CN102150571B - Production method for biological source theanine raw material - Google Patents
Production method for biological source theanine raw material Download PDFInfo
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- CN102150571B CN102150571B CN 201110005976 CN201110005976A CN102150571B CN 102150571 B CN102150571 B CN 102150571B CN 201110005976 CN201110005976 CN 201110005976 CN 201110005976 A CN201110005976 A CN 201110005976A CN 102150571 B CN102150571 B CN 102150571B
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Abstract
The invention discloses a production method for a biological source theanine raw material. The production method comprises the following steps of: (1) screening and sprouting tea tree seeds; (2) performing aerial fog cultivation on tea seedlings; (3) cutting roots and regenerating root systems; and (4) repeating the step (3) to circularly obtain tea tree roots which are used as the raw material for producing theanine, wherein aerial fog nutrient solution in the step (2) comprises C2H7N or C2H8ClN or C3H7NO2 and C5H9NO4 or C5H10N2O3 besides nutrients necessary to the growth of the tea seedlings, and the pH of the aerial fog nutrient solution is between 5 and 5.5. By the production method, the raw material tea tree roots for producing the theanine can be obtained throughout the year, and a special formula of the tea tree growth nutrient solution is used to effectively improve the content of the theanine in the tea tree root systems; and the tea seedlings obtained by the aerial fog cultivation can be repeatedly harvested and repeatedly grow so as to realize industrial operation for the production of the tea tree roots throughout the year.
Description
Technical field
The present invention relates to a kind of production method of biological source theanine raw material.
Background technology
Theanine is tea tree characteristic nonprotein amino acid, and is soluble in water for the white needles body, has sweet taste and bright refreshing flavor, is the component of the flavour of tealeaves, also is widely used in the flavor improvement of food.Modern medicine confirms that theanine has many-sided health-care effect to human body, and especially theanine can be calmed the nerves pneumostatic and be had no side effect, and is the ideal medicament of Cure of depression.
The source of biological tea propylhomoserin comprises that extraction separates theanine, cell culture method, hair shape root cultivation, microbe fermentation method etc. from the waste liquid of tealeaves or preparation Tea Polyphenols at present.Not only cost is high to extract theanine from tealeaves, and fights for raw material with the commodity Tea Production; From Tea Polyphenols pulp thickening theanine, theanine is by product, can not realize the production of industrialization theanine; Cell culture method and hair shape root cultivation Genomic instability cause theanine output unstable; Microbial fermentation is produced theanine and is had the advantages such as be not subjected to resource limitation, there is no environmental pollution, can produce in a large number, product safety is reliable, but it requires the high degree of sterilization environment, early stage, equipment investment and later stage technical operation cost were higher, operability is not strong, particularly the producer of basic unit is difficult to grasp and operation, is difficult to promote the use in vast rural area.Proved now in tea tree that theanine is synthetic by root system, and root system especially fibrous root contain more theanine, but due to tea tree planting in soil, can't obtain tree root, get root if dig out tea tree, be also disposable, can not regenerate.The aerial fog cultivation method is a kind of planting technology of water saving the most, only need 1/10 water yield of soil cultivation, just can complete the growth of identical biomass, it is the highest production technology of water-saving agriculture water utilization rate, it is also a kind of fertile planting technology that saves most, the root system of aerial fog cultivation plant is fixed wtih in the mode that hangs in the sky, and has the most sufficient oxygen atmosphere, so high to the absorption efficiency of mineral ions fertilizer; Simultaneously, the aerial fog cultivation method reduces more than 75% insect pest, if it is isolated to carry out the tight fly net in space, can accomplish the pesticide producing of exempting from without wormization, and that can produce almost Perfect exempts from the chemistry security agricultural product.The patent of publication number CN 1308846A namely discloses a kind of use aerial fog cultivation method and has carried out the tea tree cultivation and can reach production high-quality, the purpose of high yield tealeaves, but tea root production and that contain higher theanine content that can't obtain circulating, thereby the tea root of producing of therefore can not realizing circulating is the purpose that raw material carries out large-scale production biological tea propylhomoserin.
