CN107667930A - It is easy to gather the breeding spaces and acquisition method of Daphnia magna - Google Patents

It is easy to gather the breeding spaces and acquisition method of Daphnia magna Download PDF

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Publication number
CN107667930A
CN107667930A CN201711068355.4A CN201711068355A CN107667930A CN 107667930 A CN107667930 A CN 107667930A CN 201711068355 A CN201711068355 A CN 201711068355A CN 107667930 A CN107667930 A CN 107667930A
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CN
China
Prior art keywords
daphnia magna
enrichment
breeding spaces
gather
easy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711068355.4A
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Chinese (zh)
Inventor
于伟丽
吴培
高德良
苏磊
陈乐乐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANDONG ACADEMY OF PESTICIDE SCIENCES
Original Assignee
SHANDONG ACADEMY OF PESTICIDE SCIENCES
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Filing date
Publication date
Application filed by SHANDONG ACADEMY OF PESTICIDE SCIENCES filed Critical SHANDONG ACADEMY OF PESTICIDE SCIENCES
Priority to CN201711068355.4A priority Critical patent/CN107667930A/en
Publication of CN107667930A publication Critical patent/CN107667930A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/20Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/60Floating cultivation devices, e.g. rafts or floating fish-farms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of breeding spaces and acquisition method for being easy to gather Daphnia magna, breeding spaces include:The culture zone separated by flashboard and enrichment region, enrichment region is provided with enrichment plate and light source, fibrous layer is provided with enrichment plate, culture zone is provided with the light shield that can be folded, Daphnia magna can freely pass through the mesh of the grid, flashboard, which includes the demarcation strip being close together and grid, demarcation strip, can detach breeding spaces.Acquisition method lights the light source in enrichment region including S1, and the light shield expansion of culture zone is sheltered from into the periphery of culture zone, the demarcation strip on flashboard is detached out, Daphnia magna is freely passed through grid;S2 inserts food bait on the fibrous layer into enrichment region;Enrichment plate is detached enrichment region and is transferred in collecting pit by S3.

