CN104938429B - A kind of method for raising Orius - Google Patents
A kind of method for raising Orius Download PDFInfo
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- CN104938429B CN104938429B CN201510373964.5A CN201510373964A CN104938429B CN 104938429 B CN104938429 B CN 104938429B CN 201510373964 A CN201510373964 A CN 201510373964A CN 104938429 B CN104938429 B CN 104938429B
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- 241001635529 Orius Species 0.000 title claims abstract description 113
- 238000000034 method Methods 0.000 title claims abstract description 58
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 98
- 235000013339 cereals Nutrition 0.000 claims abstract description 46
- 238000011282 treatment Methods 0.000 claims abstract description 38
- 238000003763 carbonization Methods 0.000 claims abstract description 35
- 230000017448 oviposition Effects 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 15
- 125000006850 spacer group Chemical group 0.000 claims abstract description 12
- 210000004681 ovum Anatomy 0.000 claims description 99
- 102000002322 Egg Proteins Human genes 0.000 claims description 68
- 108010000912 Egg Proteins Proteins 0.000 claims description 68
- 238000009395 breeding Methods 0.000 claims description 18
- 230000001488 breeding effect Effects 0.000 claims description 18
- 230000008859 change Effects 0.000 claims description 18
- 238000005286 illumination Methods 0.000 claims description 18
- 241000819999 Nymphes Species 0.000 claims description 17
- 230000012447 hatching Effects 0.000 claims description 17
- 241000209140 Triticum Species 0.000 claims description 14
- 235000021307 Triticum Nutrition 0.000 claims description 14
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 239000003905 agrochemical Substances 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 238000007664 blowing Methods 0.000 claims description 5
- 229940098396 barley grain Drugs 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims description 2
- 230000001052 transient effect Effects 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000002224 dissection Methods 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 18
- 241000578422 Graphosoma lineatum Species 0.000 description 11
- 235000013305 food Nutrition 0.000 description 8
- 241001635552 Anthocoridae Species 0.000 description 7
- 229920000742 Cotton Polymers 0.000 description 7
- 230000008569 process Effects 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 230000003031 feeding effect Effects 0.000 description 4
- 238000012376 hot air sterilization Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002791 soaking Methods 0.000 description 4
- 241000266501 Ormosia ormondii Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000209219 Hordeum Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000004920 integrated pest control Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241001635523 Orius sauteri Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses a kind of method for raising Orius, which is using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the cereal kernel of Jing carbonization treatments as main spacer medium, the method that humidity raises Orius in container is raised with the combination control of " cereal kernel --- fresh beanpod ".In practice, the advantages of this method has easy to operate, Orius developmental rate ideal, life-span length, the death rate are low, adult pick-up rate high, Adult worms producting eggs amount is big.
Description
Technical field
The invention belongs to integrated pest management field and predatory natural enemy insect breeding technology, are related to a kind of Orius
Method for breeding.
Background technology
Orius Oriussauteri be it is a kind of be distributed extremely extensive natural enemy insect in northern China, nymph and into
Worm can prey on various small-sized agriculture and forestry injurious insects such as thrips, aphid, aleyrodid, tetranychid in a large number, can also prey on the ovum of lepidoptera pest
(such as bollworm, diamondback moth etc.), has great using value in integrated pest management.Therefore many scholars are on the basis of forefathers
On carried out a large amount of basic research, especially in terms of artificial feeding.Current China Orius artificial diet technique and Jing
Test also very not enough, which is still at an early stage as biological and ecological methods to prevent plant disease, pests, and erosion agent, apart from large-scale production and using also sizable gap.
