CN206375894U - Cultivate the second order fermentation system of bacillus thuringiensis - Google Patents
Cultivate the second order fermentation system of bacillus thuringiensis Download PDFInfo
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- 229940097012 bacillus thuringiensis Drugs 0.000 title claims abstract description 15
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Abstract
Description
技术领域technical field
本实用新型属于生物发酵设备技术领域，具体涉及一种苏云金芽孢杆菌二级发酵系统。The utility model belongs to the technical field of biological fermentation equipment, in particular to a secondary fermentation system of Bacillus thuringiensis.
背景技术Background technique
微生物发酵和细胞培养的目标产物主要有菌体，胞内产物和胞外产物三类物质。从发酵液和细胞培养液中提取所需的生化物质，首先需要预处理，以便于固液分离，使代谢产物后续的分离纯化工序顺利进行。其主要原因有以下几个方面：发酵液多为悬浮液，粘度大，为非牛顿型流体，不易过滤，而所需的生化物质往往只有分布在液相，才能有效的提纯。并且，在有些发酵液中，菌体自溶，核酸、蛋白质及其它有机粘性物质这三类物质会造成滤液混浊，滤速极慢，必须设法增大悬浮物的颗粒直径，提高沉降速度，以利于过滤。目标产物在发酵液中的浓度通常较低，此外，发酵液的成分复杂，大量的菌丝体、菌种代谢物和剩余培养基会对提取造成很大的影响，所以对发酵液进行适当的预处理，从而分离细胞、菌体和其它悬浮颗粒，并除去部分可溶性杂质和改变发酵液的过滤性能，是生化物质分离纯化过滤中不可少的步骤。The target products of microbial fermentation and cell culture mainly include bacteria, intracellular products and extracellular products. To extract the required biochemical substances from fermentation broth and cell culture fluid, pretreatment is first required to facilitate solid-liquid separation, so that the subsequent separation and purification process of metabolites can proceed smoothly. The main reasons are as follows: the fermentation broth is mostly a suspension with high viscosity, which is a non-Newtonian fluid and is not easy to filter, and the required biochemical substances are often only distributed in the liquid phase in order to be effectively purified. Moreover, in some fermentation broths, the three types of substances such as bacterial autolysis, nucleic acid, protein and other organic viscous substances will cause the filtrate to be turbid, and the filtration rate is extremely slow. It is necessary to try to increase the particle diameter of the suspended solids and increase the settling velocity. Good for filtering. The concentration of the target product in the fermentation broth is usually low. In addition, the composition of the fermentation broth is complex, and a large amount of mycelium, strain metabolites and remaining medium will have a great impact on the extraction, so proper fermentation of the fermentation broth Pretreatment, so as to separate cells, bacteria and other suspended particles, remove some soluble impurities and change the filtration performance of fermentation broth, is an indispensable step in the separation, purification and filtration of biochemical substances.
所以，需要根据发酵产品、所用菌种和发酵液的特性来选择。大多数发酵产品存在于发酵液中，少数存在于菌体中，而发酵液和菌体中都有产物存在的情况也很常见。因此，需要根据目的，通过离心或过滤来实现固液分离，使其转入液相。Therefore, it needs to be selected according to the characteristics of the fermented product, the strain used and the fermented liquid. Most of the fermentation products exist in the fermentation broth, a few exist in the cells, and it is not uncommon for products to exist in both the fermentation broth and the cells. Therefore, solid-liquid separation needs to be achieved by centrifugation or filtration according to the purpose, so that it can be transferred to the liquid phase.
苏云金芽孢杆菌的生产期为适应期、对数生长期、平衡期和衰亡阶段。芽孢、晶体的脱落产生在平衡期中后期，最后从发酵液分离芽孢和晶体产品。目前，有不少关于采用两个连续发酵罐串联的方式来控制稀释率，第一个罐用于前发酵产菌体用，第二个罐用于产芽孢和晶体用。以上的发酵系统并未设计不同阶段的菌体、芽孢、代谢物的分离设备，而事实上，不同阶段的菌体、芽孢、代谢产物其用途和目的均不同，需要分离作为不同的用途。The production period of Bacillus thuringiensis includes adaptation period, logarithmic growth period, equilibrium period and decay period. The shedding of spores and crystals occurs in the middle and late stages of the equilibrium period, and the spores and crystal products are finally separated from the fermentation broth. At present, there are many discussions on the use of two continuous fermentation tanks in series to control the dilution rate. The first tank is used for pre-fermentation to produce bacteria, and the second tank is used to produce spores and crystals. The above fermentation system does not design separation equipment for bacteria, spores, and metabolites at different stages. In fact, bacteria, spores, and metabolites at different stages have different uses and purposes, and they need to be separated for different purposes.