Summary of the invention
The root that the object of the invention is to use the aerosol that contains special dietary to spray the tea seed that germinates makes it grow at short notice the flourishing root system that contains more theanine, root is cut as the raw material of producing theanine, continuing air culture after pruning stimulates tea shoot to grow new root system, cut longly, circulation is produced again again.
The production method of biological source theanine raw material of the present invention, it comprises the steps:
(1) screening of tea tree seed and vernalization: comprise seed soaking, seed selection, sterilization, four steps of vernalization;
(2) cultivation of tea shoot: when the seed radicle of receiving guest with tea grows to few 1cm, transplant tea seed embryo make bud, leaf outwardly root be fixed on mist training bed inwardly and carry out that root system is exposed, unsettled, the aerial fog cultivation of full shading, 10 ℃ ~ 38 ℃ of culture environment temperature, humidity 60% ~ 90%; Aerosol nutrient solution EC value 0.4 ~ 1.6,15 ℃ of temperature; 15 ℃ ~ 28 ℃ of rhizosphere temperature, humidity 80% ~ 95%; Except containing the necessary nutrient component of tea shoot growth, also contain C in described aerosol nutrient solution
2H
7N(ethamine) or C
2H
8The ClN(ethylamine hydrochloride) or C
3H
7NO
2(alanine) and C
5H
9NO
4(glutamic acid) or C
5H
10N
2O
3(glutamine), and aerosol nutrient solution pH5 ~ 5.5;
(3) cut root and root regeneration: when tea root is longer, the distance foundation at least 3cm cut tea root in the place; Tea shoot after cutting root continues to adopt the described aerosol cultural method of step (2) to cultivate;
(4) repeating step (3), circulation obtains tea root, as the raw material of theanine production.
Nutrient composition C described in step of the present invention (2)
2H
7N or C
2H
8ClN or C
3H
7NO
21 ~ 10g/L, C
5H
9NO
4Or C
5H
10N
2O
3100 ~ 500 mg/L; The necessary nutrient component of the tea shoot that contains in described nutrient solution growth comprises that n p k nutrition composition and the mineral salt plant that becomes to grade cultivates the component of being commonly used, and for example can be (NH
4)
2SO
430 ~ 140mg/L, NH
4NO
37 ~ 30mg/L, KH
2PO
45 ~ 14mg/L, K
2SO
420 ~ 90mg/L, CaCl
210 ~ 85mg/L, MgSO
47H
2O 65 ~ 250mg/L, Al
2(SO
4)
318H
2O 60 ~ 250mg/L, ZnSO
47H
2O 110 ~ 440mg/L, H
3BO
3140 ~ 560mg/L, H
2SiO
3100 ~ 800 mg/L, CuSO
45H
2O 25 ~ 100mg/L, MnSO
4H
2O 18 ~ 70mg/L, Na
2MoO
42H
2O 15 ~ 60mg/L, Fe-EDTA 85 ~ 350mg/L.
Described in step in the present invention (2), the aerosol incubation can be controlled by the computer controlled automatic device, be provided with sensing device in described automaton and detect data in order to receiving sensor, and carry out data and process, control the central processing unit of execution unit action; Sensing device is by being arranged on the rhizosphere Temperature Humidity Sensor of Rhizosphere Soils in Tea Garden for detection of Rhizosphere Soils in Tea Garden surface moisture film and moisture content distribution, consisting of for detection of the aerial temperature and humidity sensor of ambient temperature and humidity with for detection of nutrient solution EC value, the temperature sensor of tea tree nutrient solution electrical conductivity and temperature; The signal output part of rhizosphere Temperature Humidity Sensor, aerial temperature and humidity sensor and nutrient solution EC value, temperature sensor is connected with the central processing unit signal input interface by shielded line with the signal input part of central processing unit, and the signal output part of central processing unit is connected with the signal input part of execution unit.
