CN104365557B - A kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method - Google Patents
A kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method Download PDFInfo
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- 241001294750 Pediobius foveolatus Species 0.000 claims description 6
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- 241000219112 Cucumis Species 0.000 claims description 5
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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Abstract
The invention discloses a kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method.The present invention selects the host plant kind of efficient host egg mealybug；Ladybug was raised by its ovum, larva, pupa and four stages of development of adult by stages, and be found that this lay eggs lure collection thing extractive technique, and in conjunction with this ladybug lay eggs mechanism collect this Ladybird egg；Propose the suitable breeding technology of ladybug different phase, temperature and humidity conditions and condition of storage；Adult is box-packaged, larva, ovum paper bag type packaging, it is simple to field adaptability and popularization and application.Achieve high-quality, efficiently, high-volume breed Cryptolaemus montrouzieri, meet the needs of production.
Description
Technical field:
The invention belongs to natural enemy insect artificial breeding field, be specifically related to a kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method.
Background technology:
Species of ladybirds is various, mostly as the outstanding predator of agriculture and forestry injurious insect, protects and utilize the superiority of natural enemy insect, controls agriculture and forestry injurious insect and has caused ecological and Agricultural Scientist concern, at home and abroad more existing success examples.In recent years, the research about the man-made feeds of ladybug and about aspects such as ecological, biological characteristics makes significant progress.But its scale and standardization breeding technology need perfect; bigger problem is all there is in breeding facility, production in enormous quantities technological standards, packing and storing technology etc.; therefore limit that outstanding natural enemy insect is in good time, substantial amounts of supply, it is impossible to meet the needs produced at present.
Summary of the invention:
It is an object of the invention to overcome above-mentioned deficiency of the prior art, it is provided that a kind of high-quality, efficient, production in enormous quantities Cryptolaemus montrouzieri, meet the relevant Cryptolaemus montrouzieri artificial breeding of needs of Pest control of agricultural, conservation and propagation production method.
The Cryptolaemus montrouzieri artificial breeding of the present invention, conservation and propagation production method, it is characterised in that comprise the following steps:
(1), mealybug cultivate: on the south melon and fruit implementation be host plant cultivate mealybug；
(2), ladybug is cultivated:
A. ovum period management: place defat cotton-wool in box raising, and smear to lay eggs and lure collection thing mealybug waxiness, the Cryptolaemus montrouzieri of emergence copulation is laid eggs on cotton-wool, collects cotton-wool standby or be placed in ventilative plastic casing, and cold preservation is standby when 14-17 DEG C；
B. larva is raised in cages: at temperature 24-28 DEG C, relative humidity 40-75%, under the condition of illumination 14 hour/day, is put on the pallet in dependent insect cage by the Fructus Cucurbitae moschatae with mealybug in step (1)；Receiving on Fructus Cucurbitae moschatae by the cotton-wool with Cryptolaemus montrouzieri ovum collected, after larvae hatch, on Fructus Cucurbitae moschatae, the quantity of mealybug is gradually increased, and ladybug of leaving takes food growth, until the mealybug on Fructus Cucurbitae moschatae takes food totally, only during surplus mealybug wax silk, puts into the new Fructus Cucurbitae moschatae being covered with mealybug；
C, pupa time: temperature 24-28 DEG C, when relative humidity 40-75%, when Pediobius foveolatus length to 4 mature larva in latter stage in age, it is chosen in dependent insect cage and is placed in culture dish, and put into mealybug and ensure that its foodstuff is abundant, until pupating；
D. the emergence of adult: ladybug pupa is put into 24-28 DEG C, grows when relative humidity 40-75%, raises after emergence, and during raising, female-male proportion is 1:1, supplements mealybug simultaneously and supplements the nutrients, to guarantee that adult can normal growth and breeding so that it is lay eggs.
On the south described, melon and fruit implementation is that host plant cultivates mealybug concrete steps preferably: 24-28 DEG C, relative humidity 50-75%, under the condition of illumination 14 hour/day, choose the Fructus Cucurbitae moschatae that surface is comparatively coarse, clean with wet cloth, after drying, it is placed on shelf bracket, put up a bridge with the paper slip of width 1.5 ± 0.5cm, connect the Fructus Cucurbitae moschatae newly bought and cover with the Fructus Cucurbitae moschatae of mealybug, mealybug crawl on new host so that can shift, after 20-30 days, mealybug covers with when covering fresh Fructus Cucurbitae moschatae, just can be used for raising Pediobius foveolatus and adult, if period put up a bridge paper slip mouldy or be covered with mealybug secretion honeydew time, to change paper slip in time.
