CN110463666B - Indoor propagation method for orius minutus - Google Patents
Indoor propagation method for orius minutus Download PDFInfo
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- CN110463666B CN110463666B CN201910857206.9A CN201910857206A CN110463666B CN 110463666 B CN110463666 B CN 110463666B CN 201910857206 A CN201910857206 A CN 201910857206A CN 110463666 B CN110463666 B CN 110463666B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The invention provides an indoor propagation method of orius pollidipennis, and relates to the technical field of insect feeding. The method comprises the steps of egg collection, egg hatching and nymph breeding, adult collection and egg refrigeration. According to the method, the host plant for breeding the thrips and the oviposition carrier of the lygus lucorum are set as the kidney bean pods, so that the breeding steps are simplified, and the development of scale breeding of the lygus lucorum is facilitated. The padding arranged in the oviposition device can reduce the humidity by about 10 percent so as to improve the survival rate of adults and the number of eggs laid; the hatchability can be improved by 20% by matching covers with different hollow diameters during hatching; the sucked adults are quickly mated in a culture dish in advance before being placed in a spawning device, and the spawning number of the single-root kidney beans can be increased by 105%. By utilizing the method, the obtained eggs of the orius minutus are refrigerated for 8 days at the temperature of 8 ℃, the hatching rate of the eggs can be ensured to be more than 70%, and the adult collection rate is about 30%.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to an indoor propagation method for orius pollidipennis.
Background
The orius miniatus (oriussstrigilis) has the characteristics of wide predatory property, strong adaptability and the like, has obvious control effect on thrips, aphids, spider mites, whiteflies, leafhoppers, lepidoptera pest eggs and newly hatched larvae, and is a predatory natural enemy with wide application prospect. The problem of indoor propagation of the orius pollidii is solved, and the key link for field utilization is realized.
At present, there are reports of indoor mass propagation technology and method for Orius miniatus at home and abroad, for example, Ito adopts eggs of Mediterranean pink borer as food, soybean seedlings as spawning substrate to propagate Orius miniatus indoors in large quantities (ItoK. A simple mass-reproducing method for reproducing Orius bugs in the laboratory, applied energy and Zoology,2007,42(4):573 577); bueno et al established a large-scale indoor feeding system for southern Orius minutus (Bueno V, Mendes SM, Carvalho LM. evaluation of a obtaining-method for the receptor organ antigens. Bullein of the science 2006,59(1):1-6) by selecting a glass jar and a corrugated cardboard as feeding environmental conditions for the southern Orius minutus, and also taking eggs of the mediterranean borer as food and flowers of Bidens pilosa as an egg laying substrate and a water source; zhang Chang Rong et al combined Frankliniella occidentalis and Aphis fabae to feed orius nasutus, tender stem of Ach sinensis as oviposition medium (patent No. CN105325377B), Zhongxing Miao et al also uses tender branch of Ach sinensis as oviposition medium, food is artificial feed (application No. CN201510397419.X) prepared independently, Zhengli et al proposed a method for feeding orius nasutus, i.e. using wheat moth eggs (capable of industrial production) as main feed, and kidney bean pod as main oviposition medium (application No. 201620176955.7).
At present, under the condition that the artificial feed for the orius minutus is not mature, the most important parts for large-scale feeding are food and oviposition substrates, the food is basically an insect living body, and the oviposition substrates are plant tissues. It is not inconceivable that if the insect, which is the food of the orius pollidii, feeds on the host plant which is just also a suitable oviposition substrate for the orius pollidii, the process can be simplified and the costs can be reduced. For example, if the southern orius bug, the sticktight or the winter jasmine are fed with frankliniella occidentalis or aphis fabae as a spawning medium, the preparation is carried out by 1) feeding insects as food, and preparing a host plant such as a legume plant from which such insects feed in advance; 2) and (3) cultivating winter jasmine serving as a spawning substrate. At least 2-3 plants and 3 insects are prepared in the links, and when one link falls down, the large-scale feeding of the orius pollidii can be stopped.
