CN105331567A - Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter - Google Patents

Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter Download PDF

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CN105331567A
CN105331567A CN201510958358.XA CN201510958358A CN105331567A CN 105331567 A CN105331567 A CN 105331567A CN 201510958358 A CN201510958358 A CN 201510958358A CN 105331567 A CN105331567 A CN 105331567A
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bacillus amyloliquefaciens
seed
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plant growth
medium
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CN105331567B (en
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何月秋
吴毅歆
何鹏飞
何鹏搏
杨发祥
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Fermentation Research Center Yunnan Co Ltd
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Fermentation Research Center Yunnan Co Ltd
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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Abstract

The invention discloses bacillus amyloliquefaciens, a plant growth promoter and application of the plant growth promoter. The preservation number of the Bacillus amyloliquefaciens YN2010-KC is CGMCC No.5722. The plant growth promoter is prepared through fermentation of the bacillus amyloliquefaciens. By means of the bacillus amyloliquefaciens and the promoter, plant growth can be remarkably promoted, and yield increasing of crops is promoted. Production cost is low, the preparation technology is simple, the application method is simple and easy to implement, and the plant growth promoter can be directly used for seed soaking, seed stirring, root irrigation or plant soaking. The plant growth promoter can also serve as microbial fertilizer, is an environment-friendly biological preparation, can reduce environment pollution and can repair soil.

Description

A kind of bacillus amyloliquefaciens and promoting growth of plants preparation and application
Technical field
The invention belongs to microbial fertilizer technical field, particularly a kind of bacillus amyloliquefaciens, containing the microbial inoculum and preparation method thereof of bacillus amyloliquefaciens and the microbial inoculum prepared by bacillus amyloliquefaciens Promoting plant growth and in taking root application.
Background technology
For a long time, chemical fertilizer is used in a large number in agriculture production, although chemical fertilizer fertilizer efficiency is comparatively obvious, due to inorganic nutrients contained inside chemical fertilizer easily fix by compositions such as lime contained in soil, cause soil compaction, microorganism disappears, soil fertility declines, the harmful chemicals residue making Soil accumulation numerous, and disease and pest is rampant, and a large amount of using of chemical fertilizer also can cause severe contamination to water source, causes agroecological environment and environment for human survival to be all subject to serious threat.Meanwhile, excessive chemical fertilizer also can directly affects quality of agricultural product and output, reduces the market competitiveness of agricultural-food.
While negative impact served by chemical fertilizer band, microbial fertilizer receives the concern of people day by day, occupies more and more important role and importance in the Sustainable development of modern agriculture.Microbial fertilizer is live body fertilizer, containing a large amount of beneficial microorganism, after being manured into soil, a large amount of microorganism lived under optimum conditions can be actively movable, can activate the soil compaction because life-time service chemical fertilizer causes and residual n-p-k element, increase soil organic matter content, secretion tethelin stimulates plant growth, promotes root system development, improves the quality of farm crop, improve crop drought resistance, resistance against diseases, lay a good foundation for crop yield increases income.
Bacillus amyloliquefaciens (Bacillusamyloliquefaciens) is a kind in bacillus, is the advantage group of soil and plant Tiny ecosystem, is also a kind of beneficial microorganism.There is the throughput of stronger secondary metabolite, can plant growth hormones be secreted, as indolylacetic acid, Promoting plant growth; Can secretion of phytase and organic acid, phytic acid and mineral phosphorus in degraded soil; Can produce multiple antibacterial relevant meta-bolites, as proteolytic enzyme, bacillin, bacillus alkene, wind are plain together, and macrolide etc. all have strong restraining effect to fungus and bacterium, have the potentiality of practical application.
Summary of the invention
The object of the invention be to provide a kind of nontoxic, pollution-free, noresidue and safety microbial preparation Promoting plant growth, take root, survive and improve crop yield.
For achieving the above object, the technical scheme taked of the present invention is as follows:
1. a bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KC, its preserving number is: CGMCCNo.5722.
