CN210287374U - Automatic change filterable three-dimensional cell culture device of liquid - Google Patents
Automatic change filterable three-dimensional cell culture device of liquid Download PDFInfo
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- CN210287374U CN210287374U CN201920967570.6U CN201920967570U CN210287374U CN 210287374 U CN210287374 U CN 210287374U CN 201920967570 U CN201920967570 U CN 201920967570U CN 210287374 U CN210287374 U CN 210287374U
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Abstract
The utility model discloses a three-dimensional cell culture device with automatic liquid changing and filtering, which comprises a liquid inlet bottle, a culture bottle, a liquid inlet peristaltic pump, a liquid outlet bottle and an automatic controller; the liquid inlet bottle is communicated with an inlet of the liquid inlet peristaltic pump through a pipeline, the culture bottle is provided with a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is communicated with an outlet of the liquid inlet peristaltic pump through a pipeline, the liquid outlet pipe is communicated with an inlet of the liquid outlet peristaltic pump through a pipeline, and the liquid outlet bottle is communicated with an outlet of the liquid outlet peristaltic pump through a pipeline; the peripheral wall of the culture bottle is provided with a heating blanket, and the liquid inlet peristaltic pump, the liquid outlet peristaltic pump and the heating blanket are all connected with an automatic controller. The utility model has the characteristics of automatic change liquid and filter, sequencing and set up remaining liquid in culture solution sedimentation time interval, the automatic emptying pipe.
Description
Technical Field
The utility model belongs to the technical field of the biological equipment technique and specifically relates to an automatic change filterable three-dimensional cell culture device of liquid.
Background
The spinner flask is a container made of glass or plastic for culturing cells, and the surface of the spinner flask is treated by a certain method to be charged. The middle of the rotary bottle is provided with a shaft with a paddle, and the rotary bottle is placed on a rotary bottle machine during use. The bottle rotating machine drives the blades to rotate, so that the liquid is mixed. It is a simplified bioreactor since it changes the culture from static to dynamic, and is therefore usually used as an amplified simulation of a bioreactor. It is suitable for culture of suspension cell and adherent cell. In the prior art, a spinner flask is mostly used as a container for culturing cells, but when the cell culture is carried out by a cell spinner flask culture method, the culture solution needs to be manually replaced for a certain time to adjust the pH value. The labor cost is increased, external pollution is easily introduced when liquid is changed, and the experiment failure is easily caused by misoperation. Since the spinner flask does not have the function of automatically adjusting the ph value of the culture solution, the culture solution cannot reach the environment most suitable for the survival of cells.
In the process of three-dimensional cell culture of microcarrier, the automatic liquid change must satisfy the separation of carrier material and the liquid of changing out, avoids in the liquid change process, discharges the cell carrier that is being cultivated and waste liquid together, leads to the loss of cell product, consequently, improves three-dimensional cell culture device and optimizes its function, is the important scientific research direction who improves the success rate of cultured cell, reduces manpower and materials cost.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that an automatic change filterable three-dimensional cell culture device of liquid is provided, have the automatic liquid that trades and filter, the characteristics of remaining liquid in sequencing setting culture solution sedimentation time interval, the automatic clearance pipe.
In order to solve the technical problem, the utility model discloses the technical scheme who adopts is: a three-dimensional cell culture device with automatic liquid changing and filtering comprises a liquid inlet bottle, a culture bottle, a liquid inlet peristaltic pump, a liquid outlet bottle and an automatic controller; the liquid inlet bottle is communicated with an inlet of the liquid inlet peristaltic pump through a pipeline, the culture bottle is provided with a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is communicated with an outlet of the liquid inlet peristaltic pump through a pipeline, the liquid outlet pipe is communicated with an inlet of the liquid outlet peristaltic pump through a pipeline, and the liquid outlet bottle is communicated with an outlet of the liquid outlet peristaltic pump through a pipeline; the outer peripheral wall of the culture bottle is provided with a heating blanket, and the liquid inlet peristaltic pump, the liquid outlet peristaltic pump and the heating blanket are connected with an automatic controller.
According to the three-dimensional cell culture device with the automatic liquid changing and filtering functions, the automatic controller comprises a PLC (programmable logic controller), and a liquid inlet peristaltic pump motor and a liquid outlet peristaltic pump motor are controlled by the PLC; the culture bottle is provided with a temperature sensor and is connected with a PLC (programmable logic controller), and the heater of the heating blanket is controlled by the PLC.
The automatic change filterable three-dimensional cell culture device of liquid, automatic control includes liquid ration controller and electronic potential difference meter, feed liquor peristaltic pump motor passes through liquid ration controller control with play liquid peristaltic pump motor, the blake bottle is equipped with temperature sensor and is connected with the electronic potential difference meter, the heater of heating blanket passes through the control of electronic potential difference meter.
