CN109593644A - A kind of device for Halophiles induction carbonate mineral - Google Patents
A kind of device for Halophiles induction carbonate mineral Download PDFInfo
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- 229910001748 carbonate mineral Inorganic materials 0.000 title claims abstract description 30
- 230000006698 induction Effects 0.000 title description 9
- 238000006243 chemical reaction Methods 0.000 claims abstract description 66
- 238000002347 injection Methods 0.000 claims abstract description 41
- 239000007924 injection Substances 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 239000002253 acid Substances 0.000 claims abstract description 8
- 238000010979 pH adjustment Methods 0.000 claims abstract 4
- 230000001939 inductive effect Effects 0.000 claims abstract 3
- 239000000243 solution Substances 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000004743 Polypropylene Substances 0.000 claims description 4
- -1 polypropylene Polymers 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 239000003513 alkali Substances 0.000 abstract description 7
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 17
- 229910052500 inorganic mineral Inorganic materials 0.000 description 15
- 239000011707 mineral Substances 0.000 description 15
- 235000010755 mineral Nutrition 0.000 description 15
- 239000012530 fluid Substances 0.000 description 13
- 235000015097 nutrients Nutrition 0.000 description 12
- 238000005070 sampling Methods 0.000 description 12
- ZFXVRMSLJDYJCH-UHFFFAOYSA-N calcium magnesium Chemical compound [Mg].[Ca] ZFXVRMSLJDYJCH-UHFFFAOYSA-N 0.000 description 11
- 230000001376 precipitating effect Effects 0.000 description 11
- 230000001105 regulatory effect Effects 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 238000009434 installation Methods 0.000 description 8
- 238000001914 filtration Methods 0.000 description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 235000002639 sodium chloride Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000001110 calcium chloride Substances 0.000 description 4
- 229910001628 calcium chloride Inorganic materials 0.000 description 4
- 229910052564 epsomite Inorganic materials 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 229910052749 magnesium Inorganic materials 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000009919 sequestration Effects 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
本发明提供了一种用于嗜盐菌诱导碳酸盐矿物的装置，包括进样装置和若干个反应单元；进样装置包括用于投放培养基液体的总进样口和与总进样口相连的若干个分流进样口；反应单元包括反应室、过滤器装置、pH调节装置和温度控制搅拌装置，pH调节装置设置于反应室的顶端，包括酸液罐和碱液罐，通过调节二者的进液量来控制反应室的pH值；温度控制搅拌装置包括搅拌器和调节并控制温度的加热器，用以对反应室内的反应液进行搅拌和加热；过滤器装置可拆卸地设置于反应室的底端；反应室上设有多功能进样口，用于控制实验组变量；培养基液体由总进样口投入，通过分流进样口流入不同反应单元的反应室中，通过多功能进样口对实验组变量进行控制。
The invention provides a device for inducing carbonate minerals by halophilic bacteria, which comprises a sample introduction device and several reaction units; A number of connected split injection ports; the reaction unit includes a reaction chamber, a filter device, a pH adjustment device and a temperature control stirring device, and the pH adjustment device is arranged at the top of the reaction chamber, including an acid liquid tank and an alkali liquid tank. The pH value of the reaction chamber is controlled by the amount of liquid in the reaction chamber; the temperature control stirring device includes a stirrer and a heater for adjusting and controlling the temperature, which is used to stir and heat the reaction liquid in the reaction chamber; the filter device is detachably arranged in the The bottom end of the reaction chamber; the reaction chamber is provided with a multi-function injection port to control the variables of the experimental group; the medium liquid is input from the general injection port, and flows into the reaction chambers of different reaction units through the split injection port, and passes through the multi-function injection port. The functional inlet controls the experimental group variables.
Description
Technical field
The present invention relates to carbonate rock ensaying technical fields, and in particular to one kind induces carbonate mine for Halophiles
The device of object.
Background technique
In recent years, due to the extensive use of microbial mineralization, obtaining mineral using microorganism induction becomes the research of people
Hot spot.Halophiles is the important monoid in extreme microorganism, due to special with unique physiology, biochemistry and cytology etc.
Sign, current and future all have important researching value in fields such as environment, chemical industry and biotechnologies, thus by the country
The outer more and more extensive concerns of scholar.A kind of device of Halophiles induction carbonate mineral is invented in research, is able to solve resistance to height
Salt, fluid Mass and heat transfer and mineral product efficiently separate and the key technology difficulties such as analysis, is that the carbon sequestration mineralising of Halophiles converts
The relevant environment of process, learn and biological study provides important intellectual property support.