Described mealybug waxiness is to leach the mealybug with ootheca with volume fraction 99% aqueous acetone solution, removes the filtrate filter paper filtering after polypide and silk quality material, and filtering residue is mealybug waxiness after drying.
In whole artificial propagation process, it is possible to obtain the ovum of Cryptolaemus montrouzieri, larva and adult, adult can adopt box-packaged, larva, ovum paper bag type packaging.Ladybird egg grain cold preservation 14-17 DEG C, relative humidity 55-75% when, it is possible to extend the incubation period.Grandis adults emergence carried out cold preservation after 7-10 days in temperature 10 DEG C, relative humidity 55-75% when, and every 6 days of period fed a hydromel and supplements the nutrients, and storage life reaches about 60 days.
The present invention has the following advantages: host plant kind and the mealybug of selecting efficient host egg mealybug obtain technology in a large number；Cryptolaemus montrouzieri was raised by its ovum, larva, pupa and four stages of development of adult by stages, adopted suitable breeding technology according to its biology, ecological characteristics；Development test amount reproduction utensil, improves reproductive efficiency；Physics and chemical method is utilized to lure collection ladybug to lay eggs, it is simple to centralized collection is cultivated；Test determines raising and condition of storage；Adult is box-packaged, larva, ovum paper bag type packaging, it is simple to field adaptability and popularization and application.Explore the key technology utilizing live body mealybug high-volume to breed ladybug, it is achieved that high-quality, efficiently, high-volume breed ladybug, be used for preventing and treating the agriculture and forestry injurious insects such as mealybug, meet the needs of production.
The present invention selects the host plant kind of efficient host egg mealybug；Ladybug was raised by its ovum, larva, pupa and four stages of development of adult by stages, and be found that this lay eggs lure collection thing extractive technique, and in conjunction with this ladybug lay eggs mechanism collect this Ladybird egg；Propose the suitable breeding technology of ladybug different phase, temperature and humidity conditions and condition of storage；Adult is box-packaged, larva, ovum paper bag type packaging, it is simple to field adaptability and popularization and application.Achieve high-quality, efficiently, high-volume breed Cryptolaemus montrouzieri, meet the needs of production.
Accompanying drawing illustrates:
Fig. 1 is the flow chart of the Cryptolaemus montrouzieri artificial breeding of the present invention, conservation and propagation production method.
Detailed description of the invention:
Following example are further illustrating the present invention, rather than limitation of the present invention.
Embodiment 1:
Referring to Fig. 1, the step of the artificial breeding of the present embodiment, conservation and propagation production Cryptolaemus montrouzieri is as follows:
(1), mealybug cultivate: on the south melon and fruit implementation be host plant cultivate mealybug；24-28 DEG C, relative humidity 50-75%, under the condition of illumination 14 hour/day, choose the Fructus Cucurbitae moschatae that surface is comparatively coarse, clean with wet cloth, after drying, it is placed on shelf bracket, puts up a bridge with the paper slip of width 1.5 ± 0.5cm, connect the Fructus Cucurbitae moschatae newly bought and cover with the Fructus Cucurbitae moschatae of mealybug, mealybug crawl on new host so that can shift, after 20-30 days, mealybug covers with when covering fresh Fructus Cucurbitae moschatae, just can be used for raising Pediobius foveolatus and adult, if period put up a bridge paper slip mouldy or be covered with mealybug secretion honeydew time, paper slip will be changed in time；
(2), ladybug is cultivated:
A. ovum period management: Cryptolaemus montrouzieri pupa is put into 24-28 DEG C, grows when relative humidity 40-75%, raises after emergence, and during raising, female-male proportion is 1:1, and ladybug carries out cold preservation at temperature 10 DEG C and lays eggs or be directly used in and lay eggs after raising one week；Ladybug starts to lay eggs after sprouting wings for 5-7 days, defat cotton-wool is placed in box raising, and smear lay eggs lure collection thing (mealybug waxiness), the Cryptolaemus montrouzieri that copulation is sprouted wings is laid eggs in the gap of cotton-wool, collect cotton-wool, the cotton-wool collected every day is standby or is placed in ventilative plastic casing, and cold preservation standby when 14-17 DEG C (can extend hatching in 10 days, be normally about 5 days)；
