CN106689069A - Large-scale feeding and storage method of aphids and predators of aphids - Google Patents
Large-scale feeding and storage method of aphids and predators of aphids Download PDFInfo
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- CN106689069A CN106689069A CN201710047490.4A CN201710047490A CN106689069A CN 106689069 A CN106689069 A CN 106689069A CN 201710047490 A CN201710047490 A CN 201710047490A CN 106689069 A CN106689069 A CN 106689069A
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- 241001124076 Aphididae Species 0.000 title claims abstract description 93
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000003860 storage Methods 0.000 title claims abstract description 36
- 244000062645 predators Species 0.000 title claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 45
- 241000952610 Aphis glycines Species 0.000 claims abstract description 41
- 241000255749 Coccinellidae Species 0.000 claims abstract description 40
- 244000068988 Glycine max Species 0.000 claims abstract description 12
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 11
- 241000382353 Pupa Species 0.000 claims abstract description 6
- 238000009395 breeding Methods 0.000 claims description 38
- 230000001488 breeding effect Effects 0.000 claims description 38
- 102000002322 Egg Proteins Human genes 0.000 claims description 16
- 108010000912 Egg Proteins Proteins 0.000 claims description 16
- 210000004681 ovum Anatomy 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 8
- 230000012447 hatching Effects 0.000 claims description 7
- 230000000474 nursing effect Effects 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 4
- 241001582888 Lobus Species 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 16
- 230000009286 beneficial effect Effects 0.000 abstract description 2
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 16
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- 239000010455 vermiculite Substances 0.000 description 9
- 230000004083 survival effect Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 5
- 239000000123 paper Substances 0.000 description 5
- 241001292007 Chrysopa Species 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001600407 Aphis <genus> Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 2
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- 241000233866 Fungi Species 0.000 description 2
- 239000004836 Glue Stick Substances 0.000 description 2
- 241000199695 Harmonia <beetle> Species 0.000 description 2
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- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 241001064674 Chrysopa septempunctata Species 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 1
- 229940056881 imidacloprid Drugs 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a large-scale feeding and storage method of aphids and predators of the aphids. The method comprises the following steps: Step 1, feeding soybean aphids by taking soybeans as host plants, wherein the soybean aphids are used for feeding ladybirds; Step 2, incubating ova of the ladybirds into larvas in concentration manner and feeding the soybean aphids with the larvas so that the soybean aphids pupate; Step 3, performing eclosion on pupas formed in Step 2, so as to obtain adults; Step 4, feeding the adults in Step 3 in pairs, so as to obtain ova of the ladybirds, and placing the ova of the ladybirds into a cold cupboard for dark treatment; and Step 5, storing the adults in Step 3 with the soybean aphids. The method has the beneficial effects that large-scale, low-cost and standardized production of the aphids and the predators of the aphids, so that the large-scale production of high-quality coccinelidae and lacewing comes true, and the commercialization of the ladybirds and the lacewing is promoted.
Description
Technical field
The present invention relates to a kind of aphid and its predator large-scale breeding, storage method, more specifically to aphid
The culture of worm, the concentration hatching of Ladybird egg, larva raising, mature larva concentrate pupate, the pair fed of adult, the receipts of commodity ovum
Collection and the adjustment of storage and the storage of commodity grandis adults.
Background technology
Aphid is the important pests for endangering multiple kinds of crops growth, with extremely strong fertility and destructiveness, Chang Zhi
Food water is sucked in the positions such as thing tender leaf, tender stem, bud, plant Tender points by thorn, causes plant nutrition to be lost in and damage
Plant organ.Additionally, aphid can largely secrete honeydew in breeding, harm, these honeydews are the breeding of some fungies and bacterium
There is provided splendid nutriment so that fungi and the rapid breeding dispersal of bacterium energy, substantial amounts of mould dirt is formed, cause plant disease
Generation with it is popular.