2. a preparation method for promoting growth of plants preparation, is characterized in that comprising the following steps:
(1) after bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 described in technical scheme 1 being activated, by 5 ~ 10% inoculum sizes, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, under culture temperature 35 DEG C, rotating speed 120rpm/min condition, 36 ~ 48h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2;
(2) by 10 ~ 12% inoculum sizes, primary seed solution is transferred to the secondary seed medium in seeding tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, air flow is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth in massfraction: starch 1.5 ~ 3%, bran powder 1 ~ 2%, yeast extract paste 0.05 ~ 0.3%, peptone 0.5 ~ 1%, ground phosphate rock 1 ~ 2%, MgSO 47H 2o0.01 ~ 0.05%, CaCl 20.1 ~ 0.4%, (NH 4) 2sO 40.01 ~ 0.04%, surplus is water, pH7.0 ~ 7.2;
(3) by 10 ~ 12% inoculum sizes, secondary seed solution is inoculated into the fermention medium in fermentor tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, air flow is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, You effect Jun Shuo≤1 × 10 alive of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 in the fermented liquid after cultivation 8during cfu/ml, packed by fermented liquid medicinal plastic bottle, be described promoting growth of plants preparation, described fermention medium is identical with step (2) described secondary seed medium.
3. a promoting growth of plants preparation, described promoting growth of plants preparation contains bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 described in technical scheme 1.
4. a promoting growth of plants preparation, prepares by the preparation method described in technical scheme 2.
5. the application of promoting growth of plants preparation on Promoting plant growth described in technical scheme 3 or 4.
6. according to technical scheme 5, be applied as the application of transplanting seed soaking, seed dressing, nursery, cuttage or forest.
7. being applied as according to technical scheme 5 is improving the application on crop yield.
Compared with prior art, the present invention has following beneficial effect:
1, bacterial strain of the present invention and preparation thereof can Promoting plant growths and promote increasing crop yield significantly.
In plant rooting of cuttings, its surviving rate, more than 95%, improves 30 ~ 40% than NAA root-inducing powder, and the surviving rate of transplanting tree, more than 93%, improves 40 ~ 50% than NAA root-inducing powder; Food crop seed dressing, immersion treatment are significantly increased production and growth promotion, and volume increase 10% average than clear water contrast, on average increases production 6.69% than FZB42 suspension agent; The vegetables such as tomato, cucumber, garlic are applied, on tomato, than clear water contrast volume increase 17.34%, than FZB42 suspension agent volume increase 5.48%; On cucumber, than clear water contrast volume increase 12.53%, than FZB42 suspension agent volume increase 5.24%; On garlic, its radical, plant fresh weight, plant height, than FZB42 suspension agent, increase by 6.57%, 6.07%, 4.92% respectively.Invention formulation is a kind of microbial fertilizer, is again a plant growth regulators.
2, bacterial strain of the present invention and preparation thereof are as plant-growth regulator, microbial fertilizer, avoid " three wastes " aborning and pollute, belong to environmentally friendly biotechnological formulation, and can reduce environmental pollution and energy rehabilitating soil, its preparation technology is simple, and cost is low.
Bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC of the present invention is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
Embodiment
Further illustrate below in conjunction with embodiment, each embodiment is ordinary method without specified otherwise.FZB42 suspension agent described in following examples is commercially available, is a kind of microbial fertilizer product containing bacillus amyloliquefaciens..
The acquisition of embodiment 1 bacillus amyloliquefaciens of the present invention (Bacillusamyloliquefaciens) YN2010-KC, preservation
1, the acquisition of bacillus amyloliquefaciens YN2010-KC of the present invention
1.1 material
Pick up from the soil around the Rhizosphere of Crops of Yunnan Prov Agriculture University Hou Shan farm.
1.2 medium preparing
LB solid medium and PSA substratum (potato sucrose nutrient agar) are melted, pours sterilizing culture dish respectively into, cooling, make LB and PSA dull and stereotyped.
1.3 separation and Culture
Adopting dilution plate partition method to be separated with plate streaking, is YN2010-KC by a Strain Designation of separation and purification gained.
2, the qualification of bacillus amyloliquefaciens YN2010-KC
2.1, Morphological Identification
Get bacterial strain YN2010-KC to cultivate on LB substratum, cultivate 36 hours, carry out morphologic observation qualification for 37 DEG C, thalline is shaft-like, end circle, and peritrichous, tool mobility, produce oval gemma; On LB and PSA flat board, bacterium colony circle, weak tea brown, bacterium colony central color slightly dark, protuberance, surface drying has fold.(eastern elegant pearl etc. are write with " common bacteria system identification handbook ", Science Press, calendar year 2001) in describe bacillus morphological specificity basically identical, tentatively judge that bacterial strain YN2010-KC belongs to genus bacillus (Bacillusspp.).
