CN112868604B - Method for controlling egg laying of propylaea japonica - Google Patents
Method for controlling egg laying of propylaea japonica Download PDFInfo
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- CN112868604B CN112868604B CN202110068818.7A CN202110068818A CN112868604B CN 112868604 B CN112868604 B CN 112868604B CN 202110068818 A CN202110068818 A CN 202110068818A CN 112868604 B CN112868604 B CN 112868604B
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- propylaea japonica
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for regulating egg laying of propylaea japonica, which comprises the following steps: (1) storing the male and female separately; (2) Storing at low temperature, namely placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃, setting the humidity to be 50-80%, and storing for 20-80 days under the condition of no illumination; (3) Supplementing nutrition, namely supplementing nutrition for male and female adults in the low-temperature storage process; (4) And (3) recovering spawning, pairing the stored male and female adults according to a proportion, and irradiating at a temperature of between 25 and 27 ℃ under an illumination L: d is 1697 h, 8h, and the egg laying can be recovered by feeding aphids for 3-5 days under the condition of humidity 60-80%. The method can flexibly regulate and control the oviposition period of the adult propylaea japonica as required, can keep the reproductive capacity of the adult propylaea japonica when aphids are deficient or the demand on the propylaea japonica population is less, and can restore the reproductive capacity of the adult propylaea japonica by changing the conditions when the demand is restored; by the regulation and control method, technical support can be provided for optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica.
Description
Technical Field
The invention belongs to the technical field of agriculture, in particular to a method for regulating and controlling egg laying of propylaea japonica,
background
The Propylaea japonica (Propylaea japonica Thungberg) belongs to Coleoptera, coccinelida and Propylaea, is widely distributed in most areas of China, can prey on various aphids on various crops, fruit trees and trees, eggs of certain lepidoptera and hemiptera insects, low-age larvae (nymphs), leaf mites and psylla adults and other pests, and is most likely to prey on aphids. And the propylaea japonica has the characteristics of large feed intake, more spawning, strong adaptability, more annual generations and the like, so the propylaea japonica is an important predatory natural enemy insect in agricultural production in China.
Because the breeding technology of the propylaea japonica by the artificial feed is not mature, the propylaea japonica fed by the artificial feed can complete the whole life cycle, but the breeding performance of the propylaea japonica fed by the artificial feed can not reach the quality of the population fed by natural prey. Therefore, the artificial breeding mode of the propylaea japonica still takes aphids as a main breeding method. In the actual breeding process, the situation that the coccinella japonica population cannot be subcultured due to aphid shortage or the situation that the coccinella japonica population wastes aphids and other related resources due to the reduction of the demand for the coccinella japonica often occurs.
Disclosure of Invention
The invention provides a method for regulating and controlling egg laying of propylaea japonica, which can solve the defects in the prior art.
The technical scheme of the invention is as follows:
a method for controlling eggs laying of propylaea japonica comprises the following steps: (1) Separately storing the male and female, and separately storing the male and female of the propylaea japonica to be regulated in different insect-breeding containers; (2) Storing at low temperature, namely placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃ and the humidity to be 50-80%, and storing under the condition without illumination; (3) Supplementing nutrition, namely supplementing nutrition for male and female adults in the low-temperature storage process; (4) And (3) recovering spawning, pairing the stored male and female adults according to a proportion, and irradiating at a temperature of between 25 and 27 ℃ under an illumination L: d is 1697 h, 8h, and the egg laying can be recovered by feeding aphids for 3-5 days under the condition of humidity 60-80%. The purposes of separate preservation and nutrition supplementation of the male and female are to improve the survival rate and the egg laying amount of adults.
Preferably, the propylaea japonica in the step (1) is propylaea japonica after eclosion of adults.
Preferably, in the step (1), the breeding density is 8-10 male or female worms stored in one liter of volume.
Preferably, 3-5L containers for the insects are used for preservation.
Preferably, the frequency of supplementing nutrients in step (3) is once every 3 to 5 days.
Preferably, the step (3) adopts sucrose solution or honey as nutrient solution.
Preferably, a 10% sucrose solution or 10% honey is used as the nutrient solution.
Preferably, in the step (4), the male and female adults are paired according to the ratio of 1.
Preferably, the time for low-temperature preservation in step (2) is 20-80 days.
