CN117511713B - Full-automatic fermentation tank for microbial culture medium and fermentation method thereof - Google Patents
Full-automatic fermentation tank for microbial culture medium and fermentation method thereof Download PDFInfo
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- CN117511713B CN117511713B CN202311531111.0A CN202311531111A CN117511713B CN 117511713 B CN117511713 B CN 117511713B CN 202311531111 A CN202311531111 A CN 202311531111A CN 117511713 B CN117511713 B CN 117511713B
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- 238000000855 fermentation Methods 0.000 title claims abstract description 130
- 230000004151 fermentation Effects 0.000 title claims abstract description 129
- 239000001963 growth medium Substances 0.000 title claims abstract description 19
- 238000009629 microbiological culture Methods 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 239000007789 gas Substances 0.000 claims description 32
- 238000009423 ventilation Methods 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 19
- 244000005700 microbiome Species 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 11
- 229910052760 oxygen Inorganic materials 0.000 claims description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 230000001276 controlling effect Effects 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 238000001802 infusion Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 5
- 230000000670 limiting effect Effects 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000000463 material Substances 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 10
- 230000000813 microbial effect Effects 0.000 abstract description 5
- 238000001914 filtration Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 4
- 239000002912 waste gas Substances 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention relates to the technical field of microbial fermentation, and particularly discloses a full-automatic fermentation tank for a microbial culture medium, which comprises a frame, a fermentation tank and a control box, wherein the fermentation tank is arranged at the top of the frame, a discharge pipe is arranged at the lower end of the fermentation tank, a sealing cover is arranged at an opening at the upper end of the fermentation tank in a sealing manner, a seed inlet pipe and a liquid inlet pipe are arranged on the sealing cover, a seed conveying pipe and a liquid conveying pipe are respectively connected to the seed inlet pipe and the liquid inlet pipe, the end part of the seed conveying pipe is connected with the seed tank, and the end part of the liquid conveying pipe is connected with a liquid conveying pump; a gas treatment unit is arranged on the frame beside the fermentation tank; the invention can prevent the steam from contacting the filter element cylinder in the sterilization operation process, effectively prevent the filter element cylinder from being damaged or destroyed under the influence of high-temperature steam, improve the air filtering effect of the whole fermentation tank in the fermentation process, and realize on-line quick replacement of the filter element cylinder in the whole gas treatment unit, and ensure the continuous operation of the whole fermentation process.
Description
Technical Field
The invention relates to the technical field of microbial fermentation, and particularly discloses a full-automatic fermentation tank for a microbial culture medium.
Background
Microbial fermentation refers to the process of converting a feedstock into a product desired by a human through a specific metabolic pathway under suitable conditions using microorganisms. Traditional microbial fermentation is mainly carried out in a culture dish, a glass bottle and a fermentation tank, wherein the fermentation tank is used for carrying out mass efficient fermentation on microorganisms.
In the fermentation process of using a fermentation tank as a culture medium container to ferment some aerobic microorganisms, air needs to be regularly introduced into the fermentation tank, but in order to prevent external impurities from being introduced into the fermentation tank in the air introducing process, a filter is often required to be arranged on a gas pipeline to filter the air, and meanwhile, waste gas is generated in the fermentation process of the microorganisms, and the waste gas can be discharged after the waste gas is filtered when the waste gas is discharged out of the fermentation tank.
The utility model patent with the application number 2011203778242 discloses a fermentation tank air inlet device with a filter, which comprises an air inlet pipe, wherein the air inlet pipe is connected with a fermentation tank pipeline, a coarse filter and a precise filter are sequentially arranged on the air inlet pipe along the air inlet direction, the air inlet pipeline of the precise filter is connected with a steam filter, and the steam filter is connected with an external steam source; air inlet control valves are arranged on the pipelines between the steam filter and the precise filter, between the coarse filter and the precise filter and between the precise filter and the fermentation tank; the fermentation tank disclosed in the patent can filter the air introduced into the fermentation tank through the set coarse filter and the set precise filter, but gas can be generated in the microbial fermentation culture process, and if the gas cannot be timely discharged, the gas can cause the excessive pressure inside the fermentation tank, and if the gas is directly discharged, the environment of a culture workshop can be influenced. In addition, before the fermentation tank is used for culturing microorganisms, high-temperature steam is required to be used for sterilizing the interior of the fermentation tank, the same air inlet pipeline is often adopted for introducing the high-temperature steam, the filter element in the filter is often damaged or damaged in the high-temperature steam introducing process, and meanwhile, the filter element connected with the fermentation tank can be replaced after the whole filter is separated from the pipeline in the replacing process, so that the replacing step is complex, and the quick replacement of the filter element in the filter cannot be performed on line. Based on the above, the utility model provides a full-automatic fermentation tank for a microorganism culture medium, which can effectively solve the technical problems.