However, in Halophiles induction mineral under carrying out different salinity, different magnesium calcium sources, different magnesium calcium ratios, conventional procedure
Extremely cumbersome, process flow is excessively complicated.
Summary of the invention
In view of the above-mentioned problems in the prior art, the present invention provides it is a kind of for Halophiles induction carbonate mineral device,
Equipped with total injection port and several reaction members, induces multiple reaction members of mineral reaction in parallel, differential responses unit is set,
Achievable total can carry out experimental group experiment simultaneously.
The following technical solution is employed by the present invention:
A kind of device for Halophiles induction carbonate mineral, including sampling device and several reaction members；It is described
Sampling device includes total injection port for launching medium liquid and several split sampling mouths being connected with total injection port；Institute
Stating reaction member includes that reaction chamber, filter for installation, pH regulating device and temperature control agitating device, the pH regulating device are set
It is placed in the top of the reaction chamber, including acid solution tank and alkali liquid tank, is controlled by adjusting the liquid inlet volume of the two anti-in reaction chamber
Answer the pH value of liquid；The temperature control agitating device includes blender and the heater for adjusting and controlling temperature, to reaction
Indoor reaction solution stirs and heats；The filter for installation is removable installed in the bottom end of reaction chamber；On reaction chamber
Equipped with Multifunctional sample injection mouth, for controlling experimental group variable.
In above scheme, medium liquid is put by total injection port, flows into differential responses unit by split sampling mouth
In reaction chamber, experimental group variable is controlled by Multifunctional sample injection mouth.
It induces multiple reaction members of mineral reaction in parallel, differential responses unit is set, it can be achieved that total can be simultaneously
Carry out experimental group experiment.
Halophiles induces carbonate mineral under different salinity, different magnesium calcium sources, different magnesium calcium ratios in reaction chamber, passes through
The mineral filter device of the separable disassembly in lower section, is separated by filtration carbonate mineral precipitating, and analysis measures its ingredient and phase character；
Temperature controls agitating device, stirs and evenly mixs fluid nutrient medium, is in 37 DEG C using heater control reaction chamber temperature.
Total injection port is mainly used for the injection of NaCl, Na2CO3, NaHCO3 solution and Halophiles bacterium solution.Multifunctional sample injection mouth
It is mainly used for controlling experimental group variable, facilitates setting different salinity, different magnesium calcium sources, different magnesium calcium ratios, induce carbonate mine
Object.
Preferably, the split sampling mouth is split sampling mouth with a scale, for accurately controlling culture medium each component
Dosage.
Preferably, the lower end of the reaction chamber is to be designed using reverse frustoconic.Conducive to the collection of mineral precipitation.
Preferably, the material of the reaction chamber uses height Salt-tolerant Materials polypropylene.Can high pressure sterilization, easy to disassemble group
Dress.
Preferably, the filter for installation is set to the bottom end of reaction chamber and is detachably connected as being threadedly coupled.Filter
Device is designed as the filter of the detachable assembling of screw thread, and carbonate mineral precipitating is extracted in special filtering, to facilitate mineral extraction point
Analysis.
The invention has the advantages that:
Different salinity, magnesium calcium source and magnesium calcium connect operation than induction mineral and carbonate mineral extraction process integration
It is continuous to carry out, time and energy is greatly saved, Halophiles is made to induce mine under different salinity, different magnesium calcium sources, different magnesium calcium ratios
It is more quick when object, it is more convenient to be separated by filtration carbonate mineral precipitating；
Each junction leakproofness is good, prevents microbiological contamination；
Package unit uses height Salt-tolerant Materials polypropylene, can high pressure sterilization, assembling easy to disassemble greatly improves work
Make efficiency；
Setting one quantitative launches switchs, and quantitatively launches different amounts of salt and magnesium calcium solution, and more convenient setting is different
The culture medium of gradient；
Separation and subsequent characterizations in time are needed to final carbonate mineral precipitating, a detachable assembling is arranged in each unit
Filter device, carbonate mineral precipitating is extracted in special filtering, and to facilitate mineral to extract, more easily measurement carbonate mineral is heavy
It forms sediment.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that the device of carbonate mineral is induced for Halophiles.
Wherein, 1 is total injection port, and 2 be Multifunctional sample injection mouth, and 3 be pH regulating device, and 4 be heater, and 5 be with a scale
Split sampling mouth, 6 be blender, and 7 be filter membrane, and 8 be filter.