The preventing and treating of aphid is entered by the chemical agent based on the insecticide of absorbability such as imidacloprid always for a long time
OK, these medicaments serve important function in terms of aphid damage is controlled, but due to long-term use, aphid is to such medicament
The resistance to the action of a drug it is more and more stronger, cause the matched proportion density more and more higher of these medicaments, cause increasing food security sex chromosome mosaicism.
The hostile various aphids of food aphid day based on ladybug, Chrysopa have good control action, and multiple worm states of these natural enemies can
Predation aphid, and predatory number is big, it is long to prey on the cycle, and irreplaceable important function is played in agriculture and forestry production.And with
Green, the development of organic agriculture, it is more and more using limitation to chemical insecticide, to the food aphid day based on ladybug, Chrysopa
The demand of enemy is more and more vigorous.
In recent years, about ladybug man-made feeds and the research about aspects such as ecology, the characteristics of biology are achieved
Incremental advances.But its scale and standardization breeding technology it is also to be improved, breeding facility, produce in enormous quantities technological standards,
The aspects such as packing and storing technology all there are problems that larger, therefore limit that outstanding natural enemy insect is in good time, substantial amounts of supply, it is impossible to
The need for meeting production at present.
And the numerous bottleneck for being to eat aphid natural enemy large-scale production of extensive, inexpensive expansion of aphid, particularly in the northern this winter
My god, summer long-term area, it is however generally that the good external condition of the raising need of aphid supports, such as winter when need
Heating equipment, summer needs the cooling installations such as air-conditioning, and in general aphid expands numerous needs larger as the food source of food aphid natural enemy
Space support, virtually increased the reproductive-cost of its predator.
The subject matter of production aphid presence is at this stage：
(1) suitable host plant is not chosen.At present, the host plant of breeding aphid is typically Cruciferae plant
Thing, such as Chinese cabbage, radish, rape.Although these plants can breed a number of aphid, exist the breeding cycle it is long, produce
Amount is low, require there is that greenhouse, collection are difficult；Other these host plants are difficult to realize standardized cultivation, thus also
The standardization that aphid is raised cannot be realized.
(2) without suitable propagation method.Existing aphid propagation method is only when host plant grows into certain rank
Aphid is accessed during section, it is impossible to make full use of the nutrition of host plant, while being disturbed larger by Host plant resistance, work as host plant
During robust growth, aphis population slower development, or even occur declining phenomenon.Natural enemy insect is difficult control in other production environment,
When there is natural enemy insect to be difficult to remove when invading breeding environment, aphis population is caused to be compressed on a relatively low level all the time.
(3) without the technology of a large amount of, quick batch production aphids.Existing aphid propagation method is past to worm is connect from seedling
It is past to need 30-40 days, thus the consumption energy is more, many using manpower, the greenhouse space of occupancy is also than larger, it is impossible to carry out concentration product
Go out and standardized production.
(4) seasonal dependence is strong.At present, breeding aphid can only carry out artificial propagation, one in the season for being adapted to aphid growth
As be 2-3 month of spring and autumn time in, other times are difficult to ensure because of humiture, or the more difficult acquisition of host plant,
Thus can not largely be produced.
The content of the invention
Instant invention overcomes deficiency of the prior art, there is provided a kind of aphid and its predator large-scale breeding,
Storage method, realize that integrated cost is low, breeding amount big, numerous natural enemy quality better scale operation system, it is intended to reduce me
Numerous cost is expanded in state, the scale of particularly northern area food aphid natural enemy, realizes low cost, the high-quality production of food aphid natural enemy.This
The purpose of invention is achieved by following technical proposals.
