CN105660709B - A kind of method for the accelerating agent of Chinese yew root growth and its for crossbreeding - Google Patents
A kind of method for the accelerating agent of Chinese yew root growth and its for crossbreeding Download PDFInfo
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- CN105660709B CN105660709B CN201610094131.XA CN201610094131A CN105660709B CN 105660709 B CN105660709 B CN 105660709B CN 201610094131 A CN201610094131 A CN 201610094131A CN 105660709 B CN105660709 B CN 105660709B
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- accelerating agent
- bacillus
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- chinese yew
- zymotic fluid
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- 241001149649 Taxus wallichiana var. chinensis Species 0.000 title claims abstract description 29
- 230000002786 root growth Effects 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title abstract description 15
- 238000009402 cross-breeding Methods 0.000 title abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 24
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 24
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- 229930191978 Gibberellin Natural products 0.000 claims abstract description 10
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- 239000003448 gibberellin Substances 0.000 claims abstract description 10
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims abstract description 5
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- 229920000742 Cotton Polymers 0.000 description 3
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of accelerating agent for Chinese yew root growth and its method for crossbreeding, the accelerating agent includes Candida fermented liquid, the lichen bacillus ferments liquid, Cellumomonas flavigena zymotic fluid, Bacillus foecalis alkaligenes zymotic fluid, gibberellin, activated carbon, dipotassium hydrogen phosphate.Accelerating agent of the present invention can promote Chinese yew tissue-cultured seedling Rapid Rooting, increase survival rate, which has a extensive future for the deep development of Resource of Taxus L and extensively using having important and far-reaching meaning.
Description
Technical field
The present invention relates to agriculture and forestry technical field, and in particular to a kind of accelerating agent and its use for Chinese yew root growth
In the method for crossbreeding.
Background technology
Chinese yew, also known as Japanese yew, belong to the rare tree of first-grade state protection, and wildwood resource is exhausted.Chinese yew is normal
Green arbor, trunk lead to directly, and tree performance is graceful, and when seed maturity takes on a red color, and imitation leather is dazzlingly beautiful, is excellent potted landscape and greening-tree
Kind.Chinese yew timber is sturdy and durable, and texture is beautiful, and root, stem are all high-grade material for carving on it, is to belong to extraordinary to use woods material；It
Root, stem, skin, leaf contain taxol, are the curing cancer drugs that the world today generally acknowledges, leaf can promoting menstruation, diuresis, blood pressure lowering, to prevention
Diabetes and angiocardiopathy have positive effect, are the expensive Medical forests of value.In recent years Chinese year potted landscpe is also exhaled in flowers and trees market
Be intended to out, become the upstart to attract people's attention.Chinese year potted landscpe is placed on the indoor effect for having obvious expelling mosquito and purifying air
The harmful substance such as fruit, its absorbing carbon dioxide, carbon monoxide, nicotine, sulfur dioxide, moreover it is possible to absorb formaldehyde, benzene, toluene, two
The carcinogens such as toluene, breathe out oxygen, play cancer-resisting health care and air purification effect, be family room, hotel, hotel and
The rare species that office space is beautified the environment.
