CN102640729B - Method for breeding frankliniella occidentalis - Google Patents
Method for breeding frankliniella occidentalis Download PDFInfo
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- CN102640729B CN102640729B CN201210099765.6A CN201210099765A CN102640729B CN 102640729 B CN102640729 B CN 102640729B CN 201210099765 A CN201210099765 A CN 201210099765A CN 102640729 B CN102640729 B CN 102640729B
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Abstract
The invention relates to the field of plant protection and the technology of insect cultivation, and in particular relates to a method for breeding frankliniella occidentalis by using water planting soya bean seedlings as fodder. According to the conventional method, kidney beans are used as the fodder, while the soya bean seedlings are used for feeding the frankliniella occidentalis according to the method, and therefore, the defect of feeding the frankliniella occidentalis caused by the problem of the fodder per se is changed. As the water planting soya bean seedlings have a short growth cycle, a low production environment requirement and low production cost and can be supplied throughout the year, according to the method for breeding the frankliniella occidentalis, the fodder basis for feeding the frankliniella occidentalis in a large scale can be provided and an excellent feeding effect can be realized.
Description
Technical field
The invention belongs to plant protection field and cultured insect technology, relate to a kind of raising method of Frankliniella occidentalis.
Background technology
Frankliniella occidentalis claims again alfalfa thrips, belongs to thrips Thysanoptera, Thripidae Thtipidae.This worm originates in North America, and first nineteen fifty-five is found in Kauai, Hawaii, be once the modal a kind of thrips of California, USA.After the eighties in 20th century, become surging kind, gradually to external expansion.Frankliniella occidentalis fertility is very strong, individual tiny, has invisiblely, and general field control is difficult to effective control.The host plant of Frankliniella occidentalis is very extensive, 200 various plants of at present known You66Ge section, open country plant mainly contains tomato, celery, pea, onion, apple, grape etc., greenhouse vegetable and flowers mainly contain pimento, cucumber, rose, orchid, African violet etc., and its host types is along with Frankliniella occidentalis is constantly diffused in continuous increase.1997, Frankliniella occidentalis was listed the dangerous venereal disease of the potential inward plant quarantine of China < <, worm, weeds list > > in; 2003, reported first was found Frankliniella occidentalis in the greenhouse of Beijing suburb, and all there is the report of Frankliniella occidentalis in Yunnan, Zhejiang, Shandong now.
Due to deepening continuously and the development of control of insect new technology of entomology research, need to be supplied in ten thousand more than one hundred million standardized insects of physiological standard, artificial feeding insect can make insect grow neat, physiology is consistent, therefore, the research work of insect feedstuff becomes one of basic fundamental in entomology research and control of insect new technology.At present, to Frankliniella occidentalis indoor feeding, mainly rely on fresh vegetables as kidney bean, pimento etc., oneself plants these vegetables has increased foster worm cost, and commercial vegetables are owing to not knowing medicining condition, experiment for some pesticide resistance detections and medicament resistance screening can impact, therefore need to find a kind ofly not controlled by season, the feed that cost the is low Frankliniella occidentalis of feeding on a large scale meets the requirement of scientific research to insect population radix.
Summary of the invention
The problems that exist for existing Frankliniella occidentalis artificial feeding, in order to better meet the needs of scientific experiment, the extensive raising of development Frankliniella occidentalis, inventor is through long-term experiment, providing a kind of adopts indoor water planting bean seedlings as feed, to raise the method for Frankliniella occidentalis, this method adopts the vegetables such as kidney bean as the method for feed before changing, and has changed due to these feed built-in problems and Frankliniella occidentalis is raised to the drawback of bringing.Utilize water planting bean seedlings growth cycle short, it is low that production environment requires, throughout the year can long run supply, and the feature that production cost is low, for the extensive raising of Frankliniella occidentalis provides the basis on feed, and the effect of feeding is splendid.