B. larva is raised in cages: at temperature 24-28 DEG C, relative humidity 40-75%, under the condition of illumination 14 hour/day, the mealybug in step (1) is climbed full Fructus Cucurbitae moschatae surface 90% Fructus Cucurbitae moschatae put on the pallet in aluminium alloy dependent insect cage (inoculation mealybug about 30 days)；The cotton-wool with Cryptolaemus montrouzieri ovum collected uniformly is received on Fructus Cucurbitae moschatae, probably every 4 square centimeters connect a cotton-wool, after larvae hatch, on Fructus Cucurbitae moschatae, the quantity of mealybug is also gradually increased, ladybug of leaving takes food growth, until the mealybug on Fructus Cucurbitae moschatae takes food totally, only during surplus mealybug wax silk, put into the new Fructus Cucurbitae moschatae being covered with mealybug；
C, pupa time: temperature 24-28 DEG C, when relative humidity 40-75%, when Pediobius foveolatus length to 4 mature larva (general transfixion is rolled up together) in latter stage in age, it is chosen in dependent insect cage and is placed in big plastic culture dish (9 × 1.5cm), every about 25, ware, and put into a small amount of mealybug and ensure that its foodstuff is abundant, until pupating；
D. the emergence of adult: ladybug pupa is put into 24-28 DEG C, grows when relative humidity 40-75%, raises after emergence, and during raising, female-male proportion is 1:1, supplements mealybug simultaneously and supplements the nutrients, to guarantee that adult can normal growth and breeding so that it is lay eggs.
In whole artificial propagation process, it is possible to obtain the ovum of ladybug, larva and adult, adult can adopt box-packaged, larva, ovum paper bag type packaging.Ladybird egg grain cold preservation 14-17 DEG C, relative humidity 55-75% when, it is possible to extend the incubation period.Grandis adults emergence carried out cold preservation after 7-10 days in temperature 10 DEG C, relative humidity 55-75% when, and every 6 days of period fed a hydromel and supplements the nutrients, and storage life reaches about 60 days.
Thus achieve high-quality, purpose efficient, breeding ladybug in high volume.
Test executes 1: the extraction of mealybug waxiness and activity identification
The picking of mealybug waxiness: the mealybug with ootheca is put in tool plug glass jar, being subsequently adding concentration is volume fraction 99% aqueous acetone solution, after cover lid, with hand rolling wide mouthed bottle about 10 minutes, it is then placed on desktop static, can be appreciated that and bottle has white flock precipitate precipitate out, with tweezers, the silk quality material being intertwined in bottle is chosen, remaining turbid solution rocks the mealybug polypide falling in turbid solution with the ferrum sieving of 30 sieve meshes afterwards, obtain turbid solution, then turbid solution Glass rod is drained into in the long neck funnel of filter paper, tool plug conical flask is connect below funnel.After having filtered, filter paper has white flock precipitate, in room temperature ventilation situation, flocculent deposit on filter paper will dry hardens, this whiteness hardened is exactly the waxy substance being eluted out from mealybug, i.e. mealybug waxiness, it is possible to prove that it possesses the activity luring that Cryptolaemus montrouzieri is laid eggs into by tests below.
Mealybug waxiness activity identification is tested: provide 4 kinds of oviposition substrate for Cryptolaemus montrouzieri.First, absorbent cotton；Second, impregnated the absorbent cotton of above-mentioned mealybug acetone eluant, be called for short dipping cotton: be impregnated in by absorbent cotton in the acetone eluant of mealybug, be then put under room temperature natural air drying 48h and be placed in the plastic bottle of sealing standby；3rd, smear the rayon balls of above-mentioned mealybug waxiness in side, be called for short wax cotton；4th, mealybug ootheca.These 4 kinds of substrate being respectively placed in two vertical straight lines in excessive plastic culture dish (9 × 1.5cm) center of circle intersect with inwall on 4 points of part, substrate base is all fixed with double faced adhesive tape.Choose after the similar mealybug nymph of Cryptolaemus montrouzieri female worm 8-12 head body size that copulation starting lays eggs feeds 12 hours, choose and be placed in the above-mentioned big plastic culture dish being loaded with 4 kinds of substrate, any food is not provided, number and the load ovum amount thereof of every kind of load ovum substrate is recorded after 12 hours, the number that often in group repetition, sample is laid eggs, totally 8 groups of repetitions, often group repeats 20 samples (female worm), totally 160 samples.
