CN105325377B - The biological control method of minute pirate bugs - Google Patents
The biological control method of minute pirate bugs Download PDFInfo
- Publication number
- CN105325377B CN105325377B CN201510864907.7A CN201510864907A CN105325377B CN 105325377 B CN105325377 B CN 105325377B CN 201510864907 A CN201510864907 A CN 201510864907A CN 105325377 B CN105325377 B CN 105325377B
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- Prior art keywords
- adult
- similis
- nymph
- black bean
- minute pirate
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- 241001635552 Anthocoridae Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241000819999 Nymphes Species 0.000 claims abstract description 29
- 241001425390 Aphis fabae Species 0.000 claims abstract description 17
- 241000927584 Frankliniella occidentalis Species 0.000 claims abstract description 15
- 241001414989 Thysanoptera Species 0.000 claims abstract description 6
- 241000576418 Jasminum nudiflorum Species 0.000 claims description 13
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 12
- 235000021332 kidney beans Nutrition 0.000 claims description 12
- 241000238631 Hexapoda Species 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 240000006677 Vicia faba Species 0.000 claims description 7
- 235000010749 Vicia faba Nutrition 0.000 claims description 7
- 235000002098 Vicia faba var. major Nutrition 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 241000382353 Pupa Species 0.000 claims description 6
- 125000006850 spacer group Chemical group 0.000 claims description 6
- 240000008620 Fagopyrum esculentum Species 0.000 claims description 4
- 235000009419 Fagopyrum esculentum Nutrition 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000035784 germination Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000010903 husk Substances 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 241001124076 Aphididae Species 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 230000017448 oviposition Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 241001635529 Orius Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the biological control method of minute pirate bugs.Frankliniella occidentalis and black bean aphid are combined and feed Similis by the present invention, take full advantage of the fireballing advantage of black bean aphid population growth, the characteristics of make use of Frankliniella occidentalis to improve Similis incubates nymph survival rate and adult reproductive capacity at the same time, Frankliniella occidentalis is fed by Similis incubates nymph and improves survival rate, black bean aphid is fed with reference to 25 age nymphs to reduce feeding cost, and then the purpose of low cost, efficiently breeding Similis.The breeding technology flow of a set of suitable biological control minute pirate bugs is produced, the numerous Similis of largely expansion that can be rapidly and efficiently, for pests such as field control thrips, tetranychid, aphids.
Description
Technical field
The present invention relates to insect science technical field, the biological control method of especially a kind of minute pirate bugs.
Background technology
Similis（Orius similisZheng）Belong to Semiptera（Hemiptera）Anthocoridae
（Anthocoridae）Minute pirate bugs category（Orius）, it is distributed widely in south and saves more, field often can see such natural enemy insect
Be active in above the crops such as fruit tree, vegetables, horticulture and flower and prey on various mini pests, including thrips, tetranychid, aphid, aleyrodid with
And the ovum and low instar larvae of more Lepidopterous, Diptera pest, great development prospect.The states such as Canada, Holland, the U.S., Japan
Family successfully carries out merchandized handling to minute pirate bugs, and the large-scale production of the minute pirate bugs in China relatively lags behind.
The content of the invention
The purpose of the present invention is：A kind of biological control method of minute pirate bugs is provided, feeding cost is low, and reproductive efficiency is high,
It is adapted to biological control Similis.
What the present invention was realized in：The biological control method of minute pirate bugs, includes the following steps：
1）The raising of Frankliniella occidentalis：
a）Adult stage：Clean fresh kidney beans are put into the insect box of raising adult, replace once raise adult daily
Fresh kidney beans；
b）Nymph：The fresh kidney beans changed in adult box are collected, is put into new insect box, is observed daily, in due course
Replace and add fresh beanpod；
c）Prepupa and pupa：Until thrips nymph is changed into prepupa and pupa, motionless insect box, is also changed without fresh kidney beans, to its into
Row dark processing is until turn into adult；
2）The raising of black bean aphid：When first using Seed soaking 24 small before Broad Bean Seeds are sowed, after cleaning up, directly it is sown into
In seedling-growing container, when germination is unearthed 1.5-3cm, black bean aphid adult is accessed, is allowed to breed, it is stand-by；
3）Similis adult and the raising of nymph：
d）Adult stage：Prepare the container of an open top, and spacer medium is laid with bottom, launch winter jasmine successively
Tender stem, Frankliniella occidentalis nymph and Similis adult, with 120 mesh gauzes by container closure, are placed above in gauze and use quality
The cotton balls that the hydromel that concentration is 5% soaked, replaces winter jasmine tender stem and supplements prey and minute pirate bugs adult daily；
e）The nymph stage：By step d）The winter jasmine tender stem of middle taking-up is put into foster worm bottle, the winter jasmine in each foster worm bottle
Flower tender stem includes 130-170 ovum, and the broad bean seedling of 3-4 plants of 4-5cm high is equipped with foster worm bottle, and accesses access advanced age black bean aphid
7-8 heads, after discovery has black bean aphid nymph to hatch, then add 2 age nymph 300-400 heads of Frankliniella occidentalis, continuously addition 2 days, and 15
Start to collect the Similis adult to sprout wings after it.