Concrete technical scheme of the present invention is: adopt water planting bean seedlings to raise Frankliniella occidentalis, wherein, adopt and see that the soya bean seedling that light is processed 3-4 days carries out feeding of Frankliniella occidentalis nymph and adult, adopt the new moyashi that has no light processing as oviposition substrate.
Concrete steps are as follows:
(1) water planting of soya bean seedling: select full seed, without insect population, the soya bean that goes mouldy, be placed in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading cultivation, when bean sprouts is germinateed to 2-4cm, in water planting liquid, add paclobutrazol solution, when bean sprouts grows to 9-12cm, shift out bean sprout growing machine, carry out fluorescent lamp and see light processing, grow into soya bean seedling;
(2) raising of Frankliniella occidentalis: the moyashi with Frankliniella occidentalis ovum is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, when ovum hatching has nymph to occur, resting worm cylinder is transferred in the box for feeding pests that is placed with the fresh bean seedlings of whole strain, box for feeding pests is placed in insectary, keep 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D feeds to Frankliniella occidentalis nymph and adult.
First select full seed, without insect population, the soya bean that goes mouldy, be placed on the pallet that has osculum in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading and cultivate, should be in time in germination process rotten beans, the soya bean that do not germinate not in time be chosen, in case mouldy and cause that other bean seedlings are rotten.
Inner at pulp while laying eggs due to Frankliniella occidentalis, therefore the Frankliniella occidentalis medium of laying eggs need to have thicker pulp and higher water content, and be difficult in a short time withered, once otherwise medium dehydration, thrips is just not easy survival, therefore the moyashi that the present invention adopts is in step (1), to see that it is 2-4cm moyashi that light is processed previous length, and this moyashi pulp is very thick, moisture is high, is suitable as oviposition substrate.In order to make moyashi more be conducive to Frankliniella occidentalis, lay eggs, when bean sprouts is germinateed to 2-4cm, in water planting liquid, add paclobutrazol solution.Paclobutrazol is a kind of interior suction, broad-spectrum plant growth retardent, conditioning agent, can be absorbed by the root of plant, stem, leaf, can suppress the growth of plant shoot, reduce plant height, dwindle leaf area, alleviate dry matter, but stem is slightly increased, adding after paclobutrazol solution the concentration of paclobutrazol in whole water planting liquid is 10-20ppm, this concentration can severe inhibition root growth, but it is too fast to suppress bean seedlings growth rate, stem is increased thick, the plumpness that blade becomes is healthy and strong, thereby be conducive to Frankliniella occidentalis, lays eggs.
Because Frankliniella occidentalis is inhaled phytophagous blade and fruit, so feed, adopt soya bean seedling when Frankliniella occidentalis nymph and adult, and bean sprouts grows up to bean seedlings, blade amount also increases, thereby reduced cost, therefore, in experimentation, when bean sprouts grows to 9-12cm, shifted out bean sprout growing machine, carry out fluorescent lamp and see light processing, it is shot up as soya bean seedling; Generally see light processing 3-4 days, the time, too short bean sprouts can not grown, and available bean seedlings blade very little; Overlong time, too high will the lodging that bean seedlings are long, and grow to the phenomenon that to a certain degree there will be following blade flavescence, wilts, comes off, thus affect taking food of Frankliniella occidentalis.The soya bean seedling shifting out will be refreshed the water periodically, and general 2 days once.
After moyashi and soya bean seedling are cultivated, with it, raise Frankliniella occidentalis.First the moyashi with oviposition substrate is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, and blotting paper of cylinder bottom pad keeps moistening, and incubation condition is 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D.When having nymph to occur, to rest in the box for feeding pests that worm cylinder is transferred to the fresh bean seedlings of whole strain, nymph can go to voluntarily on soya bean seedling and take food.The cultivation of soya bean seedling will note having enough moisture to grow by it, but water level will not have the root system water level of bean sprouts must be lower than the pallet 2cm that holds soya bean, in case Frankliniella occidentalis is overboard drowned while taking food.When bean seedlings approach wilting, place the soya bean seedling that a dish is new, Taeniothrips spp shifts out the bean seedlings of wilting after shifting.General one bean seedlings that put one's child in a boarding nursery dish can take food 4-6 days for 50 thrips.