In step of the present invention (3) preferably when the long 20-80cm of tea root, the distance foundation at least 3cm cut tea root in the place.
Beneficial effect of the present invention:
1, with the raw material of tea root as production biogenetic derivation theanine, open up theanine production new way.Can obtain theanine raw materials for production tea root through cultivation method of the present invention, and theanine content increases significantly throughout the year.
2, adopt special growth of tea plant nutrient solution prescription, effectively improve theanine content in tea root.
3, the aerial fog cultivation tea shoot can repeatedly gather in, repeatedly grow, thereby realizes producing throughout the year the factory operation of tea root throughout the year.
4, the nutrient solution of aerosol cultivation tea tree is completed the supply of nutrient and moisture in a tight locking type circulatory system, thereby realizes that zero effluxes, and accomplishes sustainable free from environmental pollution.
5, aerial fog cultivation is produced the theanine raw material, has realized producing without waste material, refuse and waste liquid, and the root system that grows is clean, is convenient to storage, transportation and processing, is a kind of continuable permanent industrialized production in agriculture mode of production.
6, can be carried out by computer control the aerial fog cultivation of tea tree, realized the diversification accuracy controlling of growth of tea plant, for best environmental condition has been created in the tea root growth, temperature and humidity, nutrient solution concentration and the temperature, tea shoot environmental temperature and the humidity that comprise rhizosphere environment, make the growth of tea root considerably beyond soil cultivation, greatly improve root growth efficient.
7, rely on computer controlled automatic to carry out aerosol cultivation of tea tree, simple to operate, easily study is easily grasped, and is convenient to demonstrate in vast rural area and promote.
Description of drawings
Accompanying drawing is the computer controlled automatic apparatus structure schematic block diagram that uses in the present invention.
Embodiment
Embodiment 1
Tea tree breed is Shu Chazao.Collect seed mid-March in the tea place, begin experiment mid-April.Seed soaking: wash seed by rubbing with the hands 3 times with running water and soak 7d with slow flowing water afterwards; Seed selection and sterilization: select seed full, that sink to the bottom to pull out, use 0.1%HgCl
2Soak the 20min sterilization, then use distilled water flushing 3 times; Vernalization: seed is sown into is equipped with in the thick perlitic vernalization alms bowl of 10cm, cover with moist perlite, then cover with vernalization alms bowl lid with holes, be placed in the vernalization of alternating temperature seed germination box, 20 ℃ ~ 30 ℃ of day temperatures, night, temperature was 15 ℃ ~ 22 ℃.Suitably water spray makes perlite humidity 40% ~ 60% later on.
During the long 1 ~ 2cm of the seed radicle of receiving guest with tea, with width approximately the sponge strip of 1cm bind seedling, bud, leaf root outwardly are fixed on mist training bed surface inwardly and carry out that root system is exposed, unsettled, the aerosol of full shading is cultivated.The cultivation process is undertaken by computer controlled automatic, is provided with sensing device in described computer controlled automatic device and detects data in order to receiving sensor, and carry out data and process, and controls the central processing unit of execution unit action; Sensing device is by being arranged on the rhizosphere Temperature Humidity Sensor of Rhizosphere Soils in Tea Garden for detection of Rhizosphere Soils in Tea Garden surface moisture film and moisture content distribution, consisting of for detection of the aerial temperature and humidity sensor of ambient temperature and humidity with for detection of nutrient solution EC value, the temperature sensor of tea tree nutrient solution electrical conductivity and temperature; The signal input part of rhizosphere Temperature Humidity Sensor, aerial temperature and humidity sensor and nutrient solution EC value, temperature sensor is connected with the central processing unit signal input interface by shielded line, and the signal output part of central processing unit is connected with the signal input part of execution unit; In conjunction with each temperature sensor, the calibrating high temperature limit is when excess Temperature, spraying reduces temperature continuously, for Root growing space is created suitable temperature, the electrical conductivity EC value sensor of nutrient solution, measure EC value in phegma, add concentrated mother liquor as the nutrient solution concentration monitoring parameter in good time; 10 ℃ ~ 38 ℃ of the temperature that controls environment, humidity 60% ~ 90%; Nutrient solution EC value 0.4 ~ 1.6,15 ℃ of temperature; 15 ℃ ~ 28 ℃ of rhizosphere temperature, humidity 80% ~ 95%; Nutrient composition C
