WO2014010314A1 - Method for cultivating mushrooms using reusable fiber substrate, and culture medium for cultivation using same - Google Patents

Abstract

The purpose of the present invention is to provide a technique for cultivating mushrooms that can considerably reduce waste material produced after cultivation, and that can recover and reuse a culture substrate after cultivation. Mushroom cultivation is made possible when a culture medium for cultivation is used, the culture medium for cultivation being impregnated with water and a nutrient source using a reusable fiber substrate as a culture substrate. Furthermore, the culture substrate can be easily recovered after cultivation, and the culture medium can be washed in a simple manner using an existing washing device and reused as a culture substrate, and is capable of considerably reducing waste material produced by mushroom cultivation.

Description

Straw cultivation method using reusable fiber as a base material, and cultivation medium used therefor

The present invention relates to a method for cultivating straw by fungus bed cultivation. More specifically, the present invention relates to a method for growing straw that can reuse the culture substrate and contribute to the reduction of waste. Furthermore, this invention relates to the culture medium used for the said cultivation method.

In recent years, salmon has been attracting attention as a health food in response to growing health consciousness. Conventionally, many commercially available cocoons have been produced by artificial fungus bed cultivation using a cultivation medium in which water and nutrients are added to sawdust. However, the amount of sawdust, which becomes waste after cultivation, has increased dramatically due to the spread of fungus bed cultivation and the increase in production of straw. For this reason, there are concerns about the waste disposal method by disposable sawdust after use, and there is a concern about the future supply shortage of sawdust.

Therefore, conventionally, attempts have been made to reduce the amount of sawdust used in the cultivation medium for strawberries. For example, Patent Document 1 proposes a method for cultivating straw by using a cultivation medium in which waste paper material is obtained by crushing waste paper material into small pieces and mixing sawdust. However, the method of Patent Document 1 is not limited to the technology that enables the reduction of sawdust, and enables the cultivation of potato beds without using sawdust. Furthermore, in the method of Patent Document 1, it is still difficult to reuse the culture medium after cultivation, and it is difficult to say that it contributes to effective utilization of resources.

In addition, some technologies that enable fungus bed cultivation without using sawdust have been reported. For example, Patent Document 2 discloses that by using a spherical molded product such as glass beads and ceramic balls as a culture substrate, the culture substrate can be recovered and reused even after cultivation. Furthermore, Patent Document 3 discloses that the culture substrate can be reused by using a granular culture substrate made of a synthetic resin material in the cultivation medium for strawberries. However, in the methods of Patent Documents 2 and 3, in addition to the culture substrate, it is necessary to use a relatively large amount of a water retention material such as vermiculite and a water retention insoluble nutrient source such as rice bran and bran. However, it is difficult to separate the culture substrate from the cultured medium, and there is a problem that an apparatus for cleaning the culture substrate must be developed. Furthermore, the water-retaining material and the water-retaining insoluble nutrient source become waste after cultivation, and thus have a drawback in terms of reducing waste.

JP 2003-310050 A JP 2003-9656 A JP 2003-134933 A

An object of the present invention is to provide a cultivation technique for straw that can easily collect and reuse a culture substrate after cultivation, and can greatly reduce waste generated after cultivation.

The present invention has been intensively studied to solve the above problems. As a result, when a reusable fiber base material is used as a culture base material, and a culture medium impregnated with water and nutrient sources is used, It became possible to grow. In addition, the culture medium can easily recover the culture base material after cultivation, can be easily washed using an existing cleaning device, and can be reused as a culture base material. It has also been found that can be greatly reduced. The present invention has been completed by further studies based on these findings.

