulina platensis in seawater system |
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