CN112868604A - Method for controlling egg laying of propylaea japonica - Google Patents
Method for controlling egg laying of propylaea japonica Download PDFInfo
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- CN112868604A CN112868604A CN202110068818.7A CN202110068818A CN112868604A CN 112868604 A CN112868604 A CN 112868604A CN 202110068818 A CN202110068818 A CN 202110068818A CN 112868604 A CN112868604 A CN 112868604A
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- propylaea japonica
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for regulating egg laying of propylaea japonica, which comprises the following steps: (1) the male and female parts are separately preserved; (2) storing at low temperature, namely placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃, setting the humidity to be 50-80%, and storing for 20-80 days under the condition of no illumination; (3) supplementing nutrition, namely supplementing nutrition for male and female adults in the low-temperature storage process; (4) and (3) recovering spawning, pairing the stored male and female adults according to a proportion, and irradiating at a temperature of between 25 and 27 ℃ under an illumination L: d is 16h, 8h, and the egg can be recovered by feeding aphids for 3-5 days under the condition of 60-80% of humidity. The method can flexibly regulate and control the oviposition period of the adult propylaea japonica as required, can keep the reproductive capacity of the adult propylaea japonica when aphids are deficient or the demand on the propylaea japonica population is less, and can restore the reproductive capacity of the adult propylaea japonica by changing the conditions when the demand is restored; by the regulation and control method, technical support can be provided for optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica.
Description
Technical Field
The invention belongs to the technical field of agriculture, in particular to a method for regulating and controlling egg laying of propylaea japonica,
background
The Propylaea japonica (propyllaea japonica Thungberg) belongs to Coleoptera (Coleoptera), the Propylaea (Coccinelideae) and Propylaea (propyllaea), is widely distributed in most areas of China, can prey on various aphids on various crops, fruit trees and woods, eggs of certain lepidoptera and hemiptera insects, low-age larvae (nymphs), leaf mites and psylla adults and other various pests, and is most favorable for preying on the aphids. And the propylaea japonica has the characteristics of large feed intake, more spawning, strong adaptability, more annual generations and the like, so the propylaea japonica is an important predatory natural enemy insect in agricultural production in China.
The breeding technique of the propylaea japonica by the artificial feed is not mature, so that the propylaea japonica fed by the artificial feed can complete the whole life
The period is long, but the reproductive performance of the wild variety can not reach the quality of the population fed with natural prey. Therefore, the artificial breeding mode of the propylaea japonica still takes aphids as a main breeding method. In the actual breeding process, the situation that the coccinella japonica population cannot be subcultured due to aphid shortage or the situation that the coccinella japonica population wastes aphids and other related resources due to the reduction of the demand for the coccinella japonica often occurs.
Disclosure of Invention
The invention provides a method for controlling egg laying of propylaea japonica, which can solve the defects in the prior art.
The technical scheme of the invention is as follows:
a method for controlling eggs laying of propylaea japonica comprises the following steps: (1) separately storing the male and female, and separately storing the male and female of the propylaea japonica to be regulated in different insect-breeding containers; (2) storing at low temperature, namely placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃ and the humidity to be 50-80%, and storing under the condition without illumination; (3) supplementing nutrition, namely supplementing nutrition for male and female adults in the low-temperature storage process; (4) and (3) recovering spawning, pairing the stored male and female adults according to a proportion, and irradiating at a temperature of between 25 and 27 ℃ under an illumination L: d is 16h, 8h, and the egg can be recovered by feeding aphids for 3-5 days under the condition of 60-80% of humidity. The purposes of separate preservation and nutrition supplementation of the male and female are to improve the survival rate and the egg laying amount of adults.
Preferably, the propylaea japonica in the step (1) is propylaea japonica after eclosion of adults.
Preferably, in the step (1), the breeding density is 8-10 male or female insects stored in one liter of volume.
Preferably, 3-5L containers for the insects are used for preservation.
Preferably, the frequency of supplementing nutrients in step (3) is once every 3 to 5 days.
Preferably, the step (3) adopts sucrose solution or honey as nutrient solution.
Preferably, a 10% sucrose solution or 10% honey is used as the nutrient solution.