The environmental condition of whole feeding process, 25-27 DEG C of temperature, humidity 60-80%, illumination 16L:8D.
The spacer medium is buckwheat shell or hardboard.
Frankliniella occidentalis and black bean aphid are combined and feed Similis by the present invention, take full advantage of the increasing of black bean aphid population
Long fireballing advantage, while make use of Frankliniella occidentalis to improve Similis incubates nymph survival rate and adult breeding
The characteristics of power, feed Frankliniella occidentalis by Similis incubates nymph and improve survival rate, black bean aphid is fed with reference to 2-5 ages nymph
To reduce feeding cost, and then the purpose of low cost, efficiently breeding Similis.Produce a set of suitable biological control
The breeding technology flow of minute pirate bugs, the numerous Similis of largely expansion that can be rapidly and efficiently, for field control thrips, tetranychid,
The pests such as aphid.
Embodiment
The embodiment of the present invention 1：The biological control method of minute pirate bugs, includes the following steps：
1）The raising of Frankliniella occidentalis：
a）Adult stage：Clean fresh kidney beans are put into the insect box of raising adult, replace once raise adult daily
Fresh kidney beans；
b）Nymph：The fresh kidney beans changed in adult box are collected, is put into new insect box, is observed daily, in due course
Replace and add fresh beanpod；
c）Prepupa and pupa：Until thrips nymph is changed into prepupa and pupa, motionless insect box, is also changed without fresh kidney beans, to its into
Row dark processing is until turn into adult；
2）The raising of black bean aphid：When first using Seed soaking 24 small before Broad Bean Seeds are sowed, after cleaning up, directly it is sown into
In seedling-growing container, when germination is unearthed 1.5-3cm, black bean aphid adult is accessed, is allowed to breed, it is stand-by；
3）Similis adult and the raising of nymph：
d）Adult stage：Launch winter jasmine tender stem, the Frankliniella occidentalis of 10 long 10cm or so successively in imago breeding bottle
Nymph 1000 or so and 50 pairs of Similis adults, spacer medium is laid with raising bottom of bottle portion, isolates in the present embodiment and is situated between
Matter selects buckwheat shell, with 120 mesh gauzes by container closure, is placed above in gauze and was soaked with the hydromel that mass concentration is 5%
Cotton balls, replace winter jasmine tender stem daily and simultaneously supplement prey and minute pirate bugs adult；
e）The nymph stage：The winter jasmine tender stem of taking-up is put into foster worm bottle, winter jasmine tender stem includes 130-170 ovum,
The broad bean seedling of 3-4 plants of 4-5cm high is equipped with foster worm bottle, and accesses access advanced age black bean aphid 7-8 head, discovery has Similis
After nymph hatches, then 2 age nymph 300-400 heads of Frankliniella occidentalis are added, continuous addition starts to collect what is sprouted wings after 2 days, 15 days
Similis adult.
The environmental condition of whole feeding process, 25-27 DEG C of temperature, humidity 60-80%, illumination 16L:8D.
In the present embodiment, container and foster worm bottle used in Similis adult and nymph stage using 5L plastic bottles come
Make, a long * wide is respectively opened in its both sides：The ventilation hole of 11cm*8cm, is sealed with 120 mesh gauzes；Built-in cardboard box fragment or
Buckwheat shell etc. is used as spacer medium.
And the broad bean seedling root placed in the foster worm bottle used in the nymph stage is wrapped up with immersion cotton and preservative film.
Inventor uses the above method in December, 2014 in Guizhou Plant Protection Institute's intelligence growth cabinet
The breeding experiment 1 of Similis is carried out, adult pick-up rate has reached 56.4% and has been shown in Table 1.
Experiment 1
The oviposition substrate winter jasmine tender stem of taking-up is subjected to microscopy, record in oviposition substrate ovum amount, ovum will have been counted
The oviposition substrate of amount is put into nymph raising bottle, and ovum amount is controlled at 150 or so（130 ~ 170）, it is repeated 6 times altogether, specifically
Experimental detail is shown in embodiment
Embodiment 1,3）.
Conclusion:Using this set minute pirate bugs method for breeding, minute pirate bugs adult pick-up rate average value is 56.4%, and method is simple, into
This is cheap, can greatly improve the pick-up rate of minute pirate bugs adult.