Disclosure of Invention
In view of the above, the invention aims to provide an indoor propagation method for orius minutus, which obviously simplifies the breeding process and saves the cost, and the hatchability of the refrigerated orius minutus is more than 70%, and the adult collection rate is about 30%.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an indoor propagation method for orius minutus, which comprises the following steps: (1) in a stinkbug spawning device with a first padding and filter paper sequentially paved from bottom to top, a spawning substrate and a kidney bean pod carrying a food source are paved on the filter paper, adult stinkbugs are put in, and a cover is covered for cultivation; the first padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite, 42-48 parts of wood shaving sawdust and 36-45 parts of silica gel desiccant; the egg-laying substrate is cleaned kidney bean pods; each kidney bean pod carrying the food source carries 180-220 of thrips nymphs;
(2) collecting kidney bean pods carrying the eggs of the orius minutus on the next day, and supplementing fresh kidney bean pods and food sources with the same quantity as the collected kidney bean pods;
(3) placing the kidney bean pods carrying the orius minutus eggs in a hatching device, hatching and culturing nymphs, and taking out the adults and placing the adults in a culture dish for mating every day when the adults appear;
(4) and placing the mated adult orius minutus into the orius minutus oviposition device, collecting the orius minutus eggs, and refrigerating and storing the orius minutus eggs.
Preferably, the orius minutus spawning device in the step (1) comprises a circular inclined-opening glass storage tank, the length of the storage tank is 16cm, the width of the storage tank is 22cm, the height of the storage tank is 23cm, the caliber of the inclined opening is 12cm, the diameter of the tank cover is 13cm, and the volume of the tank cover is 4.3L; the stinkbug spawning device is subjected to high-temperature sterilization treatment before use; the middle hollow part of the cover is circular with the diameter of 9cm, and the hollow part is adhered to the cover through a 100-mesh gauze without gaps.
Preferably, the number ratio of the oviposition matrix to the kidney bean pods carrying food sources in the step (1) is 3: 1; the female-male quantity ratio of the adult orius minutus is 2: 1.
Preferably, the temperature of the culture in the step (1) is 27 ℃, the humidity is 70%, and the light irradiation: the dark time was 14h:10 h.
Preferably, the collecting method in the step (2) includes clamping the kidney beans by using tweezers, and shaking off the thrips and the adult orius minutus on the kidney beans.
Preferably, the hatching device in the step (3) is the same as the stinkbug laying device, and a second pad and filter paper are laid on the bottom of the hatching device; the second padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite and 42-48 parts of wood shavings.
Preferably, during the incubation and nymph culture process in step (3), different covers are used according to different stages of incubation: the hollow part in the middle of the cover used in the hatching beginning 3d is in a circular shape with the diameter of 5cm, and the hollow part is adhered to the cover by a 100-mesh gauze without gaps; and 4d, replacing the hollow part in the middle with a round shape with the diameter of 9cm, and adhering the hollow part with a 100-mesh gauze without gaps.
Preferably, in the hatching and nymph culturing process, 1 kidney bean pod carrying 180-220 thrips nymphs is added every day from the 4 th day of hatching; and starting from 8d, adding 2 kidney bean pods carrying 180-220 thrips nymphs every day.
Preferably, the number of the insects in the culture dish in the step (3) is not more than 15, and the placing time is within 2 h.
Preferably, the temperature for cold storage in the step (4) is 4-8 ℃, and the time for cold storage is 5-8 days.
The invention provides an indoor propagation method of orius pollidii, which comprises the steps of egg collection, egg hatching and nymph breeding, adult collection and egg refrigeration. According to the method, the breeding steps of the thrips as the feed are simplified, and meanwhile, breeding and spawning carriers of the thrips and the lygus minutus are set as kidney bean pods, so that the development of scale breeding of the lygus minutus is facilitated:
(1) according to the invention, the padding materials are flatly laid in the spawning device, the matching and combination of the padding materials are more scientific and reasonable, and the temperature and humidity, especially the humidity, are effectively controlled;
(2) the hatching device is matched with covers with different hollow diameters during hatching, so that the hatching rate and the survival rate can be improved;
(3) in the incubation and nymph culture stage, after adult insects are sucked out, the adult insects are temporarily and rapidly mated in a culture dish and then placed in a spawning device, mating is promoted by using a narrow space of the culture dish, and the efficiency is obviously improved;
(4) when the method is used for indoor propagation of the orius minutus, the obtained orius minutus eggs are refrigerated for 8 days in an environment of 8 ℃, the hatching rate of the eggs can be ensured to be more than 70%, and the adult collection rate is close to 30%.
