CN206375894U - Cultivate the second order fermentation system of bacillus thuringiensis - Google Patents
Cultivate the second order fermentation system of bacillus thuringiensis Download PDFInfo
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- CN206375894U CN206375894U CN201720028757.0U CN201720028757U CN206375894U CN 206375894 U CN206375894 U CN 206375894U CN 201720028757 U CN201720028757 U CN 201720028757U CN 206375894 U CN206375894 U CN 206375894U
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- tank
- order fermentation
- filter
- grade fermemtation
- bacillus thuringiensis
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model belongs to biological fermentation equipment technical field, and in particular to a kind of bacillus thuringiensis second order fermentation system.Bacillus thuringiensis second order fermentation system, including the one grade fermemtation tank and second order fermentation tank being sequentially connected in series, it is characterized in that, the top of described one grade fermemtation tank is connected with seeding tank, the bottom of one grade fermemtation tank is connected with two discharge pipes, one discharge pipe is connected with second order fermentation tank, and another discharge pipe is connected with filter I, and described second order fermentation tank is connected with filter II.Using the fermentation system of the present utility model for carrying two filters, different products can be obtained according to fermentation different phase, with suitable for different purpose purposes.In addition, the utility model is provided with chuck outside fermentation tank, can be while fermentation to the material temperature control in fermentation tank, or condensation or heating；Shower nozzle is provided with inside fermentation tank, can be according to make-up water the need for specific.
Description
Technical field
The utility model belongs to biological fermentation equipment technical field, and in particular to a kind of bacillus thuringiensis second order fermentation
System.
Background technology
Microbial fermentation and the target product of cell culture mainly have thalline, intracellular product and the class material of extracellular products three.
Required biochemical substances are extracted from zymotic fluid and cell culture fluid, it is necessary first to pre-process, in order to separation of solid and liquid, make metabolism
The process that isolates and purifies of product subsequently is smoothed out.Its main cause has the following aspects：Zymotic fluid is generally suspension, viscosity
Greatly, it is non-Newtonian fluid, is difficult filtering, and required biochemical substances are often only distributed in liquid phase, could effectively purify.
Also, in some zymotic fluids, thalline self-dissolving, this three classes material of nucleic acid, protein and other organic stickums can cause filter
Liquid is muddy, and filtering velocity is extremely slow, it is necessary to tries to increase the particle diameter of suspension, sinking speed is improved, in favor of filtering.Target product
Concentration in zymotic fluid is generally relatively low, in addition, the complicated component of zymotic fluid, substantial amounts of mycelium, strain metabolin and residue
Culture medium can cause very big influence to extraction, so carry out appropriate pretreatment to zymotic fluid, thus separate cell, thalline and
Other suspended particulates, and remove part soluble impurity and change the strainability of zymotic fluid, it is that biochemical substances were isolated and purified
Indispensable step in filter.
It is therefore desirable to be selected according to the characteristic of fermented product, strain used and zymotic fluid.Most of fermented products are present
In zymotic fluid, minority is present in thalline, and the situation in zymotic fluid and thalline with the presence of product is also very common.Therefore,
Need according to purpose, realize separation of solid and liquid by centrifuging or filtering, it is transferred to liquid phase.
The production period of bacillus thuringiensis is laundering period, exponential phase, balance period and decline stage.Gemma, crystal
Come off generation in the balance period middle and later periods, finally from separation of fermentative broth gemma and crystal product.At present, have many on using two
The mode of individual continuous fermentation tank series connection controls dilution rate, and first tank is used for primary fermentation production thalline and used, second tank is used to produce
Gemma and crystal are used.More than fermentation system do not design different phase thalline, gemma, metabolin separation equipment, and thing
In reality, thalline, gemma, its purposes of metabolite and the purpose difference of different phase are, it is necessary to separate as different purposes.
Utility model content
In order to solve above-mentioned technical problem, the utility model can provide different purposes there is provided a kind of in different phase
The golden second order fermentation systems of the Su Yun of product, the system is respectively arranged with filter after one grade fermemtation and second order fermentation, with
Obtain different purpose products.