These conditions are preferred in order to prolong the preservation time of the propylaea japonica as much as possible and ensure that the propylaea japonica has high survival rate under these conditions and the subsequent reproductive performance is not greatly influenced.
Compared with the prior art, the invention has the following beneficial effects:
the eggs laying control method of the propylaea japonica can flexibly control the egg laying period of propylaea japonica adults according to needs, can keep the fertility of adults when aphids are deficient or the demand on propylaea japonica populations is less, and can restore the fertility of adults by changing conditions when the demand is restored; by the regulation and control method, technical support can be provided for optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Detailed Description
The invention relates to a method for regulating egg laying of propylaea japonica, which can flexibly regulate the egg laying period of propylaea japonica adults according to requirements.
Since the propylaea japonica has no diapause phenomenon (Weijiahua, ran Bibi. Propylaea japonica research. Natural enemies of insects, 1983,5 (2): 89-93), the method is greatly different from the preservation method of propylaea japonica, harmonia axyridis and other ladybug with diapause characteristics. The latter can be induced into diapause state by changing conditions to realize long-term preservation, and has higher survival rate and reproductive performance after diapause is removed. The propylaea japonica can not be preserved for a long time by entering diapause, so the preservation technology is greatly different from that of diapause type propylaea japonica, and the regulation and control method is not suitable for propylaea japonica with the diapause period.
By the method, when aphids are deficient or the demand on the ladybug population is low, egg laying and population scale of the propylaea japonica can be flexibly controlled according to the demand under the condition of keeping the fertility of the adult, and the fertility of the adult is recovered when the demand is recovered, so that the aims of optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica are fulfilled.
The regulation and control method comprises the following steps:
1. selecting 100-150 healthy pupa of Hippodamia variegate, after pupa eclosion, adopting 3-5L insect-breeding boxes, storing eclosion male and female imagoes in the insect-breeding boxes separately, and placing 40-50 male or female Hippodamia variegate insects in each insect-breeding box;
2. putting the separately stored adult females and males of the propylaea japonica into an artificial climate box, and storing at 8-12 ℃ and 50-70% of humidity without illumination;
3. when the male and female adults are stored under the conditions, a 10% sucrose solution or a 10% honey solution is adopted every 3-5 days to supplement nutrition for the male and female adults;
4. when the amplification of the propylaea japonica population is required, the preserved male and female adults are subjected to amplification treatment according to the ratio of 1:1, and the ratio is matched, at the temperature of 25-27 ℃, the illumination L: d is 1697 h, 8h and humidity 60-70%, and the egg laying can be recovered after the aphid is fed for 3-5 days.
According to the method, the longest storage life of the adult male and female propylaea japonica is 20-80 days, and the reproductive performance is not obviously different from that of the population which is not stored at low temperature.
In this context, a range of values from one value to another is a general expression avoiding any recitation of all values in the range in the specification. Thus, recitation of a specific range of values herein includes any number within the range and any smaller range of values within the range, as if the range and smaller range were explicitly recited in the specification.
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In practice, the invention will be understood to cover all modifications and variations of this invention provided they come within the scope of the appended claims.
Example 1
Treatment 1: selecting 120 pupas of the propylaea japonica, after eclosion, respectively taking 40 pupas of male and female adults, and respectively storing the pupas in a 4.5L insect-raising box. And storing in incubator at 11 deg.C and 70% humidity without light. When the adult males and females are stored under the conditions, 10% of sucrose solution is adopted to supplement nutrition for every 3-5 days. After 40 days of preservation, the preserved survival male and female adults are paired, 20 pairs of the propylaea japonica are selected, and the temperature is 25 ℃, and the illumination L: d is 1697 h.
And (3) treatment 2: the storage temperature was 8 ℃ and other conditions were the same as in treatment 1.
And (3) treatment: the storage time was 80 days, and other conditions were the same as in treatment 1.
Control group: pairing newly-eclosized adult propylaea japonica which is not subjected to low-temperature preservation with male and female propylaea japonica, selecting 20 pairs of adult propylaea japonica, and irradiating at the temperature of 25 ℃ under the condition of light L: d is 1697 h, 8h, and the pea aphids are fed under the condition of 70% humidity, and the egg laying amount and the hatching rate of 14 days after pairing are recorded.