The three-dimensional cell culture device capable of automatically changing and filtering the liquid further comprises an air filtering membrane, wherein the upper ports of the liquid inlet bottle and the liquid outlet bottle 11 are respectively provided with an exhaust hole, and the exhaust holes are respectively provided with the air filtering membrane.
The three-dimensional cell culture device with automatic liquid changing and filtering functions is characterized in that the pipelines are silica gel hoses.
The three-dimensional cell culture device capable of automatically changing and filtering the liquid further comprises a control box body, wherein the liquid inlet peristaltic pump, the liquid outlet peristaltic pump and the automatic controller are all installed in the control box body.
The utility model provides a three-dimensional cell culture device with automatic liquid changing and filtering, which is provided with a liquid inlet bottle, a culture bottle, a liquid inlet peristaltic pump, a liquid outlet bottle and an automatic controller; the liquid inlet bottle is communicated with an inlet of the liquid inlet peristaltic pump through a pipeline, the culture bottle is provided with a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is communicated with an outlet of the liquid inlet peristaltic pump through a pipeline, the liquid outlet pipe is communicated with an inlet of the liquid outlet peristaltic pump through a pipeline, and the liquid outlet bottle is communicated with an outlet of the liquid outlet peristaltic pump through a pipeline; the heating blanket is arranged on the peripheral wall of the culture bottle, and the liquid inlet peristaltic pump, the liquid outlet peristaltic pump and the heating blanket realize the automatic operation of the culture device through the automatic controller. The cell culture process does not need manual intervention, the time, the times, the volume and the like of liquid change are set by inputting on the automatic controller by a user, and the aim of filtering and separating the liquid and the carrier in the three-dimensional cell culture liquid drainage process based on the microcarrier is fulfilled. The beneficial technical effects are as follows: the liquid changing amount and time are set by an operator through a human-computer interaction interface, the whole process can be completely automated, and compared with the traditional mode of manually supplementing/discharging liquid by using an injector, the method greatly reduces the labor force and solves the problem of bacteria contamination or nonuniform quality caused by human factors.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
The parts in the figures are numbered: the culture bottle comprises a culture bottle 1, a liquid inlet pipe 2, a liquid inlet bottle 3, a liquid inlet bottle exhaust hole 4, an air filtering membrane 5, a silica gel hose 6, a liquid inlet peristaltic pump 7, a control box body 8, a liquid outlet peristaltic pump 9, a liquid outlet bottle exhaust hole 10, a liquid outlet bottle 11, a liquid outlet pipe 12, a heating blanket 13, a liquid filtering ball 14, a paddle 15 and a stirring shaft 16.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
As shown in fig. 1, the utility model discloses a use method: the culture of the cells is carried out in the culture bottle 1, the culture bottle 1 is an isolation glass jar, and a layer of heating blanket 13 is adhered on the outer wall of the culture bottle 1 and used for keeping the liquid in the culture bottle 1 in a constant temperature state all the time. One side of the culture bottle 1 is provided with an air inlet hole and an air outlet hole for introducing gas into the culture bottle 1 to enable the cells inside to survive and discharge useless air. The left side and the right side of the culture bottle 1 are respectively provided with a liquid inlet pipe 2 and a liquid outlet pipe 12, wherein the liquid inlet pipe 2 is used for supplementing nutrients required by cells into the culture bottle 1 and adjusting the ph value or other environments of the internal liquid, and the liquid outlet pipe 12 is used for discharging metabolites of the cells. The feed liquor pipe 2 of blake bottle 1 is connected to the export of feed liquor peristaltic pump 7 through silica gel hose 6, and the entry of feed liquor peristaltic pump 7 is connected to liquid inlet bottle 3 through silica gel hose 6, and liquid inlet bottle 3 has two holes, and a hole is used for discharging the culture solution, and another hole is the air vent and is connected with air filter membrane 5. The air vent of the liquid inlet bottle 3 has the function that after the liquid in the liquid inlet bottle 3 is discharged by the liquid inlet peristaltic pump 7, the liquid in the liquid inlet bottle 3 is reduced, and the originally discharged volume needs to be supplemented by air to keep the pressure in the bottle constant. The air filtering membrane 5 is used for filtering the air entering the liquid inlet bottle 3 for one time so as to ensure the cleanliness of the air. The liquid outlet pipe 12 of the culture bottle 1 is connected to the inlet of the liquid outlet peristaltic pump 9 through the silica gel hose 6, and the outlet of the liquid outlet peristaltic pump 9 is connected to the liquid outlet bottle 11 through the silica gel hose 6. The liquid outlet bottle 11 is provided with two holes, one hole is used for discharging the culture solution, and the other hole is a vent hole and is connected with an air filtering membrane 5. So far, the liquid in the culture bottle 1 can be fed in and out through the liquid inlet peristaltic pump 7 and the liquid outlet peristaltic pump 9, and new liquid is automatically added into the culture bottle 1 or waste liquid is discharged out of the culture bottle 1.