Disclosure of Invention
The invention aims to provide a full-automatic fermentation tank for a microorganism culture medium, which aims to solve the technical problems of the prior fermentation tube in the background technology.
The invention is realized by the following technical scheme:
The full-automatic fermentation tank for the microorganism culture medium comprises a frame, a fermentation tank and a control box, wherein the fermentation tank is arranged at the top of the frame, a discharge pipe is arranged at the lower end of the fermentation tank, a sealing cover is arranged at an opening at the upper end of the fermentation tank in a sealing manner, a seed inlet pipe and a liquid inlet pipe are arranged on the sealing cover, a seed conveying pipe and a liquid conveying pipe are respectively connected to the seed inlet pipe and the liquid inlet pipe, the end part of the seed conveying pipe is connected with the seed tank, and the end part of the liquid conveying pipe is connected with a liquid conveying pump;
The fermentation cylinder comprises a fermentation cylinder, and is characterized in that a gas treatment unit is arranged on a frame beside the fermentation cylinder, the gas treatment unit comprises a cylindrical box, a first rotary table and a second rotary table are respectively arranged at the upper end and the lower end of an inner cavity of the cylindrical box, two symmetrical filter element barrels and two symmetrical ventilation barrels are arranged between the first rotary table and the second rotary table, a rotary switching device is arranged at the top of the cylindrical box, the lower end of the rotary switching device is connected with the first rotary table and the second rotary table, a replacement opening aligned with the two ventilation barrels is formed in the upper surface of the cylindrical box, a cover plate is arranged in the replacement opening, plug plugs connected with the two filter element barrels are respectively connected at the upper end and the lower end of the cylindrical box, a double-end cylinder for realizing synchronous approaching or keeping away from the two plug plugs is arranged on the outer side surface of the cylindrical box, an air inlet pipe and a first pipe are arranged on the plug aligned up and down, the first pipe stretches into the fermentation cylinder, the plug aligned up end of the second pipe is provided with an exhaust pipe, and the upper end of the gas pipe stretches to the fermentation cylinder is arranged at the upper end of the gas pipe.
As the further setting of above-mentioned scheme, all be annular array form and seted up four round holes on first carousel and the second carousel, two the upper end of air-vent cylinder and two symmetrical round hole fixed connection on the first carousel, the lower extreme inserts and establishes and pass the setting of round hole on the second carousel, two the filter core section of thick bamboo upper and lower extreme all runs through two other round hole settings, and is provided with the die-ring that carries out spacing to filter core section of thick bamboo lower extreme at the lower surface of second carousel.
As a further setting of the scheme, a vertical downward guide rod is connected to the first rotary table, a guide through hole matched with the guide rod is formed in the second rotary table, a one-way driver is fixedly arranged at the circle center of the first rotary table, the end part of an output shaft of the rotary switching device is connected with the one-way driver, a vertical downward reciprocating screw rod is connected to the one-way driver, and a screw rod nut matched with the reciprocating screw rod is arranged at the circle center of the second rotary table.
As a further setting of above-mentioned scheme, the one-way driver is including setting up the dish seat on first carousel, the inside center department of dish seat is provided with the ratchet that is connected with rotatory auto-change over device, is located evenly rotate in the peripheral dish seat of ratchet and be connected with a plurality of pawls, and be connected with the spring between pawl and the dish seat inner chamber lateral wall, the upper end of dish seat is provided with the lid, the upper end of reciprocating screw rod stretches into in the dish seat and is connected with the ratchet.