2.2, Physiology and biochemistry qualification
Bacterial strain gramstaining, spore staining, Starch Hydrolysis, glucose utilization, Citrate trianion utilization, V-P reaction, catalase reaction, nitrate reduction, gelatine liquefication etc. are all positive.
3, the preservation of bacillus amyloliquefaciens YN2010-KC
Through above-mentioned identify show be separated obtain bacterial strain be bacillus amyloliquefaciens (Bacillusamyloliquefaciens), its code name is YN2010-KC, described bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
The preparation of embodiment 2 promoting growth of plants preparation of the present invention, comprises the following steps:
(1) primary seed solution preparation
Bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 that-80 DEG C are preserved is seeded on LB liquid medium, cultivate 24h for 35 DEG C, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 that must activate, by 10% inoculum size, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, culture temperature 35 DEG C, under rotating speed 120rpm/min condition, 36h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2,
(2) by 10% inoculum size, primary seed solution is transferred to the secondary seed medium in seeding tank, at 35 DEG C, rotating speed 100rpm/min, air flow is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth in massfraction: starch 2%, bran powder 1%, yeast extract paste 0.3%, peptone 0.5%, ground phosphate rock 1%, MgSO 47H 2o0.02%, CaCl 20.25%, (NH 4) 2sO 40.03%, surplus is water, pH7.0 ~ 7.2, and described secondary seed medium, in 121 DEG C of sterilizing 30min, is waited to be cooled to 30 DEG C, access primary seed solution.
(3) by 10% inoculum size, secondary seed solution is inoculated into the fermention medium in fermentor tank, at 35 DEG C, rotating speed 100rpm/min, air flow is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, and in the fermented liquid after cultivation, the living bacteria count of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 1 × 10 8during cfu/ml, packed by fermented liquid medicinal plastic bottle, be described promoting growth of plants preparation, described fermention medium is identical with step (2) described secondary seed medium.
Promoting growth of plants preparation of the present invention prepares by the preparation method of the promoting growth of plants preparation described in embodiment 2.
The application of embodiment 3 promoting growth of plants preparation of the present invention on Promoting plant growth
Promoting growth of plants preparation of the present invention is hereinafter referred to as this product.
One, overall operation
1, soak seed
1. soak: soak crop seed 10 ~ 40 minutes with this product 500 ~ 1000 times of diluents, pull seed out, use after drying in the shade.
2, dress seed
Before sowing, with 500 ~ 1000 times of diluent seed dressings of this product, dry subsequently, this product diluent consumption is 1/50 of grain weight.
3, nursery
1. after planting: use 500 ~ 1000 times of diluent pourings of this product at once, later every 7-10 days, use 1 time, share 3-4 time;
2., after plantation: the seedling after transplanting, use 500 ~ 1000 times of diluent pourings of this product immediately, later every 7-10 days, use 1 time, share 3-4 time.
4, cuttage
1. nursery stock cuttage: the branch of Woody flower (as camellia, rhododendron, rose, crape myrtle, Lindley Butterflybush Herb etc.) and commodity trees (as Ramulus et folium taxi cuspidatae, Podocarpus macrophyllus, China fir, Dendrocalamus brandisii etc.) is cut into the segment of band 1-2 leaf bud, after soaking in this product 200 times of diluents 5-10 minute or soaking 20-30 minute in 500 times of diluents, cuttage is on seedbed, water this product 500 times of diluents subsequently, later every 7-10 days, use 1 time, share 3 times.
2. forest is transplanted: during large-scale commodity trees (as China fir, Taiwania flousiana, alder etc.) balled transplanting, doubly liquid irrigating root is diluted with this product 500-1000, before earthing, the soil of tree base portion is watered a little with this bacterium liquid, after earthing, irrigate with the bacterium liquid of same concentration again, within 5-7 days, water again once, share 3 times.
3. beat mud: break into mud with this product 100-500 times diluent, can be used for plant rooting of cuttings, also can transplant in naked sapling and use, before transplanting, sapling root be put into described mud, after being stained with described mud, directly transplant.
Two, specific examples
(1) as plant-growth regulator
In following product 200 times of diluents, the living bacteria count of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 5.0 × 10 5cfu/ml.
In this product 500 times of diluents, the living bacteria count of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 2.0 × 10 5cfu/ml.
In this product 100 diluent, the living bacteria count of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 1.0 × 10 6cfu/ml.