That is, this invention provides the cultivation method of the grape of the aspect hung up below, and the culture medium for cultivation of straw.
Item 1. A method for cultivating persimmons, comprising cultivating persimmon beds using a reusable fiber base material, water, and a culture medium containing a nutrient source.
Item 2. Item 2. The cultivation method for strawberries according to Item 1, wherein the reusable fiber substrate is a linear fiber substrate or a sheet fiber substrate.
Item 3. Item 3. A method for cultivating a koji according to item 1 or 2, wherein the fungus bed cultivation of koji is performed by bottle cultivation or bag cultivation.
Item 4. Item 4. The method for cultivating cocoons according to any one of Items 1 to 3, wherein a fiber base material is collected from the cultivation medium after cultivating cocoons, and the cultivating cocoon is repeated by reusing the fiber base material as a culture substrate.
Item 5. Item 5. The method for cultivating koji according to any one of Items 1 to 4, wherein the koji is at least one selected from the group consisting of oyster mushrooms, enokitake mushrooms, eringgi, tamogitake mushrooms, beech shimeji mushrooms, shiitake mushrooms and maitake mushrooms.
Item 6. Item 6. The cultivation of strawberries according to any one of items 1 to 5, wherein the strawberries are at least one selected from the group consisting of enokitake, oyster mushrooms, eringi, tamogitake, and bunashimeji, and the fungus bed cultivation is performed in bottle cultivation. Method.
Item 7. Item 6. The method for cultivating a cocoon according to any one of Items 1 to 5, wherein the cocoon is at least one selected from the group consisting of shiitake mushroom, bamboo shoot, oyster mushroom, and maitake.
Item 8. A culture medium for straw cultivation, comprising a reusable fiber substrate, water, and a nutrient source.
Item 9. Item 10. The cultivation medium for strawberries according to Item 8, wherein the reusable fiber substrate is a linear fiber substrate or a sheet fiber substrate.
Item 10. Item 10. The cultivation medium for strawberries according to Item 8 or 9, which is a medium for bottle cultivation.
Item 11. Item 10. A medium for cultivation of strawberries according to Item 8 or 9, which is a medium for bag cultivation.
Item 12. Item 12. The culture medium for cultivating persimmons according to any one of Items 8 to 11, which is used for cultivating at least one kind of persimmon selected from the group consisting of oyster mushrooms, enokitake mushrooms, eringi, tamogitake, bunashimeji, shiitake mushrooms and maitake.
Item 13. Item 11. The medium for cultivation of koji according to item 10, which is used for cultivation of at least one kind of koji selected from the group consisting of enokitake, oyster mushrooms, eringi, tamogitake, and bunashimeji.
Item 14. Item 12. The cultivation medium for strawberries according to Item 11, which is used for cultivation of at least one kind of strawberries selected from the group consisting of shiitake mushrooms, bamboo shoots, oyster mushrooms, and maitakes.

According to the present invention, the fungus bed can be cultivated without using sawdust, so that the problem of waste due to disposable sawdust and the future sawdust supply problem can be solved. In addition, according to the present invention, the culture substrate can be easily recovered from the cultivation medium after cultivation, easily washed with an existing washing apparatus such as a washing machine, and reused as the culture substrate. Things can be greatly reduced. Furthermore, in the present invention, the culture substrate used as the culture substrate itself has water retention, so that the fungus bed can be cultivated without using a water retention material. It can also be a basic technology for realizing hydroponics.

In addition, in the conventional fungus bed cultivation of straw, it is necessary to select the kind of sawdust according to the kind of straw to be cultivated, and the cultivation medium had to change the kind of sawdust depending on the kind of straw. However, according to the present invention, since the cultivation medium can be prepared without using sawdust, there is also an advantage that the cultivation medium can be easily prepared.

Furthermore, in the conventional fungus bed cultivation, the cultivation bottle filled with the culture medium becomes heavy, and much labor is required for transporting the bottle. In contrast, in the present invention, the weight can be reduced to about 25% compared to sawdust medium, and the size of the cultivation bottle itself may be reduced depending on the culture solution. is there. Moreover, when using a sawdust culture medium, the facility which stores the sawdust which is the raw material is needed, but since a sawdust is not used in this invention, a storage place can be eliminated. And, in normal sawdust medium, long time high temperature and high pressure sterilization is required for sterilization, but in the present invention, the sterilization time can be greatly shortened. As described above, the present invention can simplify the technique of fungus bed cultivation and rationalize the field, and has a great commercial advantage compared to conventional fungus bed cultivation.