实用新型内容Utility model content
为了解决上述的技术问题，本实用新型提供了一种能在不同阶段提供不同用途的产物的苏云金二级发酵系统，该系统在一级发酵和二级发酵之后，分别设置有过滤装置，以得到不同的目的产物。In order to solve the above-mentioned technical problems, the utility model provides a secondary fermentation system of thuringiensis that can provide products of different uses at different stages. After the primary fermentation and secondary fermentation, the system is respectively equipped with filtering devices to obtain different purpose products.
苏云金芽孢杆菌二级发酵系统，包括依次串联的一级发酵罐和二级发酵罐，一级发酵罐的上部连接有种子罐，一级发酵罐的下部连接有两条出料管道，一条出料管道连接有二级发酵罐，另一条出料管道连接有过滤装置Ⅰ，二级发酵罐连接有过滤装置Ⅱ。过滤装置的具体为：由多层重叠的过滤网板和过滤框组成，其优点是流量大，速度快，存活性碳多，占用空间小，效率高。滤材可选择聚丙烯滤膜、滤纸、滤布、专用脱碳过滤膜等。The secondary fermentation system of Bacillus thuringiensis consists of a primary fermentation tank and a secondary fermentation tank connected in series in sequence. The upper part of the primary fermentation tank is connected with a seed tank, and the lower part of the primary fermentation tank is connected with two discharge pipes, one for discharge The pipeline is connected with a secondary fermentation tank, the other discharge pipeline is connected with a filtering device I, and the secondary fermentation tank is connected with a filtering device II. The details of the filter device are: it is composed of multi-layered filter screen plates and filter frames. Its advantages are large flow, fast speed, more survivable carbon, small space occupation and high efficiency. The filter material can choose polypropylene filter membrane, filter paper, filter cloth, special decarbonization filter membrane, etc.
过滤装置Ⅰ和过滤装置Ⅱ的上部均有预处理室，预处理室内均有调节阀。There are pretreatment chambers on the upper part of the filter device I and the filter device II, and there are regulating valves in the pretreatment chambers.
一级发酵罐外部有夹套，夹套的下部有进水口，上部有出水口；二级发酵罐的外部也有夹套，夹套的下部有进水口，上部有出水口。There is a jacket on the outside of the first-stage fermentation tank, the lower part of the jacket has a water inlet, and the upper part has a water outlet; the second-stage fermentation tank also has a jacket, the lower part of the jacket has a water inlet, and the upper part has a water outlet.
一级发酵罐内有搅拌轴和搅拌桨片，搅拌轴由电机带动；二级发酵罐内也有搅拌轴和搅拌桨片，搅拌轴由电机带动。There are stirring shafts and stirring paddles in the primary fermentation tank, and the stirring shaft is driven by a motor; there are also stirring shafts and stirring paddles in the secondary fermentation tank, and the stirring shaft is driven by a motor.
一级发酵罐内的上部有喷淋水进口及喷头；二级发酵罐内的上部有喷淋水进口及喷头。The upper part of the first-stage fermentation tank has a spray water inlet and a nozzle; the upper part of the second-stage fermentation tank has a spray water inlet and a nozzle.
一级发酵罐的进料口处连接有灭菌装置。A sterilizing device is connected to the feed port of the primary fermentation tank.
本实用新型的有益效果在于，采用本实用新型的带有两个过滤装置的发酵系统，可以根据发酵不同阶段获得不同的产物，以适用于不同的目的用途。另外，本实用新型在发酵罐外部设置了夹套，可以在发酵的同时对发酵罐内的物料温控，或冷凝或加热；在发酵罐内部设置了喷头，可以根据具体的需要补给水。The beneficial effect of the utility model is that, by adopting the fermentation system with two filtering devices of the utility model, different products can be obtained according to different stages of fermentation, so as to be suitable for different purposes. In addition, the utility model is equipped with a jacket outside the fermentation tank, which can control the temperature of the material in the fermentation tank, or condense or heat it while fermenting; a nozzle is installed inside the fermentation tank to supply water according to specific needs.