Prior art literature 1CN1194603C discloses a kind of artificial cultivation method of the Chinese yew, " measure taken
It is：A, in clay, composite fertilizer is added；B, organic fertilizer is added again, forms loam；C, keep soil water content 35~
45%；D, transplant：Selection Chinese yew rest period to Chinese yew lateral bud starts before expanding, and is transplanted in rainy days.It is described multiple
The composition in Hefei is：Finland import chlorination ternary 16-16-16, total nutrient >=45%；The composition of the organic fertilizer raw material is mainly
Birds droppings and/or pig manure and/or chicken manure；During preparation：A, first ferment：Birds droppings and/or pig manure and/or chicken manure are covered on the container of sealing
In Nylon Bag, allow it to ferment automatically, generally require 2 months or so time；B, dry again：Organic fertilizer after fermentation is passed through into light
Cooperation is used, and is dried to just can be into untill powdered with hand rubbing；C, it is finely ground again：It is using roller that the organic fertilizer after drying is fine ground to powder
Last shape.The light application time of Chinese yew seedling in summer intense light irradiation, it is daily 3~4 it is small when：The light application time with spring is in autumn
5~6 it is small when.It must keep the root system integrality of treelet during transplanting and the moisture content of Gen Mao reaches 50---60%, during transplanting
Ensure that root system and soil are combined closely, and water and determine root.During transplanting, the height of seedling first will more than 20 centimetres before transplanting
Carry out the training and moulding of Chinese yew seedling.Before transplanting, for Chinese yew growth of seedling in greenhouse, greenhouse can be with sunshade, moisturizing, light
According to appropriateness.Training depend on the circumstances, it is necessary to transplant potted landscape pruned by bonsai style, it is necessary to large area plantation moulding
The plate field of unified specification may be selected, form one of beautiful scenery, it is also possible to other moulding.After planting yew is good, often
April in year and August are earthed up twice, and composite fertilizer and organic fertilizer should be added in clay in ridging.Organic fertilizer is added to loam
When, fertilising quantity is 10 grams of 100 grams of every plant of organic fertilizer and composite fertilizer；Or fertilising quantity is 5 grams of 100 scholar of more plants of organic fertilizer and compound
Fertile 2 grams of 101 scholar.”
The above method is unsatisfactory in efficiency and final survival rate, and the growth and development of root system of plant is to improving plant
Nutrient absorption ability, the increase of biomass and plant quality play the role of important, and as the accelerating agent of root system of plant, take root
Powder is widely used in the production of agriculture and forestry, generates huge economic benefit in production.Root-inducing powder in existing patent
Preparation promotes the extension of root system of plant by using a variety of such as methyl α-naphthyl acetates, indolebutyric acid plant growth regulator, to planting
Thing has no the support in nutrition, while excessive use may cause the normal growth and development of plant to be disturbed, and influence to blossom and bear fruit.
For this reason, the application forms microecosystem by complex microorganism, reasonable compatibility between each strain, symbiosis is coordinated,
Mutually antagonism, microorganism secretion root system regulator and ablastins can not take good care of the rotten mould root rot of seedlings root infection, phytophthora root-rot
Sick, bacillary root rot and the sprout term disease such as base rot disease and damping-off, samping off, avoid simple plant growth regulator
Use, and supplement is imposed to the nutritional deficiency of plant, while the rooting rate for solving existing root-inducing powder is low, quantity of taking root is few and takes root
The technical problem of time length, there is provided a kind of accelerating agent of Chinese yew root growth and its method for crossbreeding, to red
The cultivation transplanting of beans China fir has great importance.
The content of the invention
The technical problem to be solved by the present invention is to provide a kind of accelerating agent for Chinese yew root growth and its for miscellaneous
Hand over breeding method, solves the problems, such as the Taxus plant inter-species florescence it is asynchronous cause pollination breeding it is difficult.
In order to achieve the above object, the present invention provides a kind of accelerating agent for Chinese yew root growth and its for miscellaneous
The method for handing over breeding, includes the following steps：
（1）Pollen collection：In 2 months, southerm yew is gathered respectively（Taxus wallichiana var. mairei）
And Taxus x media（Taxus × media）Staminiferous plant pollen, spontaneously dry, reject impurity after, be respectively placed in EP pipes 4
DEG C refrigeration is spare, and keeps drying；
（2）Pollination：To March, female plant enters the guttation phase, takes out the pollen of storage, room temperature, which is placed one hour, makes its recovery
Room temperature；According to pollen：Ultra-pure water=1:1000 part by weight is configured to pollen liquid, pollinates according to southerm yew pollen graceful
Ground Asia female plant, Taxus x media pollen pollination southerm yew female plant, is sprayed female plant, was sprayed every four days using watering can respectively
Once, pollinate 3 times altogether；Single plant processing can be stained with pollen smearing guttation phase female flower using sterilized cotton swabs and be pollinated；Own after pollination
Pollination plant carries out plant division isolation to prevent the interference of foreign pollen.
