CN105875507B - A kind of method of artificial mass production pest natural enemy - Google Patents
A kind of method of artificial mass production pest natural enemy Download PDFInfo
- Publication number
- CN105875507B CN105875507B CN201410201233.8A CN201410201233A CN105875507B CN 105875507 B CN105875507 B CN 105875507B CN 201410201233 A CN201410201233 A CN 201410201233A CN 105875507 B CN105875507 B CN 105875507B
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- China
- Prior art keywords
- raising
- snake gourd
- adult
- filter paper
- frankliniella occidentalis
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 18
- 235000008326 Trichosanthes anguina Nutrition 0.000 claims abstract description 62
- 244000078912 Trichosanthes cucumerina Species 0.000 claims abstract description 62
- 241000927584 Frankliniella occidentalis Species 0.000 claims abstract description 43
- 201000010099 disease Diseases 0.000 claims abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 10
- 230000000384 rearing effect Effects 0.000 claims abstract description 9
- 241000238876 Acari Species 0.000 claims abstract description 6
- 241000259672 Bolitobius cingulatus Species 0.000 claims abstract description 6
- 241001292007 Chrysopa Species 0.000 claims abstract description 6
- 241000244206 Nematoda Species 0.000 claims abstract description 6
- 241001414989 Thysanoptera Species 0.000 claims abstract description 6
- 230000003071 parasitic effect Effects 0.000 claims abstract description 6
- 241000255749 Coccinellidae Species 0.000 claims abstract description 5
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 21
- 108010000912 Egg Proteins Proteins 0.000 claims description 21
- 241000819999 Nymphes Species 0.000 claims description 21
- 210000004681 ovum Anatomy 0.000 claims description 21
- 241000382353 Pupa Species 0.000 claims description 18
- 238000009395 breeding Methods 0.000 claims description 10
- 230000001488 breeding effect Effects 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 7
- 210000001015 abdomen Anatomy 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 5
- 238000007598 dipping method Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 230000037406 food intake Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims 1
- 241000270295 Serpentes Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 244000053095 fungal pathogen Species 0.000 claims 1
- 230000002265 prevention Effects 0.000 abstract description 7
- 241000233866 Fungi Species 0.000 abstract description 5
- 244000062804 prey Species 0.000 abstract description 5
- 238000004064 recycling Methods 0.000 abstract description 3
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 231100000572 poisoning Toxicity 0.000 description 4
- 230000000607 poisoning effect Effects 0.000 description 4
- 238000011017 operating method Methods 0.000 description 2
- 241000578422 Graphosoma lineatum Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of methods of artificial mass production pest natural enemy, it is characterized in that under conditions of 22 DEG C 27 DEG C of temperature, humidity 50% one 80%, first with the artificial mass rearing Frankliniella occidentalis of snake gourd, the method for recycling the artificial mass production pest natural enemy of Frankliniella occidentalis, the pest natural enemy refers to Predatory Mites, predatism thrips, predatory bug, ladybug, Chrysopa, rove beetle, pathogenic nematode, disease fungus and parasitic wasp.Although present invention operation is more complex, cost is higher, the pest natural enemy produced using this method compares the same kind that other preys are produced, and the prevention of Frankliniella occidentalis can be adapted to faster in the prevention of Frankliniella occidentalis, to be conducive to the promotion of preventive effect.
Description
Technical field
The present invention relates to a kind of methods of artificial mass rearing Frankliniella occidentalis natural enemy, belong to modern agriculture biological control of insect pests
Field.
Background technology
Frankliniella occidentalis is important agricultural pests, and Natural Enemies have Predatory Mites, predatism thrips, predatory bug, wooden dipper
Worm, Chrysopa, rove beetle, pathogenic nematode, disease fungus and parasitic wasp.The raising of these natural enemies at present mainly utilizes non-west flower Ji
Horse produces as foodstuff, however the selection preference of the hostile Frankliniella occidentalis in day can be influenced by producing these natural enemies using other foodstuffs,
To influence the predation of the hostile Frankliniella occidentalis in day.