In FIG. 1, the result of having observed the external appearance of the enokitake mushroom after cultivation in Example 1 is shown. When gauze is used as a fiber base material for A (condition 1), when a towel is used as a fiber base material (condition 2), and when a super absorbent towel is used as a fiber base material for C (conditions) 3) and D show the results when felt is used as the fiber substrate (condition 3). In FIG. 2, the result of having observed the external appearance of the enokitake mushroom after cultivation in Example 1 is shown. When A linen is used as a fiber base for A (Condition 5), When cotton cloth is used as a fiber base for B (Condition 6), When linen string is used as a fiber base for C (Conditions) 7) and D show the results when one hand towel is used as the fiber base (condition 8), and E shows the results when three hand towels are used as the fiber base (condition 9). In FIG. 3, the result of having observed the appearance of the oyster mushroom after cultivation grown in Example 2 using three wet towels (condition 11) as a fiber base material is shown. In FIG. 4, the result of having observed the external appearance of the eringi after cultivation grown in Example 3 using one hand towel (condition 9) as a fiber base material is shown. FIG. 5 shows the result of observing the appearance of beech shimeji grown using one hand towel (condition 5) as the fiber base in Example 5. In FIG. 6, the result of observing the appearance of the bamboo leaf grown by bag cultivation using three towels as the fiber base material in Example 6 is shown. In FIG. 7, the result of observing the appearance of the oyster mushroom cultivated by bag cultivation using three hand towels as the fiber base material in Example 7 is shown. FIG. 8 shows the result of observing the appearance of shiitake mushrooms grown by bag cultivation using four wet towels as the fiber base material in Example 8.

The cultivation method for strawberries according to the present invention is characterized in that the straw is cultivated using a cultivation medium containing a fiber base material, water and a nutrient source. Hereinafter, the present invention will be described in detail.

There are no particular limitations on the type of koji that can be used in the present invention to be cultivated , and examples include oyster mushrooms, enokitake mushrooms, eringgi, tamogitake mushrooms, beech shimeji, nameko, stake mushrooms, shiitake mushrooms, and maitake. Among these, oyster mushrooms, shiitake mushrooms, enokitake mushrooms, eringgi, tamogitake mushrooms, beech shimeji mushrooms, shiitake mushrooms, and maitake mushrooms are suitable as application target pods of the cultivation method of the present invention. Moreover, the cultivation method of this invention can be applied not only to a conventionally well-known cocoon but also to the cocoon newly discovered or produced in the future.

Cultivation Medium In the present invention, a cultivation medium containing a reusable fiber substrate, water, and a nutrient source is used. Thus, by using a fiber base material as a culture base material for a culture medium, fungal bed culture of strawberries is realized without using sawdust.

In the present invention, the reusable fiber base material is a linear or sheet base material formed from fibers, and even if it is used once as a culture base material for straw cultivation medium, It can be washed and reused in an industrial washing machine or a commercial washing machine. Specific examples of such fiber base materials include linear fiber base materials such as yarns, ropes, strings, and ropes; and sheet-like fiber base materials such as nonwoven fabrics, woven fabrics, knitted fabrics, and felts. Among these, sheet-like fiber substrates such as nonwoven fabrics, woven fabrics, knitted fabrics, and felts are preferably used in the present invention because they can be easily collected and washed after cultivation.

∙ The fibers constituting the reusable fiber base material are not particularly limited, and may be natural fibers or chemical fibers. Specific examples of natural fibers that can constitute the fiber substrate include cotton, hemp, wool, silk, and cashmere. Specific examples of chemical fibers that can constitute the fiber substrate include polyesters such as polyethylene terephthalate and polybutylene terephthalate, polyamides such as nylon 6 and nylon 6,6, acrylics such as polyacrylonitrile, polyolefins such as polyethylene and polypropylene, Synthetic fibers such as polyurethane; regenerated fibers such as rayon, cupra and polynosic; semisynthetic fibers such as acetate and promix. These fibers may be used individually by 1 type, and may be used in combination of 2 or more type.

Since the fiber base material also plays a role as a water retention material in the culture medium, it is desirable to use a material having a high water retention capacity in the present invention.

The culture medium used in the present invention contains water. It does not restrict | limit especially as water used for preparation of the culture medium for cultivation, For example, any of tap water, ion-exchange water, distilled water, ultrapure water, etc. may be sufficient.

About the content of the water in the culture medium for cultivation, it sets suitably according to the kind, quantity, etc. of the fiber base material to be used. For example, it may be set as appropriate according to the type of the fiber substrate within a range where water is about 120 to 360 g per 100 g of the fiber substrate.

Further, the culture medium used in the present invention includes a nutrient source required for growing straw. Examples of such nutrient sources include insoluble nutrient sources such as rice bran, bran, corn bran, okara, brewer's yeast, soybean meal, etc .; water extracts of these insoluble nutrient sources, sucrose, casamino acids, inorganic salts, thiamine hydrochloride And other soluble nutrient sources. These nutrient sources may be used alone or in combination of two or more.

