CN109566541A - A kind of preparation method and application of Similis oviposition medium - Google Patents
A kind of preparation method and application of Similis oviposition medium Download PDFInfo
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- CN109566541A CN109566541A CN201811543308.5A CN201811543308A CN109566541A CN 109566541 A CN109566541 A CN 109566541A CN 201811543308 A CN201811543308 A CN 201811543308A CN 109566541 A CN109566541 A CN 109566541A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
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- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 24
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 24
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Classifications
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- A01K67/033—
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of preparation method and applications of Similis oviposition medium, belong to technical field of insect feeding, the preparation method comprises the following steps: 1) crosscutting whole kidney bean beanpod obtains beanpod tip；2) melt solid paraffin, obtain liquid paraffin；3) notch of step 1) the beanpod tip is immersed into the step 2) liquid paraffin, is sealed, it is cooling, obtain Similis oviposition medium；There is no time sequencing restriction between the step 1) and step 2).The Similis oviposition medium that the present invention is prepared is of good preservation effect, and will not adversely affect to worm's ovum quality and egg hatch rate, it is often more important that, it can be improved the egg laying amount of Similis using Similis oviposition medium of the invention.
Description
Technical field
The present invention relates to technical field of insect feeding more particularly to a kind of Similis oviposition medium preparation method and
Using.
Background technique
Similis Orius similis (zheng) is the sociales in south China area, and prey ability is strong, to Ji
Horse, aphid, tetranychid, aleyrodid, leafhopper, lepidoptera pest ovum and newly hatched larvae have apparent control action, are before one kind is applied
The wide predator of scape.Similis happiness ovum is originated in the fresh and tender site tissue of plant, the plants such as melon, beans it is new
The oviposition substrate of adult when the materials such as bud, tender leaf, petal can be used as its raising.
Currently, the existing largely research in terms of the selection of minute pirate bugs oviposition substrate both at home and abroad.For example, Murai (Murai
T,Narai Y,SugiuraN.2001.Utilization of germinated broad bean seeds as an
oviposition substrate in mass rearing of the predatory bug,Orius sauteri
(Poppius) (Heteroptera:Anthocoridae) .Appl Entomol Zool 36:489-495.) pass through selectivity
It is more suitable for Orius (O.sauteri) oviposition, Lundgren and Fergen with non-selective test discovery beanstalk stalk
(Lundgren JG,FergenJK.2006.The oviposition behavior of the predator Orius
insidiosus:acceptability and preference for different plants.BioControl 51:
217-227.) production of the America minute pirate bugs (O.insidiosus) to plants such as pole bean, soybean, Amaranthus retroflexus, foxtails is tested
Ovum selectivity, discovery pole bean is its most suitable oviposition plant；Guo Jianying and great incomparably (Guo Jianying .2001. mono- great incomparably
Kind it is suitable for breeding oviposition plant-longlive flower kalanchoe Chinese biological prevention and treatment of Orius, 17 (2): 53-56.) to test East Asia small
Flower stinkbug finds that longlive flower kalanchoe is its preferable oviposition substrate to oviposition selectivities such as yellow bean sprout, longlive flower kalanchoes, Zhang Shichang etc. (Zhang Shichang,
Zhou Xingmiao, Wang little Ping, Lei Chaoliang Similis know the oviposition selectivity of host plant and its preservation condition insect of ovum
Know .2008,600-603.) the research discovery Similis matrix that compares hobby oviposition has the tender stem of capsicum and winter jasmine, and it is preceding
Person's brilliant idea ovum amount, low survival rate, and the latter it is low ovum amount, high viability.Moreover, Zhou Xingmiao etc. applies winter jasmine spray
In minute pirate bugs room on mass rearing (application number: CN201510397419.X).Zheng Li etc. then proposes a kind of raising Orius
Method, wherein being using kidney bean beanpod as main oviposition substrate (application number: 201620176955.7).