Adopting indoor water planting bean seedlings method to feed, is the main path that solves seasonal feed shortage.Although slightly variant to the demand of indivedual nutriments between different insects kind, the nutritional need of nearly all insect is substantially similar, comprises protein, carbohydrate, lipid, vitamin, the former material of fat, mineral salt and moisture etc.In the moyashi that the present invention adopts, except contain the vitamin C generating in germination process, also contain the abundant mineral matters such as quality plant protein contained in original soya bean and vitamin B1, vitamin B2, calcium, potassium, phosphorus and iron.Under the partial starch of storing at Interior Seed during soybean germination and the effect of protein at enzyme, decompose, change into the needed carbohydrate of growth and amino acid, starch and protein utilization in soya bean are improved greatly; In addition, the present invention adopts moyashi and the soya bean seedling oneself cultivated to realize the autonomous cultivation to Frankliniella occidentalis, thereby avoids the experiment of some pesticide resistance detections and medicament resistance screening to impact.
In sum, the employing water planting bean seedlings of the invention are raised Frankliniella occidentalis, employing is shown in that the soya bean seedling that light is processed 3-4 days carries out feeding of Frankliniella occidentalis nymph and adult, employing has no the moyashi of light processing as oviposition substrate, not only utilized bean sprouts but also utilized bean seedlings, the nutritional need that grows and breed that can meet preferably Frankliniella occidentalis, has reduced again feeding cost.The impact that the requirement of the production environment of water planting bean seedlings is low in addition, production cost is low, growth cycle is short, grow and be not subject to seasonal effect, be not subject to external environment, for the extensive Frankliniella occidentalis of raising provides the basis on feed.
Embodiment
Embodiment 1
A raising method for Frankliniella occidentalis, its concrete steps are:
(1) water planting of soya bean seedling: select full seed, without insect population, the soya bean that goes mouldy, be placed on the pallet that has osculum in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading cultivation, in germination process, in time rotten beans, the soya bean that do not germinate are not in time chosen, in case mouldy and cause that other bean seedlings rot.When bean sprouts is germinateed to 2cm, to adding paclobutrazol solution to the concentration of paclobutrazol in whole water planting liquid in water planting liquid, be 10ppm, when growing to 12cm, bean sprouts shifts out bean sprout growing machine, and carry out fluorescent lamp and see that light processes 3 days, grow into soya bean seedling;
(2) raising of Frankliniella occidentalis: the moyashi with Frankliniella occidentalis ovum is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, and blotting paper of cylinder bottom pad keeps moistening, and incubation condition is 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D.When ovum hatching has nymph to occur, rest worm cylinder and be transferred in the box for feeding pests that is placed with the fresh bean seedlings of whole strain, box for feeding pests is placed in insectary, in relative moisture, is 75% ± 5, temperature is 25 ± 2 ℃, under the condition of illumination 16L:8D, Frankliniella occidentalis nymph and adult is fed.
Embodiment 2
A raising method for Frankliniella occidentalis, its concrete steps are:
(1) water planting of soya bean seedling: select full seed, without insect population, the soya bean that goes mouldy, be placed on the pallet that has osculum in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading cultivation, in germination process, in time rotten beans, the soya bean that do not germinate are not in time chosen, in case mouldy and cause that other bean seedlings rot.When bean sprouts is germinateed to 3cm, to adding paclobutrazol solution to the concentration of paclobutrazol in whole water planting liquid in water planting liquid, be 20ppm, when growing to 9cm, bean sprouts shifts out bean sprout growing machine, and carry out fluorescent lamp and see that light processes 4 days, grow into soya bean seedling;
(2) raising of Frankliniella occidentalis: the moyashi with Frankliniella occidentalis ovum is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, and blotting paper of cylinder bottom pad keeps moistening, and incubation condition is 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D.When ovum hatching has nymph to occur, rest worm cylinder and be transferred in the box for feeding pests that is placed with the fresh bean seedlings of whole strain, box for feeding pests is placed in insectary, in relative moisture, is 75% ± 5, temperature is 25 ± 2 ℃, under the condition of illumination 16L:8D, Frankliniella occidentalis nymph and adult is fed.