Specific embodiment
The present invention is specifically described with reference to the accompanying drawing:
In conjunction with Fig. 1, a kind of device for Halophiles induction carbonate mineral, including sampling device and several reaction lists
Member；The sampling device include total injection port for launching medium liquid and be connected with total injection port several be diverted into
Sample mouth；The reaction member includes reaction chamber, filter for installation, pH regulating device and temperature control agitating device, the pH tune
Regulating device is set to the top of the reaction chamber, including acid solution tank and alkali liquid tank, is controlled by adjusting the liquid inlet volume of the two anti-
Answer the pH value of indoor reaction solution；The temperature control agitating device includes blender and the heater for adjusting and controlling temperature, is used
To be stirred and heated to the indoor reaction solution of reaction；The filter for installation is removable installed in the bottom end of reaction chamber；
The reaction chamber is equipped with Multifunctional sample injection mouth.
The split sampling mouth is split sampling mouth with a scale, for accurately controlling the dosage of culture medium each component；
The lower end of the reaction chamber is to be designed using reverse frustoconic；The material of the reaction chamber uses height Salt-tolerant Materials polypropylene；Institute
It states filter for installation and is set to the bottom end of reaction chamber and be detachably connected as being threadedly coupled, be arranged on the filter of filter for installation
There is filter membrane, filter for installation is designed as the filter of the detachable assembling of screw thread, and carbonate mineral precipitating is extracted in special filtering, with side
Just mineral extraction and analysis.
Medium liquid is put by total injection port, is flowed into the reaction chamber of differential responses unit by split sampling mouth, is led to
Multi-functional injection port is crossed to control experimental group variable.
Embodiment 1
Control different magnesium sources:
After the device is carried out high pressure sterilization, blender 6, the same calcium source (CaCl of fluid nutrient medium are opened2·2H2O), salt
It spends (15%), fluid nutrient medium (is contained into CaCl2·2H2O it) is put into, passes through according to 0,3,6,9,12 ratio more from total injection port 1
Function injection port 2 is separately added into required MgCl2·6H2O, after mixing evenly high pressure sterilization.By total injection port, to each anti-
Unit is answered to be injected separately into 1mol/LNa2CO3、NaHCO3Solution surveys its pH with pH regulating device 3 after stirring evenly, passes through adjusting
The liquid inlet volume of acid solution tank and alkali liquid tank controls the pH value of reaction chamber, makes pH stable 7 or so.According to chamber volume
10% adds Halophiles bacterium solution from total injection port 1, and setting temperature controls 37 DEG C of agitating device temperature, successive reaction 14 days.It opens
Filter device switch, fluid nutrient medium outflow, carbonate mineral precipitating are obtained by filtration by filter membrane 7, and rotation screw thread is detachable
Filter 8 collects carbonate mineral precipitating, carries out mineral product phenetic analysis.
When the different magnesium source of experiment control, fluid nutrient medium is consistent with the above, same calcium source (CaCl2·2H2O), salinity
(15%), fluid nutrient medium (is contained into CaCl2·2H2O it) is put into from total injection port 1, passes through more function according to 0,3,6,9,12 ratio
Energy injection port 2 is separately added into required MgSO4·7H2O also embodies the Multifunctional sample injection mouth and is mainly used for control experiment
Group variable.Carry out operation same as described above.High pressure sterilization after mixing evenly.Total 1 filter of injection port is opened, is injected separately into
1mol/LNa2CO3、NaHCO3Solution surveys its pH with pH regulating device 3 after stirring evenly, by adjusting acid solution tank and alkali liquid tank
Liquid inlet volume control the pH value of reaction chamber, make pH stable 7 or so.According to chamber volume 10% from total injection port 1
Halophiles bacterium solution is added, setting temperature controls 37 DEG C of agitating device temperature, successive reaction 14 days.Open filter device switch, liquid
The outflow of body culture medium, carbonate mineral precipitate logical filter membrane 7 and are obtained by filtration, and rotate screw thread detachable filter 8, collect carbonate
Mineral precipitation carries out mineral product phenetic analysis.
Embodiment 2
Control different calcium sources:
After the device is carried out high pressure sterilization, blender 6, the same magnesium source (MgSO of fluid nutrient medium are opened4·7H2O), salt
It spends (15%), fluid nutrient medium (is contained into MgSO4·7H2O it) is put into, passes through according to 0,3,6,9,12 ratio more from total injection port 1
Function injection port 2 is separately added into required C4CaH6O4·H2O carries out operation same as described above.High pressure sterilization after mixing evenly.