TABLE 1 Effect of different treatments on the survival rate and reproductive performance of propylaea japonica
As can be seen from the table, the adult propylaea japonica treated by the method 1 has the survival rate of 83%, and the adult propylaea japonica can still normally mate and lay eggs after being stored at 11 ℃ for 40 days and restored to the normal temperature, the total egg production amount reaches 188.67 eggs/female in 14 days, and the difference from the control is not significant. And the hatching rate of the eggs can also reach 80.91 percent. The level of the protein is equivalent to that of a control group. The survival rate of the adults treated by the treatment 2 (the adult worms are stored at 8 ℃ for 40 days) and the survival rate of the adults treated by the treatment 3 (the adult worms are stored at 11 ℃ for 80 days) are reduced compared with the survival rate of the adults treated by the treatment 1, but the survival rate still reaches more than 70%, the total egg laying amount in 14 days and the egg hatching rate are also lower than those of the adult worms treated by the treatment 1, and the difference is not obvious.
Therefore, by adopting the method of the embodiment, the artificial breeding of the propylaea japonica population scale can be flexibly controlled by adjusting the oviposition period of the adult propylaea japonica, the resources are reasonably configured, and the propagation cost is saved.
While the foregoing is directed to the preferred embodiment of the present invention, it is not intended to detail all of the same, and it is to be understood that such embodiment is merely illustrative of the present invention and is not to be considered as limiting the scope of the invention, which is limited only by the claims and their full scope and equivalents.
The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. In light of the above teachings, those skilled in the art will readily appreciate that the materials and their equivalents, the processes and their equivalents, and the upper and lower limits and ranges of the parameters of the materials and processes, as recited and exemplified in the present disclosure, can all practice the present disclosure, and the examples are not intended to be exhaustive.
Claims (4)
1. A method for regulating and controlling eggs laying of propylaea japonica is characterized by comprising the following steps:
(1) Separate preservation of male and female
Separately storing the female and male ladybug of the propylaea japonica to be regulated in different larva breeding containers;
(2) Low temperature preservation
Placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃ and the humidity to be 50-80%, and storing under the condition without illumination;
(3) Supplementing nutrition
In the process of low-temperature storage, the male and female adults are given nutrition supplement;
(4) Resume spawning
Pairing the preserved male and female adults according to the proportion of 1: d is 1697 h, 8h, and the egg laying can be recovered by feeding aphids for 3-5 days under the condition of humidity 60-80%;
in the step (1), 8-10 male worms or female worms are stored in one liter of volume of culture density; the frequency of supplementing nutrition in the step (3) is once every 3 to 5 days, and a sucrose solution or honey is used as a nutrient solution;
if the demand of the propylaea japonica is deficient, preserving the propylaea japonica at low temperature, and if the demand of the propylaea japonica is increased, recovering the laying;
wherein the propylaea japonica in the step (1) is propylaea japonica after eclosion of adults.
2. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: preserving with 3-5L insect container.
3. The method for regulating eggs laying of propylea japonica according to claim 1, characterized in that: a10% sucrose solution or 10% honey is used as the nutrient solution.
4. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: the low-temperature preservation time in the step (2) is 20-80 days.
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| JP2002058391A (en) * | 2000-08-21 | 2002-02-26 | Kazuyasu Kubota | Artificial wood for oviposition of stag beetle or the like |
| JP2008154459A (en) * | 2006-12-20 | 2008-07-10 | Irago Institute Co Ltd | Method for accelerating maturation of eel |
| KR101178392B1 (en) * | 2011-02-24 | 2012-08-30 | 부경대학교 산학협력단 | Apparatuses and method for artificial seedling production of marine polychaete |
| CN102771445B (en) * | 2012-08-07 | 2014-03-26 | 中国农业科学院植物保护研究所 | Diapause cancellation method of natural enemy insect |
| CN103503838B (en) * | 2013-10-15 | 2015-05-20 | 广东省农业科学院植物保护研究所 | Method for rearing litchi fruit borers |
| CN104396881A (en) * | 2014-09-19 | 2015-03-11 | 中山大学 | Propylaea japonica artificial breeding, breed conservation and propagation production method |
| CN104365557B (en) * | 2014-09-19 | 2016-07-06 | 中山大学 | A kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method |
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| CN109548754B (en) * | 2018-12-06 | 2022-04-29 | 甘肃农业大学 | Low-temperature storage method of adult ladybug |
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