Embodiment 3
A raising method for Frankliniella occidentalis, its concrete steps are:
(1) water planting of soya bean seedling: select full seed, without insect population, the soya bean that goes mouldy, be placed on the pallet that has osculum in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading cultivation, in germination process, in time rotten beans, the soya bean that do not germinate are not in time chosen, in case mouldy and cause that other bean seedlings rot.When bean sprouts is germinateed to 4cm, to adding paclobutrazol solution to the concentration of paclobutrazol in whole water planting liquid in water planting liquid, be 15ppm, when growing to 10cm, bean sprouts shifts out bean sprout growing machine, and carry out fluorescent lamp and see that light processes 4 days, grow into soya bean seedling;
(2) raising of Frankliniella occidentalis: the moyashi with Frankliniella occidentalis ovum is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, and blotting paper of cylinder bottom pad keeps moistening, and incubation condition is 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D.When ovum hatching has nymph to occur, rest worm cylinder and be transferred in the box for feeding pests that is placed with the fresh bean seedlings of whole strain, box for feeding pests is placed in insectary, in relative moisture, is 75% ± 5, temperature is 25 ± 2 ℃, under the condition of illumination 16L:8D, Frankliniella occidentalis nymph and adult is fed.
Comparative example
It is example that the kidney bean of take is raised Frankliniella occidentalis.
The kidney bean that market is bought, soaks and removes as far as possible remains of pesticide in 2-3 hour with clear water, with blotting paper, sucks excessive moisture, prevents from occurring rotting in the process of feeding.Under 25 ± 2 ℃, RH=75 ± 5%, 16L:8D illumination condition, Frankliniella occidentalis (100-200 head) and fresh kidney bean are placed in to side opening to be supported worm cylinder and raises, cylinder mouth is tamping with gauze, blotting paper of cylinder bottom pad keeps moistening, after wilting, kidney bean changes feed, and approximately 5-7 days can be for raising.The general generation of breeding for 25-30 days.
By indoor fresh kidney bean and the continuous single head of two kinds of feeds of water planting bean seedlings, raise Frankliniella occidentalis, find that fresh kidney bean is suitable with water planting bean seedlings raising Frankliniella occidentalis effect, the results are shown in Table 1.
Table 1 embodiment 1-3 and the contrast of comparative example result
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example | |
| Nymphal development is gone through the phase | 4.79 my god | 4.77 my god | 4.81 my god | 4.79 my god |
| Lethality | 18％ | 19％ | 17％ | 13％ |
| Egg laying amount | 54 | 50 | 58 | 63 |
| Life of Adult | 18 days | 20 days | 19 days | 20 days |
As seen from Table 1:
(1) the Frankliniella occidentalis nymph average mortality that the present invention raises is 18%, a little more than the Frankliniella occidentalis 13% of feeding with kidney bean;
(2) the average development duration of Frankliniella occidentalis nymph that the present invention raises is 4.79d, suitable with kidney bean feeding effect, for the adult stage accumulates enough nutriments, has extended life-span and the egg-laying period of adult.
(3) the Frankliniella occidentalis adult mean lifetime that the present invention raises is 18d left and right, and the Frankliniella occidentalis Life of Adult that fresh kidney bean is fed reached about 20 days.
(4) when the Frankliniella occidentalis Adult worms producting eggs amount of the present invention's raising is maximum, can reach 58, egg production peak time concentrates on the 4th to 9 days, and the average egg-laying period is 14 days.The Frankliniella occidentalis Adult worms producting eggs amount that fresh kidney bean is fed is 63, and egg production peak time also concentrates on the 4th to 9 days, and the average egg-laying period is 14 days.