As a further arrangement of the scheme, the end part of the first gas pipe is connected with a three-way regulating valve, the three-way regulating valve is connected with a first branch pipe and a second branch pipe, the first branch pipe extends to the upper end of the inner cavity of the fermentation tank, and the second branch pipe extends to the lower end of the inner cavity of the fermentation tank.
As a further arrangement of the scheme, a jacket layer is arranged on the periphery of the lower end of the fermentation tank, and a first connecting pipe end and a second connecting pipe end are respectively arranged at the upper end and the lower end of the jacket layer.
As a further arrangement of the scheme, a temperature sensor, a pH value sensor and an oxygen concentration sensor which extend into the fermentation tank are arranged outside the jacket layer.
As a further arrangement of the scheme, the upper surface of the sealing cover is provided with an air pressure sensor extending to the upper end of the inner cavity of the fermentation tank, and the air pressure sensor, the temperature sensor, the pH value sensor and the oxygen concentration sensor are electrically connected with the control box.
As a further arrangement of the scheme, a stirring driving device is arranged in the center of the upper surface of the sealing cover, the lower end of the stirring driving device is connected with a stirring shaft extending into the inner cavity of the fermentation tank, and the lower end of the stirring shaft is connected with stirring blades.
The invention also discloses a fermentation method using the fermentation tank, which comprises the following steps:
1) Sterilizing the pot body: starting a rotary switching device to rotate two symmetrical air ventilation barrels to be aligned with two groups of plug plugs respectively, controlling a double-head air cylinder to enable each group of plug plugs to be close to each other and further connected with the upper end and the lower end of the air ventilation barrel in a sealing mode, then connecting the end portion of an air inlet pipe with a steam generator, sending high-temperature steam into a fermentation tank for sterilization treatment for 20-30min, and then stopping introducing the high-temperature steam;
2) Drying the pot body: after the sterilized fermentation tank is cooled to room temperature, starting the rotary switching device again to rotate the two symmetrical filter element cylinders to be aligned with the two groups of plug-in plugs respectively, controlling the double-head air cylinder to enable each group of plug-in plugs to be close to each other and further connected with the upper end and the lower end of the filter element cylinder in a sealing manner, introducing filtered air into the fermentation tank through the air pump, and discharging the air from the exhaust pipe to achieve the effect of drying the interior of the tank body;
3) And (3) liquid adding inoculation: quantitatively injecting a culture solution into the fermentation tank by starting an infusion pump, quantitatively adding strains in the seed tank through a seed inlet pipe, culturing after mixing the two strains, and controlling the temperature, the pH value, the oxygen content and the air pressure value in the fermentation tank;
4) Fermentation culture: in the fermentation process, air is regularly introduced into the fermentation tank through the air inlet pipe, meanwhile, nutrient solution is regularly supplemented into the fermentation tank, and bottling and discharging can be carried out after 3-7 days of culture.
Compared with the prior art, the invention has the following beneficial effects:
When the full-automatic fermentation tank for the microbial culture medium is subjected to steam sterilization before being put into fermentation culture, the rotary switching device in the gas treatment unit is started, so that the filter element cylinder originally connected in the gas path can be switched into the ventilation cylinder, and then high-temperature steam is introduced to perform sterilization operation, and the steam cannot be in contact with the filter element cylinder in the whole sterilization operation process, so that the filter element cylinder can be effectively prevented from being damaged or destroyed due to the influence of the high-temperature steam, the air filtering effect of the whole fermentation tank in the fermentation process is greatly improved, and the influence of external impurities on the fermentation process is avoided.