In order to cultivate the natural enemy of Frankliniella occidentalis prevention preferably, Frankliniella occidentalis is selected particularly must as the prey of its natural enemy
It wants.
Invention content
The object of the present invention is to provide a kind of method of artificial mass production pest natural enemy, the evil produced using this method
Worm natural enemy compares the same kind that other preys are produced, and can adapt to Frankliniella occidentalis faster in the prevention of Frankliniella occidentalis
Prevention, to be conducive to the promotion of preventive effect.
The present invention's is manually largely raised first with snake gourd under conditions of 22 DEG C -27 DEG C of temperature, humidity 50%-80%
Frankliniella occidentalis is supported, the method for recycling the artificial mass production pest natural enemy of Frankliniella occidentalis, the pest natural enemy refers to Predatory Mites, catches
Feeding habits thrips, predatory bug, ladybug, Chrysopa, rove beetle, pathogenic nematode, disease fungus and parasitic wasp.
The method using the artificial mass rearing Frankliniella occidentalis of snake gourd, concrete operation step are：
1) preparation of snake gourd：Select it is fresh have no mechanical damage, no disease and pests harm harm snake gourd, first use hundred poisoning thimerosals soak
Bubble 20 minutes, is then cleaned 2-3 times with clear water, then is impregnated 10 minutes with clear water, then dries, snake gourd is put into bee again after drying
It impregnates 1 minute or so in mulse, is dried again after taking-up, in case raising adult is used.
2) preparation of container is raised：Raising container is the glass preserving jar of an opening uncovered, can body be it is cylindrical,
Bottleneck is circle, and bottle height 10-15cm, bottleneck diameter 5-10cm need to be first with hundred poisoning medicining liquid dippings 20 using preceding can
Minute, it cleans after drying, it is for use that the filter paper with the sizes such as bottom of bottle is placed on can bottom of bottle with tweezers.
3) raising of Frankliniella occidentalis includes the raising of adult, the collection of ovum, the raising of nymph, the raising of pupa, then arrives adult
Raising links, required Frankliniella occidentalis worm state can be taken out from the links of raising as needed.Each link is specific
Operating procedure is：
The raising of a adults：First ready 4-6 roots snake gourd is put into raising container, then Frankliniella occidentalis adult is accessed
It raises in container, is finally sealed and raised with the fly net of 300 mesh, the raising of adult is to replace within every 1-3 days primary fresh snake gourd,
After every time taking the snake gourd with worm's ovum only, need add fresh snake gourd into imago breeding container again.
The collection of b ovum：After imago breeding 1-3 days, snake gourd (being the ovum containing Frankliniella occidentalis on snake gourd) is fetched into raising
Vessel port blows the adult on snake gourd off with ear washing bulb, and adult still stays in imago breeding container, waits for the adult drying on snake gourd
After net, the snake gourd with ovum is put into new raising container, at the same add into new raising container several it is fresh, ready
Snake gourd marks raising date of operation after sealing mouth on raising container.
The raising of c nymphs：Ovum can be changed to nymph in new raising container after development, and nymph is divided into two ages：
One age nymph (development 1-3 days), which just hatches after body colour is transparent when coming, becomes white, becomes yellow before husking；Two age nymphs (hair
Educate 4-7 days) it is very active, food ingestion is 3 times of 1 age nymph, 2 age nymph wax yellows.During raising nymph, it should be noted
Dry snake gourd is taken out, and the filter paper of can bottom is changed into new filter paper, is respectively put into according to nymphal instars difference appropriate new
Fresh snake gourd continues to raise after being sealed with one layer of fly net, if the can that can and filter paper are dirty or renew if having mould and
Filter paper.
