CN109593644A - A kind of device for Halophiles induction carbonate mineral - Google Patents
A kind of device for Halophiles induction carbonate mineral Download PDFInfo
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- CN109593644A CN109593644A CN201910123633.4A CN201910123633A CN109593644A CN 109593644 A CN109593644 A CN 109593644A CN 201910123633 A CN201910123633 A CN 201910123633A CN 109593644 A CN109593644 A CN 109593644A
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- reaction chamber
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
The present invention provides a kind of devices for Halophiles induction carbonate mineral, including sampling device and several reaction members；Sampling device includes total injection port for launching medium liquid and several split sampling mouths being connected with total injection port；Reaction member includes that reaction chamber, filter for installation, pH regulating device and temperature control agitating device, pH regulating device are set to the top of reaction chamber, including acid solution tank and alkali liquid tank, controls the pH value of reaction chamber by adjusting the liquid inlet volume of the two；Temperature control agitating device includes blender and the heater for adjusting and controlling temperature, to stir and heat to the indoor reaction solution of reaction；Filter for installation is removable installed in the bottom end of reaction chamber；Reaction chamber is equipped with Multifunctional sample injection mouth, for controlling experimental group variable；Medium liquid is put by total injection port, is flowed by split sampling mouth in the reaction chamber of differential responses unit, is controlled by Multifunctional sample injection mouth experimental group variable.
Description
Technical field
The present invention relates to carbonate rock ensaying technical fields, and in particular to one kind induces carbonate mine for Halophiles
The device of object.
Background technique
In recent years, due to the extensive use of microbial mineralization, obtaining mineral using microorganism induction becomes the research of people
Hot spot.Halophiles is the important monoid in extreme microorganism, due to special with unique physiology, biochemistry and cytology etc.
Sign, current and future all have important researching value in fields such as environment, chemical industry and biotechnologies, thus by the country
The outer more and more extensive concerns of scholar.A kind of device of Halophiles induction carbonate mineral is invented in research, is able to solve resistance to height
Salt, fluid Mass and heat transfer and mineral product efficiently separate and the key technology difficulties such as analysis, is that the carbon sequestration mineralising of Halophiles converts
The relevant environment of process, learn and biological study provides important intellectual property support.
However, in Halophiles induction mineral under carrying out different salinity, different magnesium calcium sources, different magnesium calcium ratios, conventional procedure
Extremely cumbersome, process flow is excessively complicated.
Summary of the invention
In view of the above-mentioned problems in the prior art, the present invention provides it is a kind of for Halophiles induction carbonate mineral device,
Equipped with total injection port and several reaction members, induces multiple reaction members of mineral reaction in parallel, differential responses unit is set,
Achievable total can carry out experimental group experiment simultaneously.
The following technical solution is employed by the present invention:
A kind of device for Halophiles induction carbonate mineral, including sampling device and several reaction members；It is described
Sampling device includes total injection port for launching medium liquid and several split sampling mouths being connected with total injection port；Institute
Stating reaction member includes that reaction chamber, filter for installation, pH regulating device and temperature control agitating device, the pH regulating device are set
It is placed in the top of the reaction chamber, including acid solution tank and alkali liquid tank, is controlled by adjusting the liquid inlet volume of the two anti-in reaction chamber
Answer the pH value of liquid；The temperature control agitating device includes blender and the heater for adjusting and controlling temperature, to reaction
Indoor reaction solution stirs and heats；The filter for installation is removable installed in the bottom end of reaction chamber；On reaction chamber
Equipped with Multifunctional sample injection mouth, for controlling experimental group variable.
In above scheme, medium liquid is put by total injection port, flows into differential responses unit by split sampling mouth
In reaction chamber, experimental group variable is controlled by Multifunctional sample injection mouth.
It induces multiple reaction members of mineral reaction in parallel, differential responses unit is set, it can be achieved that total can be simultaneously
Carry out experimental group experiment.
Halophiles induces carbonate mineral under different salinity, different magnesium calcium sources, different magnesium calcium ratios in reaction chamber, passes through
The mineral filter device of the separable disassembly in lower section, is separated by filtration carbonate mineral precipitating, and analysis measures its ingredient and phase character；
Temperature controls agitating device, stirs and evenly mixs fluid nutrient medium, is in 37 DEG C using heater control reaction chamber temperature.
Total injection port is mainly used for the injection of NaCl, Na2CO3, NaHCO3 solution and Halophiles bacterium solution.Multifunctional sample injection mouth
It is mainly used for controlling experimental group variable, facilitates setting different salinity, different magnesium calcium sources, different magnesium calcium ratios, induce carbonate mine
Object.