Calculate in often group repetition, total load ovum amount of every kind of substrate；Substrate load ovum amount accounts for total egg laying amount × 100% in the total load ovum amount/group in ratio (every kind of substrate load ovum amount proportion) the interior every kind of substrate of=group of total egg laying amount in organizing；Relative spawning rate=group contains number/female worm number × 100% of laying eggs of ovum substrate；In group, substrate is on average carried ovum amount=substrate and is always carried the number of ovum amount/load ovum substrate.Use statistical analysis software SPSS17.0 to calculate meansigma methods and standard error for above 4 observation contents, and it is carried out multiple sample correlations inspection (kRelatedSamples).
Table 1: under alternative condition, Cryptolaemus montrouzieri laying eggs on different substrates is compared
Note: in table, data are meansigma methods ± standard error, same column internal labeling same letter represent its under 0.05 level without significant difference.
As it can be seen from table 1 cotton at wax and on mealybug ootheca the egg laying amount of Cryptolaemus montrouzieri there is no significant difference, and to be significantly higher than the egg laying amount on Cotton Gossypii and dipping cotton.Illustrate that the waxiness on wax cotton has the activity luring that Cryptolaemus montrouzieri is laid eggs into.
Test executes 2: the impact (Chen pioneer etc., 2000) that Cryptolaemus montrouzieri is grown by different temperatures.
Arranging 14 DEG C in growth cabinet, 17 DEG C, 20 DEG C, 23 DEG C, 26 DEG C, 29 DEG C, 32 DEG C, 8 thermogrades such as 34 DEG C of grades, relative humidity is about 75%, illumination L: D=12: 12.The filter paper having fresh Cryptolaemus montrouzieri ovum is put in culture dish (internal diameter 120mm, high 15mm), and each thermograde places 150 ovum, observes the hatching situation of ovum.The larva 50 that larval phase hatches with same temperature, raises with citrus mealy bug in bottle (internal diameter 30mm, high 50mm), and every larva accounts for 1 bottle, and bottleneck gauze is wrapped up, in case larva escapes.Changing feedstuff, observation was casted off a skin, pupates, was sprouted wings and death condition every day in good time.Adult stage, with the 10 pairs of adults (male containing 1 female 1) just sprouted wings, is respectively placed in 10 bottles and raises, and bottleneck gauze is wrapped up.Every morning changes feedstuff, the ovum amount in the feedstuff changed out with anatomical lens inspection.Observe and start until adult natural death after Cryptolaemus montrouzieri is sprouted wings.
Table 2: under different temperatures, Cryptolaemus montrouzieri each stage of development goes through the phase (d)
Table 3: each worm state of Cryptolaemus montrouzieri and survival rate (%) from generation to generation under different temperatures
From result of the test (table 2 and table 3), within the scope of 23～29 DEG C, the development duration of Cryptolaemus montrouzieri each worm state is shorter, and there is higher survival rate, wherein with the survival rate highest order 49.7% of the generation at 26 DEG C, so the temperature of about 26 DEG C is suitable for amount reproduction Cryptolaemus montrouzieri.
Test example 3: Cotton Gossypii substitutes the cotton ovum collecting ladybug as substrate of wax
The female male imago of Cryptolaemus montrouzieri of pairing copulation is placed in little plastic culture dish (6cm × 1.5cm) and raises, and put in culture dish feeding Cryptolaemus montrouzieri 12 hours (this within 12 hours, become take food the period) from the less citrus mealy bug nymph of the body surface waxiness that picking 10-15 head build pumpkin fruit is similar, afterwards female male imago is placed in equipped with in the little plastic culture dish of corresponding oviposition substrate, female worm is allowed to lay eggs 12 hours (this is called the period of laying eggs in 12 hours), afterwards, female for Cryptolaemus montrouzieri male imago is chosen, it is placed in little plastic culture dish and continues to provide aforementioned fresh mealybug to take food by it, so repeatedly, continuously perform 10 days.Test arranges 2 oviposition substrate and processes: defat cotton-wool, smears the defat cotton-wool (only smearing, mealybug waxiness weighs about 0.7-0.85mg) of mealybug waxiness in the side of cotton-wool, cotton hereinafter referred to as wax.30 samples of each process.