It is found by numerous studies, kidney bean beanpod is also particularly suitable for Similis oviposition, not only on drawing materials and is fresh-keeping
It is advantageous, and the egg hatching rate of Similis can be a kind of rare oviposition medium plant up to 80% or more, but yet
There are several respects disadvantages: 1) beanpod size (length about 20cm, width about 2cm) is big compared with other oviposition medium plants, limited sky
Between in it is few for the material of Similis oviposition, can virtually be lost because beanpod is overlapped mutually if excessive placement beanpod
For the position of Similis oviposition；2) band ovum beanpod the later period breed polypide during, also due to its size and
Cause to be easy to kill nymph during stirring；3) in refrigeration, also due to the size of beanpod and cause space store on
Inconvenience, if equally will affect the packaging and transport in later period using ovum as product.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation method and applications of Similis oviposition medium, utilize the present invention
Oviposition medium can significantly improve the egg laying amount of Similis.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of preparation methods of Similis oviposition medium, comprising the following steps:
1) crosscutting whole kidney bean beanpod obtains beanpod tip；
2) melt solid paraffin, obtain liquid paraffin；
3) notch of step 1) the beanpod tip is immersed into the step 2) liquid paraffin, is sealed, it is cooling, it obtains
Similis oviposition medium；
There is no time sequencing restriction between the step 1) and step 2).
It preferably, further include being cleaned, being dried to whole kidney bean beanpod before the step 1).
Preferably, position crosscutting described in step 1) is at beanpod tip 1/5~1/3.
Preferably, angle crosscutting described in step 1) is 30~60 °.
Preferably, the notch of beanpod tip described in step 3) immerses the time of liquid paraffin for 0.5~1.5s.
Preferably, the temperature of the step 3) cooling is 20~25 DEG C.
Preferably, the time that the step 2) solid paraffin melts is 80~90s；The temperature that the solid paraffin melts is
70~90 DEG C.
The present invention provides the Similis oviposition media that preparation method described in above scheme is prepared.
The present invention provides application of the oviposition medium of Similis described in above scheme in Similis oviposition, steps
Suddenly are as follows: the oviposition medium of Similis described in above scheme is laid in open containers, is put into the southern little Hua of female, male pairing
Stinkbug, gauze sealing, is cultivated, and Similis worm's ovum is obtained.
Preferably, the temperature of the culture is 25~30 DEG C；The humidity of the culture is 60%~80%.
Beneficial effects of the present invention: the present invention provides a kind of Similis oviposition medium preparation method and application,
The preparation method comprises the following steps: 1) crosscutting whole kidney bean beanpod, obtains beanpod tip；2) melt solid paraffin, obtain
Liquid paraffin；3) notch of step 1) the beanpod tip is immersed into the step 2) liquid paraffin, is sealed, it is cooling, it obtains
To Similis oviposition medium；There is no time sequencing restriction between the step 1) and step 2).What the present invention was prepared
Similis oviposition medium is of good preservation effect, and will not adversely affect to worm's ovum quality and egg hatch rate, more important
, can be improved the egg laying amount of Similis using Similis oviposition medium of the invention.By verification experimental verification, originally
Oviposition medium of Similis the oviposition medium and whole beanpod of invention respectively as Similis, the former day egg laying amount
Acquisition efficiency be 1.5 times of the latter.
Detailed description of the invention
Fig. 1 is the position distribution map of whole kidney bean beanpod, and wherein M is indicated in the middle part of beanpod；T indicates beanpod tip；H indicates beans
Pod head；S indicates beanpod pod face；G indicates beanpod pod seam；
Fig. 2 is kidney bean beanpod tip figure；
Fig. 3 is Similis oviposition medium.
Specific embodiment
The present invention provides a kind of preparation methods of Similis oviposition medium, comprising the following steps:
1) crosscutting whole kidney bean beanpod obtains beanpod tip；
2) melt solid paraffin, obtain liquid paraffin；
3) notch of step 1) the beanpod tip is immersed into the step 2) liquid paraffin, is sealed, it is cooling, it obtains
Similis oviposition medium；
There is no time sequencing restriction between the step 1) and step 2).
In the present invention, the position distribution of the whole kidney bean beanpod is as shown in Figure 1, wherein M is indicated in the middle part of beanpod；T is indicated
Beanpod tip；H indicates beanpod head；S indicates beanpod pod face；G indicates beanpod pod seam.
The present invention preferably further includes being cleaned, being air-dried to whole kidney bean beanpod before crosscutting whole kidney bean beanpod；
The kidney bean beanpod is preferably that surface is not damaged and the sharp complete beanpod of beans；The kidney bean beanpod is from commercially available；The present invention couple
The length and width of the kidney bean beanpod is not particularly limited.