The invention further carries out improved design on the gas treatment unit, the rotary switching device is connected with the unidirectional transmission device on the first turntable, the lower end of the unidirectional transmission device is connected with the reciprocating screw rod, then the screw rod nut matched with the reciprocating screw rod is arranged on the second turntable, the switching of the filter element cylinder and the ventilation cylinder in the gas circuit can be realized in the normal clockwise rotation process of the rotary switching device, the reciprocating screw rod can synchronously rotate when the rotary switching device anticlockwise rotates, the lifting of the second turntable is realized through the transmission effect between the reciprocating screw rod and the screw rod nut, at the moment, the lower end of the filter element cylinder can move up and down along with the second turntable due to the limiting effect of the second turntable, then the filter element cylinder is automatically ejected out from the replacement port, further the online quick replacement of the filter element cylinder in the whole gas treatment unit is realized, the whole fermentation tank can normally carry out fermentation treatment in the replacement process, the continuous operation of the whole fermentation process is ensured, and the structure design is ingenious and the use effect is excellent.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a first angular perspective structure of the present invention;
FIG. 2 is a schematic view of a second angle perspective structure of the present invention;
FIG. 3 is a schematic diagram of the front view internal plane structure of the fermenter and jacket layer according to the present invention;
FIG. 4 is an enlarged schematic view of the structure of FIG. 2A according to the present invention;
FIG. 5 is a schematic perspective view of a gas treatment unit according to the present invention;
FIG. 6 is a schematic perspective view of the inside of the gas treatment unit according to the present invention;
FIG. 7 is a schematic perspective view of a first rotary disk, a second rotary disk, a filter cartridge, a ventilation cartridge and the like;
FIG. 8 is a schematic diagram of a three-dimensional structure of a filter cartridge, a double-headed cylinder, a first air delivery pipe, and the like in the present invention;
FIG. 9 is a schematic perspective view of a first turntable, a second turntable, a reciprocating screw rod and the like;
fig. 10 is a schematic perspective view of the inside of the unidirectional actuator according to the present invention.
Wherein:
100-racks, 200-fermentation tanks, 201-discharge pipes, 202-covers, 203-seed inlet pipes, 204-liquid inlet pipes, 205-seed conveying pipes, 206-infusion pipes, 207-seed tanks and 208-infusion pumps;
300-control box, 400-gas treatment unit, 401-cylindrical box, 4011-replacement port, 4012-cover plate, 402-first turntable, 403-second turntable, 404-cartridge, 405-vent cartridge, 406-rotary switching device, 407-plug, 408-double-headed cylinder, 409-inlet pipe, 410-first gas pipe, 411-exhaust pipe, 412-second gas pipe, 413-round hole, 414-backing ring, 415-guide bar, 416-unidirectional transmission, 4161-disk seat, 4162-ratchet, 4163-pawl, 4164-spring, 4165-disk cover, 417-reciprocating screw, 418-screw nut, 419-three-way regulating valve, 420-first branch pipe, 421-second branch pipe;
500-jacket layer, 501-first connecting pipe end, 502-second connecting pipe end, 503-temperature sensor, 504-pH value sensor, 505-oxygen concentration sensor, 506-air pressure sensor;
600-stirring driving device, 601-stirring shaft and 602-stirring blade.
Detailed Description
In order that those skilled in the art will better understand the present application, a technical solution in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The present application will be described in detail with reference to fig. 1 to 10, and examples thereof.
Examples
Embodiment 1 discloses a full-automatic fermentation tank for a microorganism culture medium, referring to fig. 1 and 2, which comprises a frame 100, a fermentation tank 200 and a control box 300, wherein the fermentation tank 200 is arranged at the top of the frame 100, then a discharge pipe 201 is arranged at the lower end of the fermentation tank 200, and a sealing cover 202 is arranged at the upper end opening of the fermentation tank 200 in a sealing manner through a flange and a rubber ring.
Referring to fig. 2 and 4, a seed inlet pipe 203 and a liquid inlet pipe 204 are provided on a cover 202, a seed pipe 205 and a liquid inlet pipe 206 are connected to the seed inlet pipe 203 and the liquid inlet pipe 204, respectively, then a seed tank 207 is connected to an end of the seed pipe 205, and an infusion pump 208 is connected to an end of the liquid inlet pipe 206.