Drawings
FIG. 1 is a device for the oviposition of orius minutus;
FIG. 2 is a thrips raising pot;
FIG. 3 is a diagram of the preparation stage of egg-laying medium;
FIG. 4 is a rapid mating map of adult orius minutus in a culture dish;
fig. 5 is an egg of orius minutus laid on kidney bean pods.
Detailed Description
The invention provides an indoor propagation method for orius minutus, which comprises the following steps: (1) in a stinkbug spawning device with a first padding and filter paper sequentially paved from bottom to top, a spawning substrate and a kidney bean pod carrying a food source are paved on the filter paper, adult stinkbugs are put in, and a cover is covered for cultivation; the first padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite, 42-48 parts of wood shaving sawdust and 36-45 parts of silica gel desiccant; the egg-laying substrate is cleaned kidney bean pods; each kidney bean pod carrying the food source carries 180-220 of thrips nymphs;
(2) collecting kidney bean pods carrying the eggs of the orius minutus on the next day, and supplementing fresh kidney bean pods and food sources with the same quantity as the collected kidney bean pods;
(3) placing the kidney bean pods carrying the orius minutus eggs in a hatching device, hatching and culturing nymphs, and taking out the adults and placing the adults in a culture dish for mating every day when the adults appear;
(4) and placing the mated adult orius minutus into the orius minutus oviposition device, collecting the orius minutus eggs, and refrigerating and storing the orius minutus eggs.
In the expanded propagation method, in a stinkbug spawning device with a first padding and filter paper sequentially paved at the bottom, a spawning substrate and a kidney bean pod carrying a food source are paved on the filter paper, adult stinkbugs are put in, and a cover is covered on the adult stinkbugs for culturing; the first padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite, 42-48 parts of wood shaving sawdust and 36-45 parts of silica gel desiccant; the egg-laying substrate is cleaned kidney bean pods; and each kidney bean pod in the kidney bean pods carrying the food source carries 180-220 thrips nymphs. The orius minutus oviposition device is preferably a circular inclined-opening glass storage tank as shown in figure 1, the length of the storage tank is 16cm, the width of the storage tank is 22cm, the height of the storage tank is 23cm, the caliber of the inclined opening is 12cm, the diameter of the tank cover is 13cm, and the volume of the tank cover is 4.3L. The orius minutus oviposition device is preferably subjected to high-temperature sterilization treatment before use, and the high-temperature sterilization treatment is preferably carried out for 2 hours at 105 ℃. The filter paper of the present invention preferably has a size of 20X 20 cm. The stinkbug spawning device further comprises a cover, the middle hollow part of the cover is preferably circular with the diameter of 9cm, and the hollow part is adhered to the stinkbug through a 100-mesh gauze without gaps. The raw material of the first padding of the present invention preferably includes: 345-355 parts of vermiculite, 43-46 parts of wood shavings and 38-42 parts of silica gel desiccant, and more preferably comprises 350 parts of vermiculite, 45 parts of wood shavings and 40 parts of silica gel desiccant. The vermiculite is preferably large-particle vermiculite with the particle size of 1-3 mm. The padding is more scientific and reasonable in matching and combination, can effectively control the temperature and the humidity, and particularly can reduce the humidity by about 10 percent, so that the situation that water vapor is not formed in a spawning device and the situation that adult insects are submerged or kidney beans are too wet to interfere the selection of spawning positions of the adult insects is avoided
The egg-laying substrate is cleaned dry kidney bean pods, and the preparation method of the egg-laying substrate takes 30 bean pods as an example, and preferably comprises the following steps: placing 30 pods in 2L of clear water, adding 0.15mL of detergent, rinsing, holding the pods tightly in the water, repeatedly washing for 30s up and down, and washing for 15s in tap water after completely lifting the pods; the washed pods were spread one by one on filter paper, and water drops on the pods were gently wiped off with absorbent paper towels and then blown on both sides of the pods. The air blowing is preferably performed by using an American electric fan, the pod gap position needs to be aligned to the direction of the incoming air so as to ensure that the pod gap position is dry and comfortable, the air blowing time is 30min, and the temperature is 28 ℃. The power of the electric fan is preferably 30-50 w, and more preferably 40 w. The cleaning method can be used for cleaning the chemical agents remained on the surfaces of the bean pods.