The raising of d pupas：Pupa includes propupa and pupa：Propupa (development 8-10 days) body white, body shorten, wing bud occur, touch
Angle is short and thick, holds up forward, can slowly be moved to gap, shady place, not feeding or few feeding；Pupa (development 11-12 days) is compared with propupa
There are many wing bud length, and length is more than abdomen half, almost grows to abdomen end, the teges of adult occurs, and feeler is elongated, is located at the back of the body
Face, not feeding or few feeding.It should be noted during raising pupa and take out dry snake gourd, and the filter paper of can bottom is changed
The filter paper of Cheng Xin is respectively put into proper amount of fresh snake gourd according to worm state difference, then is sealed and raised with fly net, if can and filter paper become
Can and filter paper dirty or that there is mould then to renew.
The raising of e adults：After the snake gourd for carrying ovum in former adult tank is taken out, then will 12-14 days new adults of development
It is merged into former adult tank, and adds fresh snake gourd, with continuing to raise after the fly net sealing of 300 mesh, if can and filter
Paper is dirty or the can and filter paper that have mould then to renew；
The method using the artificial mass production pest natural enemy of Frankliniella occidentalis refers to choosing after mass rearing Frankliniella occidentalis
The Frankliniella occidentalis worm state for taking needs is put into the production for pest natural enemy under conditions of suitable pest natural enemy production, under this condition
Other preys of pest natural enemy can be contained.
Although present invention operation is more complex, cost is higher, using the pest natural enemy that the present invention produces, not only it is more suitable for
The prevention of Frankliniella occidentalis, and the quality of these natural enemies prevention Frankliniella occidentalis can also be improved.
Specific implementation mode
The method for breeding of 1 Frankliniella occidentalis of embodiment：
Under conditions of 22 DEG C -27 DEG C of temperature, humidity 50%-80%, first with the artificial mass rearing Frankliniella occidentalis of snake gourd,
The method for recycling the artificial mass production pest natural enemy of Frankliniella occidentalis, the pest natural enemy refer to Predatory Mites, predatism thrips, catch
Feeding habits stinkbug class, ladybug, Chrysopa, rove beetle, pathogenic nematode, disease fungus and parasitic wasp.
The method using the artificial mass rearing Frankliniella occidentalis of snake gourd, concrete operation step are：
1) preparation of snake gourd：Select it is fresh have no mechanical damage, no disease and pests harm harm snake gourd, first use hundred poisoning thimerosals soak
Bubble 20 minutes, is then cleaned 2-3 times with clear water, then is impregnated 10 minutes with clear water, then dries, snake gourd is put into bee again after drying
It impregnates 1 minute or so in mulse, is dried again after taking-up, in case raising adult is used.
2) preparation of container is raised：Raising container is the glass preserving jar of an opening uncovered, can body be it is cylindrical,
Bottleneck is circle, and bottle height 10-15cm, bottleneck diameter 5-10em need to be first with hundred poisoning medicining liquid dippings 20 using preceding can
Minute, it cleans after drying, it is for use that the filter paper with the sizes such as bottom of bottle is placed on can bottom of bottle with tweezers.
3) raising of Frankliniella occidentalis includes the raising of adult, the collection of ovum, the raising of nymph, the raising of pupa, then arrives adult
Raising links, required Frankliniella occidentalis worm state can be taken out from the links of raising as needed.Each link is specific
Operating procedure is：
The raising of a adults：First ready 4-6 roots snake gourd is put into raising container, then Frankliniella occidentalis adult is accessed
It raises in container, is finally sealed and raised with the fly net of 300 mesh, the raising of adult is to replace within every 1-3 days primary fresh snake gourd,
After every time taking the snake gourd with worm's ovum only, need add fresh snake gourd into imago breeding container again.
The collection of b ovum：After imago breeding 1-3 days, snake gourd (being the ovum containing Frankliniella occidentalis on snake gourd) is fetched into raising
Vessel port blows the adult on snake gourd off with ear washing bulb, and adult still stays in imago breeding container, waits for the adult drying on snake gourd
After net, the snake gourd with ovum is put into new raising container, at the same add into new raising container several it is fresh, ready
Snake gourd marks raising date of operation after sealing mouth on raising container.