（3）Fertilising：After pollination, fertilising, is 1 according to nitrogen, phosphorus, potassium ratio:3:1, spray 3 foliar fertilizers；
（4）Bear fruit：Spray nutritious liquor after bearing fruit, nutrient composition are：N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer
With NH4H2PO4Form adds, and potash fertilizer is with KNO3Form adds, nitrogenous fertilizer divided by NH4H2PO4And KNO3Form add outer, not foot
Divide and use（NH4）2SO4 Supplement.
Ensure plant nutrition supply；After seed maturity, different male parent pollination breeding kinds are gathered from maternal plant respectively
Son, obtains purebred and cenospecies seed；
（5）Sand storage：By the seed soaked in cold water 5 days of collection, water is changed daily once, the surface of the seed moisture dries in the air after seed soaking
It is dry, low temperature sand storage, by sand：Seed 3:1 carries out；
（6）Sowing：Spring, after the Seed sterilization of sand storage, with 280mg/L GA3+20mg/L 6-BA +25mg/L KT+
When 8mg/L ABT immersion seeds 24 are small, rear sowing, obtains in vitro cuttings；
（7）Transplanting：By in vitro cuttings hardening 10~15 days, it is then taken out, the culture medium of root is cleaned, by its root
Portion is immersed in 30min in accelerating agent, promotes the growth of Chinese yew plant new root.
The accelerating agent includes the raw material of following weight proportion：Candida fermented liquid：The lichen bacillus ferments liquid：
Cellumomonas flavigena zymotic fluid：Bacillus foecalis alkaligenes zymotic fluid：Gibberellin：Activated carbon：Dipotassium hydrogen phosphate=5：3：2：3：1：2：
1；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276；
The Cellumomonas flavigena is Cellumomonas flavigena（cellulomonas flavigena）ATCC
No.482；
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes（Alcaligenes faecalis）ATCC 31555；
The preparation method of the accelerating agent is：First by candidiasis, bacillus licheniformis, Cellumomonas flavigena,
Bacillus foecalis alkaligenes activates in a conventional manner respectively, then cultivates into bacterium solution viable count and reaches 108A/gram acquisition zymotic fluid,
By above-mentioned zymotic fluid according to mass ratio 5：3：2：3 mixing, according to weight proportion addition activated carbon, gibberellin and dipotassium hydrogen phosphate
To obtain the final product.
Survival rate is to 97.1% after transplanting 30 days.
Cross breeding method of the present invention has the following advantages：
1st, hybrid strain：
Plant hybridization breeding field, most important difference are that the different selections for each parent being directed to, and selection is different
Parent must just carry out multiplicating experiment to the breeding method of specific cross combination, different original interspecific hybridization abortions are very
Height, after hybridization, although there is higher fruit rate, also has seed, does not have embryo inside seed after detection inside fruit.Southern red bean
The China fir speed of growth is fast, content of taxol is low；The Taxus x media speed of growth is slow, and content of taxol is high.Chinese yew is that different flower is awarded
Powder plant.Southerm yew hybridizes with Taxus x media, obtains filial generation, it is had parents' advantage concurrently, can effectively solve
The problem of certainly taxol pharmaceutical raw material is short, and obtain the F1 generation seed germination rate of cross combination, fertile rate highest；
2nd, first pre-processed before lamination with cold water, since stratification is a slow water absorption course, be also at the same time
One process for slowly converting and slowly germinateing, Seed sprouting is related to seed moisture proportion, and only seed-bearing moisture content approaches
Or just start to sprout when reaching saturated aqueous rate, and reach containing when starting lamination with seed saturated aqueous rate required time
Water rate is related, and moisture content is lower before lamination, and water suction is slower, reaches that the time of saturated aqueous rate is longer, the time of suitable vernalization
Also can be longer；Inventor is found through experiments that, seed is carried out soaked in cold water 5 days before lamination, changes water daily once, such pre-
After processing, lamination can be caused just to reach within 60 days or so saturation state, shorten germination time, and the pretreatment can increase field
Germination percentage.