Preferably, in the step (4), the male and female adults are paired according to a ratio of 1: 1.
Preferably, the time for low-temperature preservation in step (2) is 20-80 days.
These conditions are preferred in order to prolong the storage time of the propylaea japonica as much as possible and ensure that the propylaea japonica has high survival rate under the conditions and does not have great influence on the subsequent reproductive performance.
Compared with the prior art, the invention has the following beneficial effects:
the egg laying regulation and control method of the propylaea japonica can flexibly regulate and control the egg laying period of the propylaea japonica adults according to needs, can keep the fertility of the adults when aphids are deficient or the demand on the propylaea japonica populations is less, and can restore the fertility of the adults by changing the conditions when the demand is restored; by the regulation and control method, technical support can be provided for optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Detailed Description
The invention relates to a method for regulating egg laying of propylaea japonica, which can flexibly regulate the egg laying period of propylaea japonica adults according to requirements.
Since the propylaea japonica has no diapause phenomenon (Weijiahua, ran Bibi. propylaea japonica research. natural enemies of insects 1983,5(2):89-93), the method has a great difference from the preservation method of the propylaea japonica, the harmonia axyridis and other propylaea japonica with diapause characteristics. The latter can be induced into diapause state by changing conditions to realize long-term preservation, and has higher survival rate and reproductive performance after diapause is removed. The propylaea japonica can not be preserved for a long time by entering diapause, so the preservation technology is greatly different from that of diapause type propylaea japonica, and the regulation and control method is not suitable for propylaea japonica with the diapause period.
By the method, when aphids are deficient or the demand on the ladybug population is low, egg laying and population scale of the propylaea japonica can be flexibly controlled according to the demand under the condition of keeping the fertility of the adult, and the fertility of the adult is recovered when the demand is recovered, so that the aims of optimizing resource allocation and saving cost in the large-scale production process of the propylaea japonica are fulfilled.
The regulation and control method comprises the following steps:
1. selecting 150 healthy propylaea japonica pupae with 100-;
2. putting the separately stored adult female and male propylaea japonica into an artificial climate box, and storing at 8-12 deg.C and 50-70% humidity without illumination;
3. when the male and female adults are stored under the conditions, a 10% sucrose solution or a 10% honey solution is adopted every 3-5 days to supplement nutrition for the male and female adults;
4. when the amplification of the propylaea japonica population is required, the preserved male and female adults are subjected to amplification treatment according to the ratio of 1:1, and the ratio is matched, at the temperature of 25-27 ℃, the illumination L: d is 16h, 8h, and the egg can be recovered by feeding aphids for 3-5 days under the condition of 60-70% of humidity.
According to the method, the longest storage life of the adult male and female propylaea japonica is 20-80 days, and the reproductive performance is not obviously different from that of the population which is not stored at low temperature.
In this context, a range of values from one value to another is a general expression avoiding any recitation of all values in the range in the specification. Thus, recitation of a range of values herein is intended to encompass any value within the range and any smaller range defined by any value within the range, as if the range and smaller range were explicitly recited in the specification.
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In practice, the invention will be understood to cover all modifications and variations of this invention provided they come within the scope of the appended claims.
Example 1
Treatment 1: selecting 120 pupas of the propylaea japonica, after eclosion, respectively taking 40 pupas of male and female adults, and respectively storing the pupas in a 4.5L insect-raising box. And storing in incubator at 11 deg.C and 70% humidity without light. When the adult males and females are stored under the conditions, 10% of sucrose solution is adopted to supplement nutrition for every 3-5 days. After 40 days of preservation, the preserved survival male and female adults are paired, 20 pairs of the paired propylaea japonica are selected, and the temperature is 25 ℃, and the illumination L: d is 16h:8h, the pea aphids are fed under the condition of 70% humidity, the egg laying amount and the hatching rate 14 days after pairing are recorded, and the results are shown in a table 1.
And (3) treatment 2: the storage temperature was 8 ℃ and other conditions were the same as in treatment 1.
And (3) treatment: the storage time was 80 days, and other conditions were the same as in treatment 1.