In the present invention, the mode of the cleaning is preferably first rinsed, rear to rinse；The reagent of the rinsing is preferably dish washing liquid
Water；The volume ratio of dish washing liquid and water is preferably 0.1~0.2:1500~2500 in the dish washing liquid water, and more preferably 0.15:
2000；The present invention does not have particular/special requirement to the brand of the dish washing liquid；The time of the rinsing is preferably 20~40s, more preferably
For 30s；The reagent of the flushing is preferably water；The time of the flushing is preferably 10~25s, more preferably 15~20s.This hair
In bright, the effect of the cleaning is to wash off beanpod chemical agent remained on surface.
In the present invention, the temperature of the drying is preferably 20~30 DEG C, and more preferably 25~28 DEG C；The time of the drying
Preferably 20~40min, more preferably 25~35min, most preferably 30min；The mode of the drying preferably air-dries, more excellent
It is selected as cleaned beanpod being laid on filter paper, water suction paper handkerchief wipes beanpod surface water droplet, carries out air-dried；Described air-dried sets
Standby preferably electric fan, more preferably desk fan；The power of the electric fan is preferably 30~50w, more preferably 40w；The electricity
The quantity of fan is preferably 2；The position of the electric fan is preferably oppositely arranged in beanpod two sides；The blowing of the electric fan
Position is preferably beanpod gap.In the present invention, the effect of the drying is to ensure that beanpod gap position is dry and comfortable.
In specific implementation process of the invention, the electric fan be preferably purchased from beauty mechanical desk fan (300mm, 40
Watt)；In the process of processing, the maximum gear of preferably beautiful electric fan is blowed.
The present invention is after cleaning, being dried to whole kidney bean beanpod, crosscutting whole kidney bean beanpod, obtains beanpod point
Portion；The crosscutting position is preferably at beanpod tip 1/5~1/3, more preferably at beanpod tip 1/4；At this
In the specific implementation process of invention, the crosscutting position is located at beans point profile and curves the position for directly depending on inner 2~3cm, is detailed in Fig. 2
The position that arrow is directed toward in the figure of kidney bean beanpod tip；The crosscutting angle is preferably 30~60 °, and more preferably 40~50 °, most
Preferably 45 °；The crosscutting tool is preferably scalpel, more preferably by the scalpel of disinfection treatment.
In the present invention, the transversal position is preferably that the purpose at beanpod tip 1/5~1/3 is to obtain beanpod point
Portion lays eggs conducive to Similis.
It is that Similis hobby is produced in kidney bean beanpod seam and kidney bean beanpod tip that the present invention, which selects the reason of beanpod tip,
Ovum, the ovum amount of kidney bean beanpod tip account for whole kidney bean beanpod 50% or more of ovum amount.Therefore, kidney bean beanpod tip is prepared into
The oviposition medium of Similis can increase Similis oviposition medium in the confined space compared with whole kidney bean beanpod
Quantity, while being also able to achieve the efficient utilization of kidney bean beanpod.
In the present invention, the crosscutting angle be preferably 30~60 ° of effect be in sealing step after being conducive to uniformly
Waxing and tight sealing.
In the present invention, melt solid paraffin, obtain liquid paraffin；The time that the solid paraffin melts is preferably 80~
90s, more preferably 85s；The temperature that the solid paraffin melts is preferably 70~90 DEG C, and more preferably 80 DEG C；The thawing
Solid paraffin is preferably put in water by mode, carries out heating water bath thawing, more preferably heating water bath melts under agitation
Change；The water is preferably distilled water；The ratio of the area of the quality and water surface of the solid paraffin be preferably 4~10g:90~
120cm2, more preferably 5~8g:100~110cm2.In specific implementation process of the invention, the device for melting solid paraffin
Ware is preferably beaker, more preferably glass beaker；The specification of the beaker is preferably 1L；The bore of the beaker is preferably
12cm；The solid paraffin is preferably solid paraffin particle；The partial size of the particle is preferably 3~4mm；The solid paraffin
Gross mass is preferably 4~6g, more preferably 5g.
In the present invention, the ratio that the quality of solid paraffin and the area of the water surface is arranged is 4~10g:90~120cm2, favorably
Notch in beanpod tip is uniformly waxed and tight seal, while can be avoided paraffin and excessively causing to waste and paraffin is avoided to invade
Account for too many beanpod gap.
The present invention immerses the liquid stone after obtaining beanpod tip and liquid paraffin, by the notch of the beanpod tip
Wax is sealed, cooling, obtains Similis oviposition medium；The notch of the beanpod tip immerses the time of liquid paraffin
Preferably 0.5~1.5s, more preferably 1s；The immersion angle of the beanpod tip is preferably dipped vertically into；The temperature of the cooling
Preferably 20~25 DEG C, more preferably 22~24 DEG C of degree；The mode of the cooling preferably immerses cooling in distilled water.