The raising of c nymphs：Ovum can be changed to nymph in new raising container after development, and nymph is divided into two ages：
One age nymph (development 1*3 days), which just hatches after body colour is transparent when coming, becomes white, becomes yellow before husking；Two age nymphs (hair
Educate 4-7 days) it is very active, food ingestion is 3 times of 1 age nymph, 2 age nymph wax yellows.During raising nymph, it should be noted
Dry snake gourd is taken out, and the filter paper of can bottom is changed into new filter paper, is respectively put into according to nymphal instars difference appropriate new
Fresh snake gourd continues to raise after being sealed with one layer of fly net, if the can that can and filter paper are dirty or renew if having mould and
Filter paper.
The raising of d pupas：Pupa includes propupa and pupa：Propupa (development 8-10 days) body white, body shorten, wing bud occur, touch
Angle is short and thick, holds up forward, can slowly be moved to gap, shady place, not feeding or few feeding；Pupa (development 11-12 days) is compared with propupa
There are many wing bud length, and length is more than abdomen half, almost grows to abdomen end, the teges of adult occurs, and feeler is elongated, is located at the back of the body
Face, not feeding or few feeding.It should be noted during raising pupa and take out dry snake gourd, and the filter paper of can bottom is changed
The filter paper of Cheng Xin is respectively put into proper amount of fresh snake gourd according to worm state difference, then is sealed and raised with fly net, if can and filter paper become
Can and filter paper dirty or that there is mould then to renew.
The raising of e adults：After the snake gourd for carrying ovum in former adult tank is taken out, then will 12-14 days new adults of development
It is merged into former adult tank, and adds fresh snake gourd, with continuing to raise after the fly net sealing of 300 mesh, if can and filter
Paper is dirty or the can and filter paper that have mould then to renew；
The production of 2 pest natural enemy of embodiment
The method using the artificial mass production pest natural enemy of Frankliniella occidentalis refers to choosing after mass rearing Frankliniella occidentalis
The Frankliniella occidentalis worm state for taking needs, is put into suitable Predatory Mites, predatism thrips, predatory bug, ladybug, Chrysopa, rove beetle, disease
It is used for the production of these pest natural enemies under conditions of former nematode, disease fungus and parasitic wasp production, can contain under this condition harmful
Other preys of worm natural enemy, can not also contain other preys.
The condition of the suitable pest natural enemy production refers to according to the hostile raising container in different days, humiture and various aspects
Demand is different, and suitable environment is provided to using Frankliniella occidentalis production pest natural enemy.
Claims (1)
1. a kind of method of artificial mass production pest natural enemy, it is characterized in that in 22 DEG C -27 DEG C of temperature, humidity 50%-80%
Under the conditions of, first with the artificial mass rearing Frankliniella occidentalis of snake gourd, recycle the side of the artificial mass production pest natural enemy of Frankliniella occidentalis
Method, the pest natural enemy refer to Predatory Mites, predatism thrips, predatory bug, ladybug, Chrysopa, rove beetle, pathogenic nematode, disease
Fungal pathogens and parasitic wasp, the method using the artificial mass rearing Frankliniella occidentalis of snake gourd, concrete operation step are：
1) preparation of snake gourd：Select it is fresh have no mechanical damage, no disease and pests harm harm snake gourd, first use hundred kill medicining liquid dippings 20
Minute, it is then cleaned 2-3 times with clear water, then impregnated 10 minutes with clear water, then dries, snake gourd is put into hydromel again after drying
It is middle to impregnate 1 minute or so, it is dried again after taking-up, in case raising adult is used；
2) preparation of container is raised：Raising container is the glass preserving jar of an opening uncovered, and can body is cylindrical, bottleneck
For circle, bottle height 10-15cm, bottleneck diameter 5-10cm first need to kill medicining liquid dipping 20 minutes with hundred using preceding can, wash
It is net dry after, it is for use that the filter paper with the sizes such as bottom of bottle is placed on can bottom of bottle with tweezers；