2H
8ClN 5g/L, C
5H
9NO
4500 mg/L, H
2SiO
3500 mg/L, (NH
4)
2SO
4140mg/L, NH
4NO
330mg/L, KH
2PO
414mg/L, K
2SO
490mg/L, CaCl
285mg/L, MgSO
4.7H
2O 250mg/L, Al
2(SO
4)
3.18H
2O 200mg/L, ZnSO
4.7H
2O 200 mg/L, H
3BO
3500mg/L, CuSO
4.5H
2O 90mg/L, MnSO
4.H
2O 70mg/L, Na
2MoO
4.2H
2O 60mg/L, Fe-EDTA 350mg/L.pH5.5。
At the beginning of 6 months, the tea shoot root is about 50cm, cuts tea root at distance foundation 5cm place, the raw material of producing as theanine.And tea shoot continues to cultivate after cutting root, can grow new root.So circulation shear, can obtain tea root repeatedly, and cultivate through aerosol 4% left and right that contained theanine in the gained root system accounts for the dry total amount.
Embodiment 2
Tea tree breed is Qimen paper mulberry leaf kind.Collect seed mid-March in the tea place, begin experiment mid-April.Seed soaking: wash seed by rubbing with the hands 3 times with running water and soak 7d with slow flowing water afterwards; Seed selection and sterilization: select seed full, that sink to the bottom to pull out, use 0.1%HgCl
2Soak the 20min sterilization, then use distilled water flushing 3 times; Vernalization: seed is sown into and is equipped with in the thick perlitic vernalization alms bowl of 10cm, covers with moist perlite, then covers with vernalization alms bowl lid with holes, is placed in the vernalization of alternating temperature seed germination box, 20 ℃ ~ 30 ℃ of day temperatures, and night, temperature was 15 ℃ ~ 22 ℃.Suitably water spray makes perlite humidity 40% ~ 60% later on.
During the long 1 ~ 2cm of the seed radicle of receiving guest with tea, with width approximately the sponge strip of 1cm bind seedling, bud, leaf root outwardly are fixed on mist training bed inwardly and carry out aerosol and cultivate; The cultivation process is undertaken by computer controlled automatic, is provided with sensing device in described computer controlled automatic device and detects data in order to receiving sensor, and carry out data and process, and controls the central processing unit of execution unit action; Sensing device is by being arranged on the rhizosphere Temperature Humidity Sensor of Rhizosphere Soils in Tea Garden for detection of Rhizosphere Soils in Tea Garden surface moisture film and moisture content distribution, consisting of for detection of the aerial temperature and humidity sensor of ambient temperature and humidity with for detection of nutrient solution EC value, the temperature sensor of tea tree nutrient solution electrical conductivity and temperature; The signal output part of rhizosphere Temperature Humidity Sensor, aerial temperature and humidity sensor and nutrient solution EC value, temperature sensor is connected with the central processing unit signal input interface by shielded line with the signal input part of central processing unit, and the signal output part of central processing unit is connected with the signal input part of execution unit; In conjunction with each temperature sensor, the calibrating high temperature limit is when excess Temperature, spraying reduces temperature continuously, for Root growing space is created suitable temperature, the electrical conductivity EC value sensor of nutrient solution, measure EC value in phegma, add concentrated mother liquor as the nutrient solution concentration monitoring parameter in good time; 10 ℃ ~ 38 ℃ of the temperature that controls environment, humidity 60% ~ 90%; Nutrient solution EC value 0.4 ~ 1.6,15 ℃ of temperature; 15 ℃ ~ 28 ℃ of rhizosphere temperature, humidity 80% ~ 95%; Nutrient composition C
2H
7N 5g/L, C
5H
10N
2O
3300 mg/L, H
2SiO
3100 mg/L, (NH
4)
2SO
450mg/L, NH
4NO
310mg/L, KH
2PO
45mg/L, K
2SO
420mg/L, CaCl
215mg/L, MgSO
4.7H
2O 80mg/L, Al
2(SO
4)
3.18H
2O 60mg/L, ZnSO
4.7H
2O 400 mg/L, H
3BO
3150 mg/L, CuSO
4.5H
2O 25 mg/L, MnSO
4.H
2O 20mg/L, Na
2MoO
4.2H
2O 20 mg/L, Fe-EDTA 90mg/L.pH5.5。
At the beginning of 6 months, the tea shoot root is about 50cm, cuts tea root at distance foundation 4cm place, the raw material of producing as theanine.And tea shoot continues to cultivate after cutting root, can grow new root.So circulation shear, can obtain tea root repeatedly, and cultivate through aerosol 3% left and right that contained theanine in the gained root system accounts for the dry total amount.