10 days different substrates of record process the head number of the Cryptolaemus montrouzieri laid eggs in 2 periods of lower every day and corresponding egg laying amount continuously.Calculate spawning rate (female worm head number/30 × 100% laid eggs in period every day) in the different egg matrix treatments Cryptolaemus montrouzieri period, total amount (the ovum numbers of 30 Cryptolaemus montrouzieri collected in period every day) of laying eggs in the period, every day lay eggs total amount (every day take food laying eggs total amount and the lay eggs total amount sum of laying eggs period in, i.e. 24 hour lay eggs total amount in the period), every day is with the eggs on average amount (weight of laying eggs in this period/female borer population of laying eggs) in the period.
Table 4. different substrates processes lower Cryptolaemus montrouzieri at the per day egg laying amount (grain ovum/often female) taken food in the period
In table, data are meansigma methods ± standard error.Same column internal labeling same letter represent its under 0.05 level without significant difference, following table with.
Table 5. different substrates processes lower Cryptolaemus montrouzieri at the per day egg laying amount (grain ovum/often female) laid eggs in the period
Table 6 different substrates processes lower Cryptolaemus montrouzieri spawning rate in different periods
In table, A represents and takes food the period, and B represents lays eggs the period.
The result of table 4 and table 5 shows using Cotton Gossypii as oviposition substrate and cotton as oviposition substrate using wax, in continuous 10 days, eggs on average amount in the Cryptolaemus montrouzieri correspondence period is all without significant difference, and the eggs on average amount in the period of laying eggs is about 10, and the eggs on average amount in the period that takes food is only about 2.5, illustrate that laying eggs of Cryptolaemus montrouzieri almost concentrates on the period of laying eggs, in addition, when collecting this Ladybird egg, Cotton Gossypii can be used to replace wax cotton, namely replace mealybug ootheca (because wax cotton possesses the characteristic of mealybug ootheca) as the oviposition substrate of Cryptolaemus montrouzieri, make to collect laying eggs of this ladybug with the aforedescribed process.Although using Cotton Gossypii as oviposition substrate; the spawning rate of Cryptolaemus montrouzieri to be slightly less than using the cotton process (table 6) as oviposition substrate of wax; but wax cotton could prepare after needing extraction prey waxiness; Cotton Gossypii is used then more succinctly to facilitate; reduce the cost extracting prey waxiness simultaneously; meet and collect worm's ovum in a large number, the demand of biological control ladybug.
Test executes 4: the autotomy degree between larva
The ovum of the Cryptolaemus montrouzieri collected is placed in big plastic culture dish (9 × 1.5cm) and separately raises with mealybug, to obtain non-compatriot larva.When larvae development is to specifying after age (cast off a skin < 12 hours) for autotomy test.Test arranges 3 combinations: 1) low age (2 age) × low age (2 age)；2) low age (2 age) × advanced age (4 age)；3) advanced age (4 age) × advanced age (4 age).Similar with instar larvae body size, do not provide any food, respectively at the survival condition of 2 larvas of 12,24,36,48,60 hours observed and recordeds, 20 samples of each combination.Calculate the cumulative mortality of each 20 samples of combination.Result of the test is as follows:
Table 7: the autotomy mortality rate of larva
Shown by the result of the test of table 7, when there is no food, the autotomy of Cryptolaemus montrouzieri occurs mainly between high instar larvae and low instar larvae, autotomy cumulative mortality in 60h reaches 70%, and between high instar larvae, and the cumulative mortality that between low instar larvae, in 60h, autotomy causes is all not less than 30%, this may be primarily due to the larva body of Cryptolaemus montrouzieri by dense waxiness, can effectively suppress the injury that autotomy causes.And high instar larvae is due to the advantage of worm weight, even if low instar larvae has the protection of waxiness, it is also difficult to suppress the attack to them of the high instar larvae.But, because the low instar larvae of Cryptolaemus montrouzieri is generally all lived under prey, high instar larvae can be hidden well, so in sum, larva can adopt the mode raised in cages to raise without causing its mortality due to autotomy completely.