Bacillus thuringiensis second order fermentation system, including the one grade fermemtation tank and second order fermentation tank being sequentially connected in series, one-level
The top of fermentation tank is connected with seeding tank, and the bottom of one grade fermemtation tank is connected with two discharge pipes, a discharge pipe connection
There is second order fermentation tank, another discharge pipe is connected with filter I, and second order fermentation tank is connected with filter II.Filtering dress
Put be specially：It is made up of the screen pack and filter frame of multiple-layer overlapped, its advantage is that flow is big, and speed is fast, and viability carbon is more,
Occupy little space, efficiency high.Polypropylene filter, filter paper, filter cloth, special decarburization filter membrane etc. may be selected in filter material.
There is regulating valve in the top Jun You pretreatment chamber of filter I and filter II, pretreatment chamber.
There is chuck outside one grade fermemtation tank, water inlet is arranged at the bottom of chuck, and delivery port is arranged at top；The outside of second order fermentation tank
Also there is chuck, water inlet is arranged at the bottom of chuck, and delivery port is arranged at top.
There are agitating shaft and stirring blade in one grade fermemtation tank, agitating shaft is driven by motor；Also there is stirring in second order fermentation tank
Axle and stirring blade, agitating shaft are driven by motor.
Spray water inlet and shower nozzle are arranged at the top in one grade fermemtation tank；Top in second order fermentation tank have spray water inlet and
Shower nozzle.
Bactericidal unit is connected with the charging aperture of one grade fermemtation tank.
The beneficial effects of the utility model are, using the fermentation system of the present utility model for carrying two filters,
Different products can be obtained according to fermentation different phase, with suitable for different purpose purposes.In addition, the utility model is in hair
Chuck is provided with outside fermentation tank, can be while fermentation to the material temperature control in fermentation tank, or condensation or heating；In fermentation tank
Inside is provided with shower nozzle, can be according to make-up water the need for specific.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
In figure, 1- bactericidal units, the stirring lodicule of 2- one grade fermemtation tanks, the agitating shaft of 3- one grade fermemtation tanks, 4- seeds
Tank, the spray water inlet of 5- one grade fermemtation tanks, the shower nozzle of 6- one grade fermemtation tanks, 7- one grade fermemtation tanks, 8- one grade fermemtation tank chucks,
The discharging opening I of 9- one grade fermemtation tanks, the discharging opening II of 10- one grade fermemtation tanks, the screen pack of 11- filters I, 12- filterings
The agitator of device I, the stirring lodicule of 13- second order fermentation tanks, the agitating shaft of 14- second order fermentation tanks, the spray of 15- second order fermentation tanks
Head, the spray water inlet of 16- second order fermentation tanks, the chuck of 17- second order fermentation tanks, 18- second order fermentation tanks, 19- second order fermentation tanks
Discharging opening I, the discharging opening II of 20- second order fermentation tanks, the agitator of 21- filters II, the screen pack of 22- filters II
Plate, 23- filters I, 24- filters II, 25- fluid reservoirs, 26- valves, 27- pretreatment chamber I, the tune of 28- pretreatment chamber I
Save valve, 29- pretreatment chamber II, the regulating valve of 30- pretreatment chamber II.
Embodiment
Come to be further described the utility model with reference to the accompanying drawings and detailed description, so as to this area
Technical staff know more about the utility model, but the utility model is not limited with this.
Embodiment 1
Bacillus thuringiensis second order fermentation system, including bactericidal unit 1, one grade fermemtation tank 7 and two grades being sequentially connected in series
Fermentation tank 18；
The top of one grade fermemtation tank 7 is connected with seeding tank 4, and the bottom of one grade fermemtation tank 7 is connected with two discharging openings, respectively
For one grade fermemtation tank discharging opening I 9 and the discharging opening II 10 of one grade fermemtation tank, the two discharging openings are connected to discharge pipe,
One discharge pipe is connected with second order fermentation tank 18, and another discharge pipe is connected with filter I 23；It is all provided between each pipeline
Valve 26 is equipped with, the flow direction to control material；The top of filter I 23 has inside pretreatment chamber I 27, pretreatment chamber I 27
Having in agitator 12, pretreatment chamber I 27 has regulating valve 28, the flowing to control material；
The top of one grade fermemtation tank 7 has one grade fermemtation tank to spray water inlet 6 and shower nozzle 5, for being kept the skin wet in fermentation；
There is one grade fermemtation tank chuck 8 outside of one grade fermemtation tank 7；By the chuck 8, hot water can be passed through in fermentation tank
Material heating, or be passed through cooling water give material cooling；
There are agitating shaft 3 and stirring blade 2 in one grade fermemtation tank 7, agitating shaft 3 is driven by motor, starts motor, agitating shaft 3
Start to drive stirring blade 2 to rotate, so that the material in fermentation tank is evenly；
The internal structure of second order fermentation tank is close with one grade fermemtation tank internal structure, has agitating shaft in second order fermentation tank
14, blade 13 is stirred, there is chuck 17 outside, has discharging opening I 19 and discharging opening II 20 in the bottom of second order fermentation tank；Discharging opening I
19 are connected with filter II 24 by pipeline；Discharging opening II 20 is connected 25 with fluid reservoir.