Referring to fig. 1 and 3, a jacket layer 500 is provided at the periphery of the lower end of the fermenter 200, and a first coupling end 501 and a second coupling end 502 are provided at the upper and lower ends of the jacket layer 500, respectively. The inside of the jacket layer 500 may be used to introduce steam or cooling water to achieve temperature control inside the fermenter 200. A temperature sensor 503, a pH sensor 504, and an oxygen concentration sensor 505, which extend to the inside of the fermenter 200, are provided at the outside of the jacket layer 500. Then, an air pressure sensor 506 extending to the upper end of the inner cavity of the fermentation tank 200 is arranged on the upper surface of the sealing cover 202, and the air pressure sensor 506, the temperature sensor 503, the pH sensor 504 and the oxygen concentration sensor 505 are electrically connected with the control box 300. Various parameters inside the fermenter can be detected in real time by the temperature sensor 503, the pH sensor 504, the oxygen concentration sensor 505, and the air pressure sensor 506.
In addition, a stirring driving device 600 is further arranged at the center of the upper surface of the sealing cover 202, the stirring driving device 600 is composed of a motor and a speed reducer, a stirring shaft 601 extending into the inner cavity of the fermentation tank 200 is connected to the lower end of the stirring driving device 600, and then a stirring blade 602 is connected to the lower end of the stirring shaft 601.
Referring to fig. 2,5, 6 and 7, a gas treatment unit 400 is provided on the frame 100 located at the side of the fermenter 200, the gas treatment unit 400 includes a cylindrical case 401, a first rotary disk 402 and a second rotary disk 403 are respectively provided at upper and lower ends of an inner cavity of the cylindrical case 401, and then two symmetrical filter cartridges 404 and two symmetrical ventilation cartridges 405 are provided between the first rotary disk 402 and the second rotary disk 403. A rotary switching device 406 is arranged at the top of the cylindrical box 401, the rotary switching device 406 is also composed of a motor and a speed reducer, and then the lower end of the rotary switching device 406 is connected with the first rotary disc 402 and the second rotary disc 403, so that the rotary switching of the filter element barrel 404 and the ventilation barrel 405 in a gas path can be synchronously realized by the first rotary disc 402 and the second rotary disc 403 under the action of the rotary switching device 406.
Referring to fig. 5 and 8, the upper and lower ends of the cylindrical box 401 are connected with plug plugs 407 connected with two filter element barrels 404, the outer side surface of the cylindrical box 401 is provided with a double-head cylinder 408 for realizing synchronous approaching or separating of the upper and lower plug plugs 407, wherein the two plug plugs 407 aligned up and down are provided with an air inlet pipe 409 and a first air pipe 410, the first air pipe 410 extends to the interior of the fermentation tank 200, the other two plug plugs 407 aligned up and down are provided with an air outlet pipe 411 and a second air pipe 412, and the end part of the second air pipe 412 extends to the upper end of the inner cavity of the fermentation tank 200. In order to realize that air can enter at the upper end and the lower end of the fermentation tank 200 in the fermentation process, a three-way regulating valve 419 is further connected to the end part of the first air pipe 410, a first branch pipe 420 and a second branch pipe 421 are connected to the three-way regulating valve 419, the first branch pipe 420 extends to the upper end of the inner cavity of the fermentation tank 200, and the second branch pipe 421 extends to the lower end of the inner cavity of the fermentation tank 200.
The gas treatment unit 400 is designed to switch the states of the cartridge 404 and the ventilator 405 in the gas path according to the use state of the fermenter. When high-temperature steam is required to be introduced to sterilize the interior of the fermentation tank 200, the two ventilation cylinders 405 are respectively connected to the air inlet pipeline and the air outlet pipeline, and then the high-temperature steam is normally introduced to sterilize, and the sterilized steam is discharged from the air outlet pipeline. In the normal fermentation process, two filter element cylinders 404 are respectively connected to the air inlet pipeline and the air outlet pipeline, and then the air introduced and the gas discharged from the fermentation are respectively filtered through the two filter element cylinders 404.
Examples
Example 2 discloses a fully automatic fermenter for the cultivation of microorganisms, which is designed in an improved manner on the basis of the technical scheme in example 1, and the same points as in example 1 are not described again.
Referring to fig. 6, in embodiment 2, a replacement port 4011 aligned with two ventilation cylinders 405 is formed in the upper surface of a cylindrical case 401, and a cover plate 4012 is detachably provided in the replacement port 4011.