Orius raise vital two conditions on a large scale：On the one hand it is feed, uses Current Domestic more
The small arthropod such as life food, mainly aphid, thrips, tetranychid；On the other hand it is oviposition substrate, adopts Current Domestic more
With the live plant bud stem (such as bean sprouts, kidney bean seedling stem etc.) that children is tender.Either feed or oviposition substrate all have working condition
Height, high cost, yield poorly, long the production cycle the shortcomings of.Additionally, in high-density rearing, Orius have kills each other
Habit, such as imappropriate process, feeding effect will certainly be affected.The humid control raised in container is also most important, is dried meeting
Orius ovum and the low instar nymphs death rate is caused to be significantly increased；But humidity is too high, to raise and the globule can be condensed on chamber wall,
Orius are easily stuck, or even are drowned, while high humility is also easy to cause feed and oviposition substrate to rot to go mouldy, raise
Long mildew on foster chamber wall.There is Orius developmental rate, life-span, the death rate, adult to obtain with conventional husbandry methods
The drawback such as rate, Adult worms producting eggs amount be not ideal enough.These drawbacks limit the extensive raising of Orius in all its bearings, system
About the industrialization of Orius.
The content of the invention：
For many technical problems present in existing Orius artificial feeding, the purpose of invention is that solution is above-mentioned
One of technical problem, further for the extensive raising that can preferably develop Orius, promotes its industrialization, invention
People has invented one kind using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with Jing through long-term experiment
The cereal kernel of carbonization treatment raises container with the combination control of " cereal kernel --- fresh beanpod " as main spacer medium
The method that interior humidity raises Orius.
A kind of method for raising Orius, using gelechiid ova as main feed, using kidney bean beanpod as main spawning
Matrix, using the cereal kernel of Jing carbonization treatments as main spacer medium, with the combination control of " cereal kernel --- fresh beanpod "
System is raised humidity in container and raises Orius.
Preferably, the method for breeding is mainly included the following steps that：
(1) hatching of Orius ovum
During the cereal kernel that Jing carbonization treatments are crossed is placed in closing but ventilative raising tank, take with Orius ovum
Kidney bean beanpod is placed on cereal kernel, is hatched；
(2) raising of Orius nymph and adult
Once it was found that there is Orius nymph to hatch, fresh gelechiid ova is uniformly fed to raising in tank, and every 1-2d
Feed once fresh gelechiid ova；Such as find that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, change fresh kidney bean
Beanpod, and make the kidney bean beanpod number in raising tank remain at 8-10 roots；
(3) collection of Orius ovum
Once it was found that the Orius for raising 8-12% in tank turn into adult, all kidney bean beans are taken out every 1-3d
Pod, while changing fresh kidney bean beanpod, the kidney bean beanpod number raised in tank remains at 8-10 roots；With Orius ovum
Kidney bean beanpod can be directly used for breeding the next generation, or low temperature is temporarily stored.
Preferably, in the step (1), environment of hatching temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is
16L:8D；Step (2) the feeding environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D；The step
Suddenly (3) egg-laying environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D.
Preferably, the control methods of the environment of hatching humidity are：The cereal kernel that Jing carbonization treatments are crossed is placed in into glass
Raise pot bottom, keep raising the fresh kidney bean beanpod number in tank all the time in 8-10 roots, using it is this " cereal kernel --- it is fresh
The combination of beanpod " come control raise tank in humidity.
Preferably, using being front both needed to through hot air sterilization, sterilising conditions are the raising tank tool：90 DEG C of temperature, time
10h。
Preferably, the condition of the cereal kernel carbonization treatment is：200 DEG C of temperature, time 5h；Cereal kernel carbonization treatment
Afterwards, need to spread out in room temperature, clean environment blowing 5h, the cereal kernel can for wheat seed, barley grain, corn kernel or
Soya bean seed.
Preferably, the initial feeding volume of the gelechiid ova is 0.5-1.0g, as Orius age gradually increases, single
Gelechiid ova feeding volume also progressively increases to 1.0-2.0g therewith.
Preferably, the kidney bean beanpod must be fresh undamaged, and from forbidding making not in contact with crossing China Ministry of Agriculture
Agricultural chemicals, using front, after need to soaking 30min in the hydrogen peroxide solution that mass-volume concentration is 3%, room temperature is dried.