For the water extract of the insoluble nutrient source among the soluble nutrient sources, for example, about 3 to 10 ml of water per 1 g of the solid nutrient source is mixed and heated as necessary (for example, about 10 to 80 at about 130 to 130 ° C.). It can be obtained by heating for ~ 30 minutes), extracting, and collecting the liquid fraction.

What is necessary is just to set suitably about the content of the nutrient source in the culture medium for cultivation according to the kind of the cultivation target, the kind of nutrient source to be used, and the like. In this invention, since the collection | recovery for reuse of a culture | cultivation base material becomes easy after cultivation, so that the usage-amount of an insoluble nutrient source is reduced, it is preferable that the usage-amount of an insoluble nutrient source is low. When reducing the amount of insoluble nutrient source to be used, the nutrient source required for the growth of the cocoon can be supplemented by using an aqueous extract of the insoluble nutrient source. In the cultivation medium to be used, the addition ratio of the soluble nutrient source and the insoluble nutrient source may be appropriately set in consideration of the type of each nutrient source to be used and the degree of growth of mycelia.

Cultivation method The cultivation method of the koji of the present invention is carried out by cultivating the mushroom bed using the cultivation medium. As a method for cultivating the fungus bed of koji, any of bottle cultivation, bag cultivation, box cultivation and the like may be used, and preferably bottle cultivation and bag cultivation are mentioned. In this invention, except using the said culture medium, the conditions of fungal bed cultivation, such as bottle cultivation, bag cultivation, and box cultivation, are the same as that of normal fungal bed cultivation.

Hereinafter, the method for carrying out the cultivation method for straw of the present invention will be described by taking bottle cultivation and bag cultivation as examples.

[Bin cultivation]
Bacteria bed cultivation by bottle cultivation is carried out through steps such as bottle stuffing, sterilization, inoculation, culture, and if necessary, bacteria scraping, budding, growth, and harvesting.

“Bottled” is a step of filling the culture medium in the culture bottle. In the bottle filling, the culture medium prepared in advance may be packed in a culture bottle, or the mixture of water and nutrients may be added to the culture bottle after the fiber base material is accommodated in the culture bottle. The amount of the culture medium accommodated in the culture bottle is appropriately set according to the size of the culture bottle. Usually, the culture medium is packed so that it occupies 80 to 95% of the volume of the culture bottle. That's fine.

“Sterilization” is a process of killing microorganisms in culture bottles or culture media. Sterilization is usually performed by a heat sterilization method. Specific conditions for sterilization include 100 to 130 ° C. for 10 to 30 minutes.

“Inoculation” is a process of planting inoculum in a culture medium that is allowed to cool after sterilization. As the inoculum, either a liquid inoculum obtained by culturing in a liquid medium or a solid inoculum obtained by culturing on an agar medium may be used. The inoculation amount of the inoculum is the same as in the case of general bottle cultivation.

“Cultivation” is a process of spreading and maturing mycelium. The culture conditions are appropriately set according to the type of koji used, the size of the culture bottle, and the like, and usually include about 8 to 36 days at 25 ° C. in the dark. More specifically, at 25 ° C. in the dark, about 8 to 36 days for oyster mushrooms, about 13 to 36 days for enokitake mushrooms, about 10 to 31 days for eringi, about 10 to 31 days for mushrooms 9 to 16 days, and about 14 to 33 days for Bunashimeji. Note that the relative humidity during culture is approximately 100% because the bottle lid is closed.

“Bacteria scraping” is a process performed as necessary, and is a process of scraping off the inoculum part and the culture medium surface.

“Sprouting” is a process of forming and growing fruit body primordia and young fruit body. The conditions for germination are appropriately set according to the type of koji used, and usually, about 3 to 30 days under conditions of 10 to 1000 lux and 15 to 18 ° C. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., oyster mushroom is about 7 to 15 days, enokitake is about 12 to 19 days, eringi is about 11 to 18 days, About 3 to 11 days, and about 18 to 30 days for Bunashimeji. The relative humidity at the time of sprouting is almost 100% because it is performed with the bottle lid closed.

“Growth” is a process of growing a fruit body primordium or a fruit body into a mature fruit body that can be harvested. Moreover, you may adjust the moisture content in a culture medium by pouring water into the culture medium as needed after scraping a fungus or during germination. The growth conditions are appropriately set according to the type of koji to be used, the size of the culture bottle, etc., and are usually about 6 to 16 days under conditions of 10 to 1000 lux and 15 to 18 ° C. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., oyster mushrooms are about 6 to 8 days, enoki mushrooms are about 9 to 11 days, eringi are about 9 to 11 days, About 6 to 8 days, and about 12 to 16 days for Bunashimeji. The relative humidity during growth is almost 100% because the bottle lid is closed.