By above contrast, find, the present invention adopts the feeding effect of water planting bean seedlings to compare with now conventional desirable plants source feed-kidney bean, nymphal development is gone through the phase, lethality, egg laying amount, Life of Adult are very close, but cost reduces greatly, the impact that production environment requirement is low, growth cycle is short, grow and be not subject to seasonal effect, be not subject to external environment, the impact of effectively having avoided feed medication to cause result of the test.
Claims (5)
1. a raising method for Frankliniella occidentalis, is characterized in that: adopt water planting bean seedlings to raise Frankliniella occidentalis;
Employing is shown in that the soya bean seedling that light is processed 3-4 days carries out feeding of Frankliniella occidentalis nymph and adult, adopts the new moyashi that has no light processing as oviposition substrate;
Its concrete steps are:
(1) water planting of soya bean seedling: select full seed, without insect population, the soya bean that goes mouldy, be placed in bean sprout growing machine, in 25 ± 2 ℃, add water moisturizing, shading cultivation, when bean sprouts is germinateed to 2-4cm, in water planting liquid, add paclobutrazol solution, when bean sprouts grows to 9-12cm, shift out bean sprout growing machine, carry out fluorescent lamp and see light processing, grow into soya bean seedling;
(2) raising of Frankliniella occidentalis: the moyashi with Frankliniella occidentalis ovum is placed in to foster worm cylinder and hatches, cylinder mouth is tamping with gauze, when ovum hatching has nymph to occur, resting worm cylinder is transferred in the box for feeding pests that is placed with the fresh bean seedlings of whole strain, box for feeding pests is placed in insectary, and Frankliniella occidentalis nymph and adult are fed;
Described moyashi is in step (1), to see that it is 2-4cm moyashi that light is processed previous length.
2. the raising method of Frankliniella occidentalis according to claim 1, is characterized in that: in step (1), adding after paclobutrazol solution the concentration of paclobutrazol in whole water planting liquid is 10-20ppm.
3. the raising method of Frankliniella occidentalis according to claim 1, is characterized in that: in step (1), see that the light processing time is 3-4 days.
4. the raising method of Frankliniella occidentalis according to claim 1, is characterized in that: the nymph incubation condition in step (2) is 25 ± 2 ℃ of temperature, relative moisture 75 ± 5%, illumination 16L:8D.
5. the raising method of Frankliniella occidentalis according to claim 1, is characterized in that: in step (2), in insectary, relative moisture is 75% ± 5, and temperature is 25 ± 2 ℃, illumination 16L:8D.
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| CN102640729B true CN102640729B (en) | 2014-02-26 |
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| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
| CN105875507B (en) * | 2014-05-14 | 2018-08-28 | 中国农业科学院植物保护研究所 | A kind of method of artificial mass production pest natural enemy |
| CN104782574B (en) * | 2014-12-09 | 2017-04-26 | 青岛农业大学 | Frankliniella occidentalis arrhenotoky inbreeding population establishing method |
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| CN105660546B (en) * | 2016-04-01 | 2018-02-06 | 云南省农业科学院花卉研究所 | A kind of Frankliniella occidentalis indoor feeding method |
| CN108684614B (en) * | 2018-04-27 | 2023-12-12 | 云南农业大学 | Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method |
| CN108391628B (en) * | 2018-04-27 | 2023-08-22 | 云南农业大学 | Feeding device for frankliniella occidentalis |
| CN109042537A (en) * | 2018-08-03 | 2018-12-21 | 福建省农业科学院植物保护研究所 | A kind of method for breeding of palm thrips |
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| CN112931417A (en) * | 2021-02-26 | 2021-06-11 | 中国农业科学院植物保护研究所 | Method for group breeding of frankliniella occidentalis and application thereof |
| CN112931427B (en) * | 2021-04-28 | 2022-05-17 | 内蒙古农业大学 | Indoor breeding method for frankliniella occidentalis suitable for northern area |
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