Preferably, the split sampling mouth is split sampling mouth with a scale, for accurately controlling culture medium each component
Dosage.
Preferably, the lower end of the reaction chamber is to be designed using reverse frustoconic.Conducive to the collection of mineral precipitation.
Preferably, the material of the reaction chamber uses height Salt-tolerant Materials polypropylene.Can high pressure sterilization, easy to disassemble group
Dress.
Preferably, the filter for installation is set to the bottom end of reaction chamber and is detachably connected as being threadedly coupled.Filter
Device is designed as the filter of the detachable assembling of screw thread, and carbonate mineral precipitating is extracted in special filtering, to facilitate mineral extraction point
Analysis.
The invention has the advantages that:
Different salinity, magnesium calcium source and magnesium calcium connect operation than induction mineral and carbonate mineral extraction process integration
It is continuous to carry out, time and energy is greatly saved, Halophiles is made to induce mine under different salinity, different magnesium calcium sources, different magnesium calcium ratios
It is more quick when object, it is more convenient to be separated by filtration carbonate mineral precipitating；
Each junction leakproofness is good, prevents microbiological contamination；
Package unit uses height Salt-tolerant Materials polypropylene, can high pressure sterilization, assembling easy to disassemble greatly improves work
Make efficiency；
Setting one quantitative launches switchs, and quantitatively launches different amounts of salt and magnesium calcium solution, and more convenient setting is different
The culture medium of gradient；
Separation and subsequent characterizations in time are needed to final carbonate mineral precipitating, a detachable assembling is arranged in each unit
Filter device, carbonate mineral precipitating is extracted in special filtering, and to facilitate mineral to extract, more easily measurement carbonate mineral is heavy
It forms sediment.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that the device of carbonate mineral is induced for Halophiles.
Wherein, 1 is total injection port, and 2 be Multifunctional sample injection mouth, and 3 be pH regulating device, and 4 be heater, and 5 be with a scale
Split sampling mouth, 6 be blender, and 7 be filter membrane, and 8 be filter.
Specific embodiment
The present invention is specifically described with reference to the accompanying drawing:
In conjunction with Fig. 1, a kind of device for Halophiles induction carbonate mineral, including sampling device and several reaction lists
Member；The sampling device include total injection port for launching medium liquid and be connected with total injection port several be diverted into
Sample mouth；The reaction member includes reaction chamber, filter for installation, pH regulating device and temperature control agitating device, the pH tune
Regulating device is set to the top of the reaction chamber, including acid solution tank and alkali liquid tank, is controlled by adjusting the liquid inlet volume of the two anti-
Answer the pH value of indoor reaction solution；The temperature control agitating device includes blender and the heater for adjusting and controlling temperature, is used
To be stirred and heated to the indoor reaction solution of reaction；The filter for installation is removable installed in the bottom end of reaction chamber；
The reaction chamber is equipped with Multifunctional sample injection mouth.
The split sampling mouth is split sampling mouth with a scale, for accurately controlling the dosage of culture medium each component；
The lower end of the reaction chamber is to be designed using reverse frustoconic；The material of the reaction chamber uses height Salt-tolerant Materials polypropylene；Institute
It states filter for installation and is set to the bottom end of reaction chamber and be detachably connected as being threadedly coupled, be arranged on the filter of filter for installation
There is filter membrane, filter for installation is designed as the filter of the detachable assembling of screw thread, and carbonate mineral precipitating is extracted in special filtering, with side
Just mineral extraction and analysis.
Medium liquid is put by total injection port, is flowed into the reaction chamber of differential responses unit by split sampling mouth, is led to
Multi-functional injection port is crossed to control experimental group variable.
Embodiment 1
Control different magnesium sources:
After the device is carried out high pressure sterilization, blender 6, the same calcium source (CaCl of fluid nutrient medium are opened2·2H2O), salt
It spends (15%), fluid nutrient medium (is contained into CaCl2·2H2O it) is put into, passes through according to 0,3,6,9,12 ratio more from total injection port 1
Function injection port 2 is separately added into required MgCl2·6H2O, after mixing evenly high pressure sterilization.By total injection port, to each anti-
Unit is answered to be injected separately into 1mol/LNa2CO3、NaHCO3Solution surveys its pH with pH regulating device 3 after stirring evenly, passes through adjusting
The liquid inlet volume of acid solution tank and alkali liquid tank controls the pH value of reaction chamber, makes pH stable 7 or so.According to chamber volume
10% adds Halophiles bacterium solution from total injection port 1, and setting temperature controls 37 DEG C of agitating device temperature, successive reaction 14 days.It opens
Filter device switch, fluid nutrient medium outflow, carbonate mineral precipitating are obtained by filtration by filter membrane 7, and rotation screw thread is detachable
Filter 8 collects carbonate mineral precipitating, carries out mineral product phenetic analysis.