In this product 1000 times of liquid, the living bacteria count of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 1.0 × 10 5cfu/mL;
1. during plant rooting of cuttings, plant cutting is following commodity trees, flowers branch: taxusyunnanensis (Taxusyunnanensis), Taxus x media (Taxusmedia), southerm yew (Taxuschinensis), crape myrtle (Lagerstroemiaindica), camellia (Camelliajapomica), Podocarpus macrophyllus (Podocarpusmacrophyllus), China fir (Cunninghamialanceolata), Dendrocalamus brandisii (Dendrocalamusbrandisii), rhododendron (Rhododendronspp.), rose (Rosarugose), Lindley Butterflybush Herb (Buddlejalindleyana), branch is cut into the segment of band 1 ~ 2 leaf bud, soak 5-10 minute in this product 200 times of diluents after, cuttage is on seedbed, water this product 500 times of diluents subsequently, later every 7 days, water 1 time, water 3 times altogether, cuttage survival rate, all more than 95%, improves 30 ~ 40% than NAA root-inducing powder, the naphthalene acid solution of described NAA root-inducing powder to be concentration be 500 mg/litre.
2. forest is transplanted: during with following large-scale commodity trees balled transplanting, with this product 500 times dilution liquid irrigating root, before earthing, waters the soil of tree base portion a little, after earthing, then irrigate with this product of same concentration, within 6 days, water once again with this product, share 3 times.
3. mud is beaten: with this product 100 diluent (1.0 × 10 6cfu/ml) break into mud, for plant rooting of cuttings, also can transplant in naked sapling and use, before transplanting, sapling root be put into described mud, after being stained with described mud, directly transplant.
When playing mud transplanting, the surviving rate of China fir (Cunninghamialanceolata), Taiwania flousiana (Floustaiwania), alder (Longpedunclealder), orchid (CymbidiumPumilum), all more than 93%, improves 40 ~ 50% than above-mentioned NAA root-inducing powder.
When filling with root transplanting, the survival rate 85% of south China pine (Pinuskwangtungensis), improves more than 40% than blank.
4. corn (kind is single No. 4 of meeting) seed dressing:, test site is in sicheng village of The Big Dragon Pool township of Yuxi E Shan county of Yunnan Province.Experimental field soil is red soil, middle fertility.
3 process are established in test altogether:
Process 1: (concentration is 1.0 × 10 to the 1000 times of liquid seed dressings of this product 5cfu/mL).
(concentration is 1.0 × 10 to process 2:FZB42 suspension agent 1000 times of liquid 5cfu/mL).
Process 3: clear water seed dressing (CK).
Dress seed before sowing, dry, the bacterium liquid diluent consumption of clear water or above-mentioned process is 1/50 of grain weight.Level land, play pool fertilising and sowing.Each plot area 66.7m 2, repeat for 4 times, completely random district group arranges.Employing wide-and narrow-row is sowed, wide row space 80cm, little line-spacing 40cm, spacing in the rows 25cm.
Corn trials result (see table 1):
This product of table 1 is on the impact of corn growth proterties and output
Corn yield: this product (process 1) on average increases production 10.01% than contrast (process 3), on average increases production 6.69% than FZB42 suspension agent (process 2).
Growth traits: this product (process 1) on average improves 3.38% than the seedling rate of contrast (process 3), and plant height on average increases by 10.27%, and Ear height on average improves 28.07%, and fringe slightly on average increases by 7.59%, and spike length on average increases by 7.08%.This product (process 1) on average improves 1.03% than the seedling rate of FZB42 suspension agent (process 2), and plant height on average increases by 1.45%, and Ear height on average improves 3.67%, and fringe slightly on average increases by 4.61%, and spike length on average increases by 0.28%.
Show: this product is significantly higher than the process of FZB42 suspension agent and contrast on promotion corn growth and corn yield.
(2) as fertilizer
1) apply on vegetables
A, this product fill with root application on tomato, cucumber
The field growth-promoting effect of this product on tomato, cucumber
Contrast is FZB42 suspension agent, and tomato (kind is green prosperous 108 gold medal cups), cucumber (kind is winter hat) carry out growth promotion simultaneous test.
Tomato and cucumber test carry out in the heliogreenhouse of Chenggong, Kunming, Yunnan Province Vegetable Base.Greenhouse be steel-framed plastic greenhouse (long X is wide=63mx9m, 567m 2), experimental field soil is brown earth, and fertility is first-class.Establish 3 process altogether:
Process 1: (concentration is 2.0 × 10 to this product 500 times of liquid 5cfu/mL).
(concentration is 2.0 × 10 to process 2:FZB42 suspension agent 500 times of liquid 5cfu/mL).