Referring to fig. 7, 8 and 9, in this embodiment 2, four circular holes 413 are formed on the first rotating disc 402 and the second rotating disc 403 in a ring array shape, then the upper ends of the two ventilation drums 405 are fixedly connected with the two symmetrical circular holes 413 on the first rotating disc 402, the lower ends of the ventilation drums are inserted and arranged through the circular holes 413 on the second rotating disc 403, the upper ends and the lower ends of the two filter element drums 404 are penetrated through the other two circular holes 413, and a supporting ring 414 for limiting the lower ends of the filter element drums 404 is arranged on the lower surface of the second rotating disc 403, so that the filter element drums 404 can be inserted and fixed in the corresponding circular holes 413 on the first rotating disc 402 and the second rotating disc 403.
A vertically downward guide bar 415 is connected to the first turntable 402, and a guide through hole matched with the guide bar 415 is formed in the second turntable 403. Meanwhile, a unidirectional driver 416 is fixedly arranged at the center of the first turntable 402, the end part of an output shaft of the rotary switching device 406 is connected with the unidirectional driver 416, a vertically downward reciprocating screw rod 417 is connected in the unidirectional driver 416, and a screw rod nut 418 matched with the reciprocating screw rod 417 is arranged at the center of the second turntable 403.
The unidirectional driver 416 includes a disc seat 4161 disposed on the first turntable 402, a ratchet 4162 connected to the rotation switching device 406 is disposed at the inner center of the disc seat 4161, a plurality of pawls 4163 are uniformly rotatably connected in the disc seat 4161 located at the periphery of the ratchet 4162, a spring 4164 is connected between the pawls 4163 and the inner cavity side wall of the disc seat 4161, a disc cover 4165 is disposed at the upper end of the disc seat 4161, and the upper end of the reciprocating screw 417 extends into the disc seat 4161 to be connected to the ratchet 4162.
In this embodiment 2, through the structural improvement of the above-mentioned gas processing unit 400, when the filter element cylinder 404 needs to be replaced online, the rotary switching device 406 is started to rotate clockwise so as to align the two filter element cylinders 404 up and down with the replacing port 4011, then the rotary switching device 406 is started reversely, the rotation of the reciprocating screw rod 417 is realized by the action of the unidirectional driver 416, then the lifting movement of the second turntable 403 is realized under the cooperation of the reciprocating screw rod 417 and the screw nut 418, then the filter element cylinder 404 automatically extends out from the replacing port 4011 in the process of moving up the second turntable 403, at this time, after the new filter element cylinder 404 is directly removed, the rotary switching device 406 is driven reversely to reset the second turntable 403, and after the replacement of the filter element cylinder 404 is completed, the rotary switching device 406 is started to rotate clockwise again so as to connect the replaced filter element cylinder 404 with the air inlet pipeline and the air outlet pipeline.