Preferably, the kidney bean beanpod of taking-up is checked whether there is into Orius ovum under anatomical lens in the step (3)
And count, under Orius ovum anatomical lens, inspection method is：Orius often originate in ovum in kidney bean beanpod edge tissues,
The marginal swell of the outer color that only shows money or valuables one carries unintentionally, intermediate recess, recessed central have the circular ovum operculum being raised slightly.
Preferably, the temporary transient condition of storage of the Orius ovum low temperature is：Temperature 2-5 DEG C, humidity 90%, but when storing
Between no more than 100d.
Specifically, Orius method for breeding of the present invention is mainly included the following steps that：
(1) hatching of Orius ovum
The cereal kernel that 20-30g Jing carbonization treatments are crossed is placed in into the glass that volume is 1500mL and raises pot bottom, take 8-10
Kidney bean beanpod of the root with Orius ovum is placed on cereal kernel, cotton is tied up in tank mouth with rubber band.Environment of hatching temperature
Spend for 25-28 DEG C, humidity is 60-70%, and illumination condition is 16L:8D.See whether that Orius nymph hatches daily.
(2) raising of Orius nymph and adult
Once it was found that there is Orius nymph to hatch, fresh gelechiid ova is uniformly fed to raising in tank immediately, and every
1-2d feeds once fresh gelechiid ova.Such as find that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, change fresh
Kidney bean beanpod, and make the kidney bean beanpod number in raising tank remain at 8-10 roots.Feeding environment temperature is 25-28 DEG C, humidity
For 60-70%, illumination condition is 16L:8D.
(3) collection of Orius ovum
Once it was found that the Orius for raising in tank about 10% turn into adult, then change all wiltings withered and yellow or rotten
The kidney bean beanpod for going mouldy (include slightly wilting withered and yellow or rotting to go mouldy), and all kidney bean beanpods are taken out every 2d, while changing
Fresh kidney bean beanpod, the kidney bean beanpod number raised in tank remain at 8-10 roots.Egg-laying environment temperature is 25-28 DEG C, humidity
For 60-70%, illumination condition is 16L:8D.The kidney bean beanpod of taking-up is checked whether there is into Orius ovum simultaneously under anatomical lens
Count.Kidney bean beanpod with Orius ovum can be directly used for breeding the next generation, also can low temperature temporarily store.
(4) spacer medium
The cereal kernel that 20-30g Jing carbonization treatments are crossed is placed in into glass and raises pot bottom, as Orius in raising
Spacer medium.
(5) raise humidity regulation in tank
The cereal kernel that 20-30g Jing carbonization treatments are crossed is placed in into glass and raises pot bottom, keep raising the fresh rue in tank
Beans beanpod number controls to raise humidity in tank using the combination of this " cereal kernel --- fresh beanpod " all the time in 8-10 roots.
Wherein, the Orius ovum described in step (1) often raises the injected volume of tank less than 800.
Step (1), (2), (3) described tank of raising are same raising tank, i.e., in whole feeding process, generally not
Need to change and raise tank.
This method can be prevented effectively from the working condition height of feed and oviposition substrate in conventional husbandry methods, high cost, product
Measure the drawbacks such as low, long the production cycle.This method is using gelechiid ova as main feed, the production technology maturation of gelechiid ova, cost
Low, yield is high, with short production cycle；And main oviposition substrate, kidney bean beanpod is commercially available throughout the year, saves conventional husbandry
The operation of oviposition substrate is individually produced in method.This method is little as East Asia in raising using the cereal kernel that Jing carbonization treatments are crossed
The spacer medium of flower stinkbug, on the one hand can artificially produce a large amount of spaces and hide oneself for Orius, certainly mutually residual so as to reduce which
The probability for killing；On the other hand, the cereal kernel of carbonization treatment is substantially free of nutriment, even if do not taken food by Orius
Gelechiid ova has an opportunity to hatch gelechiid larva, can not find the food of abundance, grows in tank is raised so as to avoid gelechiid
Development, disturbs the raising operation of Orius.This method controls to raise using the combination of " cereal kernel --- fresh beanpod "
Humidity in foster tank, on the one hand using the water absorbing capacity of cereal kernel come prevent raise top tank structure condense out the liquid globule affect East Asia
Minute pirate bugs activity；On the other hand gaseous state moisture is discharged using fresh beanpod prevent to raise in tank that humidity is too low to cause East Asia little Hua
The death of stinkbug.During Orius being raised using this method, need not generally change raising tank, Ke Yiyou
Effect simplifies operating procedure.In practice, this method has easy to operate, Orius developmental rate ideal, life-span length, death
Rate is low, adult pick-up rate height, Adult worms producting eggs phase length, big Adult worms producting eggs amount the advantages of.