Control group: pairing newly-emerged adult propylaea japonica which is not preserved at low temperature, selecting 20 pairs of adult propylaea japonica, and irradiating at 25 ℃ under the condition of light L: d is 16h, 8h, feeding the pea aphids under the condition of 70% humidity, and recording the egg laying amount and the hatching rate 14 days after pairing.
TABLE 1 Effect of different treatments on Hippodamia japonica survival and reproductive Performance
As can be seen from the table, the adult propylaea japonica treated by the method 1 has the survival rate of 83%, and the adult propylaea japonica can still normally mate and lay eggs after being stored at 11 ℃ for 40 days and restored to the normal temperature, the total egg production amount reaches 188.67 eggs/female in 14 days, and the difference from the control is not obvious. And the hatching rate of eggs can also reach 80.91 percent. The level of the protein is equivalent to that of a control group. The survival rate of the adults treated by the treatment 2 (the adult worms are stored at 8 ℃ for 40 days) and the survival rate of the adults treated by the treatment 3 (the adult worms are stored at 11 ℃ for 80 days) are reduced compared with the survival rate of the adults treated by the treatment 1, but the survival rate still reaches more than 70%, the total egg laying amount in 14 days and the egg hatching rate are also lower than those of the adult worms treated by the treatment 1, and the difference is not.
Therefore, by adopting the method of the embodiment, the artificial breeding of the propylaea japonica population scale can be flexibly controlled by adjusting the oviposition period of the adult propylaea japonica, the resources are reasonably configured, and the propagation cost is saved.
While the foregoing is directed to the preferred embodiment of the present invention, it is not intended to detail all of the same, and it is to be understood that such embodiment is merely illustrative of the present invention and is not to be considered as limiting the scope of the invention, which is limited only by the claims and their full scope and equivalents.
The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. In light of the above teachings, those skilled in the art will readily appreciate that the materials and their equivalents, the processes and their equivalents, as listed or exemplified herein, are capable of performing the invention in any of its several forms, and that the upper and lower limits of the parameters of the materials and processes, and the ranges of values between these limits are not specifically enumerated herein.
Claims (9)
1. A method for controlling eggs laying of propylaea japonica is characterized by comprising the following steps:
(1) separate preservation of male and female
Separately storing the female and male ladybug of the propylaea japonica to be regulated in different larva breeding containers;
(2) low temperature preservation
Placing the insect culture container in the step (1) into a culture device, setting the storage temperature to be 8-12 ℃ and the humidity to be 50-80%, and storing under the condition without illumination;
(3) supplementing nutrition
In the process of low-temperature storage, the male and female adults are given nutrition supplement;
(4) resume spawning
Pairing the stored male and female adults according to a proportion, and irradiating at a temperature of between 25 and 27 ℃ under an illumination L: d is 16h, 8h, and the egg can be recovered by feeding aphids for 3-5 days under the condition of 60-80% of humidity.
2. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: the propylaea japonica in the step (1) is the propylaea japonica after eclosion of adults.
3. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: in the step (1), 8-10 male worms or female worms are stored in one liter of volume of culture density.
4. The method for regulating eggs laying of propylaea japonica according to claim 3, wherein: preserving with 3-5L insect container.
5. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: the frequency of supplementing nutrition in the step (3) is once every 3 to 5 days.
6. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: and (3) adopting a sucrose solution or honey as a nutrient solution.
7. The method for regulating eggs laying of propylaea japonica according to claim 6, wherein: a10% sucrose solution or 10% honey is used as the nutrient solution.
8. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: and (4) pairing the male and female adults according to the ratio of 1: 1.
9. The method for regulating eggs laying of propylaea japonica according to claim 1, wherein: the low-temperature preservation time in the step (2) is 20-80 days.
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Cited By (1)
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| CN114794027A (en) * | 2022-05-15 | 2022-07-29 | 中国医学科学院药用植物研究所云南分所 | Indoor propagation method for cyrtorhinus lividipennis |
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| CN114794027A (en) * | 2022-05-15 | 2022-07-29 | 中国医学科学院药用植物研究所云南分所 | Indoor propagation method for cyrtorhinus lividipennis |
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