A kind of aphid and its predator large-scale breeding, storage method, the method are comprised the following steps：
The first step, soybean aphid is raised by the use of soybean as host plant, and soybean aphid is used to raise ladybug；
Second step, concentrates hatching to larva Ladybird egg, carries out raising to larva with soybean aphid and pupates；
3rd step, the pupa formed in selection second step is sprouted wings, and obtains adult；
4th step, pairing raising is carried out to the adult in third step, obtains Ladybird egg, and Ladybird egg is placed in refrigerator
Carry out dark treatment；
5th step, is stored using soybean aphid to the adult in third step, and storing step is as follows：
1) first time nursing is carried out to adult using soybean aphid, raises number of days 15-20 days, adult is entered in refrigerator
Row dark treatment；
2) using soybean aphid to step 1) in the adult of dark treatment carry out second nursing, raise number of days 1-2 days, will hello
Adult after supporting continues to carry out dark treatment in refrigerator；
3) repeat step 2) feed, until raising to required storage number of days.
Further, in the first step, with host plant raise soybean aphid the step of it is as follows：1) culture host plants
Thing, after host plant emerges 7-9 days, when two panels cotyledon launches, carries out first time inoculation, and inoculum concentration is every young plant 5-7 heads
Aphid；2) by after 3-4 days, when the first piece lobus cardiacus of host plant seedling launches, carrying out second inoculation, inoculum concentration is every plant
Seedling 10-15 aphid；3) it was a batch per 3-5 days, repeats 1) step, 2) step.
Further, host plant culture, the condition of host plant raising soybean aphid are：Temperature is 15-35 DEG C, relatively wet
It is 40%-80% to spend, and light application time is 8-16 hours.
Further, host plant culture, the condition of host plant raising soybean aphid are：Temperature is 15-35 DEG C, relatively wet
It is 40%-80% to spend, and with the fluorescent lamp of 40W as light source, fluorescent lamp is positioned at the 30-40cm of bean seedlings top.
Further, temperature control, at 20-26 DEG C, is pupated to larva raising and is divided into four-stage in the second step：The
The raising ratio of one stage, raising first-instar young to second instar larvae, first-instar young and soybean aphid is 1:8-1:15；Second-order
The raising ratio of section, raising second instar larvae to third-instar larvae, second instar larvae and soybean aphid is 1:15-1:25；Phase III, raise
Third-instar larvae is supported to four-age larva, third-instar larvae is 1 with the raising ratio of soybean aphid:30-1:40；Fourth stage, raises four
To pupating, the raising ratio of four-age larva and soybean aphid is 1 to instar larvae:50-1:60.
Further, sex ration 1 is pressed in the four steps:1 carries out adult pairing raises, and collects a deutovum within every 12 hours,
Ovum is concentrated to be placed on dark treatment is carried out in refrigerator, the temperature setting of refrigerator is 8-10 DEG C, the condition that adult pairing is raised is：
Light irradiation time is 14-16 hours, temperature is 20-26 DEG C, and humidity is 50%-80%.
Further, the condition fed for the first time in the 5th step is：The raising ratio of ladybug and aphid is 1:100,
Feeding environment temperature is 13-20 DEG C, and the photoperiod is (6~10) L：(14~18) D, relative humidity is 60%-80%.
Further, the condition of second nursing is in the 5th step：In 5th step second feed article
Part is：The raising ratio of ladybug and aphid is 1:100, feeding environment temperature is 13-20 DEG C, and the photoperiod is (6~10) L：(14~
18) D, relative humidity is 60%-80%.
Further, it is 35-45 days to be fed each time in the 5th step with the last time interval fed.
Further, the temperature of refrigerator is 4-6 DEG C in the 5th step.