Embodiment 3
Tea tree breed is that the hillside is green.Collect seed mid-March in the tea place, begin experiment mid-April.Seed soaking: wash seed by rubbing with the hands 3 times with running water and soak 7d with slow flowing water afterwards; Seed selection and sterilization: select seed full, that sink to the bottom to pull out, use 0.1%HgCl
2Soak the 20min sterilization, then use distilled water flushing 3 times; Vernalization: seed is sown into is equipped with in the thick perlitic vernalization alms bowl of 10cm, cover with moist perlite, then cover with vernalization alms bowl lid with holes, be placed in the vernalization of alternating temperature seed germination box, 20 ℃ ~ 30 ℃ of day temperatures, night, temperature was 15 ℃ ~ 22 ℃.Suitably water spray makes perlite humidity 40% ~ 60% later on.
During the long 1 ~ 2cm of the seed radicle of receiving guest with tea, with width approximately the sponge strip of 1cm bind seedling, bud, leaf root outwardly are fixed on a tight locking type mist training bed surface inwardly and carry out aerial fog cultivation.The cultivation process is undertaken by computer controlled automatic, is provided with sensing device in described computer controlled automatic device and detects data and carry out the central processing unit that data are processed, controlled the execution unit action in order to receiving sensor; Sensing device is by being arranged on the rhizosphere Temperature Humidity Sensor of Rhizosphere Soils in Tea Garden for detection of Rhizosphere Soils in Tea Garden surface moisture film and moisture content distribution, consisting of for detection of the aerial temperature and humidity sensor of ambient temperature and humidity with for detection of nutrient solution EC value, the temperature sensor of tea tree nutrient solution electrical conductivity and temperature; The signal output part of rhizosphere Temperature Humidity Sensor, aerial temperature and humidity sensor and nutrient solution EC value, temperature sensor is connected with the central processing unit signal input interface by shielded line with the signal input part of central processing unit, and the signal output part of central processing unit is connected with the signal input part of execution unit; In conjunction with each temperature sensor, the calibrating high temperature limit is when excess Temperature, spraying reduces temperature continuously, for Root growing space is created suitable temperature, the electrical conductivity EC value sensor of nutrient solution, measure EC value in phegma, add concentrated mother liquor as the nutrient solution concentration monitoring parameter in good time; Keep 10 ℃ ~ 38 ℃ of environmental temperatures, humidity 60% ~ 90%; Nutrient solution EC value 0.4 ~ 1.6,15 ℃ of temperature; 15 ℃ ~ 28 ℃ of rhizosphere temperature, humidity 80% ~ 95%; Nutrient composition C
3H
7NO
210g/L, C
5H
9NO
4500 mg/L, H
2SiO
3500 mg/L, (NH
4)
2SO
4140mg/L, NH
4NO
330mg/L, KH
2PO
414mg/L, K
2SO
490mg/L, CaCl
285mg/L, MgSO
4.7H
2O 250mg/L, Al
2(SO
4)
3.18H
2O 200mg/L, ZnSO
4.7H
2O 200 mg/L, H
3BO
3500 mg/L, CuSO
4.5H
2O 90 mg/L, MnSO
4.H
2O 70mg/L, Na
2MoO
4.2H
2O 60 mg/L, Fe-EDTA 350mg/L.pH5.5。
At the beginning of 6 months, the tea shoot root is about 50cm, cuts tea root at distance foundation 5cm place, the raw material of producing as theanine.And tea shoot continues to cultivate after cutting root, can grow new root.So circulation shear, can obtain tea root repeatedly, and cultivate through aerosol 2% left and right that contained theanine in the gained root system accounts for the dry total amount.