附图说明Description of drawings
图1为本实用新型的结构示意图；Fig. 1 is the structural representation of the utility model;
图中，1-灭菌装置，2-一级发酵罐的搅拌浆片， 3-一级发酵罐的搅拌轴，4-种子罐，5-一级发酵罐的喷淋水进口，6-一级发酵罐的喷头，7-一级发酵罐，8-一级发酵罐夹套，9-一级发酵罐的出料口Ⅰ，10-一级发酵罐的出料口Ⅱ，11-过滤装置Ⅰ的过滤网板，12-过滤装置Ⅰ的搅拌器，13-二级发酵罐的搅拌浆片，14-二级发酵罐的搅拌轴，15-二级发酵罐的喷头，16-二级发酵罐的喷淋水进口，17-二级发酵罐的夹套，18-二级发酵罐，19-二级发酵罐的出料口Ⅰ，20-二级发酵罐的出料口Ⅱ，21-过滤装置Ⅱ的搅拌器，22-过滤装置Ⅱ的过滤网板，23-过滤装置Ⅰ，24-过滤装置Ⅱ，25-储液罐，26-阀门，27-预处理室Ⅰ，28-预处理室Ⅰ的调节阀，29-预处理室Ⅱ，30-预处理室Ⅱ的调节阀。In the figure, 1-sterilization device, 2-stirring blade of the first-stage fermenter, 3-stirrer shaft of the first-stage fermenter, 4-seed tank, 5-spray water inlet of the first-stage fermenter, 6-a The nozzle of the first-stage fermenter, 7-the first-stage fermenter, 8-the jacket of the first-stage fermenter, 9-the outlet Ⅰ of the first-stage fermenter, 10-the outlet II of the first-stage fermenter, 11-the filter device Ⅰ filter plate, 12- the agitator of the filter device Ⅰ, 13- the stirring blade of the secondary fermentation tank, 14- the stirring shaft of the secondary fermentation tank, 15- the nozzle of the secondary fermentation tank, 16- the secondary fermentation Spray water inlet of the tank, 17-jacket of the secondary fermentation tank, 18-secondary fermentation tank, 19-discharge port Ⅰ of the secondary fermentation tank, 20-discharge port II of the secondary fermentation tank, 21- Stirrer of filter device II, 22-filter screen plate of filter device II, 23-filter device I, 24-filter device II, 25-liquid storage tank, 26-valve, 27-pretreatment chamber I, 28-pretreatment Regulating valve of chamber I, 29-pretreatment chamber II, 30-regulating valve of pretreatment chamber II.
具体实施方式detailed description
下面结合附图和具体实施方式来对本实用新型作更进一步的说明，以便本领域的技术人员更了解本实用新型，但并不以此限制本实用新型。 The utility model will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the utility model, but the utility model is not limited thereto.
实施例1Example 1
苏云金芽孢杆菌二级发酵系统，包括依次串联的灭菌装置1、一级发酵罐7和二级发酵罐18；The secondary fermentation system for Bacillus thuringiensis comprises a sterilizing device 1, a primary fermentation tank 7 and a secondary fermentation tank 18 connected in series in sequence;
一级发酵罐7的顶部连接有种子罐4，一级发酵罐7的下部连接有两个出料口，分别为一级发酵罐出料口Ⅰ9和一级发酵罐的出料口Ⅱ10，这两个出料口分别连接有出料管道，一条出料管道连接有二级发酵罐18，另一条出料管道连接有过滤装置Ⅰ23；各管道之间均设置有阀门26，用以控制物料的流向；过滤装置Ⅰ23的上部有预处理室Ⅰ27，预处理室Ⅰ27内部有搅拌器12，预处理室Ⅰ27内有调节阀28，用以控制物料的流动；The top of the primary fermentation tank 7 is connected with the seed tank 4, and the bottom of the primary fermentation tank 7 is connected with two outlets, which are respectively the discharge port I9 of the primary fermentation tank and the discharge port II10 of the primary fermentation tank. The two outlets are respectively connected with discharge pipelines, one discharge pipeline is connected with the secondary fermentation tank 18, and the other discharge pipeline is connected with the filter device I23; valves 26 are arranged between each pipeline to control the flow of materials. Flow direction: there is a pretreatment chamber I27 on the upper part of the filter device I23, there is an agitator 12 inside the pretreatment chamber I27, and there is a regulating valve 28 in the pretreatment chamber I27 to control the flow of materials;
一级发酵罐7的上部有一级发酵罐喷淋水进口6和喷头5，用于在发酵时补充水分；The upper part of the first-stage fermenter 7 has a first-stage fermenter spray water inlet 6 and a nozzle 5 for replenishing water during fermentation;
一级发酵罐7的外部有一级发酵罐夹套8；通过该夹套8，可以通入热水给发酵罐内的物料加温，或者是通入冷却水给物料降温；There is a first-stage fermenter jacket 8 outside the first-stage fermenter 7; through the jacket 8, hot water can be introduced to heat the material in the fermenter, or cooling water can be introduced to cool the material;
一级发酵罐7内有搅拌轴3和搅拌桨片2，搅拌轴3由电机带动，启动电机，搅拌轴3开始带动搅拌桨片2转动，从而使发酵罐内的物料更均匀；The first-stage fermentation tank 7 has a stirring shaft 3 and a stirring paddle 2, the stirring shaft 3 is driven by a motor, the motor is started, and the stirring shaft 3 starts to drive the stirring paddle 2 to rotate, so that the materials in the fermentation tank are more uniform;
二级发酵罐的内部结构与一级发酵罐内部结构相接近，二级发酵罐内有搅拌轴14，搅拌桨片13，外部有夹套17，在二级发酵罐的下部有出料口Ⅰ19和出料口Ⅱ20；出料口Ⅰ19与过滤装置Ⅱ24通过管道相连接；出料口Ⅱ20与储液罐相连接25。The internal structure of the secondary fermentation tank is similar to the internal structure of the primary fermentation tank. There is a stirring shaft 14 inside the secondary fermentation tank, a stirring paddle 13, a jacket 17 outside, and a discharge port I19 at the lower part of the secondary fermentation tank. and the discharge port II20; the discharge port I19 is connected with the filter device II24 through pipelines; the discharge port II20 is connected with the liquid storage tank 25.