Process 3: equivalent clear water contrast (CK).
Each process repeats for 3 times, random alignment.Plot area is 8.4m (1.2mx7m).Ridging plastic-film-covered cultivation, spacing in the rows 33cm, little line-spacing 40cm, wide row space 80cm.
This product of table 2 is on the impact of tomato, cucumber growth proterties and output
Tomato test-results shows: the tomato plant height of this product and FZB42 process, stem are thick, fruit mass and output all significantly or pole be significantly higher than contrast, this product 500 times of liquid cell productions reach 17.34% than contrast stimulation ratio, the 500 times of liquid volume increase 5.48% of this product 500 times of liquor ratio FZB42 suspension agents.
Cucumber test result shows: the cucumber plant height of this product and FZB42 process, stem are thick, fruit mass and output all significantly or pole be significantly higher than contrast.Compared with the control, this product 500 times of liquid cell production stimulation ratio are 12.53%, than the 500 times of liquid volume increase 5.24% of FZB42 suspension agent.
The growth-promoting effect of B, this product seed soaking garlic
Test is carried out in greenhouse, Yunnan Prov Agriculture University's teaching test farm.If 3 process, process 1: this product 1000 times of liquid (1.0 × 10 5cfu/mL), 2:FZB421000 times of liquid (1.0 × 10 is processed 5cfu/mL), 3 are processed: clear water blank (CK).Each process repeats for 3 times, and random alignment often repeats 50.After seed soaking 8h, be seeded in community planned in advance immediately, after planting each community is watered once by the equal water yield, is as the criterion to irrigate.Other management are carried out according to a conventional method.After 27d, measure its root length, plant fresh weight, plant height, radical
In greenhouse test, after 3 process seed soaking garlics, 3 be the results are shown in Table on the impact of its each biological indicator.
This product of table 3 is on the impact of seed soaking Garlic Growth
Note: different lowercase alphabets is shown in significant difference in the level of α=0.05, different capitalizations represents that difference is extremely remarkable in the level of α=0.01.
As can be known from Table 1:
Compared with the control, different treatment has impact in various degree to garlic the previous growth, and what effect was best is this product 1000 times of liquid.Compared with the control, with regard to plant height, this product 1000 times of liquid, a FZB421000 times liquid process increase by 27.26%, 21.30% respectively, all reach difference pole conspicuous level; Different treatment has impact in various degree to the radical of garlic, fresh weight, and aggregate performance is, radical is more, corresponding plant fresh weight higher (table 1).Reason is, fibrous root number increases, and is conducive to the absorption of nutritive substance, for growing of plant provides favourable condition.With regard to radical, this product 1000 times of liquid, a FZB421000 times liquid process increase by 11.94% (significant difference), 5.03% respectively; With regard to plant fresh weight, product 1000 times of liquid, a FZB421000 times liquid process increase by 60.0%, 50.85% respectively, all reach difference pole conspicuous level.
Compared with FZB421000 times of liquid (processing 2), with regard to radical, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 6.57%; With regard to plant fresh weight, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 6.07%; With regard to plant height, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 4.92%.
(3) growth-promoting effect of product of the present invention on wheat
Test is located at Yunnan Prov Agriculture University in the school, and 3 process are established in test altogether, process 1: (concentration is 1.0 × 10 to this product 1000 times of liquid 5cfu/mL), (concentration is 1.0 × 10 to process 2:FZB42 suspension agent 1000 times of liquid 5cfu/mL), 3 are processed: blank (CK) is clear water process.Get each 10mL of above-mentioned different treatment and put into the culture dish marked respectively, again sterile for surface wheat breed Chuanmai 107 is put into respectively after culture dish carries out seed dressing 10min, dry, seed is put into the culture dish that soil is housed, each culture dish 20 seeds, each process repeats for 3 times, totally 3 process.Water clear water according to soil dry-wet situation later, to keep ground moistening, indoor cultivation, 5d " Invest, Then Investigate " wheat plant upgrowth situation.Measure plant fresh weight respectively, plant height, root are long, record experimental result.
The growing state of this product of table 4 seed dressing wheat
Note: different lowercase alphabets is shown in significant difference in the level of α=0.05, different capitalizations represents that difference is extremely remarkable in the level of α=0.01.