Beneficial effects of the present invention are：From No. 12 host plants as breeding soybean aphid of Jidou 10 or Ji beans, have
Grown soon after price is low, germination percentage is high, and aphid takes food, substantially reduce the breeding cycle, increased the yield of aphid, realize aphid
The standardization that worm is raised；
Aphid breeding is carried out under natural Thermo-Photo sensitive, is shone as light source using fluorescent lamp in the case of natural light deficiency
It is bright, save cost and substantial amounts of power cost that greenhouse is used；Vermiculite can be reused, and reduce the cost for cultivating aphid；
It by becoming be once multiple that aphid is inoculated with, and makes full use of nutrition and the speed of growth of host plant, 7-10 days when
Between in more than 50 times of the aphid output increased of unit leaf area can be convenient for concentrating output；It is disturbed factor ratio simultaneously
It is less, being capable of energy saving and manpower；
There is no seasonal dependence, whole year production can be carried out, in disclosure satisfy that 1 year the need for multiple months, make full use of
Production facility, saves equipment cost；
It is the important predation of various food aphid natural enemies such as harmonia axyridia, Propylaea japonica, chrysopa septempunctata, chrysopa carnea from soybean aphid
Object, is the existence in food aphid natural enemy external environment out of office and breeding particularly under the hot summer environmental condition of northern area
Originated there is provided important foodstuff, there is fast growth, environment to adapt to the various features such as strong for it, in 35 DEG C of high temperature, moderately low
Can quickly be bred under conditions of 12 DEG C of temperature, be that itself and predator vast propagation are provided convenience condition；
Commodity ovum and adult collection method are simple and quick, substantial amounts of ovum and adult can be harvested in the short time, and need not hinder
And ovum and adult, also allow for number of computations and further apply, realize the standardization of ladybug production；
Commodity ovum can be stored 15-20 days, commodity adult 150-200 days is conducive to effectively adjusting the fluctuation of output,
More preferable order of arranging production.
The aphid artificial fecundation method that the present invention is provided can realize large-scale breeding, low cost and the standardization of aphid, be
The growth and breeding of world insect constantly provides the feed of abundance, using this technology, is capable of achieving the predatism such as red shoulder ladybug day
Enemy's storage survival rate is improved, and for the biological control of aphid class pest provides natural enemy product, enriches biological control means, is improve
The science of agricultural pests administers level, realizes extensive, the inexpensive and standardization of aphid, natural enemy production so that extensive
The high-quality food aphid ladybug of production, Chrysopa become a reality, and promote the commercialization of ladybug, Chrysopa.
Brief description of the drawings
Fig. 1 is aphid of the present invention and its predator large-scale breeding, the flow chart of storage method.
Specific embodiment
It is lower of the invention to reach technological means and effect that predetermined goal of the invention is taken further to illustrate, below tie
Preferred embodiment is closed, structure of the invention, workflow is described in detail as follows：
A kind of aphid and its predator large-scale breeding, storage method, the method are comprised the following steps：
The first step, soybean aphid is raised by the use of soybean as host plant, and soybean aphid is used to raise ladybug, and method is such as
Under:
1st, the cultivation of host plant
From No. 12 host plants as breeding soybean aphid of Jidou 10 or Ji beans, will be any in above two soybean
A kind of soybean carries out aphid and raises on a large scale as the host plant that aphid is cultivated, and specific method is as follows：
It is (length) 32 × 24 × 10cm, bottom even 24 apertures (aperture 0.8cm-1cm) of distribution to specification
Load new or vermiculite by sterilization treatment in plastic casing until plastic casing 5cm eminences, are fitted filled out vermiculite with hand or instrument
Work as concora crush, make its filling slightly consolidation, then by above-mentioned Soybean Species uniform broadcasting to vermiculite upper strata, 250-300 is about broadcasted sowing per box.
After the completion of sowing, in the above-mentioned vermiculite for having sowed an evenly laid out thickness about 1cm above soybean.Then one group of 2 box, is positioned over rule
Lattice are in the plastic pallet of (length) 60 × 35 × 5cm, and the lower floor that plastic pallet horizontal vertical is placed in into foster worm frame is (long
3m, 2.5m high, 50cm wide, 4 layers).It it is 15-35 DEG C in temperature, relative humidity is 40%-80%, and natural light is used as light source, illumination
Time is to cultivate host plant under conditions of 8-16 hours, slowly, is repeatedly added water in plastic pallet in incubation, until band
Untill plastic casing vermiculite at the middle and upper levels in hole is slightly wet.