When the different magnesium source of experiment control, fluid nutrient medium is consistent with the above, same calcium source (CaCl2·2H2O), salinity
(15%), fluid nutrient medium (is contained into CaCl2·2H2O it) is put into from total injection port 1, passes through more function according to 0,3,6,9,12 ratio
Energy injection port 2 is separately added into required MgSO4·7H2O also embodies the Multifunctional sample injection mouth and is mainly used for control experiment
Group variable.Carry out operation same as described above.High pressure sterilization after mixing evenly.Total 1 filter of injection port is opened, is injected separately into
1mol/LNa2CO3、NaHCO3Solution surveys its pH with pH regulating device 3 after stirring evenly, by adjusting acid solution tank and alkali liquid tank
Liquid inlet volume control the pH value of reaction chamber, make pH stable 7 or so.According to chamber volume 10% from total injection port 1
Halophiles bacterium solution is added, setting temperature controls 37 DEG C of agitating device temperature, successive reaction 14 days.Open filter device switch, liquid
The outflow of body culture medium, carbonate mineral precipitate logical filter membrane 7 and are obtained by filtration, and rotate screw thread detachable filter 8, collect carbonate
Mineral precipitation carries out mineral product phenetic analysis.
Embodiment 2
Control different calcium sources:
After the device is carried out high pressure sterilization, blender 6, the same magnesium source (MgSO of fluid nutrient medium are opened4·7H2O), salt
It spends (15%), fluid nutrient medium (is contained into MgSO4·7H2O it) is put into, passes through according to 0,3,6,9,12 ratio more from total injection port 1
Function injection port 2 is separately added into required C4CaH6O4·H2O carries out operation same as described above.High pressure sterilization after mixing evenly.
By total injection port, 1mol/LNa is injected separately into each reaction member2CO3、NaHCO3Solution, with pH tune after stirring evenly
Regulating device 3 surveys its pH, controls the pH value of reaction chamber by adjusting the liquid inlet volume of acid solution tank and alkali liquid tank, makes pH stable 7
Left and right.Halophiles bacterium solution is added from total injection port 1 according to the 10% of chamber volume, setting temperature controls agitating device temperature 37
DEG C, successive reaction 14 days.Filter device switch, fluid nutrient medium outflow are opened, carbonate mineral precipitating is filtered by filter membrane 7
It obtains, rotates screw thread detachable filter 8, collect carbonate mineral precipitating, carry out mineral product phenetic analysis.
Embodiment 3
Control different salinity:
After the device is carried out high pressure sterilization, blender 6, the same calcium source (CaCl of fluid nutrient medium are opened2·2H2O), same
One magnesium source (MgSO4·7H2O), fluid nutrient medium (is contained into CaCl2·2H2O、MgSO4·7H2O it) is put into from total injection port 1, according to
0,3,6,9,12 ratio is separately added into required MgSO by Multifunctional sample injection mouth 24·7H2O.High pressure sterilization after mixing evenly.
By total injection port, 1mol/LNa is injected separately into each reaction member2CO3、NaHCO3Solution, with pH tune after stirring evenly
Regulating device 3 surveys its pH, controls the pH value of reaction chamber by adjusting the liquid inlet volume of acid solution tank and alkali liquid tank, makes pH stable 7
Left and right.Halophiles bacterium solution is added from total injection port 1 according to the 10% of chamber volume, setting temperature controls agitating device temperature 37
DEG C, successive reaction 14 days.Filter device switch, fluid nutrient medium outflow are opened, carbonate mineral precipitates logical filter membrane 7 and filtered
It arrives, rotates screw thread detachable filter 8, collect carbonate mineral precipitating, carry out mineral product phenetic analysis.
Required NaCl is added in liquid medium according to 5%, 10%, 15%, 20%, 25% salinity.It carries out identical
Operation repeats above-mentioned experiment five time.
Certainly, the above description is not a limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The variations, modifications, additions or substitutions that the technical staff in domain is made within the essential scope of the present invention also should belong to of the invention
Protection scope.