In addition, this example 2 also provides a fermentation method using the present fermenter, comprising the steps of:
firstly, sterilizing a tank body: starting a rotary switching device 406 to rotate two symmetrical air ventilation drums 405 to be aligned with two groups of plug plugs 407 respectively, controlling a double-head air cylinder 408 to enable each group of plug plugs 407 to be close to each other so as to be connected with the upper end and the lower end of the air ventilation drum 405 in a sealing mode, connecting the end portion of an air inlet pipe 409 with a steam generator, sending high-temperature steam into the fermentation tank 200 for sterilization treatment for 20-30min, and stopping introducing the high-temperature steam;
Secondly, drying the tank body: after the sterilized fermentation tank 200 is cooled to room temperature, starting the rotary switching device 406 again to rotate the two symmetrical filter element cylinders 404 to be aligned with the two groups of plug plugs 407 respectively, controlling the double-head air cylinder 408 to enable each group of plug plugs 407 to be close to each other so as to be in sealing connection with the upper end and the lower end of the filter element cylinder 404, introducing filtered air into the fermentation tank 200 through an air pump, and discharging the air from the exhaust pipe 411 to achieve the effect of drying the interior of the tank;
Thirdly, liquid adding inoculation: starting an infusion pump 208 to quantitatively inject a culture solution into the fermentation tank 200, quantitatively adding strains in a seed tank 207 through a seed inlet pipe 203, culturing after mixing the two strains, and controlling the temperature, the pH value, the oxygen content and the air pressure value in the fermentation tank 200;
Fourth, fermenting and culturing: in the fermentation process, air is regularly introduced into the fermentation tank 200 through the air inlet pipe 409, meanwhile, nutrient solution is regularly supplemented into the fermentation tank 200, and bottling and discharging can be performed after 3-7 days of culture.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (10)
1. The utility model provides a full-automatic fermentation cylinder of microorganism culture medium, includes frame (100), fermentation cylinder (200) and control box (300), fermentation cylinder (200) set up the top at frame (100), its characterized in that, the lower extreme of fermentation cylinder (200) is provided with row material pipe (201), and the upper end opening seals and is provided with closing cap (202), be provided with seed feed pipe (203) and liquid feed pipe (204) on closing cap (202), be connected with seed pipe (205) and transfer line (206) on seed feed pipe (203) and liquid feed pipe (204) respectively, the end connection of seed pipe (205) has seed tank (207), and the end connection of transfer line (206) has transfer pump (208);
The gas treatment unit (400) is arranged on the frame (100) at the side of the fermentation tank (200), the gas treatment unit (400) comprises a cylindrical box (401), a first rotary table (402) and a second rotary table (403) are respectively arranged at the upper end and the lower end of an inner cavity of the cylindrical box (401), two symmetrical filter element cylinders (404) and two symmetrical ventilation cylinders (405) are arranged between the first rotary table (402) and the second rotary table (403), a rotary switching device (406) is arranged at the top of the cylindrical box (401), the lower end of the rotary switching device (406) is connected with the first rotary table (402) and the second rotary table (403), a replacement opening (4011) aligned with the two ventilation cylinders (405) is formed in the upper surface of the cylindrical box (401), a cover plate (4012) is arranged in the replacement opening (4011), plug-in (407) connected with the two filter element cylinders (404) are connected, a plug-in plug (407) is arranged on the upper end and the lower end of the cylindrical box (401), two plug-in-plug connectors (410) are arranged on the outer side of the cylindrical box (401) respectively, two plug-in-connectors (410) are arranged on the upper side of the cylindrical box (401) or two plug-in-connectors (410) are arranged on the upper side of the cylindrical box (401) and the two plug connectors (410) and are far away from the upper side of the first plug-in the air inlet pipe (410) respectively, the plug plugs (407) aligned up and down are provided with an exhaust pipe (411) and a second gas pipe (412), and the end parts of the second gas pipe (412) extend to the upper end of the inner cavity of the fermentation tank (200).
2. The full-automatic fermentation tank for the microbial culture medium according to claim 1, wherein the first rotary table (402) and the second rotary table (403) are provided with four circular holes (413) in a ring array shape, the upper ends of the two ventilation drums (405) are fixedly connected with the two symmetrical circular holes (413) on the first rotary table (402), the lower ends of the two ventilation drums are inserted and pass through the upper circular holes (413) of the second rotary table (403) to be arranged, the upper and lower ends of the two filter element drums (404) are respectively penetrated through the other two circular holes (413), and a supporting ring (414) for limiting the lower ends of the filter element drums (404) is arranged on the lower surface of the second rotary table (403).
3. The full-automatic fermentation tank for the microbial culture medium according to claim 2, wherein the first rotary table (402) is connected with a vertical downward guide rod (415), the second rotary table (403) is provided with a guide through hole matched with the guide rod (415), a one-way driver (416) is fixedly arranged at the center of the first rotary table (402), the end part of an output shaft of the rotary switching device (406) is connected with the one-way driver (416), a vertical downward reciprocating screw rod (417) is connected in the one-way driver (416), and a screw rod nut (418) matched with the reciprocating screw rod (417) is arranged at the center of the second rotary table (403).