Claims (3)
1. a kind of biological control method of minute pirate bugs, it is characterised in that：Include the following steps：
1）The raising of Frankliniella occidentalis：
a）Adult stage：Clean fresh kidney beans are put into the insect box of raising adult, replace the fresh kidney beans for once raising adult daily；
b）Nymph：The fresh kidney beans changed in adult box are collected, is put into new insect box, is observed daily, are replaced in due course
With the fresh beanpod of addition；
c）Prepupa and pupa：Until thrips nymph is changed into prepupa and pupa, motionless insect box, is also changed without fresh kidney beans, it is carried out black
Dark treatment is until turn into adult；
2）The raising of black bean aphid：When first using Seed soaking 24 small before Broad Bean Seeds are sowed, after cleaning up, nursery is directly sown into
In basin, when germination is unearthed 1.5-3cm, black bean aphid adult is accessed, is allowed to breed, it is stand-by；
3）Similis adult and the raising of nymph：
d）Adult stage：Prepare the container of an open top, and spacer medium is laid with bottom, it is tender to launch winter jasmine successively
Stem, Frankliniella occidentalis nymph and Similis adult, with 120 mesh gauzes by container closure, are placed above dense with quality in gauze
The cotton balls soaked for 5% hydromel is spent, winter jasmine tender stem is replaced daily and supplements prey and minute pirate bugs adult；
e）The nymph stage：By step d）The winter jasmine tender stem of middle taking-up is put into foster worm bottle, and the winter jasmine in each foster worm bottle is tender
Stem includes 130-170 ovum, and the broad bean seedling of 3-4 plants of 4-5cm high is equipped with foster worm bottle, and accesses access advanced age black bean aphid 7-8
Head, after discovery has black bean aphid nymph to hatch, then adds 2 age nymph 300-400 heads of Frankliniella occidentalis, after continuously adding 2 days, 15 days
Start to collect the Similis adult to sprout wings.
2. the biological control method of minute pirate bugs according to claim 1, it is characterised in that：The environment of whole feeding process
Condition, 25-27 DEG C of temperature, humidity 60-80%, illumination 16L:8D.
3. the biological control method of minute pirate bugs according to claim 1, it is characterised in that：The spacer medium is buckwheat
Wheat husk or hardboard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510864907.7A CN105325377B (en) | 2015-12-01 | 2015-12-01 | The biological control method of minute pirate bugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510864907.7A CN105325377B (en) | 2015-12-01 | 2015-12-01 | The biological control method of minute pirate bugs |
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| Publication Number | Publication Date |
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| CN105325377A CN105325377A (en) | 2016-02-17 |
| CN105325377B true CN105325377B (en) | 2018-04-20 |
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| CN201510864907.7A Expired - Fee Related CN105325377B (en) | 2015-12-01 | 2015-12-01 | The biological control method of minute pirate bugs |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106305628A (en) * | 2016-08-22 | 2017-01-11 | 北京阔野田园生物技术有限公司 | Method for scale propagation of amphiareus obscuriceps |
| CN107568170B (en) * | 2017-09-30 | 2020-09-15 | 贵州省烟草公司遵义市公司 | Large-scale artificial feeding method for lugworm bugs |
| CN110295198A (en) * | 2019-06-26 | 2019-10-01 | 华中农业大学 | A kind of method and its application that evaluation external source dsRNA influences Similis |
| CN110447604B (en) * | 2019-09-17 | 2020-07-28 | 中国农业科学院植物保护研究所 | Method for improving feeding effect of arma chinensis by adding alternative host plant |
| CN111493031A (en) * | 2020-05-18 | 2020-08-07 | 河南省农业科学院烟草研究所 | Method for reducing mortality rate of middle-aged and low-aged nymphs in raising orius sauteri |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TW384207B (en) * | 1997-08-20 | 2000-03-11 | Fumakilla Ltd | Piezoelectric chemical-liquid atomizer apparatus and method for repelling or eliminating harmful organism |
| CN101167533B (en) * | 2007-11-30 | 2012-03-21 | 河南省农业科学院植物保护研究所 | Bait for raising orius sauteri and method for group raising |
| CN102293182B (en) * | 2011-07-18 | 2013-04-03 | 山东省农业科学院植物保护研究所 | Orius sauteri raising method |
| CN102640729B (en) * | 2012-04-06 | 2014-02-26 | 山东省农业科学院植物保护研究所 | Method for breeding frankliniella occidentalis |
| CN104222006B8 (en) * | 2013-06-21 | 2016-12-21 | 中国农业科学院植物保护研究所 | A kind of artificial a large amount of methods producing minute pirate bugs |
| CN103704176B (en) * | 2013-11-29 | 2015-05-06 | 贵州大学 | Orius strigicollis raising method |
| CN104012472B (en) * | 2014-03-12 | 2016-08-17 | 云南农业大学 | The indoor feeding method of thrips |
| CN104938429B (en) * | 2015-06-30 | 2017-04-05 | 山东省农业科学院植物保护研究所 | A kind of method for raising Orius |
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