3rd, the growth and development of hormone kind and concentration to seed plays the role of highly significant, due to Chinese yew genus plants seed
Difficulty is sprouted, the application is had found by many experiments, with 280mg/L GA3+20mg/L 6-BA +25mg/L KT+8mg/L
When ABT immersion seeds 24 are small, rear sowing, can significantly improve the germination percentage of hybrid seed, single factor experiment the result shows that, use
After hormone immersion, percentage of seedgermination improves 36% or so, and substantially reduces sprout time.
When the 4th, transplanting, the moderate concentration of gibberellin, stimulates the stretching, extension of root cell vigorous, collocation candidiasis, lichens
Bacillus, Cellumomonas flavigena, Bacillus foecalis alkaligenes form a good microecosystem, rationally match somebody with somebody between each strain
5, symbiosis is coordinated, and mutually antagonism, microorganism secretion root system regulator and ablastins can not take good care of the rotten mould root-rot of seedlings root infection
Disease, phytophthora root rot, bacillary root rot and the sprout term disease such as base rot disease and damping-off, samping off, so as to effectively control seedling
Rotten phenomenon of dead seedling of phase, dipotassium hydrogen phosphate continually supply nutrition to root system, maintain the strong robust seedling impetus of seedling, greatly improve
The adaptability of plant, improves whole survival rate.
Embodiment
Embodiment 1
A kind of accelerating agent is used for the cross breeding method of Chinese yew, includes the following steps：
（1）Pollen collection：Determine southerm yew（Taxus wallichiana var. mairei）With the sub- red bean in graceful ground
China fir（Taxus × media）Male and female plant, and investigate the specific time at florescence.It turns out that the first flower of southerm yew male flower
Phase, the early flowering season of female flower was basically identical than early more than 10 days of Taxus x media；The guttation phase 2 of two kinds of Chinese yew female flower single flowers
My god, whole strain female flower guttation phase left and right for 2 weeks.The pollen of the staminiferous plant of late Febuary collection Taxus x media, spontaneously dries, picks
Except impurity, to be placed on the 4 DEG C of refrigerations of EP pipes spare, and keeps drying.
（2）Pollination：From March 1, female flower guttation situation on southerm yew and Taxus x media female plant is observed, it is spontaneous
Existing female flower starts to take out within second day the pollen stored into the guttation phase, and room temperature, which is placed, makes its recover room temperature for one hour；According to flower
Powder：Ultra-pure water=1：1000 proportional arrangement is into pollen liquid, by Taxus x media pollen pollination southerm yew female plant, spray
Female plant is applied, was sprayed once using watering can every four days, is pollinated 3 times altogether；Single plant processing can be stained with pollen smearing using sterilized cotton swabs and be spat
Water phase female flower is pollinated；All pollination plant carry out plant division isolation to prevent the interference of foreign pollen after pollination.
（3）Fertilising：After pollination, fertilising, is 1 according to nitrogen, phosphorus, potassium ratio:3:1, spray 3 foliar fertilizers；
（4）Bear fruit：Spray nutritious liquor after bearing fruit, nutrient composition are：N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer
With NH4H2PO4Form adds, and potash fertilizer is with KNO3Form adds, nitrogenous fertilizer divided by NH4H2PO4And KNO3Form add outer, not foot
Divide and use（NH4）2SO4 Supplement.