Claims (2)
1. the production method of a biological source theanine raw material, is characterized in that: comprise the steps:
(1) screening of tea tree seed and vernalization: comprise seed soaking, seed selection, sterilization, four steps of vernalization;
(2) cultivation of tea shoot: when the seed radicle of receiving guest with tea grows to few 1cm, transplant tea seed embryo make bud, leaf outwardly root be fixed on mist training bed inwardly and carry out that root system is exposed, unsettled, the aerial fog cultivation of full shading, 10 ℃ ~ 38 ℃ of culture environment temperature, humidity 60% ~ 90%; Aerosol nutrient solution EC value 0.4 ~ 1.6,15 ℃ of temperature; 15 ℃ ~ 28 ℃ of rhizosphere temperature, humidity 80% ~ 95%; Except containing the necessary nutrient component of tea shoot growth, also contain C in described aerosol nutrient solution
2H
7N or C
2H
8ClN or C
3H
7NO
21 ~ 10g/L, C
5H
9NO
4Or C
5H
10N
2O
3100 ~ 500 mg/L, and aerosol nutrient solution pH5 ~ 5.5;
(3) cut root and root regeneration: when the long 20-80cm of tea root, the distance foundation at least 3cm cut tea root in the place; Tea shoot after cutting root continues to adopt the described aerial fog cultivation method of step (2) to cultivate;
(4) repeating step (3), circulation obtains tea root, as the raw material of theanine production.
2. the production method of biological source theanine raw material as claimed in claim 1, it is characterized in that: described in step (2), the aerosol incubation is controlled by the computer controlled automatic device, be provided with sensing device in described automaton and detect data in order to receiving sensor, and carry out data and process, control the central processing unit of execution unit action; Sensing device is by being arranged on the rhizosphere Temperature Humidity Sensor of Rhizosphere Soils in Tea Garden for detection of Rhizosphere Soils in Tea Garden surface moisture film and moisture content distribution, consisting of for detection of the aerial temperature and humidity sensor of ambient temperature and humidity with for detection of nutrient solution EC value, the temperature sensor of tea tree nutrient solution electrical conductivity and temperature; The signal output part of rhizosphere Temperature Humidity Sensor, aerial temperature and humidity sensor and nutrient solution EC value, temperature sensor is connected with the central processing unit signal input interface by shielded line with the signal input part of central processing unit, and the signal output part of central processing unit is connected with the signal input part of execution unit.
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| CN108634068A (en) * | 2018-07-09 | 2018-10-12 | 黄山学院 | Tea root tea and its processing technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148113C (en) * | 2000-11-22 | 2004-05-05 | 成都茜亚现代农业发展有限责任公司 | Bare root and atomization technique for cultivating tea tree |
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2011
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148113C (en) * | 2000-11-22 | 2004-05-05 | 成都茜亚现代农业发展有限责任公司 | Bare root and atomization technique for cultivating tea tree |
Non-Patent Citations (1)
| Title |
|---|
| 徐伟忠等.蔬菜工厂－芽苗菜智能化栽培技术.《蔬菜工厂－芽苗菜智能化栽培技术》.台海出版社,2006,(第1版),10-37. * |
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