The additional aspect and advantage of the present invention will be set forth in part in the description, and partly will become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Description of the drawings
Fig. 1 is 1 egg laying amount of embodiment；
Fig. 2 is 2 egg laying amount of embodiment；
Fig. 3 is 3 egg laying amount of embodiment.
Specific embodiment
Below in conjunction with instantiation, the present invention is described in detail.
Embodiment 1
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with the wheat seed of Jing carbonization treatments
Grain raises humidity raising East Asia in container with the combination control of " cereal kernel --- fresh beanpod " little as main spacer medium
Flower stinkbug, method for breeding comprise the following steps：
(1) prepare before raising
The all utensils used in raising are through hot air sterilization, including 1500mL glass raises tank, 225cm2Square cotton
Cloth, rubber band and related tool etc., sterilising conditions are：90 DEG C of temperature, time 10h.
Taking 35g wheat seeds carries out carbonization treatment, and the condition of carbonization treatment is：200 DEG C of temperature, time 5h.Carbonization treatment
Afterwards, spread blowing 5h in room temperature, clean environment out.
From the fresh not damaged of food market by-batch purchase from the kidney bean beanpod not in contact with violated agricultural chemicals, sterilization treatment is carried out, is gone out
Bacterium method is：After soaking 30min in the hydrogen peroxide solution that mass-volume concentration is 3%, room temperature is dried.
(2) hatching of Orius ovum
The wheat seed of Jing carbonization treatments is placed in into glass and raises pot bottom, 8 are taken with Orius ovum (totally 338
Grain) kidney bean beanpod be placed on wheat seed, cotton is tied up in tank mouth with rubber band.
Environment of hatching temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.See whether that East Asia is little daily
The nymph hatching of flower stinkbug.
(3) raising of Orius nymph and adult
At the 4th day, in finding to raise tank, there is Orius nymph to hatch, uniformly feed into each raising tank immediately
Fresh gelechiid ova 0.5g.Fresh gelechiid ova is fed once every 2d afterwards, as Orius age gradually increases, single wheat
Moth ovum feeding volume also progressively increases to 2.0g therewith.
At the 7th day, finding to raise had part kidney bean beanpod wilting withered and yellow in tank, that is, change fresh kidney bean beanpod.This is latter
Denier finds that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, change fresh kidney bean beanpod, and make in raising tank
Kidney bean beanpod number remains at 8.
Feeding environment temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
In fortnight, it is found that the Orius for raising in tank about 10% turn into adult, then change all wiltings withered
The yellow or kidney bean beanpod for going mouldy (include slightly wilting withered and yellow or rotting to go mouldy) that rots, and all kidney bean beanpods are taken out every 2d,
Change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 8.
Egg-laying environment temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether there is into Orius ovum under anatomical lens and is counted, method is：East Asia is little
Stinkbug often originates in ovum in kidney bean beanpod edge tissues flower, and the marginal swell of the color that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have slightly
The circular ovum operculum of protuberance.Total egg laying amount is 1401 (see Fig. 1).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) is obtained can be directly used for breeding the next generation, method synchronization
Suddenly (2).