Ensure plant nutrition supply；After seed maturity, hybrid seed is gathered；
5）Sand storage：By the seed soaked in cold water 5 days of collection, water is changed daily once, dries the surface of the seed moisture after seed soaking,
Low temperature sand storage, by sand：Seed is 3:1 weight ratio carries out；
（6）Sowing：Spring, after the Seed sterilization of sand storage, with 280mg/L GA3+20mg/L 6-BA +25mg/L KT+
When 8mg/L ABT immersion seeds 24 are small, rear sowing, obtains in vitro cuttings；
（7）Transplanting：By in vitro cuttings hardening 10~15 days, it is then taken out, the culture medium of root is cleaned, by its root
Portion is immersed in 30min in accelerating agent, promotes the growth of southerm yew plant new root；
The accelerating agent includes the raw material of following weight proportion：Candida fermented liquid：The lichen bacillus ferments liquid：
Cellumomonas flavigena zymotic fluid：Bacillus foecalis alkaligenes zymotic fluid：Gibberellin：Activated carbon：Dipotassium hydrogen phosphate=5：3：2：3：1：2：
1；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276；
(referring to CN101215537A);
The Cellumomonas flavigena is Cellumomonas flavigena（cellulomonas flavigena）ATCC
No.482；
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes（Alcaligenes faecalis）ATCC 31555；
The preparation method of the accelerating agent is：First by candidiasis, bacillus licheniformis, Cellumomonas flavigena,
Bacillus foecalis alkaligenes activates in a conventional manner respectively, then cultivates into bacterium solution viable count and reaches 108A/gram acquisition zymotic fluid,
By above-mentioned zymotic fluid according to mass ratio 5：3：2：3 mixing, according to weight proportion addition activated carbon, gibberellin and dipotassium hydrogen phosphate
To obtain the final product.
Survival rate is to 97.1% after transplanting 30 days, and all survive plant equal winter and resist cold；
The southerm yew plant obtained through the method for the present invention, growing way is strong, disease-resistant, can pass the winter safely, effectively solves
The vacancy of Chinese yew market resource, has broad application prospects.
The Chinese yew cultivated according to the method for the present invention, growth cycle is fast, and branches and leaves can be harvested at 2 years or so and are used to carry
Take taxol.
Embodiment 2
Accelerating agent is used for the genetic and breeding method of Taxus x media, includes the following steps：
（1）Pollen collection：Determine southerm yew（Taxus wallichiana var. mairei）With the sub- red bean in graceful ground
China fir（Taxus × media）Male and female plant, and investigate the specific time at florescence.It turns out that the first flower of southerm yew male flower
Phase, the early flowering season of female flower was basically identical than early more than 10 days of Taxus x media；The guttation phase 2 of two kinds of Chinese yew female flower single flowers
My god, whole strain female flower guttation phase left and right for 2 weeks.Gather the pollen of southerm yew staminiferous plant mid-February, late Febuary gathers gracefully
The pollen of the staminiferous plant of sub- Chinese yew, spontaneously dries, and rejecting impurity, to be placed on the 4 DEG C of refrigerations of EP pipes spare, and keeps drying.
（2）Pollination：From March 1, female flower guttation situation on southerm yew and Taxus x media female plant is observed, it is spontaneous
Existing female flower starts to take out within second day the pollen stored into the guttation phase, and room temperature, which is placed, makes its recover room temperature for one hour；According to flower
Powder：Ultra-pure water=1：1000 proportional arrangement by the sub- female plant in the graceful ground of southerm yew pollen pollination, sprays female plant into pollen liquid,
Sprayed once using watering can every four days, pollinated 3 times altogether；Single plant processing can be stained with pollen using sterilized cotton swabs and smear guttation phase female flower
Pollinate；All pollination plant carry out plant division isolation to prevent the interference of foreign pollen after pollination.
（3）Fertilising：After pollination, fertilising, is 1 according to nitrogen, phosphorus, potassium ratio:3:1, spray 3 foliar fertilizers；
（4）Bear fruit：Spray nutritious liquor after bearing fruit, nutrient composition are：N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer
With NH4H2PO4Form adds, and potash fertilizer is with KNO3Form adds, nitrogenous fertilizer divided by NH4H2PO4And KNO3Form add outer, not foot
Divide and use（NH4）2SO4 Supplement.
Ensure plant nutrition supply；After seed maturity, hybrid seed is gathered；
（5）Sand storage：By the seed soaked in cold water 5 days of collection, water is changed daily once, the surface of the seed moisture dries in the air after seed soaking
It is dry, low temperature sand storage, by sand：Seed is 3:1 weight ratio carries out；
（6）Sowing：Spring, after the Seed sterilization of sand storage, with 280mg/L GA3+20mg/L 6-BA +25mg/L KT+
When 8mg/L ABT immersion seeds 24 are small, rear sowing, obtains in vitro cuttings；
（7）Transplanting：By in vitro cuttings hardening 10~15 days, it is then taken out, the culture medium of root is cleaned, by its root
Portion is immersed in 30min in accelerating agent, promotes the growth of southerm yew plant new root.