Description

It is easy to gather the breeding spaces and acquisition method of Daphnia magna
Technical field
The present invention relates to a kind of Daphnia magna to cultivate harvesting technique, more particularly to a kind of breeding spaces for being easy to gather Daphnia magna And acquisition method, Daphnia magna are widely used in field of Environment Protection.
Background technology
Daphnia magna carries out the research of Pesticide environment safety evaluatio extensively, widely should have in agricultural chemicals toxicological test With.Laboratory is bigger for the demand of Daphnia magna.Particularly the primary Daphnia magna of nature is, it is necessary to measure bigger, it is necessary to special Fixed cultivation and collecting method, to meet the larger demand of experiment.
The content of the invention
Instant invention overcomes the deficiencies in the prior art, there is provided a kind of breeding spaces and collection side for being easy to gather Daphnia magna Method.
To reach above-mentioned purpose, a kind of technical scheme that the present invention uses for:It is a kind of to be easy to the cultivation for gathering Daphnia magna empty Between, including:The culture zone separated by flashboard and enrichment region, the enrichment region are provided with enrichment plate and light source, the enrichment plate On be provided with fibrous layer, the culture zone is provided with the light shield that can be folded, and the flashboard includes the separation being close together Plate and grid, Daphnia magna can freely pass through the mesh of the grid, and the demarcation strip can detach breeding spaces.
In a preferred embodiment of the present invention, the light source is area source, and an institute is respectively provided with each enrichment plate State area source.
In a preferred embodiment of the present invention, the enrichment plate plane is perpendicular to the grid plane, some pieces of richnesses Collection plate is parallel to each other and alternate is arranged in the enrichment region.
In a preferred embodiment of the present invention, the breeding spaces are net cage, and the net cage is placed in water body, Daphnia magna The mesh of the net cage can not be passed through.
In a preferred embodiment of the present invention, the water body is river, lake or pond.
In a preferred embodiment of the present invention, under cultivating condition, demarcation strip on flashboard by culture zone and acquisition zone every From Daphnia magna can not traverse to enrichment region from culture zone.
In a preferred embodiment of the present invention, the wave-length coverage of the light source is 460nm-470nm.
The another technical solution that the present invention uses for:A kind of method that Daphnia magna collection is carried out in breeding spaces, bag Include following steps:
S1 lights the light source in enrichment region, the light shield expansion of culture zone is sheltered from into the periphery of culture zone, by flashboard On demarcation strip detach out, Daphnia magna is freely passed through grid;
S2 inserts food bait on the fibrous layer into enrichment region;
Enrichment plate is detached enrichment region and is transferred in collecting pit by S3.
In a preferred embodiment of the present invention, the step S2 duration is -150 minutes 15 minutes.
In a preferred embodiment of the present invention, every 15 to 30 minutes, a food bait is inserted.
The present invention solves defect present in background technology, and the present invention possesses following beneficial effect:
(1) Daphnia magna is cultivated in nature environment, Daphnia magna is cultivated in culturing area by the form of net cage, Net cage is mutually isolated by the Daphnia magna inside and outside it, prevents that intersecting breeding influences the pure of Daphnia magna species.Net cage can not simultaneously The disconnected water body with nature carries out inside and outside exchange, ensures that the growing environment of Daphnia magna is substantially consistent with the Daphnia magna of nature, its Initial resistance to malicious ability is coincide substantially with nature.
(2) enrichment region can lure Daphnia magna to realize that the enrichment of Daphnia magna harvests by light and food bait, while Daphnia magna and the separation of cultivation raising thing more mixed and disorderly in culture zone are realized, the Daphnia magna of collection is cleaner, eliminates The step of great effort removes the impurity dirt of incoming call is expended after collection.
(3) carrier that the fibrous layer set on plate provides enrichment absorption for Daphnia magna is enriched with, and plate will be enriched with from water In can prevent when detaching Daphnia magna from departing from enrichment plate.Area source can attract Daphnia magna to be moved about to enrichment region.
(4) light shield corresponding light source is set, and it provides lucifuge condition for culturing area, and the light source for setting off by contrast enrichment region is made With guarantee enrichment region and culture zone light condition contrast are bigger, embody the effect that light lures Daphnia magna as far as possible.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples;
Fig. 1 is the schematic top plan view under cultivating condition of the preferred embodiments of the present invention;
Fig. 2 is the schematic top plan view under acquisition condition of the preferred embodiments of the present invention;
In figure:1st, culture zone;2nd, enrichment region;3rd, light shield;4th, demarcation strip;5th, grid;6th, area source;7th, fibrous layer.
Embodiment
Presently in connection with drawings and examples, the present invention is further detailed explanation, and these accompanying drawings are simplified signal Figure, only illustrate the basic structure of the present invention in a schematic way, therefore it only shows the composition relevant with the present invention.
As depicted in figs. 1 and 2, a kind of breeding spaces for being easy to gather Daphnia magna, including:The culture zone separated by flashboard And enrichment region, it is characterised in that enrichment region is provided with enrichment plate and light source, is enriched with plate and is provided with fibrous layer, and culture zone is set There is the light shield that can be folded, flashboard, which includes the demarcation strip being close together and grid, Daphnia magna, can freely pass through the net of grid Hole, demarcation strip can detach breeding spaces.
Daphnia magna is cultivated in nature environment, Daphnia magna, net are cultivated in culturing area by the form of net cage Case is mutually isolated by the Daphnia magna inside and outside it, prevents that intersecting breeding influences the pure of Daphnia magna species.Net cage can be continuous simultaneously With nature water body carry out it is inside and outside exchange, ensure that the growing environment of Daphnia magna is substantially consistent with the Daphnia magna of nature, at the beginning of it The resistance to malicious ability that begins is coincide substantially with nature.
Enrichment region can lure Daphnia magna to realize the enrichment harvesting of Daphnia magna by light and food bait, while also realize Daphnia magna and the separation of cultivation raising thing more mixed and disorderly in culture zone, the Daphnia magna of collection is cleaner, eliminate collection The step of great effort removes the impurity dirt of incoming call is expended afterwards.
The fibrous layer set on enrichment plate provides the carrier of enrichment absorption for Daphnia magna, and is taken out by enrichment plate from water From when can prevent Daphnia magna depart from enrichment plate.Area source can attract Daphnia magna to be moved about to enrichment region.
Light source is area source, and each be enriched with plate is respectively provided with an area source, is enriched with plate plane perpendicular to grid plane, if Dry block enrichment plate is parallel to each other and alternate is arranged in enrichment region.Light shield corresponding light source is set, and it provides for culturing area and kept away Optical condition, set off by contrast the light source effect of enrichment region, ensure that enrichment region and culture zone light condition contrast are bigger, embody as far as possible Light lures the effect of Daphnia magna.
Breeding spaces are net cage, and net cage is placed in water body, and Daphnia magna can not pass through the mesh of net cage, water body be river, Lake or pond.
Under cultivating condition, the demarcation strip on flashboard isolates culture zone and acquisition zone, and Daphnia magna can not be worn from culture zone Enrichment region is more arrived, the wave-length coverage of light source is 460nm-470nm.
A kind of method that Daphnia magna collection is carried out in breeding spaces, comprises the following steps:
S1 lights the light source in enrichment region, the light shield expansion of culture zone is sheltered from into the periphery of culture zone, by flashboard On demarcation strip detach out, Daphnia magna is freely passed through grid;
S2 inserts food bait on the fibrous layer into enrichment region;
Enrichment plate is detached enrichment region and is transferred in collecting pit by S3.
The step S2 duration is -150 minutes 15 minutes, every 15 to 30 minutes, inserts a food bait.
The desirable embodiment according to the present invention is enlightenment above, and by above-mentioned description, related personnel completely can be with Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention The content being not limited on specification, it is necessary to determine the technical scope according to the scope of the claims.