By total injection port, 1mol/LNa is injected separately into each reaction member2CO3、NaHCO3Solution, with pH tune after stirring evenly
Regulating device 3 surveys its pH, controls the pH value of reaction chamber by adjusting the liquid inlet volume of acid solution tank and alkali liquid tank, makes pH stable 7
Left and right.Halophiles bacterium solution is added from total injection port 1 according to the 10% of chamber volume, setting temperature controls agitating device temperature 37
DEG C, successive reaction 14 days.Filter device switch, fluid nutrient medium outflow are opened, carbonate mineral precipitating is filtered by filter membrane 7
It obtains, rotates screw thread detachable filter 8, collect carbonate mineral precipitating, carry out mineral product phenetic analysis.
Embodiment 3
Control different salinity:
After the device is carried out high pressure sterilization, blender 6, the same calcium source (CaCl of fluid nutrient medium are opened2·2H2O), same
One magnesium source (MgSO4·7H2O), fluid nutrient medium (is contained into CaCl2·2H2O、MgSO4·7H2O it) is put into from total injection port 1, according to
0,3,6,9,12 ratio is separately added into required MgSO by Multifunctional sample injection mouth 24·7H2O.High pressure sterilization after mixing evenly.
By total injection port, 1mol/LNa is injected separately into each reaction member2CO3、NaHCO3Solution, with pH tune after stirring evenly
Regulating device 3 surveys its pH, controls the pH value of reaction chamber by adjusting the liquid inlet volume of acid solution tank and alkali liquid tank, makes pH stable 7
Left and right.Halophiles bacterium solution is added from total injection port 1 according to the 10% of chamber volume, setting temperature controls agitating device temperature 37
DEG C, successive reaction 14 days.Filter device switch, fluid nutrient medium outflow are opened, carbonate mineral precipitates logical filter membrane 7 and filtered
It arrives, rotates screw thread detachable filter 8, collect carbonate mineral precipitating, carry out mineral product phenetic analysis.
Required NaCl is added in liquid medium according to 5%, 10%, 15%, 20%, 25% salinity.It carries out identical
Operation repeats above-mentioned experiment five time.
Certainly, the above description is not a limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The variations, modifications, additions or substitutions that the technical staff in domain is made within the essential scope of the present invention also should belong to of the invention
Protection scope.
Claims (5)
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070479A (en) * | 2013-12-16 | 2015-06-25 | 연세대학교 산학협력단 | Reactor for inorganic carbonation |
| CN106018751A (en) * | 2016-07-01 | 2016-10-12 | 山东科技大学 | Carbonate mineral dolomitization simulation study system and application method thereof |
| US20170182458A1 (en) * | 2015-12-29 | 2017-06-29 | Yuanchu Technology (Beijing) Co., Ltd. | Apparatus and method for absorbing and mineralizing carbon dioxide |
| CN108395983A (en) * | 2018-01-12 | 2018-08-14 | 山东科技大学 | A kind of configuration of culture medium and mineral obtain system |
| CN208279358U (en) * | 2018-01-25 | 2018-12-25 | 嘉兴市欣欣仪器设备有限公司 | Laboratory experiment use in waste water treatment coagulation tank |
| CN209685782U (en) * | 2019-02-19 | 2019-11-26 | 山东科技大学 | A device for inducing carbonate minerals by halophilic bacteria |
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- 2019-02-19 CN CN201910123633.4A patent/CN109593644A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150070479A (en) * | 2013-12-16 | 2015-06-25 | 연세대학교 산학협력단 | Reactor for inorganic carbonation |
| US20170182458A1 (en) * | 2015-12-29 | 2017-06-29 | Yuanchu Technology (Beijing) Co., Ltd. | Apparatus and method for absorbing and mineralizing carbon dioxide |
| CN106018751A (en) * | 2016-07-01 | 2016-10-12 | 山东科技大学 | Carbonate mineral dolomitization simulation study system and application method thereof |
| CN108395983A (en) * | 2018-01-12 | 2018-08-14 | 山东科技大学 | A kind of configuration of culture medium and mineral obtain system |
| CN208279358U (en) * | 2018-01-25 | 2018-12-25 | 嘉兴市欣欣仪器设备有限公司 | Laboratory experiment use in waste water treatment coagulation tank |
| CN209685782U (en) * | 2019-02-19 | 2019-11-26 | 山东科技大学 | A device for inducing carbonate minerals by halophilic bacteria |
Non-Patent Citations (1)
| Title |
|---|
| 李福春;郭文文;: "三种好氧细菌诱导碳酸钙矿物的形成", 南京大学学报(自然科学版), no. 06, pages 665 - 672 * |
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