Test executes 5: the tolerance to cold of adult
Cryptolaemus montrouzieri in sprouting wings 1 day is placed in big plastic culture dish and is raised, and 25, every ware, every day, fresh mealybug was changed in timing, within 7 days, was placed on 10 ± 1 DEG C, and L:D=12:12 preserves in the growth cabinet of RH=70 ± 5%.Every 3 days later, take out 4 wares and be placed in the middle improvements of room temperature (26 ± 2 DEG C), and observe the survival condition of ladybug in ware, get the 21st day.Result of the test is table 8 such as:
Table 8: the survival rate of Cryptolaemus montrouzieri adult cryopreservation
In table 8 it is shown that Cryptolaemus montrouzieri is after 10 DEG C of cold preservations 6 days, survival rate still may be up to 94%, but just drops to 60% to the 9th day survival rate, afterwards along with the increase survival rate of cold preservation natural law is gradually lowered.So for the storage of Cryptolaemus montrouzieri adult with 10 DEG C, within 6 days, being that a period ratio is appropriate, cold preservation to provide fresh mealybug to take food by it after 6 days, supplements energy, is further continued for afterwards carrying out cold preservation, has reached long-time cold preservation and possessed the requirement of relatively high viability.
Claims (2)
1. a Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method, it is characterised in that comprise the following steps:
(1), mealybug cultivate: on the south melon and fruit implementation be host plant cultivate mealybug；
(2), ladybug is cultivated:
A. ovum period management: place defat cotton-wool in box raising, and smear to lay eggs and lure collection thing mealybug waxiness, the Cryptolaemus montrouzieri of emergence copulation is laid eggs on cotton-wool, collects cotton-wool standby or be placed in ventilative plastic casing, and cold preservation is standby when 14-17 DEG C；
B. larva is raised in cages: at temperature 24-28 DEG C, relative humidity 40-75%, under the condition of illumination 14 hour/day, the Fructus Cucurbitae moschatae with mealybug in step (1) is put on the pallet in dependent insect cage, the cotton-wool with Cryptolaemus montrouzieri ovum collected is received on Fructus Cucurbitae moschatae, after larvae hatch, on Fructus Cucurbitae moschatae, the quantity of mealybug is gradually increased, and ladybug of leaving takes food growth, until the mealybug on Fructus Cucurbitae moschatae takes food totally, only during surplus mealybug wax silk, put into the new Fructus Cucurbitae moschatae being covered with mealybug；
C, pupa time: temperature 24-28 DEG C, when relative humidity 40-75%, when Pediobius foveolatus length to 4 mature larva in latter stage in age, it is chosen in dependent insect cage and is placed in culture dish, and put into mealybug and ensure that its foodstuff is abundant, until pupating；
D. the emergence of adult: ladybug pupa is put into 24-28 DEG C, grows when relative humidity 40-75%, raises after emergence, and during raising, female-male proportion is 1:1, supplements mealybug simultaneously and supplements the nutrients, to guarantee that adult can normal growth and breeding so that it is lay eggs；
On the south described, melon and fruit implementation is that host plant cultivation mealybug concretely comprises the following steps: 24-28 DEG C, relative humidity 50-75%, under the condition of illumination 14 hour/day, choose the Fructus Cucurbitae moschatae that surface is comparatively coarse, clean with wet cloth, after drying, it is placed on shelf bracket, put up a bridge with the paper slip of width 1.5 ± 0.5cm, connect the Fructus Cucurbitae moschatae newly bought and cover with the Fructus Cucurbitae moschatae of mealybug, mealybug crawl on new host so that can shift, after 20-30 days, mealybug covers with when covering fresh Fructus Cucurbitae moschatae, just can be used for raising Pediobius foveolatus and adult, if period put up a bridge paper slip mouldy or be covered with mealybug secretion honeydew time, to change paper slip in time.
2. Cryptolaemus montrouzieri artificial breeding according to claim 1, conservation and propagation production method, it is characterized in that, described mealybug waxiness is to leach the mealybug with ootheca with volume fraction 99% aqueous acetone solution, removing the filtrate filter paper filtering after polypide and silk quality material, filtering residue is mealybug waxiness after drying.
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