The food source is preferably kidney bean pods eaten by thrips for more than 5 days, and the preparation method of the food source preferably comprises the following steps: setting a thrips breeding pot with a pot mouth diameter of 26cm and a volume of 4L; placing large-particle vermiculite with the weight of 110g in the basin, and flatly paving a piece of qualitative filter paper (25 x 25cm) on the vermiculite; the breeding pot cover is made of circular organic glass with the thickness of 0.5cm and the diameter of 33cm, a circular hole with the diameter of 22cm is hollowed in the middle of the pot cover, and the hollowed-out part is adhered to the pot cover through a 100-mesh gauze without a gap. Each rearing pot is filled with 8 fresh and washed kidney bean pods, about 200 young thrips and 100 adult thrips, the number of thrips is observed daily and 2 fresh and washed kidney beans are supplemented, the yellow and rotten kidney beans in the rearing pot of thrips are cleaned daily and put into a thrips and orius minutus retriever, preferably as shown in chinese patent CN 201821795513.6.
The number ratio of the oviposition substrate to the kidney bean pods carrying food sources is preferably 3:1, namely 3 oviposition substrates are flatly laid on filter paper of the oviposition device, and a food source (kidney bean pods) carrying a certain number of thrips is placed above the oviposition substrates. The amount of the adult orius minutus put in the culture device is preferably 15 heads/egg laying device, the female-male ratio of the adult orius minutus is preferably 2:1, and then the adult orius minutus is quickly covered with a cover for culture. The temperature of the culture is preferably 27 ℃, the humidity is 70%, and the light irradiation: the dark time ratio was 14h to 10 h. In the invention, 3 spawning substrates can be exactly covered with filter paper, so that the filter paper is not overcrowded, and the spawning efficiency is favorably maximized; and about 200 thrips are on each kidney bean pod, which is the daily eating amount of 15 lygus bugs, so that the food deficiency of the lygus bugs is avoided, and the waste of the thrips is avoided.
The kidney bean pods with the small stink bugs eggs are collected on the next day, and the same quantity of fresh kidney bean pods and food sources as the collected kidney bean pods are supplemented. The collecting method preferably comprises the steps of clamping kidney beans by using tweezers, shaking off thrips and adult orius bugs on the kidney beans, and further brushing off the kidney beans by using a brush pen if the number of the kidney beans is large. The kidney beans are required to be supplemented with the same amount of fresh kidney beans after being collected, and the same amount of thrips is required to be added every day as supplement. The collecting of the invention preferably further comprises cleaning the dead body of the orius minutus every day by using an insect suction pipe, and abandoning the spawning tank and resetting the tank when the adult orius minutus in the spawning tank is less than 5.
According to the method, after kidney bean pods carrying tiny flower bug eggs are collected, the kidney bean pods carrying tiny flower bug eggs are placed in a hatching device for hatching and nymph cultivation, and after adults appear, the adults are taken out by a bug sucker every day and placed in a culture dish for mating. The hatching device is preferably the same as the stinkbug spawning device, and a second pad and filter paper are paved at the bottom of the hatching device; the second padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite and 42-48 parts of wood shavings. In the hatching and nymph culturing processes, different covers are preferably used according to different stages of hatching so as to improve the hatching rate and the survival rate: the hollow part in the middle of the cover used in the hatching beginning 3d is in a circular shape with the diameter of 5cm, and the hollow part is adhered by a gauze with 100 meshes without gaps (the hollow diameter is small, so that the humidity in a cylinder can be improved, and the hatching of eggs can be facilitated); and 4d, starting from the beginning, replacing the hollow part in the middle with a round shape with the diameter of 9cm, and adhering the hollow part by a gauze with 100 meshes without gaps (the humidity in the cylinder needs to be reduced, and water drops are easily caused by overhigh humidity). In the hatching and nymph culturing process, 1 kidney bean pod carrying 200 nymphs is added every day preferably from the 4 th day of hatching; starting at 8d, 2 kidney bean pods carrying 200 thrips nymphs were added daily. In the hatching and nymph culturing process, the kidney beans which are dried and mildewed in the hatching tank are preferably cleaned every day and put into a thrips and lygus lucorum recoverer, and the thrips and lygus lucorum recoverer is preferably shown as Chinese patent CN 201821795513.6. The adult bugs are temporarily placed in a culture dish, the number of the adult bugs placed in the culture dish is preferably not more than 15, the placing time is within 2h, and the small space of the culture dish is utilized to promote the adult bugs to mate in advance before the adult bugs are placed in a spawning tank. The raw material of the second mat material of the present invention preferably includes: 345 to 355 parts of vermiculite and 43 to 46 parts of wood shavings, and more preferably 350 parts of vermiculite and 45 parts of wood shavings. The vermiculite is preferably large-particle vermiculite with the particle size of 1-3 mm.