(6) low temperature of filial generation Orius ovum is temporarily stored
The kidney bean beanpod with Orius ovum that step (4) is obtained can low temperature temporarily store, condition of storage is：Temperature
2 DEG C, humidity 90%, storage time are less than 100d.
Embodiment 2
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with the barley seed of Jing carbonization treatments
Grain raises humidity raising East Asia in container with the combination control of " cereal kernel --- fresh beanpod " little as main spacer medium
Flower stinkbug, method for breeding comprise the following steps：
(1) prepare before raising
The all utensils used in raising are through hot air sterilization, including 1500mL glass raises tank, 225cm2Square cotton
Cloth, rubber band and related tool etc., sterilising conditions are：90 DEG C of temperature, time 10h.
Taking 30g barley grains carries out carbonization treatment, and the condition of carbonization treatment is：200 DEG C of temperature, time 5h.Carbonization treatment
Afterwards, spread blowing 5h in room temperature, clean environment out.
From the fresh not damaged of food market by-batch purchase from the kidney bean beanpod not in contact with excessively violated agricultural chemicals, sterilization treatment is carried out,
Sterilizing methods are：After soaking 30min in the hydrogen peroxide solution that mass-volume concentration is 3%, room temperature is dried.
(2) hatching of Orius ovum
The barley grain of Jing carbonization treatments is placed in into glass and raises pot bottom, 10 are taken with Orius ovum (totally 526
Grain) kidney bean beanpod be placed on barley grain, cotton is tied up in tank mouth with rubber band.
Environment of hatching temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.East Asia little Hua has been seen whether daily
Stinkbug nymph hatches.
(3) raising of Orius nymph and adult
At the 3rd day, in finding to raise tank, there is Orius nymph to hatch, uniformly feed into each raising tank immediately
Fresh gelechiid ova 0.5g.Fresh gelechiid ova is fed once every 1d afterwards, as Orius age gradually increases, single wheat
Moth ovum feeding volume also progressively increases to 2.0g therewith.
At the 7th day, finding to raise had part kidney bean beanpod wilting withered and yellow in tank, that is, change fresh kidney bean beanpod.This is latter
Denier finds that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, change fresh kidney bean beanpod, and make in raising tank
Kidney bean beanpod number remains at 10.
Feeding environment temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
At the 12nd day, it is found that the Orius for raising in tank about 10% turn into adult, then change all wiltings withered
The yellow or kidney bean beanpod for going mouldy (include slightly wilting withered and yellow or rotting to go mouldy) that rots, and all kidney bean beanpods are taken out every 2d,
Change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 10.
Egg-laying environment temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether there is into Orius ovum under anatomical lens and is counted, method is：East Asia is little
Stinkbug often originates in ovum in kidney bean beanpod edge tissues flower, and the marginal swell of the color that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have slightly
The circular ovum operculum of protuberance.Total egg laying amount is 2033 (see Fig. 2).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) is obtained can be directly used for breeding the next generation, method synchronization
Suddenly (2).
(6) low temperature of filial generation Orius ovum is temporarily stored
The kidney bean beanpod with Orius ovum that step (4) is obtained can low temperature temporarily store, condition of storage is：Temperature
4 DEG C, humidity 90%, storage time are less than 100d.
Embodiment 3
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with the Corn Seeds of Jing carbonization treatments
Grain raises humidity raising East Asia in container with the combination control of " cereal kernel --- fresh beanpod " little as main spacer medium
Flower stinkbug, method for breeding comprise the following steps：
(1) prepare before raising
The all utensils used in raising are through hot air sterilization, including 1500mL glass raises tank, 225cm2Square cotton
Cloth, rubber band and related tool etc., sterilising conditions are：90 DEG C of temperature, time 10h.
Taking 30g corn kernels carries out carbonization treatment, and the condition of carbonization treatment is：200 DEG C of temperature, time 5h.Carbonization treatment
Afterwards, spread blowing 5h in room temperature, clean environment out.