By performing the above steps, a mature fruit body of cocoon can be obtained. When the mature fruit body of the persimmon is harvested, the whole process of the persimmon bottle cultivation is completed.

茸 Examples of koji suitable for bottle cultivation include enokitake mushrooms, oyster mushrooms, eringi, tamogitake and bunashimeji.

[Bag cultivation]
Bacteria bed cultivation by bag cultivation is carried out through various processes such as bagging, sterilization, inoculation, culture, budding, growth, and harvesting.

“Bag filling” is a process of filling the culture medium in a culture bag. The bag filling may be performed by impregnating the fiber base material with a mixture of water and nutrient sources and then filling the culture bag. Also good. Further, the amount of the culture medium packed in the culture bag can be appropriately set according to the size of the culture bag or the fiber base material, but is usually about 42 to 71% with respect to the volume of the culture bag. Adjust it.

“Sterilization” and “inoculation” are as described in the bottle cultivation. In addition, the inoculation amount of the inoculum in the “inoculation” step is the same as in the case of general bag cultivation.

“Culture” refers to the same process as in the case of bottle cultivation. The culture conditions for bag cultivation are also appropriately set according to the type of koji used, the size of the culture bottle, and the like, and are usually about 13 to 90 days at 25 ° C. in the dark. More specifically, under conditions of 25 ° C. and dark, about 17 to 20 days for Tamogitake, about 13 to 18 days for Oyster mushrooms, and about 90 days for Shiitake mushrooms. The relative humidity during the culture is almost 100% because the bag is closed.

“Sprouting” refers to the same process as in the case of bottle cultivation. The conditions for budding in the case of bag cultivation are also set as appropriate according to the type of koji used, and are usually about 3 to 10 days under conditions of 10 to 1000 lux and 15 to 18 ° C. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., about 3 to 7 days for Tamotake, about 6 to 10 days for oyster mushroom, and about 5 to 7 days for shiitake It is done. In general, sprouting is performed by removing the culture medium from the bag and exposing the surface, and after sprinkling the culture medium, it is performed in an environment where the relative humidity is 80 to 100%. Moreover, you may adjust the moisture content in a culture medium by sprinkling water to the culture medium for cultivation as needed.

“Growth” refers to the same process as in the case of bottle cultivation. The growth conditions in the case of bag cultivation are also set as appropriate according to the type of koji used, the size of the culture bottle, etc., and are usually about 7 to 10 days under conditions of 10 to 1000 lux and 15 to 18 ° C. Can be mentioned. More specifically, under conditions of 10 to 1000 lux and 15 to 18 ° C., about 7 to 9 days for Tamogitake, about 7 to 10 days for Oyster mushrooms, and about 7 to 9 days for Shiitake mushrooms It is done. The growth is usually carried out with the culture medium removed from the bag and the surface exposed, and in an environment with a relative humidity of 80 to 100%. Moreover, you may adjust the moisture content in a culture medium by sprinkling water to the culture medium for cultivation as needed during growth.

By performing the above steps, a mature fruit body of cocoon can be obtained. When the mature fruit body of the cocoon is harvested, the whole process of bag cultivation of the cocoon is completed.

茸 Types of koji suitable for bag cultivation include shiitake mushrooms, bamboo shoots, oyster mushrooms, and maitake mushrooms. Among them, shiitake mushrooms are suitable as koji for bag cultivation because the hyphal growth direction is not constant.

In any cultivation method, after cultivation of straw, the fiber base material recovered from the used medium and washed can be reused as a culture base material again. The fiber base material can be easily collected after use, and can be easily washed with an existing washing apparatus such as a washing machine.

Hereinafter, the present invention will be specifically described by way of examples. However, the present invention is not limited to the scope of the following examples.

Example 1: Enokitake Bin Cultivation Preparation of culture solution 300 ml of water was added to 50 g of rice bran, sterilized at high temperature and high pressure at 121 ° C. for 10 minutes, allowed to cool, and then the liquid fraction was taken out using gauze to obtain a hot water extract of rice bran.