Claims (5)
1. a kind of device for Halophiles induction carbonate mineral, which is characterized in that including sampling device and several reactions
Unit；The sampling device includes total injection port for launching medium liquid and several shuntings being connected with total injection port
Injection port；The reaction member includes reaction chamber, filter for installation, pH regulating device and temperature control agitating device, the pH
Regulating device is set to the top of the reaction chamber, including acid solution tank and alkali liquid tank, is controlled by adjusting the liquid inlet volume of the two
The pH value of reaction solution in reaction chamber；The temperature controls the heater that agitating device includes blender with adjusts and control temperature,
To be stirred and heated to the indoor reaction solution of reaction；The filter for installation is removable installed in the bottom of reaction chamber
End；Reaction chamber is equipped with Multifunctional sample injection mouth, for controlling experimental group variable.
2. a kind of device for Halophiles induction carbonate mineral according to claim 1, which is characterized in that described point
Stream injection port is split sampling mouth with a scale, for accurately controlling the dosage of culture medium each component.
3. a kind of device for Halophiles induction carbonate mineral according to claim 1, which is characterized in that described anti-
The lower end for answering room is to be designed using reverse frustoconic.
4. a kind of device for Halophiles induction carbonate mineral according to claim 1 or 3, which is characterized in that institute
It states filter for installation and is set to the bottom end of reaction chamber and be detachably connected as being threadedly coupled.
5. a kind of device for Halophiles induction carbonate mineral according to claim 1 or 3, which is characterized in that institute
The material of reaction chamber is stated using height Salt-tolerant Materials polypropylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910123633.4A CN109593644A (en) | 2019-02-19 | 2019-02-19 | A kind of device for Halophiles induction carbonate mineral |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910123633.4A CN109593644A (en) | 2019-02-19 | 2019-02-19 | A kind of device for Halophiles induction carbonate mineral |
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| Publication Number | Publication Date |
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| CN109593644A true CN109593644A (en) | 2019-04-09 |
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| CN201910123633.4A Withdrawn CN109593644A (en) | 2019-02-19 | 2019-02-19 | A kind of device for Halophiles induction carbonate mineral |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070479A (en) * | 2013-12-16 | 2015-06-25 | 연세대학교 산학협력단 | Reactor for inorganic carbonation |
| CN106018751A (en) * | 2016-07-01 | 2016-10-12 | 山东科技大学 | Carbonate mineral dolomitization simulation study system and application method thereof |
| US20170182458A1 (en) * | 2015-12-29 | 2017-06-29 | Yuanchu Technology (Beijing) Co., Ltd. | Apparatus and method for absorbing and mineralizing carbon dioxide |
| CN108395983A (en) * | 2018-01-12 | 2018-08-14 | 山东科技大学 | A kind of configuration of culture medium and mineral obtain system |
| CN208279358U (en) * | 2018-01-25 | 2018-12-25 | 嘉兴市欣欣仪器设备有限公司 | Laboratory experiment use in waste water treatment coagulation tank |
| CN209685782U (en) * | 2019-02-19 | 2019-11-26 | 山东科技大学 | A kind of device for Halophiles induction carbonate mineral |
-
2019
- 2019-02-19 CN CN201910123633.4A patent/CN109593644A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070479A (en) * | 2013-12-16 | 2015-06-25 | 연세대학교 산학협력단 | Reactor for inorganic carbonation |
| US20170182458A1 (en) * | 2015-12-29 | 2017-06-29 | Yuanchu Technology (Beijing) Co., Ltd. | Apparatus and method for absorbing and mineralizing carbon dioxide |
| CN106018751A (en) * | 2016-07-01 | 2016-10-12 | 山东科技大学 | Carbonate mineral dolomitization simulation study system and application method thereof |
| CN108395983A (en) * | 2018-01-12 | 2018-08-14 | 山东科技大学 | A kind of configuration of culture medium and mineral obtain system |
| CN208279358U (en) * | 2018-01-25 | 2018-12-25 | 嘉兴市欣欣仪器设备有限公司 | Laboratory experiment use in waste water treatment coagulation tank |
| CN209685782U (en) * | 2019-02-19 | 2019-11-26 | 山东科技大学 | A kind of device for Halophiles induction carbonate mineral |
Non-Patent Citations (1)
| Title |
|---|
| 李福春;郭文文;: "三种好氧细菌诱导碳酸钙矿物的形成", 南京大学学报(自然科学版), no. 06, pages 665 - 672 * |
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Application publication date: 20190409 |