Placing the mated adult orius minutus into the orius minutus oviposition device, collecting the orius minutus eggs, and refrigerating and storing the orius minutus eggs. According to the method for collecting the stinkbug eggs, 10 kidney bean pods are taken as an example, preferably, the method comprises the steps of putting 10 kidney beans carrying the stinkbug eggs into a low-density polyethylene fresh-keeping bag (25 x 30cm), and ensuring that bean tips of the pods are outward; then the freshness protection package is put into a plastic box (25 x 35cm), and the plastic box is placed in a refrigerator for storage. The temperature of the refrigeration preservation is preferably 4-8 ℃, and more preferably 8 ℃. The time for refrigerating and storing is preferably 5-8 d, and more preferably 8 d.
The present invention provides a method for indoor propagation of orius pollidii with reference to the following examples, which should not be construed as limiting the scope of the present invention.
Example 1
1. Thrips breeding
Setting a thrips breeding pot (as shown in figure 2): the diameter of the basin mouth is 26cm, and the volume is 4L; placing large-particle vermiculite with the weight of 110g in the basin, and flatly paving a piece of qualitative filter paper (25 x 25cm) on the vermiculite; the breeding pot cover is made of circular organic glass with the thickness of 0.5cm and the diameter of 33cm, a circular hole with the diameter of 22cm is hollowed in the middle of the pot cover, and the hollowed-out part is adhered to the pot cover through a 100-mesh gauze without a gap. Each rearing pot was filled with 8 fresh and washed kidney bean pods, with approximately 200 young thrips and 100 adult thrips, and the number of thrips was observed daily and supplemented with 2 fresh and washed kidney beans. The yellow and rotten kidney beans in the thrips breeding pot are cleaned every day and put into a thrips and orius minutus recoverer.
2. Collection of eggs
1) An spawning tank (shown in figure 1) is provided: the spawning tank is a round inclined-opening glass storage tank, the length of the tank is 16cm, the width of the tank is 22cm, the height of the tank is 23cm, the diameter of the inclined opening is cm, the diameter of the tank cover is cm, and the volume of the tank cover is 4.3L; laying packing materials at the bottom of a spawning tank, wherein the packing materials comprise large-particle vermiculite (1-3 mm, weight of 350g), wood shavings and wood chips (weight of 45g) and two bags of 20g of silica gel desiccant (produced by Kunshan Weisheng desiccant Co., Ltd.), and high-temperature sterilization treatment (drying at 105 ℃ for 2 hours) is needed before use; qualitative filter paper (20 x 20cm) was laid flat over the pad.
2) Preparation of oviposition matrix (as shown in fig. 3): the spawning substrate is a fresh and unbroken kidney bean pod which is sold in the market, and the length and the width of the spawning substrate are not particularly limited. Placing 30 pods in 2L of clear water, adding 0.15mL of detergent, rinsing, holding the pods tightly in the water, repeatedly washing for 30s, and washing for 15s under tap water after completely lifting the pods. The washed pods are paved on filter paper one by one, water drops on the pods are lightly wiped by using a water absorption paper towel, then electric fans (the power of the electric fans is preferably 30-50 w and more preferably 40w) are used on two sides of the pods for blowing, and the pod gap position needs to be aligned to the wind direction to ensure that the pod gap position is dry and comfortable, the blowing time is 30min, and the temperature is 28 ℃.
3) Setting spawning: 3 kidney beans are flatly laid above the filter paper of each spawning tank, and 1 kidney bean carrying 200 thrips nymphs is put in the spawning tank (the kidney bean is guaranteed to be eaten by the thrips for more than 5 days); blowing 15 adult orius miniatus into each spawning tank by using an insect suction pipe, wherein the female-male ratio is 2:1, and then quickly covering a modified tank cover, namely a circular hollow part with the middle diameter of 9cm, wherein the hollow part is adhered by a gauze with 100 meshes without gaps. The temperature of the culture was 27 ℃, the humidity was 70%, and the photoperiod (L: D) was 14: 10.