In the present invention, the purpose of the sealing is package beanpod notch, prevents the infection of beanpod tip and water loss, plays
The effect fresh-keeping to beanpod tip.
The present invention provides the Similis oviposition media that preparation method described in above scheme is prepared；The south
Minute pirate bugs oviposition medium is as shown in Figure 3.
The present invention provides application of the oviposition medium of Similis described in above scheme in Similis oviposition, steps
Suddenly are as follows: the oviposition medium of Similis described in above scheme is laid in open containers, is put into the southern little Hua of female, male pairing
Stinkbug, gauze sealing, is cultivated, and Similis worm's ovum is obtained；The temperature of the culture is preferably 25~30 DEG C, more preferably
26~28 DEG C, most preferably 27 DEG C；The humidity of the culture is preferably 60%~80%, and more preferably 65%~75%, it is optimal
It is selected as 70%；The culture is preferably preferably 12~16:8~12 for photoperiod (L:D), more preferably 14:10.The present invention couple
The position that is put into of Similis does not have particular/special requirement, and Similis can lay eggs in beanpod tip automatically.
In the present invention, the placed angle of the open containers is preferably open and places obliquely；The angle obliquely
Preferably 40~50 °, more preferably 45 ° of degree；The open containers are preferably transparent glass container；The volume of the glass container
Preferably 4~5dm3, most preferably 4.5dm3；The cross section of the glass container is preferably 2.5~3.5dm2, most preferably
3dm2；The hatch bore diameter of the glass container is preferably 10~15cm, more preferably 12~14cm；The number of plies of the gauze is preferred
It is 1~2 layer；The aperture of the gauze is preferably 60~100 mesh, more preferably 70~80 mesh；The effect of the gauze is to prevent
Similis is escaped from from container.
In specific implementation process of the invention, the open containers are preferably purchased from rich (4.5 cubes of glass of Shandong wound
Decimetre capacity, angle storing glass jar).
Below with reference to embodiment to the preparation method and application of Similis oviposition medium provided by the invention a kind of into
Row detailed description, but they cannot be interpreted as limiting the scope of the present invention.
A kind of preparation method of the Similis of embodiment 1 oviposition medium
1. the cleaning of kidney bean beanpod
It selects that surface is not damaged and the sharp complete kidney bean beanpod of beans, is placed in 2L clear water, the dish washing liquid of 0.15mL is added,
Then it is rinsed, beanpod of holding with a firm grip in water washes away 30s repeatedly up and down, rinses 15s under tap water after lifting beanpod completely.This mistake
Journey is to wash off beanpod chemical agent remained on surface.
2. the kidney bean beanpod after cleaning is dry
Washed beanpod is layered on filter paper one by one, the water droplet on beanpod is gently wiped using water suction paper handkerchief, then in beans
Pod two sides are blowed using the electric fan of beauty, and beanpod gap position need to be aligned to the direction of wind, to ensure beanpod gap
Position is dry and comfortable, blow-time 30min, and temperature is 28 DEG C.
3. cutting beanpod tip
Prepare the scalpel disinfected, at 1/4 close to beanpod tip, using the oblique 45 ° of crosscutting beanpods of scalpel,
Obtain beanpod tip.
4. notch is waxed
700mL distilled water is added in 1000mL glass beaker, weighs 5g granular solids wax, and be put into beaker；Then whole
A beaker is put into digital display thermostat water bath, and temperature is set as 75 DEG C, when temperature is close to set temperature, glass bar can be used to exist
It is stirred clockwise in beaker (60rpm), observes paraffin in beaker and melt (90s) completely.It takes the beans nose part cut,
Cut out portion downward, then quickly take out, and bean or pea disengaging liquid level process is maintained at by wax layer that is vertical, rapidly entering floating on water
1s is completed, and then notch the same manner of first-class wax is put into cold distilled water (24 DEG C) again, enables first-class wax quick
Condensation, tight notch obtain Similis oviposition medium.