Separately, a nutritional replenisher (10 ml / L of sucrose, 10 g / L of casamino acid, 5 g / L of Murashige-Skoog mixed salt powder, and 10 μg / L of thiamine hydrochloride (the remainder is water) was prepared.

By mixing 30 mL of the rice bran hot water extract obtained above and 30 mL of the nutrient replenisher, and further adding 0.6 g (final concentration is 1% by weight) of brown sugar to this mixed solution, the culture solution is obtained. Was prepared.

2. Preparation of culture medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1 to 8 shown in Table 1) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 9 shown in Table 1), Each fiber base material shown in Table 1 was folded and packed into the shape shown in Table 1, and 2 g of rice bran was added from above. Next, the amount of the culture solution prepared above was added as shown in Table 1, and a polypropylene screw cap was formed. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per wet towel used in conditions 8 and 9 is 60 to 100 ml.

3. Cultivation of Enokitake A stock of Enokitake was cultured on a petri dish with a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 1), and the mycelium was spread throughout the medium.

Thereafter, the bacteria were scraped and water was poured to such an extent that the surface of the medium was moistened. When culturing for a predetermined period (Table 1) under light conditions of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, when cultured for about 10 days under the same conditions, a mature fruiting body was obtained.

Table 1 summarizes the results of enokitake cultivation. Moreover, the result of having observed the appearance of the enokitake mushroom after cultivation on each condition is shown in FIGS. The results of cultivation using the fiber base material shown in Table 1 are the growth rate, the number of days of cultivation, and the child, when the cultivation medium containing 20 g of sawdust, 16 g of rice bran, and 65 mL of water is packed in a 200 mL culture bottle and grown. It was about the same size in terms of size and shape.

Example 2: Oyster mushroom bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Preparation of culture medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1 to 10 shown in Table 2) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 11 shown in Table 2), Each fiber base material shown in Table 2 was folded and packed into the shape shown in Table 2, and 2 g of rice bran was added from above. Subsequently, the culture solution prepared above was added in the amount shown in Table 2, and a screw cap made of polypropylene was used. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per hand towel used in conditions 10 and 11 is 60 to 100 ml.

3. Cultivation of oyster mushrooms A stock strain of oyster mushrooms cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 2), and the mycelium was spread throughout the medium.

Thereafter, the bacteria were scraped and water was poured to such an extent that the surface of the medium was moistened. When culturing for a predetermined period (Table 2) under a light condition of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.

Table 2 summarizes the results of cultivation of oyster mushrooms under each condition. Moreover, the result of having observed the appearance of the oyster mushroom after cultivation on condition 11 is shown in FIG. The cultivation results using the fiber base material shown in Table 2 are the growth rate, the number of days of cultivation, and the case where the cultivation medium containing sawdust 20 g, rice bran about 16 g and water 65 mL is packed in a 200 mL culture bottle and oyster mushrooms are cultivated. The size and shape of the fruiting bodies were similar.

Example 3: Elingi bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Preparation of culture medium In a 200 mL culture bottle (inner diameter 4.5 cm, conditions 1 to 9 shown in Table 3) or a 500 mL culture bottle (inner diameter 8.0 cm, conditions 10 shown in Table 3), Each fiber base material shown in Table 3 was folded and packed into the shape shown in Table 3, and 2 g of rice bran was added from above. Subsequently, the culture solution prepared above was added in the amount shown in Table 3, and a polypropylene screw cap was formed. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of culture solution added per wet towel used under conditions 9 and 10 is in the range of 60 to 100 ml.

3. Cultivation of eringi A stock strain of eringi was cultured on a petri dish having a diameter of 90 mm containing a potato dextrose agar medium (PDA medium) for 14 days under dark conditions at 25 ° C. as a seed. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time under dark conditions at 25 ° C. (Table 3), and the mycelium was spread throughout the medium.

Thereafter, the bacteria were scraped and water was poured to such an extent that the surface of the medium was moistened. When culturing for a predetermined period (Table 3) under a light condition of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, further culturing for about 10 days under the same conditions yielded a mature fruiting body.

Table 3 summarizes the results of cultivating eringi under each condition. Moreover, the result of having observed the external appearance of the eringi after cultivation on condition 9 is shown in FIG. The results of cultivation using the fiber base material shown in Table 3 are the growth rate, cultivation days, fruiting body, and the case where cultivation medium containing 20 g of sawdust, 16 g of rice bran, and 65 mL of water is packed in a 200 mL culture bottle and cultivated eringi. In terms of size and shape.