Programmed settling interval and filtering drainage operation process: the liquid inlet bottle 3, the culture bottle 1, the liquid outlet bottle 11, the air filtering membrane 5, the liquid filtering ball 14 and the silica gel hose 6 are sterilized at high temperature and high pressure and isolated from the bacteria environment. The liquid inlet bottle 3, the culture bottle 1 and the liquid outlet bottle 11 which are subjected to sterilization treatment in the clean room need to be connected through the silica gel hose 6 by an operator, and then are integrally moved out of the clean room, and the air filtering membrane 5 can ensure that the air entering the liquid inlet bottle 3 and the air entering the liquid outlet bottle 11 have enough cleanliness. In the process of carrying out one-time complete cell culture, the times of liquid change can be set and input into the automatic controller, the set times are 0-10000 times, and the volume of each liquid change is 0-1000 ml. The liquid outlet peristaltic pump 9 can perform settlement after a period of time after all the set waste liquid is discharged, and the liquid inlet peristaltic pump 7 can perform supplement of new liquid, wherein the settlement time is 0-100 hours. According to different experimental requirements, the diameter of a peristaltic pump pipe is 1-100mm, and the rotating speed of the peristaltic pump is 6-600 revolutions per minute. The filtering holes of the filter screen of the liquid filtering ball 14 are 5-500 microns, which are smaller than the diameter of the three-dimensional cell microcarrier, thus achieving the purpose of separating the discharged liquid from the microcarrier.
Appropriate changes and modifications to the embodiments described above will become apparent to those skilled in the art from the disclosure and teachings of the foregoing description. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and changes to the present invention should fall within the protection scope of the claims of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
Claims (5)
1. The utility model provides an automatic change filterable three-dimensional cell culture device of liquid which characterized in that: comprises a liquid inlet bottle (3), a culture bottle (1), a liquid inlet peristaltic pump (7), a liquid outlet peristaltic pump (9), a liquid outlet bottle (11) and an automatic controller; the liquid feeding bottle (3) is communicated with an inlet of a liquid feeding peristaltic pump (7) through a pipeline, the culture bottle (1) is provided with a liquid feeding pipe (2) and a liquid discharging pipe (12), the liquid feeding pipe (2) is communicated with an outlet of the liquid feeding peristaltic pump (7) through a pipeline, the liquid discharging pipe (12) is communicated with an inlet of a liquid discharging peristaltic pump (9) through a pipeline, and the liquid discharging bottle (11) is communicated with an outlet of the liquid discharging peristaltic pump (9) through a pipeline; be equipped with heating blanket (13) on the periphery wall of blake bottle (1), feed liquor peristaltic pump (7), play liquid peristaltic pump (9) and heating blanket (13) are through the electricity connection automatic control.
2. The three-dimensional cell culture device with automatic liquid change and filtration as claimed in claim 1, wherein: the automatic controller adopts a PLC programmable logic controller, and the motor of the liquid inlet peristaltic pump (7) and the motor of the liquid outlet peristaltic pump (9) are both connected with the PLC programmable logic controller for control; the culture bottle (1) is provided with a temperature sensor and is connected with a PLC (programmable logic controller), and a heater of the heating blanket (13) is connected with the PLC.
3. The three-dimensional cell culture device with automatic liquid change and filtration as claimed in claim 1 or 2, wherein: still include air filter membrane (5), the last port of liquid bottle (3) and play liquid bottle (11) all is equipped with the exhaust hole air filter membrane (5) all be equipped with in the exhaust hole.
4. The three-dimensional cell culture device with automatic liquid change and filtration function according to claim 3, wherein: the pipelines are silica gel hoses (6).
5. The three-dimensional cell culture device with automatic liquid change and filtration as claimed in claim 1, wherein: the liquid inlet peristaltic pump (7), the liquid outlet peristaltic pump (9) and the automatic controller are all arranged in the control box body (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920967570.6U CN210287374U (en) | 2019-06-25 | 2019-06-25 | Automatic change filterable three-dimensional cell culture device of liquid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920967570.6U CN210287374U (en) | 2019-06-25 | 2019-06-25 | Automatic change filterable three-dimensional cell culture device of liquid |
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| CN210287374U true CN210287374U (en) | 2020-04-10 |
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| CN201920967570.6U Active CN210287374U (en) | 2019-06-25 | 2019-06-25 | Automatic change filterable three-dimensional cell culture device of liquid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115820533A (en) * | 2022-12-31 | 2023-03-21 | 王茂军 | Stem cell culture process |
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2019
- 2019-06-25 CN CN201920967570.6U patent/CN210287374U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115820533A (en) * | 2022-12-31 | 2023-03-21 | 王茂军 | Stem cell culture process |
| CN115820533B (en) * | 2022-12-31 | 2023-12-01 | 剑兰生物医学科技(辽宁)有限公司 | Stem cell culture process |
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