From the fresh not damaged of food market by-batch purchase from the kidney bean beanpod not in contact with violated agricultural chemicals, sterilization treatment is carried out, is gone out
Bacterium method is：After soaking 30min in the hydrogen peroxide solution that mass-volume concentration is 3%, room temperature is dried.
(2) hatching of Orius ovum
The corn kernel of Jing carbonization treatments is placed in into glass and raises pot bottom, 10 are taken with Orius ovum (totally 684
Grain) kidney bean beanpod be placed on corn kernel, cotton is tied up in tank mouth with rubber band.
Environment of hatching temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.East Asia little Hua has been seen whether daily
Stinkbug nymph hatches.
(3) raising of Orius nymph and adult
At the 3rd day, in finding to raise tank, there is Orius nymph to hatch, uniformly feed into each raising tank immediately
Fresh gelechiid ova 0.5g.Fresh gelechiid ova is fed once every 1d afterwards, as Orius age gradually increases, single wheat
Moth ovum feeding volume also progressively increases to 2.0g therewith.
At the 6th day, finding to raise in tank had part kidney bean beanpod to have the trend of going mouldy of rotting, that is, change fresh kidney bean beanpod.
Hereafter once finding that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, fresh kidney bean beanpod is changed, and makes raising
Kidney bean beanpod number in tank remains at 10.
Feeding environment temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
At the 12nd day, it is found that the Orius for raising in tank about 10% turn into adult, then change all wiltings withered
The yellow or kidney bean beanpod for going mouldy (include slightly wilting withered and yellow or rotting to go mouldy) that rots, and all kidney bean beanpods are taken out every 2d,
Change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 10.
Egg-laying environment temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether there is into Orius ovum under anatomical lens and is counted, method is：East Asia is little
Stinkbug often originates in ovum in kidney bean beanpod edge tissues flower, and the marginal swell of the color that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have slightly
The circular ovum operculum of protuberance.Total egg laying amount is 2546 (see Fig. 3).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) is obtained can be directly used for breeding the next generation, method synchronization
Suddenly (2).
(6) low temperature of filial generation Orius ovum is temporarily stored
The kidney bean beanpod with Orius ovum that step (4) is obtained can low temperature temporarily store, condition of storage is：Temperature
5 DEG C, humidity 90%, storage time are less than 100d.
Comparative example 1
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, do not use any material as every
From medium, the combination of " cereal kernel --- fresh beanpod " is not used to control to raise humidity raising Orius in container.Press
Raised according to the Orius method for breeding described in embodiment 1.
Embodiment 1 is compared with comparative example 1, it is found that embodiment 1 is good compared with the feeding effect of comparative example 1.
(1) raise humidity in tank
In whole feeding process, embodiment 1 is raised, and Orius can be on inwall certainly
By creeping；And comparative example 1 to be raised and condensed a large amount of globules on top tank structure, a large amount of Orius are clung by the globule on inwall,
Activity is extremely restricted, or even part is drowned because body is fully wrapped around by the globule.
This method controls to raise humidity in tank using the combination of " cereal kernel --- fresh beanpod ", on the one hand using paddy
The water absorbing capacity of thing seed is movable to prevent raising top tank structure from condensing sweating impact Orius；On the other hand using fresh
Beanpod discharges gaseous state moisture and prevents to raise the too low death for causing Orius of humidity in tank.
(2) mildew putrefactive phenomenon
In whole feeding process, mildew on the raising top tank structure of embodiment 1, is had no, kidney bean beanpod rots；And
Find 2 pieces of mildews on the raising top tank structure of comparative example 1 altogether, area about 4.5cm altogether2, have 7 kidney bean beanpods and occur in various degree
Rot.
(3) Orius are killed each other phenomenon
Orius in embodiment 1 can be between wheat seed space hide oneself, it is only a small amount of because of death of killing each other；
And in comparative example 1 because kill each other death Orius quantity be significantly more than embodiment 1, and if death mostly be low age
Worm.