Example 4: Cultivation of Tamogitake bottles Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Preparation of culture medium Each fiber substrate shown in Table 4 was folded and packed into a 200 mL culture bottle (inner diameter: 4.5 cm) in the shape shown in Table 4, and 2 g of rice bran was added thereto. Next, the amount of the culture solution prepared above was added as shown in Table 4 to form a screw cap made of polypropylene. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per towel used under condition 10 is 60 to 100 ml.

3. Cultivation of Tamogitake A stock strain of Tamogitake was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days, and was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 4), and the mycelium was spread throughout the medium.

Then, the lid was removed, distilled water was added to the full bottle from the top of the medium, and the watering operation was performed for 2 hours. When cultured for a predetermined period of time under light conditions of 25 ° C. and 300 lux (Table 4), fruiting body primordia were confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.

Table 4 summarizes the results of cultivating Tamogitake under each condition. The cultivation results using the fiber substrate shown in Table 4 are the growth rate, the number of cultivation days, and the child, when cultivation medium containing 20 g of sawdust, 16 g of rice bran and 65 mL of water is packed in a 200 mL culture bottle and grown It was about the same size and shape.

Example 5: Buna shimeji bottle cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Preparation of culture medium Each fiber base material shown in Table 5 was folded into a 200 mL culture bottle (inner diameter: 4.5 cm) and packed in the shape shown in Table 5, and 2 g of rice bran was added thereto. Next, the amount of the culture solution prepared above was added as shown in Table 5, and a screw cap made of polypropylene was used. This was cultivated at 121 ° C. for 15 minutes at high temperature and high pressure to prepare a culture medium. It has been confirmed that the optimal amount of the culture solution added per towel used in condition 5 is 60 to 100 ml.

3. Cultivation of Buna Shimeji A stock of Buna shimeji was cultured on a petri dish having a diameter of 90 mm containing a potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 20 days as a seed fungus. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 10 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 5), and the mycelium was spread throughout the medium.

Thereafter, the bacteria were scraped and water was poured to such an extent that the surface of the medium was moistened. When culturing for a predetermined period (Table 5) under light conditions of 15 ° C. and 300 lux instead of the lid with the membrane, the fruiting body primordium was confirmed. After confirming the formation of the fruiting body primordium, further culturing for about 14 days under the same conditions, a mature fruiting body was obtained.

Table 5 summarizes the results of cultivating Bunashimeji under each condition. Moreover, the result of having observed the appearance of the beech shimeji after cultivation on condition 5 is shown in FIG. The cultivation results using the fiber base materials shown in Table 5 are the growth rate, the number of cultivation days, and the child, when cultivating beech shimeji mushrooms in a 200 ml culture bottle containing 20 g of sawdust, 16 g of rice bran and 65 mL of water. It was about the same size and shape.

Example 6: Bag cultivation of Tamogitake Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Cultivation medium preparation: A 45cm long x 20cm wide cultivation bag with 60-100ml of broth per piece soaked in 27cm x 27cm horizontal towel (cotton) Three sheets were laid on top of each other. Between the wet towels, 10 g of rice bran was placed so as to spread over the whole towel, so that 20 g of rice bran was added in total. As a result, the volume of the fiber base material in the cultivation bag was about 420 cm ³ . This was sterilized at 121 ° C. for 30 minutes at a high temperature and high pressure to prepare a culture medium for cultivation.

3. Cultivation of Tamogitake A stock strain of Tamogitake was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days, and was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 6), and the mycelium was spread throughout the medium.

Thereafter, a hand towel with a spread of hyphae is taken out from the bag, placed in a plastic tapper, sprayed with water by spraying, and then cultured for a predetermined period of time under light conditions of 15 ° C. and 300 lux (Table 6). The group was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.

Example 7: Oyster mushroom bag cultivation Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Cultivation medium preparation: A 45cm long x 20cm wide cultivation bag with 60-100ml of broth per piece soaked in 27cm x 27cm horizontal towel (cotton) Three sheets were laid on top of each other. Between the wet towels, 10 g of rice bran was put so as to spread over the whole wet towel, and 20 g of rice bran was put in total. As a result, the volume of the fiber base material in the cultivation bag was about 420 cm ³ . Then, the culture medium for cultivation was prepared by performing high temperature and high pressure sterilization at 121 degreeC for 30 minutes.