Claims (10)

1. a kind of breeding spaces for being easy to gather Daphnia magna, including:The culture zone separated by flashboard and enrichment region, its feature exist In the enrichment region is provided with enrichment plate and light source, is provided with fibrous layer on the enrichment plate, the culture zone is provided with can The light shield of folding, the flashboard, which includes the demarcation strip being close together and grid, Daphnia magna, can freely pass through the grid Mesh, the demarcation strip can detach breeding spaces.
A kind of 2. breeding spaces for being easy to gather Daphnia magna according to claim 1, it is characterised in that:The light source is face Light source, each described be enriched with plate are respectively provided with an area source.
A kind of 3. breeding spaces for being easy to gather Daphnia magna according to claim 1 or 2, it is characterised in that:The enrichment Perpendicular to the grid plane, some blocks of enrichment plates are parallel to each other and alternate are arranged in the enrichment region plate plane.
A kind of 4. breeding spaces for being easy to gather Daphnia magna according to claim 1, it is characterised in that:The breeding spaces For net cage, the net cage is placed in water body, and Daphnia magna can not pass through the mesh of the net cage.
A kind of 5. breeding spaces for being easy to gather Daphnia magna according to claim 4, it is characterised in that:The water body is river Stream, lake or pond.
A kind of 6. breeding spaces for being easy to gather Daphnia magna according to claim 1, it is characterised in that:In cultivating condition Under, the demarcation strip on flashboard isolates culture zone and acquisition zone, and Daphnia magna can not traverse to enrichment region from culture zone.
A kind of 7. breeding spaces for being easy to gather Daphnia magna according to claim 1, it is characterised in that:The ripple of the light source Long scope is 460nm-470nm.
8. the method for Daphnia magna collection is carried out in a kind of breeding spaces in claim 1-7 described in any claim, its It is characterised by, comprises the following steps:
S1 lights the light source in enrichment region, the light shield expansion of culture zone is sheltered from into the periphery of culture zone, by flashboard Demarcation strip detaches out, and Daphnia magna is freely passed through grid;
S2 inserts food bait on the fibrous layer into enrichment region;
Enrichment plate is detached enrichment region and is transferred in collecting pit by S3.
9. acquisition method according to claim 8, it is characterised in that:The step S2 duration is -150 minutes 15 minutes.
10. acquisition method according to claim 9, it is characterised in that:Every 15 to 30 minutes, insert a food and lure Bait.
CN201711068355.4A 2017-11-03 2017-11-03 It is easy to gather the breeding spaces and acquisition method of Daphnia magna Pending CN107667930A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111233158A (en) * 2020-02-27 2020-06-05 中海生态环境科技有限公司 Algae and insect removing cultivation optimization method and cultivation optimization device thereof
CN111387100A (en) * 2019-09-12 2020-07-10 浙江海洋大学 Separation and purification and large-scale culture method for daphnia fusiforme
CN112493185A (en) * 2020-11-30 2021-03-16 广东大麟洋海洋生物有限公司 Ecological breeding method for golden pomfret

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101112192A (en) * 2007-08-31 2008-01-30 浙江省海洋水产养殖研究所 Device for catching plankton
CA2642397A1 (en) * 2008-11-20 2010-05-20 Gerard Voon Bioscience viii
CN105016472A (en) * 2015-05-28 2015-11-04 史建新 Applicaton method of scrap tires in process of steadily converting alga type lake into grass type lake
CN107094721A (en) * 2017-07-10 2017-08-29 佛山市水创科联生态科技有限公司 The primary Daphnia magna enriching apparatus in field and enrichment method
CN107125186A (en) * 2017-07-10 2017-09-05 佛山市水创科联生态科技有限公司 Daphnia magna cultivation collection pond and acquisition method
CN207707063U (en) * 2017-11-03 2018-08-10 山东省农药科学研究院 Convenient for acquiring the breeding spaces of Daphnia magna

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101112192A (en) * 2007-08-31 2008-01-30 浙江省海洋水产养殖研究所 Device for catching plankton
CA2642397A1 (en) * 2008-11-20 2010-05-20 Gerard Voon Bioscience viii
CN105016472A (en) * 2015-05-28 2015-11-04 史建新 Applicaton method of scrap tires in process of steadily converting alga type lake into grass type lake
CN107094721A (en) * 2017-07-10 2017-08-29 佛山市水创科联生态科技有限公司 The primary Daphnia magna enriching apparatus in field and enrichment method
CN107125186A (en) * 2017-07-10 2017-09-05 佛山市水创科联生态科技有限公司 Daphnia magna cultivation collection pond and acquisition method
CN207707063U (en) * 2017-11-03 2018-08-10 山东省农药科学研究院 Convenient for acquiring the breeding spaces of Daphnia magna

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111387100A (en) * 2019-09-12 2020-07-10 浙江海洋大学 Separation and purification and large-scale culture method for daphnia fusiforme
CN111387100B (en) * 2019-09-12 2021-10-22 浙江海洋大学 Separation and purification and large-scale culture method for daphnia fusiforme
CN111233158A (en) * 2020-02-27 2020-06-05 中海生态环境科技有限公司 Algae and insect removing cultivation optimization method and cultivation optimization device thereof
CN112493185A (en) * 2020-11-30 2021-03-16 广东大麟洋海洋生物有限公司 Ecological breeding method for golden pomfret

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Application publication date: 20180209