(4) input and output rate
" narrow sense " input and output rate of embodiment 1 and comparative example 1 is calculated respectively with lower formula：
The filial generation ovum number that embodiment 1 is obtained is 1401, and the parental generation ovum number of input is 338, and its " narrow sense " input is produced
Go out rate and be about 414.50%.
The filial generation ovum number that comparative example 1 is obtained is 994, and the parental generation ovum number of input is 356, its " narrow sense " input and output
Rate is about 279.21%, less than embodiment 1.
Comparative example 2
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with the wheat without carbonization treatment
Seed is used the combination control of " cereal kernel --- fresh beanpod " to raise humidity in container and raises east as main spacer medium
Sub- minute pirate bugs.Raised according to the Orius method for breeding described in embodiment 1.According to the east described in embodiment 1
Sub- minute pirate bugs method for breeding is raised.
Embodiment 1 is compared with comparative example 2, it is found that both feeding effect differences are not obvious.But in the east of comparative example 2
The collection phase of sub- minute pirate bugs ovum, has substantial amounts of gelechiid adult, has greatly interfered with the raising operation of Orius.Reason is
The gelechiid larva brill moth that part gelechiid ova in comparative example 2 is hatched without carbonization treatment wheat seed and finally bud into
Adult.And in embodiment 1, also have part gelechiid ova to hatch gelechiid larva, but because of wheat seed Jing carbonization treatment mistakes, therefore gelechiid
Larva can not find the food of abundance, finally because of hungry death, or be taken food by Orius.
Comparative example 3
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, with the wheat seed of Jing carbonization treatments
Grain is used the combination control of " cereal kernel --- fresh beanpod " to raise humidity in container and raises East Asia as main spacer medium
Minute pirate bugs.Kidney bean beanpod is using being front left intact.Carry out according to the Orius method for breeding described in embodiment 1
Raise.Raised according to the Orius method for breeding described in embodiment 1.
Embodiment 1 is compared with comparative example 3, it is found that embodiment 1 is good compared with the feeding effect of comparative example 3.
" narrow sense " input and output rate of embodiment 1 and comparative example 1 is calculated respectively with lower formula：
The filial generation ovum number that embodiment 1 is obtained is 1401, and the parental generation ovum number of input is 338, and its " narrow sense " input is produced
Go out rate and be about 414.50%.
The filial generation ovum number that comparative example 1 is obtained is 877, and the parental generation ovum number of input is 320, its " narrow sense " input and output
Rate is about 274.06%, less than embodiment 1.
This method kidney bean beanpod using it is front through hydrogen peroxide dipping process, on the one hand can kill on beanpod to East Asia
Minute pirate bugs raise unfavorable microorganism (such as entomopathogen) and other insect worm's ovums (such as other kind minute pirate bugs etc.)；It is another
Aspect, hydrogen peroxide (hydrogen peroxide) are strong oxidizer, can aoxidize the agricultural chemicals remained on kidney bean beanpod (non-violated agricultural chemicals), make
Lose toxicity, reduce the impact to Orius as far as possible.
Although an embodiment of the present invention has been shown and described and comparative example, one of ordinary skill in the art can manage
Solution：Without departing from the present invention principle and objective in the case of these embodiments can be carried out various changes, modification, replacement and
Modification, the scope of the present invention are limited by claim and its equivalent.
Claims (9)
1. a kind of method for raising Orius, it is characterised in that using gelechiid ova as main feed, using kidney bean beanpod as
Main oviposition substrate, using the cereal kernel of Jing carbonization treatments as main spacer medium, with " cereal kernel --- fresh beanpod "
Combination control raise humidity in container and raise Orius, key step is as follows：
（1）The hatching of Orius ovum
During the cereal kernel that Jing carbonization treatments are crossed is placed in closing but ventilative raising tank, the kidney bean with Orius ovum is taken
Beanpod is placed on cereal kernel, is hatched；
（2）Orius nymph and the raising of adult
Once it was found that there is Orius nymph to hatch, fresh gelechiid ova is uniformly fed to raising in tank, and fed every 1-2d
Once fresh gelechiid ova；Such as find that the kidney bean beanpod raised in tank is wilted withered and yellow or rots to go mouldy, that is, change fresh kidney bean beanpod,
And make the kidney bean beanpod number in raising tank remain at 8-10 roots；
（3）The collection of Orius ovum
Once it was found that the Orius for raising 8-12% in tank turn into adult, all kidney bean beanpods are taken out every 1-3d, while
Fresh kidney bean beanpod is changed, the kidney bean beanpod number raised in tank remains at 8-10 roots；Kidney bean with Orius ovum
Beanpod can be directly used for breeding the next generation, or low-temperature storage.