4. A full-automatic fermenter for microorganism culture medium according to claim 3, wherein the unidirectional actuator (416) comprises a tray seat (4161) arranged on the first turntable (402), a ratchet wheel (4162) connected with the rotary switching device (406) is arranged at the inner center of the tray seat (4161), a plurality of pawls (4163) are uniformly and rotatably connected in the tray seat (4161) arranged at the periphery of the ratchet wheel (4162), a spring (4164) is connected between the pawls (4163) and the inner cavity side wall of the tray seat (4161), a tray cover (4165) is arranged at the upper end of the tray seat (4161), and the upper end of the reciprocating screw (417) extends into the tray seat (4161) to be connected with the ratchet wheel (4162).
5. The full-automatic fermentation tank for the microbial culture medium according to claim 1, wherein the end part of the first gas pipe (410) is connected with a three-way regulating valve (419), the three-way regulating valve (419) is connected with a first branch pipe (420) and a second branch pipe (421), the first branch pipe (420) extends to the upper end of the inner cavity of the fermentation tank (200), and the second branch pipe (421) extends to the lower end of the inner cavity of the fermentation tank (200).
6. The full-automatic fermentation tank for the microbial culture medium according to claim 1, wherein a jacket layer (500) is arranged on the periphery of the lower end of the fermentation tank (200), and a first connecting pipe end (501) and a second connecting pipe end (502) are respectively arranged at the upper end and the lower end of the jacket layer (500).
7. The full-automatic fermentation tank for a microbial culture medium according to claim 6, wherein a temperature sensor (503), a pH sensor (504) and an oxygen concentration sensor (505) extending to the interior of the fermentation tank (200) are arranged at the outer part of the jacket layer (500).
8. The full-automatic fermentation tank for the microbial culture medium according to claim 7, wherein an air pressure sensor (506) extending to the upper end of the inner cavity of the fermentation tank (200) is arranged on the upper surface of the sealing cover (202), and the air pressure sensor (506), the temperature sensor (503), the pH value sensor (504) and the oxygen concentration sensor (505) are electrically connected with the control box (300).
9. The full-automatic fermentation tank for the microbial culture medium according to claim 1, wherein a stirring driving device (600) is arranged in the center of the upper surface of the sealing cover (202), a stirring shaft (601) extending into the inner cavity of the fermentation tank (200) is connected to the lower end of the stirring driving device (600), and a stirring blade (602) is connected to the lower end of the stirring shaft (601).
10. A fermentation process using the fermenter according to any one of claims 1 to 9, comprising the steps of:
Sterilizing the pot body: starting a rotary switching device (406) to rotate two symmetrical air ventilation drums (405) to be aligned with two groups of plug-in plugs (407) respectively, controlling a double-head air cylinder (408) to enable each group of plug-in plugs (407) to be close to each other so as to be connected with the upper end and the lower end of the air ventilation drum (405) in a sealing mode, then connecting the end of an air inlet pipe (409) with a steam generator, sending high-temperature steam into the fermentation tank (200) for sterilization treatment for 20-30min, and then stopping introducing the high-temperature steam;
Drying the pot body: after the sterilized fermentation tank (200) is cooled to room temperature, starting a rotary switching device (406) again to rotate two symmetrical filter element cylinders (404) to be aligned with two groups of plug plugs (407) respectively, controlling a double-head cylinder (408) to enable each group of plug plugs (407) to be close to each other and further connected with the upper end and the lower end of the filter element cylinder (404) in a sealing way, introducing filtered air into the fermentation tank (200) through an air pump, and exhausting air from an exhaust pipe (411) to achieve the effect of drying the interior of the tank;
And (3) liquid adding inoculation: quantitatively injecting a culture solution into the fermentation tank (200) by starting an infusion pump (208), quantitatively adding strains in the seed tank (207) through a seed inlet pipe (203), culturing after mixing the two strains, and controlling the temperature, the pH value, the oxygen content and the air pressure value in the fermentation tank (200);
4) Fermentation culture: in the fermentation process, air is regularly introduced into the fermentation tank (200) through the air inlet pipe (409), meanwhile, nutrient solution is regularly supplemented into the fermentation tank (200), and bottling and discharging can be carried out after 3-7 days of culture.
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