The accelerating agent includes the raw material of following weight proportion：Candida fermented liquid：The lichen bacillus ferments liquid：
Cellumomonas flavigena zymotic fluid：Bacillus foecalis alkaligenes zymotic fluid：Gibberellin：Activated carbon：Dipotassium hydrogen phosphate=5：3：2：3：1：2：
1；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276；
The Cellumomonas flavigena is Cellumomonas flavigena（cellulomonas flavigena）ATCC
No.482；
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes（Alcaligenes faecalis）ATCC 31555；
The preparation method of the accelerating agent is：First by candidiasis, bacillus licheniformis, Cellumomonas flavigena,
Bacillus foecalis alkaligenes activates in a conventional manner respectively, then cultivates into bacterium solution viable count and reaches 108A/gram acquisition zymotic fluid,
By above-mentioned zymotic fluid according to mass ratio 5：3：2：3 mixing, according to weight proportion addition activated carbon, gibberellin and dipotassium hydrogen phosphate
To obtain the final product.
Survival rate is to 96.5% after transplanting 30 days, and all survive plant equal winter and resist cold；
The Chinese yew plant obtained through the method for the present invention, growing way is strong, disease-resistant, can pass the winter safely, efficiently solves red
The vacancy of beans China fir market resource, has broad application prospects.
The Chinese yew cultivated according to the method for the present invention, growth cycle is fast, and branches and leaves can be harvested at 2 years or so and are used to carry
Take taxol.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, or the seeds outside case study on implementation implement this method, this is to this
It is obvious for field technology personnel.Therefore, these modifications made without departing from theon the basis of the spirit of the present invention, change
Into or scope expansion, belong to the scope of protection of present invention.
Claims (2)
1. a kind of accelerating agent for Chinese yew root growth, it is characterised in that the accelerating agent includes following weight proportion
Raw material：Candida fermented liquid：The lichen bacillus ferments liquid：Cellumomonas flavigena zymotic fluid：Bacillus foecalis alkaligenes ferments
Liquid：Gibberellin：Activated carbon：Dipotassium hydrogen phosphate=5：3：2：3：1：2：1；
The candidiasis are candidiasis（Candida utilis）ATCC No.22023；
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276；
The Cellumomonas flavigena is Cellumomonas flavigena（Cellulomonas flavigena）ATCC No.482；
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes（Alcaligenes faecalis）ATCC No. 31555.
2. accelerating agent according to claim 1, it is characterised in that the preparation method of the accelerating agent is：First by false silk
Saccharomycete, bacillus licheniformis, Cellumomonas flavigena, Bacillus foecalis alkaligenes activate in a conventional manner respectively, then cultivate to
Viable count reaches 10 in bacterium solution8A/gram, zymotic fluid is obtained, above-mentioned zymotic fluid and activated carbon, gibberellin and dipotassium hydrogen phosphate are mixed
Close to obtain the final product.
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| CN1449251A (en) * | 2000-02-23 | 2003-10-15 | 吴羽化学工业株式会社 | Plant growth promoters and method of promoting plant growth |
| CN102344814A (en) * | 2011-07-20 | 2012-02-08 | 日照益康有机农业科技发展有限公司 | Saline-alkali soil improving agent and its preparation method |
| CN105331567A (en) * | 2015-12-21 | 2016-02-17 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1449251A (en) * | 2000-02-23 | 2003-10-15 | 吴羽化学工业株式会社 | Plant growth promoters and method of promoting plant growth |
| CN102344814A (en) * | 2011-07-20 | 2012-02-08 | 日照益康有机农业科技发展有限公司 | Saline-alkali soil improving agent and its preparation method |
| CN105331567A (en) * | 2015-12-21 | 2016-02-17 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter |
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