2. method according to claim 1, it is characterised in that the step（1）Middle environment of hatching temperature is 25-28 DEG C,
Humidity is 60-70%, and illumination condition is 16L:8D；The step（2）Feeding environment temperature is 25-28 DEG C, and humidity is 60-70%,
Illumination condition is 16L:8D；The step（3）Egg-laying environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:
8D。
3. method according to claim 1, it is characterised in that the control methods of humidity are in the raising tank：By Jing carbon
The cereal kernel that change was processed is placed in glass and raises pot bottom, keeps the fresh kidney bean beanpod number in raising tank all the time in 8-10
Root, controls to raise humidity in tank using the combination of this " cereal kernel --- fresh beanpod ".
4. method according to claim 1, it is characterised in that the raising tank tool is using being front both needed to go out through xeothermic
Bacterium, sterilising conditions are：90 DEG C of temperature, time 10h.
5. method according to claim 1, it is characterised in that the condition of the cereal kernel carbonization treatment is：Temperature 200
DEG C, time 5h；After cereal kernel carbonization treatment, blowing 5h need to be spread in room temperature, clean environment out, the cereal kernel can be little
Wheat seed, barley grain, corn kernel or soya bean seed.
6. method according to claim 1, it is characterised in that the initial feeding volume of the gelechiid ova is 0.5-1.0g, with
Orius age gradually increases, and single gelechiid ova feeding volume also progressively increases to 1.0-2.0g therewith.
7. method according to claim 1, it is characterised in that the kidney bean beanpod be it is fresh undamaged, and never
The agricultural chemicals that the Ministry of Agriculture of China prohibits the use of was contacted, using front, need to be soaked in the hydrogen peroxide solution that mass-volume concentration is 3%
After bubble 30min, room temperature is dried.
8. method according to claim 1, it is characterised in that the step（3）The middle kidney bean beanpod by taking-up is in dissection
Orius ovum is checked whether there is under mirror and is counted, inspection method is under Orius ovum anatomical lens：Orius are normal
Ovum is originated in kidney bean beanpod edge tissues, the marginal swell of the color that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have what is be raised slightly
Circular ovum operculum.
9. method according to claim 1, it is characterised in that the temporary transient condition of storage of the Orius ovum low temperature is：
Temperature 2-5 DEG C, humidity 90%, but storage time are no more than 100d.
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| CN105248382A (en) * | 2015-11-01 | 2016-01-20 | 贵州大学 | Breeding method and device of orius similis |
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| CN106172253B (en) * | 2016-08-01 | 2019-03-29 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
| CN106538480A (en) * | 2016-11-09 | 2017-03-29 | 贵州大学 | A kind of minute pirate bugs release device and method |
| CN106900656B (en) * | 2017-02-06 | 2019-07-30 | 北京市植物保护站 | A kind of cigarette fleahopper industrial production method |
| CN109329221B (en) * | 2018-12-05 | 2021-07-30 | 山东省农业科学院植物保护研究所 | Large-scale breeding method of orius sauteri |
| CN111066732B (en) * | 2019-12-13 | 2021-12-28 | 河南省农业科学院烟草研究所 | Method for breeding orius sauteri |
| CN112997972B (en) * | 2021-03-30 | 2022-09-23 | 中国烟草总公司河南省公司 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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