二级发酵罐18连接有过滤装置Ⅱ24，过滤装置22的上部有搅拌器21，过滤装置22内部有多层重叠的过滤网板和过滤框，其优点是流量大，速度快，存活性碳多，占用空间小，效率高。滤材可选择聚丙烯滤膜、滤纸、滤布、专用脱碳过滤膜等。The secondary fermentation tank 18 is connected with a filter device II 24, and the upper part of the filter device 22 is provided with an agitator 21. There are multiple layers of overlapping filter screen plates and filter frames inside the filter device 22. , small footprint and high efficiency. The filter material can choose polypropylene filter membrane, filter paper, filter cloth, special decarbonization filter membrane, etc.
过滤装置Ⅱ24的上部有预处理室Ⅱ29，预处理室Ⅱ29内部有搅拌器21，预处理室Ⅱ29内有调节阀30，用以控制物料的流动。There is a pretreatment chamber II29 on the upper part of the filter device II24, and there is an agitator 21 inside the pretreatment chamber II29, and a regulating valve 30 is arranged in the pretreatment chamber II29 to control the flow of materials.
以上的苏云金芽孢杆菌二级发酵系统在运行时，先将菌种接种，选择合适的培养基扩大培养，然后投入种子罐4，在一级发酵罐7内投入经过灭菌装置1灭菌的物料，经过一级培养罐7培养一段时间，根据具体的情况选择将经过一级发酵的物料通入过滤装置Ⅰ23或者是通入二级发酵罐18继续发酵；若物料经过二级发酵后，选择将物料直接输入储液罐25或通入过滤装置Ⅱ24过滤处理。物料在过滤装置Ⅰ23或过滤装置Ⅱ24时，可根据情况加入助滤剂，并开启搅拌器搅拌，使物料更快的混合均匀，有利用过滤工序的进行。When the above Bacillus thuringiensis secondary fermentation system is in operation, first inoculate the bacteria, select a suitable medium to expand the cultivation, then put it into the seed tank 4, and put the material sterilized by the sterilization device 1 into the primary fermentation tank 7 After being cultivated for a period of time in the primary culture tank 7, according to the specific situation, the material that has undergone the primary fermentation is selected to pass into the filter device I23 or the secondary fermentation tank 18 to continue fermentation; if the material is passed through the secondary fermentation, the selection will The material is directly input into the liquid storage tank 25 or passed into the filter device II 24 for filtration treatment. When the material is in the filter device I23 or filter device II24, filter aid can be added according to the situation, and the agitator is turned on to stir the material, so that the material can be mixed faster and evenly, and the filter process can be carried out effectively.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112897690A (en) * | 2021-02-05 | 2021-06-04 | 诸城舜沃农业科技有限公司 | Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation |
| CN115011445A (en) * | 2022-06-08 | 2022-09-06 | 广州汉方医学生物科技有限公司 | Extracellular polysaccharide fermentation extraction device and fermentation extraction method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112897690A (en) * | 2021-02-05 | 2021-06-04 | 诸城舜沃农业科技有限公司 | Anaerobic fermentation device capable of being switched randomly based on one-stage two-stage fermentation |
| CN115011445A (en) * | 2022-06-08 | 2022-09-06 | 广州汉方医学生物科技有限公司 | Extracellular polysaccharide fermentation extraction device and fermentation extraction method |
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