3) raising of Frankliniella occidentalis includes the raising of adult, the collection of ovum, the raising of nymph, the raising of pupa, then the feeding to adult
Links are supported, take out required Frankliniella occidentalis worm state, each link concrete operations step from the links of raising as needed
Suddenly it is：
The raising of a adults：First ready 4-6 roots snake gourd is put into raising container, then Frankliniella occidentalis adult is accessed and is raised
It in container, is finally sealed and is raised with the fly net of 300 mesh, the raising of adult is to replace within every 1-3 days primary fresh snake gourd, i.e., often
It is secondary by the snake gourd with worm's ovum take it is net after, need add fresh snake gourd into imago breeding container again；
The collection of b ovum：After imago breeding 1-3 days, the snake gourd containing Frankliniella occidentalis ovum is fetched into raising vessel port, uses ear washing bulb
Blow the adult on snake gourd off, adult still stays in imago breeding container, after the adult on snake gourd blows clean, by the snake with ovum
Beans are put into new raising container, while adding several fresh, ready snake gourds into new raising container, seal after mouth
Raise mark raising date of operation on container；
The raising of c nymphs：Ovum can be changed to nymph in new raising container after development, and nymph is divided into two ages：One age
Nymph, which just hatches after body colour is transparent when coming, becomes white, becomes yellow before husking；Two age nymphs are very active, food ingestion 1
3 times of age nymph, 2 age nymph wax yellows should be noted that snake gourd that will be dry takes out during raising nymph, and by can
The filter paper of bottom changes new filter paper into, and proper amount of fresh snake gourd is respectively put into according to nymphal instars difference, after being sealed with one layer of fly net
Continue to raise, if the can and filter paper that can and filter paper are dirty or renew if having mould；
The raising of d pupas：Pupa includes propupa and pupa：Preceding pupal cell white, body shorten, and wing bud occur, and feeler is short and thick, holds up forward,
It can slowly be moved to gap, shady place, not feeding or few feeding；Pupa is many compared with propupa wing bud length, and length is more than abdomen half, several
Long the teges of adult occur to abdomen end, feeler is elongated, is located at the back side, not feeding or few feeding；In the mistake of raising pupa
It should be noted in journey and take out dry snake gourd, and the filter paper of can bottom is changed into new filter paper, be respectively put into according to worm state difference
Proper amount of fresh snake gourd, then sealed and raised with fly net, if can and filter paper be dirty or the can renewed if having mould and filter
Paper；
The raising of e adults：After the snake gourd for carrying ovum in former adult can is taken out, then will 12-14 days new adults of development
It is merged into former adult can, and adds fresh snake gourd, with continuing to raise after the fly net sealing of 300 mesh, if can
With filter paper is dirty or the can and filter paper that have mould then to renew.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410201233.8A CN105875507B (en) | 2014-05-14 | 2014-05-14 | A kind of method of artificial mass production pest natural enemy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410201233.8A CN105875507B (en) | 2014-05-14 | 2014-05-14 | A kind of method of artificial mass production pest natural enemy |
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| Publication Number | Publication Date |
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| CN105875507A CN105875507A (en) | 2016-08-24 |
| CN105875507B true CN105875507B (en) | 2018-08-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410201233.8A Expired - Fee Related CN105875507B (en) | 2014-05-14 | 2014-05-14 | A kind of method of artificial mass production pest natural enemy |
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| CN110447604B (en) * | 2019-09-17 | 2020-07-28 | 中国农业科学院植物保护研究所 | Method for improving feeding effect of arma chinensis by adding alternative host plant |
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| CN102640729B (en) * | 2012-04-06 | 2014-02-26 | 山东省农业科学院植物保护研究所 | Method for breeding frankliniella occidentalis |
| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
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