When natural light is insufficient as light source, with the fluorescent lamp of 40W light source as an alternative, fluorescent lamp is positioned over distance
At the 30-40cm of bean seedlings top.
2nd, in the Seedling Inoculation aphid of soybean, aphid is bred in culture, and method is as follows：
Under conditions of host plant is cultivated, after 3-5 days, soybean emerges, and after 7-9 days, big bean seedlings two panels cotyledon launches, will
Pallet moves to 3-4 layers of foster worm frame, now carries out first time inoculation, and inoculum concentration is every young plant 5-7 aphid；After about 11 days, work as seedling
When first piece lobus cardiacus launches, second inoculation is carried out, inoculum concentration is every young plant 10-15 aphid；15 days or so, aphid was just covered with
Whole soybean young plant night upper portion, the now every plant big aphid amount of bean seedlings about 200, each plastic casing about 50000-60000
Head aphid.
During soybean bean seedling growth is developed, temperature, the humidity condition of wetness conditions and natural conditions according to vermiculite are mended in time
Water-filling point, in case big bean seedlings water shortage, influences normal growth.
3rd, the batch production of aphid
It was a batch per 3-5 days, repeats aforesaid operations, i.e.,：After sowing for the first time 3 days, proceed by second
Sowing, operates when subsequent operation is with above-mentioned inoculation.Subsequent batches are carried out like this.After aphid is using finishing, by plastic casing
Vermiculite is removed in the lump together with bean seedlings, and vermiculite is recycled, removal of impurities, dry, sterilize after by reuse.
Second step, concentrates hatching to larva Ladybird egg, carries out raising to larva with soybean aphid and pupates
The ladybug of large-scaled propugation production can select red shoulder ladybug, Propylaea japonica, harmonia axyridia, coccinella septempunctata, polyisocyanate wooden dipper
The Predaceous Coccinellids such as worm, in the present embodiment by taking red shoulder ladybug as an example, illustrate to ladybug large-scale breeding, storage method, side
Method is as follows：
1st, the concentration hatching of Ladybird egg
The ovum that collection grandis adults are produced in 24 hours is in upper bottom diameter 7cm, the transparent modeling of go to the bottom diameter 5cm, 10cm high
In material cup, and with the upper bottom of gauze closure, hatching treatment is carried out under the conditions of being placed in 20-26 DEG C, ovum is a large amount of hatchings after general 2-3 days
Into 1 instar larvae, the larva transfer that will now hatch, the concentration for starting larva is raised.
2nd, the raising of larva, method for breeding is as follows：
1) one, second instar larvae is positioned in the plastic casing that specification is (length) 24 × 36 × 8cm and is raised, raise box
Interior to place the cardboard that 1 20cm long, width 10cm prolong width folding repeatedly, plastic casing top is blocked with 80 mesh gauzes, with weldering
Rifle melts glue stick, glue stick is uniformly applied to plastic casing top surrounding after melting, and only stays 10cm long, the osculum of width 6cm, is easy to
Aphid and taking-up are delivered, under conditions of being 20-26 DEG C in feeding environment temperature, the raising ratio of first-instar young and soybean aphid is
1:8-1:15, the raising ratio of second instar larvae and soybean aphid is 1:15-1:25.
During feeding larva, 17 days big bean seedlings of left and right belt aphid are cut, be thrown in above-mentioned plastic casing, change once daily
Aphid.When changing aphid, ladybug developmental state is observed, aphid supply is increased according to ladybug different developmental phases.
2) three, four-age larva is positioned in the sarong that specification is (length) 80 × 80 × 80cm and is raised,
Feeding environment temperature is under conditions of 20-26 DEG C, the raising ratio of third-instar larvae and soybean aphid is 1:30-1:40, four ages children
The raising ratio of worm and soybean aphid is 1:50-1:60.
The seedling age 17 day big bean seedlings of left and right belt aphid of the box by the breeding flow production of above-mentioned aphid are placed in sarong, in box
The suitable plastic pallet of specification is placed in lower section, in order to supplement big bean seedlings moisture.When the aphid number on big bean seedlings in sarong is not enough
When, the big bean seedlings that above-mentioned seedling age is 17 days or so are supplemented in time.