4) And (4) collecting eggs. Collecting the kidney beans carrying the stinkbug eggs every other day by clamping the kidney beans by using tweezers and shaking off the thrips and the adults of the stinkbug on the kidney beans, wherein the kidney beans can be further brushed away by using a writing brush if the number of the adults is large. After collection, the kidney beans are supplemented with the same amount of fresh kidney beans, and the same amount of thrips is added every day as supplement. And (3) cleaning the dead body of the orius minutus every day by using an insect suction pipe, and abandoning the spawning tank and resetting the tank when the adult orius minutus in the spawning tank is less than 5.
3. Incubation of eggs and nymph rearing
And arranging a tiny orius bugus hatching tank, and basically hatching eggs in 3d after delivery. The hatching tank and the spawning tank are the same glass tanks, but no silica gel drying agent is placed in the hatching tank, and the tank covers used at different time stages have different styles. 8 kidney beans carrying the eggs of the orius similis are placed in each hatching tank, a tank cover is used in 3d and is in a circular shape with the middle hollow part being 5cm in diameter, and the hollow part is stuck by a 100-mesh gauze without gaps. Beginning at the 4 th step, changing the shape into a round shape with the middle hollow diameter of 9cm, adhering the hollow part with a gauze of 100 meshes without gaps, and adding 1 kidney bean carrying 200 nymphs of thrips; starting at 8d, 2 kidney beans carrying 200 thrips nymphs were added. The dried and mildewed kidney beans in the hatching tank are cleaned every day and are placed into a thrips and orius minutus recoverer.
4. Adult collection
Observing the occurrence condition of the adult orius miniatus in the hatching tank every day, and if the adult orius miniatus is found, immediately sucking the adult orius miniatus out by using an insect sucking pipe for standby. The sucked adult orius minutus is temporarily placed in a plastic culture dish (the diameter is 9cm, as shown in figure 4), the number of the adult is controlled within 15 heads (the number of male insects is not lower than 5 heads), and the placing time is not more than 2 h. The adult small orius bugs in the plastic culture dish are transferred to a newly-arranged spawning tank according to the specified quantity, and a spawning link is performed, and spawning is shown in fig. 5.
5. Egg refrigeration
The egg has the strongest cold resistance and is the best insect state in cold storage. And part of the orius pollidipennis eggs are refrigerated to reasonably control the indoor population quantity of the orius pollidipennis and are supplemented when needed, so that the risk of 'goods failure' in the process of the propagation of the orius pollidipennis can be well avoided. Putting 10 kidney beans carrying the eggs of the orius minutus into a low-density polyethylene fresh-keeping bag (25 x 30cm), and ensuring that the bean tips of the pods face outwards; then the freshness protection package is put into a plastic box (25 x 35cm), and the plastic box is placed in a refrigerator for storage. The refrigeration condition and the refrigeration period of the orius pollidii ova are respectively 8 ℃ and 8 days, the hatchability of the ova can be ensured to be more than 70%, and the adult collection rate is close to 30%.
In the implementation process of example 1, tests such as the effect of silica gel desiccant on the temperature and humidity in the jar (50 times of repetition), the effect of different types of jar covers on the hatching of the orius minutus eggs (5 times of repetition), the development duration of the eggs and nymphs in the hatching jar (15 times of repetition), and the hatching rate of the eggs under different refrigeration conditions (5d) and the adult collection rate (20d) (15 times of repetition) were also performed, and the results are shown in tables 1 to 5:
TABLE 1 influence of silica gel desiccant on temperature and humidity in cylinder
| Treatment of | Average temperature (. degree. C.) | Average humidity (% RH) |
| Empty cylinder | 27.5 | 76.9 |
| Vermiculite and wood dust | 29.0 | 75.7 |
| Vermiculite, wood dust and 1 pack drying agent | 27.9 | 78.9 |
| Vermiculite, wood dust and 2-pack drying agent | 27.9 | 68.3 |
| Vermiculite, wood dust and 3-pack drying agent | 28.0 | 67.2 |
TABLE 2 Effect of different types of pot lids on hatching of orius minutus eggs
| Treatment of | Average hatching rate of eggs (%) |
| Hollow diameter of 5cm | 92.6 |
| Hollow diameter of 9cm | 72.5 |
TABLE 3 incubation in-vat development of eggs and nymphs
| Insect status | Average calendar period (d) |
| Egg | 2.8 |
| 1 st nymph | 2.5 |
| 2-year-old nymphs | 1.7 |
| 3-year-old nymphs | 1.7 |
| 4-year-old nymphs | 1.5 |
| Nymphs of 5 years old | 2.9 |
| Undeveloped stage (Total) | 13.1 |
TABLE 4 incubation Rate of eggs under different refrigeration conditions (5d) and adult Collection Rate (20d)
TABLE 5 Effect of Rapid mating of Ornitus parvifolius adults on egg production
Note: the ratio of the male and female insects is 2:1 (15 heads in total), the kidney bean pods are 3, and other feeding conditions are consistent.