Embodiment 2 verifies the fresh-keeping situation of the Similis oviposition medium of embodiment 1
By the above-mentioned Similis oviposition medium being prepared and beanpod tip, the whole beanpod of equivalent amount, set
At 27 ± 1 DEG C of temperature, the environmental condition of 70 ± 5% humidity, observation Similis oviposition medium and whole beanpod it is fresh-keeping
Situation, 10 repetitions of each processing.Comparing result is referring to table 1, as the result is shown: lay eggs in embodiment 1 medium and whole kidney bean beans
The fresh-keeping situation of pod is substantially similar.
1 Similis of table lay eggs medium, beanpod tip and whole beanpod fresh-keeping situation
Influence of the Similis oviposition medium of 3 embodiment 1 of embodiment to egg hatch rate
The Similis worm that Similis oviposition medium and whole beanpod to embodiment 1 are obtained as oviposition medium
Ovum is hatched respectively, is observed hatching rate (27 ± 1 DEG C of temperature, 70 ± 5% humidity), 15 repetitions of each processing.As a result referring to
Table 2, as the result is shown: the Similis oviposition medium and whole beanpod of embodiment 1 as oviposition medium, incubating by obtained worm's ovum
Change time upper no significant difference, ovum is substantially completely hatched within 7 days, and hatching rate is up to 80%.It can be seen that Similis oviposition is situated between
Matter can't have an adverse effect to the quality of Similis worm's ovum, will not influence the hatching of worm's ovum.
The hatching rate (%) of the different incubation medium Similis ovum under different brooding times of table 2
The egg laying amount verifying of the Similis oviposition medium of 4 embodiment 1 of embodiment
15 normed spaces are set up, are equally divided into 3 groups, Similis oviposition media group, whole a small amount of group of beanpod, whole
Beanpod volume group, normed space are glass container, and the specification of glass container is 4.5dm3Space, 3dm2Cross section.South is small
The Similis oviposition medium of 10 embodiments 1 that tile respectively in flower stinkbug oviposition media group glass container；Whole beanpod is a small amount of
Tile 3 whole beanpods respectively in group glass container；Tile 6 whole beanpods respectively in whole beanpod volume group glass container,
Beanpod is laid with more crowded；Each glass container accesses the Similis of 5 pairs of female, male pairings respectively, and environment temperature is arranged
It is 27 DEG C, humidity 70%, photoperiod 14:10 (L:D) then counts day egg laying amount.For experimental result referring to table 3, experimental result is aobvious
Show, is organized using Similis oviposition than whole beanpod of media group of embodiment 1 and be collected into whole beanpod volume group on a small quantity
The quantity of worm's ovum is all more.
The day egg laying amount of Similis under the conditions of 3 different egg of table
As seen from the above embodiment, the present invention provides a kind of Similis oviposition medium preparation method and application,
The Similis oviposition medium can significantly improve the collection efficiency of Similis ovum compared with whole beanpod.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of Similis oviposition medium, comprising the following steps:
1) crosscutting whole kidney bean beanpod obtains beanpod tip；
2) melt solid paraffin, obtain liquid paraffin；
3) notch of step 1) the beanpod tip is immersed into the step 2) liquid paraffin, is sealed, it is cooling, obtain south
Minute pirate bugs oviposition medium；
There is no time sequencing restriction between the step 1) and step 2).
2. preparation method according to claim 1, which is characterized in that further include to whole rue before the step 1)
Beans beanpod is cleaned, is dried.
3. preparation method according to claim 1 or 2, which is characterized in that crosscutting position described in step 1) is close
At beanpod tip 1/5~1/3.
4. preparation method according to claim 3, which is characterized in that crosscutting angle described in step 1) is 30~60 °.
5. preparation method according to claim 1, which is characterized in that the notch of beanpod tip described in step 3) immerses liquid
The time of state paraffin is 0.5~1.5s.
6. preparation method according to claim 1 or 5, which is characterized in that the temperature of the step 3) cooling is 20~25
℃。
7. preparation method according to claim 1, which is characterized in that the time that the step 2) solid paraffin melts is 80
~90s；The temperature that the solid paraffin melts is 70~90 DEG C.
The medium 8. Similis that preparation method described in claim 1~7 any one is prepared is laid eggs.
Application of the medium in Similis oviposition, step are as follows: right 9. Similis described in claim 8 is laid eggs
It asks the 8 Similis oviposition media to be laid in open containers, is put into the Similis of female, male pairing, gauze seals,
It is cultivated, obtains Similis worm's ovum.
10. application according to claim 9, which is characterized in that the temperature of the culture is 25~30 DEG C；The culture
Humidity is 60%~80%.
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