3rd step, the pupa formed in selection second step is sprouted wings, and obtains adult, and method is as follows：
By the supreme bottom diameter 7cm of first group of collection of mature larva 40-50, bottom diameter 5cm, 10cm high transparent plastic cup
In, it is placed with the folding hard paper of interfolded in cup, the 10-15 big bean seedlings of band aphid for shearing are placed on scraps of paper top, and by plastics
Cup upper shed is raised with the upper bottom of gauze closure under conditions of being 20-26 DEG C in feeding environment temperature.
Mature larva body is in canescence, and due to that will pupate, few food or stopping take food, slow in action, mature larva
Prepupal period general 1 day or so, i.e., mature larva is just largely pupated in scraps of paper burst after 1 day.Ladybug pupa just starts plumage in general 3 days
Change, be within the 4th day the peak period of pupa emergence, the adult of emergence can typically assemble on the gauze of upper sealing part.
4th step, 1, carry out pairing raising to the adult in third step, Ladybird egg is obtained, method is as follows：
Female adult pest and male imago are carried out 1:1 pairing, raise in upper bottom diameter 7cm, bottom diameter 5cm, 10cm high it is saturating
Big bean seedlings of band aphid that a folding white hard paper piece and 3 shear are placed with bright plastic cup, in cup, and (enough 2 adults 2 days take food
Amount), and be 20-26 DEG C, humidity 50%-80%, illumination in feeding environment temperature by plastic cup upper shed with the upper bottom of gauze closure
Raised under the rearing conditions of duration 14-16 hours.
Adult starts spawning for 7 days or so, and ovum is most to be produced on white folded sheet, in addition to spawning in first 3-4 days is less
(general 20-30), all at 80 or so, part is even as high as 140 to follow-up continuous 20 days layings.
2nd, commodity ovum is collected and is stored, method is as follows：
After adult starts spawning, according to production and order arrangement, the ovum grain that will temporarily be not used as breeding is refrigerated, stored
Treatment.Specific method is as follows：Collect a deutovum within every 12 hours, it is straight that the white folded sheet that above-mentioned product has ovum grain is collected into supreme bottom
In footpath 7cm, the diameter 5cm that goes to the bottom, the transparent plastic cup of 10cm high, about 1000 one group is placed in same plastic cup, then writes
Collect the date, concentration carries out awkward silence at a meeting treatment in being placed on refrigerator in dark place, and the temperature of refrigerator is 8-10 DEG C.
The storage condition of the commodity ovum of table 1 is explored
From experiment, the storage time of commodity ovum should be no more than 10 days, and now survival rate highest, stores number of days more than 10
It when survival rate dramatic decrease.When the storage number of days of commodity ovum was not used still more than 10 days, should in time take out, can do follow-up
The basic worm sources of breeding, also or are discharged into big Tanaka.
5th step, is stored using soybean aphid to the adult in third step, and method is as follows：
Just sprout wings in selection third step, stored not as stock adult, 40 first groups of concentrations are raised in rule
Lattice are raised placement 1 20cm long, width 10cm in box and prolong width repeatedly to be raised in the plastic casing of (length) 36 × 24 × 8cm
The cardboard that degree direction folds, 1 is compared by ladybug, aphid:100 ratio is raised, and feeding environment temperature is 15 DEG C, and the photoperiod sets
It is set to L：D=8:16, relative humidity is set to 70%, is raised 20 days or so under the conditions of this, and the ladybug 40 of above-mentioned raising is first
Group is collected in the transparent plastic cup of supreme bottom diameter 7cm, the diameter 5cm that goes to the bottom, 10cm high, and the folding that interfolded is placed with cup is hard
The scraps of paper, awkward silence at a meeting treatment, the temperature of refrigerator are carried out by plastic cup upper shed to be placed in the cabinet of Tibetan behind the upper bottom of gauze closure in dark place
It is set to 4-6 DEG C.Hereafter 1 is compared by ladybug, aphid under the conditions of former imago breeding within every 40 days:100 ratio raising 1 day, then
Continue to be held in former refrigerator.