The invention provides an indoor propagation method for orius minutus, which simplifies the feeding link and reduces the cost, and the obtained orius minutus eggs are refrigerated for 8 days at the temperature of 8 ℃, so that the hatchability of the eggs can be ensured to be more than 70%, and the adult collection rate is nearly 30%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. The indoor propagation method for orius pollidii includes the following steps: (1) in a stinkbug spawning device with a first padding and filter paper sequentially paved from bottom to top, a spawning substrate and a kidney bean pod carrying a food source are paved on the filter paper, adult stinkbugs are put in, and a cover is covered for spawning culture; the first padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite, 42-48 parts of wood shaving sawdust and 36-45 parts of silica gel desiccant; the egg-laying substrate is cleaned kidney bean pods; each kidney bean pod carrying the food source carries 180-220 of thrips nymphs; the orius minutus spawning device comprises a circular inclined-opening glass storage tank, the length of the storage tank is 16cm, the width of the storage tank is 22cm, the height of the storage tank is 23cm, the caliber of the inclined opening is 12cm, the diameter of a tank cover is 13cm, and the volume of the tank cover is 4.3L; the stinkbug spawning device is subjected to high-temperature sterilization treatment before use; the middle hollow part of the cover is circular with the diameter of 9cm, and the hollow part is adhered to the cover through a 100-mesh gauze without a gap; the number ratio of the oviposition matrix to the kidney bean pods carrying food sources is 3: 1; the female-male quantity ratio of the adult orius minutus is 2: 1;
(2) collecting kidney bean pods carrying the eggs of the orius minutus on the next day, and supplementing fresh kidney bean pods and food sources with the same quantity as the collected kidney bean pods;
(3) placing the kidney bean pods carrying the orius minutus eggs in a hatching device, hatching and culturing nymphs, and taking out the adults and placing the adults in a culture dish for mating every day when the adults appear; the hatching device is the same as the stinkbug spawning device, and a second padding and filter paper are paved at the bottom of the hatching device; the second padding comprises the following raw materials in parts by weight: 340-360 parts of vermiculite and 42-48 parts of wood shavings and sawdust; during the incubation and nymph culture process, different lids are used, depending on the different stages of incubation: the hollow part in the middle of the cover used in the hatching beginning 3d is in a circular shape with the diameter of 5cm, and the hollow part is adhered to the cover by a 100-mesh gauze without gaps; beginning at the 4d, the middle hollow part is changed into a round shape with the diameter of 9cm, and the hollow part is adhered to the gauze with 100 meshes without gaps; in the hatching and nymph culturing processes, 1 kidney bean pod carrying 180-220 thrips nymphs is added every day from the 4 th day of hatching; adding 2 kidney bean pods carrying 180-220 thrips nymphs every day from 8 d;
(4) and placing the mated adult orius minutus into the orius minutus oviposition device, collecting the orius minutus eggs, and refrigerating and storing the orius minutus eggs.
2. The method according to claim 1, wherein the temperature of the culture in the step (1) is 27 ℃, the humidity is 70%, and the ratio of light: the dark time was 14h:10 h.
3. The method according to claim 1, wherein the collecting of step (2) comprises picking the kidney beans with tweezers and shaking off the adults of the thrips and the small bugs on the kidney beans.
4. The method of claim 1, wherein the number of the insects placed in the culture dish in the step (3) is not more than 15, and the placing time is within 2 h.
5. The method of claim 1, wherein the temperature of the refrigerated storage in the step (4) is 4-8 ℃, and the time of the refrigerated storage is 5-8 days.
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