Second order fermentation tank 18 is connected with filter II 24, and agitator 21, filter 22 are arranged at the top of filter 22
There are the screen pack and filter frame of multiple-layer overlapped in inside, and its advantage is that flow is big, and speed is fast, and viability carbon is more, occupies little space,
Efficiency high.Polypropylene filter, filter paper, filter cloth, special decarburization filter membrane etc. may be selected in filter material.
The top of filter II 24, which has inside pretreatment chamber II 29, pretreatment chamber II 29, agitator 21, pretreatment chamber
There is regulating valve 30 in II 29, the flowing to control material.
Strain operationally, is first inoculated with by bacillus thuringiensis second order fermentation system above, the suitable culture of selection
Base expands culture, then puts into seeding tank 4, the material that input sterilizes by bactericidal unit 1 in one grade fermemtation tank 7, by one
Level culture tank 7 culture a period of time, select according to specific circumstances by by one grade fermemtation material be passed through filter I 23 or
Person is to be passed through second order fermentation tank 18 to continue to ferment；If material is after second order fermentation, material is directly inputted fluid reservoir 25 by selection
Or it is passed through the filtration treatment of filter II 24.Material can according to circumstances be added and helped in filter I 23 or filter II 24
Filtering agent, and turn on agitator stirring, make material be well mixed faster, favourable carrying out with filter progress.
Claims (7)
1. bacillus thuringiensis second order fermentation system, including the one grade fermemtation tank and second order fermentation tank being sequentially connected in series, its feature
It is, the top of described one grade fermemtation tank is connected with seeding tank, and the bottom of one grade fermemtation tank is connected with two discharge pipes, one
Bar discharge pipe is connected with second order fermentation tank, and another discharge pipe is connected with filter I, described second order fermentation tank connection
There is filter II.
2. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described filter I
With the top Jun You pretreatment chamber of filter II, there is regulating valve in described pretreatment chamber.
3. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described one grade fermemtation tank
There is chuck outside, and water inlet is arranged at the bottom of chuck, and delivery port is arranged at top；Also there are chuck, chuck in the outside of the second order fermentation tank
Bottom have water inlet, delivery port is arranged at top.
4. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described one grade fermemtation tank
Inside there are agitating shaft and stirring blade, described agitating shaft is driven by motor；Also there is agitating shaft in described second order fermentation tank and stir
Blade is mixed, agitating shaft is driven by motor.
5. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described one grade fermemtation tank
Spray water inlet and shower nozzle are arranged at interior top；Spray water inlet and shower nozzle are arranged at the top in described second order fermentation tank.
6. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described one grade fermemtation tank
Charging aperture at be connected with bactericidal unit.
7. bacillus thuringiensis second order fermentation system as claimed in claim 1, it is characterised in that described filter I
There is filter with the inside of filter II.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720028757.0U CN206375894U (en) | 2017-01-11 | 2017-01-11 | Cultivate the second order fermentation system of bacillus thuringiensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720028757.0U CN206375894U (en) | 2017-01-11 | 2017-01-11 | Cultivate the second order fermentation system of bacillus thuringiensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206375894U true CN206375894U (en) | 2017-08-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720028757.0U Expired - Fee Related CN206375894U (en) | 2017-01-11 | 2017-01-11 | Cultivate the second order fermentation system of bacillus thuringiensis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112897690A (en) * | 2021-02-05 | 2021-06-04 | 诸城舜沃农业科技有限公司 | Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation |
-
2017
- 2017-01-11 CN CN201720028757.0U patent/CN206375894U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112897690A (en) * | 2021-02-05 | 2021-06-04 | 诸城舜沃农业科技有限公司 | Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170804 Termination date: 20200111 |