With regard to seed dressing, different treatment has impact (table 4) in various degree to Wheat Development each biological indicator in early stage, compared with contrasting with clear water, with regard to plant fresh weight, this product 1000 times of liquid, a FZB421000 times liquid process increase by 37.5% respectively, 12.5%, all reach the significance level of difference.With regard to plant height, this product 1000 times of liquid, a FZB421000 times liquid process increase by 34.14%, 13.69% respectively, all reach the significance level of difference.With regard to root is long, this product 1000 times of liquid, a FZB421000 times liquid process increase by 20.71% (significant difference), 2.24% respectively.
Compared with FZB421000 times of liquid (processing 2), with regard to plant fresh weight, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 22.22%; With regard to plant height, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 17.99%; With regard to root is long, this product 1000 times of liquor ratio FZB421000 times of liquid increase by 18.06%.

Claims (7)

1. a bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KC, its preserving number is: CGMCCNo.5722.
2. a preparation method for promoting growth of plants preparation, is characterized in that comprising the following steps:
(1) after bacillus amyloliquefaciens according to claim 1 (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 being activated, by 5 ~ 10% inoculum sizes, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, under culture temperature 35 DEG C, rotating speed 120rpm/min condition, 36 ~ 48h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2;
(2) by 10 ~ 12% inoculum sizes, primary seed solution is transferred to the secondary seed medium in seeding tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, air flow is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth in massfraction: starch 1.5 ~ 3%, bran powder 1 ~ 2%, yeast extract paste 0.05 ~ 0.3%, peptone 0.5 ~ 1%, ground phosphate rock 1 ~ 2%, MgSO 47H 2o0.01 ~ 0.05%, CaCl 20.1 ~ 0.4%, (NH 4) 2sO 40.01 ~ 0.04%, surplus is water, pH7.0 ~ 7.2;
(3) by 10 ~ 12% inoculum sizes, secondary seed solution is inoculated into the fermention medium in fermentor tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, air flow is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, You effect Jun Shuo≤1 × 10 alive of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 in the fermented liquid after cultivation 8during cfu/ml, fermented liquid medicinal plastic bottle is packed, be described promoting growth of plants preparation; Described fermention medium is identical with step (2) described secondary seed medium.
3. a promoting growth of plants preparation, is characterized in that: described promoting growth of plants preparation contains bacillus amyloliquefaciens according to claim 1 (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722.
4. a promoting growth of plants preparation, is characterized in that: described promoting growth of plants preparation prepares by preparation method according to claim 2.
5. the application of promoting growth of plants preparation on Promoting plant growth described in claim 3 or 4.
6. be according to claim 5ly applied as the application of transplanting seed soaking, seed dressing, nursery, cuttage or forest.
7. according to claim 5 being applied as is improving the application on crop yield.
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CN110982730A (en) * 2019-10-23 2020-04-10 海南大学 Microbial fertilizer, preparation method and application
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CN105660709A (en) * 2016-02-22 2016-06-15 杭州富阳飞博科技有限公司 Accelerant for root growth of taxus chinensis and method for applying accelerant to crossbreeding
CN105660709B (en) * 2016-02-22 2018-04-27 杭州富阳飞博科技有限公司 A kind of method for the accelerating agent of Chinese yew root growth and its for crossbreeding
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CN105746033B (en) * 2016-03-02 2018-09-25 江苏省农业科学院 Applications of the bacillus amyloliquefaciens B-1619 in promoting facilities vegetable growth
CN106922738A (en) * 2017-04-27 2017-07-07 四川省农业科学院植物保护研究所 The method that biocontrol bacterial strain bacillus amyloliquefaciens Bam22 is used for increasing yield of water melons
CN106922738B (en) * 2017-04-27 2019-09-13 四川省农业科学院植物保护研究所 The method that biocontrol bacterial strain bacillus amyloliquefaciens Bam22 is used for increasing yield of water melons
CN108377819A (en) * 2018-02-05 2018-08-10 云南省烟草公司普洱市公司 A method of prevention tobacco black shank
CN108377774A (en) * 2018-04-20 2018-08-10 金春兰 A kind of cuttage breeding method of Impatiens Hybriden
CN109628342A (en) * 2018-12-26 2019-04-16 大连雪龙都市农业有限公司 A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application
CN110982730A (en) * 2019-10-23 2020-04-10 海南大学 Microbial fertilizer, preparation method and application
CN110982730B (en) * 2019-10-23 2023-04-18 海南大学 Microbial fertilizer, preparation method and application
CN113142018A (en) * 2021-04-30 2021-07-23 上海欧思景观设计工程有限公司 Podocarpus macrophyllus planting soil

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