3. Cultivation of oyster mushrooms A stock strain of oyster mushrooms cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days was used as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 7), and the mycelium was spread throughout the medium.

After that, the hand towel in which the mycelium has spread is taken out from the bag, put into a plastic tapper, sprayed with water by spraying, and then cultured at 15 ° C. under a light condition of 300 lux for a predetermined period (Table 7). It was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.

Example 8: Bag cultivation of shiitake mushrooms Preparation of culture solution A culture solution was prepared in the same manner as in Example 1.

2. Preparation of cultivation medium 4 pieces of 27cm long x 27cm wide hand towels soaked with 60-100ml culture medium in a 45cm long x 20cm wide cultivation bag Laid on top of each other. Between the wet towels, 10 g of rice bran was put so as to spread over the whole wet towel, and a total of 30 g of rice bran was put. As a result, the volume of the fiber base material in the cultivation bag was about 560 cm ³ . Then, the culture medium for cultivation was prepared by performing high temperature and high pressure sterilization at 121 degreeC for 30 minutes.

3. Cultivation of shiitake mushrooms A stock strain of shiitake mushrooms was cultured on a petri dish having a diameter of 90 mm containing potato dextrose agar medium (PDA medium) under dark conditions at 25 ° C. for 14 days as an inoculum. The grown mycelium was punched with a cork borer having a diameter of 3 mm, and 30 cells were inoculated on the surface of the culture medium prepared above. Culture was performed for a predetermined period of time at 25 ° C. under dark conditions (Table 8), and the mycelium was spread throughout the medium.

Thereafter, a hand towel in which hyphae have spread is taken out from the bag, placed in a plastic tapper, sprayed with water by spraying, and then cultured under a light condition of 15 ° C. and 300 lux for a predetermined period of time (Table 8). It was confirmed. After confirming the formation of the fruiting body primordium, when further cultured for about 7 days under the same conditions, a mature fruiting body was obtained.

Claims (14)

1. 茸 Cultivation method for culturing persimmons using a culture medium containing reusable fiber base material, water, and nutrient sources.
2. The cultivation method for strawberries according to claim 1, wherein the reusable fiber base material is a linear fiber base material or a sheet-like fiber base material.
3. The method for cultivating persimmon according to claim 1 or 2, wherein the fungus bed cultivation of persimmon is performed by bottle cultivation or bag cultivation.
4. The method for cultivating cocoons according to any one of claims 1 to 3, wherein a fiber base material is recovered from the cultivation medium after cultivating the cocoon, and the cultivating the cocoon is repeated by reusing the fiber base material as a culture base material. .
5. The method for cultivating persimmon according to any one of claims 1 to 4, wherein the persimmon is at least one selected from the group consisting of oyster mushrooms, enokitake, eringi, tamogitake, bunashimeji, shiitake and maitake.
6. The persimmon according to any one of claims 1 to 5, wherein the persimmon is at least one selected from the group consisting of enokitake mushroom, oyster mushroom, eringi, tamogitake, and bunashimeji, and fungus bed cultivation is performed in bottle cultivation. Cultivation method.
7. The method for cultivating a cocoon according to any one of claims 1 to 5, wherein the cocoon is at least one selected from the group consisting of shiitake mushroom, bamboo shoot, oyster mushroom, and maitake. .
8. 茸 A cultivation medium for strawberries, which contains a reusable fiber base, water, and nutrient sources.
9. The culture medium for cultivation of strawberries according to claim 8, wherein the reusable fiber base material is a linear fiber base material or a sheet-like fiber base material.
10. The culture medium for cultivation of persimmons according to claim 8 or 9, which is a culture medium for bottle cultivation.
11. The culture medium for cultivation of persimmons according to claim 8 or 9, which is a culture medium for bag cultivation.
12. The culture medium for cultivating persimmon according to any one of claims 8 to 11, which is used for cultivating at least one kind of persimmon selected from the group consisting of oyster mushrooms, enokitake mushrooms, eringi, tamogitake, bunashimeji, shiitake mushrooms and maitake.
13. The culture medium for cultivation of persimmons according to claim 10, which is used for cultivation of at least one kind of persimmon selected from the group consisting of enokitake, oyster mushrooms, eringi, tamogitake, and bunashimeji.
14. The culture medium for cultivating persimmons according to claim 11, which is used for cultivation of at least one kind of persimmon selected from the group consisting of shiitake mushroom, bamboo shoot, oyster mushroom, and maitake.

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