The storage suitability of the commodity adult of table 1
Grandis adults memory is stored using above method, grandis adults are stored 80 days, and survival rate is still maintained at 98%
Left and right；Storage 160 days, survival rate is still maintained at 80% or so；200 days survival rates of storage are still maintained at 70% or so；Storage
Deposit 240 days, survival rate is still maintained at more than 50%.
For the commodity grandis adults for storing different time length, the basic number of required adult when being discharged for the clear and definite later stage
The biological indicator situation of ladybug after amount and storage different time, be in temperature to the female grandis adults after storage different number of days
24 ± 1 DEG C, the photoperiod be L：D=16:8th, humidity is to be raised under conditions of 70%, evaluates its egg-laying season, life-span, spawning total
Amount three three indexs closely related with follow-up prevention and control ability (using average value ± SE).
Table 2 stores the biological indicator evaluation of adult
Result shows：Storage is had no significant effect for 40 days to adult principal biological index, and adult basic is raised with normal
Cause；Storage time is more long, and female adult egg-laying season, life-span, spawning total amount are all in obvious downward trend, and when storing 160 days, items refer to
Mark drops to normal half for raising adult or so；When storing 240 days, adult indices drop to the 1/ of normal raising adult
6 or so.In terms of biological evaluation result from after storage, adult storage time is most long no more than 160 days.
One embodiment of the present of invention has been described in detail above, but the content is only preferable implementation of the invention
Example, it is impossible to be considered as limiting practical range of the invention.All impartial changes made according to the present patent application scope and improvement
Deng all should still belong within patent covering scope of the invention.
Claims (9)
1. a kind of aphid and its predator large-scale breeding, storage method, it is characterised in that：The method includes following step
Suddenly：
The first step, soybean aphid is raised by the use of soybean as host plant, and soybean aphid is used to raise ladybug；
Second step, concentrates hatching to larva Ladybird egg, carries out raising to larva with soybean aphid and pupates；
3rd step, the pupa formed in selection second step is sprouted wings, and obtains adult；
4th step, pairing raising is carried out to the adult in third step, obtains Ladybird egg, and Ladybird egg is placed in refrigerator to be carried out
Dark treatment；
5th step, is stored using soybean aphid to the adult in third step, and storing step is as follows：
1) first time nursing is carried out to adult using soybean aphid, raises number of days 15-20 days, adult is carried out secretly in refrigerator
Treatment；
2) using soybean aphid to step 1) in the adult of dark treatment carry out second nursing, number of days is raised 1-2 days, after nursing
Adult continue dark treatment is carried out in refrigerator；
3) repeat step 2) feed, until raising to required storage number of days.
2. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
It is as follows the step of raise soybean aphid with host plant in stating first step：1) host plant is cultivated, is emerged 7- in host plant
After 9 days, when two panels cotyledon launches, first time inoculation is carried out, inoculum concentration is every young plant 5-7 aphid；2) by after 3-4 days,
When the first piece lobus cardiacus of host plant seedling launches, second inoculation is carried out, inoculum concentration is every young plant 10-15 aphid；3) it is every
3-5 days is a batch, repeats 1) step, 2) step.
3. aphid according to claim 2 and its predator large-scale breeding, storage method, it is characterised in that：Post
Main plant culture, the condition of host plant raising soybean aphid are：Temperature is 15-35 DEG C, and relative humidity is 40%-80%, light
It it is 8-16 hours according to the time.
4. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
Temperature control is pupated to larva raising and is divided into four-stage at 20-26 DEG C in stating second step：First stage, raise age children
To second instar larvae, the raising ratio of first-instar young and soybean aphid is 1 to worm:8-1:15；Second stage, raises second instar larvae to three
The raising ratio of instar larvae, second instar larvae and soybean aphid is 1:15-1:25；Phase III, raise third-instar larvae to four ages children
The raising ratio of worm, third-instar larvae and soybean aphid is 1:30-1:40；Fourth stage, raises four-age larva to pupating, and four ages are young
The raising ratio of worm and soybean aphid is 1:50-1:60.
5. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
State in four steps by sex ration 1:1 carries out adult pairing raises, and collects a deutovum within every 12 hours, ovum is concentrated and is placed on refrigerator
In carry out dark treatment, the temperature setting of refrigerator is 8-10 DEG C, and the condition that adult pairing is raised is：Light irradiation time is that 14-16 is small
When, temperature be 20-26 DEG C, humidity is 50%-80%.
6. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
Stating the condition fed for the first time in the 5th step is：The raising ratio of ladybug and aphid is 1:100, feeding environment temperature is 13-
20 DEG C, the photoperiod is (6~10) L：(14~18) D, relative humidity is 60%-80%.
7. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
Stating the condition fed for the second time in the 5th step is：The raising ratio of ladybug and aphid is 1:100, feeding environment temperature is 13-
20 DEG C, the photoperiod is (6~10) L：(14~18) D, relative humidity is 60%-80%.
8. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：Institute
It is 35-45 days to state the time interval that nursing is fed with the last time each time in the 5th step.
9. aphid according to claim 1 and its predator large-scale breeding, storage method, it is characterised in that：The
The temperature of refrigerator is 4-6 DEG C in five steps.
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| CN107581163A (en) * | 2017-11-03 | 2018-01-16 | 福建省农业科学院植物保护研究所 | It is a kind of that the method for eating mite rove beetle in yellow angle is raised using Tetranychus cinnabarinus |
| CN107683828A (en) * | 2017-10-05 | 2018-02-13 | 福建省农业科学院植物保护研究所 | A kind of autotomy insect raising is with adopting ovum method |
| CN107926953A (en) * | 2017-11-28 | 2018-04-20 | 中国农业科学院棉花研究所 | Application of the S-Ethyl ethylthio sulfonate in the pollution insect for removing aphid |
| CN108353854A (en) * | 2018-01-26 | 2018-08-03 | 广西壮族自治区林业科学研究院 | A kind of dastarcus helophoroides ovum collecting device and collection method |
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| CN107683828A (en) * | 2017-10-05 | 2018-02-13 | 福建省农业科学院植物保护研究所 | A kind of autotomy insect raising is with adopting ovum method |
| CN107683828B (en) * | 2017-10-05 | 2021-05-18 | 福建省农业科学院植物保护研究所 | Self-disabled insect feeding and egg collecting method |
| CN107581163A (en) * | 2017-11-03 | 2018-01-16 | 福建省农业科学院植物保护研究所 | It is a kind of that the method for eating mite rove beetle in yellow angle is raised using Tetranychus cinnabarinus |
| CN107581163B (en) * | 2017-11-03 | 2021-05-14 | 福建省农业科学院植物保护研究所 | Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus |
| CN107926953A (en) * | 2017-11-28 | 2018-04-20 | 中国农业科学院棉花研究所 | Application of the S-Ethyl ethylthio sulfonate in the pollution insect for removing aphid |
| CN107926953B (en) * | 2017-11-28 | 2020-07-17 | 中国农业科学院棉花研究所 | Application of ethylicin in removing aphid-polluted insects |
| CN108353854A (en) * | 2018-01-26 | 2018-08-03 | 广西壮族自治区林业科学研究院 | A kind of dastarcus helophoroides ovum collecting device and collection method |
| CN111374095A (en) * | 2020-02-19 | 2020-07-07 | 四川省农业科学院植物保护研究所 | Cultivation method of predatory ladybug |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
| CN111264476B (en) * | 2020-03-12 | 2024-06-07 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg block collecting method and device |
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Application publication date: 20170524 |